WO1999037753A1 - Enzyme catalyzed therapeutic agents - Google Patents
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- WO1999037753A1 WO1999037753A1 PCT/US1999/001332 US9901332W WO9937753A1 WO 1999037753 A1 WO1999037753 A1 WO 1999037753A1 US 9901332 W US9901332 W US 9901332W WO 9937753 A1 WO9937753 A1 WO 9937753A1
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- 0 C*(C(*(C)(*)C=C1*)=O)C1=O Chemical compound C*(C(*(C)(*)C=C1*)=O)C1=O 0.000 description 3
- GOVXKUCVZUROAN-UHFFFAOYSA-N CCc1c[nH]c2ccccc12 Chemical compound CCc1c[nH]c2ccccc12 GOVXKUCVZUROAN-UHFFFAOYSA-N 0.000 description 1
- JGISQGJDPMKSPH-UVXRARDASA-N C[C@@H](C(OC)=O)NP(OCC([C@H](C1)O)OC1N(C=C(C(N1)=C)C#CCOP(N2CC2)(N2CC2)=O)C1=O)(Oc1ccccc1)=O Chemical compound C[C@@H](C(OC)=O)NP(OCC([C@H](C1)O)OC1N(C=C(C(N1)=C)C#CCOP(N2CC2)(N2CC2)=O)C1=O)(Oc1ccccc1)=O JGISQGJDPMKSPH-UVXRARDASA-N 0.000 description 1
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- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/555—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound pre-targeting systems involving an organic compound, other than a peptide, protein or antibody, for targeting specific cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/555—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound pre-targeting systems involving an organic compound, other than a peptide, protein or antibody, for targeting specific cells
- A61K47/556—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound pre-targeting systems involving an organic compound, other than a peptide, protein or antibody, for targeting specific cells enzyme catalyzed therapeutic agent [ECTA]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/66—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid the modifying agent being a pre-targeting system involving a peptide or protein for targeting specific cells
- A61K47/67—Enzyme prodrug therapy, e.g. gene directed enzyme drug therapy [GDEPT] or VDEPT
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
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- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/645—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having two nitrogen atoms as the only ring hetero atoms
- C07F9/6509—Six-membered rings
- C07F9/6512—Six-membered rings having the nitrogen atoms in positions 1 and 3
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- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
Definitions
- the present invention relates to the field of drug discovery and specifically, the design of prodrugs which are substrates for an intracellular enzyme critical to resistance to therapeutics in pathological cells and converted to a cell toxin by the intracellular enzyme.
- Cancer is one of the most commonly fatal human diseases worldwide. Treatment with anticancer drugs is an option of steadily increasing importance, especially for systemic malignancies or for metastatic cancers which have passed the state of surgical curability. Unfortunately, the subset of human cancer types that are amenable to curative treatment today is still rather small (Haskell, CM. eds. (1995), p. 32). Progress in the development of drugs that can cure human cancer is slow. The heterogeneity of malignant tumors with respect to their genetics, biology and biochemistry as well as primary or treatment-induced resistance to therapy mitigate against curative treatment.
- the major classes of agents include the alkylating agents, antitumor antibiotics, plant alkaloids, antimetabolites, hormonal agonists and antagonists, and a variety of miscellaneous agents. See Haskell, CM., ed., (1995) and Dorr, R.T. and Von Hoff, D.D., eds. (1994). 3
- the classic alkylating agents are highly reactive compounds that have the ability to substitute alkyl groups for the hydrogen atoms of certain organic compounds. Alkylation of nucleic acids, primarily DNA, is the critical cytotoxic action for most of these compounds. The damage they cause interferes with DNA replication and RNA transcription.
- the classic alkylating agents include mechlorethamine, chlorambucil, melphalan, cyclophosphamide, ifosfamide, thiotepa and busulfan.
- a number of nonclassic alkylating agents also damage DNA and proteins, but through diverse and complex mechanisms, such as methylation or chloroethylation, that differ from the classic alkylators.
- the nonclassic alkylating agents include dacarbazine, carmustine, lomustine, cisplatin, carboplatin, procarbazine and altretamine.
- antitumor drugs are natural products of various strains of the soil fungus Streptomyces. They produce their tumoricidal effects by one or more mechanisms. All of the antibiotics are capable of binding DNA, usually by intercalation, with subsequent unwinding of the helix. This distortion impairs the ability of the DNA to serve as a template for DNA synthesis, RNA synthesis, or both. These drugs may also damage DNA by the formation of free radicals and the chelation of important metal ions. They may also act as inhibitors of topoisomerase II, an enzyme critical to cell division.
- the antimetabolites are structural analogs of normal metabolites that are required for cell function and replication. They typically work by interacting with cellular enzymes.
- methotrexate 5-fluorouracil
- FUDR floxuridine
- cytarabine 6-mercaptopurine
- 6-thioguanine 6-thioguanine
- deoxycoformycin fludarabine
- 2-chlorodeoxyadenosine 2-chlorodeoxyadenosine
- Endocrine manipulation is an effective therapy for several forms of neoplastic disease.
- hormones and hormone antagonists have been developed for potential use in oncology. Examples of available hormonal agents are diethylstilbestrol, tamoxifen, megestrol acetate, dexamethasone, prednisone, aminoglutethimide, leuprolide, goserelin, flutamide, and octreotide acetate.
- Toxicity results because many normal cell types (e.g., colon epithelium, hematopoietic cells) have a high proliferative rate. Because of host toxicity, treatment has to be discontinued at dose levels that are well below the dose that would be required to kill all viable tumor cells.
- Another side effect associated with present day therapies is the toxic effect of the chemotherapeutic on the normal host tissues that are the most rapidly dividing, such as the bone marrow, gut mucosa and cells of the lymphoid system.
- the agents also exert a variety of other adverse effects, including neurotoxicity; negative effects on sexuality and gonadal function; and cardiac, pulmonary, pancreatic and hepatic toxicities; vascular and hypersensitivity reactions, and dermatological reactions.
- Hemato logic toxicity is the most dangerous form of toxicity for many of the antineoplastic drugs used in clinical practice. Its most common form is neutropenia, with an attendant high risk of infection, although thrombocytopenia and bleeding may also occur and be life threatening. Chemotherapy may also induce qualitative defects in the function of both polymorphonuclear leukocytes and platelets. The hematopoietic growth factors have been developed to address these important side effects. Wilson, J.D. et al. (1991) and Dorr, R.T. and Von Hoff, D.D., eds. (1994).
- antineoplastic agents are capable of suppressing both cellular and humoral immunity. Infections commonly lead to the death of patients with advanced cancer, and impaired immunity may contribute to such deaths. Chronic, delayed immunosuppression may also result from cancer chemotherapy.
- the major forms of neurotoxicity are arachnoiditis; myelopathy or encephalomyelopathy; chronic encephalopathies and the somnolence syndrome; acute encephalopathies; peripheral neuropathies; and acute cerebellar syndromes or ataxia.
- antineoplastic agents are mutagenic as well as teratogenic. Some, including procarbazine and the alkylating agents, are clearly carcinogenic. This carcinogenic potential is primarily seen as delayed acute leukemia in patients treated with polyfunctional alkylating agents and inhibitors of topoisomerase II, such as etoposide and the anthracycline antibiotics. Chemotherapy has also been associated with cases of delayed non-Hodgkin's lymphoma and solid tumors. The present invention will minimize these effects since the prodrug will only be activated within tumor cells. 6 The clinical usefulness of a chemotherapeutic agent may be severely limited by the emergence of malignant cells resistant to that drug.
- a number of cellular mechanisms are probably involved in drug resistance, e.g., altered metabolism of the drugs, impermeability of the cell to the active compound or accelerated drug elimination from the cell, altered specificity of an inhibited enzyme, increased production of a target molecule, increased repair of cytotoxic lesions, or the bypassing of an inhibited reaction by alternative biochemical pathways.
- resistance to one drug may confer resistance to other, biochemically distinct drugs.
