US7972859B2 - Authentication of ingestible products using saccharides as markers - Google Patents
Authentication of ingestible products using saccharides as markers Download PDFInfo
- Publication number
- US7972859B2 US7972859B2 US11/768,766 US76876607A US7972859B2 US 7972859 B2 US7972859 B2 US 7972859B2 US 76876607 A US76876607 A US 76876607A US 7972859 B2 US7972859 B2 US 7972859B2
- Authority
- US
- United States
- Prior art keywords
- saccharide
- binding protein
- testing
- product
- ingestible product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active, expires
Links
- 150000001720 carbohydrates Chemical class 0.000 title claims abstract description 147
- 238000012360 testing method Methods 0.000 claims abstract description 76
- 102000014914 Carrier Proteins Human genes 0.000 claims abstract description 46
- 108091008324 binding proteins Proteins 0.000 claims abstract description 44
- 238000000034 method Methods 0.000 claims abstract description 32
- 230000009870 specific binding Effects 0.000 claims abstract description 28
- 230000008859 change Effects 0.000 claims abstract description 12
- 235000013305 food Nutrition 0.000 claims abstract description 10
- 235000013361 beverage Nutrition 0.000 claims abstract description 9
- 239000003550 marker Substances 0.000 claims description 64
- 230000027455 binding Effects 0.000 claims description 37
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 23
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 23
- 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 claims description 20
- 238000004519 manufacturing process Methods 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- DFYRUELUNQRZTB-UHFFFAOYSA-N apocynin Chemical compound COC1=CC(C(C)=O)=CC=C1O DFYRUELUNQRZTB-UHFFFAOYSA-N 0.000 claims description 6
- JMFRWRFFLBVWSI-NSCUHMNNSA-N coniferol Chemical compound COC1=CC(\C=C\CO)=CC=C1O JMFRWRFFLBVWSI-NSCUHMNNSA-N 0.000 claims description 6
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 6
- 239000000654 additive Substances 0.000 claims description 5
- 230000000996 additive effect Effects 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- CGSJERNEFDBIGH-UHFFFAOYSA-N 1-(4-hydroxy-3-methoxyphenyl)but-3-en-2-one Chemical compound COC1=CC(CC(=O)C=C)=CC=C1O CGSJERNEFDBIGH-UHFFFAOYSA-N 0.000 claims description 3
- GQBVUGXINLMDTO-UHFFFAOYSA-N 2-[(4-hydroxy-3-methoxyphenyl)methylidene]butanoic acid Chemical compound CCC(C(O)=O)=CC1=CC=C(O)C(OC)=C1 GQBVUGXINLMDTO-UHFFFAOYSA-N 0.000 claims description 3
- NXTDJHZGHOFSQG-UHFFFAOYSA-N 3-phenoxybenzoic acid Chemical compound OC(=O)C1=CC=CC(OC=2C=CC=CC=2)=C1 NXTDJHZGHOFSQG-UHFFFAOYSA-N 0.000 claims description 3
- VDBJCDWTNCKRTF-UHFFFAOYSA-N 6'-hydroxyspiro[2-benzofuran-3,9'-9ah-xanthene]-1,3'-dione Chemical compound O1C(=O)C2=CC=CC=C2C21C1C=CC(=O)C=C1OC1=CC(O)=CC=C21 VDBJCDWTNCKRTF-UHFFFAOYSA-N 0.000 claims description 3
- 240000001592 Amaranthus caudatus Species 0.000 claims description 3
- 235000009328 Amaranthus caudatus Nutrition 0.000 claims description 3
- KMNTUASVUKNVJS-UHFFFAOYSA-N Ponceau S (acid form) Chemical compound OC1=C(S(O)(=O)=O)C=C2C=C(S(O)(=O)=O)C=CC2=C1N=NC(C(=C1)S(O)(=O)=O)=CC=C1N=NC1=CC=C(S(O)(=O)=O)C=C1 KMNTUASVUKNVJS-UHFFFAOYSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 239000004178 amaranth Substances 0.000 claims description 3
- 235000012735 amaranth Nutrition 0.000 claims description 3
- 239000003086 colorant Substances 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 229940119526 coniferyl alcohol Drugs 0.000 claims description 3
- GLNDAGDHSLMOKX-UHFFFAOYSA-N coumarin 120 Chemical compound C1=C(N)C=CC2=C1OC(=O)C=C2C GLNDAGDHSLMOKX-UHFFFAOYSA-N 0.000 claims description 3
- 239000004148 curcumin Substances 0.000 claims description 3
- 235000012754 curcumin Nutrition 0.000 claims description 3
- 229940109262 curcumin Drugs 0.000 claims description 3
- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 claims description 3
- YSVBPNGJESBVRM-UHFFFAOYSA-L disodium;4-[(1-oxido-4-sulfonaphthalen-2-yl)diazenyl]naphthalene-1-sulfonate Chemical compound [Na+].[Na+].C1=CC=C2C(N=NC3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)O)=CC=C(S([O-])(=O)=O)C2=C1 YSVBPNGJESBVRM-UHFFFAOYSA-L 0.000 claims description 3
- IINNWAYUJNWZRM-UHFFFAOYSA-L erythrosin B Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(I)C(=O)C(I)=C2OC2=C(I)C([O-])=C(I)C=C21 IINNWAYUJNWZRM-UHFFFAOYSA-L 0.000 claims description 3
- 239000004174 erythrosine Substances 0.000 claims description 3
- 235000012732 erythrosine Nutrition 0.000 claims description 3
- 229940011411 erythrosine Drugs 0.000 claims description 3
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 claims description 3
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 claims description 3
- XMZOLZUIEQZXOB-UHFFFAOYSA-N hexyl 4-hydroxy-3-methoxybenzoate Chemical compound CCCCCCOC(=O)C1=CC=C(O)C(OC)=C1 XMZOLZUIEQZXOB-UHFFFAOYSA-N 0.000 claims description 3
- NRZRRZAVMCAKEP-UHFFFAOYSA-N naphthionic acid Chemical compound C1=CC=C2C(N)=CC=C(S(O)(=O)=O)C2=C1 NRZRRZAVMCAKEP-UHFFFAOYSA-N 0.000 claims description 3
- 239000004175 ponceau 4R Substances 0.000 claims description 3
- 235000012731 ponceau 4R Nutrition 0.000 claims description 3
- SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical compound [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 claims description 3
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 claims description 3
- 235000012141 vanillin Nutrition 0.000 claims description 3
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 claims description 3
- 239000011782 vitamin Substances 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 235000013343 vitamin Nutrition 0.000 claims description 3
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 2
- 125000003071 maltose group Chemical group 0.000 claims 1
- 239000000047 product Substances 0.000 description 74
