WO1998027230A1 - Methods and compositions for polypeptide engineering - Google Patents
Methods and compositions for polypeptide engineering Download PDFInfo
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- WO1998027230A1 WO1998027230A1 PCT/US1997/024239 US9724239W WO9827230A1 WO 1998027230 A1 WO1998027230 A1 WO 1998027230A1 US 9724239 W US9724239 W US 9724239W WO 9827230 A1 WO9827230 A1 WO 9827230A1
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- WIPO (PCT)
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- protein
- dna
- dna substrate
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- library
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/45—Transferases (2)
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- A—HUMAN NECESSITIES
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
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- C07K14/52—Cytokines; Lymphokines; Interferons
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
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- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
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- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
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- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/14011—Details ssDNA Bacteriophages
- C12N2795/14041—Use of virus, viral particle or viral elements as a vector
- C12N2795/14043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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Abstract
Description
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Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU57292/98A AU732146B2 (en) | 1996-12-18 | 1997-12-17 | Methods and compositions for polypeptide engineering |
JP52805498A JP5008784B2 (en) | 1996-12-18 | 1997-12-17 | Methods and compositions for polypeptide manipulation |
IL13019097A IL130190A0 (en) | 1996-12-18 | 1997-12-17 | Methods and compositions for polypeptide engineering |
CA2274319A CA2274319C (en) | 1996-12-18 | 1997-12-17 | Methods and compositions for polypeptide engineering |
EP97953571A EP0946755A4 (en) | 1996-12-18 | 1997-12-17 | Methods and compositions for polypeptide engineering |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/769,062 US6335160B1 (en) | 1995-02-17 | 1996-12-18 | Methods and compositions for polypeptide engineering |
US08/769,062 | 1996-12-18 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1998027230A1 true WO1998027230A1 (en) | 1998-06-25 |
Family
ID=25084330
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1997/024239 WO1998027230A1 (en) | 1996-12-18 | 1997-12-17 | Methods and compositions for polypeptide engineering |
Country Status (12)
Country | Link |
---|---|
US (20) | US6335160B1 (en) |
EP (5) | EP1149905B1 (en) |
JP (2) | JP5008784B2 (en) |
KR (1) | KR20000069591A (en) |
AT (1) | ATE481480T1 (en) |
AU (1) | AU732146B2 (en) |
CA (2) | CA2274319C (en) |
DE (1) | DE69739996D1 (en) |
DK (2) | DK2202308T3 (en) |
ES (1) | ES2351673T3 (en) |
IL (1) | IL130190A0 (en) |
WO (1) | WO1998027230A1 (en) |
Cited By (268)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0920496A1 (en) * | 1997-03-25 | 1999-06-09 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
FR2782323A1 (en) * | 1998-08-12 | 2000-02-18 | Proteus | In vitro recombination of polynucleotide fragments to obtain sequences with improved properties involves ligation on an assembly matrix |
WO2000009727A2 (en) * | 1998-08-12 | 2000-02-24 | Maxygen, Inc. | Dna shuffling to produce herbicide selective crops |
WO2000020573A2 (en) * | 1998-10-07 | 2000-04-13 | Maxygen, Inc. | Dna shuffling to produce nucleic acids for mycotoxin detoxification |
WO2000042561A2 (en) * | 1999-01-19 | 2000-07-20 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
WO2000053744A2 (en) * | 1999-03-09 | 2000-09-14 | Diversa Corporation | End selection in directed evolution |
WO2000061740A1 (en) * | 1999-04-10 | 2000-10-19 | Maxygen, Inc. | Modified lipid production |
US6159687A (en) * | 1997-03-18 | 2000-12-12 | Novo Nordisk A/S | Methods for generating recombined polynucleotides |
WO2001004287A1 (en) * | 1999-07-07 | 2001-01-18 | Maxygen Aps | A method for preparing modified polypeptides |
EP1073710A2 (en) * | 1999-02-04 | 2001-02-07 | Diversa Corporation | Non-stochastic generation of genetic vaccines and enzymes |
WO2001012791A1 (en) * | 1999-08-12 | 2001-02-22 | Maxygen, Inc. | Dna shuffling of dioxygenase genes for production of industrial chemicals |
WO2001029211A2 (en) * | 1999-10-19 | 2001-04-26 | Enchira Biotechnology Corporation | Method for directed evolution by random chimeragenesis on transient templates |
WO2001029212A1 (en) * | 1999-10-19 | 2001-04-26 | Enchira Biotechnology Corporation | Methods for chimeragenesis of whole genomes or large polynucleotides |
WO2001032712A2 (en) * | 1999-11-03 | 2001-05-10 | Maxygen, Inc. | Antibody diversity generation |
US6238884B1 (en) | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
US6242211B1 (en) | 1996-04-24 | 2001-06-05 | Terragen Discovery, Inc. | Methods for generating and screening novel metabolic pathways |
WO2001064864A2 (en) * | 2000-02-28 | 2001-09-07 | Maxygen, Inc. | Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation |
US6287862B1 (en) | 1997-01-17 | 2001-09-11 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
US6303848B1 (en) | 1998-01-16 | 2001-10-16 | Large Scale Biology Corporation | Method for conferring herbicide, pest, or disease resistance in plant hosts |
US6319714B1 (en) | 1999-01-19 | 2001-11-20 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
WO2001088173A2 (en) * | 2000-05-16 | 2001-11-22 | Hercules Incorporated | Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics |
US6337186B1 (en) | 1998-06-17 | 2002-01-08 | Maxygen, Inc. | Method for producing polynucleotides with desired properties |
WO2002004630A2 (en) * | 2000-07-10 | 2002-01-17 | Ambion, Inc. | Methods for recombinatorial nucleic acid synthesis |
WO2002006469A2 (en) * | 2000-07-18 | 2002-01-24 | Enchira Biotechnology Corporation | Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids |
US6352859B1 (en) | 1997-01-17 | 2002-03-05 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
US6358709B1 (en) | 1995-12-07 | 2002-03-19 | Diversa Corporation | End selection in directed evolution |
US6358712B1 (en) | 1999-01-05 | 2002-03-19 | Trustee Of Boston University | Ordered gene assembly |
EP1192280A1 (en) * | 1999-06-14 | 2002-04-03 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
US6391640B1 (en) | 1994-02-17 | 2002-05-21 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US6399383B1 (en) | 1997-10-28 | 2002-06-04 | Maxygen, Inc. | Human papilloma virus vectors |
US6420175B1 (en) | 1994-02-17 | 2002-07-16 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6426185B1 (en) | 1998-01-16 | 2002-07-30 | Large Scale Biology Corporation | Method of compiling a functional gene profile in a plant by transfecting a nucleic acid sequence of a donor plant into a different host plant in an anti-sense orientation |
US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
US6468745B1 (en) | 1998-01-16 | 2002-10-22 | Large Scale Biology Corporation | Method for expressing a library of nucleic acid sequence variants and selecting desired traits |
WO2002090536A2 (en) * | 2001-05-10 | 2002-11-14 | Novozymes A/S | A method for producing recombined polynucleotides |
US6483011B1 (en) | 1998-11-10 | 2002-11-19 | Maxygen, Inc. | Modified ADP-glucose pyrophosphorylase for improvement and optimization of plant phenotypes |
WO2002099080A2 (en) * | 2001-06-05 | 2002-12-12 | Gorilla Genomics, Inc. | Methods for low background cloning of dna using long oligonucleotides |
US6500617B1 (en) | 1998-05-01 | 2002-12-31 | Maxygen, Inc. | Optimization of pest resistance genes using DNA shuffling |
WO2003010311A2 (en) * | 2001-07-23 | 2003-02-06 | Dsm Ip Assets B.V. | Process for preparing variant polynucleotides |
US6531316B1 (en) | 1999-03-05 | 2003-03-11 | Maxyag, Inc. | Encryption of traits using split gene sequences and engineered genetic elements |
US6596539B1 (en) | 1997-10-31 | 2003-07-22 | Maxygen, Inc. | Modification of virus tropism and host range by viral genome shuffling |
US6605430B1 (en) | 1998-08-12 | 2003-08-12 | Maxygen, Inc. | DNA shuffling of monooxygenase genes for production of industrial chemicals |
WO2003075129A2 (en) | 2002-03-01 | 2003-09-12 | Maxygen, Inc. | Methods, systems, and software for identifying functional bio-molecules |
WO2003078583A2 (en) | 2002-03-09 | 2003-09-25 | Maxygen, Inc. | Optimization of crossover points for directed evolution |
US6632673B1 (en) | 1999-08-12 | 2003-10-14 | Pfizer, Inc. | Directing the ratio of B2:B1 avermectins in Streptomyces avermitilis host cells |
EP1352959A1 (en) * | 1997-01-24 | 2003-10-15 | Bioinvent International AB | A method for in vitro molecular evolution of protein function |
US6686515B1 (en) | 1999-11-23 | 2004-02-03 | Maxygen, Inc. | Homologous recombination in plants |
US6713279B1 (en) | 1995-12-07 | 2004-03-30 | Diversa Corporation | Non-stochastic generation of genetic vaccines and enzymes |
US6713281B2 (en) | 1995-12-07 | 2004-03-30 | Diversa Corporation | Directed evolution of thermophilic enzymes |
US6740506B2 (en) | 1995-12-07 | 2004-05-25 | Diversa Corporation | End selection in directed evolution |
EP1421203A2 (en) * | 2001-05-17 | 2004-05-26 | Diversa Corporation | Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof |
US6759226B1 (en) | 2000-05-24 | 2004-07-06 | Third Wave Technologies, Inc. | Enzymes for the detection of specific nucleic acid sequences |
US6764835B2 (en) | 1995-12-07 | 2004-07-20 | Diversa Corporation | Saturation mutageneis in directed evolution |
WO2004090099A2 (en) | 2003-04-04 | 2004-10-21 | Diversa Corporation | Pectate lyases, nucleic acids encoding them and methods for making and using them |
US6858422B2 (en) | 2000-07-13 | 2005-02-22 | Codexis, Inc. | Lipase genes |
WO2005021714A2 (en) | 2003-08-11 | 2005-03-10 | Diversa Corporation | Laccases, nucleic acids encoding them and methods for making and using them |
US6902918B1 (en) | 1998-05-21 | 2005-06-07 | California Institute Of Technology | Oxygenase enzymes and screening method |
US6917882B2 (en) | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
EP1558748A2 (en) * | 2002-11-01 | 2005-08-03 | Promega Corporation | Cell lysis compositions, methods of use, apparatus, and kit |
US6939689B2 (en) | 1995-12-07 | 2005-09-06 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
WO2005082077A2 (en) | 2004-02-25 | 2005-09-09 | Pioneer Hi-Bred International, Inc. | Novel bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof |
US6951719B1 (en) | 1999-08-11 | 2005-10-04 | Proteus S.A. | Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained |
US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
AU2002254773B2 (en) * | 2001-05-03 | 2005-12-08 | Rensselaer Polytechnic Institute | Novel methods of directed evolution |
WO2005123929A2 (en) | 2004-06-09 | 2005-12-29 | Pioneer Hi-Bred International, Inc. | Plastid transit peptides |
WO2006009676A2 (en) | 2004-06-16 | 2006-01-26 | Diversa Corporation | Compositions and methods for enzymatic decolorization of chlorophyll |
US6991922B2 (en) | 1998-08-12 | 2006-01-31 | Proteus S.A. | Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation |
US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
US7045289B2 (en) | 1991-09-09 | 2006-05-16 | Third Wave Technologies, Inc. | Detection of RNA Sequences |
US7074590B2 (en) | 2000-06-23 | 2006-07-11 | Maxygen, Inc. | Chimeric promoters |
US7087415B2 (en) | 2000-07-31 | 2006-08-08 | Athena Biotechnologies, Inc. | Methods and compositions for directed gene assembly |
US7094875B2 (en) | 2000-06-23 | 2006-08-22 | Maxygen, Inc. | Co-stimulatory polypeptides |
US7105297B2 (en) | 1994-02-17 | 2006-09-12 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
WO2006101584A2 (en) | 2005-03-15 | 2006-09-28 | Diversa Corporation | Cellulases, nucleic acids encoding them and methods for making and using them |
US7115712B1 (en) | 1999-12-02 | 2006-10-03 | Maxygen, Inc. | Cytokine polypeptides |
US7150982B2 (en) | 1991-09-09 | 2006-12-19 | Third Wave Technologies, Inc. | RNA detection assays |
US20070037254A1 (en) * | 1999-07-12 | 2007-02-15 | Genentech, Inc. | Expression Vectors and Methods |
US7226768B2 (en) | 2001-07-20 | 2007-06-05 | The California Institute Of Technology | Cytochrome P450 oxygenases |
WO2007095398A2 (en) | 2006-02-14 | 2007-08-23 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
WO2008036863A2 (en) | 2006-09-21 | 2008-03-27 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
US7354734B2 (en) | 2003-08-25 | 2008-04-08 | Funzyme Biotechnologies Sa | Fungal proteins and nucleic acids encoding same |
US7384387B1 (en) | 1999-02-11 | 2008-06-10 | Maxygen, Inc. | High throughput mass spectrometry |
WO2008080093A2 (en) | 2006-12-21 | 2008-07-03 | Verenium Corporation | Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them |
DE102007003143A1 (en) | 2007-01-16 | 2008-07-17 | Henkel Kgaa | New alkaline protease from Bacillus gibsonii and detergents and cleaners containing this novel alkaline protease |
WO2008095033A2 (en) | 2007-01-30 | 2008-08-07 | Verenium Corporation | Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them |
WO2008098198A2 (en) | 2007-02-08 | 2008-08-14 | The California Institute Of Technology | Alkane oxidation by modified hydroxylases |
WO2008103248A1 (en) | 2007-02-08 | 2008-08-28 | Codexis, Inc. | Ketoreductases and uses thereof |
WO2008105797A2 (en) | 2006-06-30 | 2008-09-04 | Bristol-Myers Squibb Company | Polynucleotides encoding novel pcsk9 variants |
US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
US7435570B2 (en) | 2003-08-11 | 2008-10-14 | California Institute Of Technology | Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use |
US7465567B2 (en) | 2001-04-16 | 2008-12-16 | California Institute Of Technology | Peroxide-driven cytochrome P450 oxygenase variants |
WO2009029554A2 (en) | 2007-08-24 | 2009-03-05 | Codexis, Inc. | Improved ketoreductase polypeptides for the stereoselective production of (r)-3-hydroxythiolane |
WO2009045627A2 (en) | 2007-10-03 | 2009-04-09 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
US7524664B2 (en) | 2003-06-17 | 2009-04-28 | California Institute Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome P450 |
DE102007051092A1 (en) | 2007-10-24 | 2009-04-30 | Henkel Ag & Co. Kgaa | Subtilisin from Becillus pumilus and detergents and cleaners containing this new subtilisin |
WO2009058564A2 (en) | 2007-11-01 | 2009-05-07 | Maxygen, Inc. | Immunosuppressive polypeptides and nucleic acids |
WO2009088949A1 (en) | 2008-01-03 | 2009-07-16 | Verenium Corporation | Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them |
EP2112227A1 (en) | 2006-03-07 | 2009-10-28 | Cargill, Incorporated | Aldolases, nucleic acids encoding them and methods for making and using them |
WO2009152336A1 (en) | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
WO2010025238A2 (en) | 2008-08-27 | 2010-03-04 | Codexis, Inc. | Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine |
WO2010025395A2 (en) | 2008-08-29 | 2010-03-04 | Verenium Corporation | Hydrolases, nucleic acids encoding them and methods for making and using them |
EP2194133A2 (en) | 2003-03-06 | 2010-06-09 | Verenium Corporation | Amylases, nucleic acids encoding them and methods for making and using them |
WO2010081053A2 (en) | 2009-01-08 | 2010-07-15 | Codexis, Inc. | Transaminase polypeptides |
EP2216403A2 (en) | 2006-02-02 | 2010-08-11 | Verenium Corporation | Esterases and related nucleic acids and methods |
US7795030B2 (en) | 1994-02-17 | 2010-09-14 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
WO2010107644A2 (en) | 2009-03-17 | 2010-09-23 | Codexis, Inc. | Variant endoglucanases and related polynucleotides |
WO2010120557A1 (en) | 2009-03-31 | 2010-10-21 | Codexis, Inc. | Improved endoglucanases |
WO2010135588A2 (en) | 2009-05-21 | 2010-11-25 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
WO2010144103A1 (en) | 2009-06-11 | 2010-12-16 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
WO2010148128A1 (en) | 2009-06-16 | 2010-12-23 | Codexis, Inc. | Beta-glucosidase variant enzymes and related polynucleotides |
EP2270234A1 (en) | 1997-12-08 | 2011-01-05 | California Institute of Technology | Method for creating polynucleotide and polypeptide sequences |
WO2011005527A2 (en) | 2009-06-22 | 2011-01-13 | Codexis, Inc. | Ketoreductase-mediated stereoselective route to alpha chloroalcohols |
EP2275536A1 (en) | 2002-08-06 | 2011-01-19 | Verdia, Inc. | AP1 amine oxidase variants |
WO2011022548A2 (en) | 2009-08-19 | 2011-02-24 | Codexis, Inc. | Ketoreductase polypeptides for the preparation of phenylephrine |
WO2011022597A1 (en) | 2009-08-20 | 2011-02-24 | Pioneer Hi-Bred International, Inc. | Functional expression of shuffled yeast nitrate transporter (ynti) in maize to improve nitrate uptake under low nitrate environment |
WO2011028708A1 (en) | 2009-09-04 | 2011-03-10 | Codexis, Inc. | Variant cbh2 cellulases and related polynucleotides |
EP2298871A1 (en) | 2002-04-19 | 2011-03-23 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
WO2011046815A1 (en) | 2009-10-16 | 2011-04-21 | Bunge Oils, Inc. | Oil degumming methods |
WO2011046812A1 (en) | 2009-10-16 | 2011-04-21 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
EP2322629A2 (en) | 2003-04-29 | 2011-05-18 | Pioneer Hi-Bred International Inc. | Novel glyphosate-n-acetyltransferase (GAT) genes |
WO2011066187A1 (en) | 2009-11-25 | 2011-06-03 | Codexis, Inc. | Recombinant thermoascus aurantiacus beta-glucosidase variants for production of fermentable sugars from cellulosic biomass |
