USRE34878E - Hypoglycemic agent - Google Patents

Hypoglycemic agent Download PDF

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USRE34878E
USRE34878E US08/157,564 US15756493A USRE34878E US RE34878 E USRE34878 E US RE34878E US 15756493 A US15756493 A US 15756493A US RE34878 E USRE34878 E US RE34878E
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phenylalanine
derivative
substituted
trans
methanol
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Shigeshi Toyoshima
Yoshiko Seto
Hisashi Shinkai
Koji Toi
Izumi Kamashiro
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Ajinomoto Co Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D213/78—Carbon atoms having three bonds to hetero atoms, with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C07D213/81—Amides; Imides
    • C07D213/82—Amides; Imides in position 3
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C233/00—Carboxylic acid amides
    • C07C233/57—Carboxylic acid amides having carbon atoms of carboxamide groups bound to carbon atoms of rings other than six-membered aromatic rings
    • C07C233/63—Carboxylic acid amides having carbon atoms of carboxamide groups bound to carbon atoms of rings other than six-membered aromatic rings having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C233/00—Carboxylic acid amides
    • C07C233/64—Carboxylic acid amides having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings
    • C07C233/81—Carboxylic acid amides having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups
    • C07C233/82—Carboxylic acid amides having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom
    • C07C233/87—Carboxylic acid amides having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom of a carbon skeleton containing six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C235/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms
    • C07C235/42—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton
    • C07C235/44—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring
    • C07C235/52—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring having the nitrogen atom of at least one of the carboxamide groups bound to an acyclic carbon atom of a hydrocarbon radical substituted by carboxyl groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
    • C07D307/77—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D307/78—Benzo [b] furans; Hydrogenated benzo [b] furans
    • C07D307/82—Benzo [b] furans; Hydrogenated benzo [b] furans with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the hetero ring
    • C07D307/84—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen
    • C07D307/85—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2601/00—Systems containing only non-condensed rings
    • C07C2601/06—Systems containing only non-condensed rings with a five-membered ring
    • C07C2601/08—Systems containing only non-condensed rings with a five-membered ring the ring being saturated
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2601/00—Systems containing only non-condensed rings
    • C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
    • C07C2601/14—The ring being saturated
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2601/00—Systems containing only non-condensed rings
    • C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
    • C07C2601/16—Systems containing only non-condensed rings with a six-membered ring the ring being unsaturated
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2602/00—Systems containing two condensed rings
    • C07C2602/02—Systems containing two condensed rings the rings having only two atoms in common
    • C07C2602/04—One of the condensed rings being a six-membered aromatic ring
    • C07C2602/08—One of the condensed rings being a six-membered aromatic ring the other ring being five-membered, e.g. indane
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2602/00—Systems containing two condensed rings
    • C07C2602/36—Systems containing two condensed rings the rings having more than two atoms in common
    • C07C2602/42—Systems containing two condensed rings the rings having more than two atoms in common the bicyclo ring system containing seven carbon atoms

Definitions

  • the present invention relates to hypoglycemic agents useful as antidiabetic drugs.
  • a D-phenylalanine derivative represented by the general formula: ##STR3## or a salt thereof, or a precursor which can be converted thereto in the human or animal body Such compounds can lower the value of blood sugar and thus can be used as an antidiabetic drug for an oral use as well as by injection.
  • R 1 is hydrogen, alkyl of 1 to 5 carbon atoms such as methyl, ethyl, n-propyl, isopropyl, n-butyl, and sec-butyl, aryl of 6 to 12 carbon atoms such as phenyl, tolyl, naphthyl, and ##STR5## aralkyl of 6 to 12 atoms such as benzyl, ##STR6## --CH 2 CO 2 R 3 , --CH(CH 3 )--OCO--R 3 , or --CH 2 --OCO--C(CH 3 ) 3 , R 2 is a group comprising aryl of 6 to 12 carbon atoms such as phenyl, naphthyl, and indanyl, a hetero six-membered ring such as quinolynyl, pyridyl, a hetero five-membered ring such as 2-benzofuranyl, cycloalkyl such as
  • substituents include a halogen atom such as fluorine or chlorine, a hydroxyl group, a C 1-5 alkyl group such as methyl, ethyl, trichloromethyl, trifluoromethyl, propyl, isopropyl, n-butyl, sec-butyl, and tert-butyl, a C 1-5 alkenyl group such as ethenyl, propenyl, and butenyl, an alkylidene group such as ##STR7## a C 1-5 alkyloxy such as methoxy and ethoxy, a C 1-5 alkyl group which has been substituted by such C 1-5 alkyloxy group such as methoxymethyl and 1-ethoxyethyl, a C 1-5 alkylene group which has been substituted by such C 1-5 alkyloxy group in the same manner as above such as 1-methoxyethylene.
  • a halogen atom such as fluorine or chlorine
  • R 1 stands for a hydrogen atom
  • it can be formed by conventional methods via the salts thereof with various cations such as an alkali metal, for example sodium and potassium, an alkali earth metal, for example, calcium, an inorganic base, for example, ammonia, an organic base, for example, cyclohexylamine, N-methyl-D-glucosamine, or a basic amino acid (lysine, arginine and the like).
  • the D-phenylalanine derivative as shown by the formula (I) mentioned above can be prepared by using conventional N-acylating reactions as in the Examples given below.
  • the D-phenylalanine derivatives used in the present invention are useful as a hypoglycemic agent for treating diabetic mammals including humans.