- An alternative mechanism of resistance to cancer, chemotherapeutics occurs via the functional loss of tumor suppressor genes, especially p53, RB and pi 6.
- Amplification of the gene encoding dihydrofolate reductase is related to resistance to methotrexate, while overexpression/amplification of the gene encoding thymidylate synthase is related to resistance to treatment with 5- fluoropyrimidines. Smith (1995). Table 2 summarizes some prominent enzymes in resistance to biologic and chemotherapy.
- Tyrosine kinases TNF-alpha Hudziak, R.M. et al. PNAS 85:5102, 1988 Multidrug St ⁇ hlinger, M. et al. J. Steroid Biochem 49:39, resistance 1994
- Prodrugs are compounds that are toxicologically benign but which may be converted in vivo to therapeutically active products. In some cases, the activation occurs through the action of a non-endogenous enzyme delivered to the target cell by antibody ("ADEPT” or antibody-dependent enzyme prodrug therapy (U.S. Patent No. 4,975,278)) or gene targeting (“GDEPT” or gene 8 dependent enzyme-prodrug therapy (Melton, R.G. and Sherwood, R.F. (1996)).
- ADPT antibody-dependent enzyme prodrug therapy
- GDEPT gene 8 dependent enzyme-prodrug therapy
- This invention provides a method for identifying potential therapeutic agents by contacting a target or test cell with a candidate therapeutic agent or prodrug which is a selective substrate for a target enzyme in the cell.
- This new approach is named "ECTA", for Enzyme Catalyzed Therapeutic Agents.
- the target enzyme is an endogenous, intracellular enzyme which is overexpressed and confers resistance to biologic and chemotherapeutic agents.
- the activity of the enzyme has been greatly enhanced in a tumor cell as a result of loss of tumor suppressor function (Smith, K.A. et al. (1995) and Li, W. et al. (1995)) and or selection resulting from previous exposure to chemotherapy, (Melton, R.G. and Sherwood, R.F. (1996) and Lonn, U. et al. (1996)).
- the target enzyme is an expression product of an infectious agent in the cell.
- the cell is assayed for efficacy of the agent by noting if the agent caused a reduction in cellular proliferation or if the agent kills the cell.
- the prodrug kills the cell or inhibits the cellular proliferation by the release of a toxic byproduct from the prodrug by the target enzyme.
- one or more "target enzymes" can be used to activate the prodrug so that it releases the toxic byproduct.
- kits for use in assaying for new prodrugs having the characteristics described herein against target enzymes comprise the reagents and instructions necessary to complete the assay and analyze the results.
- This invention also provides methods and examples of molecules for selectively killing a pathological cell by contacting the cell with a prodrug that is a selective substrate for a target enzyme, e.g., an endogenous, intracellular enzyme as defined above.
- a target enzyme e.g., an endogenous, intracellular enzyme as defined above.
- the substrate is 9 specifically converted to a cellular toxin by the intracellular target enzyme.
- the product of an initial reaction is subsequently fully activated by a common cellular enzyme such as an acylase, phosphatase or other "housekeeping" enzyme
- this invention is a method for treating a pathology characterized by pathological, hype ⁇ roliferative cells in a subject by administering to the subject a prodrug that is a selective substrate for a target enzyme, and selectively converted by the enzyme to a cellular toxin in the hype ⁇ roliferative cell.
- the prodrugs of this invention may be used alone or in combination with other chemotherapeutics or alternative anti-cancer therapies such as radiation.
- a further aspect of this invention is the preparation of a medicament for use in treating a pathology characterized by pathological, hype ⁇ roliferative cells in a subject by administering to the subject a prodrug that is a selective substrate for a target enzyme, and selectively converted by the enzyme to a cellular toxin in the hype ⁇ roliferative cell.
- a still further aspect of this invention is a method for identifying the optimal therapeutic for a subject, by isolating cells overexpressing an endogeneous, intracellular enzyme and contacting the cells with at least one of the prodrugs of this invention, and then identifying which of the one or more prodrugs inhibits the proliferation or kills the cells, thereby indentifying the optimal therapeutic for the subject.
- Figure 1 shows the development of resistance to anti-cancer modalities in cells, and the consequences.
- Figure 2 schematically shows activation pathways of the prodrugs of this invention.
- Figure 3 schematically shows the High Throughput Screen for prodrugs activated by intracellular enzymes important in drug resistance.
- Figure 4 schematically shows how to find a lead human thymidylate synthase (TS) prodrug using TS-negative E. coli as the cell target.
- TS thymidylate synthase
- Figure 5 shows an example of how to use this screen to simultaneously optimize the prodrug for reactivity to 10 two target enzymes.
- Figure 6 shows one embodiment of a multi partitioned substrate for the generation of a thymidylate synthase (TS)-activatable prodrug.
- TS thymidylate synthase
- Box 1 represents a masked or absent phosphate group R 7 .
- R 7 When masked, it is a phosphoramidate or similar derivative that facilitates cell entry and is processed intracellularly to a monophosphate which can bind to TS. See Fries, K.M. et al. (1995).
- R 7 When absent, R 7 is a hydrogen atom and the prodrug is a substrate for cellular thymidine kinase (TK), which generates the requisite monophosphate in vivo.
- TK thymidine kinase
- Box 2 is a 2'-deoxyribofuranose group or other similar sugar, thio-sugar, carbocyclic, or acyclic group which connects the monophosphate to the pyrimidine ring in a manner that supports functional binding of the prodrug to TS and, when R 7 is a hydrogen atom, to TK.
- This group need not utilize an oxygen atom for attachment to the R 7 group of box 1.
- phosphonate analogs of sugar phosphates are acceptable.
- Box 3 represents a tether group, wherein n is an integer from 0 to 10, that is a mono- or polyunsaturated electron conduit acting to conduct electrons away from the pyrimidine ring and toward the leaving group R 4 when the prodrug is acted upon by TS.
- the tether group is comprised of 0 to 10 unsaturated moieties like acyclic vinyl, ethynyl, imine, or azo units or cyclic unsaturated, aromatic, or heteroaromatic ones that can be mixed and matched at will as long as their connectivity provides the requisite electron- conducting conduit.
- Box 4 represents a spacer unit X, wherein m is an integer from 0 to 1 , that connects the tether to the leaving group R 4 . If Box 3, n equals 0, then Box 4, m equals 1.
- X is a methylene (CH 2 ) unit, either bearing substituents or not. Additionally, though, X can be an oxygen, sulfur, nitrogen, or other atoms capable of forming at least two covalent bonds.
- X is absent (Box 4, m equals 0)
- the departure of the leaving group R 4 during the processing of the prodrug by TS leaves behind a pyrimidine nucleotide-based alkylating entity. See Barr, et al. (1983).
- Box 5 represents a leaving group R 4 that is released by the action of TS on the prodrug. It is itself a toxic antimetabolite or alkylating agent or is an entity that readily produces a toxic antimetabolite or alkylating agent in vivo.
- the leaving group 11 R 4 can depart as an active metabolite of the anticancer agents Tepa or Thiotepa, a phosphoramide mustard, N-acetylsphingosine (C 2 ceramide, a tumor suppressor lipid) or hydroxyurea (an inhibitor of ribonucleotide reductase) upon release by TS.
- the leaving group R can also be an ⁇ , ⁇ -dihalogenated ether, in which case it affords a carboxylic acid group when the ⁇ , ⁇ -dihalogenated alcohol released by TS undergoes hydrolysis.
- the leaving group R 4 can depart as a progenitor to fluoroacetate, fluorocitrate, malonic acid, methy lmalonic avid, or 3-nitroproprionic acid, all potent inhibitors of oxidative phosphorylation.
- Figure 7 shows TS Western blot of cell lines transfected by plasmid encoding neomycin resistance, with or without the HER-2 protooncogene.
- Lane (1) MCF7/HER2;
- MCF7/neo (2) MCF7/neo; (3) MDA-MB-435/HER2; (4) MDA-NM-435/neo; (5) BT-20/HER2; (6) BT-20/neo.