- 108010038196 saccharide-binding proteins Proteins 0.000 description 27
- 102000004169 proteins and genes Human genes 0.000 description 19
- 108090000623 proteins and genes Proteins 0.000 description 19
- 229920000856 Amylose Polymers 0.000 description 11
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 10
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 10
- 239000000356 contaminant Substances 0.000 description 10
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 9
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 9
- 238000001514 detection method Methods 0.000 description 9
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 8
- 235000000346 sugar Nutrition 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 108010089814 Plant Lectins Proteins 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 229930182830 galactose Natural products 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 239000003726 plant lectin Substances 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 5
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 5
- 229920002684 Sepharose Polymers 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 5
- 229910052799 carbon Inorganic materials 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 150000002016 disaccharides Chemical class 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 239000011347 resin Substances 0.000 description 5
- 229920005989 resin Polymers 0.000 description 5
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 4
- WQZGKKKJIJFFOK-IVMDWMLBSA-N D-allopyranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@H](O)[C@@H]1O WQZGKKKJIJFFOK-IVMDWMLBSA-N 0.000 description 4
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 4
- ZAQJHHRNXZUBTE-UHFFFAOYSA-N D-threo-2-Pentulose Natural products OCC(O)C(O)C(=O)CO ZAQJHHRNXZUBTE-UHFFFAOYSA-N 0.000 description 4
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 4
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 4
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 4
- 239000000020 Nitrocellulose Substances 0.000 description 4
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 239000002250 absorbent Substances 0.000 description 4
- 230000002745 absorbent Effects 0.000 description 4
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 4
- 239000011324 bead Substances 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 239000007795 chemical reaction product Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000008101 lactose Substances 0.000 description 4
- 239000004816 latex Substances 0.000 description 4
- 229920000126 latex Polymers 0.000 description 4
- 150000002772 monosaccharides Chemical class 0.000 description 4
- 238000002703 mutagenesis Methods 0.000 description 4
- 231100000350 mutagenesis Toxicity 0.000 description 4
- 229920001220 nitrocellulos Polymers 0.000 description 4
- -1 oligomaltose Chemical compound 0.000 description 4
- 229920001542 oligosaccharide Polymers 0.000 description 4
- 150000002482 oligosaccharides Chemical class 0.000 description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 3
- DBTMGCOVALSLOR-UHFFFAOYSA-N 32-alpha-galactosyl-3-alpha-galactosyl-galactose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(OC2C(C(CO)OC(O)C2O)O)OC(CO)C1O DBTMGCOVALSLOR-UHFFFAOYSA-N 0.000 description 3
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 3
- RXVWSYJTUUKTEA-UHFFFAOYSA-N D-maltotriose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 RXVWSYJTUUKTEA-UHFFFAOYSA-N 0.000 description 3
- ZAQJHHRNXZUBTE-WUJLRWPWSA-N D-xylulose Chemical compound OC[C@@H](O)[C@H](O)C(=O)CO ZAQJHHRNXZUBTE-WUJLRWPWSA-N 0.000 description 3
- 229930091371 Fructose Natural products 0.000 description 3
- 239000005715 Fructose Substances 0.000 description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 3
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 3
- 108010046569 Galectins Proteins 0.000 description 3
- 102000007563 Galectins Human genes 0.000 description 3
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 229950006780 n-acetylglucosamine Drugs 0.000 description 3
- 239000000825 pharmaceutical preparation Substances 0.000 description 3
- 229940127557 pharmaceutical product Drugs 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 150000004804 polysaccharides Chemical class 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 3
- 102000005962 receptors Human genes 0.000 description 3
- 108020003175 receptors Proteins 0.000 description 3
- 229920006395 saturated elastomer Polymers 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 235000015096 spirit Nutrition 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 3
- KFEUJDWYNGMDBV-UHFFFAOYSA-N (N-Acetyl)-glucosamin-4-beta-galaktosid Natural products OC1C(NC(=O)C)C(O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 KFEUJDWYNGMDBV-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 108090000342 C-Type Lectins Proteins 0.000 description 2
- 102000003930 C-Type Lectins Human genes 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 108010078791 Carrier Proteins Proteins 0.000 description 2
- 102000012286 Chitinases Human genes 0.000 description 2
- 108010022172 Chitinases Proteins 0.000 description 2
- YTBSYETUWUMLBZ-UHFFFAOYSA-N D-Erythrose Natural products OCC(O)C(O)C=O YTBSYETUWUMLBZ-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-CBPJZXOFSA-N D-Gulose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O WQZGKKKJIJFFOK-CBPJZXOFSA-N 0.000 description 2
- WQZGKKKJIJFFOK-WHZQZERISA-N D-aldose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-WHZQZERISA-N 0.000 description 2
- LKDRXBCSQODPBY-JDJSBBGDSA-N D-allulose Chemical compound OCC1(O)OC[C@@H](O)[C@@H](O)[C@H]1O LKDRXBCSQODPBY-JDJSBBGDSA-N 0.000 description 2
- YTBSYETUWUMLBZ-IUYQGCFVSA-N D-erythrose Chemical compound OC[C@@H](O)[C@@H](O)C=O YTBSYETUWUMLBZ-IUYQGCFVSA-N 0.000 description 2