  • the derivatives can be used for lowering blood sugar by formulating them into a preparation such as tablets, capsules, and elixirs for oral administration and into an aseptic liquid preparation or an aseptic suspension preparation for parenteral administration such as subcutaneous, intramuscular, intavenous injection, and suppositories.
  • the D-phenylalamine derivatives in the present invention can be administered to a subject necessitating such treatment (animals and humans) in a dosage range of 0.1 to 1,000 mg per subject generally several times a day, that is, in a total daily dosage of 0.2 to 2,000 mg.
  • the dosages varies according to the seriousness of disease, the body weight of subjects, and other factors acknowledged by those skilled in the art.
  • D-phenylalanine derivatives as described above for the present invention, they may be converted to dosage forms such as tablets, granules, powders, capsules, injections and suppositories by conventional methods.
  • the D-phenylalanine derivative as the principal agent, adjuvants such as fillers, binders, disintegrators, lubricants, colors, and correctives, as necessary, and then formed by conventional methods into tablets, coated tablets, granules, powders, capsules and the like.
  • Examples of specific materials which can be incorporated into tablets, capsules, and so forth are as follows: fillers such as cornstarch, lactose, white sugar, glucose, sorbitol, and crystalline cellulose; binders such as polyvinyl alcohol, polyvinyl ether, ethyl cellulose, methyl cellulose, gum arabic, tragacanth gelatine, shellac, hydroxypropyl cellulose, hydroxypropyl starch, polyvinyl pyrrolidone; disintegrators such as starch, agar, gelatine powder, crystalline cellulose, calcium carbonate, sodium bicarbonate, calcium citrate, dextrin and pectin; lubricants such as magnesium stearate, talc, polyethylene glycol, silica, hardened plant oil; colors such as one which is allowed as an additive for the medicines; correctives such as cocoa powder, mentha herb, aromatic acid, mentha oil, borneol, cinnamon bark powder.
  • binders such as poly
  • the injectable formulations there may be added to the phenylalanine derivative as the principal agent, a pH adjusting agent, a buffer agent, a stabilizing agent, preservatives or the like, as necessary to produce a material for subcutaneous, intramuscular or intravenous injection by conventional methods.
  • D-Phenylalaine 2 g (12 mmole) was dissolved in 10% aqueous sodium hydroxide solution (10 ml), and acetone (10 ml) was added.
  • An acetone (5 ml) solution of 4-ethyl benzoyl chloride (2.5 g, 15 mmole) and a 10% aqueous sodium hydroxide solution were added dropwise to the mixture obtained above while stirring and cooling with ice over 20 minutes, the reaction solution being maintained at pH 10.
  • the reaction solution was returned to the room temperature, stirred for 3 hours, and made an acidic with a dilute hydrochloric acid solution to precipitate crystals.
  • the crystals were filtered, washed with water and recrystallized from ethyl acetate to obtain N-(4-ethylbenzoyl)-D-phenylalanine (3.0 g, yield 83%).
  • Cyclopentane carboxylic acid (1.5 g, 13 mmole) was dissolved in chloroform (50 ml), and N-hydroxysuccinimide 1.7 g was added.
  • N,N'-Dicyclohexylcarbodiimide (3.0 g) was gradually added to the mixture as obtained above while stirring and cooling with ice, and the mixture was stirred for 1 hour at the same temperature. The mixture was further stirred for 7 hours at room temperature. Glacial acetic acid (2 ml) was added to the mixture, and stirred for 1 hour. The insoluble matter was removed by filtration.
  • N-(4-isopropylcyclohexylcarbonyl)-D-phenylalanine was produced. It was crystallized from methanol-water to give the desired product (2.5 g, yield 61%).
  • ICR-CDI mice (Male, five weeks old, Body weight: 20 g) which had been bred for one week, were abstained from food for 18 hours, and then used as test subjects.
  • the phenylalanine derivative of the present invention was suspended in 0.5% CMC-0.05M tris-hydrochloride buffer (pH 7.4).
  • the sample solution thus obtained was administered orally in fixed amounts to the test subjects. A predetermined time later, the percentage decrease in blood glucose with the comparison to the control group was determined. The results are shown in the following table.
  • the filtrate was washed with saturated aqueous sodium bicarbonate (300 ml) and water (300 ml), and dried over magnesium sulfate.
  • the magnesium sulfate was removed by filtration, and the filtrate thus obtained was concentrated under reduced pressure to dryness.
  • the resultant substance was recrystallized from ethyl acetate to obtain cumic acid N-hydroxysuccinimide ester (18.8 g, yield 72 mmole).
  • the ester thus obtained above (18.8 g) was added to the chloroform solution (150 ml) of D-phenylalanine methyl ester hydrochloride (23.0 l g, 110 mmole) and triethylamine (10.8 g, 110 mmole), and the mixture thus obtained was stirred for 15 hours at room temperature.
  • the reaction solution was washed with 1N aqueous hydrochloric acid solution (300 ml), saturated aqueous sodium bicarbonate (300 ml) and water (300ml) and dried over magnesium sulfate.
  • the magnesium sulfate thus used was removed by filtration, and the filtrate thus obtained was concentrated under reduced pressure to dryness.
  • the methyl ester (9.0 g) thus obtained was dissolved in methanol (50 ml), and 1N aqueous sodium hydroxide solution (50 ml) was added thereto.
  • the mixture thus obtained was stirred for 10 minutes at room temperature and made acidic with an addition of a dilute aqueous hydrochloric acid solution to precipitate crystals.
  • the crystals were filtered, washed with water, and crystallized from methanol-water to give trans-4-isopropylcyclohexane carboxylic acid (6.8 g, yield 78%).
