US5523204A - Detection of nucleic acids in cells by strand displacement amplification - Google Patents
Detection of nucleic acids in cells by strand displacement amplification Download PDFInfo
- Publication number
- US5523204A US5523204A US08/165,719 US16571993A US5523204A US 5523204 A US5523204 A US 5523204A US 16571993 A US16571993 A US 16571993A US 5523204 A US5523204 A US 5523204A
- Authority
- US
- United States
- Prior art keywords
- target sequence
- amplification
- cells
- detected
- primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
Abstract
Description
__________________________________________________________________________ SEQUENCE LISTING (1) GENERAL INFORMATION: (iii) NUMBER OF SEQUENCES: 6 (2) INFORMATION FOR SEQ ID NO:1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE: (A) ORGANISM: Human immunodeficiency virus type 1 (B) STRAIN: HXB-2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1: ATAATCCACCTATCCCAGTAGGAGAAAT28 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE: (A) ORGANISM: Human immunodeficiency virus type 1 (B) STRAIN: HXB-2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: TTTGGTCCTTGTCTTATGTCCAGAATGC28 (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 41 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE: (A) ORGANISM: Human immunodeficiency virus type 1 (B) STRAIN: HXB-2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: ATCCTGGGATT AAATAAAATAGTAAGAATGTATAGCCCTAC41 (2) INFORMATION FOR SEQ ID NO:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: AATAGTCGCTTACTTG TTGACGGATAATCCTGGG34 (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: AAGTAACCGACTATTGTTGA CGGCTATACATTCT34 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE: (A) ORGANISM: Human immunodeficiency virus type 1 (B) STRAIN: HXB-2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: ACTATTTTATTTAAACC17
Claims (44)
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/165,719 US5523204A (en) | 1993-12-10 | 1993-12-10 | Detection of nucleic acids in cells by strand displacement amplification |
AU80221/94A AU696381B2 (en) | 1993-12-10 | 1994-12-06 | Detection of nucleic acids in cells by strand displacement amplification |
CA002137760A CA2137760A1 (en) | 1993-12-10 | 1994-12-09 | Detection of nucleic acids in cells by strand displacement amplification |
DE69426807T DE69426807T2 (en) | 1993-12-10 | 1994-12-12 | Nucleic acid amplification in SDA in cells |
EP94309308A EP0657548B1 (en) | 1993-12-10 | 1994-12-12 | Amplification of nucleic acids by SDA in cells |
JP6307877A JPH0833500A (en) | 1993-12-10 | 1994-12-12 | Detection of nucleic acid in cell by chain substitution amplification |
US08/531,749 US5733752A (en) | 1993-12-10 | 1995-09-21 | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/165,719 US5523204A (en) | 1993-12-10 | 1993-12-10 | Detection of nucleic acids in cells by strand displacement amplification |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/531,749 Continuation-In-Part US5733752A (en) | 1993-12-10 | 1995-09-21 | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
Publications (1)
Publication Number | Publication Date |
---|---|
US5523204A true US5523204A (en) | 1996-06-04 |
Family
ID=22600161
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/165,719 Expired - Lifetime US5523204A (en) | 1993-12-10 | 1993-12-10 | Detection of nucleic acids in cells by strand displacement amplification |
US08/531,749 Expired - Lifetime US5733752A (en) | 1993-12-10 | 1995-09-21 | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/531,749 Expired - Lifetime US5733752A (en) | 1993-12-10 | 1995-09-21 | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
Country Status (6)
Country | Link |
---|---|
US (2) | US5523204A (en) |
EP (1) | EP0657548B1 (en) |
JP (1) | JPH0833500A (en) |
AU (1) | AU696381B2 (en) |
CA (1) | CA2137760A1 (en) |
DE (1) | DE69426807T2 (en) |
Cited By (62)
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---|---|---|---|---|
US5631147A (en) * | 1995-09-21 | 1997-05-20 | Becton, Dickinson And Company | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
US5667994A (en) * | 1996-02-28 | 1997-09-16 | Becton, Dickinson And Company | Amplification and detection of mycobacterium avium complex species |
US5733752A (en) * | 1993-12-10 | 1998-03-31 | Becton, Dickinson And Company | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
