WO1995020620A1 - Procedure for production of spherical gel particles - Google Patents

Procedure for production of spherical gel particles Download PDF

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Publication number
WO1995020620A1
WO1995020620A1 PCT/SE1995/000053 SE9500053W WO9520620A1 WO 1995020620 A1 WO1995020620 A1 WO 1995020620A1 SE 9500053 W SE9500053 W SE 9500053W WO 9520620 A1 WO9520620 A1 WO 9520620A1
Authority
WO
WIPO (PCT)
Prior art keywords
polymer
particles
solvent
jelling
procedure according
Prior art date
Application number
PCT/SE1995/000053
Other languages
French (fr)
Inventor
Ingmar Jonsson
Original Assignee
Bio Dev Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Dev Ab filed Critical Bio Dev Ab
Publication of WO1995020620A1 publication Critical patent/WO1995020620A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00—Processes of treating or compounding macromolecular substances
    • C08J3/12—Powdering or granulating
    • C08J3/14—Powdering or granulating by precipitation from solutions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2/00—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic
    • B01J2/02—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic by dividing the liquid material into drops, e.g. by spraying, and solidifying the drops
    • B01J2/06—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic by dividing the liquid material into drops, e.g. by spraying, and solidifying the drops in a liquid medium
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2300/00—Characterised by the use of unspecified polymers
    • C08J2300/14—Water soluble or water swellable polymers, e.g. aqueous gels

Definitions

  • This invention concerns the production of spherical jelled and/or polymerized particles. More precise is the invention concerned with a method for production of spherical particles for chromatography applications.
  • polymerized and/or jelled spherical particles constitute the chromatographic separation gel bed.
  • matrices produced through a dispersion process, based on extensive mechanical agitation of the normally watersolouble matter, which is to be dispersed in a dispersion medium, which in turn usually is constituted of an organic solvent, such as toluene.
  • the generated spherical particles are processed in several steps including sieving and thorough cleansing from the organic solvent employed.
  • the purpose of this invention is to remove the above mentioned drawbacks of the generally known process and furthermore produce improved spherical particles for chromatography.
  • spherical, jelled particles produced through that polymerisable and/or jelling polymers in liquid form or in solution by mechanical means is disintegrated into a spherical shape characterized by a narrow particle size distribution, whereupon the so obtained particles are transported through a gas, normally atmospheric air, are further transported into a solvent, in which the applied polymer is essentially soluble, but which is held at a temperature below the jelling point of the respective polymer.
  • the procedure can be applied to any natural or synthetic polymer, but can with special advantage be used for agarose or gelatine, which are gelatinized bye
  • the mechanical dispersion of the polymer/polymer solution into spherical droplets with narrow particle size distribution is preferably performed by the device, described in the application for a patent PCT/SE88/00145.
  • the droplets can suitably be produced with a particle diameter between 20 and 500 ⁇ m. Batch variations are typically less than +/- 25%.
  • the polymer dissolved in water which also with advantage may constitute the solvent, to which the polymer is transferred for completion of the jelling.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Preparation (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)

Abstract

A method for producing spherical, jelled particles in which jelling liquid polymer pure or in solution by mechanical means is dispersed into spherically shaped particles with a narrow particle size distribution, after which the received particles after transport through gas, preferably air, are transferred into a solvent, in which the polymer is essentially soluble, at which the solvent has a temperature beneath the jelling point of the polymer. The produced jelled particles may finally be separated from the solvent.