- Figure 8 shows reaction products of prodrug compounds with the enzyme thymidylate synthase.
- the invention is achieved by exploiting some of the key genomic and phenotypic changes intimately linked to resistance to biologic and chemotherapy of cancer cells.
- the invention provides a means for in vivo selectively inhibiting the growth and/or killing of cells which have undergone selection by exposure to cancer therapy (including biologic therapy such as tumor necrosis factor (TNF) or chemotherapy). (Refer to Table 2).
- cancer therapy including biologic therapy such as tumor necrosis factor (TNF) or chemotherapy.
- TNF tumor necrosis factor
- Table 2 certain enzymes which have been activated by mutation or gene amplification are resistant to initial or further therapy by the agent.
- this invention exploits the higher enzyme activity associated with therapy-resistant diseased cells and tissues versus normal cells and tissues and does not rely on inhibiting the enzyme.
- the tumor cells successfully treated by the prodrugs of this invention are characterized by enhanced target enzyme activity and therefore have a much higher potential to convert the prodrug to its toxic form than do normal cells which do not overexpress the target enzyme.
- target enzyme is used herein to define enzymes having one or more of the above noted characteristics. 12
- a cell includes a plurality of cells, including mixtures thereof.
- an “effective amount” is an amount sufficient to effect beneficial or desired results.
- An effective amount can be administered in one or more administrations, applications or dosages.
- the terms "host cells, "target cells” and “hype ⁇ roliferative cells” encompass cells characterized by the activation by genetic mutation or the endogenous overexpression of an intracellular enzyme.
- the overexpression of the enzyme is related to loss of tumor suppressor gene product function drug resistance or the genetic instability associated with a pathological phenotype.
- Enzymes activated or overexpressed and related to drug resistance include, but are not limited to thymidylate synthase (TS) (Lonn,
- resistance to one drug may confer resistance to other, biochemically distinct 13 drugs. While this application is specifically directed to cancer, a similar approach can be applied to enzymes encoded by human and animal pathogens, and in which the inhibitors have failed due to development of resistance.
- Amplification of certain genes is involved in resistance to chemotherapy.
- Amplification of dihydrofolate reductase (DHFR) is related to resistance to methotrexate while amplification of the gene encoding thymidylate synthase is related to resistance to tumor treatment with 5-fluoropyrimidines.
- Amplification of genes associated with drug resistance can be detected and monitored by a modified polymerase chain reaction (PCR) as described in Kashini-Sabet, et al. (1988), U.S. Patent No. 5,085,983, or the method described herein.
- Acquired drug resistance can be monitored by the detection of cytogenetic abnormalities, such as homogeneous chromosome staimng regions and double minute chromosomes both of which are associated with gene amplification.
- Alternative assays include direct or indirect enzyme activity assays and both of which are associated with gene amplification (e.g., Carreras & Santi (1995)); other methodologies (e.g. polymerase chain reaction, Houze, T.A. et al. (1997) or immunohistochemistry (Johnson,
- the target cell is characterized as having inactivated tumor suppressor function, e.g. loss or inactivation of retinoblastoma (RB) or p53, known to enhance expression of TS (Li, W. et al. (1995)) or DHFR (Bertino, et al. (1996) and Li, W. et al. (1995)).
- inactivated tumor suppressor function e.g. loss or inactivation of retinoblastoma (RB) or p53, known to enhance expression of TS (Li, W. et al. (1995)) or DHFR (Bertino, et al. (1996) and Li, W. et al. (1995)).
- the prodrugs of this invention are useful to treat or ameliorate any disease wherein the disease-associated enzyme is associated with drug resistance to a chemotherapeutic whether due to loss of tumor suppressor functionality, in vivo selection by chemotherapy or a combination.
- the enzyme is overexpressed, over- accumulated or activated in pathological cells versus normal cells, for example, the TS enzyme.
- the enzyme glutathione-S-transferase which has been shown to be occasionally elevated in some human tumors. Morgan, A.S. et al. (1998).
- the prodrugs of the subject invention are distinguishable on the basis that the target enzymes of this invention are commonly overexpressed, overaccumulated or activated in pathological cells versus normal cells. The most important principle which distinguishes the current invention from other approaches are: 14
- This invention describes the synthesis of substrates for enzymes like thymidylate synthase.
- the overexpressed enzyme will generate toxin, preferentially in diseased cells.
- Previous approaches have relied on an inhibitor.
- the inhibitors lead to amplified expression of the enzyme, and subsequent resistance to treatment (see, e.g., Lonn, U. et al. (1996).
- the current approach is also distinguishable from other "substrate-prodrug” approaches, e.g., the glutathione-S-transferase enzymes (see, e.g., Morgan, A.S. et al. (1998).
- the enzymes of the GST family are expressed at increased levels in response to toxic insult to the cell.
- the GST family of enzymes have overlapping substrate specificities, which makes it difficult to design a substrate reactive with only a single species of enzyme with elevated expression in a cancer cell (Morgan, A.S. et al. (1998)).
- each of the enzymes of the current invention e.g., thymidylate synthase, dihydrofolate reductase and thymidine kinase
- thymidylate synthase dihydrofolate reductase
- thymidine kinase a substrate for thymidylate synthase
- the gene encoding the target enzyme may have undergone mutation to give resistance to inhibitors, (Barbour, K.W. et al. (1992) and Dicken, A.P. et al. (1993)) but will still be capable of carrying out reaction with non- inhibitor substrate prodrugs.
- a further advantage of this approach is that loss of tumor suppressor function is critical to development of malignancy. The majority of tumor cells have lost one of the p53, RB or pi 6 tumor suppressor functions. Such a loss results in increased expression of resistance enzymes (e.g., Thymidylate synthase), independent of previous exposure to chemotherapy.
- the prodrugs described herein will be useful in treating early stages of malignancy, as well as disease previously treated with chemotherapy. Substrates for enzymes like GST require previous exposure of the tumor to chemotherapy in order to achieve sufficient overexpression to offer even the possibility of a modest therapeutic index.
- This invention provides a method for identifying agents which have therapeutic potential for the treatment of hype ⁇ roliferative or neoplastic disorders, e.g., cancer.
- the 15 method also identifies agents that inhibit the growth of cells or cell cycling of hype ⁇ roliferative cells, such as cancer cells.
- Other cells that are included are bacterial, yeast and parasitic cells which cause disease as a result of inappropriate proliferation in the patient.
- the agent is considered a potential therapeutic agent if cell proliferation, replication or cell cycling is reduced relative to the cells in a control sample. Most preferably, the cells are killed by the agent.
- the cells can be procaryotic (bacterial such as E. coli) or eucaryotic.
- the cells can be mammalian or non-mammalian cells, e.g., mouse cells, rat cells, human cells, fungi (e.g., yeast) or parasites (e.g., Pneumocystis or Leishmania) which cause disease.
- fungi e.g., yeast
- parasites e.g., Pneumocystis or Leishmania
- a "hype ⁇ roliferative cell” is intended to include cells that are de- differentiated, immortalized, neoplastic, malignant, metastatic or transformed. Examples of such cells include, but are not limited to a sarcoma cell, a leukemia cell, a carcinoma cell, or an adenocarcinoma cell.
- the cell can be a breast cancer cell, a hepatoma cell, a dectectable cancer cell, pancreatic carcinoma cell, an oesophageal carcinoma cell, a bladder cancer cell, an ovarian cancer cell, a skin cancer cell, a liver carcinoma cell, or a gastric cancer cell.
- the target cell can be resistant to a drug or compound used to prevent or kill a cell infected with an infectious agent which is resistant to coventional antibiotics. Infectious agents include bacteria, yeast and parasites, such as trypanosomes.