  • ICR-CDI mice (Male, five weeks old, Body weight: 20 g) were abstained from food for 18 hours, and then used as test subjects.
  • the phenylalanine derivative of the present invention was suspended in 0.5% CMC-0.14M sodium chloride buffer solution (pH 7.4).
  • the solution thus obtained was administered orally in fixed volume amounts to the test subjects. After a predetermined time, the percentage decrease of the blood glucose against the control group was determined. The results are shown in the following Table.
  • D-phenylalanine derivatives as described above can be used as an antidiabetic drug for oral administration as well as the more usual parenteral administration.

Abstract

A compound of D-phenylalanine derivative for hypoglycemic use, represented by the general formula ##STR1## R1 is selected from hydrogen, alkyl of 1 to 5 carbon atoms, aryl of 6 to 12 carbon atoms, aralkyl of 6 to 12 carbon atoms, ##STR2## --CH2 CO2 R3, --CH(CH3)--OCO--R3, and --CH2 --OCO--C(CH3)3 ; R2 is selected from groups comprising aryl of 6 to 12 carbon atoms, a hetero six-membered ring, a hetero five-membered ring, cycloalkyl, or cycloalkenyl, any of which groups may have one or more substituents; and R3 is selected from hydrogen and alkyl of 1 to 5 carbon atoms; the salts thereof, and precursors which can be converted thereto in the human or animal body.
Some of the compounds are novel per se.

Description

This application .Iadd.is a Reissue of Ser. No. 07/146,719, filed Feb. 21, 1988, U.S. Pat. No. 4,816,182, which is a .Iaddend.divisional of Ser. No. 844,970, filed Mar. 27, 1986, now abandoned.
FIELD OF THE INVENTION
The present invention relates to hypoglycemic agents useful as antidiabetic drugs.
BACKGROUND TO THE INVENTION
Hitherto, as antidiabetic drugs for oral use, there have been widely employed sulfonyl urea which shows hypoglycemic action particularly through a promotion of the secretion of insulin, and a biguanide which shows a hypoglycemic action particularly through the metabolism of sugar. However, they are somewhat unsatisfactory as to their side effects (see Textbook of Endocrinology 4th ed., 1968, p. 719 (Saunders); Diabetes, 19, 785, 1970; Ann. Rev. Pharmacol., 15, 351, 1975).
No report has ben found that a D-phenylalanine derivative possesses hypoglycemic action.
SUMMARY OF THE INVENTION AND DESCRIPTION OF PREFERRED EMBODIMENTS
According to one aspect of the invention there is provided for pharmaceutical, particularly hypoglycemic, use, a D-phenylalanine derivative represented by the general formula: ##STR3## or a salt thereof, or a precursor which can be converted thereto in the human or animal body. Such compounds can lower the value of blood sugar and thus can be used as an antidiabetic drug for an oral use as well as by injection.
Among the foregoing phenylalanine derivatives, those in the D-form represented by the general formula: ##STR4## and the salts thereof are novel.
In the above general formulae: R1 is hydrogen, alkyl of 1 to 5 carbon atoms such as methyl, ethyl, n-propyl, isopropyl, n-butyl, and sec-butyl, aryl of 6 to 12 carbon atoms such as phenyl, tolyl, naphthyl, and ##STR5## aralkyl of 6 to 12 atoms such as benzyl, ##STR6## --CH2 CO2 R3, --CH(CH3)--OCO--R3, or --CH2 --OCO--C(CH3)3, R2 is a group comprising aryl of 6 to 12 carbon atoms such as phenyl, naphthyl, and indanyl, a hetero six-membered ring such as quinolynyl, pyridyl, a hetero five-membered ring such as 2-benzofuranyl, cycloalkyl such as cyclohexyl and cyclopentyl, bicycloalkyl such as bicycloheptye, and cycloalkenyl such as 1-cyclohexenyl, 2-cyclohexeneyl, 3-cyclohexenyl, 1-cyclopentenyl and 2-cyclopentenyl, any of which groups optionally having one or more substituents; R3 is hydrogen or lower alkyl such as methyl, ethyl, isopropyl and pentyl; and R4 stands for those R2 groups which provide novel compounds hereof, notably phenyl which has one or more alkyl substituents of 2 to 5 carbon atoms, cyclohexyl which has one or more substituents cyclopentyl, bicycloalkyl, cycloalkenyl, indanyl or 2-benzofuranyl, any of which may have one or more substituents.
When an organic group in the above general formulae has a substituent, examples of such substituents include a halogen atom such as fluorine or chlorine, a hydroxyl group, a C1-5 alkyl group such as methyl, ethyl, trichloromethyl, trifluoromethyl, propyl, isopropyl, n-butyl, sec-butyl, and tert-butyl, a C1-5 alkenyl group such as ethenyl, propenyl, and butenyl, an alkylidene group such as ##STR7## a C1-5 alkyloxy such as methoxy and ethoxy, a C1-5 alkyl group which has been substituted by such C1-5 alkyloxy group such as methoxymethyl and 1-ethoxyethyl, a C1-5 alkylene group which has been substituted by such C1-5 alkyloxy group in the same manner as above such as 1-methoxyethylene. In the case of a substituted bicycloalkyl group as stated above, it can include a bicycloheptyl or a derivative thereof such as bicyclo (2,2,1)heptyl.
In the case of the compound represented by the general formula (I) wherein R1 stands for a hydrogen atom, it can be formed by conventional methods via the salts thereof with various cations such as an alkali metal, for example sodium and potassium, an alkali earth metal, for example, calcium, an inorganic base, for example, ammonia, an organic base, for example, cyclohexylamine, N-methyl-D-glucosamine, or a basic amino acid (lysine, arginine and the like).