US5789153A (en) * | 1994-09-26 | 1998-08-04 | Immuno Aktiengesellschaft | Method of quantitating nucleic acid |
US5876924A (en) * | 1994-06-22 | 1999-03-02 | Mount Sinai School Of Medicine | Nucleic acid amplification method hybridization signal amplification method (HSAM) |
US5882867A (en) * | 1995-06-07 | 1999-03-16 | Dade Behring Marburg Gmbh | Detection of nucleic acids by formation of template-dependent product |
US5916779A (en) * | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
US5942391A (en) * | 1994-06-22 | 1999-08-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method: ramification-extension amplification method (RAM) |
US6063604A (en) * | 1996-03-18 | 2000-05-16 | Molecular Biology Resources, Inc. | Target nucleic acid sequence amplification |
WO2001029265A1 (en) * | 1999-10-15 | 2001-04-26 | Ventana Medical Systems, Inc. | Method of detecting single gene copies in-situ |
US6235471B1 (en) | 1997-04-04 | 2001-05-22 | Caliper Technologies Corp. | Closed-loop biochemical analyzers |
US6344329B1 (en) * | 1995-11-21 | 2002-02-05 | Yale University | Rolling circle replication reporter systems |
US6444461B1 (en) | 1997-04-04 | 2002-09-03 | Caliper Technologies Corp. | Microfluidic devices and methods for separation |
US20030017491A1 (en) * | 2000-09-14 | 2003-01-23 | Zuo-Rong Shi | Chromogenic in situ hybridization methods, kits, and compositions |
US20030044822A1 (en) * | 1998-11-02 | 2003-03-06 | The Brigham And Women's Hospital, Inc. | Colorimetric in situ hybridization detection methods |
US20030064395A1 (en) * | 1997-11-19 | 2003-04-03 | The Government Of The U.S.A. As Represented By The Secretery Of The Dept. Of Health & Human Services | Methods for detecting intermolecular interactions in vivo and in vitro |
US20030104431A1 (en) * | 2001-07-15 | 2003-06-05 | Keck Graduate Institute | Nucleic acid amplification using nicking agents |
US6593086B2 (en) | 1996-05-20 | 2003-07-15 | Mount Sinai School Of Medicine Of New York University | Nucleic acid amplification methods |
US20030138800A1 (en) * | 2001-07-15 | 2003-07-24 | Keck Graduate Institute | Exponential amplification of nucleic acids using nicking agents |
US20030152932A1 (en) * | 2002-02-08 | 2003-08-14 | Gyanendra Kumar | Detection method using dissociated rolling circle amplification |
US20030152952A1 (en) * | 2001-07-15 | 2003-08-14 | Keck Graduate Institute | Methylation analysis using nicking agents |
US20030175706A1 (en) * | 1994-06-22 | 2003-09-18 | Zhang David Y. | Nucleic acid amplification methods |
US20030207267A1 (en) * | 2000-06-28 | 2003-11-06 | Lasken Roger S. | Multiply-primed amplification of nucleic acid sequences |
USRE38442E1 (en) * | 1994-06-22 | 2004-02-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method hybridization signal amplification method (HSAM) |
WO2004048596A2 (en) | 2002-11-21 | 2004-06-10 | Epicentre Technologies | Methods for using primers that encode one strand of a double-stranded promoter |
US20040121338A1 (en) * | 2002-12-19 | 2004-06-24 | Alsmadi Osama A. | Real-time detection of rolling circle amplification products |
US20040171041A1 (en) * | 2001-05-22 | 2004-09-02 | Dahl Gary A. | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
US20040191784A1 (en) * | 2003-03-31 | 2004-09-30 | Patricio Abarzua | Universal reagents for rolling circle amplification and methods of use |
US20040209298A1 (en) * | 2003-03-07 | 2004-10-21 | Emmanuel Kamberov | Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process |
US20040248105A1 (en) * | 2003-06-06 | 2004-12-09 | Gyanendra Kumar | Method of whole genome amplification with reduced artifact production |
US20040248103A1 (en) * | 2003-06-04 | 2004-12-09 | Feaver William John | Proximity-mediated rolling circle amplification |
US20050084875A1 (en) * | 1997-10-24 | 2005-04-21 | University Of Rochester | Molecular markers for the diagnosis of Alzheimer's disease |
US20050164207A1 (en) * | 2003-12-19 | 2005-07-28 | Affymetrix, Inc. | Method of oligonucleotide synthesis |
US20060014199A1 (en) * | 1997-05-13 | 2006-01-19 | Eramus Universiteit Rotterdam | Molecular detection of chromosome aberrations |
US20070072258A1 (en) * | 1999-09-29 | 2007-03-29 | American Registry Of Pathology | Ultrasound-mediated high-speed biological reaction and tissue processing |
US20070141586A1 (en) * | 2002-08-21 | 2007-06-21 | Epoch Biosciences, Inc. | Abasic site endonuclease assay |
