WO1999015895A1 - Method of arraying nanoparticles and macromolecules on surfaces - Google Patents
Method of arraying nanoparticles and macromolecules on surfaces Download PDFInfo
- Publication number
- WO1999015895A1 WO1999015895A1 PCT/SE1998/001712 SE9801712W WO9915895A1 WO 1999015895 A1 WO1999015895 A1 WO 1999015895A1 SE 9801712 W SE9801712 W SE 9801712W WO 9915895 A1 WO9915895 A1 WO 9915895A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- created
- surface defects
- macromolecules
- nanoparticles
- defects
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82B—NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF
- B82B3/00—Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
- B01J2219/00648—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
Definitions
- the invention relates to a method of arraying nanoparticles and macromolecules on surfaces in order to obtain an arrayed immobilisation of said particles in a desired pattern.
- the object of the present invention is to provide a method of arraying nanoparticles and macromolecules on surfaces.
- a pattern of surface defects are created on a surface, the form, appearance and mapping out of the surface defects being adapted to those nanoparticles and/or macromolecules which are to be arrayed.
- holes and/or rises having a diameter and a depth and a height, respectively, within the interval of 1 - 50 nanometers, and a mutual distance within the interval of 0.1 - 1000 nanometers are created.
- surface defects in the form of lines are created in the surface.
- a surface comprising organic or inorganic material.
- the surface is coated with polymers of inorganic or organic material before the creation of surface defects, and subsequently selective surface modifications are made in order to provide the area containing the surface defects with desired chemical or mechanical characteristics.
- the thickness of the coating is varied throughout the surface whereby holes with different depths and/or diameters can be created.
- the surface defects are created by using finely focused ion beam technique
- the source of ions is indium, gallium, platinum, gold, silver or copper.
- the surface defects are created by nanoindenting with a diamond- pointed probe used in scanning probe microscopy. Short description of the drawings
- Fig. 1A and B show arrays of surface defects created by a finely focused ion beam before and after exposure to a solution of human serum albumin
- Fig. 2A and B show arrays of surface defects created by nanoindenting .
- a variation of the diameter of the holes makes it possible to vary the number of molecules or paticles being adhered to these.
- the holes or rises are made with molecular dimensions, which means that single molecules or particles can be adhered to the underlayer.
- the ion source can be variated so that a material which is most suitable for the intended application can be deposited on the defect made in the surface, to which material a stronger immobilisation of a macromolecule or particle can be made.
- Protein molecules are strongly adsorbed to platinum and palladium, and in this case the ion source for the finely focused ion beam should be platinum or palladium.
- Another method would be sputtering of gold onto the surface defects, to which tiolated proteins or particles are immobilized.
- Defects are created in the surface with the aid of f inely focused ion beam technique or nanoindenting technique .
- E . g . lines with a suitable depth and diameter are made in the surface.
- Nanoparticle of organic or inorganic origin are added to the surface and they will collect in the created defects.
- Excess nanoparticles are removed mechanically with pressurized air, shaking, centrifugation or any other suitable method for removal . Thereafter the surface provided with particles is heated to melting point in order to obtain a continuous thread of the material, intended for different uses such as information transfer.
- nanoparticles or macromolecules fitting in the holes can be added.
- the nanoparticles may e.g. consist of the well known bioactive substance hydroxyapatit or other types of bioactive materials to which the cellular surface adheres.
- suitable macromolecules are the so called integrin family, i.e. vitronektin and fibronektin, which are so called cellular "glues" for adhering of cells to different surfaces.
- Biomolecular memories Since the surface can be modulated in x- , y- , and z- directions there arises the possibility to create a surface above this topographical chart, which surface varies in x- , y- and z-directions physically-chemically by adding e.g. peptides or amino acids to positions on the surface having been ion beam treated or nanoindented.
- voltage differentials can arise, which can be used as signal generators.
- Other possibilities are the use of the artificially created memory surface for separation or analysis.