The D-phenylalanine derivative as shown by the formula (I) mentioned above, can be prepared by using conventional N-acylating reactions as in the Examples given below.
Most of the phenylalanine derivatives supplied by this invention are novel compounds which have not been described yet in the literature.
The D-phenylalanine derivatives used in the present invention are useful as a hypoglycemic agent for treating diabetic mammals including humans. The derivatives can be used for lowering blood sugar by formulating them into a preparation such as tablets, capsules, and elixirs for oral administration and into an aseptic liquid preparation or an aseptic suspension preparation for parenteral administration such as subcutaneous, intramuscular, intavenous injection, and suppositories. The D-phenylalamine derivatives in the present invention can be administered to a subject necessitating such treatment (animals and humans) in a dosage range of 0.1 to 1,000 mg per subject generally several times a day, that is, in a total daily dosage of 0.2 to 2,000 mg. The dosages varies according to the seriousness of disease, the body weight of subjects, and other factors acknowledged by those skilled in the art.
To produce the preparations using the D-phenylalanine derivatives as described above for the present invention, they may be converted to dosage forms such as tablets, granules, powders, capsules, injections and suppositories by conventional methods.
For the production of oral preparations, there may be added the to the D-phenylalanine derivative as the principal agent, adjuvants such as fillers, binders, disintegrators, lubricants, colors, and correctives, as necessary, and then formed by conventional methods into tablets, coated tablets, granules, powders, capsules and the like.
Examples of specific materials which can be incorporated into tablets, capsules, and so forth are as follows: fillers such as cornstarch, lactose, white sugar, glucose, sorbitol, and crystalline cellulose; binders such as polyvinyl alcohol, polyvinyl ether, ethyl cellulose, methyl cellulose, gum arabic, tragacanth gelatine, shellac, hydroxypropyl cellulose, hydroxypropyl starch, polyvinyl pyrrolidone; disintegrators such as starch, agar, gelatine powder, crystalline cellulose, calcium carbonate, sodium bicarbonate, calcium citrate, dextrin and pectin; lubricants such as magnesium stearate, talc, polyethylene glycol, silica, hardened plant oil; colors such as one which is allowed as an additive for the medicines; correctives such as cocoa powder, mentha herb, aromatic acid, mentha oil, borneol, cinnamon bark powder. These tables and granules may be coated with sugar, gelatine, or the like, as desired.
For the production of the injectable formulations, there may be added to the phenylalanine derivative as the principal agent, a pH adjusting agent, a buffer agent, a stabilizing agent, preservatives or the like, as necessary to produce a material for subcutaneous, intramuscular or intravenous injection by conventional methods.
EXAMPLES
The present invention will be further explained in the following examples.
EXAMPLE 1 N-(4-Ethylbenzoyl)-D-phenylalanine
D-Phenylalaine 2 g (12 mmole) was dissolved in 10% aqueous sodium hydroxide solution (10 ml), and acetone (10 ml) was added. An acetone (5 ml) solution of 4-ethyl benzoyl chloride (2.5 g, 15 mmole) and a 10% aqueous sodium hydroxide solution were added dropwise to the mixture obtained above while stirring and cooling with ice over 20 minutes, the reaction solution being maintained at pH 10. The reaction solution was returned to the room temperature, stirred for 3 hours, and made an acidic with a dilute hydrochloric acid solution to precipitate crystals. The crystals were filtered, washed with water and recrystallized from ethyl acetate to obtain N-(4-ethylbenzoyl)-D-phenylalanine (3.0 g, yield 83%).
m.p. 165.5°-166° C. Specific Rotation [α]D 23 +4.4° (C=1, methanol).
EXAMPLES 2 TO 11
For Examples 2 to 8, in the same manner as in Example 1, using the following starting materials, each compound of 50 mmole, the following product compounds were produced. The compounds in Examples 9 to 11 were already known, and therefore were produced in accordance with the following literature references:
Example 9: J. Amer. Chem. Soc., 73, 1644, 1951,
Example 10: Pol. J. Chem., 53, 2239, 1979, and
Example 11: J. Chromatogr., 264, 63, 1983.
__________________________________________________________________________
Example                                                                   
     Starting          Yield                                              
                           M.P.  Specific                                 
No.  Material Product  (%) (°C.)                                   