WO2011071982A2 (en) | 2009-12-08 | 2011-06-16 | Codexis, Inc. | Synthesis of prazole compounds |
WO2011082304A1 (en) | 2009-12-31 | 2011-07-07 | Pioneer Hi-Bred International, Inc. | Engineering plant resistance to diseases caused by pathogens |
US8026085B2 (en) | 2006-08-04 | 2011-09-27 | California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
EP2385108A1 (en) | 2006-03-07 | 2011-11-09 | Verenium Corporation | Aldolases, nucleic acids encoding them and methods for making and using them |
WO2011140219A1 (en) | 2010-05-04 | 2011-11-10 | Codexis, Inc. | Biocatalysts for ezetimibe synthesis |
WO2011143274A1 (en) | 2010-05-10 | 2011-11-17 | Perseid Therapeutics | Polypeptide inhibitors of vla4 |
EP2397486A1 (en) | 2006-09-21 | 2011-12-21 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
WO2011159910A2 (en) | 2010-06-17 | 2011-12-22 | Codexis, Inc. | Biocatalysts and methods for the synthesis of (s)-3-(1-aminoethyl)-phenol |
WO2012003299A2 (en) | 2010-06-30 | 2012-01-05 | Codexis, Inc. | Highly stable beta-class carbonic anhydrases useful in carbon capture systems |
EP2404931A1 (en) | 2003-07-02 | 2012-01-11 | Verenium Corporation | Glucanases, nucleic acids encoding them and methods for making and using them |
WO2012006114A2 (en) | 2010-06-28 | 2012-01-12 | Codexis, Inc. | Fatty alcohol forming acyl reductases (fars) and methods of use thereof |
EP2415864A1 (en) | 2006-02-10 | 2012-02-08 | Verenium Corporation | Oligomerase-2 (or beta-xylosidase) enzymes, nucleic acids encoding them and methods for making and using them |
WO2012021785A1 (en) | 2010-08-13 | 2012-02-16 | Pioneer Hi-Bred International, Inc. | Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (hppd) activity |
WO2012024698A1 (en) | 2010-08-20 | 2012-02-23 | Codexis, Inc. | Use of glycoside hydrolase 61 family proteins in processing of cellulose |
EP2444413A1 (en) | 2006-08-04 | 2012-04-25 | Verenium Corporation | Methods for oil or gas well drilling, washing and/or fracturing |
US8178333B2 (en) | 2008-06-24 | 2012-05-15 | Codexis, Inc. | Biocatalytic processes for the preparation of substantially stereomerically pure fused bicyclic proline compounds |
WO2012078800A2 (en) | 2010-12-08 | 2012-06-14 | Codexis, Inc. | Biocatalysts and methods for the synthesis of armodafinil |
US8252559B2 (en) | 2006-08-04 | 2012-08-28 | The California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
US8273554B2 (en) | 2008-08-29 | 2012-09-25 | Codexis, Inc. | Ketoreductase polypeptides for the stereoselective production of (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one |
US8288141B2 (en) | 2008-08-27 | 2012-10-16 | Codexis, Inc. | Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine |
US8288131B2 (en) | 2008-08-27 | 2012-10-16 | Codexis, Inc. | Ketoreductase polypeptides and uses thereof |
WO2012142302A2 (en) | 2011-04-13 | 2012-10-18 | Codexis, Inc. | Biocatalytic process for preparing eslicarbazepine and analogs thereof |
EP2559704A1 (en) | 2007-02-08 | 2013-02-20 | Domantis Limited | Antibody single variable domains against serum albumin |
US8383346B2 (en) | 2008-06-13 | 2013-02-26 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
WO2013028701A1 (en) | 2011-08-22 | 2013-02-28 | Codexis, Inc. | Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity |
WO2013036861A1 (en) | 2011-09-08 | 2013-03-14 | Codexis, Inc | Biocatalysts and methods for the synthesis of substituted lactams |
WO2013074650A1 (en) | 2011-11-18 | 2013-05-23 | Codexis, Inc. | Biocatalysts for the preparation of hydroxy substituted carbamates |
US8504498B2 (en) | 2008-02-12 | 2013-08-06 | Codexis, Inc. | Method of generating an optimized, diverse population of variants |
AU2009212959B2 (en) * | 1999-06-14 | 2013-08-15 | Bp Corporation North America Inc. | Synthetic ligation reassembly in directed evolution |
WO2013142770A1 (en) | 2012-03-23 | 2013-09-26 | Codexis, Inc. | Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs |
WO2013166113A1 (en) | 2012-05-04 | 2013-11-07 | E. I. Du Pont De Nemours And Company | Compositions and methods comprising sequences having meganuclease activity |
WO2013170050A1 (en) | 2012-05-11 | 2013-11-14 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
WO2013169725A2 (en) | 2012-05-08 | 2013-11-14 | Codexis, Inc. | Biocatalysts and methods for hydroxylation of chemical compounds |
US8603949B2 (en) | 2003-06-17 | 2013-12-10 | California Institute Of Technology | Libraries of optimized cytochrome P450 enzymes and the optimized P450 enzymes |
WO2013188305A2 (en) | 2012-06-11 | 2013-12-19 | Codexis, Inc. | Fungal beta-xylosidase variants |
US8614081B2 (en) | 2009-07-23 | 2013-12-24 | Codexis, Inc. | Nitrilase biocatalysts |
EP2706119A1 (en) | 2009-11-25 | 2014-03-12 | Codexis, Inc. | Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass |
EP2706122A2 (en) | 2008-01-03 | 2014-03-12 | Verenium Corporation | Isomerases, nucleic acids encoding them and methods for making and using them |
US8709415B2 (en) | 2006-01-12 | 2014-04-29 | Alexion Pharmaceuticals, Inc. | Antibodies to OX-2/CD200 and uses thereof |
US8715996B2 (en) | 2009-02-26 | 2014-05-06 | Codexis, Inc. | Beta-glucosidase variant enzymes and related polynucleotides |
US8715962B2 (en) | 2010-03-31 | 2014-05-06 | Codexis, Inc. | Production of geranyl diphosphate |
WO2014099730A1 (en) | 2012-12-21 | 2014-06-26 | Codexis, Inc. | Engineered biocatalysts and methods for synthesizing chiral amines |
US8768871B2 (en) | 2008-02-12 | 2014-07-01 | Codexis, Inc. | Method of generating an optimized, diverse population of variants |
US8778652B2 (en) | 2011-06-30 | 2014-07-15 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
WO2014113521A1 (en) | 2013-01-18 | 2014-07-24 | Codexis, Inc. | Engineered biocatalysts useful for carbapenem synthesis |
WO2014120821A1 (en) | 2013-01-31 | 2014-08-07 | Codexis, Inc. | Methods, systems, and software for identifying bio-molecules using models of multiplicative form |
WO2014133960A1 (en) | 2013-02-28 | 2014-09-04 | Codexis, Inc. | Engineered transaminase polypeptides for industrial biocatalysis |
US8840885B2 (en) | 2000-12-08 | 2014-09-23 | Alexion Pharmaceuticals, Inc. | Methods for treating chronic lymphocytic leukemia |
WO2014153242A1 (en) | 2013-03-14 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Compositions having dicamba decarboxylase activity and methods of use |
WO2014153234A1 (en) | 2013-03-14 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Compositions having dicamba decarboxylase activity and methods of use |
WO2014150914A2 (en) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Phi-4 polypeptides and methods for their use |
WO2014172541A2 (en) | 2013-04-18 | 2014-10-23 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
WO2015023846A2 (en) | 2013-08-16 | 2015-02-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2015038734A2 (en) | 2013-09-13 | 2015-03-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US8986684B2 (en) | 2007-07-25 | 2015-03-24 | Alexion Pharmaceuticals, Inc. | Methods and compositions for treating autoimmune disease |
EP2853593A1 (en) | 2003-03-07 | 2015-04-01 | DSM IP Assets B.V. | Hydrolases, nucleic acids encoding them and mehods for making and using them |
US8999328B2 (en) | 2000-12-08 | 2015-04-07 | Alexion Pharmaceuticals, Inc. | Polypeptides and antibodies derived from chronic lymphocytic Leukemia cells and uses thereof |
US9006460B2 (en) | 2010-08-16 | 2015-04-14 | Cardiome International Ag | Process for preparing aminocyclohexyl ether compounds |
WO2015073555A1 (en) | 2013-11-13 | 2015-05-21 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
EP2886658A1 (en) | 2005-03-10 | 2015-06-24 | BASF Enzymes LLC | Lyase enzymes, nucleic acids encoding them and methods for making and using them |
US9080192B2 (en) | 2010-02-10 | 2015-07-14 | Codexis, Inc. | Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system |
WO2015120276A1 (en) | 2014-02-07 | 2015-08-13 | Pioneer Hi Bred International Inc | Insecticidal proteins and methods for their use |
WO2015120270A1 (en) | 2014-02-07 | 2015-08-13 | Pioneer Hi Bred International, Inc. | Insecticidal proteins and methods for their use |
CN104955961A (en) * | 2012-12-11 | 2015-09-30 | 塞勒密斯株式会社 | Method for synthesizing gene library using codon combination and mutagenesis |
US9150661B2 (en) | 2000-12-08 | 2015-10-06 | Alexion Pharmaceuticals, Inc. | Polypeptides and antibodies derived from chronic lymphocytic leukemia cells and uses thereof |
WO2015161019A1 (en) | 2014-04-16 | 2015-10-22 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
US9169468B2 (en) | 2011-03-30 | 2015-10-27 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
WO2016061206A1 (en) | 2014-10-16 | 2016-04-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US9322027B2 (en) | 2010-08-20 | 2016-04-26 | Shell Oil Company | Expression constructs comprising fungal promoters |
US9322007B2 (en) | 2011-07-22 | 2016-04-26 | The California Institute Of Technology | Stable fungal Cel6 enzyme variants |
WO2016085916A1 (en) | 2014-11-25 | 2016-06-02 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
WO2016105889A1 (en) | 2014-12-22 | 2016-06-30 | Codexis, Inc. | Human alpha-galactosidase variants |
WO2016130412A1 (en) | 2015-02-10 | 2016-08-18 | Codexis, Inc. | Ketoreductase polypeptides for the synthesis of chiral compounds |
US9434919B2 (en) | 2011-09-30 | 2016-09-06 | Codexis, Inc. | Fungal proteases |
WO2016144688A1 (en) | 2015-03-11 | 2016-09-15 | Pioneer Hi Bred International Inc | Insecticidal combinations of pip-72 and methods of use |
WO2016179464A1 (en) | 2015-05-07 | 2016-11-10 | Codexis, Inc. | Penicillin-g acylases |
WO2016186986A1 (en) | 2015-05-19 | 2016-11-24 | Pioneer Hi Bred International Inc | Insecticidal proteins and methods for their use |
EP3115459A2 (en) | 2008-05-23 | 2017-01-11 | E. I. du Pont de Nemours and Company | Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
WO2017007547A1 (en) | 2015-07-07 | 2017-01-12 | Codexis, Inc. | Novel p450-bm3 variants with improved activity |
WO2017023486A1 (en) | 2015-08-06 | 2017-02-09 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
US9611515B2 (en) | 2012-11-20 | 2017-04-04 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
US9611462B2 (en) | 2011-12-20 | 2017-04-04 | Codexis, Inc. | Endoglucanase 1B (EG1B) variants |
WO2017105987A1 (en) | 2015-12-18 | 2017-06-22 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3190182A1 (en) | 2004-03-08 | 2017-07-12 | DSM IP Assets B.V. | Phospholipases, nucleic acids encoding them and methods for making and using them |
US9708587B2 (en) | 2014-07-09 | 2017-07-18 | Codexis, Inc. | P450-BM3 variants with improved activity |
US9752171B2 (en) | 2010-05-28 | 2017-09-05 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
WO2017193022A1 (en) | 2016-05-05 | 2017-11-09 | Codexis, Inc. | Penicillin-g acylases |
WO2017192560A1 (en) | 2016-05-04 | 2017-11-09 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2017213758A1 (en) | 2016-06-09 | 2017-12-14 | Codexis, Inc. | Biocatalysts and methods for hydroxylation of chemical compounds |
WO2018005411A1 (en) | 2016-07-01 | 2018-01-04 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
WO2018038906A1 (en) | 2016-08-26 | 2018-03-01 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
EP3301208A1 (en) | 2009-09-18 | 2018-04-04 | Codexis, Inc. | Reduced codon mutagenesis |
WO2018084936A1 (en) | 2016-11-01 | 2018-05-11 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2018111551A1 (en) | 2016-12-14 | 2018-06-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2018118811A1 (en) | 2016-12-22 | 2018-06-28 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2018144679A2 (en) | 2017-02-03 | 2018-08-09 | Codexis, Inc. | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
WO2018148001A1 (en) | 2017-02-08 | 2018-08-16 | Pioneer Hi-Bred International Inc | Insecticidal combinations of plant derived insecticidal proteins and methods for their use |
WO2018208665A1 (en) | 2017-05-08 | 2018-11-15 | Codexis, Inc. | Engineered ligase variants |
WO2018208882A1 (en) | 2017-05-11 | 2018-11-15 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3406594A1 (en) | 2012-12-07 | 2018-11-28 | Merck Sharp & Dohme Corp. | Biocatalytic transamination process |
EP3418929A1 (en) | 2013-09-27 | 2018-12-26 | Codexis, Inc. | Automated screening of enzyme variants |
WO2019040335A1 (en) | 2017-08-19 | 2019-02-28 | University Of Rochester | Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents |
EP3450549A1 (en) | 2012-10-29 | 2019-03-06 | University Of Rochester | Artemisinin derivatives, methods for their preparation and their use as antimalarial agents |
EP3460061A1 (en) | 2008-09-26 | 2019-03-27 | Tocagen Inc. | Recombinant replication competent murine leukemia gamma-retrovirus (mlv) for the expression of heat-stabilized cytosine deaminase |
WO2019116311A1 (en) | 2017-12-13 | 2019-06-20 | Glaxosmithkline Intellectual Property Development Limited | Carboxyesterase biocatalysts |
US10738286B2 (en) | 2017-06-30 | 2020-08-11 | Codexis, Inc. | T7 RNA polymerase variants |
US10745681B2 (en) | 2017-01-05 | 2020-08-18 | Codexis, Inc. | Penicillin-G acylases |
US10793841B2 (en) | 2017-06-30 | 2020-10-06 | Codexis, Inc. | T7 RNA polymerase variants |
US10889806B2 (en) | 2018-07-09 | 2021-01-12 | Codexis, Inc. | Engineered pantothenate kinase variant enzymes |
US10900055B2 (en) | 2018-06-12 | 2021-01-26 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
WO2021067608A1 (en) | 2019-10-02 | 2021-04-08 | Abbott Laboratories | Detection of analytes by protein switches |
US10995329B2 (en) | 2017-02-13 | 2021-05-04 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US11015180B2 (en) | 2017-12-13 | 2021-05-25 | Codexis, Inc. | Carboxyesterase polypeptides for amide coupling |
US11021729B2 (en) | 2017-04-27 | 2021-06-01 | Codexis, Inc. | Ketoreductase polypeptides and polynucleotides |
US11034948B2 (en) | 2018-07-09 | 2021-06-15 | Codexis, Inc. | Engineered phosphopentomutase variant enzymes |
US11060075B2 (en) | 2018-10-29 | 2021-07-13 | Codexis, Inc. | Engineered DNA polymerase variants |
US11161834B2 (en) | 2017-04-24 | 2021-11-02 | Tesaro, Inc. | Methods of manufacturing of niraparib |
US11162105B2 (en) | 2018-07-09 | 2021-11-02 | Codexis, Inc. | Engineered purine nucleoside phosphorylase variant enzymes |
US11198861B2 (en) | 2018-07-12 | 2021-12-14 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US11214817B2 (en) | 2005-03-28 | 2022-01-04 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
WO2022015619A2 (en) | 2020-07-14 | 2022-01-20 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US11274286B2 (en) | 2018-07-09 | 2022-03-15 | Codexis, Inc. | Engineered deoxyribose-phosphate aldolases |
US11299723B2 (en) | 2016-06-15 | 2022-04-12 | Codexis, Inc. | Engineered beta-glucosidases and glucosylation methods |
US11319531B2 (en) | 2017-11-07 | 2022-05-03 | Codexis, Inc. | Transglutaminase variants |
US11359183B2 (en) | 2017-06-14 | 2022-06-14 | Codexis, Inc. | Engineered transaminase polypeptides for industrial biocatalysis |
WO2022125960A1 (en) | 2020-12-11 | 2022-06-16 | Willow Biosciences, Inc. | Recombinant acyl activating enzyme (aae) genes for enhanced biosynthesis of cannabinoids and cannabinoid precursors |
US11427813B2 (en) | 2018-12-20 | 2022-08-30 | Codexis, Inc. | Human alpha-galactosidase variants |
WO2022212835A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered acetate kinase variant enzymes |
WO2022212824A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered adenylate kinase variant enzymes |
WO2022212832A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered cyclic gmp-amp synthase (cgas) variant enzymes |
WO2022212828A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered guanylate kinase variant enzymes |
US11466259B2 (en) | 2018-07-09 | 2022-10-11 | Codexis, Inc. | Engineered galactose oxidase variant enzymes |
US11473077B2 (en) | 2018-12-14 | 2022-10-18 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
EP4122945A1 (en) | 2013-12-23 | 2023-01-25 | University of Rochester | Methods and compositions for ribosomal synthesis of macrocyclic peptides |
WO2023010083A2 (en) | 2021-07-30 | 2023-02-02 | Willow Biosciences, Inc. | Recombinant prenyltransferase polypeptides engineered for enhanced biosynthesis of cannabinoids |
WO2023023621A1 (en) | 2021-08-19 | 2023-02-23 | Willow Biosciences, Inc. | Recombinant olivetolic acid cyclase polypeptides engineered for enhanced biosynthesis of cannabinoids |
WO2023069921A1 (en) | 2021-10-19 | 2023-04-27 | Epimeron Usa, Inc. | Recombinant thca synthase polypeptides engineered for enhanced biosynthesis of cannabinoids |
US11643642B2 (en) | 2017-06-27 | 2023-05-09 | Codexis, Inc. | Penicillin-g acylases |
WO2023173084A1 (en) | 2022-03-11 | 2023-09-14 | University Of Rochester | Cyclopeptibodies and uses thereof |
US11761963B2 (en) | 2017-09-27 | 2023-09-19 | Alexion Pharmaceuticals, Inc. | Biomarker signature for predicting tumor response to anti-CD200 therapy |
US11760981B2 (en) | 2018-07-30 | 2023-09-19 | Codexis, Inc. | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
US11767519B2 (en) | 2020-08-28 | 2023-09-26 | Codexis, Inc. | Engineered amylase variants |
US11788071B2 (en) | 2020-04-10 | 2023-10-17 | Codexis, Inc. | Engineered transaminase polypeptides |
US11898174B2 (en) | 2020-12-18 | 2024-02-13 | Codexis, Inc. | Engineered uridine phosphorylase variant enzymes |
WO2024040020A1 (en) | 2022-08-15 | 2024-02-22 | Absci Corporation | Quantitative affinity activity specific cell enrichment |
US11959108B2 (en) | 2020-08-28 | 2024-04-16 | Codexis, Inc. | Engineered protease variants |
US11970722B2 (en) | 2020-12-18 | 2024-04-30 | Codexis, Inc. | Engineered acid alpha-glucosidase variants |
Families Citing this family (162)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6406855B1 (en) | 1994-02-17 | 2002-06-18 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
