WO1995022279A1

WO1995022279A1 - Videographic method for detecting cancer

Info

Publication number: WO1995022279A1
Application number: PCT/US1994/001918
Authority: WO
Inventors: Joseph Hines
Original assignee: Zila Pharmaceuticals, Inc.
Priority date: 1994-02-16
Filing date: 1994-02-16
Publication date: 1995-08-24
Also published as: JPH09500048A; AU6813094A; EP0693903A1; EP0693903A4; KR960701614A

Abstract

A method for detecting epithelial cancer includes generating a real-time video image of the locus of potential cancerous sites on the epithelium. The real-time video image is displayed on a television monitor screen. The real-time video image on the monitor is examined to locate suspected cancerous sites and the results of such examination are recorded.

Description

Videographic Method for Detecting Cancer

This invention relates to methods for detecting epithelial cancer.

More particularly, the invention relates to a cancer detection method employing video technology for real-time examination of potential cancerous sites on the epithelium.

In another aspect, the invention relates to real¬ time videographic examination techniques in combination with in vivo tissue staining techniques for the diagnosis of epithelial cancer.

In an even further and still more particular respect, the invention relates to cancer detection/diagnosis techniques in which videographic records of the visual image of a suspected cancer site on the epithelium are compared with a real-time videographic image of the suspected site which is made at a later time.

According to another aspect, the invention pertains to therapeutic methods, e.g., surgical excision, in which the delineation of the cancerous tissue is aided by reference to videographic records of prior examinations of the locus of the cancer.

Visual examination of epithelial tissue to detect cancerous or pre-cancerous tissue is known. In vivo examination of suspected cancerous sites using standard photographs, magnification by an operating microscope (stomatoscopy) and color photomicrographs for the detection of oral cancer was described in the article by Shedd et al . , American Journal of Surgery, Volume 110, pages 631 et seq. (October 1965) . Dental television cameras which facilitate visualization of the oral cavity are also well known. See, e.g., the U.S. Patents Nos. 4,260,376 to Litel; 4,195,626 to Lemmev and 5,251,023 to Cooper. Apparatus and methods for recording in vivo holograms and for reconstructing such recorded holograms for later display on a television monitor were described by Bielkhagen, et al. in U.S. Patent 5,014,709.

The use of biological stains for in vivo selective delineation of cancerous and pre-cancerous tissue is also well known. See, e.g., U.S. Patent 4,321,251 to Mashberg and the article by Nibel (Journal of American Dental Association, Volume 35, pages 801 et seq. (June, 1964)). Shedd, supra, discloses the use of selective tissue staining techniques in combination with stomoatoscopy.

Cancer of the oral mucosa is one of the leading terminal diseases causing thousands of deaths annually in the United States alone. Oral cancers occur at various places in the oral cavity, including the floor of the mouth, the anterior pillars, on the pallet and on the bottom and the dorsum of the tongue. Most oral cancers are curable by surgical excision if detected early enough. However, the difficulty of visualizing the various intraoral epithelial surfaces makes it difficult to detect oral cancers in the very early stages. It is particularly difficult to detect pre-cancerous sites in the oral cavity, because of the difficulty in visualizing the physically remote oral epithelium coupled with the confusingly similar appearance of precancerous tissues with tissue having other, non-cancerous conditions.

While video technology and equipment has been available for a number of years which enables dental practitioners to visualize intraoral tissue in order to facilitate conventional dental procedures and while tissue staining techniques have been known for many years which selectively stain and visually delineate cancerous epithelial tissue and plaque and while photographic, holographic and other techniques have been known for recording visual images for later examination, such techniques have not been effectively employed to provide straightforward real-time diagnostic visual examination of the intraoral epithelium for detection of oral cancers and pre-cancerous conditions.

It would be highly desirable to provide improved methods for detecting epithelial cancers, particularly oral cancers.

It would also be advantageous to provide such methods which can be effectively employed by general dental practitioners as a part of routine dental procedures or "check-ups" or as an adjunct to routine periodic dental office visits for plaque removal and the like. Such improved oral cancer detection methods could result in early detection of oral cancer and timely therapy, saving many thousands of lives annually.

Accordingly, the principal object of the present invention is to provide an improved method for early detection of epithelial cancer, particularly oral cancers, including single or multiple small or large sites.

Yet another object of the invention is to provide such improved methods which can be used effectively by general dental practitioners, with a minimum of additional training and expense, to provide routine oral cancer screening services as an adjunct to routine dental procedures and examinations. Still another object of the invention is to provide oral cancer screening techniques which permit real-time examination of remotely located epithelial tissues in the oral cavity, which are difficult to examine by conventional visual examination techniques.

Still another and further object of the invention is to provide real-time cancer detection techniques which, nevertheless, provide a permanent record of the results of such an examination, so as to permit comparative examination of the present image of a suspected cancer site with images of the same site made at an earlier time.

A still further object of the invention is to provide an epithelial cancer detection and delineation method which provides a visual record for later reference during therapy, e.g., to aid in excision surgery.