- the memory surface can be used for the development of drugs, where a certain membrane structure corresponds with a pharmaceutically active molecule structure .
- Analytical measuring methods and separation methods for analysis and separation of single molecules and particles can be coated with polymers of inorganic or organic material. With the ion beam can then selective surface modifications be done, which result in that the ion beam treated area will have other chemical or mechanical characteristics. To these areas a selective particle or molecule binding-in can be obtained. Since the applied surface coating can be made with varying thickness there is also the possibility of making holes with different depths but also different diameters, e.g. for molecular filter applications .
- Biocatalytical systems most often exists bound to sur- faces.
- the catalyse is achieved among other things because of reduced diffusion distances and because high local concentrations can arise.
- Other examples of important factors for increased biocatalyse exist in photosynthetic systems where voltage differences are used for electron cascades.
- a positioning of molecules means that these characteristics can be used to a full extent, which can be performed with finely focused ion beam technique, but also with nanoindenting.
- a well ordered array of defects was prepared on a silicon surface, using a finely focused ion beam with 30 keV indium ions m a 11 pA beam current.
- the beam spot size was 15 nm, and approximately a 10 second milling time was used for each 5 ⁇ m x 5 m area.
- the total array consisted of 16 milling areas, each with an array of holes with an estimated diameter of 50 nm.
- the spacing between individual defects was about 160 nm.
- the defect array was imaged with a scanning force micro- scope run m tapping mode (TM-3FM) under ambient conditions.
- the scanning force microscope employed was a Nanoscope III 115 (Digital Instruments Inc., Santa Barbara, CA, USA).
- Human serum albumin (HSA) (Sigma Chemical Co., St Louis,
- HEPES buffer N- [2-Hydroxy- ethyl] piperazme-N' - [2-ethanesulfon ⁇ c acid]
- pH 7.5 pH 7.5
- 30 ⁇ l of the HSA solution was placed on the silicon so that it covered the array, and was then rinsed off with 1 ml of HEPES buffer after 2 minutes. The surface was then dried using a flow of nitrogen, and probed again with TM-SFM.
- the holes have only very slightly elevated rims (Figure, 1A) .
- the depth of the holes may not de detected with the AFM, due to the bulkiness of the tip compared to the size of the hole .
- the rims of the holes were decorated with several molecules of HSA (Figure, IB) . There were very few or none of the HSA molecules adsorbed on the areas between the defects ordered in arrays. There was clearly a selective adsorption of HSA molecules to the well ordered array of defects.
- the image size in Figs 1A and IB, respectively is l ⁇ m x l ⁇ m.
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2000513142A JP2001517561A (en) | 1997-09-24 | 1998-09-23 | Method for arranging nanoparticles and polymers on a surface |
CA002304502A CA2304502A1 (en) | 1997-09-24 | 1998-09-23 | Method of arraying nanoparticles and macromolecules on surfaces |
EP98945729A EP1029242A1 (en) | 1997-09-24 | 1998-09-23 | Method of arraying nanoparticles and macromolecules on surfaces |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9703447-4 | 1997-09-24 | ||
SE9703447A SE9703447D0 (en) | 1997-09-24 | 1997-09-24 | Method of Arranging Nanoparticles and Macromolecules on Surface |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1999015895A1 true WO1999015895A1 (en) | 1999-04-01 |
Family
ID=20408356
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/SE1998/001712 WO1999015895A1 (en) | 1997-09-24 | 1998-09-23 | Method of arraying nanoparticles and macromolecules on surfaces |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1029242A1 (en) |
