WO2000047760A3 - Atm kinase modulation for screening and therapies - Google Patents

Atm kinase modulation for screening and therapies Download PDF

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Publication number
WO2000047760A3
WO2000047760A3 PCT/US2000/003386 US0003386W WO0047760A3 WO 2000047760 A3 WO2000047760 A3 WO 2000047760A3 US 0003386 W US0003386 W US 0003386W WO 0047760 A3 WO0047760 A3 WO 0047760A3
Authority
WO
WIPO (PCT)
Prior art keywords
atm
screening
kinase
assay system
atm kinase
Prior art date
Application number
PCT/US2000/003386
Other languages
French (fr)
Other versions
WO2000047760A9 (en
WO2000047760A2 (en
Inventor
Michael Kastan
Chirstine Canman
Seong-Tae Kim
Dae-Sik Lim
Original Assignee
St Jude Childrens Res Hospital
Univ Johns Hopkins
Michael Kastan
Chirstine Canman
Kim Seong Tae
Lim Dae Sik
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/248,061 external-priority patent/US6387640B1/en
Application filed by St Jude Childrens Res Hospital, Univ Johns Hopkins, Michael Kastan, Chirstine Canman, Kim Seong Tae, Lim Dae Sik filed Critical St Jude Childrens Res Hospital
Priority to AU38570/00A priority Critical patent/AU3857000A/en
Publication of WO2000047760A2 publication Critical patent/WO2000047760A2/en
Publication of WO2000047760A3 publication Critical patent/WO2000047760A3/en
Publication of WO2000047760A9 publication Critical patent/WO2000047760A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases

Abstract

The present invention relates to identification of the consensus sequence phosphorylated by ATM kinase. This, in turn, permitted identification of ATM kinase target proteins, and development of a convenient assay system for ATM kinase phosphorylation using fusion polypeptides as substrates. The assay system is adaptable to screening for ATM modulators, particularly inhibitors. In a specific embodiment, the substrate recognition sequence and mutagenized variants of this sequence were incorporated in a GST fusion protein and assayed for phosphorylation by ATM kinase. This assay system is useful in screening for ATM inhibitors. ATM function assays were validated using an ATM-kinase dead dominant-negative mutant.
PCT/US2000/003386 1999-02-10 2000-02-09 Atm kinase modulation for screening and therapies WO2000047760A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU38570/00A AU3857000A (en) 1999-02-10 2000-02-09 Atm kinase modulation for screening and therapies

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/248,061 US6387640B1 (en) 1999-02-10 1999-02-10 ATM kinase modulation for screening and therapies
US09/248,061 1999-02-10
US09/400,653 1999-09-21
US09/400,653 US6348311B1 (en) 1999-02-10 1999-09-21 ATM kinase modulation for screening and therapies

Publications (3)

Publication Number Publication Date
WO2000047760A2 WO2000047760A2 (en) 2000-08-17
WO2000047760A3 true WO2000047760A3 (en) 2000-11-30
WO2000047760A9 WO2000047760A9 (en) 2001-03-01

Family

ID=26939088

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/003386 WO2000047760A2 (en) 1999-02-10 2000-02-09 Atm kinase modulation for screening and therapies

Country Status (3)

Country Link
US (2) US6348311B1 (en)
AU (1) AU3857000A (en)
WO (1) WO2000047760A2 (en)

Families Citing this family (25)