Those and other, further and more specific objects and advantages of the invention will be apparent to those skilled in the art from the following detailed description thereof, taken in conjunction with the drawing in which:

Fig. 1 is a flow sheet illustrating various embodiments of the method of the present invention, and the best mode presently known for the practice thereof.

Briefly, in accordance with the invention, I provide a method for detecting epithelial cancer which includes the steps of generating video signals which represent the real-time image of the locus of potential cancerous sites on the epithelium, displaying this real-time image on a television monitor screen, visually examining the displayed image to locate suspected cancerous sites and recording the results of such examination. In a preferred embodiment of the invention, the recording step is carried out by electronically storing the video signals. In a further preferred embodiment of the invention, the method also includes applying a biological stain to the epithelium, to selectively delineate cancerous tissue. In the one embodiment, the stain is applied to the epithelium in the locus of the suspected cancerous sites. In another embodiment the stain is applied generally, for example, in the case of the oral epithelium, the stain may be applied as a mouth wash, according to the procedures generally disclosed in the Mashberg Patent, U.S. 4,321,251. Other aspects of the best mode presently contemplated for practicing the present invention are disclosed in connection with the description of Fig. 1, set forth below.

The drawing is presented for purposes of illustrating the preferred practice of the invention, but is not intended as a limitation on the scope of the invention.

Referring to Fig. 1, according to my method a conventional dental video camera is used to make a preliminary scan 10 of the tissue which is the locus of potential cancerous sites on the epithelium. For example, a suitable video camera for use in accordance with my invention is disclosed in the patent to Cooper, et al. U.S. 5,241,025. The video image 11 from the scan 10 may be subjected to electronic image processing 12 (as indicated by the arrow 11a) or the image 11 can be directly utilized as indicated by the arrow lib. Either the process image 12a or the unprocessed image lib is then displayed for visual examination 13 on a television monitor screen. If such visual examination 13 definitively reveals a cancerous site, as indicated by the arrow 13a, this may be directly employed to effect a diagnosis 14.

On the other hand, if the visual examination 13 does not yield a definitive diagnosis 14, but indicates suspect cancerous sites (as indicated by the arrow 13b) , then a selective stain is applied 15 to the suspect sites and a second video scan 16 of the stained sites is made. For example, such selective stain compositions are included in diagnostic kits which are commercially available in certain countries under the trademark "ORASCAN" . The image from the second scan 16 is then either subjected to image processing as indicated by the arrow 16a or the unprocessed image 16b is displayed on a television monitor for visual examination 13. The visual examination of the processed or unprocessed image from the second scan 16 may also lead directly to a diagnosis 14.

The video signals representing the images formed by either the first scan 10 or the second scan 16, processed or unprocessed, are recorded 17, for example by a conventional video recorder, as indicated by the arrows 17a, 17b and 17c and the resultant records 17d are stored 18 for future reference.

Any of the visual examinations 13 may be facilitated by comparisons 19 of the real-time image 13c and a previously recorded stored image 18a of the same site to facilitate diagnosis 14, as indicated by the arrow 19a. Similarly, a visual examination 13 may indicate the necessity or desirability of a biopsy 21 as indicated by the arrow 13d, to confirm the diagnosis 14 as indicated by the arrow 21a. If the diagnosis 14 indicates that therapy is required as indicated by the arrow 14a, the therapy, for example, surgery excision can be facilitated, as indicated by the arrow 19b by a comparison 19 of the real-time image 13c with a stored image 18a or by direct reference to the stored image 18b.

As used herein, the term "cancerous site" is intended to include both cancerous and precancerous tissues. The cancerous tissue may be in si tu carcinomas or early-invasive carcinomas and the precancerous conditions include dysplastic tissues or lesions and neoplastic tissues or lesions as distinguished from the normal squamous epithelium.

The term "image processing" includes any of the known electronic image intensification techniques, including as for example, digital filtering, contrast enhancement, frequency analysis, 3-D rotation, digital magnification, threshold analysis and the like.

Having described my invention in such terms as to enable those skilled in the art to understand and practice it and having identified the presently preferred embodiments and best mode thereof, I claim:

Claims

CLAIM

1. A method for detecting epithelial cancer comprising:

a) generating video signals representing the real¬ time image of the locus of potential cancerous sites on the epithelium;

b) displaying said real-time image on a television monitor screen;

c) visually examining said displayed image to locate suspected cancerous sites; and

d) recording the results of such examination.

2. The method of Claim 1 in which said recording step is carried out by electronically storing said video signals.

3. The method of Claim 1 which includes the step of applying a biological stain to the epithelium to selectively delineate cancerous tissue.

4. The method of Claim 3 in which said stain is applied in the locus of said suspected cancerous sites.

5. The method of Claim 4 in which said visual examination is a first visual examination to identify said suspected cancerous sites.

6. The method of Claim 5 which includes the step of a second visual examination of said suspected cancerous sites after the application of said biological stain.

7. The method of Claim 1 which includes the step of electronically processing such video signals to visually enhance said suspected sites.

8. The method of Claim 1 in which the image represented by said stored video signals is compared with a real-time image of said locus.