JP (1) | JP2001517561A (en) |
CA (1) | CA2304502A1 (en) |
SE (1) | SE9703447D0 (en) |
WO (1) | WO1999015895A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001060316A2 (en) * | 2000-02-18 | 2001-08-23 | Sven Oscarsson | Method for the positioning of macromolecules and particles |
WO2003023402A1 (en) * | 2001-09-12 | 2003-03-20 | Sven Oscarsson | Immobilisation method and surfaces produced using said method |
WO2003087291A2 (en) * | 2002-04-11 | 2003-10-23 | Spire Corporation | Bioanalytical array having an ion beam treated surface |
CN100484867C (en) * | 2004-10-22 | 2009-05-06 | 中国科学院上海应用物理研究所 | Method for separating and replacing nanometer particles |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5252294A (en) * | 1988-06-01 | 1993-10-12 | Messerschmitt-Bolkow-Blohm Gmbh | Micromechanical structure |
US5512131A (en) * | 1993-10-04 | 1996-04-30 | President And Fellows Of Harvard College | Formation of microstamped patterns on surfaces and derivative articles |
US5587273A (en) * | 1993-01-21 | 1996-12-24 | Advanced Microbotics Corporation | Molecularly imprinted materials, method for their preparation and devices employing such materials |
GB2308369A (en) * | 1995-12-20 | 1997-06-25 | Marconi Gec Ltd | Imprinted materials; separation/sensing of small molecules |
US5776748A (en) * | 1993-10-04 | 1998-07-07 | President And Fellows Of Harvard College | Method of formation of microstamped patterns on plates for adhesion of cells and other biological materials, devices and uses therefor |
-
1997
- 1997-09-24 SE SE9703447A patent/SE9703447D0/en unknown
-
1998
- 1998-09-23 EP EP98945729A patent/EP1029242A1/en not_active Withdrawn
- 1998-09-23 JP JP2000513142A patent/JP2001517561A/en active Pending
- 1998-09-23 CA CA002304502A patent/CA2304502A1/en not_active Abandoned
- 1998-09-23 WO PCT/SE1998/001712 patent/WO1999015895A1/en not_active Application Discontinuation
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5252294A (en) * | 1988-06-01 | 1993-10-12 | Messerschmitt-Bolkow-Blohm Gmbh | Micromechanical structure |
US5587273A (en) * | 1993-01-21 | 1996-12-24 | Advanced Microbotics Corporation | Molecularly imprinted materials, method for their preparation and devices employing such materials |
US5512131A (en) * | 1993-10-04 | 1996-04-30 | President And Fellows Of Harvard College | Formation of microstamped patterns on surfaces and derivative articles |
US5776748A (en) * | 1993-10-04 | 1998-07-07 | President And Fellows Of Harvard College | Method of formation of microstamped patterns on plates for adhesion of cells and other biological materials, devices and uses therefor |
GB2308369A (en) * | 1995-12-20 | 1997-06-25 | Marconi Gec Ltd | Imprinted materials; separation/sensing of small molecules |
Non-Patent Citations (4)
Title |
---|
ACC. CHEM. RES., Volume 28, 1995, AMIT KUMAR et al., "Patterned Self-Assembled Monolayers and Meso-Scale Phenomena", pages 219-226. * |
DIALOG INFORMATION SERVICES, File 434, Scisearch, Dialog Accession No. 14680237, KIM E., "2-Dimensional and 3-Dimensional Crystallization of Polymeric Microspheres by Micromolding in Capillaries"; & ADVANCED MATERIALS, Mar. 1996, Vol. 8, No. 3, p. 245. * |
DIALOG INFORMATION SERVICES, File 434, Scisearch, Dialog Accession No. 15305073, PANTANO P., "Ordered Nanowell Arrays"; & CHEMISTRY OF MATERIALS, Dec. 1996, Vol. 8, No. 12, pp. 2832-2835. * |
TIBTECH, Volume 13, June 1995, MILAN MRKSICH et al., "Patterning Self-Assembled Monolayers Using Microcontact Printing: A New Technology for Biosensors", pages 231-232. * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001060316A2 (en) * | 2000-02-18 | 2001-08-23 | Sven Oscarsson | Method for the positioning of macromolecules and particles |
WO2001060316A3 (en) * | 2000-02-18 | 2002-08-15 | Sven Oscarsson | Method for the positioning of macromolecules and particles |