US20080038725A1 (en) * | 2005-06-20 | 2008-02-14 | Yuling Luo | Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations |
US20080044816A1 (en) * | 2003-09-12 | 2008-02-21 | Becton, Dickinson And Company | Assay for Sars Coronavirus by Amplification and Detection of the Replicase Sequence |
US20080076123A1 (en) * | 2006-09-27 | 2008-03-27 | Helicos Biosciences Corporation | Polymerase variants for DNA sequencing |
WO2010021692A1 (en) | 2008-08-22 | 2010-02-25 | Sangamo Biosciences, Inc. | Methods and compositions for targeted single-stranded cleavage and targeted integration |
US20100120098A1 (en) * | 2008-10-24 | 2010-05-13 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
US20100136513A1 (en) * | 2003-09-12 | 2010-06-03 | Jianrong Lou | Assay for sars coronavirus by amplification and detection of nucleocapsid rna sequence |
US20100136557A1 (en) * | 2007-06-29 | 2010-06-03 | Sydney Brenner | Methods and Compositions for Isolating Nucleic Acid Sequence Variants |
US8206913B1 (en) | 2003-03-07 | 2012-06-26 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process |
EP2508529A1 (en) | 2008-10-24 | 2012-10-10 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
US8309303B2 (en) | 2005-04-01 | 2012-11-13 | Qiagen Gmbh | Reverse transcription and amplification of RNA with simultaneous degradation of DNA |
US8658361B2 (en) | 2010-10-21 | 2014-02-25 | Advanced Cell Diagnostics, Inc. | Ultra sensitive method for in situ detection of nucleic acids |
WO2015200609A1 (en) | 2014-06-26 | 2015-12-30 | Illumina, Inc. | Library preparation of tagged nucleic acid using single tube add-on protocol |
US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
US9683255B2 (en) | 2005-09-09 | 2017-06-20 | Qiagen Gmbh | Method for activating a nucleic acid for a polymerase reaction |
WO2017197027A1 (en) | 2016-05-11 | 2017-11-16 | Illumina, Inc. | Polynucleotide enrichment and amplification using argonaute systems |
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US5843640A (en) * | 1992-06-19 | 1998-12-01 | Northwestern University | Method of simultaneously detecting amplified nucleic acid sequences and cellular antigens in cells |
CA2204641A1 (en) * | 1995-09-21 | 1997-03-27 | Kenton L. Lohman | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
AU8890498A (en) * | 1997-08-26 | 1999-03-16 | Microscreen B.V. | Rapid detection and identification of micro-organisms |
IT1311896B1 (en) * | 1999-03-12 | 2002-03-20 | Istituto Oncologico Romagnolo | METHOD FOR DETERMINING SEQUENCE ALTERATIONS OF MOLECOLEDI DNA BY MULTIPLE-DYE CFLP (MD-CFLP). |
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Citations (4)
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US4888278A (en) * | 1985-10-22 | 1989-12-19 | University Of Massachusetts Medical Center | In-situ hybridization to detect nucleic acid sequences in morphologically intact cells |
US5168038A (en) * | 1988-06-17 | 1992-12-01 | The Board Of Trustees Of The Leland Stanford Junior University | In situ transcription in cells and tissues |
EP0524808A2 (en) * | 1991-07-23 | 1993-01-27 | F. Hoffmann-La Roche Ag | In situ PCR preparations, their use, and corresponding thermal cycler equipment |
US5270184A (en) * | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5523204A (en) * | 1993-12-10 | 1996-06-04 | Becton Dickinson And Company | Detection of nucleic acids in cells by strand displacement amplification |
US5631147A (en) * | 1995-09-21 | 1997-05-20 | Becton, Dickinson And Company | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
-
1993
- 1993-12-10 US US08/165,719 patent/US5523204A/en not_active Expired - Lifetime
-
1994
- 1994-12-06 AU AU80221/94A patent/AU696381B2/en not_active Ceased
- 1994-12-09 CA CA002137760A patent/CA2137760A1/en not_active Abandoned
- 1994-12-12 EP EP94309308A patent/EP0657548B1/en not_active Expired - Lifetime
- 1994-12-12 DE DE69426807T patent/DE69426807T2/en not_active Expired - Lifetime
- 1994-12-12 JP JP6307877A patent/JPH0833500A/en active Pending
-
1995
- 1995-09-21 US US08/531,749 patent/US5733752A/en not_active Expired - Lifetime
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AU696381B2 (en) | 1998-09-10 |
DE69426807D1 (en) | 2001-04-12 |
EP0657548B1 (en) | 2001-03-07 |
JPH0833500A (en) | 1996-02-06 |
CA2137760A1 (en) | 1995-06-11 |
AU8022194A (en) | 1995-06-15 |
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US5733752A (en) | 1998-03-31 |
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