WO1995011309A3 - A novel nuclear mitotic phosphoprotein: mitosin - Google Patents

A novel nuclear mitotic phosphoprotein: mitosin Download PDF

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Publication number
WO1995011309A3
WO1995011309A3 PCT/US1994/012162 US9412162W WO9511309A3 WO 1995011309 A3 WO1995011309 A3 WO 1995011309A3 US 9412162 W US9412162 W US 9412162W WO 9511309 A3 WO9511309 A3 WO 9511309A3
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WO
WIPO (PCT)
Prior art keywords
mitosin
protein
nucleic acid
phase
acid molecule
Prior art date
Application number
PCT/US1994/012162
Other languages
French (fr)
Other versions
WO1995011309A2 (en
Inventor
Xeuliang Zhu
Wen-Hwa Lee
Original Assignee
Univ Texas
Xeuliang Zhu
Lee Wen Hwa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas, Xeuliang Zhu, Lee Wen Hwa filed Critical Univ Texas
Priority to SK489-96A priority Critical patent/SK48996A3/en
Priority to EP94932002A priority patent/EP0723595A1/en
Priority to JP7512251A priority patent/JPH09507744A/en
Priority to BR9407874A priority patent/BR9407874A/en
Priority to AU80885/94A priority patent/AU8088594A/en
Publication of WO1995011309A2 publication Critical patent/WO1995011309A2/en
Publication of WO1995011309A3 publication Critical patent/WO1995011309A3/en
Priority to FI961665A priority patent/FI961665A/en
Priority to NO961571A priority patent/NO961571L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4738—Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/02—Drugs for disorders of the urinary system of urine or of the urinary tract, e.g. urine acidifiers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • Endocrinology (AREA)
  • Reproductive Health (AREA)
  • Dermatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A novel purified phosphoprotein designated mitosin is provided by this invention. Also provided is the amino acid sequence and nucleic acid molecule corresponding to mitosin protein. Diagnostic and therapeutic methods using the protein and nucleic acid molecule also are provided. A nucleic acid molecule encoding mitosin also is provided by this invention, as well as active fragments thereof. The nucleic acid molecules are useful to recombinantly produce mitosin and for use as probes. The compositions and methods of this invention are based on the instant discovery that the intracellular presence of mitosin is necessary for a eukaryotic cell to enter into the M phase of mitosis, and that the degradation of mitosin is necessary for the cell to advance to the next stage. Thus, an anti-mitosin antibody, a mutant or a non-functional analog of mitosin would inhibit the mitotic cell cycle by preventing cells from entering the M phase, and overexpression of mitosin, or a functional equivalent thereof, would inhibit the cycle by preventing cells from leaving the M phase. Such overexpression could be achieved either by addition of the protein or through gene therapy, i.e. delivery of a gene encoding the protein or a functional equivalent thereof.
PCT/US1994/012162 1993-10-22 1994-10-24 A novel nuclear mitotic phosphoprotein: mitosin WO1995011309A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
SK489-96A SK48996A3 (en) 1993-10-22 1994-10-24 Novel nuclear mitotic phosphoprotein: mitosin
EP94932002A EP0723595A1 (en) 1993-10-22 1994-10-24 A novel nuclear mitotic phosphoprotein: mitosin
JP7512251A JPH09507744A (en) 1993-10-22 1994-10-24 A novel fission phosphoprotein: mitocin
BR9407874A BR9407874A (en) 1993-10-22 1994-10-24 Purified mammalian protein called mitosin biologically active fragment purified mammalian protein isolated nucleic acid molecule expression vector host vector system for production of mitosin antimitosin antibody and process for production of mitosin
AU80885/94A AU8088594A (en) 1993-10-22 1994-10-24 A novel nuclear mitotic phosphoprotein: mitosin
FI961665A FI961665A (en) 1993-10-22 1996-04-16 New mitotic nuclear phosphoprotein: mitocin
NO961571A NO961571L (en) 1993-10-22 1996-04-19 New mitotic nuclear phosphoprotein: mitosine

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14123993A 1993-10-22 1993-10-22
US08/141,239 1993-10-22

Publications (2)

Publication Number Publication Date
WO1995011309A2 WO1995011309A2 (en) 1995-04-27
WO1995011309A3 true WO1995011309A3 (en) 1995-06-08

Family

ID=22494806

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/012162 WO1995011309A2 (en) 1993-10-22 1994-10-24 A novel nuclear mitotic phosphoprotein: mitosin

Country Status (14)

