WO1999064559A3 - Dispositif de culture de cellules organiques et d'etude de leur activite electrophysiologique et membrane utilisee dans un tel dispositif - Google Patents

Dispositif de culture de cellules organiques et d'etude de leur activite electrophysiologique et membrane utilisee dans un tel dispositif Download PDF

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Publication number
WO1999064559A3
WO1999064559A3 PCT/CH1999/000243 CH9900243W WO9964559A3 WO 1999064559 A3 WO1999064559 A3 WO 1999064559A3 CH 9900243 W CH9900243 W CH 9900243W WO 9964559 A3 WO9964559 A3 WO 9964559A3
Authority
WO
WIPO (PCT)
Prior art keywords
support
cells
electrophysiological activity
studying
cell culture
Prior art date
Application number
PCT/CH1999/000243
Other languages
English (en)
Other versions
WO1999064559A2 (fr
Inventor
Claude Haenni
Luc Stoppini
Original Assignee
Claude Haenni
Luc Stoppini
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Claude Haenni, Luc Stoppini filed Critical Claude Haenni
Priority to AU40279/99A priority Critical patent/AU4027999A/en
Priority to JP2000553549A priority patent/JP2002517225A/ja
Priority to AT99923354T priority patent/ATE236399T1/de
Priority to US09/701,956 priority patent/US6689594B1/en
Priority to DE69906574T priority patent/DE69906574T2/de
Priority to IL14017499A priority patent/IL140174A/xx
Priority to EP99923354A priority patent/EP1133691B1/fr
Publication of WO1999064559A2 publication Critical patent/WO1999064559A2/fr
Publication of WO1999064559A3 publication Critical patent/WO1999064559A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • G01N33/4836—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures using multielectrode arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Optics & Photonics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Le dispositif comporte un support (11) dans l'épaisseur duquel est réalisée une chambre d'alimentation (12) ayant un conduit d'entrée (13) de liquide nutritif et un conduit d'évacuation (14) de ce liquide. Une capsule (22) pouvant recevoir des cellules organiques est disposée sur le support (11). La chambre d'alimentation (12) et le support (11) sont séparés par une membrane poreuse (16) comportant un réseau d'électrodes (17) disposées de façon à pouvoir être en contact avec différentes zones de l'amas de cellules et permettre ainsi d'analyser leur activité électrophysiologique. Ce dispositif permet d'augmenter la durée de vie des cellules et d'effectuer des analyses de façon simple, efficace et sans nuire à l'organisation des cellules.
PCT/CH1999/000243 1998-06-08 1999-06-04 Dispositif de culture de cellules organiques et d'etude de leur activite electrophysiologique et membrane utilisee dans un tel dispositif WO1999064559A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU40279/99A AU4027999A (en) 1998-06-08 1999-06-04 Device for organic cell culture and for studying their electrophysiological activity and membrane used in said device
JP2000553549A JP2002517225A (ja) 1998-06-08 1999-06-04 有機細胞を培養し、かつこの細胞の電気生理学的活性度を研究するための装置、およびこの装置で使用する膜
AT99923354T ATE236399T1 (de) 1998-06-08 1999-06-04 Vorrichtung zum züchten von organischen zellen und erforschung ihrer elektrophysiologischen aktivität und dabei verwendete membran
US09/701,956 US6689594B1 (en) 1998-06-08 1999-06-04 Device for organic cell culture and for studying their electrophysiological activity and membrane used in said device
DE69906574T DE69906574T2 (de) 1998-06-08 1999-06-04 Vorrichtung zum züchten von organischen zellen und erforschung ihrer elektrophysiologischen aktivität und dabei verwendete membran
IL14017499A IL140174A (en) 1998-06-08 1999-06-04 Device for organic cell cultures and for studying their electrophysiological activity and membrane used in said device
EP99923354A EP1133691B1 (fr) 1998-06-08 1999-06-04 Dispositif de culture de cellules organiques et d'etude de leur activite electrophysiologique et membrane utilisee dans un tel dispositif

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR98/07596 1998-06-08
FR9807596A FR2779443B1 (fr) 1998-06-08 1998-06-08 Dispositif de culture de cellules organiques et d'etude de leur activite electrophysiologique et membrane utilisee dans un tel dispositif