- ZAQJHHRNXZUBTE-NQXXGFSBSA-N D-ribulose Chemical compound OC[C@@H](O)[C@@H](O)C(=O)CO ZAQJHHRNXZUBTE-NQXXGFSBSA-N 0.000 description 2
- YTBSYETUWUMLBZ-QWWZWVQMSA-N D-threose Chemical compound OC[C@@H](O)[C@H](O)C=O YTBSYETUWUMLBZ-QWWZWVQMSA-N 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 206010056474 Erythrosis Diseases 0.000 description 2
- 102000000795 Galectin 1 Human genes 0.000 description 2
- 108010001498 Galectin 1 Proteins 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 description 2
- WQZGKKKJIJFFOK-VSOAQEOCSA-N L-altropyranose Chemical compound OC[C@@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-VSOAQEOCSA-N 0.000 description 2
- ZAQJHHRNXZUBTE-WVZVXSGGSA-N L-xylulose Chemical compound OC[C@H](O)[C@@H](O)C(=O)CO ZAQJHHRNXZUBTE-WVZVXSGGSA-N 0.000 description 2
- 102000004856 Lectins Human genes 0.000 description 2
- 108090001090 Lectins Proteins 0.000 description 2
- 229920000057 Mannan Polymers 0.000 description 2
- OVRNDRQMDRJTHS-CBQIKETKSA-N N-Acetyl-D-Galactosamine Chemical compound CC(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@H](O)[C@@H]1O OVRNDRQMDRJTHS-CBQIKETKSA-N 0.000 description 2
- MBLBDJOUHNCFQT-UHFFFAOYSA-N N-acetyl-D-galactosamine Natural products CC(=O)NC(C=O)C(O)C(O)C(O)CO MBLBDJOUHNCFQT-UHFFFAOYSA-N 0.000 description 2
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 2
- KFEUJDWYNGMDBV-LODBTCKLSA-N N-acetyllactosamine Chemical compound O[C@@H]1[C@@H](NC(=O)C)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 KFEUJDWYNGMDBV-LODBTCKLSA-N 0.000 description 2
- HESSGHHCXGBPAJ-UHFFFAOYSA-N N-acetyllactosamine Natural products CC(=O)NC(C=O)C(O)C(C(O)CO)OC1OC(CO)C(O)C(O)C1O HESSGHHCXGBPAJ-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 2
- 101100215694 Rhizobium meliloti (strain 1021) agpA gene Proteins 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-STGXQOJASA-N alpha-D-lyxopyranose Chemical compound O[C@@H]1CO[C@H](O)[C@@H](O)[C@H]1O SRBFZHDQGSBBOR-STGXQOJASA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000010256 biochemical assay Methods 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- UQPHVQVXLPRNCX-UHFFFAOYSA-N erythrulose Chemical compound OCC(O)C(=O)CO UQPHVQVXLPRNCX-UHFFFAOYSA-N 0.000 description 2
- 238000002523 gelfiltration Methods 0.000 description 2
- 150000002453 idose derivatives Chemical class 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- BJHIKXHVCXFQLS-PQLUHFTBSA-N keto-D-tagatose Chemical compound OC[C@@H](O)[C@H](O)[C@H](O)C(=O)CO BJHIKXHVCXFQLS-PQLUHFTBSA-N 0.000 description 2
- 239000002523 lectin Substances 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 235000013522 vodka Nutrition 0.000 description 2
- DKXNBNKWCZZMJT-ZYAMEUKBSA-N (2r,3r,4s,5r)-2,3,5,6-tetrahydroxy-4-[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhexanal Chemical compound O=C[C@H](O)[C@@H](O)[C@H]([C@H](O)CO)O[C@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O DKXNBNKWCZZMJT-ZYAMEUKBSA-N 0.000 description 1
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- ZUQUTHURQVDNKF-WZPXOXCRSA-N 1-[(3S,4R,5S,6R)-3-amino-2,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]ethanone Chemical compound C(C)(=O)C1(O)[C@@H](N)[C@@H](O)[C@H](O)[C@H](O1)CO ZUQUTHURQVDNKF-WZPXOXCRSA-N 0.000 description 1
- AYRXSINWFIIFAE-UHFFFAOYSA-N 2,3,4,5-tetrahydroxy-6-[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhexanal Chemical compound OCC1OC(OCC(O)C(O)C(O)C(O)C=O)C(O)C(O)C1O AYRXSINWFIIFAE-UHFFFAOYSA-N 0.000 description 1
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 1
- KZMAWJRXKGLWGS-UHFFFAOYSA-N 2-chloro-n-[4-(4-methoxyphenyl)-1,3-thiazol-2-yl]-n-(3-methoxypropyl)acetamide Chemical compound S1C(N(C(=O)CCl)CCCOC)=NC(C=2C=CC(OC)=CC=2)=C1 KZMAWJRXKGLWGS-UHFFFAOYSA-N 0.000 description 1
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- MIDXCONKKJTLDX-UHFFFAOYSA-N 3,5-dimethylcyclopentane-1,2-dione Chemical compound CC1CC(C)C(=O)C1=O MIDXCONKKJTLDX-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 108090000672 Annexin A5 Proteins 0.000 description 1
- 102000004121 Annexin A5 Human genes 0.000 description 1
- 102000005427 Asialoglycoprotein Receptor Human genes 0.000 description 1
- 241000006382 Bacillus halodurans Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 102000000844 Cell Surface Receptors Human genes 0.000 description 1
- 241000186320 Cellulomonas fimi Species 0.000 description 1
- 241001532572 Cellvibrio mixtus Species 0.000 description 1
- 244000237791 Chionanthus virginicus Species 0.000 description 1
- 235000015256 Chionanthus virginicus Nutrition 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 102100037328 Chitotriosidase-1 Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 101001042481 Cricetulus longicaudatus Galectin-3 Proteins 0.000 description 1
- HMFHBZSHGGEWLO-IOVATXLUSA-N D-xylofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@H]1O HMFHBZSHGGEWLO-IOVATXLUSA-N 0.000 description 1
- 108010016626 Dipeptides Proteins 0.000 description 1
- 241001634308 Dolichus Species 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 108010024212 E-Selectin Proteins 0.000 description 1
- 102100023471 E-selectin Human genes 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001198387 Escherichia coli BL21(DE3) Species 0.000 description 1
- 102000000802 Galectin 3 Human genes 0.000 description 1
- 101710180893 Gliolectin Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101100189357 Homo sapiens PAPOLG gene Proteins 0.000 description 1
- SHZGCJCMOBCMKK-PQMKYFCFSA-N L-Fucose Natural products C[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O SHZGCJCMOBCMKK-PQMKYFCFSA-N 0.000 description 1
- 102100029137 L-xylulose reductase Human genes 0.000 description 1
- 108010080643 L-xylulose reductase Proteins 0.000 description 1
- 101710142063 Lactose-binding protein Proteins 0.000 description 1
- 235000019687 Lamb Nutrition 0.000 description 1