                                 Rotation                                 
__________________________________________________________________________
2    D-phenylalanine                                                      
              N-(4-toldryl)-                                              
                       83  152-155                                        
                                 [α].sub.D .sup.28 +46.2°    
              D-phenylalanine    (C = 0.5, methanol)                      
3    D-phenylalanine                                                      
              N-(2-fluoro-                                                
                       74  91.5-93.5                                      
                                 [α].sub.D .sup.19 -8.8°     
              benzoyl)-D-        (C = 1, methanol)                        
              phenylalanine                                               
4    D-phenylalanine                                                      
              N-(3-fluoro-                                                
                       81  112.5-116                                      
                                 [α].sub.D .sup.22 +48.6°    
              benzoyl)-D-        (C = 1, methanol)                        
              phenylalanine                                               
5    D-phenylalanine                                                      
              N-(4-fluoro-                                                
                       80  142-145                                        
                                 [α].sub.D .sup.28 +40.4°    
              benzoyl)-D-        (C = 0.5, methanol)                      
              phenylalanine                                               
6    D-phenylalanine                                                      
              N-(3-trifluoro-                                             
                       77  118-119                                        
                                 [α].sub.D .sup.28 +40.4°    
              methylbenzoyl)-    (C + 1, methanol)                        
              D-phenylalanine                                             
7    D-phenylalanine                                                      
              N-(4-trifluoro-                                             
                       70    136-137.5                                    
                                 [α].sub.D .sup.28 +36.3°    
              methylbenzoyl)-    (C = 1, methanol)                        
              D-phenylalanine                                             
8    D-phenylalanine                                                      
              N-4-anisoyl)-D-                                             
                       65  85-90 [α].sub.D .sup.20 +60.2°    
              phenylalanine      (C = 0.5, methanol)                      
9    D-phenylalanine                                                      
              N-benzoyl-D-                                                
                       81                                                 
              phenylalanine                                               
10   D-phenylalanine                                                      
              N-nicotinoyl-                                               
                       62                                                 
              D-phenylalanine                                             
11   D-phenylalanine                                                      
              N-(2-naphthoyl)-                                            
                       83                                                 
              D-phenylalanine                                             
__________________________________________________________________________
EXAMPLE 12 N-Cyclopentylcarbonyl-D-phenylalanine
Cyclopentane carboxylic acid (1.5 g, 13 mmole) was dissolved in chloroform (50 ml), and N-hydroxysuccinimide 1.7 g was added. N,N'-Dicyclohexylcarbodiimide (3.0 g) was gradually added to the mixture as obtained above while stirring and cooling with ice, and the mixture was stirred for 1 hour at the same temperature. The mixture was further stirred for 7 hours at room temperature. Glacial acetic acid (2 ml) was added to the mixture, and stirred for 1 hour. The insoluble matter was removed by filtration. The filtrate was washed with saturated aqueous sodium bicarbonate solution (30 ml), 1N aqueous hydrochloric acid solution (30 ml), and water (30 ml), and dried over magnesium sulfate. The magnesium sulfate was removed by filtration, and the solution thus obtained was concentrated under reduced pressure to dryness. The matter was recrystallized from ethyl acetate to afford cyclopentane carboxylic acid N-hydroxysuccinimide ester (2.5 g, yield 91%).
The ester derivative thus obtained above (2.5 g), was dissolved in chloroform (20 ml). This solution was added to a chloroform solution (40 ml) of D-phenylalanine methyl ester hydrochloride (3.0 g, 14 mmole) and triethylamine (1.4 g), and the mixture thus obtained was stirred for 18 hours at room temperature. The reaction solution was washed with 1N aqueous hydrochloric acid solution (40 ml), saturated aqueous sodium bicarbonate solution (40 ml) and water (40 ml), and dried over magnesium sulfate. The magnesium sulfate was removed by filtration and the filtrate was concentrated under reduced pressure to dryness. The matter was recrystallized from ethyl acetate-n-hexane to afford N-cyclopentylcarbonyl-D-phenylalanine methyl ester (3.0 g, yield 84%).
The methyl ester derivative (3.0 g) thus obtained above, was dissolved in methanol (10 ml), and 1N aqueous sodium hydroxide solution (20 ml) was added. The mixture was stirred for 30 minutes at room temperature and then made acidic with an addition of a dilute hydrochloric acid to precipitate crystals. The crystals were filtered, washed with water, and recrystallized from methanol-water to give the desired product (2.7 g, yield 80%).
m.p. 108°-110° C. Specific Rotation [α]D 22 -35.2° (C=0.5, methanol).
EXAMPLES 13 TO 18
For Examples 13 to 16, in the same manner as in Example 12, using as the starting material the following compounds, each of 15 mmole, the following products were obtained. The compounds of Examples 17 and 18 stated in the above table were known, and therefore were produced in accordance with the following literature references:
Example 17: BEXXA BELG. NO. 893553, 48, 1981, and
Example 18: Bull. Chem. Soc. Jpn., 57, 2171, 1984.
__________________________________________________________________________
Example                                                                   
     Starting          Yield                                              
                           M.P. Specific Rotation                         
No.  Material                                                             
             Product   (%) (°C.)                                   
                                [α].sub.D .sup.22 (C = 0.5,         
__________________________________________________________________________
                                methanol                                  
13   2-benzofurane                                                        
             N-(2-benzofuran-                                             
                       59  114-116                                        
                                +89.6°                             
     carboxylic                                                           
             yl-carbonyl)-                                                
     acid    D-phenylalanine                                              
14   5-indane                                                             
             N-(5-indanyl-                                                
                       64  160-161                                        
                                +52.0°                             
     carboxylic                                                           
             carbonyl)-                                                   
     acid    D-phenylalanine                                              
15   3-cyclohexene                                                        
             N-(3-cyclo-                                                  
                       62  100-101                                        
                                -12.6°                             
     carboxylic                                                           
             hexenylcarbonyl-)                                            
     acid    D-phenylalanine                                              
16   bicyclo-                                                             
             N-(bicyclo-                                                  
                       50  179-181                                        
                                +33.4°                             
     [2,2,1]heptan-                                                       
             [2,2,1]heptan-2-                                             
     2-ylcarboxylic                                                       
             ylcarbonyl)-D-                                               
     acid    phenylalanine                                                
17   cyclohexene                                                          
             N-cyclohexyl-                                                
                       65                                                 
     carboxylic                                                           
             carbonyl-D-                                                  
     acid    phenylalanine                                                
18   benzoic acid                                                         
             N-benzoyl-D-                                                 
                       65                                                 
             phenylalanine                                                
             methyl ester                                                 
__________________________________________________________________________
EXAMPLE 19 N-(4-Isopropylcyclohexylcarbonyl)-D-phenylalanine
Platinum oxide (200 mg) as a catalyst was suspended in acetic acid (20 ml), and then (s)-(-)perillic acid (2 g, 12 mmole) was added. The mixture thus obtained was stirred for 8 hours at room temperature under a current of hydrogen gas. The catalyst was removed by filtration, and the filtrate was concentrated under reduced pressure to a dryness. The matter was recrystallized from methanol-water to obtain 4-isopropyl cyclohexane carboxylic acid (1.9 g, yield 93%).
