|Publication number||US3883398 A|
|Publication date||13 May 1975|
|Filing date||7 May 1973|
|Priority date||7 May 1973|
|Publication number||US 3883398 A, US 3883398A, US-A-3883398, US3883398 A, US3883398A|
|Inventors||Kazuyuki Ray Ono|
|Original Assignee||Bellco Glass Inc|
|Export Citation||BiBTeX, EndNote, RefMan|
|Patent Citations (2), Referenced by (81), Classifications (7)|
|External Links: USPTO, USPTO Assignment, Espacenet|
United States Patent [1 1 Ono [ May 13, 1975 MICROCULTURE SLIDE CHAMBER  Inventor: Kazuyuki Ray Ono, Bridgeton, NJ.  Assignee: Bellco Glass, lnc., Vineland, NJ.  Filed: May 7, 1973  Appl. No.: 357,991
 US. Cl 195/127; 195/139  Int. Cl Cl2b 1/00  Field of Search l95/l39, I27
 References Cited UNITED STATES PATENTS 3,745.09] 7/1973 McCormick 195/139 3,791,933 2/1974 Moyer ct al. [95/127 Primary Examiner-Alvin E. Tanenholtz Attorney, Agent, or FirmSeidel, Gonda & Goldhammer  ABSTRACT A microculture slide chamber for simultaneously growing a plurality of mono-layer cell cultures on a slide includes a matrix which defines a plurality of generally parallel holes extending therethrough so that a plurality of wells for containing the culture bearing fluid are provided when the matrix is in abutment with a slide. Surrounding each hole on one side of the matrix is a gasket that is integral with the matrix and serves to seal the fluid in each well. A pressure plate with mating holes overlies the opposite side of each matrix and spring clips hold the plate, matrix and slide in assembled relation. A cover for the microculture slide chamber is provided.
10 Claims, 2 Drawing Figures MICROCULTURE SLIDE CHAMBER This invention relates to a microculture slide chamber. More particularly, this invention relates to a microculture slide chamber for simultaneously growing a plurality of mono-layer cell cultures on a slide or similar structure.
There are many types of laboratory tests, particularly in the biological sciences, wherein it is desirable to form cell cultures on a slide. Among these are monolayer cell cultures for which the present invention is particularly suited, although those skilled in the art will recognize that it may have other uses. For various reasons, it is desirable that two or more cell cultures be grown on the same slide. For example, an advantage of growing multiple cell cultures on the same slide is that different viruses can be used to innoculate the same type of cells or, in the alternative, one virus can be used to innoculate different types of cell cultures. There are, of course, other advantages as hereinafter described. The present invention provides a microculture slide chamber which enables such persons to gain these advantages.
Prior attempts have been made to provide microculture slide chambers for growing a plurality of cell cultures on the same slide. Among these is a tissue culture chamber-slide sold by Miles Laboratory, Inc. under Lab-Tek Catalog No. D2270. This device consists of a glass slide with a removable non-toxic gasket and an attached optically clear plastic sample chamber. The chamber-slide has proven to be quite unsatisfactory for several reasons. Among these is that the fluid occasionally leaks from chamber to chamber. Worse still, the separation of the slide from the remaining parts of the device requires the application of pressure to break the gasket seal followed by the removal of the gasket with forcep. In use, the breaking action or the removal of the gasket results in the removal of parts of the culture cells, a wholly unacceptable result.
The present invention overcomes the disadvantages of such prior art devices by providing a readily assemblable and disassemblable microculture slide chamber for simultaneously forming two or more culture cells or the like on a slide. Such cell cultures may be, but need not be, mono-layer cell cultures.
The microculture slide chamber comprising the present invention is designed so that it will have the advantages of consistency from slide to slide, convenience in use, be economical to purchase and maintain, and can be readily stored. The advantage of forming a plurality of cell cultures on a single slide is that there can be consistency in cell staining, consistent histochemistry, and identical manipulation. Such a device is convenient because it provides efficiency in processing and examination of all cells on a single slide and also allows for storing the cells in racks that can be readily identified. Economy is achieved because the microculture slide chamber is recyclable except, of course, for the slide itself.