- DUKURNFHYQXCJG-UHFFFAOYSA-N Lewis A pentasaccharide Natural products OC1C(O)C(O)C(C)OC1OC1C(OC2C(C(O)C(O)C(CO)O2)O)C(NC(C)=O)C(OC2C(C(OC3C(OC(O)C(O)C3O)CO)OC(CO)C2O)O)OC1CO DUKURNFHYQXCJG-UHFFFAOYSA-N 0.000 description 1
- 241001521394 Maackia amurensis Species 0.000 description 1
- 101710144262 Maltotriose-binding protein Proteins 0.000 description 1
- 102000009112 Mannose-Binding Lectin Human genes 0.000 description 1
- 108010087870 Mannose-Binding Lectin Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101710135898 Myc proto-oncogene protein Proteins 0.000 description 1
- 102100038895 Myc proto-oncogene protein Human genes 0.000 description 1
- OVRNDRQMDRJTHS-RTRLPJTCSA-N N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-RTRLPJTCSA-N 0.000 description 1
- SQVRNKJHWKZAKO-LUWBGTNYSA-N N-acetylneuraminic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)CC(O)(C(O)=O)O[C@H]1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-LUWBGTNYSA-N 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 102100040153 Poly(A) polymerase gamma Human genes 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 238000001069 Raman spectroscopy Methods 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 101710105454 Rhamnose-binding lectin Proteins 0.000 description 1
- 241000702670 Rotavirus Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 108010017898 Shiga Toxins Proteins 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 241000589196 Sinorhizobium meliloti Species 0.000 description 1
- UQZIYBXSHAGNOE-USOSMYMVSA-N Stachyose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 UQZIYBXSHAGNOE-USOSMYMVSA-N 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- 101710198996 Sucrose-binding protein Proteins 0.000 description 1
- 241000186339 Thermoanaerobacter Species 0.000 description 1
- 101710150448 Transcriptional regulator Myc Proteins 0.000 description 1
- FLUADVWHMHPUCG-OVEXVZGPSA-N Verbascose Natural products O(C[C@H]1[C@H](O)[C@H](O)[C@H](O)[C@@H](OC[C@@H]2[C@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]3(CO)[C@H](O)[C@@H](O)[C@@H](CO)O3)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 FLUADVWHMHPUCG-OVEXVZGPSA-N 0.000 description 1
- 102100025455 Vesicular integral-membrane protein VIP36 Human genes 0.000 description 1
- 101710144407 Vesicular integral-membrane protein VIP36 Proteins 0.000 description 1
- LFFQNKFIEIYIKL-UHFFFAOYSA-N Xylopentaose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(OC4C(C(O)C(O)OC4)O)OC3)O)OC2)O)OC1 LFFQNKFIEIYIKL-UHFFFAOYSA-N 0.000 description 1
- 241001147774 [Eubacterium] cellulosolvens Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 235000013334 alcoholic beverage Nutrition 0.000 description 1
- GZCGUPFRVQAUEE-KVTDHHQDSA-N aldehydo-D-mannose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O GZCGUPFRVQAUEE-KVTDHHQDSA-N 0.000 description 1
- PNNNRSAQSRJVSB-BXKVDMCESA-N aldehydo-L-rhamnose Chemical compound C[C@H](O)[C@H](O)[C@@H](O)[C@@H](O)C=O PNNNRSAQSRJVSB-BXKVDMCESA-N 0.000 description 1
- OXNGKCPRVRBHPO-VIXGDSECSA-N alpha-L-Fucp-(1->2)-beta-D-Galp-(1->3)-[alpha-L-Fucp-(1->4)]-D-GlcNAc Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](O[C@H]2[C@@H]([C@@H](CO)OC(O)[C@@H]2NC(C)=O)O[C@H]2[C@H]([C@H](O)[C@H](O)[C@H](C)O2)O)O[C@H](CO)[C@H](O)[C@@H]1O OXNGKCPRVRBHPO-VIXGDSECSA-N 0.000 description 1
- BNABBHGYYMZMOA-AHIHXIOASA-N alpha-maltoheptaose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@H](O[C@H](O[C@@H]3[C@H](O[C@H](O[C@@H]4[C@H](O[C@H](O[C@@H]5[C@H](O[C@H](O[C@@H]6[C@H](O[C@H](O)[C@H](O)[C@H]6O)CO)[C@H](O)[C@H]5O)CO)[C@H](O)[C@H]4O)CO)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O BNABBHGYYMZMOA-AHIHXIOASA-N 0.000 description 1
- LUEWUZLMQUOBSB-ZLBHSGTGSA-N alpha-maltotetraose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@H](O[C@H](O[C@@H]3[C@H](O[C@H](O)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-ZLBHSGTGSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000003622 anti-hsv Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 108010006523 asialoglycoprotein receptor Proteins 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 235000013532 brandy Nutrition 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 235000013736 caramel Nutrition 0.000 description 1
- 102000023852 carbohydrate binding proteins Human genes 0.000 description 1
- 108091008400 carbohydrate binding proteins Proteins 0.000 description 1
- 108010085318 carboxymethylcellulase Proteins 0.000 description 1
- FYGDTMLNYKFZSV-ZWSAEMDYSA-N cellotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-ZWSAEMDYSA-N 0.000 description 1
- 108091008394 cellulose binding proteins Proteins 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 108010057052 chitotriosidase Proteins 0.000 description 1
- 239000012501 chromatography medium Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 230000024203 complement activation Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 239000003398 denaturant Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 102000025772 fructose binding proteins Human genes 0.000 description 1
- 108091009112 fructose binding proteins Proteins 0.000 description 1
- 102000025817 fucose binding proteins Human genes 0.000 description 1
- 108010015750 fucose-binding lectin Proteins 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 108010090623 galactose binding protein Proteins 0.000 description 1
- 102000021529 galactose binding proteins Human genes 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 235000013531 gin Nutrition 0.000 description 1
- 229960002442 glucosamine Drugs 0.000 description 1
- 102000036202 glucose binding proteins Human genes 0.000 description 1
