US3425418A - Artificial blood vessels and method of preparing the same - Google Patents
Artificial blood vessels and method of preparing the same Download PDFInfo
- Publication number
- US3425418A US3425418A US359973A US35997364A US3425418A US 3425418 A US3425418 A US 3425418A US 359973 A US359973 A US 359973A US 35997364 A US35997364 A US 35997364A US 3425418 A US3425418 A US 3425418A
- Authority
- US
- United States
- Prior art keywords
- collagen
- tube
- fabric
- wall
- heparin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L33/00—Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
- A61L33/0005—Use of materials characterised by their function or physical properties
- A61L33/0011—Anticoagulant, e.g. heparin, platelet aggregation inhibitor, fibrinolytic agent, other than enzymes, attached to the substrate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/04—Hollow or tubular parts of organs, e.g. bladders, tracheae, bronchi or bile ducts
- A61F2/06—Blood vessels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/04—Hollow or tubular parts of organs, e.g. bladders, tracheae, bronchi or bile ducts
- A61F2/06—Blood vessels
- A61F2/062—Apparatus for the production of blood vessels made from natural tissue or with layers of living cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/34—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/507—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials for artificial blood vessels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2310/00—Prostheses classified in A61F2/28 or A61F2/30 - A61F2/44 being constructed from or coated with a particular material
- A61F2310/00005—The prosthesis being constructed from a particular material
- A61F2310/00365—Proteins; Polypeptides; Degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/236—Glycosaminoglycans, e.g. heparin, hyaluronic acid, chondroitin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
- A61L2300/406—Antibiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/42—Anti-thrombotic agents, anticoagulants, anti-platelet agents
Definitions
- An artificial blood vessel includes a tanned collagen tube strong enough to be self-supporting, and impregnated with an anti-coagulant which is covered with open mesh fabric secured to the tanned collagen by an outer wall portion of collagen in the native chemical state thin enough not to close the pores in the fabric.
- This invention relates to vascular grafts, that is, to artificial blood vessels, and to a method of preparing the same.
- vascular graft should be non-toxic, flexible, and porous. It should retain its strength permanently in intimate contact with body fluids, and should be readily accepted and incorporated into the surrounding tissue. The graft or prosthesis, moreover, should not unfavorably affect the flow of blood therethrough.
- inert fabrics In the surgical repair of blood vessels and body ducts, inert fabrics have found considerable use.
- the known inert fabric prostheses do not become parts of the body tissues. They frequently remain surrounded by a pool of serum after the healing process is terminated. We have found that the relatively low porosity of the known prostheses accounts for the observed formation of fluid pockets, and prevents the growth of repair tissue through the fabric grafts.
- the porosity of a fabric may be characterized by the volume of Water in milliliters per minute that will pass through one square centimeter of the fabric under a pressure differential of 120 millimeters mercury and this definition of porosity is being employed in this specification and the appended claims.
- the vascular fabric prostheses that have found use in surgery heretofore have a porosity of less than 2,000 milliliters per minute.
- a vascular graft of the invention thus includes a fabric of the desired porosity. Such a fabric is not in itself impervious to blood.
- the porous, non-absorbable fabric framework is rendered bloodtight by a collagen film or tube bonded to one side of the fabric.
- the fabric is exposed to the surrounding tissue thus permitting ingrowth of fibroblasts and endothelial cells which results in the attachment of the prosthesis to the host tisues.
- the collagen has considera- 3,425,418 Patented Feb. 4, 1969 ble tensile strength, is non-antigenic, and is slowly absorbed at a rate which may be controlled by partial tanning so that there is no appreciable decrease in the strength of the vessel structure while the collagen is being replaced by new body tissue.
- clotting of blood in vascular grafts of even very small internal diameter can be prevented if the interior collagen surface of a vascular prosthesis of the invention contains an anti-coagulant such as heparin.
- the anticoagulant properties of natural blood vessels are ascribed to the presence of mucopolysaccharides, particularly chondroitin sulfate B and heparitin sulfate.
- heparin is quickly adsorbed from aqueous solutions by a partly tanned collagen wall, and cannot readily be removed from the wall under ordinary physiological conditions.
- a collagen wall containing heparin has permanent anticoagulant properties.
- heparin and other anticoagulants are reversibly bound to the collagen through polar groups present in the anticoagulants and in the collagen, and that the anticoagulant is slowly released into the bloodstream over a period of days as the collagen is absorbed by the body.
- the probability of clotting is sharply reduced during the first few days after the implantation of the graft when the patient is most susceptible to this danger, and an open lumen is maintained during the deposition and organization of the fibrin lining surrounding the graft.
- Added mucopolysaccharides also increase the hydropliilic properties of the collagen, and they improve the physicochemical stability of the collagen by the presumed link between the mucopolysaccharides and the collagen molecules.
- the hydrophilic properties of the absorbable tube are also greatly enhanced by the admixture of glycerin which ensures elasticity of the tube even after tanning.
- the artificial blood vessels of the inveniton may be sterilized by radiation or by chemical means in a known manner. They may be stored over extended periods without loss of their desirable properties. Small amounts of antibiotics added to the collagen during the preparation of the vessel and contained in the adsorbable walls thereof further ensure safe storage and have the expected beneficial results after implantation.
- An outer wall portion of the collagen layer adjacent the fabric layer is preferably kept untanned, that is, in the native chemical state and amorphous.
- the untanned outer wall portion is adhered to the fabric layer, but does not obstruct access from the outside to the pores of the fabric.
- Fabrics well suited for the vascular grafts of the invention include those made from polyester fibers such as polyethylene terephthalate which are sold under the registered trademarks Dacron and Terylene.
- Polytetrafluoroethylene fibers, such as those 'known under the trademark Teflon also may be employed, but are less resilient and are not so conveniently fabricated as the polyester fabrics.
- Teflon also may be employed, but are less resilient and are not so conveniently fabricated as the polyester fabrics.