Publications (2)

Publication Number Publication Date
WO1999064559A2 WO1999064559A2 (fr) 1999-12-16
WO1999064559A3 true WO1999064559A3 (fr) 2001-07-12

Family

ID=9527465

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CH1999/000243 WO1999064559A2 (fr) 1998-06-08 1999-06-04 Dispositif de culture de cellules organiques et d'etude de leur activite electrophysiologique et membrane utilisee dans un tel dispositif

Country Status (10)

Country Link
US (1) US6689594B1 (fr)
EP (1) EP1133691B1 (fr)
JP (1) JP2002517225A (fr)
AT (1) ATE236399T1 (fr)
AU (1) AU4027999A (fr)
DE (1) DE69906574T2 (fr)
ES (1) ES2195572T3 (fr)
FR (1) FR2779443B1 (fr)
IL (1) IL140174A (fr)
WO (1) WO1999064559A2 (fr)

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US7485454B1 (en) * 2000-03-10 2009-02-03 Bioprocessors Corp. Microreactor
IL136232A0 (en) * 2000-05-18 2001-05-20 Bar Ilan University Res Author Measurements of enzymatic activity in a single, individual cell in population
EP1406086A4 (fr) * 2001-06-05 2007-10-31 Matsushita Electric Ind Co Ltd Capteur de detection de signaux pourvu de multiples electrodes
US20050014201A1 (en) * 2001-10-25 2005-01-20 Mordechai Deuthsch Interactive transparent individual cells biochip processor
JP4370082B2 (ja) * 2002-08-26 2009-11-25 独立行政法人科学技術振興機構 神経細胞培養マイクロチャンバー
IL154677A0 (en) 2003-02-27 2003-09-17 Univ Bar Ilan A method and apparatus for manipulating an individual cell
WO2004113492A1 (fr) * 2003-06-26 2004-12-29 Molecular Cytomics Ltd. Materiaux ameliores pour la construction de puces cellulaires, couvertures de puces cellulaires, revetements de puces cellulaires, puces cellulaires traitees et leurs utilisations
US8597597B2 (en) * 2003-06-26 2013-12-03 Seng Enterprises Ltd. Picoliter well holding device and method of making the same
US7888110B2 (en) 2003-06-26 2011-02-15 Seng Enterprises Ltd. Pico liter well holding device and method of making the same
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate
WO2005008626A1 (fr) * 2003-07-11 2005-01-27 University Of North Carolina At Chapel Hill Procedes et systemes de commande d'un ordinateur au moyen d'une image video et de combinaison de l'image video a un ordinateur
US20050064524A1 (en) * 2003-08-11 2005-03-24 Mordechai Deutsch Population of cells utilizable for substance detection and methods and devices using same
DE602004021001D1 (de) 2003-08-21 2009-06-18 Monsanto Technology Llc Fettsäuredesaturasen aus primula
BRPI0506610A (pt) * 2004-02-03 2007-05-02 Chemagis Ltd montelucaste sódico amorfo, processo de preparação de montelucaste sódico amorfo, composição farmacêutica, método de tratamento utilizando a administração de montelucaste sódico amorfo, co-precipitado amorfo de montelucaste sódico e processo de preparação de co-precipitado amorfo de montelucaste sódico
US7403647B2 (en) * 2004-09-13 2008-07-22 Seng Enterprises Ltd. Method for identifying an image of a well in an image of a well-bearing component
EP1763665A1 (fr) * 2004-07-07 2007-03-21 Seng Enterprises Limited Procede et dispositif d'identification d'une image d'un puits dans une image de composant porteur de puits
EP1781404A2 (fr) * 2004-08-25 2007-05-09 Seng Enterprises Limited Procede et dispositif destines a isoler des cellules
US20080090739A1 (en) * 2004-09-30 2008-04-17 Van Beuningen Marinus G J Masked Solid Porous Supports Allowing Fast And Easy Reagent Exchange To Accelerate Electrode-Based Microarrays
ES2352344T3 (es) 2005-01-25 2011-02-17 Seng Enterprises Limited Dispositivo de microfluido para estudio de células.
GB0512214D0 (en) 2005-06-15 2005-07-27 Capsant Neurotechnologies Ltd Method
GB0512216D0 (en) * 2005-06-15 2005-07-27 Capsant Neurotechnologies Ltd Device
US20070141555A1 (en) * 2005-10-11 2007-06-21 Mordechai Deutsch Current damper for the study of cells
WO2007052245A1 (fr) * 2005-11-03 2007-05-10 Seng Enterprises Ltd. Procédé et dispositif d’étude de cellules vivantes flottantes
US20060223999A1 (en) * 2006-05-10 2006-10-05 Chemagis Ltd. Process for preparing montelukast and precursors thereof
JP2009544947A (ja) * 2006-07-24 2009-12-17 バイオツァー エントヴィックルングス−ゲーエムベーハー 細胞に関するオンライン測定のための装置
DE102007016629A1 (de) * 2007-04-05 2008-10-09 Micronas Gmbh Sensor zur Erfassung eines toxischen oder gefährlichen Gasgemisches und Betriebsverfahren
WO2009081409A2 (fr) 2007-12-26 2009-07-02 Seng Enterprises Ltd. Dispositif pour l'étude de cellules vivantes
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells
JP5200888B2 (ja) * 2008-11-21 2013-06-05 大日本印刷株式会社 パターン細胞培養用器具
US20140045252A1 (en) * 2011-04-28 2014-02-13 Hitachi, Ltd. Cell cultivation container and cell culturing apparatus
CN102680526B (zh) * 2012-05-16 2014-07-02 清华大学 单细胞阵列微芯片及其制造、电测量和电穿孔方法
CN103630579A (zh) * 2013-02-27 2014-03-12 中国科学院电子学研究所 细胞阻抗分析的芯片及仪器
EP4170014A1 (fr) 2021-10-21 2023-04-26 Alpvision SA Système microfluidique pour la mesure et/ou la stimulation électrique robuste à long terme de cultures cellulaires