- 108091011004 glucose binding proteins Proteins 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 150000002411 histidines Chemical class 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 102000033736 lactose binding proteins Human genes 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 238000012125 lateral flow test Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 235000020121 low-fat milk Nutrition 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 150000002703 mannose derivatives Chemical class 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000006151 minimal media Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 125000001446 muramyl group Chemical group N[C@@H](C=O)[C@@H](O[C@@H](C(=O)*)C)[C@H](O)[C@H](O)CO 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 210000001322 periplasm Anatomy 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 235000013533 rum Nutrition 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 230000029305 taxis Effects 0.000 description 1
- 235000013529 tequila Nutrition 0.000 description 1
- 238000012956 testing procedure Methods 0.000 description 1
- 108091005703 transmembrane proteins Proteins 0.000 description 1
- 102000035160 transmembrane proteins Human genes 0.000 description 1
- FLUADVWHMHPUCG-SWPIJASHSA-N verbascose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]4[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O4)O)O3)O)O2)O)O1 FLUADVWHMHPUCG-SWPIJASHSA-N 0.000 description 1
- 235000015041 whisky Nutrition 0.000 description 1
- 229920001221 xylan Polymers 0.000 description 1
- 150000004823 xylans Chemical class 0.000 description 1
- 108091016328 xylose binding proteins Proteins 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
- G01N33/54388—Immunochromatographic test strips based on lateral flow
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Pathology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Emergency Medicine (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Nutrition Science (AREA)
- Polymers & Plastics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
- 2 Lateral flow device (LFD); lateral flow device system; gradient lateral flow device
- 10 Saccharide marker; marker
- 20 Test Sample
- 22 Ligand containing marker and contaminants
- 24 Contaminants
- 30 Indicator member
- 32 Column
- 34 Column frit; first frit; second frit
- 36 Gel
- 38 Saccharide-specific binding protein; large molecule; marker binding protein
- 40 Conjugate of saccharide-specific binding protein bound to indicator member
- 42 Saccharide-specific binding protein bound to saccharide and to indicator member
- 44 Saccharide-specific binding protein bound to saccharide marker
- 46 Saccharide-specific binding protein immobilized on gel
- 47 Saccharide-specific binding protein immobilized on gel and bound to saccharide marker
- 50 T-line molecule bound to saccharide or saccharide analogue
- 60 Antibody to saccharide-specific binding protein
- 70 LFD test sample application position
- 80 LFD position for holding
mobile conjugate 40 - 90 LFD position for device-bound T-line molecule
- 100 LFD position for device bound C-line antibody
- 110 Direction of lateral flow
- 120 Absorbent pad serving as a sink
SEQ ID NO:1 |
MKIKTGARIL ALSALTTMMF SASALAKIEE |
60 | |
VTVEHPDKLE EKFPQVAATG DGPDIIFWAH |
120 | |
DAVRYNGKLI AYPIAVEALS LIYNKDLLPN PPKTWEEIPA LDKELKAKGK SALMFNLQEP | 180 | |
YFTWPLIAAD GGYAFKYENG KYDIKDVGVD NAGAKAGLTF LVDLIKNKHM NADTDYSIAE | 240 | |
AAFNKGETAM TINGPWAWSN IDTSKVNYGV TVLPTFKGQP SKPFVGVLSA GINAASPNKE | 300 | |
LAKEFLENYL LTDEGLEAVN KDKPLGAVAL KSYEEELAKD PRIAATMENA QKGEIMPNIP | 360 | |
QMSAFWYAVR TAVINAASGR QTVDEALKDA QTNSSSVDKL AAALEHHHHH H |
TABLE 1 | ||
Saccharide Binding | ||
Saccharide Binding | Protein Supplemental | |
Protein | Saccharide Marker | Information |
Family 6 CBM | Cellobiose, xylopentaose | |
(carbohydrate binding | ||
proteins) from Cellvibrio | ||
mixtus | ||
CBPA | Cellulose | multidomain, exhibits |
carboxymethyl cellulase | ||
activity | ||
CbtA | Cellobiose, cellotriose, | Hyperthermophile protein |
cellotetraose | ||
agpA | mellibiose, raffinose | Rhizobium meliloti agpA |
MPB, MBL | d-mannose, N- | |
acetylglucoasmine, L-Fucose | ||
Annexin V | bisecting glycosaminoglycans | |
RTB | Galactose | Plant Lectins: Castor Bean |
GNA | Mannose | Plant Lectins: Snowdrop |
ConA | Mannose/glucosamine | Plant Lectins: Canavalis |
enisformis | ||
DBL | N-acetylgalactosamine | Plant Lectins: Dolichus |
biflorus | ||
BL | N-acetylglucosamine | Plant Lectins: Barley |
MAH | Sialic Acid | Plant Lectins: Maackia |
amurensis | ||
ASGP-R | N-acetylgalactosamine | hepatocyte receptor |
Triatennary | ||
Man/GalNAc-4-SO4- | mannose, fucose etc | transmembrane protein |
receptor (Cell surface | ||
receptor) | ||
PAPG | glycolipids with galabiose | bacterial pilus tip protein |
sequence (Glaα4Gal) | ||
Verotoxin | Glaα4Galβ4GlcβCer | Escherichia coli |
BabA | Lewis B antigen | H. pylori |
(Fucα2Galβ3(Fucα4)GlcNAcβ | ||
SabA | Sialic Acid | H. pylori |
Galectin | β-D-galactopyranoside; lactose, | |
N-acetyllactosamine | ||
VIP36 | high mannose type glycans | MDCK cells |
SaBP | Sialic acid | |
XylF | Xylose | |
ConA | Mannose | |
Galectin-1, LGALS1 | β-Galactosidase | |
Aga16B and Aga86E | 3,6-anhydro-α-L-galactose- | |
family 6 CBMs | (1,3)-β-Dgalactopyranose(1,4) | |
(Disaccharide Agarose) | ||
ELAM-1 | terminally sialylated | endothelial Leukocyte |
lactosylceramides | adhesion molecule-1. | |
with a variable number of N- | ||
acetyllactosamine repeats and | ||
at least one fucosylated N- | ||
acetylglucosamine residue | ||
Serum mannan-binding | mannose and N- | component of the |
protein | acetylglucosamine | complement activation |
pathway | ||
FimH Protein | Mannose and it's oligomers | bacterial pilus protein |
CL-46 | N-acetyl-D-glucosamine and N- | Bovine C-type lectin |
acetyl-D-mannosamine | domain connected to | |
collagen | ||
Immulectin-2 | xylose, glucose, | Insect C-type lectin |
lipopolysaccharide and mannan | (requires Ca2+) | |
Chitotriosidase | chitin and chitooligosaccharides | Mammalian lectin/chitinase |
(inactivated) | (YM1 and HCgp39/family | |
18 chitinases | ||