- target enzymes that are the subject matter of this invention are listed in Table 2 (above) or Table 3 (below). These enzymes are involved in resistance to chemotherapy, are endogeneously activated, overexpressed or over-accumulated in a cell characterized by resistance to cancer therapy and associated with a pathological or disease include, but are not limited to enzymes such as a member of the tyrosine kinase superfamily or an ATP-dependent MDR-associated protein, CAD, thymidylate synthase, dihydrofolate reductase, and ribonucleotide reductase. Table 3 provides a list of enzymes which may be targeted by this approach in infectious disease. Table 3 Enzymes Overexpressed in Infectious Disease, and which Contribute to Drug Resistance
- Enzyme Provides increased Resistance to:
- This invention also provides a quick and simple screening assay that will enable initial identification of compounds with at least some of the desired characteristics.
- Figure 3 This drawing describes how the assay is arranged and the materials necessary for its process.
- the assay requires two cell types, the first being a control cell in which the target enzyme is not expressed, or is expressed at a low level.
- the second cell type is the test cell, in which the target enzyme is expressed at a detectable level, e.g., a high level
- a procaryotic E. coli which does not endogenously express the target enzyme TS is a suitable host cell or target cell.
- the cell can have a control counte ⁇ art (lacking the target enzyme), or in a separate embodiment, a counte ⁇ art genetically modified to differentially express the target enzyme, or enzymes (containing the appropriate species of target enzyme). More than one species of enzyme can be used to separately transduce separate host cells, so that the effect of the candidate drug on a target enzyme can be 17 simultaneously compared to its effect on another enzyme or a corresponding enzyme from another species.
- cells containing the desired genetic deficiencies may be obtained from Cold Spring Harbor, the Agricultural Research Service Culture Collection, or the American Type Culture Collection.
- the appropriate strains can also be prepared by inserting into the cell a gene coding for the target enzyme using standard techniques as described in Miller (1992), Sambrook, et al. (1989), and Spector et al. (1998). Growth assays can be performed by standard methods as described by Miller (1992), Sugarman et al. (1985) and Spector et al. (1998). It should be understood by those skilled in the art that the screen shown in Figure 3 can be applied broadly for the discovery of antibiotics. For example, thymidylate synthase from yeast could be substituted for that of E. coli in Figure 4.
- Enzymatic assays also can be performed as reviewed by Carreras, C.W. and Santi, D.N. (1995) using detectable labeled substituents, e.g. tritium labeled substituents.
- detectable labeled substituents e.g. tritium labeled substituents.
- a possible advantage of the "two enzyme" system is that the requirement for activation by two enzymes preferentially overexpressed in tumor cells will provide increased safety for normal cells.
- a tumor sample is removed from the patient and the cells are assayed for the level of expression of the enzyme of interest. If the expression is above that expressed in normal cells so that a toxic amount of the prodrug would cause administered without undesirable side effects, then the tumor or cells are determined to be remedially treated and thus, the patient is suitable for the therapy of this invention.
- the target enzyme is expressed at least about 2 times and preferably about 3 times higher than normal cells, the patient is a suitable subject for the therapy method of this invention.
- Therapeutic amounts can be empirically determined and will vary with the pathology being treated, the subject being treated and the toxicity of the converted prodrug or cellular toxin.
- Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets or tablets, each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution or suspension in an aqueous or non-aqueous liquid; or as an oil-in-water liquid emulsion or a 24 water-in-oil liquid emulsion.
- the active ingredient may also be presented a bolus, electuary or paste.
- uracil base only
- uridine base and sugar
- the gene encoding TS is introduced to target cells by electroporation, transformation or transfection procedures. Sambrook, et al. (1989). Alternatively, the gene is inserted into an appropriate expression vector by methods well known in the art, e.g., as described in Carreras, C.W. and Santi, D.V. (1995), Miller (1992) and Spector et al. (1998). The expression vector inserts the TS gene into the cells. The cells are then grown under conditions which favor the expression and production of TS protein.
- Human gastric cancer cell lines MKN-74, MKN-45, MKN-28 and KATO-III can be used in the assay described above to identify potential therapeutic agents which are selective substrates for TS.
- MKN-74 and MKN-45 are established from well and poorly differentiated adenocarcinomas, respectively. These cell lines and culture conditions are described in Osaki, M. et al. (1997) and references cited therein.
- tumor cell lines such as those described by Copur, S. et al. (1995), which have been selected by 5-FU to overexpress thymidylate synthase may be used.
- the present invention involves two classes of compounds activated by TS, each a derivative of 5-substituted deoxyuridine monophosphate or a protected 5-substituted uracil or deoxyuridine monophosphate.
- Protected 5-substituted deoxyuridine monophosphate derivatives are those in which the phosphate moiety has been blocked through the attachment of suitable chemical protecting groups. Protection of 5-substituted deoxyuridine monophosphate derivatives can improve solubility, facilitate cellular penetration, facilitate passage across the blood-brain barrier, and prevent action of cellular or extracellular phosphatases, which might otherwise result in loss of the phosphate group.
- sugar group derivatives include “deoxy”, “keto”, and “dehydro” derivatives as well as substituted derivatives.
- thio sugar groups include the sulfur analogs of the above sugar groups, in which the ring oxygen has been replaced with a sulfur atom.
- carbocyclic groups include C 4 carbocyclic groups, C 5 carbocyclic groups, and C 6 carbocyclic groups which may further have one or more subsituents, such as -OH groups.
- Q is a ⁇ -D-ribofuranosyl group of the formula: 36
- further aspects include: wherein R 2 and R 3 are H; wherein R 2 is OH and
- n is an integer from 0 to 10; wherein A is a phosphoramide derivative, or a compound of the formula: o P— N(CH 2 CH 2 CI) 2
- R 7 is selected from the group consisting of H, phosphoryl, phosphoramidate and derivatives thereof, and wherein R 2 and R 3 are the same or different and are independently -H or -OH. In one embodiment, R 7 is not H.
- the candidate therapeutic agent is a compound of the formula:
- R 2'-deoxy-5-uridyl
- m is 0 or 1
- n is an integer from 0 to 10.
- the compounds can be in any of their enantiomeric, diasteriomeric, or stereoisomeric forms, including, for example, D- or L-forms, and can be in any stereochemical configuration, including, for example, ⁇ - or ⁇ -anomeric form.
- C 5 -modification of pyrimidine nucleosides and nucleotides is the formation of C5-trans-styryl derivatives by treatment of unprotected nucleotide with mercuric acetate followed by addition of styrene or ring-substituted styrenes in the presence of Li 2 PdCl 4 .
- Bigge, et al. (1980) Pyrimidine deoxyribonucleoside triphosphates were derivatized with mercury at the 5 position of the pyrimidine ring by treatment with mercuric acetate in acetate buffer at 50° for 3 hours. Dale, et al. (1973).
- Such treatment would also be expected to be effective for modification of monophosphates; alternatively, a modified triphosphate could be converted enzymatically to a modified monophosphate, for example, by controlled treatment with alkaline phosphatase followed by purification of monophosphate.
- Other moieties, organic or nonorganic, with molecular properties similar 39 to mercury but with preferred pharmacological properties could be substituted.
- 5-position substituent is the halovinyl group, e.g. E-5-(2-bromovinyl)-2'- deoxyuridylate. Barr, P.J. et al. (1983).
- This compound is synthesized as follows as described in the experimental section.
- 5-bromodeoxyuridine, 5-iododeoxyuridine, and their monophosphate derivatives are available commercially from Glen Research, Sterling, VA (USA), Sigma- Aldrich Co ⁇ oration, St. Louis, MO (USA), Moravek Biochemicals, Inc., Brea, CA (USA), ICN, Costa Mesa, CA (USA) and New England Nuclear, Boston, MA (USA).
- 5-bromodeoxyuridine and 5-iododeoxyuridine can be converted to their monophosphates either chemically or enzymatically, though the action of a kinase enzyme using commercial available reagents from Glen Research, Sterling, VA (USA) and ICN, Costa Mesa, CA (USA). These halogen derivatives could be combined with other substituents to create novel and more potent antimetabolites.