GB9722131D0 (en) * | 1997-10-20 | 1997-12-17 | Medical Res Council | Method |
US6759243B2 (en) * | 1998-01-20 | 2004-07-06 | Board Of Trustees Of The University Of Illinois | High affinity TCR proteins and methods |
JP2002502977A (en) | 1998-02-04 | 2002-01-29 | インビトロジェン コーポレイション | Microarrays and their uses |
US7390619B1 (en) * | 1998-02-11 | 2008-06-24 | Maxygen, Inc. | Optimization of immunomodulatory properties of genetic vaccines |
EP1073770B1 (en) * | 1998-04-30 | 2004-11-17 | Max-Planck-Gesellschaft Zur Förderung Der Wissenschaften E.V. | Novel method for the identification of clones conferring a desired biological property from an expression library |
US7153655B2 (en) * | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
US6846655B1 (en) | 1998-06-29 | 2005-01-25 | Phylos, Inc. | Methods for generating highly diverse libraries |
US20030104417A1 (en) * | 1998-08-12 | 2003-06-05 | Proteus S.A. | Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides |
US20030092023A1 (en) * | 1998-08-12 | 2003-05-15 | Daniel Dupret | Method of shuffling polynucleotides using templates |
US20040191772A1 (en) * | 1998-08-12 | 2004-09-30 | Dupret Daniel Marc | Method of shuffling polynucleotides using templates |
US20040005673A1 (en) * | 2001-06-29 | 2004-01-08 | Kevin Jarrell | System for manipulating nucleic acids |
ATE439437T1 (en) * | 1999-01-05 | 2009-08-15 | Univ Boston | IMPROVED CLONING PROCESS |
AU2415200A (en) * | 1999-01-18 | 2000-08-01 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
US20030054390A1 (en) * | 1999-01-19 | 2003-03-20 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US20070065838A1 (en) * | 1999-01-19 | 2007-03-22 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US20090130718A1 (en) * | 1999-02-04 | 2009-05-21 | Diversa Corporation | Gene site saturation mutagenesis |
AU5557400A (en) * | 1999-03-18 | 2001-01-31 | Complete Genomics As | Methods of cloning and producing fragment chains with readable information content |
US20040002474A1 (en) * | 1999-10-07 | 2004-01-01 | Maxygen Inc. | IFN-alpha homologues |
CN1425135A (en) * | 1999-12-27 | 2003-06-18 | 东丽株式会社 | Cytokine-like peptide |
US20010039014A1 (en) * | 2000-01-11 | 2001-11-08 | Maxygen, Inc. | Integrated systems and methods for diversity generation and screening |
AU5951201A (en) | 2000-05-04 | 2001-11-12 | Univ Yale | High density protein arrays for screening of protein activity |
US7846733B2 (en) * | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
WO2002008408A2 (en) * | 2000-07-21 | 2002-01-31 | Trustees Of Boston University | Modular vector systems |
US7435562B2 (en) * | 2000-07-21 | 2008-10-14 | Modular Genetics, Inc. | Modular vector systems |
AUPQ974900A0 (en) * | 2000-08-29 | 2000-09-21 | Macquarie Research Limited | Degenerate oligonucleotide gene-shuffling |
US6958213B2 (en) * | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
US6858413B2 (en) | 2000-12-13 | 2005-02-22 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US20020086292A1 (en) * | 2000-12-22 | 2002-07-04 | Shigeaki Harayama | Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules |
US20030036641A1 (en) * | 2001-01-31 | 2003-02-20 | Padgett Hal S. | Methods for homology-driven reassembly of nucleic acid sequences |
US20110111413A1 (en) * | 2001-02-02 | 2011-05-12 | Padgett Hal S | Method of optimizing codon usage through dna shuffling |
KR20040010606A (en) * | 2001-03-07 | 2004-01-31 | 제노젠 코퍼레이션 | Methods of screening for introduction of dna into a target cell |
BR0205268A (en) | 2001-03-09 | 2004-11-30 | Nugen Technologies Inc | Processes and compositions for mRNA sequence mplification |
WO2002074978A2 (en) | 2001-03-19 | 2002-09-26 | President And Fellows Of Harvard College | Nucleic acid shuffling |
US7807408B2 (en) * | 2001-03-19 | 2010-10-05 | President & Fellows Of Harvard College | Directed evolution of proteins |
JP2005515468A (en) * | 2001-08-20 | 2005-05-26 | リジェネシス バイオリメディエイション プロダクツ | Biosensors for analytes of small molecules |
WO2003035842A2 (en) * | 2001-10-24 | 2003-05-01 | Dyax Corporation | Hybridization control of sequence variation |
US20040009498A1 (en) * | 2002-01-14 | 2004-01-15 | Diversa Corporation | Chimeric antigen binding molecules and methods for making and using them |
US20050075298A1 (en) * | 2002-01-31 | 2005-04-07 | Wei Chen | Methods and composition for delivering nucleic acids and/or proteins to the intestinal mucosa |
US20040043003A1 (en) * | 2002-01-31 | 2004-03-04 | Wei Chen | Clinical grade vectors based on natural microflora for use in delivering therapeutic compositions |
US20040009937A1 (en) * | 2002-01-31 | 2004-01-15 | Wei Chen | Methods and composition for delivering nucleic acids and/or proteins to the respiratory system |
WO2003072054A2 (en) * | 2002-02-25 | 2003-09-04 | Cabot Corporation | Custom ligand design for biomolecular filtration and purification for bioseperation |
US20050084907A1 (en) | 2002-03-01 | 2005-04-21 | Maxygen, Inc. | Methods, systems, and software for identifying functional biomolecules |
US7747391B2 (en) * | 2002-03-01 | 2010-06-29 | Maxygen, Inc. | Methods, systems, and software for identifying functional biomolecules |
EP1488001B1 (en) * | 2002-03-11 | 2008-08-20 | Nugen Technologies, Inc. | Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination |
CA2485506C (en) * | 2002-05-17 | 2012-02-28 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
CA2498319A1 (en) * | 2002-09-09 | 2004-03-18 | Nautilus Biotech | Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules |
US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
MXPA05003493A (en) * | 2002-10-02 | 2005-09-30 | Catalyst Biosciences | Methods of generating and screenign for porteases with altered specificity. |
US20060024289A1 (en) * | 2002-10-02 | 2006-02-02 | Ruggles Sandra W | Cleavage of VEGF and VEGF receptor by wild-type and mutant proteases |
EP1551876B1 (en) | 2002-10-16 | 2011-03-16 | Purdue Pharma L.P. | Antibodies that bind cell-associated ca 125/0722p and methods of use thereof |
US7314613B2 (en) * | 2002-11-18 | 2008-01-01 | Maxygen, Inc. | Interferon-alpha polypeptides and conjugates |
KR20050086498A (en) * | 2002-11-18 | 2005-08-30 | 맥시겐, 인크. | Interferon-alpha polypeptides and conjugates |
US20040121305A1 (en) * | 2002-12-18 | 2004-06-24 | Wiegand Roger Charles | Generation of efficacy, toxicity and disease signatures and methods of use thereof |
CA2521084A1 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
WO2005047461A2 (en) * | 2003-08-18 | 2005-05-26 | The Regents Of The University Of California | Polypeptide display libraries and methods of making and using thereof |
ATE503834T1 (en) * | 2003-08-27 | 2011-04-15 | Proterec Ltd | LIBRARIES OF RECOMBINANT CHIMERIC PROTEINS |
AU2004286265A1 (en) * | 2003-10-29 | 2005-05-12 | Ribomed Biotechnologies, Inc. | Compositions, methods and detection technologies for reiterative oligonucleotide synthesis |
ES2640284T3 (en) * | 2004-04-12 | 2017-11-02 | Catalyst Biosciences, Inc. | MT-SP1 mutant polypeptides |
CN101115769A (en) * | 2004-05-19 | 2008-01-30 | 马克西根公司 | Interferon-alpha polypeptides and conjugates |
WO2006004959A2 (en) * | 2004-06-30 | 2006-01-12 | Egen Corporation | Pegylated interferon alpha-1b |
GB0422052D0 (en) | 2004-10-04 | 2004-11-03 | Dansico As | Enzymes |
US20070269870A1 (en) * | 2004-10-18 | 2007-11-22 | George Church | Methods for assembly of high fidelity synthetic polynucleotides |
US20070122817A1 (en) * | 2005-02-28 | 2007-05-31 | George Church | Methods for assembly of high fidelity synthetic polynucleotides |
GB0423139D0 (en) | 2004-10-18 | 2004-11-17 | Danisco | Enzymes |
CN101094867B (en) | 2004-10-19 | 2011-08-24 | 隆萨股份公司 | Method for solid phase peptide synthesis |
CA2590245A1 (en) * | 2004-11-11 | 2006-05-18 | Modular Genetics, Inc. | Ladder assembly and system for generating diversity |
WO2006062971A2 (en) * | 2004-12-08 | 2006-06-15 | Ceres Inc. | Modulating plant carbon levels |
US7335510B2 (en) * | 2004-12-16 | 2008-02-26 | Ceres, Inc. | Modulating plant nitrogen levels |
CA2594832A1 (en) * | 2005-01-13 | 2006-07-20 | Codon Devices, Inc. | Compositions and methods for protein design |
WO2006127423A2 (en) * | 2005-05-18 | 2006-11-30 | Codon Devices, Inc. | Methods of producing polynucleotide libraries using scarless ligation |
JP2008545393A (en) * | 2005-05-18 | 2008-12-18 | マキシジェン, インコーポレイテッド | Advanced interferon alpha polypeptide |
PT2314623E (en) | 2005-06-21 | 2012-10-02 | Xoma Technology Ltd | Il-1beta binding antibodies and fragments thereof |
JP2009501522A (en) * | 2005-07-12 | 2009-01-22 | コドン デバイシズ インコーポレイテッド | Compositions and methods for biocatalytic engineering |
WO2007008951A1 (en) * | 2005-07-12 | 2007-01-18 | Codon Devices, Inc. | Compositions and methods for design of non-immunogenic proteins |
EP1919931A4 (en) | 2005-08-31 | 2010-01-20 | Univ California | Cellular libraries of peptide sequences (clips) and methods of using the same |
WO2007030759A2 (en) | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
GB2432366B (en) * | 2005-11-19 | 2007-11-21 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
KR100759817B1 (en) * | 2005-12-08 | 2007-09-20 | 한국전자통신연구원 | Method and device for predicting regulation of multiple transcription factors |
US20090304901A1 (en) * | 2006-01-25 | 2009-12-10 | Steven Craig Bobzin | Modulating plant protein levels |
US8222482B2 (en) * | 2006-01-26 | 2012-07-17 | Ceres, Inc. | Modulating plant oil levels |
US7935342B2 (en) | 2006-02-02 | 2011-05-03 | Rinat Neuroscience Corp. | Methods for treating obesity by administering a trkB antagonist |
US20070231805A1 (en) * | 2006-03-31 | 2007-10-04 | Baynes Brian M | Nucleic acid assembly optimization using clamped mismatch binding proteins |
US20100024070A1 (en) * | 2006-05-15 | 2010-01-28 | Steven Craig Bobzin | Modulation of oil levels in plants |
WO2007136834A2 (en) * | 2006-05-19 | 2007-11-29 | Codon Devices, Inc. | Combined extension and ligation for nucleic acid assembly |
AR061365A1 (en) * | 2006-06-13 | 2008-08-20 | Athenix Corp | METHODS TO GENERATE GENETIC DIVERSITY THROUGH MUTAGENESIS WITH PERMUTATIONS |
US20090320165A1 (en) * | 2006-06-21 | 2009-12-24 | Steven Craig Bobzin | Modulation of protein levels in plants |
US7727745B2 (en) * | 2006-08-14 | 2010-06-01 | Bio-Id Diagnostic Inc. | Synthesis of single-stranded DNA |
AU2007234569C1 (en) * | 2006-08-15 | 2008-05-29 | Commonwealth Scientific And Industrial Research Organisation | Reassortment by fragment ligation |
US8158411B2 (en) | 2006-08-21 | 2012-04-17 | Samsung Electronics Co., Ltd. | Method of separating microorganism using nonplanar solid substrate and device for separating microorganism using the same |
US8053191B2 (en) | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
US20100151109A1 (en) * | 2006-12-15 | 2010-06-17 | Amr Saad Ragab | Modulation of plant protein levels |
US20080175668A1 (en) * | 2007-01-18 | 2008-07-24 | Larry Wayne Haese | Graywater recycling system including rainwater recovery |
US8143046B2 (en) | 2007-02-07 | 2012-03-27 | Danisco Us Inc., Genencor Division | Variant Buttiauxella sp. phytases having altered properties |
US20090118130A1 (en) | 2007-02-12 | 2009-05-07 | Codexis, Inc. | Structure-activity relationships |
EP2126105A4 (en) * | 2007-02-20 | 2010-11-03 | Anaptysbio Inc | Somatic hypermutation systems |
US7625555B2 (en) * | 2007-06-18 | 2009-12-01 | Novagen Holding Corporation | Recombinant human interferon-like proteins |
WO2009014726A1 (en) | 2007-07-26 | 2009-01-29 | The Regents Of The University Of California | Methods for enhancing bacterial cell display of proteins and peptides |
ES2701883T3 (en) * | 2007-07-31 | 2019-02-26 | Basf Enzymes Llc | Assembling custom-made multiple site combinations |
US20110166027A1 (en) * | 2007-08-21 | 2011-07-07 | Affomix Corporation | Interaction screening methods, systems and devices |
WO2009046153A1 (en) | 2007-10-01 | 2009-04-09 | Codexis, Inc. | Ketoreductase polypeptides for the production of azetidinone |
WO2009097673A1 (en) * | 2008-02-07 | 2009-08-13 | Bio-Id Diagnostic Inc. | Synthesis of single-stranded dna |
US20090203531A1 (en) * | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
CA3224206A1 (en) | 2008-02-15 | 2009-08-20 | Ceres, Inc. | Drought and heat tolerance in plants |
WO2009117698A2 (en) | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
WO2010054319A2 (en) | 2008-11-10 | 2010-05-14 | Codexis, Inc. | Penicillin-g acylases |
SG172783A1 (en) | 2008-12-25 | 2011-08-29 | Codexis Inc | Enone reductases |
EP2393944A4 (en) * | 2009-02-03 | 2013-11-27 | Complete Genomics Inc | Indexing a reference sequence for oligomer sequence mapping |
US8615365B2 (en) * | 2009-02-03 | 2013-12-24 | Complete Genomics, Inc. | Oligomer sequences mapping |
US8731843B2 (en) * | 2009-02-03 | 2014-05-20 | Complete Genomics, Inc. | Oligomer sequences mapping |
JP5707344B2 (en) | 2009-02-26 | 2015-04-30 | コデクシス, インコーポレイテッド | Transaminase biocatalyst |
EP2406399B1 (en) * | 2009-03-09 | 2018-02-14 | Bioatla, LLC | Mirac proteins |
WO2010123625A1 (en) | 2009-04-24 | 2010-10-28 | University Of Southern California | Cd133 polymorphisms predict clinical outcome in patients with cancer |
EP2511843B1 (en) * | 2009-04-29 | 2016-12-21 | Complete Genomics, Inc. | Method and system for calling variations in a sample polynucleotide sequence with respect to a reference polynucleotide sequence |
CA2765715A1 (en) | 2009-06-16 | 2010-12-23 | Codexis, Inc. | .beta.-glucosidase variants |
EP2446026B1 (en) | 2009-06-22 | 2017-08-09 | Codexis, Inc. | Transaminase reactions |
ES2538396T3 (en) | 2009-09-30 | 2015-06-19 | Codexis, Inc. | Recombinant C1-glucosidase for the production of sugars from cellulosic biomass |
WO2011063080A2 (en) | 2009-11-20 | 2011-05-26 | Codexis, Inc. | Mutli-cellulase enzyme compositions for hydrolysis of cellulosic biomass |
WO2011084757A1 (en) | 2009-12-21 | 2011-07-14 | University Of Southern California | Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer |
WO2011085334A1 (en) | 2010-01-11 | 2011-07-14 | University Of Southern California | Cd44 polymorphisms predict clinical outcome in patients with gastric cancer |
WO2011143632A2 (en) | 2010-05-14 | 2011-11-17 | Codexis, Inc. | Cellobiohydrolase variants |
US8932836B2 (en) | 2010-08-16 | 2015-01-13 | Codexis, Inc. | Biocatalysts and methods for the synthesis of (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine |
HUE028981T2 (en) | 2010-11-02 | 2017-02-28 | Codexis Inc | Compositions and methods for production of fermentable sugars |
BR112013010813A2 (en) | 2010-11-02 | 2016-07-12 | Codexis Inc | fungal cells, mixed enzymes, methods for glucose generation and fermentation medium. |
US10457935B2 (en) | 2010-11-12 | 2019-10-29 | Gen9, Inc. | Protein arrays and methods of using and making the same |
IL280133B2 (en) | 2010-11-12 | 2023-04-01 | Gen9 Inc | Methods and devices for nucleic acids synthesis |
US8906689B2 (en) | 2010-12-21 | 2014-12-09 | Codexis, Inc. | Endoglucanase variants |
US20120258866A1 (en) * | 2011-04-05 | 2012-10-11 | Full Spectrum Genetics, Inc. | Multi-dimensional selection of protein mutants using high throughput sequence analysis |
JP6243325B2 (en) | 2011-04-12 | 2017-12-06 | ガンガゲン インコーポレーティッド | Chimeric antimicrobial polypeptide |
EP2726651B1 (en) | 2011-06-28 | 2018-11-07 | Codexis, Inc. | Protein variant generation by region shuffling |
BR112014003740A2 (en) | 2011-08-23 | 2018-08-14 | Codexis Inc | cellobiohydrolase variants |
ES2737957T3 (en) | 2011-08-26 | 2020-01-17 | Gen9 Inc | Compositions and methods for high fidelity nucleic acid assembly |
WO2013096092A1 (en) | 2011-12-20 | 2013-06-27 | Codexis, Inc. | Production of saturated fatty alcohols from engineered microorganisms |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
US20150133698A1 (en) | 2012-04-20 | 2015-05-14 | Codexis, Inc. | Production of fatty alcohols from engineered microorganisms |
CN104603286B (en) | 2012-04-24 | 2020-07-31 | Gen9股份有限公司 | Method for sorting nucleic acids and multiplex preparations in vitro cloning |
CA2873511A1 (en) | 2012-05-17 | 2013-11-21 | Ra Pharmaceuticals, Inc. | Peptide and peptidomimetic inhibitors |
LT2864531T (en) | 2012-06-25 | 2019-03-12 | Gen9, Inc. | Methods for nucleic acid assembly and high throughput sequencing |
US9650655B2 (en) | 2012-07-20 | 2017-05-16 | Codexis, Inc. | Production of fatty alcohols from engineered microorganisms |
WO2014025747A1 (en) | 2012-08-07 | 2014-02-13 | Codexis, Inc. | Glucose and xylose co-utilization in e. coli |
WO2014093070A1 (en) | 2012-12-14 | 2014-06-19 | Codexis, Inc. | Modified native beta-ketoacyl-acp synthases and engineered microorganisms |
US9670493B2 (en) | 2013-03-14 | 2017-06-06 | Codexis, Inc. | Low-phosphate repressible promoter |
WO2015027245A1 (en) | 2013-08-23 | 2015-02-26 | Complete Genomics, Inc. | Long fragment de novo assembly using short reads |
WO2015145463A1 (en) | 2014-03-27 | 2015-10-01 | Gangagen, Inc. | Phage-derived compositions for improved mycobacterial therapy |
CA2955984A1 (en) | 2014-07-22 | 2016-01-28 | The University Of Notre Dame Du Lac | Molecular constructs and uses thereof |
US10045987B2 (en) | 2014-08-28 | 2018-08-14 | Codexis, Inc. | Imidazoyl anilide derivatives and methods of use |
US10273229B2 (en) | 2014-08-28 | 2019-04-30 | Codexis, Inc. | N-substituted 4-aminoquinazoline derivatives and methods of use |
WO2016183532A1 (en) | 2015-05-13 | 2016-11-17 | Synlogic, Inc. | Bacteria engineered to treat a disease or disorder |
US11291693B2 (en) | 2015-06-25 | 2022-04-05 | Synlogic Operating Company, Inc. | Bacteria engineered to treat metabolic diseases |
BR112018011503A2 (en) | 2015-12-07 | 2018-12-04 | Zymergen Inc | corynebacterium glutamicum promoters |
US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