- the polytetrafluoroethylene fabrics have superior healing properties and produce thinner, more compact capsules.
- the non-absorbable fabric that reinforces the collagen may be prepared in tubular form by braiding, weaving, or knitting.
- Braided grafts lack dimensional stability and are not widely used, whereas, both woven and knitted grafts have been used clinically.
- Woven grafts must be tightly constructed or fraying and tfiber slippage become problems at implantation. -It is for this reason that woven grafts have comparatively low porosities. Knitting permits variations in the tightness or porosity without commensurate penalties in fray or slip qualities, and is the preferred method of construction.
- FIG. 1 is a perspective view of an extruded collagen tube
- FIG. 2 is a perspective view of a non-absorbable fabric tube
- FIG. 3 is a greatly enlarged view of the nonabsorbable knitted fabric that forms the reinforcing structure illustrated by FIG. 2;
- FIG. 4 is a greatly enlarged view of a section of a vascular prosthesis constructed in accordance with the present invention.
- FIG. 5 is a fragment of FIG. 4, enlarged.
- FIG. 6 is a view along the line 6-6 of FIG. 5.
- a vascular graft may be constructed in accordance with the present invention by slipping a collagen tube inside of a fabric tube.
- the collagen tube is softened by soaking in water or a dilute aqueous acid solution and is then expanded against the external fabric tube.
- a convenient way to accomplish this is to increase the pressure of the air within the tube, and thereby force the collagen into contact with the inner wall of the fabric. In so doing the collagen is forced against the fabric and into the interstices between the non-absorbable fibers.
- the structure is then air-dried whereby the inner collagen tube is stabilized in the form illustrated in FIGS. 4, 5, and 6.
- the non-absorbable fabrics may be knitted, crocheted, woven, or braided in the shape of the desired prosthesis, as a film, tube, Y-tube, etc. While the drawings illustrate a tubular structure suitable for use as a vascular graft, it will be understood that other shapes in which collagen is bonded to one side of a non-absorbable fabric characterized by a porosity not less than 10,000 milliliters per minute and not substantially more than 22,000 milliliters per minute offer similar advantages.
- the collagen present in the prostheses of the present invention may be treated with tanning agents such as formaldehyde, pyrogallol, chromium, etc., by methods well known in the art to obtain increased strength, and to control the rate at which the collagen will be absorbed.
- tanning agents such as formaldehyde, pyrogallol, chromium, etc.
- a combination fabric-collagen vascular graft may also be manufactured by constructing the tubular fabric framework of Dacron or Teflon to the desired diameter. The fabric framework is then coated on both sides with a collagen mass obtained by swelling collagen fibrils in an aqueous acid solution. The swollen collagen fibrils are then frozen in position and deswollen by dehydration in an organic solvent. Finally the collagen that lines the interior wall of the composite tube is treated with heparin.
- the mesh of the non-absorbable fabric be sufficiently open to permit the collagen fibrils to extend into and through the interstices of the fabric.
- These collagen fibrils that pass through the fabric cohere to the collagen fibrils on the other side of the fabric framework and form a unitary structure that resists delamination. It is preferred that only the interior wall of the graft be treated with heparin as the ingrowth of fibroblasts and endothelial cells appears retarded by the presence of heparin on the exterior surface of the graft.
- the improved prostheses of the present invention may also be constructed by weaving, knitting, Crocheting, or braiding together an inert non-absorbable thread or yarn with collagen yarn or collagen multifilament. Again the interior surface of this tubular structure is treated with heparin.
- heparin t0 the interior collagen wall an aqueous solution containing from 0.25% to 1% heparin may be used.
- the contact time of this solution with the collagen should be sufiicient to permit the heparin to penetrate about 70% of the distance through the wall of the tube. For the reasons indicated above it is preferred that there be no heparin on the exterior wall of the tube.
- Example I A rigid, self-supporting collagen tube having an internal diameter of about 10 millimeters and a wall thickness of about 1 millimeter was extruded by the procedure described in the Becker Patent No. 2,246,236, using a plastic collagen mass containing about 30% collagen by weight.
- the extruding mechanism was that described in the Becker Patent No. 2,115,607 and resulted in orientation of the collagen fibers to give excellent strength characteristics.
- the tube so obtained was placed within a woven Dacron tube having a porosity of 22,000 milliliters per minute and the assembly was immersed in water until the collagen became plastic.
- One end of the prosthesis was then closed and sufiicient air pressure was applied to the other end of the prosthesis to force the collagen against the inner wall of the fabric.
- the fabric-collagen tube was then airdried in an oven at 45 C. overnight without damage.
- the product so obtained is illustrated in FIGS. 4, 5, and 6.
- One end of the collagen-Dacron prosthesis was again closed and the tube was filled with an aqueous solution containing 1.0% of heparin.
- the heparin used standardized by the sheep plasma coagulation method, was found to contain 100 units of anticoagulant activity per milligram of dry material. After the heparin solution had penetrated about three-fourths of the distance through the wall of the collagen tube, the prosthesis was emptied of the heparin solution and air-dried overnight.
- the collagen-Dacron prosthesis was next tanned by immersing it for 30 seconds in a solution of 0.4 part of pyrogallol, 0.1 part .tetrasodium ethylenediamine tetraacetic acid, and 99.5 parts of water adjusted to pH 8.3 with ammonium hydroxide. It was then re-dried in an oven at 50 C. for 6 hours.
- the prosthesis was next immersed for 30 seconds in a solution of chromium (III) sulfate comprising 0.8 part of chromium as chromic oxide, 0.5 part of lactic acid 0.24 part of formaldehyde, and 98.46 parts of water, adjusted to pH 2.7 with sodium hydroxide.
- the prosthesis was again dried in an oven at 50 C. overnight.
- the finished prosthesis was sterilized by irradiation.
