WO1989007880A3 - Process for producing biochemicals - Google Patents

Process for producing biochemicals Download PDF

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Publication number
WO1989007880A3
WO1989007880A3 PCT/US1989/000180 US8900180W WO8907880A3 WO 1989007880 A3 WO1989007880 A3 WO 1989007880A3 US 8900180 W US8900180 W US 8900180W WO 8907880 A3 WO8907880 A3 WO 8907880A3
Authority
WO
WIPO (PCT)
Prior art keywords
tube
compartment
fluid
segregated
gas
Prior art date
Application number
PCT/US1989/000180
Other languages
French (fr)
Other versions
WO1989007880A2 (en
Inventor
Dennis D Pietronigro
Robert J Ruemer
John L Sternick
Original Assignee
Nygene Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nygene Corp filed Critical Nygene Corp
Publication of WO1989007880A2 publication Critical patent/WO1989007880A2/en
Publication of WO1989007880A3 publication Critical patent/WO1989007880A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

This process utilizes a number of small volume compartments containing culture medium (22). Each compartment (22) be enclosed in a tube, preferably transparent, and is segregated in the tube with the aid of a gas-permeable carrier fluid (60). Movement of fluid in a given compartment along the tube to a detection station (40) is often practical. If the fluid in a given compartment is found to contain components desirable for further processing, it can be segregated for such processing. The process is particularly valuable in production of monoclonal antibodies and in use wherein gas-transfer is required to or from the reaction compartment. However, the invention can also facilitate intracellular studies, e.g. pH and calcium content, and studies of the properties of sites on the cell membrane.
PCT/US1989/000180 1988-02-10 1989-01-24 Process for producing biochemicals WO1989007880A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15486588A 1988-02-10 1988-02-10
US154,865 1988-02-10

Publications (2)

Publication Number Publication Date
WO1989007880A2 WO1989007880A2 (en) 1989-09-08
WO1989007880A3 true WO1989007880A3 (en) 1989-10-05

Family

ID=22553137

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1989/000180 WO1989007880A2 (en) 1988-02-10 1989-01-24 Process for producing biochemicals

Country Status (3)

Country Link
CN (1) CN1048062A (en)
AU (1) AU3182289A (en)
WO (1) WO1989007880A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69027961T2 (en) * 1989-09-06 1997-01-09 Takeda Chemical Industries Ltd Protein, DNA and their use
CA2557574C (en) 2004-02-23 2013-04-30 Eudes Francois Marie De Crecy Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants
EP2807255B1 (en) * 2012-01-25 2017-08-02 Gencell Biosystems Limited Biomolecule isolation
WO2016004015A1 (en) * 2014-06-30 2016-01-07 Dana-Farber Cancer Institute, Inc. Systems, apparatus, and methods related to magnetically-controlled three-dimensional tissue cultures

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3065148A (en) * 1959-07-14 1962-11-20 Technicon Instr Method and apparatus for use in conducting studies on cells
US3479141A (en) * 1967-05-17 1969-11-18 Technicon Corp Method and apparatus for analysis
US3694317A (en) * 1968-05-31 1972-09-26 Stanley Scher Method of and instrument for micro-biological analysis
US3741875A (en) * 1970-10-30 1973-06-26 Mount Sinai Res Foundation Inc Process and apparatus for obtaining a differential white blood cell count
US4116631A (en) * 1976-02-19 1978-09-26 Trinel Pierre Andre Method for microbiological analysis of liquid mediums
US4253846A (en) * 1979-11-21 1981-03-03 Technicon Instruments Corporation Method and apparatus for automated analysis of fluid samples
US4603108A (en) * 1979-05-02 1986-07-29 National Research Development Corp. Method for identification of bacterial species

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3065148A (en) * 1959-07-14 1962-11-20 Technicon Instr Method and apparatus for use in conducting studies on cells
US3479141A (en) * 1967-05-17 1969-11-18 Technicon Corp Method and apparatus for analysis
US3694317A (en) * 1968-05-31 1972-09-26 Stanley Scher Method of and instrument for micro-biological analysis
US3741875A (en) * 1970-10-30 1973-06-26 Mount Sinai Res Foundation Inc Process and apparatus for obtaining a differential white blood cell count
US4116631A (en) * 1976-02-19 1978-09-26 Trinel Pierre Andre Method for microbiological analysis of liquid mediums
US4603108A (en) * 1979-05-02 1986-07-29 National Research Development Corp. Method for identification of bacterial species
US4253846A (en) * 1979-11-21 1981-03-03 Technicon Instruments Corporation Method and apparatus for automated analysis of fluid samples

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CLINICAL CHEMISTRY, Volume 31, No. 9, issued September 1985, (Washington D.C.), M. CASSADAY, "Capsule Chemistry Technology for High-Speed Clinical Chemistry Analysis", see pages 1453-1456. *

Also Published As

Publication number Publication date
AU3182289A (en) 1989-09-22
CN1048062A (en) 1990-12-26
WO1989007880A2 (en) 1989-09-08

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