WO2017123592A1 (en) | 2016-01-11 | 2017-07-20 | Synlogic, Inc. | Bacteria engineered to treat disorders associated with bile salts |
WO2017123676A1 (en) | 2016-01-11 | 2017-07-20 | Synlogic, Inc. | Recombinant bacteria engineered to treat diseases and disorders associated with amino acid metabolism and methods of use thereof |
EP3402497A1 (en) | 2016-01-11 | 2018-11-21 | Synlogic Operating Company, Inc. | Bacteria engineered to treat metabolic diseases |
WO2017139708A1 (en) | 2016-02-10 | 2017-08-17 | Synlogic, Inc. | Bacteria engineered to treat nonalcoholic steatohepatitis (nash) |
JP2019519241A (en) | 2016-06-30 | 2019-07-11 | ザイマージェン インコーポレイテッド | Method for producing a glucose permease library and its use |
WO2018005655A2 (en) | 2016-06-30 | 2018-01-04 | Zymergen Inc. | Methods for generating a bacterial hemoglobin library and uses thereof |
WO2023245171A1 (en) | 2022-06-17 | 2023-12-21 | Synlogic Operating Company, Inc. | Bacteria engineered to treat diseases associated with bile acid metabolism and methods of use thereof |
WO2023245168A1 (en) | 2022-06-17 | 2023-12-21 | Synlogic Operating Company, Inc. | Bacteria engineered to treat diseases associated with bile acid metabolism and methods of use thereof |
WO2023250478A1 (en) | 2022-06-23 | 2023-12-28 | Synlogic Operating Company, Inc. | Recombinant bacteria engineered to treat diseases associated with methionine metabolism and methods of use thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2183661A (en) * | 1985-03-30 | 1987-06-10 | Marc Ballivet | Method for obtaining dna, rna, peptides, polypeptides or proteins by means of a dna recombinant technique |
WO1989006694A1 (en) * | 1988-01-15 | 1989-07-27 | Trustees Of The University Of Pennsylvania | Process for selection of proteinaceous substances which mimic growth-inducing molecules |
WO1991000925A1 (en) * | 1989-07-13 | 1991-01-24 | Massachusetts Institute Of Technology | Determination of a mutational spectrum |
US5525486A (en) * | 1993-01-14 | 1996-06-11 | Tasuku Honjo | Process for constructing cDNA library, and novel polypeptide and DNA coding for the same |
US5565332A (en) * | 1991-09-23 | 1996-10-15 | Medical Research Council | Production of chimeric antibodies - a combinatorial approach |
Family Cites Families (184)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL154598B (en) | 1970-11-10 | 1977-09-15 | Organon Nv | PROCEDURE FOR DETERMINING AND DETERMINING LOW MOLECULAR COMPOUNDS AND PROTEINS THAT CAN SPECIFICALLY BIND THESE COMPOUNDS AND TEST PACKAGING. |
US3817837A (en) | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
US3939350A (en) | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
US3996945A (en) * | 1975-04-07 | 1976-12-14 | Mcdowell James A | Extinguisher for cigarettes or cigars |
US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
US4277437A (en) | 1978-04-05 | 1981-07-07 | Syva Company | Kit for carrying out chemically induced fluorescence immunoassay |
US4351901A (en) * | 1980-03-24 | 1982-09-28 | Cetus Corporation | Method for single nucleotide alteration |
US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
US4695543A (en) * | 1982-03-23 | 1987-09-22 | Bristol-Myers Company | Alpha Interferon GX-1 |
US5618697A (en) | 1982-12-10 | 1997-04-08 | Novo Nordisk A/S | Process for preparing a desired protein |
US4994379A (en) | 1983-03-09 | 1991-02-19 | Cetus Corporation | Modified signal peptides |
US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
US5538868A (en) | 1984-02-08 | 1996-07-23 | Cetus Oncology Corporation | Recombinant ricin toxin fragments |
US5422266A (en) * | 1984-12-31 | 1995-06-06 | University Of Georgia Research Foundation, Inc. | Recombinant DNA vectors capable of expressing apoaequorin |
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US5176995A (en) | 1985-03-28 | 1993-01-05 | Hoffmann-La Roche Inc. | Detection of viruses by amplification and hybridization |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
EP0316018A3 (en) * | 1985-03-29 | 1989-07-26 | Cetus Oncology Corporation | Modification of dna sequences |
US6492107B1 (en) | 1986-11-20 | 2002-12-10 | Stuart Kauffman | Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique |
US4959312A (en) | 1985-05-31 | 1990-09-25 | The University Of Tennessee Research Corporation | Full spectrum mutagenesis |
US4766106A (en) | 1985-06-26 | 1988-08-23 | Cetus Corporation | Solubilization of proteins for pharmaceutical compositions using polymer conjugation |
ATE93541T1 (en) | 1985-07-03 | 1993-09-15 | Genencor Int | HYBRID POLYPEPTIDES AND PROCESS FOR THEIR PRODUCTION. |
US5866363A (en) | 1985-08-28 | 1999-02-02 | Pieczenik; George | Method and means for sorting and identifying biological information |
US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
FI72765C (en) | 1986-02-13 | 1987-07-10 | Valmet Oy | UTBREDNINGSVALS ELLER MOTSVARANDE FOER VAEVNADEN I EN PAPPERSMASKIN SAMT FOERFARANDE FOER FRAMSTAELLNING AV DENNA. |
DK311186D0 (en) | 1986-06-30 | 1986-06-30 | Novo Industri As | ENZYMES |
US5824469A (en) | 1986-07-17 | 1998-10-20 | University Of Washington | Method for producing novel DNA sequences with biological activity |
US4795699A (en) | 1987-01-14 | 1989-01-03 | President And Fellows Of Harvard College | T7 DNA polymerase |
US4994368A (en) | 1987-07-23 | 1991-02-19 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
DK6488D0 (en) | 1988-01-07 | 1988-01-07 | Novo Industri As | ENZYMES |
US5206152A (en) * | 1988-04-08 | 1993-04-27 | Arch Development Corporation | Cloning and expression of early growth regulatory protein genes |
US4994366A (en) * | 1988-04-28 | 1991-02-19 | Jac Creative Foods, Inc. | Shrimp analog forming process |
JPH03505966A (en) * | 1988-07-14 | 1991-12-26 | ベイラー カレッジ オブ メディシン | Solid-phase assembly and reassembly method for biopolymers |
US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
FR2641793B1 (en) | 1988-12-26 | 1993-10-01 | Setratech | METHOD OF IN VIVO RECOMBINATION OF DNA SEQUENCES HAVING BASIC MATCHING |
US4902502A (en) | 1989-01-23 | 1990-02-20 | Cetus Corporation | Preparation of a polymer/interleukin-2 conjugate |
DD282028A5 (en) | 1989-02-16 | 1990-08-29 | Akad Wissenschaften Ddr | PROCESS FOR PREPARING THERMOSTABILES, HYBRIDEN BACILLUS BETA-1,3-1,4-GLUCANASE |
ATE143696T1 (en) * | 1989-02-28 | 1996-10-15 | Canon Kk | PARTIALLY DOUBLE STRANDED OLIGONUCLEOTIDE AND METHOD FOR FORMING IT |
DE3909710A1 (en) * | 1989-03-23 | 1990-09-27 | Boehringer Mannheim Gmbh | METHOD FOR EXPRESSING A RECOMBINANT GENE |
US5106727A (en) | 1989-04-27 | 1992-04-21 | Life Technologies, Inc. | Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers |
US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
JPH0372882A (en) | 1989-05-10 | 1991-03-28 | Eastman Kodak Co | Sequential configuration of synthetic two-chain dna |
US5556750A (en) | 1989-05-12 | 1996-09-17 | Duke University | Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems |
US6291158B1 (en) | 1989-05-16 | 2001-09-18 | Scripps Research Institute | Method for tapping the immunological repertoire |
CA2016841C (en) | 1989-05-16 | 1999-09-21 | William D. Huse | A method for producing polymers having a preselected activity |
CA2016842A1 (en) | 1989-05-16 | 1990-11-16 | Richard A. Lerner | Method for tapping the immunological repertoire |
US6291161B1 (en) | 1989-05-16 | 2001-09-18 | Scripps Research Institute | Method for tapping the immunological repertiore |
DK169306B1 (en) | 1989-06-14 | 1994-10-10 | Af 18 Juni 1990 As | Means for removing or inactivating unwanted components in blood or other extracellular body fluids and method of preparing dextran-O-carbonyl-benzo-18-crown-6 and of dextran-hydroxyethylbenzo-18-crown-6 |
DK0405858T3 (en) | 1989-06-26 | 1995-09-04 | Lilly Co Eli | Control of accumulation of aberrant expression vectors during fermentation procedures |
US5240845A (en) * | 1989-07-11 | 1993-08-31 | Otsuka Pharmaceutical Factory, Ltd. | Mutated streptokinase proteins |
IT1231157B (en) | 1989-07-20 | 1991-11-19 | Crc Ricerca Chim | NEW FUNCTIONAL HYBRID GENES OF BACILLUS THURINGIENSIS OBTAINED THROUGH IN VIVO RECOMBINATION. |
US5514787A (en) * | 1989-07-21 | 1996-05-07 | Washington University | DNA sequences encoding human membrane cofactor protein (MCP) |
US5574205A (en) | 1989-07-25 | 1996-11-12 | Cell Genesys | Homologous recombination for universal donor cells and chimeric mammalian hosts |
US5023171A (en) | 1989-08-10 | 1991-06-11 | Mayo Foundation For Medical Education And Research | Method for gene splicing by overlap extension using the polymerase chain reaction |
US5360726A (en) | 1989-09-12 | 1994-11-01 | Board Of Trustees Operating Michigan State University | Polypeptides enabling sorting of proteins to vacuoles in plants |
US5030568A (en) * | 1989-09-29 | 1991-07-09 | Minnesota Mining And Manufacturing Company | Bioconversion of naphthalene monomers |
WO1991006570A1 (en) | 1989-10-25 | 1991-05-16 | The University Of Melbourne | HYBRID Fc RECEPTOR MOLECULES |
AU6886991A (en) | 1989-11-08 | 1991-06-13 | United States of America, as represented by the Secretary, U.S. Department of Commerce, The | A method of synthesizing double-stranded dna molecules |
DE4000939A1 (en) * | 1990-01-15 | 1991-07-18 | Brem Gottfried Prof Dr Dr | METHOD FOR OBTAINING ANTIBODIES |
US5427930A (en) | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
KR950013953B1 (en) | 1990-01-26 | 1995-11-18 | 애보트 래보라토리즈 | Method of amplifying target nucleic acids applicable to ligase chain reactions |
CA2079802C (en) | 1990-04-05 | 2001-09-18 | Roberto Crea | Walk-through mutagenesis |
EP0528881A4 (en) | 1990-04-24 | 1993-05-26 | Stratagene | Methods for phenotype creation from multiple gene populations |
US5427908A (en) * | 1990-05-01 | 1995-06-27 | Affymax Technologies N.V. | Recombinant library screening methods |
US5877402A (en) | 1990-05-01 | 1999-03-02 | Rutgers, The State University Of New Jersey | DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein |
US5851813A (en) | 1990-07-12 | 1998-12-22 | President And Fellows Of Harvard College | Primate lentivirus antigenic compositions |
ATE176002T1 (en) * | 1990-07-24 | 1999-02-15 | Hoffmann La Roche | REDUCING NON-SPECIFIC AMPLIFICATION DURING (IN VITRO) NUCLEIC ACID AMPLIFICATION USING MODIFIED NUCLEIC ACID BASES |
US5169764A (en) | 1990-08-08 | 1992-12-08 | Regeneron Pharmaceuticals, Inc. | Multitrophic and multifunctional chimeric neurotrophic factors, and nucleic acids and plasmids encoding the chimeras |
CA2089497A1 (en) * | 1990-08-15 | 1992-02-16 | Lendon Payne | Self-assembling replication defective hybrid virus particles |
WO1992003461A1 (en) | 1990-08-24 | 1992-03-05 | Ixsys, Inc. | Methods of synthesizing oligonucleotides with random codons |
US5264563A (en) | 1990-08-24 | 1993-11-23 | Ixsys Inc. | Process for synthesizing oligonucleotides with random codons |
CA2049879A1 (en) | 1990-08-30 | 1992-03-01 | Keith C. Backman | Controlled initial target-dependent production of templates for ligase chain reaction |
IL99553A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing oligonucleotides linked to expression elements,a kit for the preparation of vectors useful for the expression of a diverse population of random peptides and methods utilizing the same |
US5770434A (en) | 1990-09-28 | 1998-06-23 | Ixsys Incorporated | Soluble peptides having constrained, secondary conformation in solution and method of making same |
US5698426A (en) | 1990-09-28 | 1997-12-16 | Ixsys, Incorporated | Surface expression libraries of heteromeric receptors |
US5871974A (en) | 1990-09-28 | 1999-02-16 | Ixsys Inc. | Surface expression libraries of heteromeric receptors |
US5858725A (en) | 1990-10-10 | 1999-01-12 | Glaxo Wellcome Inc. | Preparation of chimaeric antibodies using the recombinant PCR strategy |
US5187083A (en) | 1990-11-13 | 1993-02-16 | Specialty Laboratories, Inc. | Rapid purification of DNA |
US5234824A (en) | 1990-11-13 | 1993-08-10 | Specialty Laboratories, Inc. | Rapid purification of DNA |
ES2113940T3 (en) | 1990-12-03 | 1998-05-16 | Genentech Inc | ENRICHMENT METHOD FOR PROTEIN VARIANTS WITH ALTERED UNION PROPERTIES. |
US5489523A (en) | 1990-12-03 | 1996-02-06 | Stratagene | Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I |
DE69131017T2 (en) | 1990-12-20 | 1999-07-15 | Ixsys Inc | OPTIMIZATION OF BINDING PROTEINS |
DE4112440C1 (en) | 1991-04-16 | 1992-10-22 | Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De | |
US5795747A (en) | 1991-04-16 | 1998-08-18 | Evotec Biosystems Gmbh | Process for manufacturing new biopolymers |
US5512463A (en) | 1991-04-26 | 1996-04-30 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction library mutagenesis |
US5514568A (en) | 1991-04-26 | 1996-05-07 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction |
DK0519338T3 (en) | 1991-06-20 | 1996-10-28 | Hoffmann La Roche | Improved Methods for Nucleic Acid Amplification |
WO1993000103A1 (en) | 1991-06-21 | 1993-01-07 | The Wistar Institute Of Anatomy And Biology | Chimeric envelope proteins for viral targeting |
DE69232084T2 (en) * | 1991-07-01 | 2002-05-02 | Berlex Lab | Novel methods of mutagenesis and reagents for it |
US6071889A (en) | 1991-07-08 | 2000-06-06 | Neurospheres Holdings Ltd. | In vivo genetic modification of growth factor-responsive neural precursor cells |
US5223408A (en) | 1991-07-11 | 1993-06-29 | Genentech, Inc. | Method for making variant secreted proteins with altered properties |
GB9115364D0 (en) | 1991-07-16 | 1991-08-28 | Wellcome Found | Antibody |
US5279952A (en) | 1991-08-09 | 1994-01-18 | Board Of Regents, The University Of Texas System | PCR-based strategy of constructing chimeric DNA molecules |
AU669489B2 (en) | 1991-09-18 | 1996-06-13 | Affymax Technologies N.V. | Method of synthesizing diverse collections of oligomers |
DK0605522T3 (en) | 1991-09-23 | 2000-01-17 | Medical Res Council | Process for producing humanized antibodies |
US5872215A (en) * | 1991-12-02 | 1999-02-16 | Medical Research Council | Specific binding members, materials and methods |
GB9125979D0 (en) | 1991-12-06 | 1992-02-05 | Wellcome Found | Antibody |
WO1993015208A1 (en) | 1992-01-30 | 1993-08-05 | Genzyme Limited | Chiral synthesis with modified enzymes |
US5773267A (en) * | 1992-02-07 | 1998-06-30 | Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University | D29 shuttle phasmids and uses thereof |
GB9202796D0 (en) | 1992-02-11 | 1992-03-25 | Wellcome Found | Antiviral antibody |
US5843643A (en) | 1992-02-21 | 1998-12-01 | Ratner; Paul L. | Site-specific transfection of eukaryotic cells using polypeptide-linked recombinant nucleic acid |
JPH07507995A (en) | 1992-03-02 | 1995-09-07 | バイオジェン,インコーポレイテッド | thrombin receptor antagonist |
WO1993018175A1 (en) * | 1992-03-10 | 1993-09-16 | Life Technologies, Inc. | Udg-facilitated mutagenesis |
ATE280219T1 (en) | 1992-03-16 | 2004-11-15 | Jacob Nathaniel Wohlstadter | MUTAGENESIS AND SELECTION METHODS IN THE SAME HOST CELLS |
CA2131151A1 (en) | 1992-03-24 | 1994-09-30 | Kevin S. Johnson | Methods for producing members of specific binding pairs |
CA2133220A1 (en) | 1992-03-31 | 1993-10-14 | Stanley R. Bouma | Method of multiplex ligase chain reaction |
US5316935A (en) | 1992-04-06 | 1994-05-31 | California Institute Of Technology | Subtilisin variants suitable for hydrolysis and synthesis in organic media |
US5474896A (en) * | 1992-05-05 | 1995-12-12 | Institut Pasteur | Nucleotide sequence encoding the enzyme I-SceI and the uses thereof |
JPH08503600A (en) | 1992-05-29 | 1996-04-23 | アボツト・ラボラトリーズ | Ligase chain reaction initiated by RNA sequences |
CA2115346A1 (en) | 1992-06-15 | 1993-12-23 | John E. Shively | Chimeric anti-cea antibody |
RU95105971A (en) | 1992-07-10 | 1997-03-20 | Энерджи Биосистемз Корпорейшн (US) | Dna, vectors, microorganism, bacterium, method of fossil fuel desulfurization, probe, proteins |
US6107062A (en) | 1992-07-30 | 2000-08-22 | Inpax, Inc. | Antisense viruses and antisense-ribozyme viruses |
US5837821A (en) | 1992-11-04 | 1998-11-17 | City Of Hope | Antibody construct |
DE4239311C2 (en) | 1992-11-23 | 1996-04-18 | Guehring Joerg Dr | Drills, especially pointed drilling tools with exchangeable cutting inserts |
WO1994012632A1 (en) * | 1992-11-27 | 1994-06-09 | University College London | Improvements in nucleic acid synthesis by pcr |
US5360728A (en) | 1992-12-01 | 1994-11-01 | Woods Hole Oceanographic Institution (W.H.O.I.) | Modified apoaequorin having increased bioluminescent activity |
JP3720353B2 (en) | 1992-12-04 | 2005-11-24 | メディカル リサーチ カウンシル | Multivalent and multispecific binding proteins, their production and use |
US5541311A (en) | 1992-12-07 | 1996-07-30 | Third Wave Technologies, Inc. | Nucleic acid encoding synthesis-deficient thermostable DNA polymerase |
US5571708A (en) | 1993-04-19 | 1996-11-05 | Bristol-Myers Squibb Company | Thrombin-activatable plasminogen activator |
US5501962A (en) | 1993-06-21 | 1996-03-26 | G. D. Searle & Co. | Interleuken-3 (IL-3) human/murine hybrid polypeptides and recombinant production of the same |
IT1264712B1 (en) | 1993-07-13 | 1996-10-04 | Eniricerche Spa | BIOLOGICAL SYNTHESIS METHOD OF PEPTIDES |
US5547859A (en) | 1993-08-02 | 1996-08-20 | Goodman; Myron F. | Chain-terminating nucleotides for DNA sequencing methods |
US5498531A (en) | 1993-09-10 | 1996-03-12 | President And Fellows Of Harvard College | Intron-mediated recombinant techniques and reagents |
US6015686A (en) | 1993-09-15 | 2000-01-18 | Chiron Viagene, Inc. | Eukaryotic layered vector initiation systems |
IL107268A0 (en) | 1993-10-12 | 1994-01-25 | Yeda Res & Dev | Molecules influencing the shedding of the tnf receptor, their preparation and their use |
JPH09507121A (en) | 1993-10-26 | 1997-07-22 | アフィマックス テクノロジーズ ナームロゼ ベノートスハップ | Nucleic acid probe array on biological chip |