After that, in the same manner as the reaction in Example 1, N-(4-isopropylcyclohexylcarbonyl)-D-phenylalanine was produced. It was crystallized from methanol-water to give the desired product (2.5 g, yield 61%).
m.p. 230°-232° C. Specific Rotation [α]D 22 -28.2° (C=0.5, methanol).
EXAMPLE 20
ICR-CDI mice (Male, five weeks old, Body weight: 20 g) which had been bred for one week, were abstained from food for 18 hours, and then used as test subjects.
The phenylalanine derivative of the present invention was suspended in 0.5% CMC-0.05M tris-hydrochloride buffer (pH 7.4). The sample solution thus obtained was administered orally in fixed amounts to the test subjects. A predetermined time later, the percentage decrease in blood glucose with the comparison to the control group was determined. The results are shown in the following table.
______________________________________                                    
Decrease in Blood Glucose (%)                                             
Example No. Amounts used (mg/kg)                                          
                            60 Minutes                                    
______________________________________                                    
1            25             34                                            
2           100             32                                            
3           100             24                                            
4           100             24                                            
5           100             43                                            
6           250             37                                            
7           100             33                                            
8           100             38                                            
9           100             34                                            
10          250             19                                            
11          250             17                                            
12           50             22                                            
13          100             31                                            
14          250             28                                            
15          100             28                                            
16          250             16                                            
17          100             27                                            
18          250             37                                            
19           25             50                                            
______________________________________
EXAMPLE 21 N-Cumoyl-D-Phenylalanine
Cumic acid (15.0 g, 91 mmole) was dissolved in chloroform (150 ml), and N-hydroxysuccinimide (11.4 g, 99 mmole) was added thereto. N,N'-Dicyclohexylcarbodiimide (20.4 g, 99 mmole) was added gradually to the mixture obtained above while cooling with ice and stirring, and then the mixture thus obtained was returned to room temperature. The mixture was further stirred for 15 hours at room temperature. Glacial acetic acid (5 ml) was added thereto, and the mixture thus obtained was stirred and the insoluble matter was removed by filtration. The filtrate was washed with saturated aqueous sodium bicarbonate (300 ml) and water (300 ml), and dried over magnesium sulfate. The magnesium sulfate was removed by filtration, and the filtrate thus obtained was concentrated under reduced pressure to dryness. The resultant substance was recrystallized from ethyl acetate to obtain cumic acid N-hydroxysuccinimide ester (18.8 g, yield 72 mmole).
The ester thus obtained above (18.8 g) was added to the chloroform solution (150 ml) of D-phenylalanine methyl ester hydrochloride (23.0 l g, 110 mmole) and triethylamine (10.8 g, 110 mmole), and the mixture thus obtained was stirred for 15 hours at room temperature. The reaction solution was washed with 1N aqueous hydrochloric acid solution (300 ml), saturated aqueous sodium bicarbonate (300 ml) and water (300ml) and dried over magnesium sulfate. The magnesium sulfate thus used was removed by filtration, and the filtrate thus obtained was concentrated under reduced pressure to dryness.
The residue thus obtained was recrystallized from ethyl acetate-n-hexane to obtain N-cumoyl-D-phenylalanine methyl ester (20.5 g, yield 69%).
The methyl ester thus obtained above (20.5 g) was dissolved in methanol (100 ml), and then 1N aqueous sodium hydroxide (100 ml) was added thereto. The mixture thus obtained was stirred for 10 minutes at room temperature, and was made acidic with an addition of diluted aqueous hydrochloric acid solution to precipitate crystals. The crystals were filtered, washed with water and recrystallized from methanol-water to give the desired product (18.1 g, yield 64%).
m.p. 177°-178° C. Specific Rotation [α]D 20 +25.5° (C=1, methanol).
EXAMPLES 22 to 30
In the same manner as in Example 21, using as the starting material the following compounds, each at 50 mmole, the following product compounds were produced.
__________________________________________________________________________
Example                                                                   
     Starting         Yield                                               
                          M.P.  Specific                                  
No.  Material                                                             
            Product   (%) (°C.)                                    
