WO1993007486A1 - Method of obtaining trophoblast cells from a foetus - Google Patents

Method of obtaining trophoblast cells from a foetus Download PDF

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Publication number
WO1993007486A1
WO1993007486A1 PCT/FR1992/000950 FR9200950W WO9307486A1 WO 1993007486 A1 WO1993007486 A1 WO 1993007486A1 FR 9200950 W FR9200950 W FR 9200950W WO 9307486 A1 WO9307486 A1 WO 9307486A1
Authority
WO
WIPO (PCT)
Prior art keywords
trophoblast cells
antibody
foetus
leucocytes
separation
Prior art date
Application number
PCT/FR1992/000950
Other languages
French (fr)
Inventor
Christophe Julien
Emile Papiernik-Berkauer
Original Assignee
Société Civile Particulière De Saint-Cyr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Société Civile Particulière De Saint-Cyr filed Critical Société Civile Particulière De Saint-Cyr
Publication of WO1993007486A1 publication Critical patent/WO1993007486A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells

Abstract

Method of obtaining trophoblast cells from a foetus of a mammal, specifically from a sample of a the peripheral blood taken from the mother bearing the said foetus, involving the following sequence of steps: (a) separation of erythrocytes from the said sample; (b) separation of at least part of the leucocytes, marking at least part of the leucocytes with at least a first specific marker; (c) separation of the trophoblast cells, marking the latter with at least one second antibody substantially specific to the said cells; (d) positive selection of the trophoblast cells by flow cytometry. The method is characterized in that the first antibody of the pre-enrichement step is a common antileucocyte antibody, specifically marking 80 % of the leucocytes of the leucocyte fraction, to give a fraction pre-enriched with trophoblast cells, enabling the trophoblast cells marked only by the second antibody to be identified subsequently during flow cytometry.
PCT/FR1992/000950 1991-10-11 1992-10-09 Method of obtaining trophoblast cells from a foetus WO1993007486A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9112785A FR2682481A1 (en) 1991-10-11 1991-10-11 PROCESS FOR OBTAINING TROPHOBLASTIC CELLS FROM A FÓOETUS.
FR91/12785 1991-10-11

Publications (1)

Publication Number Publication Date
WO1993007486A1 true WO1993007486A1 (en) 1993-04-15

Family

ID=9417999

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1992/000950 WO1993007486A1 (en) 1991-10-11 1992-10-09 Method of obtaining trophoblast cells from a foetus

Country Status (3)

Country Link
AU (1) AU2893792A (en)
FR (1) FR2682481A1 (en)
WO (1) WO1993007486A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994025873A1 (en) * 1993-04-23 1994-11-10 Cellpro, Incorporated Methods for enriching fetal progenitor cells from maternal blood
FR2751989A1 (en) * 1996-08-01 1998-02-06 Soc D Rech Et De Dev En Activa METHOD OF IDENTIFYING EUKARYOTIC CELLS BY FIXING THE SAID CELLS ON A SUPPORT AND SUPPORT FOR IMPLEMENTING THE SAID METHOD
US6355174B1 (en) 1994-11-08 2002-03-12 Phoenix Medical Limited Method of separating foetal trophoblasts from maternal blood

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0341966A2 (en) * 1988-05-10 1989-11-15 The Board Of Trustees Of The Leland Stanford Junior University Homogeneous mammalian hematopoietic stem cell composition
WO1990006509A1 (en) * 1988-12-06 1990-06-14 Flinders Technologies Pty. Ltd. Isolation of fetal cells from maternal blood to enable prenatal diagnosis
EP0412700A1 (en) * 1989-08-03 1991-02-13 The Regents Of The University Of California Method for isolating fetal cytotrophoblast cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0341966A2 (en) * 1988-05-10 1989-11-15 The Board Of Trustees Of The Leland Stanford Junior University Homogeneous mammalian hematopoietic stem cell composition
WO1990006509A1 (en) * 1988-12-06 1990-06-14 Flinders Technologies Pty. Ltd. Isolation of fetal cells from maternal blood to enable prenatal diagnosis
EP0412700A1 (en) * 1989-08-03 1991-02-13 The Regents Of The University Of California Method for isolating fetal cytotrophoblast cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
PRENATAL DIAGNOSIS vol. 11, no. 10, Octobre 1991, CHICHESTER pages 787 - 798 J.F.BRUCH ET AL 'Trophoblast-like cells sorted from peripheral maternal blood using flow cytometry: a multiparametric study involving transmission electron microscopy and fetal DNA amplification' *
PRENATAL DIAGNOSIS vol. 8, 1988, CHICHESTER pages 591 - 607 A.E.COVONE ET AL. 'Analysis of periheral maternal blood samples for the presence of placenta-derived cells using Y-specific probes and McAB H315' cité dans la demande *
THE LANCET vol. II, no. 8407, 13 Octobre 1984, LONDON pages 841 - 843 A.E.COVONE ET AL 'Trophoblast cells in peripheral blood from pregnant women' cité dans la demande *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994025873A1 (en) * 1993-04-23 1994-11-10 Cellpro, Incorporated Methods for enriching fetal progenitor cells from maternal blood
US6355174B1 (en) 1994-11-08 2002-03-12 Phoenix Medical Limited Method of separating foetal trophoblasts from maternal blood
FR2751989A1 (en) * 1996-08-01 1998-02-06 Soc D Rech Et De Dev En Activa METHOD OF IDENTIFYING EUKARYOTIC CELLS BY FIXING THE SAID CELLS ON A SUPPORT AND SUPPORT FOR IMPLEMENTING THE SAID METHOD
WO1998005965A1 (en) * 1996-08-01 1998-02-12 Societe De Recherche Et De Developpement En Activation Et Communication Cellulaire Method for identifying eukaryotic cells by fixing said cells on a support and support for implementing said method

Also Published As

Publication number Publication date
FR2682481A1 (en) 1993-04-16
AU2893792A (en) 1993-05-03

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