- Example II Fresh steer hides were washed with cold water at 13 C. or less in a rotating drum for 10 to 24 hours. After washing, the hides were defleshed with a scraping machine, and the hair and epidermis were cut off with a horizontal band knife. This preliminary cleaning was accomplished with standard tannery equipment.
- the remaining hair and poorly cleaned sections were cut off by hand and compositions were prepared from five hides.
- the hide composites were then cut into /2 to 4 square inch sections and reduced to pulp by three passes through a meat grinder, each pass being a finer grind.
- the first and second passes were through 18 and 8 millimeter holes, respectively.
- the final grind was through holes 1.5 millimeters in diameter. Special care was taken during the grinding process to keep the pulp below C. This was done by adding crushed ice to the hide sections as they were fed to the grinder.
- the ground pulp was next diluted with tap waterat 16 C. to give a smooth slurry containing dry solids. To this slurry was then added lactic acid, and the mixture was kneaded to form a homogeneous mass of swollen collagen fibers. The mixture so obtained contained about 25% by weight hide solids.
- the collagen mass was extruded as described in Example I to form a collagen tube having a diameter of about 8 millimeters and a wall thickness of about 0.4 millimeter.
- This extruded tube was placed within a Teflon fabric tube of the type illustrated in FIG. 2, characterized by a porosity of 10,000 milliliters, and expanded against the interior wall of the fabric as described in Example I.
- the vascular prosthesis so formed was tanned by immersing it in an aqueous solution containing 2% trimethylolmelamine (Solarpret), and dried in an oven at 45 C. overnight. It was further treated with heparin as in Example 1.
- Example III The deep flexor tendon of cattle was cleaned of fat, superficial non-collagenous protein and other extraneous. matter and was sliced on an electric meat-slicing rna'chine (rotary knife) in the frozen condition. The tendon sections were sliced perpendicularly to their longitudinal axis to a thickness of about 11 mils. An aliquot sample of the tendon slices was analyzed; the dry solids amounted to 36.97%.
- the sliced tendon was next treated with an enzyme solution to dissolve elastin.
- This enzyme solution was prepared by dissolving 0.15 part of ficin and 3.75 parts of ethylene diamine tetrasodium tetraacetate in 750 parts of water. Seventy-five parts of the sliced tendon was immersed in this solution which was stored at room temperature overnight. Then 2.25 parts of hydrogen peroxide were added to destroy any residual ficin.
- a glass tube having an inside diameter of about A- inch was fitted with a one-hole stopper of rubber through which a i -inch :glas rod was placed so that the glass rod extended coaxially within the glass tube.
- the glass rod was covered with a piece of rubber tubing, and a cylindrical tube of open-mesh woven Dacron about inch in diameter was slipped over the glass rod and rubber tube. The glass tube and glass rod were assembled in an upright position with the bottom of the Dacron fabric tube resting on the rubber stopper.
- the dispersion of swollen collagen fibrils, prepared as described above, was poured into the glass tube while maintaining the fabric tube in a coaxial position and equally spaced between the rubber tube and glass tube so that both sides of the fabric were coated with the collagen dispersion.
- This mold with the dispersion and fabric in place was frozen in the vertical position for at least 4 hours at -20 C.
- the mold was then placed in a static coagulation bath consisting of 2 liters of isopropanol, 30 cubic centimeters of concentrated ammonia (25 and 10 cubic centimeters of formaldehyde (37% solution) at room temperature, and the mold was maintained in the solution for 16 hours.
- the glass rod covered with the rubber tube and the formed collagen tube were then removed and placed in a dehydrating bath consisting of 2 liters of isoproponal. The collagen tube was left in this bath for an additional 16 hours to complete the dehydration.
- the rubber tube with the collagen tube on it was very carefully slid from the glass rod, and the rubber tube was removed from the interior of the collagen tube by pulling on both ends of the rubber tube, thereby stretching the rubber tube and reducing its diameter.
- the collagen tube was removed from the rubber tubing, it was plasticized in a bath consisting of 2 liters of isopropanol (10% water) containing 5% glycerine. This plasticizing operation is optional. After 24 hours in the plasticizing bath, the collagen tube was supported on a glass rod and air-dried.
- One end of the collagen-Dacron prosthesis so obtained was closed and the tube was filled with an aqueous solution containing 0.25% heparin.
- the heparin solution had penetrated three-fourths of the way through the collagen-Dacron tube, the tube was emptied and airdried.
- Example IV Hide sections of beef cattle were comrn'inuted and homogenized together with a dilute solution of sodium hydroxide for 24 hours, 60 milliliters 1/100 N NaOH being added per grams of the hide material. The homogenized mass was left standing in a refrigerator for 24 hours at a temperature between 0 and 4 C. whereupon it congealed into a soft gel.
- hyaluronic acid a mucopolysaccharide
- glycerin a mucopolysaccharide
- the gel was extruded in the manner described in Example I to form a tube of 9 millimeter external diameter and a wall thickness of 0.5 millimeter. Air was simultaneously blown into the axial cavity of the extruded tube at a pressure which was controlled between 40 and 150 mm. water column to prevent collapse of the extrudate.
- the extruded tube was dried to constant temperature at ordinary room temperature (15 to 25 C.) in a gentle stream of air.
- a very thin layer of amorphous unmodified collagen was applied to the dry outer surface by spraying from a gun, and a loosely knit tube of smooth, noncrimped Terylene was slipped over the tube in contact with the freshly deposited collagen layer.
- the fabric of conventional weft knit construction, had a permeability to water of 18,000 ml./cm. /min. at mm. Hg. It was elastically stretchable about 15% longitudinally as well as transversely. While some of its threads were adhered to the collagen tube by the freshly deposited collagen layer, the porosity of the fabric was virtually unatfected by its engagement with the resorbable material.
- the tube was filled with an aqueous 1% solution of 2,4,6-trimethoxytriazine, a commercial tanning agent commonly sold under the name Solapret.
- the tanning solution was removed after ten minutes, and the tube was washed out with water to remove residual unreacted tanning agent.