Country Link
US (1) US5710022A (en)
EP (1) EP0723595A1 (en)
JP (1) JPH09507744A (en)
CN (1) CN1133616A (en)
AU (1) AU8088594A (en)
BR (1) BR9407874A (en)
CA (1) CA2173978A1 (en)
CZ (1) CZ111996A3 (en)
FI (1) FI961665A (en)
HU (1) HUT74839A (en)
NO (1) NO961571L (en)
PL (1) PL314179A1 (en)
SK (1) SK48996A3 (en)
WO (1) WO1995011309A2 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7223842B1 (en) 1986-08-11 2007-05-29 Massachusetts Eye And Ear Infirmary Detection of proteins whose absence is associated with a neoplasm
US7384735B1 (en) 1986-08-11 2008-06-10 Massachusetts Eye And Ear Infirmary Retinoblastoma nucleic acids
US20030086927A1 (en) * 1995-11-01 2003-05-08 University Of Southern California Expression of cyclin G1 in tumors
ES2303726T3 (en) * 1997-04-10 2008-08-16 University Of Southern California MODIFIED PROTEINS THAT ARE FIXED TO COMPONENTS OF THE EXTRACELLULAR MATRIX.
US6746676B1 (en) * 1998-04-20 2004-06-08 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University Chlamydia proteins and their uses
US20060292121A1 (en) * 1998-04-29 2006-12-28 Hall Frederick L Retroviral vectors including modified envelope escort protein
US6876760B1 (en) * 2000-12-04 2005-04-05 Cytokinetics, Inc. Classifying cells based on information contained in cell images
US7151847B2 (en) * 2001-02-20 2006-12-19 Cytokinetics, Inc. Image analysis of the golgi complex
NZ516738A (en) * 1999-07-20 2004-01-30 Univ Southern California Identification of pluripotent pre-mesenchymal, pre-hematopoietic progenitor cells expressing a unique molecular marker
WO2002044394A2 (en) 2000-11-29 2002-06-06 University Of Southern California Targetet retoviral vectors for cancer immunotherapy
US7218764B2 (en) * 2000-12-04 2007-05-15 Cytokinetics, Inc. Ploidy classification method
US7016787B2 (en) * 2001-02-20 2006-03-21 Cytokinetics, Inc. Characterizing biological stimuli by response curves
US6956961B2 (en) * 2001-02-20 2005-10-18 Cytokinetics, Inc. Extracting shape information contained in cell images
US20040071328A1 (en) * 2001-09-07 2004-04-15 Vaisberg Eugeni A. Classifying cells based on information contained in cell images
AU2003235316A1 (en) * 2002-08-23 2004-03-11 Japan Science And Technology Agency Human solid cancer antigen peptides, polynucleotides encoding the same and utilization thereof
US20070178066A1 (en) * 2003-04-21 2007-08-02 Hall Frederick L Pathotropic targeted gene delivery system for cancer and other disorders
CA2538495A1 (en) * 2003-09-15 2005-03-24 Cenix Bioscience Gmbh The use of eukaryotic genes affecting chromatin separation for diagnosis and treatment of proliferative diseases
US20050273271A1 (en) * 2004-04-05 2005-12-08 Aibing Rao Method of characterizing cell shape
US7323318B2 (en) * 2004-07-15 2008-01-29 Cytokinetics, Inc. Assay for distinguishing live and dead cells
US20070031818A1 (en) * 2004-07-15 2007-02-08 Cytokinetics, Inc., A Delaware Corporation Assay for distinguishing live and dead cells
US20100093767A1 (en) * 2004-12-03 2010-04-15 Takeda San Diego, Inc. Mitotic Kinase Inhibitors
WO2008137114A1 (en) * 2007-05-04 2008-11-13 University Of Hawai'i Methods and compositions for targeted delivery of gene therapeutic vectors
US9291425B2 (en) * 2013-05-24 2016-03-22 Patrol Incident Gear, Llc. Weapon sling
CN113671197B (en) * 2021-08-19 2023-08-08 四川沃文特生物技术有限公司 Buffer solution, calibrator, kit, preparation method and application

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06296489A (en) * 1992-11-20 1994-10-25 Univ Texas Syst Cellular gene for coding retinoblastoma-related protein

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BUCHKOVICH ET AL.: "The retinoblastoma protein is phosphorylated during specific phases of the cell cycle", CELL, vol. 58, 22 September 1989 (1989-09-22), pages 1097 - 1105 *
SHAN ET AL.: "Molecular cloning of cellular genes encoding retinoblastoma-associated proteins : identification of a gene with properties of the transcription factor E2F", MOL. CELL. BIOL., vol. 12, no. 12, December 1992 (1992-12-01), pages 5620 - 5631 *

Also Published As

Publication number Publication date
US5710022A (en) 1998-01-20
WO1995011309A2 (en) 1995-04-27
CN1133616A (en) 1996-10-16
NO961571D0 (en) 1996-04-19
CA2173978A1 (en) 1995-04-27
NO961571L (en) 1996-06-24
BR9407874A (en) 1996-10-29
JPH09507744A (en) 1997-08-12
FI961665A0 (en) 1996-04-16
HU9601023D0 (en) 1996-06-28
PL314179A1 (en) 1996-08-19
SK48996A3 (en) 1996-10-02
FI961665A (en) 1996-06-06
HUT74839A (en) 1997-02-28
CZ111996A3 (en) 1996-10-16
AU8088594A (en) 1995-05-08
EP0723595A1 (en) 1996-07-31

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