US6027877A (en) | 1993-11-04 | 2000-02-22 | Gene Check, Inc. | Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification |
US5556772A (en) | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
DE4343591A1 (en) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Process for the evolutionary design and synthesis of functional polymers based on shape elements and shape codes |
US6406855B1 (en) * | 1994-02-17 | 2002-06-18 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6395547B1 (en) * | 1994-02-17 | 2002-05-28 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US5837458A (en) * | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US6335160B1 (en) * | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US5834252A (en) | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
US6165793A (en) | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5928905A (en) | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
US5521077A (en) | 1994-04-28 | 1996-05-28 | The Leland Stanford Junior University | Method of generating multiple protein variants and populations of protein variants prepared thereby |
US5728821A (en) * | 1994-08-04 | 1998-03-17 | Bristol-Myers Squibb Company | Mutant BR96 antibodies reactive with human carcinomas |
WO1996017056A1 (en) * | 1994-12-02 | 1996-06-06 | Institut Pasteur | Hypermutagenesis |
US5514588A (en) | 1994-12-13 | 1996-05-07 | Exxon Research And Engineering Company | Surfactant-nutrients for bioremediation of hydrocarbon contaminated soils and water |
US5629179A (en) | 1995-03-17 | 1997-05-13 | Novagen, Inc. | Method and kit for making CDNA library |
CA2219136A1 (en) | 1995-04-24 | 1996-10-31 | Chromaxome Corp. | Methods for generating and screening novel metabolic pathways |
US5885813A (en) | 1995-05-31 | 1999-03-23 | Amersham Life Science, Inc. | Thermostable DNA polymerases |
US5667974A (en) | 1995-06-07 | 1997-09-16 | Abbott Laboratories | Method for detecting nucleic acid sequences using competitive amplification |
CA2151183C (en) * | 1995-06-07 | 2005-05-17 | Slawomir Jonasz | Composite compression molded article, composition therefor and process for manufacture thereof, and use |
US6004788A (en) | 1995-07-18 | 1999-12-21 | Diversa Corporation | Enzyme kits and libraries |
US5958672A (en) | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
US6168919B1 (en) | 1996-07-17 | 2001-01-02 | Diversa Corporation | Screening methods for enzymes and enzyme kits |
US6057103A (en) | 1995-07-18 | 2000-05-02 | Diversa Corporation | Screening for novel bioactivities |
US6030779A (en) | 1995-07-18 | 2000-02-29 | Diversa Corporation | Screening for novel bioactivities |
EP1213350A3 (en) | 1995-08-11 | 2002-12-04 | Novozymes A/S | Method for preparing polypeptide variants by in vivo recombination |
US5962258A (en) | 1995-08-23 | 1999-10-05 | Diversa Corporation | Carboxymethyl cellulase fromthermotoga maritima |
US6051409A (en) | 1995-09-25 | 2000-04-18 | Novartis Finance Corporation | Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells |
US5756316A (en) | 1995-11-02 | 1998-05-26 | Genencor International, Inc. | Molecular cloning by multimerization of plasmids |
US20030215798A1 (en) | 1997-06-16 | 2003-11-20 | Diversa Corporation | High throughput fluorescence-based screening for novel enzymes |
US6171820B1 (en) | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
US6352842B1 (en) | 1995-12-07 | 2002-03-05 | Diversa Corporation | Exonucease-mediated gene assembly in directed evolution |
US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
US6358709B1 (en) * | 1995-12-07 | 2002-03-19 | Diversa Corporation | End selection in directed evolution |
US6238884B1 (en) | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
US6361974B1 (en) | 1995-12-07 | 2002-03-26 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
US5871969A (en) * | 1996-02-12 | 1999-02-16 | Human Genome Sciences, Inc. | Nucleic acids encoding human neuronal attachment factor-1 |
US6096548A (en) | 1996-03-25 | 2000-08-01 | Maxygen, Inc. | Method for directing evolution of a virus |
US5783431A (en) | 1996-04-24 | 1998-07-21 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
JP2000512142A (en) * | 1996-06-07 | 2000-09-19 | マサチューセッツ インスティチュート オブ テクノロジー | Programmed continuous mutagenesis |
US5763239A (en) | 1996-06-18 | 1998-06-09 | Diversa Corporation | Production and use of normalized DNA libraries |
US6093873A (en) | 1996-08-19 | 2000-07-25 | Institut National De La Sante Et De La Recherche Medicale | Genetically engineered mice containing alterations in the gene encoding RXR |
US6326204B1 (en) * | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
US6159687A (en) | 1997-03-18 | 2000-12-12 | Novo Nordisk A/S | Methods for generating recombined polynucleotides |
US5948653A (en) | 1997-03-21 | 1999-09-07 | Pati; Sushma | Sequence alterations using homologous recombination |
US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
US6051049A (en) | 1997-05-29 | 2000-04-18 | Exothermic Distribution Corporation | Utilization of strontium aluminate in steelmaking |
US6087341A (en) | 1998-02-12 | 2000-07-11 | The Board Of Trustees Of The Leland Standford Junior University | Introduction of nucleic acid into skin cells by topical application |
US6365408B1 (en) | 1998-06-19 | 2002-04-02 | Maxygen, Inc. | Methods of evolving a polynucleotides by mutagenesis and recombination |
US6376246B1 (en) * | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US6436675B1 (en) * | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
EP1152058A1 (en) * | 2000-05-03 | 2001-11-07 | Institut Curie | Methods and compositions for effecting homologous recombination |
US6355160B1 (en) | 2000-07-21 | 2002-03-12 | Cecil A. Wiseman | Gray-water recycling system |
-
1996
- 1996-12-18 US US08/769,062 patent/US6335160B1/en not_active Expired - Lifetime
-
1997
- 1997-12-17 AT AT01202350T patent/ATE481480T1/en active
- 1997-12-17 ES ES01202350T patent/ES2351673T3/en not_active Expired - Lifetime
- 1997-12-17 IL IL13019097A patent/IL130190A0/en unknown
- 1997-12-17 CA CA2274319A patent/CA2274319C/en not_active Expired - Fee Related
- 1997-12-17 EP EP01202350A patent/EP1149905B1/en not_active Expired - Lifetime
- 1997-12-17 DK DK10075154.4T patent/DK2202308T3/en active
- 1997-12-17 EP EP97953571A patent/EP0946755A4/en not_active Withdrawn
- 1997-12-17 WO PCT/US1997/024239 patent/WO1998027230A1/en not_active Application Discontinuation
- 1997-12-17 KR KR1019997005569A patent/KR20000069591A/en not_active Application Discontinuation
- 1997-12-17 EP EP01202349A patent/EP1149904A1/en not_active Withdrawn
- 1997-12-17 AU AU57292/98A patent/AU732146B2/en not_active Withdrawn - After Issue
- 1997-12-17 DK DK01202350.3T patent/DK1149905T3/en active
- 1997-12-17 CA CA002589337A patent/CA2589337A1/en not_active Abandoned
- 1997-12-17 EP EP10075154A patent/EP2202308B1/en not_active Expired - Lifetime
- 1997-12-17 EP EP02001412A patent/EP1231269A1/en not_active Withdrawn
- 1997-12-17 DE DE69739996T patent/DE69739996D1/en not_active Expired - Lifetime
- 1997-12-17 JP JP52805498A patent/JP5008784B2/en not_active Expired - Fee Related
-
1999
- 1999-06-24 US US09/339,913 patent/US6303344B1/en not_active Expired - Lifetime
- 1999-06-24 US US09/344,002 patent/US6355484B1/en not_active Expired - Lifetime
- 1999-06-24 US US09/339,926 patent/US6653072B1/en not_active Expired - Fee Related
- 1999-06-25 US US09/339,904 patent/US6319713B1/en not_active Expired - Lifetime
-
2000
- 2000-04-27 US US09/559,565 patent/US6455253B1/en not_active Expired - Fee Related
- 2000-04-27 US US09/559,671 patent/US6613514B2/en not_active Expired - Fee Related
- 2000-10-20 US US09/693,350 patent/US6579678B1/en not_active Expired - Fee Related
- 2000-10-20 US US09/693,389 patent/US6586182B1/en not_active Expired - Fee Related
-
2001
- 2001-09-12 US US09/954,692 patent/US6946296B2/en not_active Expired - Fee Related
-
2003
- 2003-08-22 US US10/646,221 patent/US7534564B2/en not_active Expired - Fee Related
- 2003-09-22 US US10/667,772 patent/US20040214277A1/en not_active Abandoned
- 2003-09-22 US US10/667,868 patent/US20050059109A1/en not_active Abandoned
-
2005
- 2005-05-24 US US11/136,115 patent/US20060166225A1/en not_active Abandoned
- 2005-08-05 US US11/198,765 patent/US20060084091A1/en not_active Abandoned
- 2005-11-23 US US11/286,271 patent/US20060223143A1/en not_active Abandoned
-
2006
- 2006-09-06 US US11/515,879 patent/US20070031878A1/en not_active Abandoned
-
2008
- 2008-02-05 US US12/069,011 patent/US7776598B2/en not_active Expired - Fee Related
- 2008-12-01 JP JP2008306897A patent/JP2009089710A/en not_active Withdrawn
-
2009
- 2009-09-11 US US12/557,983 patent/US20110053781A1/en not_active Abandoned
-
2011
- 2011-09-01 US US13/224,145 patent/US20120015820A1/en not_active Abandoned
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2183661A (en) * | 1985-03-30 | 1987-06-10 | Marc Ballivet | Method for obtaining dna, rna, peptides, polypeptides or proteins by means of a dna recombinant technique |
WO1989006694A1 (en) * | 1988-01-15 | 1989-07-27 | Trustees Of The University Of Pennsylvania | Process for selection of proteinaceous substances which mimic growth-inducing molecules |
WO1991000925A1 (en) * | 1989-07-13 | 1991-01-24 | Massachusetts Institute Of Technology | Determination of a mutational spectrum |
US5565332A (en) * | 1991-09-23 | 1996-10-15 | Medical Research Council | Production of chimeric antibodies - a combinatorial approach |
US5525486A (en) * | 1993-01-14 | 1996-06-11 | Tasuku Honjo | Process for constructing cDNA library, and novel polypeptide and DNA coding for the same |
Non-Patent Citations (15)
Title |
---|
BIOTECH. ADV., 1992, Vol. 10, REIKOFSKI et al., "Polymerase Chain Reaction (PCR) Techniques for Site-Directed Mutagenesis", pages 535-547. * |
CURR. OPIN. STRUCT. BIOL., 1995, Vol. 5, O'NEIL et al., "Phage Display: Protein Engineering by Directed Evolution", pages 443-449. * |
GENE, 1988, Vol. 68, MANDECKI et al., "FokI Method of Gene Synthesis (Recombinant DNA; Oligodeoxyribonucleotide; Bridge Mutagenesis; Cloning; Gene Assembly; HIV)", pages 101-107. * |
J. BIOL. CHEM., 06 September 1996, Vol. 271, No. 36, CHONG et al., "Protein Splicing Involving the Saccharomyces Cerevisiae VMA Intein", pages 22159-22168. * |
J. MOL. BIOL., 1996, Vol. 255, GATES et al., "Affinity Selective Isolation of Ligands from Peptide Libraries Through Display on a Lac Repressor 'Headpiece Dimer'", pages 373-386. * |
MOL. BIOL., July-August 1995, Vol. 29, No. 5, Part 1, LEVICHKIN et al., "A New Approach to Construction of Hybrid Genes: Homolog Recombination Method", pages 572-577. * |
NATURE MED., January 1996, Vol. 2, No. 1, CRAMERI et al., "Construction and Evolution of Antibody-Phage Libraries by DNA Shuffling", pages 100-102. * |
NATURE, 19 April 1990, Vol. 344, JONES et al., "DNA Mutagenesis and Recombination", pages 793-794. * |
NUC. ACIDS RES., 1994, Vol. 22, No. 9, BENDIXEN et al., "A Yeast Mating-Selection Scheme for Detection of Protein-Protein Interactions", pages 1778-1779. * |
PCR METH. APPL., 1992, Vol. 2, CADWELL et al., "Randomization of Genes by PCR Mutagenesis", pages 28-33. * |
PROC. NATL. ACAD. SCI. U.S.A., May 1987, Vol. 84, SEED et al., "Molecular Cloning of the CD2 Antigen, the T-Cell Erythrocyte Receptor, by a Rapid Immunoselection Procedure", pages 3365-3369. * |
PROC. NATL. ACAD. SCI. U.S.A., May 1993, Vol. 90, SWEASY et al., "Detection and Characterization of Mammalian DNA Polymerase beta Mutants by Functional Complementation in Escherichia Coli", pages 4626-4630. * |
PROC. NATL. ACAD. SCI. U.S.A., October 1994, Vol. 91, STEMMER W.P.C., "DNA Shuffling by Random Fragmentation and Reassembly: In Vitro Recombination for Molecular Evolution", pages 10747-10751. * |
PROTEIN ENG., 1992, Vol. 5, No. 8, PRODROMOU et al., "PROTOCOL: Recursive PCR: A Novel Technique for Total Gene Synthesis", pages 827-829. * |
SCIENCE, 21 May 1993, Vol. 260, MATTHEWS et al., "Substrate Phage: Selection of Protease Substrates by Monovalent Phage Display", pages 1113-1117. * |
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US7935800B2 (en) | 1996-01-24 | 2011-05-03 | Third Wave Technologies, Inc. | RNA detection assays |
US7807806B2 (en) | 1996-01-24 | 2010-10-05 | Third Wave Technologies, Inc. | RNA detection assays |
US6242211B1 (en) | 1996-04-24 | 2001-06-05 | Terragen Discovery, Inc. | Methods for generating and screening novel metabolic pathways |
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US6423542B1 (en) | 1999-01-19 | 2002-07-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US6319714B1 (en) | 1999-01-19 | 2001-11-20 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
EP1073710A2 (en) * | 1999-02-04 | 2001-02-07 | Diversa Corporation | Non-stochastic generation of genetic vaccines and enzymes |
EP1073710A4 (en) * | 1999-02-04 | 2007-12-19 | Verenium Corp | Non-stochastic generation of genetic vaccines and enzymes |
US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US7384387B1 (en) | 1999-02-11 | 2008-06-10 | Maxygen, Inc. | High throughput mass spectrometry |
US6531316B1 (en) | 1999-03-05 | 2003-03-11 | Maxyag, Inc. | Encryption of traits using split gene sequences and engineered genetic elements |
WO2000053744A3 (en) * | 1999-03-09 | 2001-01-18 | Diversa Corp | End selection in directed evolution |
WO2000053744A2 (en) * | 1999-03-09 | 2000-09-14 | Diversa Corporation | End selection in directed evolution |
WO2000061740A1 (en) * | 1999-04-10 | 2000-10-19 | Maxygen, Inc. | Modified lipid production |
EP1192280A4 (en) * | 1999-06-14 | 2006-04-05 | Diversa Corp | Synthetic ligation reassembly in directed evolution |
AU2009212959B2 (en) * | 1999-06-14 | 2013-08-15 | Bp Corporation North America Inc. | Synthetic ligation reassembly in directed evolution |
EP1192280A1 (en) * | 1999-06-14 | 2002-04-03 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
EP2865754A1 (en) * | 1999-06-14 | 2015-04-29 | BP Corporation North America Inc. | Synthetic ligation reassembly in directed evolution |
US6605449B1 (en) | 1999-06-14 | 2003-08-12 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
USRE45349E1 (en) | 1999-06-14 | 2015-01-20 | Bp Corporation North America Inc. | Synthetic ligation reassembly in directed evolution |
WO2001004287A1 (en) * | 1999-07-07 | 2001-01-18 | Maxygen Aps | A method for preparing modified polypeptides |
US20070037254A1 (en) * | 1999-07-12 | 2007-02-15 | Genentech, Inc. | Expression Vectors and Methods |
US6951719B1 (en) | 1999-08-11 | 2005-10-04 | Proteus S.A. | Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained |
WO2001012791A1 (en) * | 1999-08-12 | 2001-02-22 | Maxygen, Inc. | Dna shuffling of dioxygenase genes for production of industrial chemicals |
US7858340B2 (en) | 1999-08-12 | 2010-12-28 | Pfizer Inc. | Polynucleotides and methods of directing the ratio of B2:B1 avermectins in Streptomyces avermitilis host cells |
US6632673B1 (en) | 1999-08-12 | 2003-10-14 | Pfizer, Inc. | Directing the ratio of B2:B1 avermectins in Streptomyces avermitilis host cells |
US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
WO2001029211A2 (en) * | 1999-10-19 | 2001-04-26 | Enchira Biotechnology Corporation | Method for directed evolution by random chimeragenesis on transient templates |
WO2001029212A1 (en) * | 1999-10-19 | 2001-04-26 | Enchira Biotechnology Corporation | Methods for chimeragenesis of whole genomes or large polynucleotides |
WO2001029211A3 (en) * | 1999-10-19 | 2002-01-24 | Enchira Biotechnology Corp | Method for directed evolution by random chimeragenesis on transient templates |
WO2001032712A3 (en) * | 1999-11-03 | 2002-03-21 | Maxygen Inc | Antibody diversity generation |
WO2001032712A2 (en) * | 1999-11-03 | 2001-05-10 | Maxygen, Inc. | Antibody diversity generation |
US6686515B1 (en) | 1999-11-23 | 2004-02-03 | Maxygen, Inc. | Homologous recombination in plants |
US7115712B1 (en) | 1999-12-02 | 2006-10-03 | Maxygen, Inc. | Cytokine polypeptides |
WO2001064864A2 (en) * | 2000-02-28 | 2001-09-07 | Maxygen, Inc. | Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation |
WO2001064864A3 (en) * | 2000-02-28 | 2002-03-28 | Maxygen Inc | Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation |
US6479262B1 (en) | 2000-05-16 | 2002-11-12 | Hercules, Incorporated | Solid phase enzymatic assembly of polynucleotides |
US6635453B2 (en) | 2000-05-16 | 2003-10-21 | Hercules Incorporated | Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics |
WO2001088173A3 (en) * | 2000-05-16 | 2002-06-06 | Hercules Inc | Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics |
WO2001088173A2 (en) * | 2000-05-16 | 2001-11-22 | Hercules Incorporated | Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics |
US6759226B1 (en) | 2000-05-24 | 2004-07-06 | Third Wave Technologies, Inc. | Enzymes for the detection of specific nucleic acid sequences |
US7094875B2 (en) | 2000-06-23 | 2006-08-22 | Maxygen, Inc. | Co-stimulatory polypeptides |
US7074590B2 (en) | 2000-06-23 | 2006-07-11 | Maxygen, Inc. | Chimeric promoters |
US7612170B2 (en) | 2000-06-23 | 2009-11-03 | Maxygen, Inc. | Co-stimulatory polypeptides |
WO2002004630A3 (en) * | 2000-07-10 | 2002-08-29 | Ambion Inc | Methods for recombinatorial nucleic acid synthesis |
US6994963B1 (en) | 2000-07-10 | 2006-02-07 | Ambion, Inc. | Methods for recombinatorial nucleic acid synthesis |
WO2002004630A2 (en) * | 2000-07-10 | 2002-01-17 | Ambion, Inc. | Methods for recombinatorial nucleic acid synthesis |
US7407786B2 (en) | 2000-07-13 | 2008-08-05 | Codexis, Inc. | Lipase genes |
US6858422B2 (en) | 2000-07-13 | 2005-02-22 | Codexis, Inc. | Lipase genes |
WO2002006469A3 (en) * | 2000-07-18 | 2002-08-15 | Enchira Biotechnology Corp | Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids |
WO2002006469A2 (en) * | 2000-07-18 | 2002-01-24 | Enchira Biotechnology Corporation | Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids |
US7087415B2 (en) | 2000-07-31 | 2006-08-08 | Athena Biotechnologies, Inc. | Methods and compositions for directed gene assembly |
US8999328B2 (en) | 2000-12-08 | 2015-04-07 | Alexion Pharmaceuticals, Inc. | Polypeptides and antibodies derived from chronic lymphocytic Leukemia cells and uses thereof |
US8840885B2 (en) | 2000-12-08 | 2014-09-23 | Alexion Pharmaceuticals, Inc. | Methods for treating chronic lymphocytic leukemia |
US9150661B2 (en) | 2000-12-08 | 2015-10-06 | Alexion Pharmaceuticals, Inc. | Polypeptides and antibodies derived from chronic lymphocytic leukemia cells and uses thereof |
US7704715B2 (en) | 2001-04-16 | 2010-04-27 | California Institute Of Technology | Peroxide-driven cytochrome P450 oxygenase variants |
US7465567B2 (en) | 2001-04-16 | 2008-12-16 | California Institute Of Technology | Peroxide-driven cytochrome P450 oxygenase variants |
AU2002254773B2 (en) * | 2001-05-03 | 2005-12-08 | Rensselaer Polytechnic Institute | Novel methods of directed evolution |
WO2002090536A2 (en) * | 2001-05-10 | 2002-11-14 | Novozymes A/S | A method for producing recombined polynucleotides |
WO2002090536A3 (en) * | 2001-05-10 | 2004-03-04 | Novozymes As | A method for producing recombined polynucleotides |
EP1421203A4 (en) * | 2001-05-17 | 2005-06-01 | Diversa Corp | Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof |
EP1421203A2 (en) * | 2001-05-17 | 2004-05-26 | Diversa Corporation | Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof |
WO2002099080A2 (en) * | 2001-06-05 | 2002-12-12 | Gorilla Genomics, Inc. | Methods for low background cloning of dna using long oligonucleotides |
WO2002099080A3 (en) * | 2001-06-05 | 2003-02-20 | Gorilla Genomics Inc | Methods for low background cloning of dna using long oligonucleotides |
US8367386B2 (en) | 2001-07-20 | 2013-02-05 | California Institute Of Technology | Cytochrome P450 oxygenases |
US8076114B2 (en) | 2001-07-20 | 2011-12-13 | California Institute Of Technology | Cytochrome P450 oxygenases |
US9322001B2 (en) | 2001-07-20 | 2016-04-26 | California Institute Of Technology | Cytochrome P450 oxygenases |
US8722371B2 (en) | 2001-07-20 | 2014-05-13 | California Institute Of Technology | Cytochrome P450 oxygenases |
US7691616B2 (en) | 2001-07-20 | 2010-04-06 | California Institute Of Technology | Cytochrome P450 oxygenases |
US7226768B2 (en) | 2001-07-20 | 2007-06-05 | The California Institute Of Technology | Cytochrome P450 oxygenases |
WO2003010311A3 (en) * | 2001-07-23 | 2003-12-11 | Dsm Ip Assets Bv | Process for preparing variant polynucleotides |
WO2003010311A2 (en) * | 2001-07-23 | 2003-02-06 | Dsm Ip Assets B.V. | Process for preparing variant polynucleotides |
EP2410049A1 (en) | 2002-02-26 | 2012-01-25 | Third Wave Technologies, Inc. | RNA detection enzymes |
EP2315145A1 (en) | 2002-03-01 | 2011-04-27 | Codexis Mayflower Holdings, LLC | Methods, systems, and software for identifying functional biomolecules |
EP2278509A1 (en) | 2002-03-01 | 2011-01-26 | Maxygen Inc. | Methods, systems, and software for identifying functional biomolecules |
EP2390803A1 (en) | 2002-03-01 | 2011-11-30 | Codexis Mayflower Holdings, LLC | Methods, systems, and software for identifying functional biomolecules |
WO2003075129A2 (en) | 2002-03-01 | 2003-09-12 | Maxygen, Inc. | Methods, systems, and software for identifying functional bio-molecules |
WO2003078583A2 (en) | 2002-03-09 | 2003-09-25 | Maxygen, Inc. | Optimization of crossover points for directed evolution |
EP2298871A1 (en) | 2002-04-19 | 2011-03-23 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
EP2275536A1 (en) | 2002-08-06 | 2011-01-19 | Verdia, Inc. | AP1 amine oxidase variants |
EP1558748A4 (en) * | 2002-11-01 | 2008-10-22 | Promega Corp | Cell lysis compositions, methods of use, apparatus, and kit |
EP1558748A2 (en) * | 2002-11-01 | 2005-08-03 | Promega Corporation | Cell lysis compositions, methods of use, apparatus, and kit |
EP3023498A1 (en) | 2003-03-06 | 2016-05-25 | BASF Enzymes LLC | Amylases, nucleic acids encoding them and methods for making and using them |
EP2194133A2 (en) | 2003-03-06 | 2010-06-09 | Verenium Corporation | Amylases, nucleic acids encoding them and methods for making and using them |
EP3508578A1 (en) | 2003-03-06 | 2019-07-10 | BASF Enzymes, LLC | Amylases, nucleic acids encoding them and methods for making and using them |
EP2853593A1 (en) | 2003-03-07 | 2015-04-01 | DSM IP Assets B.V. | Hydrolases, nucleic acids encoding them and mehods for making and using them |
WO2004090099A2 (en) | 2003-04-04 | 2004-10-21 | Diversa Corporation | Pectate lyases, nucleic acids encoding them and methods for making and using them |
EP2341136A1 (en) | 2003-04-04 | 2011-07-06 | Verenium Corporation | Pectate lyases, Nucleic Acids encoding them and methods for making and using them |
EP2535414A1 (en) | 2003-04-29 | 2012-12-19 | Pioneer Hi-Bred International Inc. | Novel glyphosate-n-acetyltransferase (gat) genes |
EP2322629A2 (en) | 2003-04-29 | 2011-05-18 | Pioneer Hi-Bred International Inc. | Novel glyphosate-n-acetyltransferase (GAT) genes |
US8741616B2 (en) | 2003-06-17 | 2014-06-03 | California Institute Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome P450 |
US7863030B2 (en) | 2003-06-17 | 2011-01-04 | The California Institute Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome P450 |
US7524664B2 (en) | 2003-06-17 | 2009-04-28 | California Institute Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome P450 |
US8343744B2 (en) | 2003-06-17 | 2013-01-01 | The California Institute Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome P450 |
US8603949B2 (en) | 2003-06-17 | 2013-12-10 | California Institute Of Technology | Libraries of optimized cytochrome P450 enzymes and the optimized P450 enzymes |
US9145549B2 (en) | 2003-06-17 | 2015-09-29 | The California Institute Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome P450 |
EP2404928A1 (en) | 2003-07-02 | 2012-01-11 | Verenium Corporation | Glucanases, nucleic acids encoding them and methods for making and using them |
EP2404929A1 (en) | 2003-07-02 | 2012-01-11 | Verenium Corporation | Glucanases, nucleic acids encoding them and methods for making and using them |
EP2404931A1 (en) | 2003-07-02 | 2012-01-11 | Verenium Corporation | Glucanases, nucleic acids encoding them and methods for making and using them |
EP2404930A1 (en) | 2003-07-02 | 2012-01-11 | Verenium Corporation | Glucanases, nucleic acids encoding them and methods for making and using them |
EP2409981A1 (en) | 2003-07-02 | 2012-01-25 | Verenium Corporation | Glucanases, nucleic acids encoding them and methods for making and using them |
US7435570B2 (en) | 2003-08-11 | 2008-10-14 | California Institute Of Technology | Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use |
WO2005021714A2 (en) | 2003-08-11 | 2005-03-10 | Diversa Corporation | Laccases, nucleic acids encoding them and methods for making and using them |
US7354734B2 (en) | 2003-08-25 | 2008-04-08 | Funzyme Biotechnologies Sa | Fungal proteins and nucleic acids encoding same |
US7943340B2 (en) | 2003-08-25 | 2011-05-17 | Funzyme Biotechnologies Sa | Fungal dipeptidyl peptidase IV (DPPIV) enzyme for reagent use |
US7468267B2 (en) | 2003-08-25 | 2008-12-23 | Funzyme Biotechnologies Sa | Fungal proteins and nucleic acids encoding same |
WO2005082077A2 (en) | 2004-02-25 | 2005-09-09 | Pioneer Hi-Bred International, Inc. | Novel bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof |
EP2184360A2 (en) | 2004-02-25 | 2010-05-12 | Pioneer Hi-Bred International Inc. | Novel bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof |
EP3190182A1 (en) | 2004-03-08 | 2017-07-12 | DSM IP Assets B.V. | Phospholipases, nucleic acids encoding them and methods for making and using them |
WO2005123929A2 (en) | 2004-06-09 | 2005-12-29 | Pioneer Hi-Bred International, Inc. | Plastid transit peptides |
EP2468853A1 (en) | 2004-06-16 | 2012-06-27 | Verenium Corporation | Composition and methods for enzymatic decolorization of chlorophyll |
WO2006009676A2 (en) | 2004-06-16 | 2006-01-26 | Diversa Corporation | Compositions and methods for enzymatic decolorization of chlorophyll |
EP2886658A1 (en) | 2005-03-10 | 2015-06-24 | BASF Enzymes LLC | Lyase enzymes, nucleic acids encoding them and methods for making and using them |
WO2006101584A2 (en) | 2005-03-15 | 2006-09-28 | Diversa Corporation | Cellulases, nucleic acids encoding them and methods for making and using them |
EP2949756A2 (en) | 2005-03-15 | 2015-12-02 | BP Corporation North America Inc. | Cellulases, nucleic acids encoding them and methods for making and using them |
US10648006B2 (en) | 2005-03-28 | 2020-05-12 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US11214817B2 (en) | 2005-03-28 | 2022-01-04 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US9963720B2 (en) | 2005-03-28 | 2018-05-08 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US9404096B2 (en) | 2005-03-28 | 2016-08-02 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US8715988B2 (en) | 2005-03-28 | 2014-05-06 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US8709415B2 (en) | 2006-01-12 | 2014-04-29 | Alexion Pharmaceuticals, Inc. | Antibodies to OX-2/CD200 and uses thereof |
US9000133B2 (en) | 2006-01-12 | 2015-04-07 | Alexion Pharmaceuticals, Inc. | Antibodies to OX-2/CD200 and uses thereof |
EP2216403A2 (en) | 2006-02-02 | 2010-08-11 | Verenium Corporation | Esterases and related nucleic acids and methods |
EP2444487A1 (en) | 2006-02-10 | 2012-04-25 | Verenium Corporation | Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them |
EP2444489A1 (en) | 2006-02-10 | 2012-04-25 | Verenium Corporation | Cellucloytic enzymes, nucleic acids encoding them and methods for making and using them |
EP2444488A1 (en) | 2006-02-10 | 2012-04-25 | Verenium Corporation | Cellucloytic enzymes, nucleic acids encoding them and methods for making and using them |
EP2444490A1 (en) | 2006-02-10 | 2012-04-25 | Verenium Corporation | Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them |
EP2447363A1 (en) | 2006-02-10 | 2012-05-02 | Verenium Corporation | Cellucloytic enzymes, nucleic acids encoding them and methods for making and using them |
EP2450439A1 (en) | 2006-02-10 | 2012-05-09 | Verenium Corporation | Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them |
EP2420570A1 (en) | 2006-02-10 | 2012-02-22 | Verenium Corporation | Arabinofuranosidase enzymes, nucleic acids encoding them and methods for making and using them |
EP2415864A1 (en) | 2006-02-10 | 2012-02-08 | Verenium Corporation | Oligomerase-2 (or beta-xylosidase) enzymes, nucleic acids encoding them and methods for making and using them |
EP2548956A1 (en) | 2006-02-14 | 2013-01-23 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
WO2007095398A2 (en) | 2006-02-14 | 2007-08-23 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
EP2548954A1 (en) | 2006-02-14 | 2013-01-23 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
EP2548955A1 (en) | 2006-02-14 | 2013-01-23 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
EP2385108A1 (en) | 2006-03-07 | 2011-11-09 | Verenium Corporation | Aldolases, nucleic acids encoding them and methods for making and using them |
EP2316962A1 (en) | 2006-03-07 | 2011-05-04 | Cargill, Incorporated | Aldolases, nucleic acids encoding them and methods for making and using them |
EP2322643A1 (en) | 2006-03-07 | 2011-05-18 | Cargill, Incorporated | Aldolases, nucleic acids encoding them and methods for making and using them |
EP3153580A2 (en) | 2006-03-07 | 2017-04-12 | BASF Enzymes LLC | Aldolases, nucleic acids encoding them and methods for making and using them |
EP2112227A1 (en) | 2006-03-07 | 2009-10-28 | Cargill, Incorporated | Aldolases, nucleic acids encoding them and methods for making and using them |
EP2388316A2 (en) | 2006-03-07 | 2011-11-23 | Verenium Corporation | Aldolases, nucleic acids encoding them and methods for making and using them |
EP2639301A2 (en) | 2006-06-30 | 2013-09-18 | Bristol-Myers Squibb Company | Polynucleotides encoding novel PCSK9 variants |
WO2008105797A2 (en) | 2006-06-30 | 2008-09-04 | Bristol-Myers Squibb Company | Polynucleotides encoding novel pcsk9 variants |
EP2671946A1 (en) | 2006-06-30 | 2013-12-11 | Bristol-Myers Squibb Company | Polynucleotides encoding novel PCSK9 variants |
US8026085B2 (en) | 2006-08-04 | 2011-09-27 | California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
US8252559B2 (en) | 2006-08-04 | 2012-08-28 | The California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
EP2444413A1 (en) | 2006-08-04 | 2012-04-25 | Verenium Corporation | Methods for oil or gas well drilling, washing and/or fracturing |
EP2617815A2 (en) | 2006-09-21 | 2013-07-24 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
EP2617821A2 (en) | 2006-09-21 | 2013-07-24 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
EP2617729A2 (en) | 2006-09-21 | 2013-07-24 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
WO2008036863A2 (en) | 2006-09-21 | 2008-03-27 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
EP2620495A2 (en) | 2006-09-21 | 2013-07-31 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
EP2617823A2 (en) | 2006-09-21 | 2013-07-24 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
EP2617728A2 (en) | 2006-09-21 | 2013-07-24 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
EP2617820A2 (en) | 2006-09-21 | 2013-07-24 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
EP2617816A2 (en) | 2006-09-21 | 2013-07-24 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
EP2397486A1 (en) | 2006-09-21 | 2011-12-21 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
EP2617819A2 (en) | 2006-09-21 | 2013-07-24 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
EP2617817A2 (en) | 2006-09-21 | 2013-07-24 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
EP2479267A1 (en) | 2006-12-21 | 2012-07-25 | Verenium Corporation | Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them |
EP2479266A1 (en) | 2006-12-21 | 2012-07-25 | Verenium Corporation | Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them |
EP3540053A1 (en) | 2006-12-21 | 2019-09-18 | BASF Enzymes, LLC | Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them |
WO2008080093A2 (en) | 2006-12-21 | 2008-07-03 | Verenium Corporation | Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them |
EP3101128A1 (en) | 2006-12-21 | 2016-12-07 | BASF Enzymes LLC | Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them |
DE102007003143A1 (en) | 2007-01-16 | 2008-07-17 | Henkel Kgaa | New alkaline protease from Bacillus gibsonii and detergents and cleaners containing this novel alkaline protease |
WO2008095033A2 (en) | 2007-01-30 | 2008-08-07 | Verenium Corporation | Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them |
EP2559702A1 (en) | 2007-02-08 | 2013-02-20 | Domantis Limited | Antibody single variable domains against serum albumin |
EP2559704A1 (en) | 2007-02-08 | 2013-02-20 | Domantis Limited | Antibody single variable domains against serum albumin |
EP2559703A1 (en) | 2007-02-08 | 2013-02-20 | Domantis Limited | Antibody single variable domains against serum albumin |
WO2008103248A1 (en) | 2007-02-08 | 2008-08-28 | Codexis, Inc. | Ketoreductases and uses thereof |
WO2008098198A2 (en) | 2007-02-08 | 2008-08-14 | The California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US8986684B2 (en) | 2007-07-25 | 2015-03-24 | Alexion Pharmaceuticals, Inc. | Methods and compositions for treating autoimmune disease |
WO2009029554A2 (en) | 2007-08-24 | 2009-03-05 | Codexis, Inc. | Improved ketoreductase polypeptides for the stereoselective production of (r)-3-hydroxythiolane |
WO2009045627A2 (en) | 2007-10-03 | 2009-04-09 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
EP2708602A2 (en) | 2007-10-03 | 2014-03-19 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
DE102007051092A1 (en) | 2007-10-24 | 2009-04-30 | Henkel Ag & Co. Kgaa | Subtilisin from Becillus pumilus and detergents and cleaners containing this new subtilisin |
EP2612868A1 (en) | 2007-11-01 | 2013-07-10 | Perseid Therapeutics LLC | Immunosuppressive polypeptides and nucleic acids |
EP2385065A1 (en) | 2007-11-01 | 2011-11-09 | Perseid Therapeutics LLC | Immunosuppressive polypeptides and nucleic acids |
EP2612867A1 (en) | 2007-11-01 | 2013-07-10 | Perseid Therapeutics LLC | Immunosuppressive polypeptides and nucleic acids |
WO2009058564A2 (en) | 2007-11-01 | 2009-05-07 | Maxygen, Inc. | Immunosuppressive polypeptides and nucleic acids |
EP2865750A2 (en) | 2008-01-03 | 2015-04-29 | BASF Enzymes LLC | Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them |
WO2009088949A1 (en) | 2008-01-03 | 2009-07-16 | Verenium Corporation | Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them |
EP2706122A2 (en) | 2008-01-03 | 2014-03-12 | Verenium Corporation | Isomerases, nucleic acids encoding them and methods for making and using them |
US8768871B2 (en) | 2008-02-12 | 2014-07-01 | Codexis, Inc. | Method of generating an optimized, diverse population of variants |
US10155943B2 (en) | 2008-02-12 | 2018-12-18 | Codexis, Inc. | Method of generating an optimized, diverse population of variants |
US8504498B2 (en) | 2008-02-12 | 2013-08-06 | Codexis, Inc. | Method of generating an optimized, diverse population of variants |
EP3115459A2 (en) | 2008-05-23 | 2017-01-11 | E. I. du Pont de Nemours and Company | Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
US8383346B2 (en) | 2008-06-13 | 2013-02-26 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
EP3023494A1 (en) | 2008-06-13 | 2016-05-25 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
WO2009152336A1 (en) | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