In accordance with the present invention, the microculture slide chamber comprises a slide on which the cultures are to be grown together with a matrix made of resilient material through which extend a plurality of holes. The matrix is positioned in abutting relation to the slide so that each hole defines a well or chamber within which the culture bearing fluid can be maintained. The side of the matrix which abuts the slide includes gaskets integral therewith and surrounding each of the holes. The gaskets provide a fluid tight seal for each well thus formed preventing leakage from well to well. Overlying the opposite side of the hole is a plate serving as a pressure plate. The plate has a plurality of holes extending therethrough which holes are arranged on the plate so as to align with the matrix holes. A pair of spring clips engage the side edges of the surface of the slide and plate to retain the same in abutting relation with the matrix. A cover is provided for closing the wells while the cell cultures are being formed.
A microculture slide chamber constructed in accordance with what is described and claimed herein has many uses and applications. Among these are the following:
Screening for effects of compounds on cellular morphology, replication, viability and differentiation.
2. Study of the effects of compounds on the cellular uptake of a variety of radio isotopic substrates. This application requires the use of a polymer substrate or slide.
3. Autoradiographic studies permit the localization of radio isotopic tracers in the cells.
4. Viral titrations can be rapidly and easily performed in the chambers.
5. Cytotoxic antibody titrations can be done as well.
6. Lymphocytotoxic assays using either optical or radio-isotopic end points are easily performed.
7. lmmunoflourescence methods are especially easy with this invention. The detection of anti-nuclear antibodies (ANA) for testing for lupus erythematosis is very easy. Viral diagnosis could be accomplished in this system by titrating for immunoflourescence of the patients serum against preinfected cell mono-layers.
8. It is theoretically possible to perform the macrophage inhibition factor assay using this invention.
For the purpose of illustrating the invention, there are shown in the drawings forms which are presently preferred; it being understood, however, that this invention is not limited to the precise arrangements and instrumentalities shown.
FIG. 1 is an exploded perspective view of a microculture slide chamber in accordance with the present invention.
FIG. 2 is a transverse sectional view of the microculture slide chamber showing the same in assembled relation.
Referring now to the drawings in detail, wherein like numerals indicate like elements, there is shown a microculture slide chamber designated generally as 10.
The microculture slide chamber 10 is provided with a slide 12 which may be made of glass, as is conventional. The slide 12 is somewhat longer than the microculture slide chamber 10 so that a frosted portion 13 upon which identifying marks can be made protrudes outwarding from the cell. Overlying the slide 12 is a matrix 14 best shown in FIG. 1. The matrix 14 is made of a resilient material such as silicone rubber. Of course, other resilient materials may be substituted provided that they are autoclavable. Extending through the matrix 14 are a plurality of holes 16 in generally parallel relation to each other. Although the matrix 14 may take any form, such as circular or even toroidal, it is shown as being generally in the shape of a rectangular parallel-piped having opposed planar surfaces 18 and 20 between which the holes 16 extend. Ten holes 16 are shown extending from surface 18 to surface 20.
However, any number of such holes, as desired, may be provided.
When matrix 14 is brought into abutting relation with the upper surface of slide 12, each of the holes 16 defines a well or chamber for containing the culture bearing fluid. To be certain that the fluid does not leak from well to well, a gasket 22 surrounds each of the holes 16. The gaskets 22 are integrally formed with the matrix 14 and provide a fluid tight seal when the slide 12 is held in abutting relation with the matrix 14 under the force of the spring clips 24 and 26. The advantage of making the gaskets 22 integral with the matrix 14 is that they are simultaneously removed when the matrix is separated from the slide, thus avoiding the danger that their individual removal with forceps would result in damage to the cell cultures.
Overlying the matrix 14 is a pressure plate 28 whose function is to cooperate with the clips 24 and 26 to apply a compression force on the matrix 14 to retain the gaskets 22 in good contact with the slide 12. Plate 28 is preferably made of stainless steel or an autoclavable polymer such as polycarbonate. The plate 28 is dimensioned to have the same width as the matrix 14 just as the matrix 14 has a width approximately equal to the width of the slide 12. Moreover, the plate 28 is provided with holes 30 extending therethrough which holes are positioned in the plate 28 so as to align with the holes 16 in the matrix 14. Thus, the holes 30 permit ready access to the holes 16 and the wells thus defined when the microculture slide chamber is in its assembled condition. If desired, plate 28 may be permanently fixed to matrix 14.