- the treatment was then repeated with aqueous 1% heparin solution which was left in contact with the inner wall of the tube for five minutes. The hepar'in penetrated about A of the collagen wall.
- the tube was emptied and air-dried overnight.
- the completed artificial blood vessel was sealed in a glass ampoule and was sterilized by exposure over five hours to gamma radiation of 2x10 roentgen.
- a sprayed or brushed outer coating of unmodified collagen may be dispensed with in such a case, but it may also be omitted where a fabric layer is applied to dried collagen, and high mechanical strength of the vascular graft is not required.
- the composition of the collagen tube may be varied as to the minor admixtures.
- Hyaluronie acid thus may be replaced by other mucopolysaccharides or mixtures thereof, such as those derived in a conventional manner from the corpus vitreum or cartilage of beef cattle.
- the mucopolysaccharides have distinctly noticeable effects on the stability of the gel when present in amounts of as little as 0.5 percent. We have not observed further desirable changes when more than 3 percent of the mucopolysaccharides were added to those naturally present in the collagen material after processing.
- Glycerin is beneficial in amounts of at least 0.5 percent, and may be increased to five percent with corresponding improvement of elasticity and plasticity of the collagenous material, even when partially tanned.
- antibiotics include chlortetracycline in amounts of 1 to 5 grams per 100 grams of the collagen mass. 0.5 to 5 grams neomycin, or 10,000 to 50,000 units bacitracin. The quantitative limits indicated are not critical nor is this list complete.
- the hardening reagent and the hardening method may be freely chosen from the tanners art.
- Synthetic tanning agents are preferred because of the very close reproducibility of their results, but we have successfully employed natural tanning extracts of Chinese gallnu'ts, oak, pine, quebracho, sumach, mimosa, and valonia.
- An artificial blood vessel of tubular laminar structure comprising, in combination:
- said outer layer having a permeability to water of not less than 10,000 milliliters per square centimeter per minute at a pressure differential of millimeters mercury column;
- said inner wall containing an amount of an anti-coagulant effective to prevent coagulation of blood in contact with said inner wall
Description
Feb. 4, 1969 M. CHVAPIL ET AL 3,425,413
ARTIFICIAL BLOOD VESSELS AND METHOD OF PREPARING THE SAME Filed April 15, 1964 United States Patent 1962, 5,644/62 U.S. Cl. 128-334 5 Claims Int. Cl. A6111 17/04; A61f l /24; A61] 17/00 ABSTRACT OF THE DISCLOSURE An artificial blood vessel includes a tanned collagen tube strong enough to be self-supporting, and impregnated with an anti-coagulant which is covered with open mesh fabric secured to the tanned collagen by an outer wall portion of collagen in the native chemical state thin enough not to close the pores in the fabric.
This application is a continuation-in-part of our copending application Ser. No. 330,221, filed on Dec. 10, 1963, and now abandoned.
This invention relates to vascular grafts, that is, to artificial blood vessels, and to a method of preparing the same.
As described in more detail in a paper published by us and on our behalf in the Journal of Surgical Research (vol. 3, No. 7, pages 358 to 368, Sept. 1963), a vascular graft should be non-toxic, flexible, and porous. It should retain its strength permanently in intimate contact with body fluids, and should be readily accepted and incorporated into the surrounding tissue. The graft or prosthesis, moreover, should not unfavorably affect the flow of blood therethrough.
In the surgical repair of blood vessels and body ducts, inert fabrics have found considerable use. The known inert fabric prostheses, however, do not become parts of the body tissues. They frequently remain surrounded by a pool of serum after the healing process is terminated. We have found that the relatively low porosity of the known prostheses accounts for the observed formation of fluid pockets, and prevents the growth of repair tissue through the fabric grafts.
The porosity of a fabric may be characterized by the volume of Water in milliliters per minute that will pass through one square centimeter of the fabric under a pressure differential of 120 millimeters mercury and this definition of porosity is being employed in this specification and the appended claims. The vascular fabric prostheses that have found use in surgery heretofore have a porosity of less than 2,000 milliliters per minute.
It has now been discovered that healing will be greatly improved and the growth of repair tissue through the fabric promoted if the porosity of the fabric is much greater, that is, in the range of 10,000 to about 22,000 milliliters per minute. A vascular graft of the invention thus includes a fabric of the desired porosity. Such a fabric is not in itself impervious to blood.
In the improved prostheses of the present invention, the porous, non-absorbable fabric framework is rendered bloodtight by a collagen film or tube bonded to one side of the fabric. The fabric is exposed to the surrounding tissue thus permitting ingrowth of fibroblasts and endothelial cells which results in the attachment of the prosthesis to the host tisues. The collagen has considera- 3,425,418 Patented Feb. 4, 1969 ble tensile strength, is non-antigenic, and is slowly absorbed at a rate which may be controlled by partial tanning so that there is no appreciable decrease in the strength of the vessel structure while the collagen is being replaced by new body tissue.
It has further been found that clotting of blood in vascular grafts of even very small internal diameter (three millimeters and less) can be prevented if the interior collagen surface of a vascular prosthesis of the invention contains an anti-coagulant such as heparin. The anticoagulant properties of natural blood vessels are ascribed to the presence of mucopolysaccharides, particularly chondroitin sulfate B and heparitin sulfate. We may therefore add these or other anticoagulants to the collagen structure as a whole, or we incorporate an anticoagulant only in the inner wall portion of the collagen tube to decrease the thrombotic effects of the graft. We have found that heparin is quickly adsorbed from aqueous solutions by a partly tanned collagen wall, and cannot readily be removed from the wall under ordinary physiological conditions. A collagen wall containing heparin has permanent anticoagulant properties.
While the present invention is not limited to any particular theory of action, it is believed that heparin and other anticoagulants are reversibly bound to the collagen through polar groups present in the anticoagulants and in the collagen, and that the anticoagulant is slowly released into the bloodstream over a period of days as the collagen is absorbed by the body. Thus, the probability of clotting is sharply reduced during the first few days after the implantation of the graft when the patient is most susceptible to this danger, and an open lumen is maintained during the deposition and organization of the fibrin lining surrounding the graft.