                                Rotation                                  
__________________________________________________________________________
                                [α].sub.D .sup.20 (C = 1,           
                                methanol)                                 
22   (s)-perillic                                                         
            N-[(s)-   44  109-110                                         
                                -37.2°                             
     acid   perilloyl]-D-                                                 
            phenylalinine                                                 
23   trans-4-n-                                                           
            N-(trans-4-n-                                                 
                      48  104-105                                         
                                -8.8°                              
     propylcyclo-                                                         
            propylcyclo-                                                  
     hexane hexylcarbonyl)-                                               
     carboxylic                                                           
            D-phenylalanine                                               
     acid                                                                 
24   trans-4-n-                                                           
            N-(trans-4-n-                                                 
                      50  144-145                                         
                                -7.5°                              
     butylcyclo-                                                          
            butylcyclohexyl-                                              
     hexane carbonyl)-D-                                                  
     carboxylic                                                           
            phenylalanine                                                 
     acid                                                                 
25   4-tert-butyl-                                                        
            N-(4-t-butyl-                                                 
                      55  177-178                                         
                                +51.5°                             
     benzoic acid                                                         
            benzoyl)-D-                                                   
            phenylalanine                                                 
26   cuminic acid                                                         
            N-cumoyl-L-                                                   
                      63  121-123                                         
                                [α].sub.D .sup.23 -29.3° (C  
                                = 1, methanol)                            
            phenylalanine                                                 
27   cyclopentane                                                         
            N-cyclopentyl-                                                
                      40  115-117                                         
                                [α].sub.D .sup.23 -30.1° (C  
                                = 1, methanol)                            
     carboxylic                                                           
            carbonyl-L-                                                   
     acid   phenylalanine                                                 
28   trans-4-                                                             
            N-(trans-4-                                                   
                      43  124-125                                         
                                [α].sub.D .sup.23 -11.5° (C  
                                = 1, methanol)                            
     methyl-cyclo-                                                        
            methylcyclohexyl-                                             
     hexane carbo-                                                        
            carbonyl)-D-phenyl-                                           
     xylic acid                                                           
            alanine                                                       
29   trans-4-ethyl-                                                       
            N-(trans-4-ethyl-                                             
                      53  96-97 [α].sub.D .sup.23 -11.1° (C  
                                = 1, methanol)                            
     cyclohexane                                                          
            cyclohexyl-carbo-                                             
     carboxylic                                                           
            nyl)-D-phenylala-                                             
     acid   nine                                                          
30   trans-4-t-                                                           
            N-(trans-4-t-                                                 
                      49  160-161                                         
                                [α].sub.D .sup.23 -9.0° (C = 
                                1, methanol)                              
     butyl-cyclo-                                                         
            butylcyclohexyl-                                              
     hexane carbo-                                                        
            carbonyl)-D-phenyl-                                           
     xylic acid                                                           
            alanine                                                       
__________________________________________________________________________
EXAMPLE 31 N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine
Platinum oxide (500 mg) as a catalyst was suspended in acetic acid (50 ml) and cumic acid (10 g, 61 mmole) was added thereto. The mixture thus obtained was stirred vigorously for 2 hours at room temperature under a pressure of hydrogen 5 kg/cm2. The catalyst was removed by filtration, and the filtrate was concentrated under reduced pressure to a solid state. The resultant substance was distilled under reduced pressure of 1 mmHg (1.3×10-3 kg/cm2), at 113°-116° C. to obtain 4-isopropylcyclohexane carboxylic acid yielding 10 g (96%) in a ratio of 3 parts of cis-form per 1 part of trans-form by weight.
To methanol (70 ml) at less than -20° C., thionyl chloride (17 ml) was added dropwise, and the carboxylic acid (10 g) as obtained above was added. The mixture thus obtained was stirred for 15 hours at room temperature, and then concentrated under reduced pressure to a solid substance. The substance thus obtained was distilled under reduced pressure of 0.7 mmHg (9.2×10-4 kg/cm2) at 66° C. to obtain 4-isopropylcyclohexane carboxylic acid methyl ester (9.5 g, yield 88%).
To the methyl ester (b 9.5 g) thus obtained, sodium hydride (120 mg) was added, and the mixture was heated at 150° C. for 2 hours under a current of nitrogen gas. The reaction solution was cooled and then subjected to a reduced pressure distillation of 0.7 mmHG (9.2×10-4 kg/cm2) at 66° C. to obtain 4-isopropylcyclohexane carboxylic acid methyl ester in a ratio of 6 parts trans-form per 1 part cis-form.
The methyl ester (9.0 g) thus obtained was dissolved in methanol (50 ml), and 1N aqueous sodium hydroxide solution (50 ml) was added thereto. The mixture thus obtained was stirred for 10 minutes at room temperature and made acidic with an addition of a dilute aqueous hydrochloric acid solution to precipitate crystals. The crystals were filtered, washed with water, and crystallized from methanol-water to give trans-4-isopropylcyclohexane carboxylic acid (6.8 g, yield 78%).
After that, in the same manner as in Example 21, using as a starting material the carboxylic acid derivative (6.8 g, 40 mmole), N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine was produced, and recrystallized from methanol-water to give the desired product (8.2 g, yield 65%).
m.p. 129°-130° C. Specific Rotation [α]D 20 -9.4° (C=1, methanol).
EXAMPLES 32 TO 35
In the same manner as in Example 26, using as the starting material the following compounds, each of 40 mmole, the following product compounds were produced.
__________________________________________________________________________
Example                                                                   
     Starting         Yield                                               
                          M.P.   Specific Rotation                        
No.  Material                                                             
            Product   (%) (°C.)                                    
                                 [α].sub.D .sup.20 (C = 1,          
__________________________________________________________________________
                                 methanol)                                
32   trans-4-                                                             
            4-(trans-4-                                                   
                      52  137-138                                         
                                 +8.8°                             
     isopropyl-                                                           
            isopropylcyclo-                                               
     cyclohexane                                                          
            hexylcarbonyl)-D-                                             
     carboxylic                                                           
            phenylalanine                                                 
     acid   methyl ester                                                  
33   trans-4-                                                             
            N-(trans-4-                                                   
                      56  130-131                                         
                                 +9.5°                             
     isopropyl-                                                           
            isopropylcyclo-                                               
     cyolohexane                                                          
            hexylcarbonyl)-                                               
     carboxylic                                                           
            L-phenylalanine                                               
     acid                                                                 
34   trans-4-                                                             
            N-(trans-4-                                                   
                      66  134--135                                        
                                 --                                       
     isopropyl-                                                           
            isopropylcyclo-                                               
     cyclohexane                                                          
            hexylcarbonyl)-                                               
     carboxylic                                                           
            2-phenylethylamine                                            
     acid                                                                 
35   trans-4-                                                             
            N-(trans-4-                                                   
                      58    129--130.5                                    
                                 +8.4°                             
     isopropyl-                                                           
            isopropylcyclo-                                               
     cyclohexane                                                          
            hexylcarbonyl)-                                               
     carboxylic                                                           
            D-phenylalanine                                               
     acid   benzylester                                                   
__________________________________________________________________________
EXAMPLE 26 N-(Cis-4-isopropylcyclohexylcarbonyl)-D-phenylalanine
4-Isopropylcyclohexane carboxylic acid methyl ester (9.5 g) (Cis-form:trans-form=3:1) was obtained in the same manner as in Example 26. Cis-4-isopropylcyclohexane carboxylic acid methyl ester (7 g) was obtained from the product thus obtained by a high performance liquid chromatography of YMC A-043 column using as the solvent a mixture of n-hexane and 1,2-dichloro ethane in a ratio of 75:25.