Clips 24 and 26 provide a resilient means for main taining the microculture slide chamber in its assembled relation. As shown, each of the clips 24 and 26 is made of a tempered spring metal, such as stainless steel, and it is provided with curved dependent edge engaging sections 32 and 34. The edge engaging sections 32 and 34 depend from a medially curved or bent intermediate section such that the distance between the edge sections 32 and 34 is normally less than the thickness of the assembled microculture slide chamber including the slide 12, matrix 14 and plate 28 when each clip is in an unstressed condition. Each of the clips 24 and 26 slides over the edges of the slide 12 and the plate 28 and thereby compresses them toward the matrix 14, thus retaining the microculture slide chamber in its assembled relation without interferring with access to the holes 30. A cover 36 is made of polycarbonate polymer or some other autoclavable material and preferably is light transparent so that the interior of the hole 16 can be observed. Cover 36 is dimensioned to overlie the entire surface of plate 28 and, of course, the matrix 16 thereby protecting the culture fluid when placed in the holes 16.
It should be understood that the number of holes 16 in matrix 14 can be varied as desired. Moreover, the dimensions can also be varied. in one preferred embodiment, the holes 16 are sized to contain culture fluid of 0.4 ml maximum with a recommended amount of approximately O.2 ml. The slide 12 is preferably of stan dard dimensions for microscope slides which are approximately 09 mm to 1.09 mm in thickness.
In use, the microculture slide chamber is assembled and the appropriate culture bearing fluids are placed in two or more of the holes 16. The cultures are innoculated, the cover 36 is placed on top of the microculture slide chamber, and the entire device is placed in a CO incubator. As is known, the cells attach to the base of the glass slide 12 to form a monolayer cell culture at the bottom of each of the wells defined by the combination of the slide 12 and the holes 16 in the matrix 14. The cells can be innoculated with viruses, or any of the procedures outlined above can be effected.
Once the monolayer culture has been formed, it is a straightforward procedure to disassemble the microculture slide chamber, fix and stain the cells, thereafter examine them under an appropriate microscope. Cover slips can also be fastened to the cells to make a permanent record of the culture.
The use of the invention is not limited to glass microscope slides. Other types of slides, such as plastic slides to which the cells will adhere, can be used. One such plastic is a polyester sold under the trade name Melinex" and manufactured by Imperial Chemical Industries, Inc. The advantage of using a plastic is that it can be readily divided, if desired after formation of the cells. This may be advantageous when it is desirable to measure the radioactivity of each cell monolayer.
The present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof and, accordingly, reference should be made to the appended claims, rather than to the foregoing specification as indicating the scope of the invention.
1. A microculture slide chamber for growing cultures comprising:
a slide on which said cultures are to be grown;
a matrix having a plurality of generally parallel matrix holes extending therethrough, said matrix being made of a resilient material;
said matrix holes when positioned over said slide defining a plurality of wells for containing a culture bearing fluid;
a gasket surrounding the end of each matrix hole adjacent said slide, each said gasket being integral with said matrix, and each said gasket providing a fluid-tight seal between said slide and said matrix for retaining fluid in said wells;
a plate having openings therethrough aligned to permit access to said matrix holes when said plate is in overlying relation to said matrix;
resilient clip means for retaining said microculture slide chamber in assembled relation for growing cultures therein, said assembled relation comprising said slide in abutting relation with the gasket side of said matrix and said plate in abutting and aligned relation with the opposite side of said matrix, and
a cover for said microculture slide chamber.
2. A microculture slide chamber in accordance with claim 1 wherein said clip means comprise first and sec ond resilient clips for engaging the edges of said slide and plate and Compressing them into engagement with said matrix.
3. A microculture slide chamber in accordance with claim 1 wherein said matrix is made of a silicone polymer.
4. A microculture slide chamber in accordance with claim 1 wherein said matrix, said clip means. said plate and said cover are made of autoclavable substances.