Added mucopolysaccharides also increase the hydropliilic properties of the collagen, and they improve the physicochemical stability of the collagen by the presumed link between the mucopolysaccharides and the collagen molecules. The hydrophilic properties of the absorbable tube are also greatly enhanced by the admixture of glycerin which ensures elasticity of the tube even after tanning.
The artificial blood vessels of the inveniton may be sterilized by radiation or by chemical means in a known manner. They may be stored over extended periods without loss of their desirable properties. Small amounts of antibiotics added to the collagen during the preparation of the vessel and contained in the adsorbable walls thereof further ensure safe storage and have the expected beneficial results after implantation.
An outer wall portion of the collagen layer adjacent the fabric layer is preferably kept untanned, that is, in the native chemical state and amorphous. The untanned outer wall portion is adhered to the fabric layer, but does not obstruct access from the outside to the pores of the fabric.
Fabrics well suited for the vascular grafts of the invention include those made from polyester fibers such as polyethylene terephthalate which are sold under the registered trademarks Dacron and Terylene. Polytetrafluoroethylene fibers, such as those 'known under the trademark Teflon also may be employed, but are less resilient and are not so conveniently fabricated as the polyester fabrics. The polytetrafluoroethylene fabrics, however, have superior healing properties and produce thinner, more compact capsules.
The non-absorbable fabric that reinforces the collagen may be prepared in tubular form by braiding, weaving, or knitting. Braided grafts lack dimensional stability and are not widely used, whereas, both woven and knitted grafts have been used clinically. Woven grafts must be tightly constructed or fraying and tfiber slippage become problems at implantation. -It is for this reason that woven grafts have comparatively low porosities. Knitting permits variations in the tightness or porosity without commensurate penalties in fray or slip qualities, and is the preferred method of construction.
Other features and many of the attendant advantages of this invention will be readily appreciated as the same becomes better understood by reference to the following detailed description when considered in connection with the accompanying drawing in which:
FIG. 1 is a perspective view of an extruded collagen tube;
FIG. 2 is a perspective view of a non-absorbable fabric tube;
FIG. 3 is a greatly enlarged view of the nonabsorbable knitted fabric that forms the reinforcing structure illustrated by FIG. 2;
FIG. 4 is a greatly enlarged view of a section of a vascular prosthesis constructed in accordance with the present invention;
FIG. 5 is a fragment of FIG. 4, enlarged; and
FIG. 6 is a view along the line 6-6 of FIG. 5.
Referring now to FIGS. 1, 2, and 4, a vascular graft may be constructed in accordance with the present invention by slipping a collagen tube inside of a fabric tube. The collagen tube is softened by soaking in water or a dilute aqueous acid solution and is then expanded against the external fabric tube. A convenient way to accomplish this is to increase the pressure of the air within the tube, and thereby force the collagen into contact with the inner wall of the fabric. In so doing the collagen is forced against the fabric and into the interstices between the non-absorbable fibers. The structure is then air-dried whereby the inner collagen tube is stabilized in the form illustrated in FIGS. 4, 5, and 6.
The non-absorbable fabrics may be knitted, crocheted, woven, or braided in the shape of the desired prosthesis, as a film, tube, Y-tube, etc. While the drawings illustrate a tubular structure suitable for use as a vascular graft, it will be understood that other shapes in which collagen is bonded to one side of a non-absorbable fabric characterized by a porosity not less than 10,000 milliliters per minute and not substantially more than 22,000 milliliters per minute offer similar advantages.
It will be understood that the collagen present in the prostheses of the present invention may be treated with tanning agents such as formaldehyde, pyrogallol, chromium, etc., by methods well known in the art to obtain increased strength, and to control the rate at which the collagen will be absorbed.
A combination fabric-collagen vascular graft may also be manufactured by constructing the tubular fabric framework of Dacron or Teflon to the desired diameter. The fabric framework is then coated on both sides with a collagen mass obtained by swelling collagen fibrils in an aqueous acid solution. The swollen collagen fibrils are then frozen in position and deswollen by dehydration in an organic solvent. Finally the collagen that lines the interior wall of the composite tube is treated with heparin.
In this type of construction, it is important that the mesh of the non-absorbable fabric be sufficiently open to permit the collagen fibrils to extend into and through the interstices of the fabric. These collagen fibrils that pass through the fabric cohere to the collagen fibrils on the other side of the fabric framework and form a unitary structure that resists delamination. It is preferred that only the interior wall of the graft be treated with heparin as the ingrowth of fibroblasts and endothelial cells appears retarded by the presence of heparin on the exterior surface of the graft.
The improved prostheses of the present invention may also be constructed by weaving, knitting, Crocheting, or braiding together an inert non-absorbable thread or yarn with collagen yarn or collagen multifilament. Again the interior surface of this tubular structure is treated with heparin.
In applying heparin t0 the interior collagen wall an aqueous solution containing from 0.25% to 1% heparin may be used. The contact time of this solution with the collagen should be sufiicient to permit the heparin to penetrate about 70% of the distance through the wall of the tube. For the reasons indicated above it is preferred that there be no heparin on the exterior wall of the tube.
The present invention is more fully described and exemplified in the following examples. It is to be understood, however, that our invention is not to be limited to any specific form of materials or conditions set forth in the examples, but is limited solely by the appended claims. Throughout the examples which follow, all quantities are expressed in parts by weight.
Example I A rigid, self-supporting collagen tube having an internal diameter of about 10 millimeters and a wall thickness of about 1 millimeter was extruded by the procedure described in the Becker Patent No. 2,246,236, using a plastic collagen mass containing about 30% collagen by weight. The extruding mechanism was that described in the Becker Patent No. 2,115,607 and resulted in orientation of the collagen fibers to give excellent strength characteristics.