WO2003023402A1 (en) * | 2001-09-12 | 2003-03-20 | Sven Oscarsson | Immobilisation method and surfaces produced using said method |
WO2003087291A2 (en) * | 2002-04-11 | 2003-10-23 | Spire Corporation | Bioanalytical array having an ion beam treated surface |
WO2003087291A3 (en) * | 2002-04-11 | 2003-12-24 | Spire Corp | Bioanalytical array having an ion beam treated surface |
US7687256B2 (en) | 2002-04-11 | 2010-03-30 | Spire Corporation | Surface activated biochip |
CN100484867C (en) * | 2004-10-22 | 2009-05-06 | 中国科学院上海应用物理研究所 | Method for separating and replacing nanometer particles |
Also Published As
Publication number | Publication date |
---|---|
EP1029242A1 (en) | 2000-08-23 |
CA2304502A1 (en) | 1999-04-01 |
JP2001517561A (en) | 2001-10-09 |
SE9703447D0 (en) | 1997-09-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20020114987A1 (en) | Method of arraying nanoparticles and macromolecules on surfaces | |
Wadu‐Mesthrige et al. | Immobilization of proteins on self‐assembled monolayers | |
Wadu-Mesthrige et al. | Fabrication of nanometer-sized protein patterns using atomic force microscopy and selective immobilization | |
US7842344B2 (en) | Peptide and protein arrays and direct-write lithographic printing of peptides and proteins | |
Wagner et al. | Covalent immobilization of native biomolecules onto Au (111) via N-hydroxysuccinimide ester functionalized self-assembled monolayers for scanning probe microscopy | |
CA2462833C (en) | Protein and peptide nanoarrays | |
US6228326B1 (en) | Arrays of independently-addressable supported fluid bilayer membranes | |
Christman et al. | Nanopatterning proteins and peptides | |
Butt et al. | Imaging the membrane protein bacteriorhodopsin with the atomic force microscope | |
Patel et al. | A scanning probe microscopy study of the physisorption and chemisorption of protein molecules onto carboxylate terminated self-assembled monolayers. | |
Davies et al. | Use of scanning probe microscopy and surface plasmon resonance as analytical tools in the study of antibody-coated microtiter wells | |
JP2005524829A (en) | Improved structured functional binding matrix for biomolecules | |
AU2001280999A1 (en) | Biosensor arrays and methods | |
US20080242559A1 (en) | Protein and peptide arrays | |
WO2004067191A2 (en) | Alteration of surface affinities | |
Kossek et al. | Localization of individual biomolecules on sensor surfaces | |
EP1029242A1 (en) | Method of arraying nanoparticles and macromolecules on surfaces | |
JPH095338A (en) | Method for forming chemically discriminated picture by scanning atomic power microscope | |
Zhou et al. | Reversible hydrophobic barriers introduced by microcontact printing: application to protein microarrays | |
Cooper et al. | The imaging of streptavidin and avidin using scanning tunnelling microscopy | |
Wilde et al. | Molecular patterning on carbon based surfaces through photobiotin activationElectronic Supplementary Information available. See http://www. rsc. org/suppdata/an/b0/b008475l | |
US20030134273A1 (en) | Combined molecular binding detection through force microscopy and mass spectrometry | |
Liu et al. | Immobilization of a Monolayer of Bovine Serum Albumin on Gold Nanoparticles for Stereo‐specified Recognition of Dansyl‐norvaline | |
Lahiri et al. | Lipid microarrays | |
You et al. | A scanning tunnelling microscopic study of site-specifically immobilized immunoglobulin G on gold |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): CA JP US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
ENP | Entry into the national phase |
Ref document number: 2304502 Country of ref document: CA Ref country code: CA Ref document number: 2304502 Kind code of ref document: A Format of ref document f/p: F |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1998945729 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 1998945729 Country of ref document: EP |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 1998945729 Country of ref document: EP |