Description

Procedure for production of spherical gel particles
This invention concerns the production of spherical jelled and/or polymerized particles. More precise is the invention concerned with a method for production of spherical particles for chromatography applications.
For the separation of macromolecules using chromatography porous, polymerized and/or jelled spherical particles constitute the chromatographic separation gel bed. Conventionally are such matrices produced through a dispersion process, based on extensive mechanical agitation of the normally watersolouble matter, which is to be dispersed in a dispersion medium, which in turn usually is constituted of an organic solvent, such as toluene. The generated spherical particles are processed in several steps including sieving and thorough cleansing from the organic solvent employed.
The demands upon cleansing especially from the organic solvent employed in the process are rigorous and it is also from an environmental point of view very important to find improved processes in which organic solvents can be avoided. The conventional emulsion procedure for the production of particles for chromatography is costly and unfit from an environmental point of view, the productivity is low, it yields of wide particle size distribution, it is a non continuous and an energy demanding process.
The purpose of this invention is to remove the above mentioned drawbacks of the generally known process and furthermore produce improved spherical particles for chromatography.
According to the invention are spherical, jelled particles produced through that polymerisable and/or jelling polymers in liquid form or in solution by mechanical means is disintegrated into a spherical shape characterized by a narrow particle size distribution, whereupon the so obtained particles are transported through a gas, normally atmospheric air, are further transported into a solvent, in which the applied polymer is essentially soluble, but which is held at a temperature below the jelling point of the respective polymer.
In principle the procedure can be applied to any natural or synthetic polymer, but can with special advantage be used for agarose or gelatine, which are gelatinized bye
CONFIRMATION COPY cooling. The mechanical dispersion of the polymer/polymer solution into spherical droplets with narrow particle size distribution is preferably performed by the device, described in the application for a patent PCT/SE88/00145. The droplets can suitably be produced with a particle diameter between 20 and 500 μm. Batch variations are typically less than +/- 25%. Further is the polymer dissolved in water, which also with advantage may constitute the solvent, to which the polymer is transferred for completion of the jelling.
Upor 'hat the spherical particles are transferred they are allowed to fall through a gas
The procedure of the invention may by illustrated by the example below.
4-6 % (w/v) Agarose is added to boiling water and is allowed to cool. The solution is desintegrated into spherical particles by the device described in PCT/SE88/00145. The particles are allowed to a free gravity fall through air. Finally are the particles captured in a basin of water as the solvent at a suitable temperature, leading to complete jelling. The so jelled spherical particles are then separated through filtration and/or sedimentation.

Claims

Patent Claims
1. Method for the production of spherical particles of jelling liquid polymers or jelling polymers dissolved in a solvent of narrow particle size distribution, characterized by that the so obtained particles via transport in gas, preferably air, are transfeπed to a solvent, in which the polymer is essentially soluble, at which the solvent has a temperature below the jelling point of the polymer.
2. Procedure according to Claim 1, characterized by that the gas has such temperature that a suitable cooling of the particles is achieved.
3. Procedure according to Claim 2, characterized by that the polymer is a polysacharide.
4. Procedure according to Claim 3, charcterized by that the polymer is agarose or starch.
5. Procedure according to Claim 4, charcterized by that the polymer is gelatine.
6. Procedure according to Claim 1-5, characterized by that the polymer is dissolved in water and that the receiving solvent is water.
7. Procedure according to Claim 1-6, characterized by that the spherical particles fall through air.
8. Procedure according to Claim 1-7, characterized by that the jelling polymer is agarose, and constitutes a water solution containing 2-10% agarose.
PCT/SE1995/000053 1994-01-28 1995-01-20 Procedure for production of spherical gel particles WO1995020620A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9400261A SE9400261D0 (en) 1994-01-28 1994-01-28 Process for the preparation of spherical gelled particles
SE9400261-5 1994-01-28

Publications (1)

Publication Number Publication Date
WO1995020620A1 true WO1995020620A1 (en) 1995-08-03

Family

ID=20392716

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE1995/000053 WO1995020620A1 (en) 1994-01-28 1995-01-20 Procedure for production of spherical gel particles

Country Status (2)

Country Link
SE (1) SE9400261D0 (en)
WO (1) WO1995020620A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001040767A1 (en) * 1999-12-01 2001-06-07 Ap Biotech Ab Method of producing porous spherical particles
US6984733B2 (en) * 2000-12-29 2006-01-10 Amersham Biosciences Ab Manufacturing process for porous material

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0241299A2 (en) * 1986-04-11 1987-10-14 Keltcrest Limited Masterbatch production
WO1993002785A1 (en) * 1991-07-30 1993-02-18 Nukem Gmbh Process and plant for the production of spherical alginate pellets

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0241299A2 (en) * 1986-04-11 1987-10-14 Keltcrest Limited Masterbatch production
WO1993002785A1 (en) * 1991-07-30 1993-02-18 Nukem Gmbh Process and plant for the production of spherical alginate pellets

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001040767A1 (en) * 1999-12-01 2001-06-07 Ap Biotech Ab Method of producing porous spherical particles
JP2003517509A (en) * 1999-12-01 2003-05-27 アメルシャム・バイオサイエンシーズ・アクチボラグ Method for producing porous spherical particles
US6841097B2 (en) 1999-12-01 2005-01-11 Amersham Biosciences Ab Method of producing porous spherical particles
JP5000059B2 (en) * 1999-12-01 2012-08-15 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ Method for producing porous spherical particles
US6984733B2 (en) * 2000-12-29 2006-01-10 Amersham Biosciences Ab Manufacturing process for porous material

Also Published As

Publication number Publication date
SE9400261D0 (en) 1994-01-28

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