US8574876B2 (en) | 2008-06-24 | 2013-11-05 | Codexis, Inc. | Biocatalytic processes for the preparation of stereomerically pure fused bicyclic proline compounds |
US8859784B2 (en) | 2008-06-24 | 2014-10-14 | Codexis, Inc. | Stereomerically pure fused bicyclic proline compounds useful for preparing hepatitis C protease inhibitors |
US8178333B2 (en) | 2008-06-24 | 2012-05-15 | Codexis, Inc. | Biocatalytic processes for the preparation of substantially stereomerically pure fused bicyclic proline compounds |
EP2481737A1 (en) | 2008-06-24 | 2012-08-01 | Merck Sharp & Dohme Corp. | Biocatalytic processes for the preparation of substantially stereomerically pure fused bicyclic proline compounds |
US8455230B2 (en) | 2008-08-27 | 2013-06-04 | Codexis, Inc. | Polynucleotides encoding engineered ketoreductase polypeptides |
US8426178B2 (en) | 2008-08-27 | 2013-04-23 | Codexis, Inc. | Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine |
WO2010025238A2 (en) | 2008-08-27 | 2010-03-04 | Codexis, Inc. | Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine |
US8288131B2 (en) | 2008-08-27 | 2012-10-16 | Codexis, Inc. | Ketoreductase polypeptides and uses thereof |
US8673607B2 (en) | 2008-08-27 | 2014-03-18 | Codexis, Inc. | Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine |
US8288141B2 (en) | 2008-08-27 | 2012-10-16 | Codexis, Inc. | Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine |
WO2010025395A2 (en) | 2008-08-29 | 2010-03-04 | Verenium Corporation | Hydrolases, nucleic acids encoding them and methods for making and using them |
US8273554B2 (en) | 2008-08-29 | 2012-09-25 | Codexis, Inc. | Ketoreductase polypeptides for the stereoselective production of (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one |
US8956840B2 (en) | 2008-08-29 | 2015-02-17 | Codexis, Inc. | Engineered ketoreductase polypeptides |
US10246687B2 (en) | 2008-08-29 | 2019-04-02 | Codexis, Inc. | Ketoreductase polypeptides |
US8415126B2 (en) | 2008-08-29 | 2013-04-09 | Codexis, Inc. | Polynucleotides encoding recombinant ketoreductase polypeptides |
US9139820B2 (en) | 2008-08-29 | 2015-09-22 | Codexis, Inc. | Ketoreductase polypeptides |
US10047348B2 (en) | 2008-08-29 | 2018-08-14 | Codexis, Inc. | Ketoreductase polypeptides |
US10988739B2 (en) | 2008-08-29 | 2021-04-27 | Codexis, Inc. | Ketoreductase polypeptides |
US10544401B2 (en) | 2008-08-29 | 2020-01-28 | Codexis, Inc. | Ketoreductase polypeptides |
US9422530B2 (en) | 2008-08-29 | 2016-08-23 | Codexis, Inc. | Ketoreductase polypeptides |
US9796964B2 (en) | 2008-08-29 | 2017-10-24 | Codexis, Inc. | Ketoreductase polypeptides |
EP3460061A1 (en) | 2008-09-26 | 2019-03-27 | Tocagen Inc. | Recombinant replication competent murine leukemia gamma-retrovirus (mlv) for the expression of heat-stabilized cytosine deaminase |
WO2010081053A2 (en) | 2009-01-08 | 2010-07-15 | Codexis, Inc. | Transaminase polypeptides |
EP3354727A1 (en) | 2009-01-08 | 2018-08-01 | Codexis, Inc. | Transaminase polypeptides |
US8715996B2 (en) | 2009-02-26 | 2014-05-06 | Codexis, Inc. | Beta-glucosidase variant enzymes and related polynucleotides |
WO2010107644A2 (en) | 2009-03-17 | 2010-09-23 | Codexis, Inc. | Variant endoglucanases and related polynucleotides |
US9464278B2 (en) | 2009-03-17 | 2016-10-11 | Codexis, Inc. | Variant endoglucanases and related polynucleotides |
US9005946B2 (en) | 2009-03-17 | 2015-04-14 | Codexis, Inc. | Variant endoglucanases and related polynucleotides |
WO2010120557A1 (en) | 2009-03-31 | 2010-10-21 | Codexis, Inc. | Improved endoglucanases |
EP2698374A1 (en) | 2009-05-21 | 2014-02-19 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
WO2010135588A2 (en) | 2009-05-21 | 2010-11-25 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
EP3026113A2 (en) | 2009-06-11 | 2016-06-01 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
WO2010144103A1 (en) | 2009-06-11 | 2010-12-16 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
WO2010148128A1 (en) | 2009-06-16 | 2010-12-23 | Codexis, Inc. | Beta-glucosidase variant enzymes and related polynucleotides |
US9404092B2 (en) | 2009-06-22 | 2016-08-02 | Codexis, Inc. | Ketoreductase-mediated stereoselective route to alpha chloroalcohols |
US9029112B2 (en) | 2009-06-22 | 2015-05-12 | Codexis, Inc. | Ketoreductase-mediated stereoselective route to alpha chloroalcohols |
EP3409765A2 (en) | 2009-06-22 | 2018-12-05 | Codexis, Inc. | Ketoreductase-mediated stereoselective route to alpha chloroalcohols |
US8796002B2 (en) | 2009-06-22 | 2014-08-05 | Codexis, Inc. | Polypeptides for a ketoreductase-mediated stereoselective route to alpha chloroalcohols |
WO2011005527A2 (en) | 2009-06-22 | 2011-01-13 | Codexis, Inc. | Ketoreductase-mediated stereoselective route to alpha chloroalcohols |
US9296992B2 (en) | 2009-06-22 | 2016-03-29 | Codexis, Inc. | Ketoreductase-mediated stereoselective route to alpha chloroalcohols |
US8614081B2 (en) | 2009-07-23 | 2013-12-24 | Codexis, Inc. | Nitrilase biocatalysts |
US10358631B2 (en) | 2009-08-19 | 2019-07-23 | Codexis, Inc. | Ketoreductase polypeptides for the preparation of phenylephrine |
US9102959B2 (en) | 2009-08-19 | 2015-08-11 | Codexis, Inc. | Ketoreductase polypeptides for the preparation of phenylephrine |
US9834758B2 (en) | 2009-08-19 | 2017-12-05 | Codexis, Inc. | Ketoreductase polypeptides for the preparation of phenylephrine |
US10590396B2 (en) | 2009-08-19 | 2020-03-17 | Codexis, Inc. | Ketoreductase polypeptides for the preparation of phenylephrine |
US10870835B2 (en) | 2009-08-19 | 2020-12-22 | Codexis, Inc. | Ketoreductase polypeptides for the preparation of phenylephrine |
WO2011022548A2 (en) | 2009-08-19 | 2011-02-24 | Codexis, Inc. | Ketoreductase polypeptides for the preparation of phenylephrine |
US11345898B2 (en) | 2009-08-19 | 2022-05-31 | Codexis, Inc. | Ketoreductase polypeptides for the preparation of phenylephrine |
WO2011022597A1 (en) | 2009-08-20 | 2011-02-24 | Pioneer Hi-Bred International, Inc. | Functional expression of shuffled yeast nitrate transporter (ynti) in maize to improve nitrate uptake under low nitrate environment |
US8785170B2 (en) | 2009-09-04 | 2014-07-22 | Codexis, Inc. | Variant CBH2 cellulases and related polynucleotides |
US9102927B2 (en) | 2009-09-04 | 2015-08-11 | Codexis, Inc. | Variant CBH2 cellulases and related polynucleotides |
WO2011028708A1 (en) | 2009-09-04 | 2011-03-10 | Codexis, Inc. | Variant cbh2 cellulases and related polynucleotides |
EP3301208A1 (en) | 2009-09-18 | 2018-04-04 | Codexis, Inc. | Reduced codon mutagenesis |
US9045712B2 (en) | 2009-10-16 | 2015-06-02 | Bunge Global Innovation, Llc | Oil degumming methods |
WO2011046815A1 (en) | 2009-10-16 | 2011-04-21 | Bunge Oils, Inc. | Oil degumming methods |
US9512382B2 (en) | 2009-10-16 | 2016-12-06 | Bunge Global Innovation, Llc | Oil degumming methods |
WO2011046812A1 (en) | 2009-10-16 | 2011-04-21 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
EP2706119A1 (en) | 2009-11-25 | 2014-03-12 | Codexis, Inc. | Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass |
US8993277B2 (en) | 2009-11-25 | 2015-03-31 | Codexis, Inc. | Polynucleotides encoding beta-glucosidase variants |
US8652813B2 (en) | 2009-11-25 | 2014-02-18 | Codexis, Inc. | Recombinant Thermoascus aurantiacus β-glucosidase variants |
WO2011066187A1 (en) | 2009-11-25 | 2011-06-03 | Codexis, Inc. | Recombinant thermoascus aurantiacus beta-glucosidase variants for production of fermentable sugars from cellulosic biomass |
WO2011071982A2 (en) | 2009-12-08 | 2011-06-16 | Codexis, Inc. | Synthesis of prazole compounds |
WO2011082304A1 (en) | 2009-12-31 | 2011-07-07 | Pioneer Hi-Bred International, Inc. | Engineering plant resistance to diseases caused by pathogens |
US10604781B2 (en) | 2010-02-10 | 2020-03-31 | Codexis, Inc. | Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system |
US11193157B2 (en) | 2010-02-10 | 2021-12-07 | Codexis, Inc. | Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system |
US9714439B2 (en) | 2010-02-10 | 2017-07-25 | Codexis, Inc. | Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system |
US9394551B2 (en) | 2010-02-10 | 2016-07-19 | Codexis, Inc. | Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system |
US10196667B2 (en) | 2010-02-10 | 2019-02-05 | Codexis, Inc. | Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system |
US9080192B2 (en) | 2010-02-10 | 2015-07-14 | Codexis, Inc. | Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system |
US8715962B2 (en) | 2010-03-31 | 2014-05-06 | Codexis, Inc. | Production of geranyl diphosphate |
US9644189B2 (en) | 2010-05-04 | 2017-05-09 | Codexis, Inc. | Biocatalysts for ezetimibe synthesis |
US10995320B2 (en) | 2010-05-04 | 2021-05-04 | Codexis, Inc. | Biocatalysts for Ezetimibe synthesis |
US10053673B2 (en) | 2010-05-04 | 2018-08-21 | Codexis, Inc. | Biocatalysts for Ezetimibe synthesis |
US9040262B2 (en) | 2010-05-04 | 2015-05-26 | Codexis, Inc. | Biocatalysts for ezetimibe synthesis |
US9388391B2 (en) | 2010-05-04 | 2016-07-12 | Codexis, Inc. | Biocatalysts for Ezetimibe synthesis |
US10544400B2 (en) | 2010-05-04 | 2020-01-28 | Codexis, Inc. | Biocatalysts for ezetimibe synthesis |
WO2011140219A1 (en) | 2010-05-04 | 2011-11-10 | Codexis, Inc. | Biocatalysts for ezetimibe synthesis |
WO2011143274A1 (en) | 2010-05-10 | 2011-11-17 | Perseid Therapeutics | Polypeptide inhibitors of vla4 |
US9752171B2 (en) | 2010-05-28 | 2017-09-05 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
WO2011159910A2 (en) | 2010-06-17 | 2011-12-22 | Codexis, Inc. | Biocatalysts and methods for the synthesis of (s)-3-(1-aminoethyl)-phenol |
WO2012006114A2 (en) | 2010-06-28 | 2012-01-12 | Codexis, Inc. | Fatty alcohol forming acyl reductases (fars) and methods of use thereof |
WO2012003299A2 (en) | 2010-06-30 | 2012-01-05 | Codexis, Inc. | Highly stable beta-class carbonic anhydrases useful in carbon capture systems |
WO2012021785A1 (en) | 2010-08-13 | 2012-02-16 | Pioneer Hi-Bred International, Inc. | Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (hppd) activity |
US9006460B2 (en) | 2010-08-16 | 2015-04-14 | Cardiome International Ag | Process for preparing aminocyclohexyl ether compounds |
WO2012024698A1 (en) | 2010-08-20 | 2012-02-23 | Codexis, Inc. | Use of glycoside hydrolase 61 family proteins in processing of cellulose |
US9322027B2 (en) | 2010-08-20 | 2016-04-26 | Shell Oil Company | Expression constructs comprising fungal promoters |
US9493802B2 (en) | 2010-08-20 | 2016-11-15 | Codexis, Inc. | Use of glycohydrolase 61 protein variants with improved thermostability for processing cellulose |
US8877474B2 (en) | 2010-08-20 | 2014-11-04 | Codexis, Inc. | GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity |
WO2012078800A2 (en) | 2010-12-08 | 2012-06-14 | Codexis, Inc. | Biocatalysts and methods for the synthesis of armodafinil |
US9169468B2 (en) | 2011-03-30 | 2015-10-27 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
WO2012142302A2 (en) | 2011-04-13 | 2012-10-18 | Codexis, Inc. | Biocatalytic process for preparing eslicarbazepine and analogs thereof |
US9102963B2 (en) | 2011-04-13 | 2015-08-11 | Codexis, Inc. | Biocatalytic process for preparing eslicarbazepine and analogs thereof |
US9963683B2 (en) | 2011-04-13 | 2018-05-08 | Codexis, Inc. | Biocatalytic process for preparing eslicarbazepine and analogs thereof |
US9605290B2 (en) | 2011-04-13 | 2017-03-28 | Codexis, Inc. | Biocatalytic process for preparing eslicarbazepine and analogs thereof |
US9365878B2 (en) | 2011-04-13 | 2016-06-14 | Codexis, Inc. | Biocatalytic process for preparing eslicarbazepine and analogs thereof |
US9045745B2 (en) | 2011-06-30 | 2015-06-02 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
US8778652B2 (en) | 2011-06-30 | 2014-07-15 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
US9322007B2 (en) | 2011-07-22 | 2016-04-26 | The California Institute Of Technology | Stable fungal Cel6 enzyme variants |
WO2013028701A1 (en) | 2011-08-22 | 2013-02-28 | Codexis, Inc. | Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity |
US8951758B2 (en) | 2011-08-22 | 2015-02-10 | Codexis, Inc. | GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity |
US9624478B2 (en) | 2011-09-08 | 2017-04-18 | Codexis, Inc. | Biocatalysts and methods for the synthesis of substituted lactams |
US10858635B2 (en) | 2011-09-08 | 2020-12-08 | Codexis, Inc. | Biocatalysts and methods for the synthesis of substituted lactams |
US9109209B2 (en) | 2011-09-08 | 2015-08-18 | Codexis, Inc. | Biocatalysts and methods for the synthesis of substituted lactams |
US11597918B2 (en) | 2011-09-08 | 2023-03-07 | Codexis, Inc. | Biocatalysts and methods for the synthesis of substituted lactams |
WO2013036861A1 (en) | 2011-09-08 | 2013-03-14 | Codexis, Inc | Biocatalysts and methods for the synthesis of substituted lactams |
US10633640B2 (en) | 2011-09-08 | 2020-04-28 | Codexis, Inc. | Biocatalysts and methods for the synthesis of substituted lactams |
US9434919B2 (en) | 2011-09-30 | 2016-09-06 | Codexis, Inc. | Fungal proteases |
US9422531B2 (en) | 2011-11-18 | 2016-08-23 | Codexis, Inc. | Biocatalysts for the preparation of hydroxy substituted carbamates |
US10253303B2 (en) | 2011-11-18 | 2019-04-09 | Codexis, Inc. | Biocatalysts for the preparation of hydroxy substituted carbamates |
US11713451B2 (en) | 2011-11-18 | 2023-08-01 | Codexis, Inc. | Biocatalysts for the preparation of hydroxy substituted carbamates |
US10851351B2 (en) | 2011-11-18 | 2020-12-01 | Codexis, Inc. | Biocatalysts for the preparation of hydroxy substituted carbamates |
US9803178B2 (en) | 2011-11-18 | 2017-10-31 | Codexis, Inc. | Biocatalysts for the preparation of hydroxy substituted carbamates |
US9139819B2 (en) | 2011-11-18 | 2015-09-22 | Codexis, Inc. | Biocatalysts for the preparation of hydroxy substituted carbamates |
WO2013074650A1 (en) | 2011-11-18 | 2013-05-23 | Codexis, Inc. | Biocatalysts for the preparation of hydroxy substituted carbamates |
US10100287B2 (en) | 2011-11-18 | 2018-10-16 | Codexis, Inc. | Biocatalysts for the preparation of hydroxy substituted carbamates |
US9611462B2 (en) | 2011-12-20 | 2017-04-04 | Codexis, Inc. | Endoglucanase 1B (EG1B) variants |
US10544402B2 (en) | 2012-03-23 | 2020-01-28 | Codexis Inc. | Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs |
US9708588B2 (en) | 2012-03-23 | 2017-07-18 | Codexis, Inc. | Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs |
US9139821B2 (en) | 2012-03-23 | 2015-09-22 | Codexis, Inc. | Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs |
US9388395B2 (en) | 2012-03-23 | 2016-07-12 | Codexis, Inc. | Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs |
WO2013142770A1 (en) | 2012-03-23 | 2013-09-26 | Codexis, Inc. | Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs |
US11098291B2 (en) | 2012-03-23 | 2021-08-24 | Codexis, Inc. | Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs |
WO2013166113A1 (en) | 2012-05-04 | 2013-11-07 | E. I. Du Pont De Nemours And Company | Compositions and methods comprising sequences having meganuclease activity |
US10370688B2 (en) | 2012-05-08 | 2019-08-06 | Codexis, Inc. | Methods for hydroxylyation of chemical compounds |
US9790527B2 (en) | 2012-05-08 | 2017-10-17 | Codexis, Inc. | Engineered proline hydroxylase polypeptides |
US11549132B2 (en) | 2012-05-08 | 2023-01-10 | Codexis, Inc. | Biocatalysts and methods for hydroxylation of chemical compounds |
WO2013169725A2 (en) | 2012-05-08 | 2013-11-14 | Codexis, Inc. | Biocatalysts and methods for hydroxylation of chemical compounds |
US10995349B2 (en) | 2012-05-08 | 2021-05-04 | Codexis, Inc. | Engineered proline hydroxylase polypeptides and methods |
US10731189B2 (en) | 2012-05-08 | 2020-08-04 | Codexis, Inc. | Engineered proline hydroxylase polynucleotides and methods |
WO2013170050A1 (en) | 2012-05-11 | 2013-11-14 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
US9476077B2 (en) | 2012-06-11 | 2016-10-25 | Codexis, Inc. | Fungal beta-xylosidase variants |
US8980578B2 (en) | 2012-06-11 | 2015-03-17 | Codexis, Inc. | Fungal beta-xylosidase variants |
WO2013188305A2 (en) | 2012-06-11 | 2013-12-19 | Codexis, Inc. | Fungal beta-xylosidase variants |
US8956844B2 (en) | 2012-06-11 | 2015-02-17 | Codexis, Inc. | Fungal xylanases and xylosidases |
EP3450549A1 (en) | 2012-10-29 | 2019-03-06 | University Of Rochester | Artemisinin derivatives, methods for their preparation and their use as antimalarial agents |
US9611515B2 (en) | 2012-11-20 | 2017-04-04 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
EP3406594A1 (en) | 2012-12-07 | 2018-11-28 | Merck Sharp & Dohme Corp. | Biocatalytic transamination process |
EP2921560A4 (en) * | 2012-12-11 | 2016-05-04 | Celemics Inc | Method for synthesizing gene library using codon combination and mutagenesis |
CN104955961A (en) * | 2012-12-11 | 2015-09-30 | 塞勒密斯株式会社 | Method for synthesizing gene library using codon combination and mutagenesis |
US10036007B2 (en) | 2012-12-11 | 2018-07-31 | Celemics, Inc. | Method of synthesis of gene library using codon randomization and mutagenesis |
US11732248B2 (en) | 2012-12-21 | 2023-08-22 | Codexis, Inc. | Engineered biocatalysts and methods for synthesizing chiral amines |
US9902943B2 (en) | 2012-12-21 | 2018-02-27 | Codexis, Inc. | Engineered biocatalysts and methods for synthesizing chiral amines |
US11034939B2 (en) | 2012-12-21 | 2021-06-15 | Codexis, Inc. | Engineered biocatalysts and methods for synthesizing chiral amines |
WO2014099730A1 (en) | 2012-12-21 | 2014-06-26 | Codexis, Inc. | Engineered biocatalysts and methods for synthesizing chiral amines |
US10526587B2 (en) | 2012-12-21 | 2020-01-07 | Codexis, Inc. | Engineered biocatalysts and methods for synthesizing chiral amines |
US10435674B2 (en) | 2012-12-21 | 2019-10-08 | Codexis, Inc. | Engineered biocatalysts and methods for synthesizing chiral amines |
WO2014113521A1 (en) | 2013-01-18 | 2014-07-24 | Codexis, Inc. | Engineered biocatalysts useful for carbapenem synthesis |