The tube so obtained was placed within a woven Dacron tube having a porosity of 22,000 milliliters per minute and the assembly was immersed in water until the collagen became plastic. One end of the prosthesis was then closed and sufiicient air pressure was applied to the other end of the prosthesis to force the collagen against the inner wall of the fabric. The fabric-collagen tube was then airdried in an oven at 45 C. overnight without damage. The product so obtained is illustrated in FIGS. 4, 5, and 6.
One end of the collagen-Dacron prosthesis was again closed and the tube was filled with an aqueous solution containing 1.0% of heparin. The heparin used, standardized by the sheep plasma coagulation method, was found to contain 100 units of anticoagulant activity per milligram of dry material. After the heparin solution had penetrated about three-fourths of the distance through the wall of the collagen tube, the prosthesis was emptied of the heparin solution and air-dried overnight.
The collagen-Dacron prosthesis was next tanned by immersing it for 30 seconds in a solution of 0.4 part of pyrogallol, 0.1 part .tetrasodium ethylenediamine tetraacetic acid, and 99.5 parts of water adjusted to pH 8.3 with ammonium hydroxide. It was then re-dried in an oven at 50 C. for 6 hours.
The prosthesis was next immersed for 30 seconds in a solution of chromium (III) sulfate comprising 0.8 part of chromium as chromic oxide, 0.5 part of lactic acid 0.24 part of formaldehyde, and 98.46 parts of water, adjusted to pH 2.7 with sodium hydroxide. The prosthesis was again dried in an oven at 50 C. overnight. The finished prosthesis was sterilized by irradiation.
Example II Fresh steer hides were washed with cold water at 13 C. or less in a rotating drum for 10 to 24 hours. After washing, the hides were defleshed with a scraping machine, and the hair and epidermis were cut off with a horizontal band knife. This preliminary cleaning was accomplished with standard tannery equipment.
The remaining hair and poorly cleaned sections were cut off by hand and compositions were prepared from five hides. The hide composites were then cut into /2 to 4 square inch sections and reduced to pulp by three passes through a meat grinder, each pass being a finer grind. The first and second passes were through 18 and 8 millimeter holes, respectively. The final grind was through holes 1.5 millimeters in diameter. Special care was taken during the grinding process to keep the pulp below C. This was done by adding crushed ice to the hide sections as they were fed to the grinder.
The ground pulp was next diluted with tap waterat 16 C. to give a smooth slurry containing dry solids. To this slurry was then added lactic acid, and the mixture was kneaded to form a homogeneous mass of swollen collagen fibers. The mixture so obtained contained about 25% by weight hide solids.
.The collagen mass was extruded as described in Example I to form a collagen tube having a diameter of about 8 millimeters and a wall thickness of about 0.4 millimeter.
This extruded tube was placed within a Teflon fabric tube of the type illustrated in FIG. 2, characterized by a porosity of 10,000 milliliters, and expanded against the interior wall of the fabric as described in Example I.
The vascular prosthesis so formed was tanned by immersing it in an aqueous solution containing 2% trimethylolmelamine (Solarpret), and dried in an oven at 45 C. overnight. It was further treated with heparin as in Example 1.
Example III The deep flexor tendon of cattle was cleaned of fat, superficial non-collagenous protein and other extraneous. matter and was sliced on an electric meat-slicing rna'chine (rotary knife) in the frozen condition. The tendon sections were sliced perpendicularly to their longitudinal axis to a thickness of about 11 mils. An aliquot sample of the tendon slices Was analyzed; the dry solids amounted to 36.97%.
The sliced tendon was next treated with an enzyme solution to dissolve elastin. This enzyme solution was prepared by dissolving 0.15 part of ficin and 3.75 parts of ethylene diamine tetrasodium tetraacetate in 750 parts of water. Seventy-five parts of the sliced tendon was immersed in this solution which was stored at room temperature overnight. Then 2.25 parts of hydrogen peroxide were added to destroy any residual ficin.
To this mixture of tendon slices in about 750 parts of water were added an additional 2244 parts of water and 5.87 parts of cyanoacetic acid. The swelling solution was cooled to below 25 C. This mixture was stirred in a dis.- persion kettle at about 60 rpm. The remaining steps in the process were carried out at a temperature below about 25 C. and the temperature of the collagen dispersion was not allowed to exceed this temperature.
Stirring was continued for about 3 hours, during which time the individual collagen slices were swollen. The dispersion was homogenized by repeated passes through series-connected jets having orifices of 50 mils and mils respectively. The dispersion was then forced through a leaf filter containing three screens of #316 stainless steel. These screens were separated by A;-inch spacers and decreased in mesh size so that the dispersion first passed a l44mil screen, then a 9-mil screen, and finally a 4-mil screen. The dispersion of swollen solvated collagen fibrils so obtained analyzed 1.09% solids and had a pH of 2.52.
A glass tube having an inside diameter of about A- inch was fitted with a one-hole stopper of rubber through which a i -inch :glas rod was placed so that the glass rod extended coaxially within the glass tube. Before placing the glass rod and rubber stopper in position, the glass rod was covered with a piece of rubber tubing, and a cylindrical tube of open-mesh woven Dacron about inch in diameter was slipped over the glass rod and rubber tube. The glass tube and glass rod were assembled in an upright position with the bottom of the Dacron fabric tube resting on the rubber stopper. The dispersion of swollen collagen fibrils, prepared as described above, was poured into the glass tube while maintaining the fabric tube in a coaxial position and equally spaced between the rubber tube and glass tube so that both sides of the fabric were coated with the collagen dispersion. This mold with the dispersion and fabric in place was frozen in the vertical position for at least 4 hours at -20 C.