Gliolectin | N-acetylglucosamine-terminated | Dorsophila derived lectin |
Drosophila glycans | involved in the mediation | |
of neuronal cell pathway | ||
formation | ||
CBP30 | galactose | S-type lectin isolated from |
baby hamster kidney cells | ||
rCBP35 | Galβ1, 4GlcNAcβ1, 3Galβ1, 4- | S-type lectin from |
GlcNAc | mammalian tissue | |
RBP (receptor-binding | Glucopyranosyl saccharides, | from Lactococcus lactis |
protein) | galactose, and muramyl | The KD's ~0.26-0.13 μM |
dipeptide | tested in solution | |
(MurNAc-D-Ala-D-iGln) | ||
CfCBM2b-1-2 and related | Xylan and multimers | From C. fimi. |
proteins | ||
VP8 | Sialic acid containing human | From a rotavirus strain that |
cell surface carbohydrates | infects humans. | |
family 26 CBM | Starch and α- | Bacillus halodurans; the |
(BhCBM26), | glucooligosaccharide | CBM's are from a modular |
and a family 25 | (5 mods) protein | |
CBM(BhCBM25) | maltohexaose-forming | |
amylase | ||
CBPA | Cellulose | Eubacterium cellulosolvens |
cellulose-binding protein B | 5. Modular protein. | |
CBM | ||
STL-1 STL-2 | Rhamnose | Trout L-rhamnose binding |
proteins. | ||
L-Xylulose reductase | Xylulose | |
(modified to retain binding | ||
and inactivate reductase) | ||
TABLE 2 | ||
NaHCO3 buffer, pH |
pos | 5.7 | 6.3 | 6.9 | 7.1 | 7.7 | ||
1 | 0.588 | 0.815 | 0.826 | 0.853 | 0.849 | 0.809 |
0.1 | 0.693 | 0.861 | 0.879 | 0.896 | 0.855 | 0.817 |
0.01 | 0.685 | 0.465 | 0.392 | 0.397 | 0.366 | 0.387 |
0.001 | 0.572 | 0.238 | 0.219 | 0.140 | 0.159 | 0.254 |
0.0005 | 0.567 | 0.314 | 0.271 | 0.234 | 0.200 | 0.187 |
0.0002 | 0.595 | 0.264 | 0.192 | 0.154 | 0.195 | 0.208 |
0.0001 | 0.689 | 0.297 | 0.243 | 0.199 | 0.217 | 0.242 |
Neg | 0.706 | 0.279 | 0.212 | 0.285 | 0.263 | 0.340 |
TABLE 3 | ||
Na2CO3 buffer, pH |
Pos | 8.3 | 8.9 | 9.3 | 9.9 | 10 | 11 | ||
1 | 0.588 | 0.748 | 0.787 | 0.788 | 0.868 | 0.918 | 0.908 |
0.1 | 0.693 | 0.870 | 0.847 | 0.828 | 0.849 | 0.793 | 0.845 |
0.01 | 0.685 | 0.440 | 0.502 | 0.438 | 0.452 | 0.432 | 0.512 |
0.001 | 0.572 | 0.216 | 0.248 | 0.179 | 0.241 | 0.160 | 0.193 |
0.0005 | 0.567 | 0.233 | 0.175 | 0.152 | 0.192 | 0.183 | 0.215 |
0.0002 | 0.595 | 0.181 | 0.170 | 0.169 | 0.182 | 0.224 | 0.183 |
0.0001 | 0.689 | 0.215 | 0.215 | 0.196 | 0.235 | 0.237 | 0.185 |
Neg | 0.706 | 0.200 | 0.245 | 0.206 | 0.172 | 0.210 | 0.182 |
Claims (12)
Priority Applications (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11/768,766 US7972859B2 (en) | 2006-06-27 | 2007-06-26 | Authentication of ingestible products using saccharides as markers |
PCT/US2007/072255 WO2008002987A2 (en) | 2006-06-27 | 2007-06-27 | Authentication of ingestible products using saccharides as markers |
DE602007013046T DE602007013046D1 (en) | 2006-06-27 | 2007-06-27 | AUTHENTICATION OF APPROPRIATE PRODUCTS WITH SACCHARIDES AS A MARKER |
EP07799095A EP2038647B1 (en) | 2006-06-27 | 2007-06-27 | Authentication of ingestible products using saccharides as markers |
AP2008004728A AP2008004728A0 (en) | 2006-06-27 | 2007-06-27 | Authentication of ingestible products using saccarides as markers |
EA200970062A EA200970062A1 (en) | 2006-06-27 | 2007-06-27 | DETERMINATION OF THE AUTHENTICITY OF CONSUMED PRODUCTS USING SUCHARIDES AS MARKERS |
AT07799095T ATE501435T1 (en) | 2006-06-27 | 2007-06-27 | AUTHENTICATION OF INGESTED PRODUCTS USING SACCHARIDES AS MARKERS |
CA002655921A CA2655921A1 (en) | 2006-06-27 | 2007-06-27 | Authentication of ingestible products using saccharides as markers |
CO08137596A CO6170389A2 (en) | 2006-06-27 | 2008-12-29 | AUTHENTICATION OF EDIBLE PRODUCTS USING SUCCESSES AS MARKERS |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US81681206P | 2006-06-27 | 2006-06-27 | |
US11/768,766 US7972859B2 (en) | 2006-06-27 | 2007-06-26 | Authentication of ingestible products using saccharides as markers |
Publications (2)
Publication Number | Publication Date |
---|---|
US20070298502A1 US20070298502A1 (en) | 2007-12-27 |
US7972859B2 true US7972859B2 (en) | 2011-07-05 |
Family
ID=38617348
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/768,766 Active 2028-01-06 US7972859B2 (en) | 2006-06-27 | 2007-06-26 | Authentication of ingestible products using saccharides as markers |
Country Status (9)
Country | Link |
---|---|
US (1) | US7972859B2 (en) |
EP (1) | EP2038647B1 (en) |
AP (1) | AP2008004728A0 (en) |
AT (1) | ATE501435T1 (en) |
CA (1) | CA2655921A1 (en) |
CO (1) | CO6170389A2 (en) |
DE (1) | DE602007013046D1 (en) |
EA (1) | EA200970062A1 (en) |
WO (1) | WO2008002987A2 (en) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090089175A1 (en) * | 2007-10-02 | 2009-04-02 | Nir Platek | Product evaluation system and product evaluation method |
WO2015013704A3 (en) * | 2013-07-26 | 2015-11-05 | Lost Spirits Distillery, Llc | System and method for rapid maturation of distilled spirits |
US9637712B2 (en) | 2014-01-10 | 2017-05-02 | Lost Spirits Distillery, Llc | Method for rapid maturation of distilled spirits using light and heat processes |
US9637713B2 (en) | 2014-01-10 | 2017-05-02 | Lost Spirits Distillery, Llc | Method for rapid maturation of distilled spirits using light and heat processes |
US9829491B2 (en) | 2009-10-09 | 2017-11-28 | The Research Foundation For The State University Of New York | pH-insensitive glucose indicator protein |
US10508259B2 (en) | 2014-01-10 | 2019-12-17 | Lost Spirits Technology Llc | Method for rapid maturation of distilled spirits using light, heat, and negative pressure processes |
US10705073B2 (en) | 2014-06-18 | 2020-07-07 | Ruprecht Keller | Method for identifying of a biological sample of a mammal, composition for use in this method and kit for performance of this method |
US10947488B2 (en) | 2014-01-10 | 2021-03-16 | Lost Spirits Technology Llc | Method for rapid maturation of distilled spirits using light and heat processes |