- the prodrug contacted with the cell overexpressing thymidylate synthase is an L or D isomer of a compound of the formula:
- R 1 is a moiety of the formula:
- R 2 is or contains a divalent electron conduit moiety.
- R 2 is or contains a mono- or polyunsaturated electron conduit acting to conduct electrons away from the pyrimidine ring and toward the leaving group R 1 .
- R 2 is selected from the group consisting of: an unsaturated hydrocarbyl group; an aromatic hydrocarbyl group comprising one or more unsaturated hydrocarbyl groups; and, a heteroaromatic group comprising one or more unsaturated hydrocarbyl groups.
- R 2 and R 3 taken together form a structure selected from the group consisting of:
- R 2 is an aromatic hydrocarbyl group having a structure selected from the group consisting of:
- R 2 is a heteroaromatic group having a structure selected from the group consisting of:
- J is a heteroatom, such as -O-, -S-, or -Se-, or a heteroatom group, such as - NH- or -NR ⁇ -, where ⁇ is a linear or branched alkyl having 1 to 10 carbon atoms or a cycloalkyl group having 3 to 10 carbon atoms.
- R 3 is a divalent spacer moiety, also referred to as a spacer unit.
- R 3 is a divalent spacer moiety having a structure selected from the group consisting of:
- R 5 is the same or different and is independently a linear or branched alkyl group having from 1 to 10 carbon atoms, or a cycloalkyl group having from 3 to 10 carbon atoms.
- R 3 is a divalent spacer moiety having a structure selected from the group consisting of:
- n is an integer from 0 to 10 and, m is 0 or 1. In one embodiment, n is an integer from 0 to 10 and, m is 1. In one embodiment, n is 0 and m is 0. In one embodiment, when R 7 is -H, then n is not zero. In one embodiment, when R 7 is -
- R 7 is -H, then n is not zero and m is not zero.
- R 4 is not a halogen (i.e., -F, -Cl, -Br, -I).
- R 4 is not a halogen (i.e., -F, -Cl, -Br, - I).
- R 4 is not a halogen (i.e., -F, -Cl, -Br, - I).
- R 4 is not a halogen (i.e., -F, -Cl, -Br, -I).
- R 4 is a toxophore moiety.
- toxophore shall mean a moiety which is or contains a leaving group which is a chemical entity that has a molecular dimension and electrophilicity compatible with extraction from the pyrimidine ring by thymidylate synthase, and which upon release from the pyrimidine ring by thymidylate synthase, has the ability to inhibit the proliferation of the cell or kill the cell.
- the toxophore is or contains a leaving group that is activated or released by an intracellular enzyme overexpressed in the cell.
- R 4 is or contains a group having a structure selected from the group consisting of:
- X is -Cl, -Br, -I, or other potent leaving group (including, but not limited to, -CN. -OCN, and -SCN); Y is the same or different, and is independently -H or -F; and Z is the same or different and is independently -O- or -S-.
- R 4 is or contains a group having a structure selected from the group consisting of:
- R 4 is or contains a group having a structure selected from the group consisting of:
- R 4 is or contains a group having the structure:
- R 4 is or contains a group having the structure:
- R 4 is or contains a group having a structure selected from the group consisting of:
- R 4 is or contains a group having the structure:
- R 4 is or contains a group having the structure:
- R 4 is or contains a group having the structure:
- R 4 is or contains a chemical entity selected from the group consisting of: -Br, -I, -O-alkyl, -O-aryl, O-heteroaryl, -S-alkyl, -S-aryl, -S-heteroaryl, -CN, -OCN, -SCN, -NH 2 , -NH-alkyl, -N(alkyl) 2 , -NHCHO, -NHOH, -NHO-alkyl, NH,CONHO-, NHNH 2 , -N 3 , and a derivative of cis-platin, such as:
- Q is or contains a sugar moiety or a similar moiety which supports functional binding of the prodrug to the enzyme, e.g., TS or TK.
- Q is selected from the group consisting of:
- R 7 is hydrogen , a phosphate group, a phosphoramidate group, or other phosphorus containing group.
- Q is:
- Q is:
- R 7 is hydrogen. In one embodiment, R 7 is not hydrogen. In one embodiment, R 7 is a phosphate group or a phosphoramidate group. In one embodiment, R 7 is a phosphate group or a phosphoramidate group. In one embodiment, R 7 is a phosphoramidate group.
- R 7 is a phosphoramidate group derived from an amino acid, including, for example, the twenty naturally occuring amino acids. In one embodiment, R 7 is a phosphoramidate group derived from alanine. In one embodiment, R 7 is or contains a group having the structure:
- R 7 is a phosphoramidate group derived from tryptophan. In one embodiment, R 7 is or contains a group having the structure:
- R 7 is a phosphate group. In one embodiment, R 7 is or contains a group having a structure selected from the group consisting of:
- R 7 is or contains a group having a structure selected from the group consisting of (where R is an aromatic substituent):
- the R 7 forms a cyclic group within Q.
- DMTr 4,4'-dimethoxytrityl
- Boc is t-butyloxycarbonyl
- DCC is 1,3-dicyclohexylcarbodiimide
- 4-DMAP is 4- dimethy laminopyridine
- the compound may be in any enantiomeric, diasteriomeric, or stereoisomeric form, including, D-form, L-form, ⁇ -anomeric form, and ⁇ -anomeric form.
- the compound may be in a salt form, or in a protected or prodrug form, or a combination thereof, for example, as a salt, an ether, or an ester.
- the prodrug is a compound of the formula:
- the prodrug is a compound of the formula:
- the prodrug is a compound of the formula:
- the prodrug is a compound of the formula:
- the prodrug is a compound of the formula:
- the above structures are further modified to possess thiophosphodiaziridine instead of phosphodiaziridine groups, using the methods described below.
- the structures at the 5-position of uracil are referred to as the tethers because they connect the proposed leaving group (toxophore) to the heterocycle.
- a negative charge is conducted from the 6-position of uracil into the tether.
- This mechanism has been described for the 5'- monophosphorylated versions of (E)-5-(bromovinyl)-2'-deoxyuridine (BVDU) by Barr et al. (1983) and of (E)-5 -(3,3,3 -trifluoro-l-propenyl)-2' -deoxyuridine (TFPe-dUrd) by Wataya et al. (1979), Santi (1980), and Bergstrom et al. (1984).
- the tether "spacer" between the toxin and dNMP must be unsaturated so that it can conduct the toxin-labilizing negative charge supplied by the TS-Cys-sulfhydryl attack.
- the vinyl, allyl, and propargyl units are simple, small, and readily accessible synthetically.
- the vinyl and allyl units have the advantage that they can be prepared in either of two non-interconvertible geometric isomeric forms. Thus, they can be used as "probes" of prodrug accommodation by the TS active site.
- the propargyl unit has the advantage of being cylindrically symmetrical, so that TS-catalyzed toxin release from this type of tether does 51 not depend upon its orientation with respect to dUMP's uracil ring, as is the case with the vinyl and allyl molecules.
- the Spacer (e.g., -CH 2 -)
- the activated nucleoside rearranges to give toxic Released toxophore attacks product (e.g., modified dNMP) intracellular target
- toxic Released toxophore attacks product e.g., modified dNMP
- EdUMP and 5 -(3 -hydroxy-l-propynyl)-2' deoxyuridine 5 '-monophosphate (HOPdUMP) 52 with TS (Barr et al. 1981, Ban and Robins 1983).
- EdUMP is a potent inhibitor of TS (Ki
- esters derived from the propargylic or allylic alcohols the carboxylic acid moiety of toxophores like 3-nitroproprionic acid is "masked" by attaching them to the alcohol as ⁇ , ⁇ -difluoro ethers.
- a propargyl or allyl thioester tether is first prepared by treating the normal ester with Lawesson's reagent (Cava and Levinson (1985)), and then converting the thioester to the ⁇ , ⁇ -difluoro ether in an established fashion with tetrabutylammonium dihydrogentrifluoride (Kuroboshi and Hiyama (1991) and (1994)).