Citations (3)

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SU1124022A1 (ru) * 1983-04-15 1984-11-15 Специальное конструкторское бюро биологического приборостроения АН СССР Камера дл регистрации электрофизиологических характеристик и фиксации потенциала мембраны биологического объекта
EP0689051A2 (fr) * 1994-06-13 1995-12-27 Matsushita Electric Industrial Co., Ltd. Appareil de mesure du potentiel cellulaire avec plusieurs microélectrodes
US5759846A (en) * 1995-04-12 1998-06-02 Chemodyne S.A. Device for the study of organotypic cultures and its uses in electrophysiology and biochemistry

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Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
SU1124022A1 (ru) * 1983-04-15 1984-11-15 Специальное конструкторское бюро биологического приборостроения АН СССР Камера дл регистрации электрофизиологических характеристик и фиксации потенциала мембраны биологического объекта
EP0689051A2 (fr) * 1994-06-13 1995-12-27 Matsushita Electric Industrial Co., Ltd. Appareil de mesure du potentiel cellulaire avec plusieurs microélectrodes
US5759846A (en) * 1995-04-12 1998-06-02 Chemodyne S.A. Device for the study of organotypic cultures and its uses in electrophysiology and biochemistry

Non-Patent Citations (1)

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Title
DATABASE WPI Section Ch Week 8523, Derwent World Patents Index; Class D16, AN 85-139721, XP002095669 *

Also Published As

Publication number Publication date
FR2779443B1 (fr) 2000-08-04
DE69906574T2 (de) 2004-01-29
EP1133691A2 (fr) 2001-09-19
WO1999064559A2 (fr) 1999-12-16
AU4027999A (en) 1999-12-30
ATE236399T1 (de) 2003-04-15
US6689594B1 (en) 2004-02-10
DE69906574D1 (de) 2003-05-08
EP1133691B1 (fr) 2003-04-02
IL140174A (en) 2003-12-10
ES2195572T3 (es) 2003-12-01
FR2779443A1 (fr) 1999-12-10
JP2002517225A (ja) 2002-06-18
IL140174A0 (en) 2002-02-10

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