5. A microculture slide chamber in accordance with claim 4 wherein said cover is made of a polycarbonate polymer.
6. A microculture slide chamber in accordance with claim 4 wherein said clip means is made of a stainless steel.
7. A microculture slide chamber in accordance with claim 4 wherein said plate is made of stainless steel.
8. A microculture slide chamber in accordance with claim 1 wherein said plate is permanently fixed to said matrix.
9. A microculture slide chamber in accordance with claim 1 wherein said slide is made of a polymer material.
10. A microculture slide chamber for growing cultures on a slide comprising:
a matrix having a plurality of generally parallel matrix holes extending therethrough, said matrix being made of a resilient material;
said matrix holes when positioned over a slide defining a plurality of chambers for containing a culture bearing fluid;
a gasket surrounding one end of each matrix hole on one side of said matrix, each said gasket being integral with said matrix, and each said gasket being capable of providing a fluid-tight seal between a slide and said matrix for retaining fluid in said chambers when a slide is placed against the gasket side of the matrix;
a pressure plate having openings therethrough aligned to permit access to said matrix holes when said plate is in overlying relation to said matrix; and
resilient clip means for retaining said microculture slide chamber in assembled relation with a slide for growing cultures on the slide, said assembled rela tion comprising a slide in abutting relation with the gasket side of said matrix and said plate in abutting and aligned relation with the opposite side of said matrix.
|Cited Patent||Filing date||Publication date||Applicant||Title|
|US3745091 *||18 Nov 1970||10 Jul 1973||Miles Lab||Biological reaction chamber apparatus|
|US3791933 *||25 Feb 1971||12 Feb 1974||Geomet||Rapid methods for assay of enzyme substrates and metabolites|
|Citing Patent||Filing date||Publication date||Applicant||Title|
|US4039247 *||12 Dec 1975||2 Aug 1977||National Research Development Corporation||Device for use in testing of fluid samples on microscope slides|
|US4284725 *||13 Aug 1976||18 Aug 1981||Dynasciences Corporation||Virus titration and identification system|
|US4396717 *||21 Jul 1981||2 Aug 1983||Biotest-Serum-Institut Gmbh||Nutrient medium carrier system|
|US4728607 *||22 Mar 1984||1 Mar 1988||J. K. And Susie L. Wadley Research Institute And Blood Bank||Miniaturized yeast identification system|
|US4847128 *||14 Jul 1986||11 Jul 1989||Wadley Technologies, Inc.||Miniaturized yeast identification system|
|US5081033 *||14 Jul 1986||14 Jan 1992||Wadley Technologies, Inc.||Miniaturized yeast identification system|
|US5192503 *||23 May 1990||9 Mar 1993||Mcgrath Charles M||Probe clip in situ assay apparatus|
|US5328843 *||26 Feb 1991||12 Jul 1994||Hitachi, Ltd.||Method for allocating cells and cell allocation device|
|US5364790 *||16 Feb 1993||15 Nov 1994||The Perkin-Elmer Corporation||In situ PCR amplification system|
|US5484731 *||26 May 1993||16 Jan 1996||Becton, Dickinson And Company||Multiwell in-vitro fertilization plate|
|US5527510 *||5 Jun 1995||18 Jun 1996||The Perkin-Elmer Corporation||In situ PCR amplification system|
|US5552321 *||24 Aug 1993||3 Sep 1996||Bioptechs Inc.||Temperature controlled culture dish apparatus|
|US5571721 *||5 May 1994||5 Nov 1996||Erie Scientific Company||Improved biological culture slide and method of making same|
|US5619428 *||31 May 1995||8 Apr 1997||Neopath, Inc.||Method and apparatus for integrating an automated system to a laboratory|
|US5675700 *||5 Jun 1995||7 Oct 1997||The Perkin-Elmer Corporation||Assembly system for in situ per amplification|
|US5681741 *||8 Sep 1995||28 Oct 1997||The Perkin-Elmer Corporation||In situ PCR amplification system|
|US6486401||22 Feb 2000||26 Nov 2002||Tekcel, Inc.||Multi well plate cover and assembly|