US11894119B2 (en) | 2019-07-04 | 2024-02-06 | Ruma Gmbh | Location-independent ingestion control |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100046825A1 (en) * | 2006-02-10 | 2010-02-25 | Parallel Synthesis Technologies, Inc. | Authentication and anticounterfeiting methods and devices |
JP5669340B2 (en) * | 2008-01-23 | 2015-02-12 | シスメックス株式会社 | Sample analyzer and program for sample analyzer |
RU2484827C2 (en) | 2008-11-04 | 2013-06-20 | Юниверсити Оф Кентукки Рисерч Фаундэйшн | D-tagatose compositions and methods of preventing and treating atherosclerosis, metabolic syndrome and symptoms thereof |
RU2567658C2 (en) | 2009-10-01 | 2015-11-10 | Агроскоуп Либефельд-Позьё Алп | Authentication method of dairy products |
KR20110078185A (en) * | 2009-12-30 | 2011-07-07 | 삼성전자주식회사 | Apparatus and method for authenticating product using polynucleotides |
ES2739351T3 (en) * | 2012-10-10 | 2020-01-30 | Ruprecht Keller | Markers for pharmaceutical products |
US9586204B2 (en) * | 2014-06-23 | 2017-03-07 | Xerox Corporation | Paper sensor |
SG11201702860WA (en) * | 2014-12-24 | 2017-07-28 | Exxonmobil Res & Eng Co | Methods for determining condition and quality of petroleum products |
US20160187314A1 (en) * | 2014-12-24 | 2016-06-30 | Exxonmobil Research And Engineering Company | Methods for authentication and identification of petroleum products |
US10837924B2 (en) | 2016-10-05 | 2020-11-17 | Hewlett-Packard Development Company, L.P. | Encoding information in chemical concentrations |
GB201619789D0 (en) * | 2016-11-23 | 2017-01-04 | Pepsis Ltd | Enzyme-rich malt extract formulations |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0327163A2 (en) | 1988-02-02 | 1989-08-09 | Biocode, Inc. | Detection of chemicals by immunoassay |
US5429952A (en) | 1988-02-02 | 1995-07-04 | Biocode, Inc. | Marking of products to establish identity and source |
US5710005A (en) | 1996-10-29 | 1998-01-20 | Biocode, Inc. | Analyte detection with a gradient lateral flow device |
US5776713A (en) | 1988-02-02 | 1998-07-07 | Biocode Ltd. | Marking of products to establish identity and source |
US5942444A (en) | 1997-01-27 | 1999-08-24 | Biocode, Inc. | Marking of products to establish identity, source and fate |
US6068981A (en) | 1997-10-03 | 2000-05-30 | Biocode, Inc. | Marking of orally ingested products |
WO2000055609A2 (en) | 1999-03-16 | 2000-09-21 | Cryovac, Inc. | Authentication system and methodology |
US20050239155A1 (en) | 2002-01-04 | 2005-10-27 | Javier Alarcon | Entrapped binding protein as biosensors |
US20060035288A1 (en) | 2004-07-30 | 2006-02-16 | Green Lawrence R | Methods for detection of counterfeit liquids and foods |
-
2007
- 2007-06-26 US US11/768,766 patent/US7972859B2/en active Active
- 2007-06-27 DE DE602007013046T patent/DE602007013046D1/en active Active
- 2007-06-27 EA EA200970062A patent/EA200970062A1/en unknown
- 2007-06-27 AP AP2008004728A patent/AP2008004728A0/en unknown
- 2007-06-27 WO PCT/US2007/072255 patent/WO2008002987A2/en active Application Filing
- 2007-06-27 CA CA002655921A patent/CA2655921A1/en not_active Abandoned
- 2007-06-27 EP EP07799095A patent/EP2038647B1/en not_active Not-in-force
- 2007-06-27 AT AT07799095T patent/ATE501435T1/en not_active IP Right Cessation
-
2008
- 2008-12-29 CO CO08137596A patent/CO6170389A2/en not_active Application Discontinuation
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0327163A2 (en) | 1988-02-02 | 1989-08-09 | Biocode, Inc. | Detection of chemicals by immunoassay |
US5429952A (en) | 1988-02-02 | 1995-07-04 | Biocode, Inc. | Marking of products to establish identity and source |
US5776713A (en) | 1988-02-02 | 1998-07-07 | Biocode Ltd. | Marking of products to establish identity and source |
US5710005A (en) | 1996-10-29 | 1998-01-20 | Biocode, Inc. | Analyte detection with a gradient lateral flow device |
US6140134A (en) | 1996-10-29 | 2000-10-31 | Biocode, Inc. | Analyte detection with a gradient lateral flow device |
US5942444A (en) | 1997-01-27 | 1999-08-24 | Biocode, Inc. | Marking of products to establish identity, source and fate |
US6068981A (en) | 1997-10-03 | 2000-05-30 | Biocode, Inc. | Marking of orally ingested products |
WO2000055609A2 (en) | 1999-03-16 | 2000-09-21 | Cryovac, Inc. | Authentication system and methodology |
US20050239155A1 (en) | 2002-01-04 | 2005-10-27 | Javier Alarcon | Entrapped binding protein as biosensors |
US20060035288A1 (en) | 2004-07-30 | 2006-02-16 | Green Lawrence R | Methods for detection of counterfeit liquids and foods |
Non-Patent Citations (2)
Title |
---|
Mackenzie, W.M.; "Counterfeiting and the Diageo Anti-Counterfeiting analytical Strategy" On-line-retrieved from URL: www.nabca.org/media/161-nabca.ppt; May 18, 2007; slides 3 and 4. |
Telmer, Patrick G., et al.; "Insights into the Conformational Equilibria of Maltose binding Protein by Analysis of High Affinity Mutants"; The Journal of Biological Chemistry: vol. 278, No. 36, Sep. 5, 2003. pp. 34555-34567. |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090089175A1 (en) * | 2007-10-02 | 2009-04-02 | Nir Platek | Product evaluation system and product evaluation method |
US9053491B2 (en) | 2007-10-02 | 2015-06-09 | Elady Limited | Product evaluation system and product evaluation method |
US9058615B2 (en) * | 2007-10-02 | 2015-06-16 | Elady Limited | Product evaluation system and product evaluation method |
US9829491B2 (en) | 2009-10-09 | 2017-11-28 | The Research Foundation For The State University Of New York | pH-insensitive glucose indicator protein |
WO2015013704A3 (en) * | 2013-07-26 | 2015-11-05 | Lost Spirits Distillery, Llc | System and method for rapid maturation of distilled spirits |
US9637712B2 (en) | 2014-01-10 | 2017-05-02 | Lost Spirits Distillery, Llc | Method for rapid maturation of distilled spirits using light and heat processes |
US9637713B2 (en) | 2014-01-10 | 2017-05-02 | Lost Spirits Distillery, Llc | Method for rapid maturation of distilled spirits using light and heat processes |