US9663771B2 (en) | 2013-01-18 | 2017-05-30 | Codexis, Inc. | Engineered biocatalysts useful for carbapenem synthesis |
US9540622B2 (en) | 2013-01-18 | 2017-01-10 | Codexis, Inc. | Engineered biocatalysts useful for carbapenem synthesis |
WO2014120821A1 (en) | 2013-01-31 | 2014-08-07 | Codexis, Inc. | Methods, systems, and software for identifying bio-molecules using models of multiplicative form |
WO2014120819A1 (en) | 2013-01-31 | 2014-08-07 | Codexis, Inc. | Methods, systems, and software for identifying bio-molecules with interacting components |
US11098292B2 (en) | 2013-02-28 | 2021-08-24 | Codexis, Inc. | Engineered transaminase polypeptides for industrial biocatalysis |
WO2014133960A1 (en) | 2013-02-28 | 2014-09-04 | Codexis, Inc. | Engineered transaminase polypeptides for industrial biocatalysis |
US9963685B2 (en) | 2013-02-28 | 2018-05-08 | Codexis, Inc. | Engineered transaminase polypeptides for industrial biocatalysis |
US10604744B2 (en) | 2013-02-28 | 2020-03-31 | Codexis, Inc. | Engineered transaminase polypeptides for industrial biocatalysis |
US9617573B2 (en) | 2013-02-28 | 2017-04-11 | Codexis, Inc. | Engineered transaminase polypeptides for industrial biocatalysis |
WO2014153234A1 (en) | 2013-03-14 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Compositions having dicamba decarboxylase activity and methods of use |
WO2014153242A1 (en) | 2013-03-14 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Compositions having dicamba decarboxylase activity and methods of use |
WO2014150914A2 (en) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Phi-4 polypeptides and methods for their use |
US10494623B2 (en) | 2013-04-18 | 2019-12-03 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US10640759B2 (en) | 2013-04-18 | 2020-05-05 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US10160963B2 (en) | 2013-04-18 | 2018-12-25 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US10487319B2 (en) | 2013-04-18 | 2019-11-26 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US11136571B2 (en) | 2013-04-18 | 2021-10-05 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US10717974B2 (en) | 2013-04-18 | 2020-07-21 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US11130947B2 (en) | 2013-04-18 | 2021-09-28 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US9611468B2 (en) | 2013-04-18 | 2017-04-04 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US11913045B2 (en) | 2013-04-18 | 2024-02-27 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
WO2014172541A2 (en) | 2013-04-18 | 2014-10-23 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US10870843B2 (en) | 2013-04-18 | 2020-12-22 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US10975367B2 (en) | 2013-04-18 | 2021-04-13 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US10294468B2 (en) | 2013-04-18 | 2019-05-21 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US10941391B2 (en) | 2013-04-18 | 2021-03-09 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
EP3971288A1 (en) | 2013-04-18 | 2022-03-23 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US10358639B2 (en) | 2013-04-18 | 2019-07-23 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
EP3521423A1 (en) | 2013-04-18 | 2019-08-07 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US10144924B2 (en) | 2013-04-18 | 2018-12-04 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US11401515B2 (en) | 2013-04-18 | 2022-08-02 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
WO2015023846A2 (en) | 2013-08-16 | 2015-02-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2015038734A2 (en) | 2013-09-13 | 2015-03-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3692786A1 (en) | 2013-09-13 | 2020-08-12 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP4159028A1 (en) | 2013-09-13 | 2023-04-05 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3418929A1 (en) | 2013-09-27 | 2018-12-26 | Codexis, Inc. | Automated screening of enzyme variants |
US10407668B2 (en) | 2013-11-13 | 2019-09-10 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
US9822346B2 (en) | 2013-11-13 | 2017-11-21 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
US11060072B2 (en) | 2013-11-13 | 2021-07-13 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
WO2015073555A1 (en) | 2013-11-13 | 2015-05-21 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
EP4122945A1 (en) | 2013-12-23 | 2023-01-25 | University of Rochester | Methods and compositions for ribosomal synthesis of macrocyclic peptides |
WO2015120270A1 (en) | 2014-02-07 | 2015-08-13 | Pioneer Hi Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3705489A1 (en) | 2014-02-07 | 2020-09-09 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2015120276A1 (en) | 2014-02-07 | 2015-08-13 | Pioneer Hi Bred International Inc | Insecticidal proteins and methods for their use |
US9605252B2 (en) | 2014-04-16 | 2017-03-28 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
WO2015161019A1 (en) | 2014-04-16 | 2015-10-22 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
US10287567B2 (en) | 2014-04-16 | 2019-05-14 | Codexis, Inc. | Polynucleotides encoding engineered tyrosine ammonia lyase variants |
US10533168B2 (en) | 2014-04-16 | 2020-01-14 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
EP3928787A1 (en) | 2014-04-16 | 2021-12-29 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
US11015184B2 (en) | 2014-04-16 | 2021-05-25 | Codexis, Inc. | Polynucleotides encoding engineered tyrosine ammonia lyase variants |
US11807874B2 (en) | 2014-07-09 | 2023-11-07 | Codexis, Inc. | P450-BM3 variants with improved activity |
US11279917B2 (en) | 2014-07-09 | 2022-03-22 | Codexis, Inc. | P450-BM3 variants with improved activity |
US10100289B2 (en) | 2014-07-09 | 2018-10-16 | Codexis, Inc. | P450-BM3 variants with improved activity |
US10689627B2 (en) | 2014-07-09 | 2020-06-23 | Codexis, Inc. | P450-BM3 variants with improved activity |
US9708587B2 (en) | 2014-07-09 | 2017-07-18 | Codexis, Inc. | P450-BM3 variants with improved activity |
WO2016061206A1 (en) | 2014-10-16 | 2016-04-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US10370648B2 (en) | 2014-11-25 | 2019-08-06 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
US10793836B2 (en) | 2014-11-25 | 2020-10-06 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
WO2016085916A1 (en) | 2014-11-25 | 2016-06-02 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
US11802274B2 (en) | 2014-11-25 | 2023-10-31 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
US11352609B2 (en) | 2014-11-25 | 2022-06-07 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
EP4234699A1 (en) | 2014-12-22 | 2023-08-30 | Codexis, Inc. | Human alpha-galactosidase variants |
US11497798B2 (en) | 2014-12-22 | 2022-11-15 | Codexis, Inc. | Human alpha-galactosidase variants |
WO2016105889A1 (en) | 2014-12-22 | 2016-06-30 | Codexis, Inc. | Human alpha-galactosidase variants |
US10696953B2 (en) | 2015-02-10 | 2020-06-30 | Codexis, Inc. | Ketoreductase polypeptides for the synthesis of chiral compounds |
US11236308B2 (en) | 2015-02-10 | 2022-02-01 | Codexis, Inc. | Ketoreductase polypeptides for the synthesis of chiral compounds |
WO2016130412A1 (en) | 2015-02-10 | 2016-08-18 | Codexis, Inc. | Ketoreductase polypeptides for the synthesis of chiral compounds |
WO2016144688A1 (en) | 2015-03-11 | 2016-09-15 | Pioneer Hi Bred International Inc | Insecticidal combinations of pip-72 and methods of use |
US10781436B2 (en) | 2015-05-07 | 2020-09-22 | Codexis, Inc. | Penicillin-G acylases |
US11180747B2 (en) | 2015-05-07 | 2021-11-23 | Codexis, Inc. | Variant penicillin-G acylases |
US9944916B2 (en) | 2015-05-07 | 2018-04-17 | Codexis, Inc. | Penicillin-G acylases |
WO2016179464A1 (en) | 2015-05-07 | 2016-11-10 | Codexis, Inc. | Penicillin-g acylases |
US10400231B2 (en) | 2015-05-07 | 2019-09-03 | Codexis, Inc. | Penicillin-G acylases |
WO2016186986A1 (en) | 2015-05-19 | 2016-11-24 | Pioneer Hi Bred International Inc | Insecticidal proteins and methods for their use |
WO2017007547A1 (en) | 2015-07-07 | 2017-01-12 | Codexis, Inc. | Novel p450-bm3 variants with improved activity |
US10450550B2 (en) | 2015-07-07 | 2019-10-22 | Codexis, Inc. | P450-BM3 variants with improved activity |
US10113153B2 (en) | 2015-07-07 | 2018-10-30 | Codexis, Inc. | P450-BM3 variants with improved activity |
US11591578B2 (en) | 2015-07-07 | 2023-02-28 | Codexis, Inc. | P450-BM3 variants with improved activity |
US10982197B2 (en) | 2015-07-07 | 2021-04-20 | Codexis, Inc. | P450-BM3 variants with improved activity |
US10704030B2 (en) | 2015-07-07 | 2020-07-07 | Codexis, Inc. | P450-BM3 variants with improved activity |
US9683220B2 (en) | 2015-07-07 | 2017-06-20 | Codexis, Inc. | P450-BM3 variants with improved activity |
EP3943602A1 (en) | 2015-08-06 | 2022-01-26 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
WO2017023486A1 (en) | 2015-08-06 | 2017-02-09 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
WO2017105987A1 (en) | 2015-12-18 | 2017-06-22 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2017192560A1 (en) | 2016-05-04 | 2017-11-09 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3960863A1 (en) | 2016-05-04 | 2022-03-02 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2017193022A1 (en) | 2016-05-05 | 2017-11-09 | Codexis, Inc. | Penicillin-g acylases |
US11214786B2 (en) | 2016-05-05 | 2022-01-04 | Codexis, Inc. | Penicillin-G acylases |
US10724025B2 (en) | 2016-05-05 | 2020-07-28 | Codexis, Inc. | Penicillin-G acylases |
US11965192B2 (en) | 2016-05-05 | 2024-04-23 | Codexis, Inc. | Penicillin-G acylases |
US10184117B2 (en) | 2016-06-09 | 2019-01-22 | Codexis, Inc. | Biocatalysts and methods for hydroxylation of chemical compounds |
WO2017213758A1 (en) | 2016-06-09 | 2017-12-14 | Codexis, Inc. | Biocatalysts and methods for hydroxylation of chemical compounds |
US10844358B2 (en) | 2016-06-09 | 2020-11-24 | Codexis, Inc. | Polynucleotides encoding biocatalysts and methods for hydroxylation of chemical compounds |
US11634695B2 (en) | 2016-06-09 | 2023-04-25 | Codexis, Inc. | Vectors for expression of biocatalysts |
US11299723B2 (en) | 2016-06-15 | 2022-04-12 | Codexis, Inc. | Engineered beta-glucosidases and glucosylation methods |
EP3954202A1 (en) | 2016-07-01 | 2022-02-16 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
WO2018005411A1 (en) | 2016-07-01 | 2018-01-04 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
US11046936B2 (en) | 2016-08-26 | 2021-06-29 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
EP4223766A2 (en) | 2016-08-26 | 2023-08-09 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
WO2018038906A1 (en) | 2016-08-26 | 2018-03-01 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
EP4050021A1 (en) | 2016-11-01 | 2022-08-31 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2018084936A1 (en) | 2016-11-01 | 2018-05-11 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2018111551A1 (en) | 2016-12-14 | 2018-06-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2018118811A1 (en) | 2016-12-22 | 2018-06-28 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US10745681B2 (en) | 2017-01-05 | 2020-08-18 | Codexis, Inc. | Penicillin-G acylases |
US10865402B2 (en) | 2017-01-05 | 2020-12-15 | Codexis, Inc. | Penicillin-G acylases |
US11591588B2 (en) | 2017-01-05 | 2023-02-28 | Codexis, Inc. | Penicillin-G acylases |
WO2018144679A2 (en) | 2017-02-03 | 2018-08-09 | Codexis, Inc. | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
US11920167B2 (en) | 2017-02-03 | 2024-03-05 | Tate & Lyle Solutions Usa Llc | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
WO2018148001A1 (en) | 2017-02-08 | 2018-08-16 | Pioneer Hi-Bred International Inc | Insecticidal combinations of plant derived insecticidal proteins and methods for their use |
US10995329B2 (en) | 2017-02-13 | 2021-05-04 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US11161834B2 (en) | 2017-04-24 | 2021-11-02 | Tesaro, Inc. | Methods of manufacturing of niraparib |
US11629137B2 (en) | 2017-04-24 | 2023-04-18 | Tesaro, Inc. | Methods of manufacturing of niraparib |
US11021729B2 (en) | 2017-04-27 | 2021-06-01 | Codexis, Inc. | Ketoreductase polypeptides and polynucleotides |
US11746369B2 (en) | 2017-04-27 | 2023-09-05 | Codexis, Inc. | Ketoreductase polypeptides and polynucleotides |
EP3957723A2 (en) | 2017-05-08 | 2022-02-23 | Codexis, Inc. | Engineered ligase variants |
WO2018208665A1 (en) | 2017-05-08 | 2018-11-15 | Codexis, Inc. | Engineered ligase variants |
WO2018208882A1 (en) | 2017-05-11 | 2018-11-15 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US11359183B2 (en) | 2017-06-14 | 2022-06-14 | Codexis, Inc. | Engineered transaminase polypeptides for industrial biocatalysis |
US11643642B2 (en) | 2017-06-27 | 2023-05-09 | Codexis, Inc. | Penicillin-g acylases |
US10738286B2 (en) | 2017-06-30 | 2020-08-11 | Codexis, Inc. | T7 RNA polymerase variants |
US11236311B2 (en) | 2017-06-30 | 2022-02-01 | Codexis, Inc. | T7 RNA polymerase variants |
US10793841B2 (en) | 2017-06-30 | 2020-10-06 | Codexis, Inc. | T7 RNA polymerase variants |
US11236312B2 (en) | 2017-06-30 | 2022-02-01 | Codexis, Inc. | T7 RNA polymerase variants |
WO2019040335A1 (en) | 2017-08-19 | 2019-02-28 | University Of Rochester | Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents |
US11761963B2 (en) | 2017-09-27 | 2023-09-19 | Alexion Pharmaceuticals, Inc. | Biomarker signature for predicting tumor response to anti-CD200 therapy |
US11319531B2 (en) | 2017-11-07 | 2022-05-03 | Codexis, Inc. | Transglutaminase variants |
US11015180B2 (en) | 2017-12-13 | 2021-05-25 | Codexis, Inc. | Carboxyesterase polypeptides for amide coupling |
US11535833B2 (en) | 2017-12-13 | 2022-12-27 | Glaxosmithkline Intellectual Property Development Limited | Carboxyesterase biocatalysts |
WO2019116311A1 (en) | 2017-12-13 | 2019-06-20 | Glaxosmithkline Intellectual Property Development Limited | Carboxyesterase biocatalysts |
US10900055B2 (en) | 2018-06-12 | 2021-01-26 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
US11421253B2 (en) | 2018-06-12 | 2022-08-23 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
US11466259B2 (en) | 2018-07-09 | 2022-10-11 | Codexis, Inc. | Engineered galactose oxidase variant enzymes |
US10889806B2 (en) | 2018-07-09 | 2021-01-12 | Codexis, Inc. | Engineered pantothenate kinase variant enzymes |
US11845968B2 (en) | 2018-07-09 | 2023-12-19 | Codexis, Inc. | Engineered deoxyribose-phosphate aldolases |
US11795445B2 (en) | 2018-07-09 | 2023-10-24 | Codexis, Inc. | Engineered phosphopentomutase variant enzymes |
US11274286B2 (en) | 2018-07-09 | 2022-03-15 | Codexis, Inc. | Engineered deoxyribose-phosphate aldolases |
US11162105B2 (en) | 2018-07-09 | 2021-11-02 | Codexis, Inc. | Engineered purine nucleoside phosphorylase variant enzymes |
US11034948B2 (en) | 2018-07-09 | 2021-06-15 | Codexis, Inc. | Engineered phosphopentomutase variant enzymes |
US11198861B2 (en) | 2018-07-12 | 2021-12-14 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
US11760981B2 (en) | 2018-07-30 | 2023-09-19 | Codexis, Inc. | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
US11060075B2 (en) | 2018-10-29 | 2021-07-13 | Codexis, Inc. | Engineered DNA polymerase variants |
US11473077B2 (en) | 2018-12-14 | 2022-10-18 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
US11427813B2 (en) | 2018-12-20 | 2022-08-30 | Codexis, Inc. | Human alpha-galactosidase variants |
WO2021067608A1 (en) | 2019-10-02 | 2021-04-08 | Abbott Laboratories | Detection of analytes by protein switches |
US11788071B2 (en) | 2020-04-10 | 2023-10-17 | Codexis, Inc. | Engineered transaminase polypeptides |
WO2022015619A2 (en) | 2020-07-14 | 2022-01-20 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US11959108B2 (en) | 2020-08-28 | 2024-04-16 | Codexis, Inc. | Engineered protease variants |
US11767519B2 (en) | 2020-08-28 | 2023-09-26 | Codexis, Inc. | Engineered amylase variants |
WO2022125960A1 (en) | 2020-12-11 | 2022-06-16 | Willow Biosciences, Inc. | Recombinant acyl activating enzyme (aae) genes for enhanced biosynthesis of cannabinoids and cannabinoid precursors |
US11970722B2 (en) | 2020-12-18 | 2024-04-30 | Codexis, Inc. | Engineered acid alpha-glucosidase variants |
US11898174B2 (en) | 2020-12-18 | 2024-02-13 | Codexis, Inc. | Engineered uridine phosphorylase variant enzymes |
WO2022212824A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered adenylate kinase variant enzymes |
WO2022212835A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered acetate kinase variant enzymes |
WO2022212832A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered cyclic gmp-amp synthase (cgas) variant enzymes |
US11913036B2 (en) | 2021-04-02 | 2024-02-27 | Codexis, Inc. | Engineered acetate kinase variant enzymes |
US11913037B2 (en) | 2021-04-02 | 2024-02-27 | Codexis, Inc. | Engineered adenylate kinase variant enzymes |
WO2022212828A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered guanylate kinase variant enzymes |
WO2023010083A2 (en) | 2021-07-30 | 2023-02-02 | Willow Biosciences, Inc. | Recombinant prenyltransferase polypeptides engineered for enhanced biosynthesis of cannabinoids |
WO2023023621A1 (en) | 2021-08-19 | 2023-02-23 | Willow Biosciences, Inc. | Recombinant olivetolic acid cyclase polypeptides engineered for enhanced biosynthesis of cannabinoids |
WO2023069921A1 (en) | 2021-10-19 | 2023-04-27 | Epimeron Usa, Inc. | Recombinant thca synthase polypeptides engineered for enhanced biosynthesis of cannabinoids |
WO2023173084A1 (en) | 2022-03-11 | 2023-09-14 | University Of Rochester | Cyclopeptibodies and uses thereof |
WO2024040020A1 (en) | 2022-08-15 | 2024-02-22 | Absci Corporation | Quantitative affinity activity specific cell enrichment |
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