|US6896848 *||19 Dec 2000||24 May 2005||Tekcel, Inc.||Microplate cover assembly|
|US7063979||13 Jun 2002||20 Jun 2006||Grace Bio Labs., Inc.||Interface between substrates having microarrays and microtiter plates|
|US7662572||25 Aug 2006||16 Feb 2010||Platypus Technologies, Llc.||Compositions and liquid crystals|
|US7731811||14 Apr 2006||8 Jun 2010||Angros Lee H||Analytic substrate coating apparatus and method|
|US7731909||20 Feb 2004||8 Jun 2010||Grace Bio-Labs, Inc.||Reaction surface array diagnostic apparatus|
|US7736594||22 Jan 2003||15 Jun 2010||Grace Bio-Labs, Inc.||Reaction surface array diagnostic apparatus|
|US7842499||7 Aug 2007||30 Nov 2010||Platypus Technologies, Llc||Substrates, devices, and methods for cellular assays|
|US8006638||14 Apr 2006||30 Aug 2011||Angros Lee H||Analytic substrate coating apparatus and method|
|US8034306||28 Sep 2005||11 Oct 2011||Grace Bio-Labs, Inc.||Reaction surface array diagnostic apparatus including a flexible microtitre plate|
|US8048245||7 Jun 2010||1 Nov 2011||Angros Lee H||Analytic substrate coating method|
|US8048472||7 Jun 2010||1 Nov 2011||Angros Lee H||Analytic substrate coating method|
|US8192994||23 Nov 2009||5 Jun 2012||Angros Lee H||Method of applying a biological specimen to an analytic plate|
|US8268614||10 Nov 2004||18 Sep 2012||Platypus Technologies, Llc||Method for assaying cell movement|
|US8287822||14 May 2010||16 Oct 2012||Grace Bio-Labs, Inc.||Reaction surface array diagnostic apparatus|
|US8450116||31 May 2012||28 May 2013||Lee H. Angros||Method of applying a biological specimen to an analytic plate|
|US8470109||26 Oct 2011||25 Jun 2013||Lee H. Angros||Analytic substrate coating method|
|US8470264||26 Oct 2011||25 Jun 2013||Lee H. Angros||Analytic substrate coating method|
|US8512974||14 Sep 2012||20 Aug 2013||Platypus Technologies, Llc||Method for assaying cell movement|
|US8820378||29 Aug 2011||2 Sep 2014||Lee H. Angros||Analytic substrate coating apparatus and method|
|US8988620||31 Aug 2009||24 Mar 2015||Platypus Technologies, Llc||Liquid crystal based analyte detection|
|US9103794||8 Feb 2012||11 Aug 2015||Platypus Technologies Llc||Substrates, devices, and methods for quantitative liquid crystal assays|
|US9176029||28 May 2013||3 Nov 2015||Lee H. Angros||Method of applying a biological specimen to an analytic plate|
|US9255863||29 Aug 2014||9 Feb 2016||Lee H. Angros||Analytic substrate coating apparatus and method|
|US9568401||8 Feb 2016||14 Feb 2017||Lee H. Angros||Analytic substrate coating apparatus and method|
|US20020173033 *||17 May 2002||21 Nov 2002||Kyle Hammerick||Device and method or three-dimensional spatial localization and functional interconnection of different types of cells|
|US20030026739 *||13 Jun 2002||6 Feb 2003||Macbeath Gavin||Interface between substrates having microarrays and microtiter plates|
|US20040179973 *||22 Mar 2004||16 Sep 2004||Angros Lee H.||Analytic plate with containment border and method|
|US20050031489 *||17 Sep 2004||10 Feb 2005||Angros Lee H.||Analytic plate with containment border|
|US20060141446 *||30 Jan 2006||29 Jun 2006||Christopher Murphy||Substrates, devices, and methods for cellular assays|
|US20060141617 *||19 Nov 2003||29 Jun 2006||The Board Of Trustees Of The University Of Illinois||Multilayered microcultures|
|US20060231023 *||14 Apr 2006||19 Oct 2006||Angros Lee H||Analytic substrate coating apparatus and method|
|US20060233943 *||14 Apr 2006||19 Oct 2006||Angros Lee H||Analytic substrate coating apparatus and method|
|US20080056951 *||25 Aug 2007||6 Mar 2008||Angros Lee H||Analytic plates with markable portions and methods of use|
|US20080056952 *||24 Aug 2007||6 Mar 2008||Angros Lee H||Analytic plates with markable portions and methods of use|
|US20080160539 *||7 Aug 2007||3 Jul 2008||Platypus Technologies, Llc||Substrates, devices, and methods for cellular assays|