US10508259B2 (en) | 2014-01-10 | 2019-12-17 | Lost Spirits Technology Llc | Method for rapid maturation of distilled spirits using light, heat, and negative pressure processes |
US10947488B2 (en) | 2014-01-10 | 2021-03-16 | Lost Spirits Technology Llc | Method for rapid maturation of distilled spirits using light and heat processes |
US11339359B2 (en) | 2014-01-10 | 2022-05-24 | Lost Spirits Technology, Llc | Method for rapid maturation of distilled spirits using light and heat processes |
US10705073B2 (en) | 2014-06-18 | 2020-07-07 | Ruprecht Keller | Method for identifying of a biological sample of a mammal, composition for use in this method and kit for performance of this method |
US11894119B2 (en) | 2019-07-04 | 2024-02-06 | Ruma Gmbh | Location-independent ingestion control |
Also Published As
Publication number | Publication date |
---|---|
AP2008004728A0 (en) | 2008-12-31 |
CA2655921A1 (en) | 2008-01-03 |
WO2008002987A2 (en) | 2008-01-03 |
EP2038647A2 (en) | 2009-03-25 |
ATE501435T1 (en) | 2011-03-15 |
CO6170389A2 (en) | 2010-06-18 |
DE602007013046D1 (en) | 2011-04-21 |
EA200970062A1 (en) | 2009-06-30 |
EP2038647B1 (en) | 2011-03-09 |
US20070298502A1 (en) | 2007-12-27 |
WO2008002987A3 (en) | 2008-04-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7972859B2 (en) | Authentication of ingestible products using saccharides as markers | |
Nishiyama et al. | Bifidobacterium bifidum extracellular sialidase enhances adhesion to the mucosal surface and supports carbohydrate assimilation | |
Imberty et al. | Microbial recognition of human cell surface glycoconjugates | |
Oho et al. | Binding of salivary glycoprotein-secretory immunoglobulin A complex to the surface protein antigen of Streptococcus mutans | |
Gonçalves et al. | Characterization of white wine mannoproteins | |
Campanero-Rhodes et al. | Microarray strategies for exploring bacterial surface glycans and their interactions with glycan-binding proteins | |
Dupin et al. | Saccharomyces c erevisiae Mannoproteins That Protect Wine from Protein Haze: Evaluation of Extraction Methods and Immunolocalization | |
Waespy et al. | Carbohydrate recognition specificity of trans-sialidase lectin domain from Trypanosoma congolense | |
Noyes et al. | Quantitative high throughput analytics to support polysaccharide production process development | |
Benešová et al. | α-L-fucosidase from Paenibacillus thiaminolyticus: its hydrolytic and transglycosylation abilities | |
Cataldi et al. | Method development for the quantitative determination of lactulose in heat-treated milks by HPAEC with pulsed amperometric detection | |
Oka | Biosynthesis of galactomannans found in filamentous fungi belonging to Pezizomycotina | |
Bourne et al. | Protein-carbohydrate interactions | |
Yu et al. | Photoaffinity probes for studying carbohydrate biology | |
Han et al. | Physicochemical properties of turanose and its potential applications as a sucrose substitute | |
Strazzulli et al. | Introducing transgalactosylation activity into a family 42 β-galactosidase | |
Harms et al. | Binding specificity of influenza C-virus to variably O-acetylated glycoconjugates and its use for histochemical detection of N-acetyl-9-O-acetylneuraminic acid in mammalian tissues | |
Heine et al. | Methods of in vitro study of galectin-glycomaterial interaction | |
PL209568B1 (en) | Method for identifying and extracting endotoxin | |
US8551719B1 (en) | Endotoxin detection method | |
Srivastava et al. | Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome | |
CA2589195A1 (en) | Particle-based multiplex assay for identifying glycosylation | |
CN101526533A (en) | Colloid gold chromatographic test paper strip and preparation method thereof for fast detecting sibutramine | |
Yabe et al. | Engineering a versatile tandem repeat-type α2-6sialic acid-binding lectin | |
WO2005005955A2 (en) | Specific detection of chitin using chitin-binding domain |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: AUTHENTIX, INC., TEXAS Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:EASTWOOD, IAN;CARTWRIGHT, JARED;DORLAND, ERWIN;AND OTHERS;REEL/FRAME:019844/0975;SIGNING DATES FROM 20070822 TO 20070828 Owner name: AUTHENTIX, INC., TEXAS Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:EASTWOOD, IAN;CARTWRIGHT, JARED;DORLAND, ERWIN;AND OTHERS;SIGNING DATES FROM 20070822 TO 20070828;REEL/FRAME:019844/0975 |
|
AS | Assignment |
Owner name: SILICON VALLEY BANK, AS ADMINISTRATIVE AGENT, TEXA Free format text: SECURITY AGREEMENT;ASSIGNOR:AUTHENTIX, INC.;REEL/FRAME:020817/0742 Effective date: 20080418 |
|
STCF | Information on status: patent grant |
Free format text: PATENTED CASE |
|
CC | Certificate of correction | ||
FEPP | Fee payment procedure |
Free format text: PAT HOLDER NO LONGER CLAIMS SMALL ENTITY STATUS, ENTITY STATUS SET TO UNDISCOUNTED (ORIGINAL EVENT CODE: STOL); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY |
|
FPAY | Fee payment |
Year of fee payment: 4 |
|
MAFP | Maintenance fee payment |
Free format text: PAYMENT OF MAINTENANCE FEE, 8TH YEAR, LARGE ENTITY (ORIGINAL EVENT CODE: M1552); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY Year of fee payment: 8 |
|
AS | Assignment |
Owner name: SILICON VALLEY BANK, CALIFORNIA Free format text: SECURITY INTEREST;ASSIGNOR:AUTHENTIX, INC.;REEL/FRAME:052446/0279 Effective date: 20200420 |
|
AS | Assignment |
Owner name: AUTHENTIX, INC., TEXAS Free format text: TERMINATION AND RELEASE OF AMENDED AND RESTATED PATENT SECURITY AGREEMENT RECORDED AT REEL 052446, FRAME 0279;ASSIGNOR:SILICON VALLEY BANK;REEL/FRAME:061201/0760 Effective date: 20220815 Owner name: LLOYDS BANK PLC, UNITED KINGDOM Free format text: SECURITY INTEREST;ASSIGNOR:AUTHENTIX, INC.;REEL/FRAME:061180/0018 Effective date: 20220812 |
|
MAFP | Maintenance fee payment |
Free format text: PAYMENT OF MAINTENANCE FEE, 12TH YEAR, LARGE ENTITY (ORIGINAL EVENT CODE: M1553); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY Year of fee payment: 12 |