- the ⁇ , ⁇ -difluoro ether-based tether is then condensed with a protected 5-iodo-2' -deoxyuridine as usual.
- a protected 5-iodo-2' -deoxyuridine as usual.
- the masked toxophore will be stable until TS releases it as a latent reactive acvl fluoride.
- the ensuing rapid hydrolysis will generate the carboxylic acid-based toxophore in situ.
- p-Nitrophenyl ether derivatives of the CS propargylic and (E) and (Z)-allylic alcohol-equipped 2'-deoxyuridines provide good reagents for in vitro TS enzyme assays, because the spectrophotometric, kinetic determination of released p-nitrophenol will identify those tethered dUMP platforms that bind to TS in a manner that permits facile catalytic release of a leaving group from the end of the tether.
- the needed p-nitrophenyl ethers will be obtained either directly from the alcohols by a base-catalyzed condensation with 4-fluoronitrobenzene or de novo by a Heck coupling of an appropriate p-nitrophenyl propargyl or allyl ether.
- the 5 '-monophosphates needed for the TS assay are generated from the nucleosides either enzymatically or chemically according to a well-established regioselective 5'-monophosphorylation protocol (Imai et al. (1969)).
- preferential phosphorylation of the prodrug in tumor cells is facilitated as a result of 57 overexpression of thymidine kinase in most tumor cells as a result of tumor suppressor gene loss (Hengstschlager et al. (1996)).
- preferential activation of the phosphorylated prodrug or phosphoramidate derivative will occur in tumor cells because of the overexpression of thymidylate synthase accompanying tumor suppressor loss (Li, W. et al. (1995)) and chemotherapy (Peters, G.J. et al. (1995)).
- Combinatorial chemistry targeting the 5-position of the uridine ring (from which the cell toxin may be generated), or the 5 '-position of the pentose sugar (to facilitate binding to thymidylate synthase), will greatly expedite the discovery of lead compounds by optimizing the structure of the leaving group (cell toxin) from the uridine ring, as well as facilitating optimization of a phosphorylation competent group at the 5 '-position of the pentose sugar.
- the chemical entity being tested will be generated by either a single site directed chemistry (Figure 4), or simultaneously at two sites on the molecule ( Figure 5).
- Figure 4 a single site directed chemistry
- Figure 5 a powerful system for discovery of prodrugs targeting thymidylate synthase has been developed.
- the combinatorial chemistry approach used will be similar to that described in Lam, K.S.
- prodrugs that provide toxic leaving groups are shown in Figure " 6.
- the uridine substrates shown in Figure 6 take advantage of phosphoramidase (present in all cells) and elevated thymidylate synthase (TS) in tumor cells.
- TS thymidylate synthase
- the screen shown in Figure 3 can be applied broadly for the discovery of antibiotics.
- thymidylate synthase from yeast could be substituted for that of E. coli in Figure 4. This would allow the discovery of specific antifungal antibiotics targeting yeast related pathogens.
- other enzymes can be subjected to this treatment.
- prodrugs which target specifically the dihydrofolate reductase activity of infectious agents, like Pneumocystis carnii, could be selected. These agents will be selected for specificity for the target enzyme, and can be shown not to activate the enzyme of the natural host by employing the screening assay described in Figure 3.
- the control cellular constructs would contain the co ⁇ esponding normal human enzyme, in order to show lack of toxicity when only the normal human enzyme is present.
- the drugs or agents of the invention or as described herein can be made more permeable, for example across cell membranes and across the blood-brain barrier, by means of various chemical modifications. These would include attachment, to their phosphate moiety, of various functional groups which improve membrane permeability. Such functional groups include, but are not limited to, those described in U.S. Patent Nos.
- Salts of the prodrugs of the present invention may be derived from inorganic or organic acids and bases.
- acids include hydrochloric, hydrobromic, sulfuric, nitric, perchloric, fumaric, maleic, phosphoric, glycollic, lactic, salicyclic, succinic, toluene-p-sulfonic, tartaric, acetic, citric, methanesulfonic, ethanesulfonic, formic, benzoic, malonic, naphthalene-2-sulfonic and benzenesulfonic acids.
- acids such as oxalic
- bases include alkali metal (e.g., sodium) hydroxides, alkaline earth metal (e.g., magnesium) hydroxides, ammonia, and compounds of formula NW 4 + , wherein W is
- salts include: acetate, adipate, alginate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, citrate, camphorate, camphorsulfonate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, fumarate, flucoheptanoate, glycerophosphate, hemisulfate, heptanoate, hexanoate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesulfonate, lactate, maleate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, oxalate, palmoate, pectinate, persulfate, 59 phenylproprionate, picrate, pivalate, propionate, succinate, tartrate, thiocyanate, tosylate
- salts of the compounds of the present invention will be pharmaceutically acceptable.
- salts of acids and bases which are non- pharmaceutically acceptable may also find use, for example, in the preparation or purification of a pharmaceutically acceptable compound.
- the phosphate esters may be further esterified by, for example, a C,. 20 alcohol or reactive derivative thereof, or by a 2,3- di-(C 6 . 24 )acyl glycerol.
- any alkyl moiety present advantageously-contains from 1 to 18 carbon atoms, particularly from 1 to 6 carbon atoms, more particularly from 1 to 4 carbon atoms.
- Any cycloalkyl moiety present in such esters advantageously contains from 3 to 6 carbon atoms.
- Any aryl moiety present in such esters advantageously comprises a phenyl group.
- lyxo-furanosyl prodrug derivatives of the present invention include, for example, those with chemically protected hydroxyl groups (e.g., with O-acetyl groups), such as 2'- ⁇ 9-acetyl-lyxo-furanosyl; 3'-O- acetyl-lyxo-furanosyl; 5'-0-acetyl-lyxo-furanosyl; 2',3'-di- ⁇ -acetyl-lyxo-furanosyl and 2',3',5'-tri-0-acetyl-lyxo-furanosyl.
- chemically protected hydroxyl groups e.g., with O-acetyl groups
- Ethers of the compounds of the present invention include methyl, ethyl, propyl, butyl, isobutyl, and sec-butyl ethers.
- the substrate may not be chemically related to pyrimidines or folates, but rather synthesized based upon known parameters of rational drug design. See Dunn, W.J. et al. (1996). 60 Chemical assays for products, for example, where a reaction product is an antimetabolite of the bromo vinyl-derivatives of dUMP, are described in the Examples provided below or by Ban, P.J. et al. (1983).
- Another aspect of the present invention pertains to the methods for screening for a therapeutic agent, comprising contacting a target cell with a prodrug compound this invention.
- the prodrug is a compound to the group of Class II, as described below, wherein R 4 is:
- a phosphoramidate derivative of 5-fluoro-2'-deoxyuridine (5-FUdR), and (E)-5-(2- bromovinyl)-2'-deoxyuridine (BVDU) and a phosphoramidate derivative of BVDU were synthisized.
- BVDU was prepared from 2'-deoxyuridine according to a literature procedure (Dyer, et al. (1991)). According to standard methods, it was first protected at the 05' position with a dimethoxytrityl (DMTr) group and then at the 03' position with a tertbutyldimethylsilyl (TBDMS) group, and then the 5'-O-DMTr group was removed by treatment with acid (see below). 61
- DMTr dimethoxytrityl
- TDMS tertbutyldimethylsilyl
- TBDMS-protected BVDU-PA TBDMS-protected BVDU-PA.
- TBAF tetrabutylammonium fluoride
- BVDU-PA To synthesize (E)-5-(Bromovinyl)-2'-deoxy-5'-uridyl phenyl L- alaninylphosphoramidate (BVDU-PA), a solution of BVDU (420 mg, 1.26 mmol) in 2 mL of anhydrous DMF under argon was treated with imidazole (103 mg, 1.51 mmol) and then dropwise with phenyl L-methoxyalaninyl phosphorochloridate (350 mg, 1.26 mmol
- 5-Fluoro-2'-deoxy-5'-uridyl phenyl L-alaninylphosphoramidate (5FUdR-PA) was obtained in a purity of at least 98%, by TLC and ⁇ NMR.