After that, in the same manner as in Example 21, using as a starting material the cis-form thus obtained (6.5 g, 38 mmole), N-(Cis-4-isopropylcyclohexylcarbonyl)-D-phenylalanine was produced, and recrystallized from methanol-water to give the desired product (8 g, yield 66%).
m.p. 111°-112° C. Specific Rotation [α]D 20 -13.2° (C=1, methanol).
EXAMPLE 37
ICR-CDI mice (Male, five weeks old, Body weight: 20 g) were abstained from food for 18 hours, and then used as test subjects.
The phenylalanine derivative of the present invention was suspended in 0.5% CMC-0.14M sodium chloride buffer solution (pH 7.4). The solution thus obtained was administered orally in fixed volume amounts to the test subjects. After a predetermined time, the percentage decrease of the blood glucose against the control group was determined. The results are shown in the following Table.
______________________________________                                    
Example                                                                   
       Amounts used in sample                                             
                        Decrease in blood glucose                         
No.    mg/kg body weight                                                  
                        after 60 minutes (%)                              
______________________________________                                    
21     25               26                                                
22     100              43                                                
23     100              35                                                
24     100              30                                                
25     100              32                                                
26     100               0                                                
27     100               0                                                
28     6.25             24                                                
29     6.25             31                                                
30     6.25             30                                                
31     1.5              30                                                
32     6.25             37                                                
33     100              23                                                
34     100              14                                                
35     25               24                                                
36     100              27                                                
______________________________________
It is clear from the foregoing that the D-phenylalanine derivatives as described above can be used as an antidiabetic drug for oral administration as well as the more usual parenteral administration.

Claims (15)

We claim:
1. A D-phenylalanine derivative of the formula
R.sup.4 --CO--NR.sup.3 --CH(COOR.sup.1)--CH.sub.2 --C.sub.6 H.sub.5
or a salt thereof or a precursor which can be converted into said D-phenylalanine derivative in vivo, wherein:
R1 is hydrogen or C1-5 alkyl,
R.sup. is hydrogen or C1-5 alkyl; and
R4 is cyclohexane substituted at the 4- or 5-position by methyl, ethyl, ispropyl, tert-butyl, ethene, or isopropene or cyclohexene substituted at the 4- or 5-position by methyl, ethyl, isopropyl, tert-butyl, ethene, or isopropene.
2. The D-phenylalanine derivative of claim 1, wherein R4 is said substituted cyclohexane.
3. The D-phenylalanine derivative of claim 1, wherein R4 is said substituted cyclohexane.
4. The D-phenylalanine derivative of claim 1, wherein the said derivative is N-(4-isopropylcyclohexylcarbonyl)-D-phenylalanine.
5. The D-phenylalanine derivative of claim 1, wherein the said derivative is N-(4-isopropylcyclohexylcarbonyl)-D-phenylalanine: N-[(S)-perilloyl]-D-phenylalanine; N-(4-methylcyclohexylcarbonyl)-D-phenylalanine; N-(4-ethylcyclohexylcarbonyl)-D-phenylalanine; or N-(4-t-butylcyclohexylcarbonyl)-D-phenylalanine.
6. The D-phenylalanine derivative of claim 1, wherein the said derivative is N-[(s)-perilloyl]-D-phenylalanine; N-(trans-4-methylcyclohexylcarbonyl)-D-phenylalanine; N-(trans-4-ethylcyclohexylcarbonyl)-D-phenylalanine; N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine; or N-(trans-4-t-butylcyclohexylcarbonyl)-D-phenylalanine.
7. The D-phenylalanine derivative of claim 1, wherein R1 is hydrogen and R3 is hydrogen.
8. The D-phenylalanine derivative of claim 1, wherein R4 is perilloyl.
9. The D-phenylalanine derivative of claim 1, wherein said substituted cyclohexane is substituted at the 4-position.
10. The D-phenylalanine derivative of claim 1, wherein said substituted cyclohexane is substituted at the 5-position.
11. The D-phenylalanine derivative of claim 1, wherein said substituted cyclohexene is substituted at the 4-position.
12. The D-phenylalanine derivative of claim 1, wherein said substituted cyclohexene is substituted at the 5-position.
13. The D-phenylalanine derivative of claim 1, wherein said substituted cyclohexane or said substituted cyclohexene is substituted with methyl, ethyl, isopropyl or tert-butyl.
14. The D-phenylalanine derivative of claim 1, wherein said substituted cyclohexane or said substituted cyclohexene is substituted by ethene, or isopropene.
15. A pharmaceutical composition, comprising a D-phenylalanine derivative of claim 1 and a pharmaceutical excipient. .Iadd.16. The compound N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine. .Iaddend.
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