|US20090291195 *||29 Jul 2009||26 Nov 2009||Angros Lee H||Analytic plates with markable portions and methods of use|
|US20100009344 *||31 Aug 2009||14 Jan 2010||Platypus Technologies, Llc||Liquid crystal based analyte detection|
|US20100072272 *||22 Oct 2009||25 Mar 2010||Angros Lee H||Microscope slide coverslip and uses thereof|
|US20100073766 *||22 Oct 2009||25 Mar 2010||Angros Lee H||Microscope slide testing and identification assembly|
|US20100110541 *||22 Oct 2009||6 May 2010||Angros Lee H||Microscope slide coverslip and uses thereof|
|US20100151511 *||22 Oct 2009||17 Jun 2010||Millipore Corporation||Biological culture assembly|
|US20100248294 *||7 Jun 2010||30 Sep 2010||Angros Lee H||Analytic substrate coating method|
|US20100248295 *||7 Jun 2010||30 Sep 2010||Angros Lee H||Analytic substrate coating method|
|US20100267590 *||14 May 2010||21 Oct 2010||Grace Bio-Labs, Inc.||Reaction Surface Array Diagnostic Apparatus|
|US20100300216 *||23 Nov 2009||2 Dec 2010||Angros Lee H||Method of applying a biological specimen to an analytic plate|
|CN104614514A *||9 Jan 2014||13 May 2015||南通大学||Detachable slide incubator ensuring experimental steps to be carried out conveniently and efficiently|
|CN104614514B *||9 Jan 2014||20 Apr 2016||南通大学||实验步骤能方便高效进行的可拆卸的载玻片孵育器|
|DE2743799A1 *||29 Sep 1977||6 Apr 1978||Byrnes Sen||Schutzhandschuhe und garn zu deren herstellung|
|DE19923584A1 *||21 May 1999||7 Dec 2000||Memorec Medical Molecular Res||Incubation container for samples on object carriers, useful for carrying out polymerase chain reactions, comprising chamber formed by plate, cover and seal, containing reservoir to minimize evaporation|
|DE19923584C2 *||21 May 1999||24 Jan 2002||Memorec Medical Molecular Res||Inkubationssystem|
|EP0014007A1 *||10 Jan 1980||6 Aug 1980||J. Hinrich Dr. Peters||Biological container|
|EP0239450A1 *||26 Feb 1987||30 Sep 1987||Robert Cassou||Method and device for in vitro fecundation and incubator for fecundation, and cellular culture used in this device and this method|
|EP0681024A2 *||27 Apr 1995||8 Nov 1995||ERIE SCIENTIFIC COMPANY (a Delaware Corporation)||Improved biological culture slide and method of making same|
|EP0681024A3 *||27 Apr 1995||17 Jun 1998||ERIE SCIENTIFIC COMPANY (a Delaware Corporation)||Improved biological culture slide and method of making same|
|WO1998015656A1 *||3 Oct 1997||16 Apr 1998||University Of New Mexico||In situ hybridization slide processes|
|WO2002092778A2 *||17 May 2002||21 Nov 2002||The Board Of Trustees Of The Leland Stanford Junior University||Device and method for three-dimensional spatial localization and functional interconnection of different types of cells|
|WO2002092778A3 *||17 May 2002||30 May 2003||Univ Leland Stanford Junior||Device and method for three-dimensional spatial localization and functional interconnection of different types of cells|
|WO2004046337A2 *||19 Nov 2003||3 Jun 2004||The Board Of Trustees Of The University Of Illinois||Multilayered microcultures|
|WO2004046337A3 *||19 Nov 2003||18 Nov 2004||Tejal Ashwin Desai||Multilayered microcultures|
|WO2008021071A3 *||7 Aug 2007||9 Oct 2008||Platypus Technologies Llc||Substrates, devices, and methods for cellular assays|
|WO2008147783A2 *||21 May 2008||4 Dec 2008||Boston Scientific Limited||Apparatus and method of performing high-throughput cell-culture studies on biomaterials|
|WO2008147783A3 *||21 May 2008||22 Jan 2009||Boston Scient Scimed Inc||Apparatus and method of performing high-throughput cell-culture studies on biomaterials|
|WO2009103416A1 *||4 Feb 2009||27 Aug 2009||Eth Zurich||Multiwell culture plate for three-dimensional cultures|
|WO2010062310A1 *||22 Oct 2009||3 Jun 2010||Millipore Corporation||Biological culture assembly|
|International Classification||C12M1/20, C12M3/00|
|Cooperative Classification||C12M23/12, C12M23/04|
|European Classification||C12M23/12, C12M23/04|