'The mold was then placed in a static coagulation bath consisting of 2 liters of isopropanol, 30 cubic centimeters of concentrated ammonia (25 and 10 cubic centimeters of formaldehyde (37% solution) at room temperature, and the mold was maintained in the solution for 16 hours. The glass rod covered with the rubber tube and the formed collagen tube were then removed and placed in a dehydrating bath consisting of 2 liters of isoproponal. The collagen tube was left in this bath for an additional 16 hours to complete the dehydration.
After dehydration, the rubber tube with the collagen tube on it was very carefully slid from the glass rod, and the rubber tube was removed from the interior of the collagen tube by pulling on both ends of the rubber tube, thereby stretching the rubber tube and reducing its diameter. After the collagen tube was removed from the rubber tubing, it was plasticized in a bath consisting of 2 liters of isopropanol (10% water) containing 5% glycerine. This plasticizing operation is optional. After 24 hours in the plasticizing bath, the collagen tube was supported on a glass rod and air-dried.
One end of the collagen-Dacron prosthesis so obtained was closed and the tube was filled with an aqueous solution containing 0.25% heparin. When the heparin solution had penetrated three-fourths of the way through the collagen-Dacron tube, the tube was emptied and airdried.
Example IV Hide sections of beef cattle were comrn'inuted and homogenized together with a dilute solution of sodium hydroxide for 24 hours, 60 milliliters 1/100 N NaOH being added per grams of the hide material. The homogenized mass was left standing in a refrigerator for 24 hours at a temperature between 0 and 4 C. whereupon it congealed into a soft gel.
During homogenizing, there were added 0.5 percent hyaluronic acid (a mucopolysaccharide), 3 percent glycerin, and 1 gram chlorotetracyclin per 100 grams of hide material.
The gel was extruded in the manner described in Example I to form a tube of 9 millimeter external diameter and a wall thickness of 0.5 millimeter. Air was simultaneously blown into the axial cavity of the extruded tube at a pressure which was controlled between 40 and 150 mm. water column to prevent collapse of the extrudate.
The extruded tube was dried to constant temperature at ordinary room temperature (15 to 25 C.) in a gentle stream of air. A very thin layer of amorphous unmodified collagen was applied to the dry outer surface by spraying from a gun, and a loosely knit tube of smooth, noncrimped Terylene was slipped over the tube in contact with the freshly deposited collagen layer.
The fabric, of conventional weft knit construction, had a permeability to water of 18,000 ml./cm. /min. at mm. Hg. It was elastically stretchable about 15% longitudinally as well as transversely. While some of its threads were adhered to the collagen tube by the freshly deposited collagen layer, the porosity of the fabric was virtually unatfected by its engagement with the resorbable material.
The tube was filled with an aqueous 1% solution of 2,4,6-trimethoxytriazine, a commercial tanning agent commonly sold under the name Solapret. The tanning solution was removed after ten minutes, and the tube was washed out with water to remove residual unreacted tanning agent. The treatment was then repeated with aqueous 1% heparin solution which was left in contact with the inner wall of the tube for five minutes. The hepar'in penetrated about A of the collagen wall. The tube was emptied and air-dried overnight.
The completed artificial blood vessel was sealed in a glass ampoule and was sterilized by exposure over five hours to gamma radiation of 2x10 roentgen.
The method outlined above may be modified in many respects without departing from the spirit and scope of this invention. It is not necessary fully to dry the collagen tube prior to covering the same with fabric. Actually, it is possible to encase a freshly extruded wet collagen tube in a tubular fabric envelope, and to adhere the fabric to the collagenous material by expanding the tube under internal air pressure. The pressure has to be carefully matched to the mechanical strength of the collagen tube to avoid embedding the fabric material in collagen to an extent which would make the pores of the fabric unavailable or not readily available. The use of a sprayed or brushed outer coating of unmodified collagen may be dispensed with in such a case, but it may also be omitted where a fabric layer is applied to dried collagen, and high mechanical strength of the vascular graft is not required.
The composition of the collagen tube may be varied as to the minor admixtures. Hyaluronie acid thus may be replaced by other mucopolysaccharides or mixtures thereof, such as those derived in a conventional manner from the corpus vitreum or cartilage of beef cattle. The mucopolysaccharides have distinctly noticeable effects on the stability of the gel when present in amounts of as little as 0.5 percent. We have not observed further desirable changes when more than 3 percent of the mucopolysaccharides were added to those naturally present in the collagen material after processing.
Glycerin is beneficial in amounts of at least 0.5 percent, and may be increased to five percent with corresponding improvement of elasticity and plasticity of the collagenous material, even when partially tanned.
The effects and dosages of antibiotics are well known from the literature on the use of implants of a similar nature. Merely by Way of example, it may be stated that suitable antibiotics include chlortetracycline in amounts of 1 to 5 grams per 100 grams of the collagen mass. 0.5 to 5 grams neomycin, or 10,000 to 50,000 units bacitracin. The quantitative limits indicated are not critical nor is this list complete.
While it is critically important that the resorption and swelling rates of the collagen be reduced by hardening, the hardening reagent and the hardening method may be freely chosen from the tanners art. Synthetic tanning agents are preferred because of the very close reproducibility of their results, but we have successfully employed natural tanning extracts of Chinese gallnu'ts, oak, pine, quebracho, sumach, mimosa, and valonia.
Many other modifications and variations of the present invention are possible in the light of the above teachings. It is therefore to be understood that, within the scope of the appended claims, the invention may be practiced otherwise than as specifically described.
This application is a continuation-in-part of our copending application Ser. No. 330,221 filed on Dec. 10, 1963.
We claim:
1. An artificial blood vessel of tubular laminar structure comprising, in combination:
(a) an exposed outer tubular layer of a physiologically inert fabric resistant to resorption by body fluids,
(1) said outer layer having a permeability to water of not less than 10,000 milliliters per square centimeter per minute at a pressure differential of millimeters mercury column; and
(b) a tubular composite layer essentially consisting of two laminae of collagen,
(1) one of said laminae of collagen being tanned and constituting an inner and self-supporting Wall of said vessel,
(2) said inner wall containing an amount of an anti-coagulant effective to prevent coagulation of blood in contact with said inner wall, and
(3) the other of said collagen laminae being essentially in the native chemical state and being bonded to said outer layer, said outer layer and said other lamina constituting an outer wall of said vessel.