- 5FUdR-PA 5-Fluoro-2'-deoxy-5'-uridyl phenyl L-alaninylphosphoramidate
- one aspect of the invention pertains to methods of preparing a 2'-deoxy, 3'-hydroxy, 5 '-phosphoramidate of a furanosyl nucleoside, which methods involve reacting an unprotected furanosyl nucleoside which is 2'-deoxy, 3'-hydroxy, and 5'- hydroxy, with a phosphochloridate in the presence of an HCl scavenger.
- the phosphochloridate comprises a phosphorus substituent which is derived from an amino acid, such as alanine.
- the phosphochloridate is phenyl- 65 L-methoxyalanine phosphorochloridate.
- the present invention pertains to a method of forming a compound of the formula: . O
- Base denotes a nucleic acid base (such as uracil, thymine, cytosine, adenine, or guanine, but preferably uracil, or a derivative thereof); which method comprises the step of reacting a compound of the formula:
- the furanosyl nucleoside is a uridine nucleoside. In another embodiment, the furanosyl nucleoside is a uridine ribofuranosyl nucleoside. In another embodiment, the furanosyl nucleoside is a uridine ⁇ -D-ribofuranosyl nucleoside.
- the present invention pertains to a method of forming a compound of the formula: 66
- R 1 is a substituent (including, for example, those described above for R 1 ); which method comprises the step of reacting a compound of the formula:
- the reaction is performed in the absence of base, but in the presence of an HCl scavenger, such as imidazole.
- an HCl scavenger such as imidazole.
- the reaction is carried out in a non-aqueous medium.
- the reaction is carried out in a non-aqueous medium comprising anhydrous dimethylformamide (DMF).
- H630R10 Copur, et al. (1995)
- CCD18co normal colon epithelial cells
- the H630P (Copur et al. (1995)) cell line was selected for resistance to lO ⁇ M 5-FU to give rise to H630R10.
- a normal colon epithelial cell type CCDl ⁇ co available from the ATCC was used for comparison with H630R10 for sensitivity to test compounds.
- Cell lines expressing pl85 - HER2 or neomycin marker alone were prepared as described by Pegram et al. (1997) shows that H630R and H630R10 expresses 10 fold increased thymidylate synthase enzyme as compared with CCD 18co as determined by Western blot analysis.
- test compounds The ability of the test compounds to block proliferation of cells was determined by the crystal violet procedure (Sugarman et al. (1985) and Antelman et al. (1995)).
- thymidylate synthase transcripts in different cell lines were quantified by using RT-PCR.
- Oligonucleotide primers for amplification of the human thymidylate synthase and B-actin were designed as follows: Thymidylate synthase sense primer 5'-GGGCAGATCCAACACATCC-3' (corresponding to bases 208-226 of thymidylate synthase cDNA sequence, Genbank Accession No.
- antisense primer 5'-GGTCAACTCCCTGTCCTGAA-3' (corresponding to bases 564-583)
- ⁇ -actin sense primer 5'-GCCAACACAGTGCTGTCTG-3' co ⁇ esponding to bases 2643-2661 of ⁇ -actin gene sequence, Genbank accession no. Ml 0277
- antisense primer 5'- CTCCTGCTTGCTGATCCAC-3' (corresponding to bases 2937-2955).
- RNAs were isolated from CCD18co and H630R10 cells, using Rneasy mini kit (obtained from Qiagen, Valencia, CA). To monitor for possible DNA contamination, the primers for amplification of ⁇ -actin were designed to span the exon4/intron5/exon5 junction. Genomic DNA template leads to a 313 bp ⁇ -actin fragment, and cDNA template generates a 210 bp product.
- Reverse transcription reactions were performed, using Superscript preamplification system (Gibco/BRL, Gaithersburg, MD). 3 ⁇ g total RNA was applied in a volume of 20 ⁇ l buffer to conduct reverse transcription reaction, followed manufacturer's protocol. PCR reactions were performed in a volume of 48 ⁇ l, containing 3 ⁇ l of cDNA mixture from reverse transcription reaction, 3 mM MgCl 2 , 50 mM KCl, 20 mM Tris-Cl, pH 8.4, 0.2 mM of each dNTP, 0.4 ⁇ M of thymidylate synthase sense and antisense primers and 3 units of Tag DNA polymerase (obtained from Promega, Madison, WI).
- reaction mixtures were incubated at 94°C for 3 min, followed by 10 cycles of 1 min incubation at 94°C, 1 min incubation at 58°C , and then 1 min incubation at 72°C After 10 cycles, human ⁇ -actin primers in 2 ⁇ l were added to achieve a final concentration of 0.2 ⁇ M, bringing the final reaction volume to 50 ⁇ l.
- PCR reaction was continued to a total of 28 cycles, followed by a 7 min incubation at 72°C 69 5 ⁇ L of PCR products were resolved by electrophoresis in 2% agarose gel, followed by staining with SYBR Gold nucleic acid gel stain (obtained from Molecular Probes, Eugene, OR). The DNA bands co ⁇ esponding to thymidylate synthase were quantified and normalized to that of ⁇ -actin by Molecular Dynamics Storm.
- Northern blots were obtained from Invitrogen (Carlsbad, CA) and hybridized to a cloned TS cDNA probe. Hybridization signals were normalized vs. ribosomal protein S9 as a housekeeping transcript. A tumor was considered to overexpress TS mRNA if the normalized signal was enhanced at least 2-fold as compared to the normal tissue control.
- An E. coli plasmid expression vector for human thymidylate synthase (TS) was constructed using a modified human TS cDNA in pGCHTS. This clone, obtained from Dr. Dan Santi, contains the complete open reading frame for human TS.
- the complete human TS ORF was subcloned into the T7 promoter expression vector pET28a (Novagen Inc.).
- the resulting plasmid vector encodes the synthesis of human TS as a recombinant fusion protein with a six histidine followed by a thrombin cleavage site.
- This fusion protein adds a total of 20 extra amino acids to the amino terminus of human TS.
- the human TS expression vector was introduced into the E. coli strain BL21(DE3), a strain with the T7 RNA polymerase gene inserted into the chromosome under the control of the lac operator.
- the human TS-poly-His fusion protein was purified using a metal chelating affinity resin (Novagen Inc.). Protein purification was followed by electrophoresis on ⁇ 0% SDS polyacrylamide gels. Protein concentrations were determined using the Pierce BCA protein assay. Approximately 10 mg of human TS fusion protein was recovered from a 500 ml culture of E. coli. When visualized with silver stain only the band co ⁇ esponding to human TS was apparent. The identity of the human TS band was confirmed by performing a Western blot with the anti-TS monoclonal antibody TS106 (NeoMarkers). 70 Thymidylate Synthase Enzyme Activity Assay
- TS activity was measured using the spectrophotometric assay of Wahba and Friedkin (1961). In this assay, TS activity is monitored by measuring the increase in absorbency at 340 nm that occurs when the co-factor 5,10 methylene tetrahydrofolte is oxidized to dihydrofolate as dUTP is converted to dTTP. Enzyme prepared by this method has a specific activity of 0.5 - 0.65 units/mg protein. One unit is defined as the production of one micromole of dTNP per minute. This value is similar to that reported by Pedersen- Lane et al. (1997).
- HER2 Protooncogene Expression Increases TS Levels in Tumor Cells
- TS expression levels in neomycin and HER2/neu-transfected human breast carcinoma cells were assessed using immunoblotting techniques. The results (see Figure
- MCF7/HER2 and MCF7/neo cell lines are target cells for assays of this invention because induction of TS is most pronounced (about 20- fold) in these cell types.