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GB9714971D0 (en) 1997-07-16 1997-09-24 Cancer Res Campaign Tech Assays,therapeutic methods and means
JP2000325086A (en) * 1999-05-21 2000-11-28 Igaku Seibutsugaku Kenkyusho:Kk Assay of activity of phosphorylase
EP1290214A2 (en) * 2000-05-30 2003-03-12 Kudos Pharmaceuticals Limited Inhibiting retroviral integration by targeting the atm pathway
GB0119863D0 (en) * 2001-08-14 2001-10-10 Cancer Res Campaign Tech DNA-PK inhibitors
GB0119865D0 (en) * 2001-08-14 2001-10-10 Cancer Res Campaign Tech DNA-PK inhibitors
WO2003028640A2 (en) * 2001-10-03 2003-04-10 Vanderbilt University In vivo panning for ligands to radiation-induced molecules
US7049313B2 (en) * 2002-02-25 2006-05-23 Kudos Pharmaceuticals Ltd. ATM inhibitors
AU2003229689A1 (en) * 2002-04-15 2003-10-27 Glaxo Group Limited A method of identifying a modudator for a serine/theronine kinase
US7166716B2 (en) 2002-06-06 2007-01-23 The Burnham Institute ATM related kinase ATX, nucleic acids encoding same and methods of use
US6916627B2 (en) * 2002-11-27 2005-07-12 St. Jude Children's Research Hospital ATM kinase compositions and methods
US7108992B2 (en) * 2002-11-27 2006-09-19 St. Jude Children's Research Hospital ATM kinase compositions and methods
US20070099186A1 (en) * 2003-05-01 2007-05-03 D Adda Di Fagagna Fabrizio Methods and means for the treatment of disorders associated with cellular senescence
MXPA06001599A (en) * 2003-08-13 2006-05-19 Kudos Pharm Ltd Aminopyrones and their use as atm inhibitors.
CA2580229A1 (en) 2004-09-20 2006-03-30 Kudos Pharmaceuticals Limited Dna-pk inhibitors
TW200638938A (en) * 2005-02-09 2006-11-16 Kudos Pharm Ltd ATM inhibitors
AR053358A1 (en) * 2005-04-15 2007-05-02 Cancer Rec Tech Ltd DNA INHIBITORS - PK
UY29774A1 (en) * 2005-08-31 2007-03-30 Kudos Pharm Ltd ATM INHIBITOR
WO2007075655A2 (en) 2005-12-19 2007-07-05 Northwestern University Compositions and methods for altering rnai
US20080279866A1 (en) * 2006-08-25 2008-11-13 The Brigham And Women's Hospital, Inc. Induction of immunosuppression by inhibition of ATM
WO2008100997A2 (en) * 2007-02-14 2008-08-21 Prelief Inc. Methods for treating or preventing hemorrhagic cystitis using a glycerophosphate salt
JP2010527982A (en) * 2007-05-25 2010-08-19 アストラゼネカ・アクチエボラーグ Combination of checkpoint kinase (CHK) and telangiectasia mutation (ATM) inhibitor for the treatment of cancer
UY31232A1 (en) * 2007-07-19 2009-03-02 COMPOUNDS DERIVED FROM DIBENZOTIFENILAMINO-CROMEN-4-ACTIVE WAVES REPLACED AND ITS ISOMERS AND APPLICATIONS
US20100210600A1 (en) * 2009-02-19 2010-08-19 Prelief Inc. Methods and Compositions for Treating Urogenital Disorders
US9644037B2 (en) 2013-10-18 2017-05-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Antibodies that specifically bind ataxia telangiectasia-mutated and RAD3-related kinase phosphorylated at position 1989 and their use
JP6524108B2 (en) 2014-02-17 2019-06-05 ユニヴェルシテ クロード ベルナール リヨン １ Predictive methods for characterizing patient radiosensitivity and tissue response to therapeutic ionizing radiation

Citations (2)

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WO1997018323A2 (en) * 1995-11-16 1997-05-22 Icos Corporation Cell-cycle checkpoint phosphatidylinositol- (pik-) related kinases, genes coding therefor and methods for assaying and modulating enzymatic activity
GB2327498A (en) * 1997-07-16 1999-01-27 Cancer Res Campaign Tech Purification of ATM, or homologues thereof, and assays involving the same

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US5756288A (en) 1995-05-16 1998-05-26 Ramot-University Of Authority For Applied Research And Industrial Dev. Ltd. Ataxia-telangiectasai gene
US5728807A (en) 1995-05-16 1998-03-17 Ramot-University Authority For Applied Research And Industrial Development, Ltd. Mutated proteins associated with ataxia-telangiectasia
US5777093A (en) 1995-05-16 1998-07-07 Ramot-University Authority For Applied Research & Industrial Development Ltd. cDNAs associated with ataxia-telangiectasia
CA2292540A1 (en) 1997-06-06 1998-12-10 Jann N. Sarkaria Screening for phosphatidylinositol related-kinase inhibitors
AU8068198A (en) 1997-06-13 1998-12-30 President And Fellows Of Harvard College Methods for treating human cancers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997018323A2 (en) * 1995-11-16 1997-05-22 Icos Corporation Cell-cycle checkpoint phosphatidylinositol- (pik-) related kinases, genes coding therefor and methods for assaying and modulating enzymatic activity
GB2327498A (en) * 1997-07-16 1999-01-27 Cancer Res Campaign Tech Purification of ATM, or homologues thereof, and assays involving the same

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
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BASKARAN, R. ET AL.: "Ataxiateangiectasia mutant protein activates c-Abl tyrosine kinase in response to ionizing radiation", NATURE, vol. 387, 29 May 1997 (1997-05-29), pages 516 - 519, XP000885379 *
FLAGGS, G. ET AL: "Atm-dependent interactions of a mammalian chk1 homolog with meiotic chromosomes", CURRENT BIOLOGY,GB,CURRENT SCIENCE,, vol. 7, no. 12, 1 December 1997 (1997-12-01), pages 977 - 986, XP002087728, ISSN: 0960-9822 *
KIM, S.T. ET AL.: "Substrate Specificities and Identification of Putative Substrates of ATM Kinase Family Members", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 53, 31 December 1999 (1999-12-31), pages 37538 - 37543, XP000934014 *
SANCHEZ Y ET AL: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25 [see comments]", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 277, no. 5331, 5 September 1997 (1997-09-05), pages 1497 - 1501, XP002087723, ISSN: 0036-8075 *
SCOTT, S.P. ET AL.: "Cloning and Expression of the Ataxia-Telangiectasia Gene in Baculovirus", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 245, no. 1, 1998, pages 144 - 148, XP000929922 *

Also Published As

Publication number Publication date
US20030022263A1 (en) 2003-01-30
WO2000047760A9 (en) 2001-03-01
AU3857000A (en) 2000-08-29
US6348311B1 (en) 2002-02-19
WO2000047760A2 (en) 2000-08-17

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