- the fluoropyrimidine, 5-FUdR inhibits cell growth via mechanisms similar to 5- FU. This includes cytotoxic effects resulting from inco ⁇ oration into nucleic acid, as well as inhibition of TS enzyme activity (Goodman and Gilman (1996)). It is therefore expected that cells expressing higher levels of TS will often be more resistant (have a higher IC 50 ) than cells which express lower amounts of the enzyme. Table 6 (Line 1) demonstrates that this is the result obtained. Furthermore, as expected from earlier work, BVDU has little activity in the assay (Table 6; line 2).
- BVDU-PA (Table 6, line 3) has more than 10-fold greater activity on the higher expressing H630R10 cell line than on CCDl ⁇ co cells.
- the prodrug substrate, BVDU-PA reverses this relationship.
- TS Prodrug BVDU-PA has Greater Activity on a Thymidylate Synthase Overexpressing Tumor Cell Line than on Normal Cells (1, 2).
- An additional assay of this invention requires candidate drugs to be screened in reaction mixtures containing human thymidylate synthase with and without N5N10- methylenetetrahydrofolate, and the candidate prodrug.
- the leaving group of the candidate prodrug e.g., at the pyrimidine 5 position
- the control substrate is similarly labeled (e.g. 5- 3 H) dUMP. under the same reaction conditions.
- the assays are done similarly to the description provided in Carreras, C.W. and Santi, D.V. (1995), and references cited therein.
- the human thymidine synthase can be purified from E. coli containing the expressed human thymidylate synthase. See Davisson, V.J. et al. (1989) and Davisson, V.J. et al. (1994). This approach provides a scaleable assay capable of screening large numbers of candidate compounds.
- TS prodrug metabolism It is important to determine the intracellular products of TS prodrug metabolism in order to substantiate proposed mechanism of activation and action and to define agents that are candidate thereapeutics.
- One acceptable view of intracellular metabolism of aryl phosphodiester amidates involves enzymatic conversion into a carboxylic acid, intramolecular rearrangement of the phosphomonoester amidate into to a 5'- monophosphoryl nucleoside (Valette et al. (1996)). However, this mechanism is unlikely to describe intracellular processing of all phosphoramidate-based pronucleotides.
- This assay determines the specific activities of future TS enzyme preparations, to assure the activity of such preparations over time, and to determine whether compounds screened for suitable activity do inactivate the TS enzyme under in vitro reaction conditions.
- reaction conditions similar to those employed with the TS activity assay are used to generate reaction products in vitro. These reaction products will are then analyzed by GC-mass spectrometry to identify the actual molecules formed.
- MCF7/HER-2-transfected human breast carcinoma cells as well as other HER-2/neu-transfected cell lines demonstrate an increase in TS expression (Pegram et al. (1997)).
- these lines are suitable for testing differential response to prodrug.
- the differential responses are first confirmed in vitro for MCF7/NEO and MCF7/HER-2 cells to 5-FUdR and BVDU-PA using the same in vitro experimental approach described above.
- the HT1080 tumor cell lines from Dr. Bertino (Banerjee et al. (1998)) are similarly characterized.
- this model formed the basis for the preliminary in vivo analysis of the drug Herceptin® which has recently been approved by the US Food and Drug Administration for the treatment of metastatic breast cancer (Pegram, et al. (1998)).
- the colon tumor cell lines (HT1080/NEO and HT1080/E2F1.1) have demonstrated tumorigenicity (Banerjee et al. (1998)).
- Ras-transformed NIH 3T3 cell lines are transplanted subcutaneously into immunodeficient mice.
- Initial therapy may be direct intratumoral injection.
- the expected result is that increased level of expression of human TS or a target enzyme leads to enhanced antitumor activity by the drug candidates.
- Similar studies are performed with human tumors expressing increasing levels of human TS or a target enzyme, and demonstrating that efficacy in response to drug correlates with their level of human TS expression or target enzyme.
- experiments are be performed as above except the drug will be administered intravenously into the animals to address issues related to efficacy, toxicity and pharmacobiology of the drug candidates.
- control animals will receive an effective amount of a compound identified above under the section entitled "Prodrugs," supra.
- Test cells with defined expression of TS are injected subcutaneously at 5 X 10 6 /site in the flank region of 4-6 week old (20-25 gram) CD-I (nu/nu) athymic mice (Charles River Laboratories, Wilmington, MA) as described
- mice Eight mice (four animals per cage) are used for each treatment group (vehicle control solution or prodrug compounds). Two xenografts are established per mouse both reduce the number of animals needed for each experiment and improve the statistical power to detect differences in response between groups. Individual mice are identified by tagging the ears so that xenograft trajectories are calculated for individual mice in addition to the analysis of group means. All mice used in the MCF-7 breast xenograft model are female and, prior to cell injection, are primed with 17B-estradiol (Innovative Research of America, Toledo, OH) applied subcutaneously in a biodegradable carrier binder (1.7 mg estradiol/pellet) to promote tumor cell growth.
- 17B-estradiol Innovative Research of America, Toledo, OH
- Prospective tumor volumes calculated as (width 2 X length)/2 are monitored twice weekly by serial micrometer measurements by a single blinded observer. Animals are assigned to each treatment group such that the mean starting tumor volumes will be the same in each group. Statistical tests are performed (single-factor ANOVA) to assure uniformity in starting tumor volumes between treatment groups.
- the prodrugs or isovolumetric vehicle control solution are administered by single I.P. injection in the first set of experiments. If efficacy is identified in the initial experiments, additional studies will be performed using a daily subcutaneous dosing schedule X 5 days with total prodrug dose equal to the IC 10 defined in the single I.P. dosing studies.
- prodrug dosing will commence when the means xenograft volumes in each group reach 5OMM 3 .
- Mean tumor volumes of prodrug-treated relative to control-treated animals will be plotted using descriptive statistics with graphical analysis.
- Statistical tests (single factor ANOVA) will be applied to compare xenograft volume differences between treatment groups. Log-transformation will be applied for
- Groups of 6 CD-I nu/nu athymic mice (Charles River Laboratories) (3 male, 3 female) are injected with a single dose of prodrug via the intraperitoneal (I.P.) route. Dosing will begin empirically with the initial dose defined by the amount of a prodrug to achieve a serum concentration equal to the IC 50 in vitro, assuming the volume of distribution of the prodrug to be restricted to the total body water (TBW) compartment of the mouse (0.6 X body mass in grams TBW mouse ). If no toxicity is observed at this level, then dose escalation proceeds in groups of 6 mice at half-log intervals. If toxicity is encountered at the empiric dose level, then repeat dose escalation experiments beginning at
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JP2000528661A JP2002500880A (en) | 1998-01-23 | 1999-01-22 | Therapeutic agents catalyzed by enzymes |
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HK1039520A1 (en) | 2002-04-26 |
IL137164A0 (en) | 2001-07-24 |
US7601703B2 (en) | 2009-10-13 |
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US6339151B1 (en) | 2002-01-15 |
CA2317505A1 (en) | 1999-07-29 |
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EP1045897A4 (en) | 2000-10-25 |
EP1045897A1 (en) | 2000-10-25 |
EP1167972A2 (en) | 2002-01-02 |
EP1167972B1 (en) | 2017-03-08 |
EP1045897B1 (en) | 2002-01-30 |
DE69900841T2 (en) | 2002-10-02 |
DK1045897T3 (en) | 2002-05-13 |
PT1045897E (en) | 2002-07-31 |
EP1167972A3 (en) | 2006-05-24 |
CA2317505C (en) | 2011-01-04 |
BR9907736A (en) | 2000-10-17 |
DE69900841D1 (en) | 2002-03-14 |
JP2001220397A (en) | 2001-08-14 |
ATE212661T1 (en) | 2002-02-15 |
JP3265304B2 (en) | 2002-03-11 |
HK1030624A1 (en) | 2001-05-11 |
ES2172303T3 (en) | 2002-09-16 |
CN1192102C (en) | 2005-03-09 |
CN1291228A (en) | 2001-04-11 |
JP2002500880A (en) | 2002-01-15 |
TWI244924B (en) | 2005-12-11 |
US6245750B1 (en) | 2001-06-12 |
AU753155B2 (en) | 2002-10-10 |
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