2. A vessel as set forth in claim 1, wherein said other lamina of collagen is of a thickness sufiicient to adhere said fabric to said inner wall Without closing the pores of the fabric.
3. A vessel as set forth in claim 1, wherein said anticoagulant includes a mucopolysaccharide admixed to said collagen.
4. A vessel as set forth in claim 1, wherein said inner Wall further includes glycerin admixed to said collagen.
5. A vessel as set forth in claim 1, wherein said inner Wall further includes an antibiotic admixed to said collagen.
References Cited UNITED STATES PATENTS 3,106,483 10/1963 Kline et al 1l762.2 3,108,357 10/1963 Liebig 128-334 X 3,272,204- 10/1966 Artandi et al 128334 FOREIGN PATENTS 591,509 8/1947 Great Britain.
DALTON L. TRULUCK, Primal Examiner.
US. Cl. X.R.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CS564463 | 1963-06-15 |
Publications (1)
Publication Number | Publication Date |
---|---|
US3425418A true US3425418A (en) | 1969-02-04 |
Family
ID=5401669
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US359973A Expired - Lifetime US3425418A (en) | 1963-06-15 | 1964-04-15 | Artificial blood vessels and method of preparing the same |
Country Status (6)
Country | Link |
---|---|
US (1) | US3425418A (en) |
BE (1) | BE649183A (en) |
BR (1) | BR6355428D0 (en) |
CH (1) | CH472219A (en) |
DE (1) | DE1491218C3 (en) |
GB (1) | GB1018288A (en) |
Cited By (73)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3526005A (en) * | 1967-06-29 | 1970-09-01 | Gulf General Atomic Inc | Method of preparing an intravascular defect by implanting a pyrolytic carbon coated prosthesis |
US3688317A (en) * | 1970-08-25 | 1972-09-05 | Sutures Inc | Vascular prosthetic |
US3710400A (en) * | 1971-08-30 | 1973-01-16 | C Sparks | Graft member grown in a living body |
US3710777A (en) * | 1970-12-23 | 1973-01-16 | C Sparks | Method and apparatus for growing graft tubes in place |
US3908657A (en) * | 1973-01-15 | 1975-09-30 | Univ Johns Hopkins | System for continuous withdrawal of blood |
US3974526A (en) * | 1973-07-06 | 1976-08-17 | Dardik Irving I | Vascular prostheses and process for producing the same |
FR2310122A1 (en) * | 1975-05-09 | 1976-12-03 | Mac Gregor David | CARDIOVASCULAR PROSTHESES AND IMPLANTS, PRESENTING POROUS SYSTEMS |
US4229838A (en) * | 1977-07-01 | 1980-10-28 | Sumitomo Electric Industries, Ltd. | Vascular prosthesis having a composite structure |
US4319363A (en) * | 1978-05-23 | 1982-03-16 | Vettivetpillai Ketharanathan | Vascular prostheses |
US4321711A (en) * | 1978-10-18 | 1982-03-30 | Sumitomo Electric Industries, Ltd. | Vascular prosthesis |
WO1982003764A1 (en) * | 1981-05-08 | 1982-11-11 | Massachusetts Inst Technology | Fabrication of living blood vessels and glandular tissues |
US4416028A (en) * | 1981-01-22 | 1983-11-22 | Ingvar Eriksson | Blood vessel prosthesis |
WO1984000540A1 (en) * | 1982-08-04 | 1984-02-16 | Jolla Cancer Res Found | Polypeptide |
US4442133A (en) * | 1982-02-22 | 1984-04-10 | Greco Ralph S | Antibiotic bonding of vascular prostheses and other implants |
DE3503127A1 (en) * | 1984-01-30 | 1985-08-01 | Meadox Medicals, Inc., Oakland, N.J. | COLLAGEN-COVERED SYNTHETIC VESSEL REPLACEMENT |
DE3503126A1 (en) * | 1984-01-30 | 1985-08-01 | Meadox Medicals Inc | MEDICINAL RELEASING COLLAGEN-COVERED SYNTHETIC VESSEL REPLACEMENT |
US4539716A (en) * | 1981-03-19 | 1985-09-10 | Massachusetts Institute Of Technology | Fabrication of living blood vessels and glandular tissues |
US4629458A (en) * | 1985-02-26 | 1986-12-16 | Cordis Corporation | Reinforcing structure for cardiovascular graft |
US4670286A (en) * | 1983-09-20 | 1987-06-02 | Allied Corporation | Method of forming prosthetic devices |
US4695281A (en) * | 1983-03-25 | 1987-09-22 | Koken Co., Ltd. | Medical material |
US4743251A (en) * | 1983-12-08 | 1988-05-10 | Henry Bocquee | Vein prothesis and method for producing same |
US4744792A (en) * | 1985-01-22 | 1988-05-17 | Richards Medical Company | Middle ear ventilating tube |
US4822361A (en) * | 1985-12-24 | 1989-04-18 | Sumitomo Electric Industries, Ltd. | Tubular prosthesis having a composite structure |
US4834746A (en) * | 1983-06-06 | 1989-05-30 | Kanegafuchi Kagaku Kogyo Kabushiki Kaisha | Artificial vessel and process for preparing the same |
US4842575A (en) * | 1984-01-30 | 1989-06-27 | Meadox Medicals, Inc. | Method for forming impregnated synthetic vascular grafts |
US4915893A (en) * | 1982-07-16 | 1990-04-10 | Medtronic, Inc. | Method of preparing polyester filament material |
US4927410A (en) * | 1988-11-18 | 1990-05-22 | University Of South Florida | Method for fabricating prosthesis material |
AU603059B2 (en) * | 1985-06-28 | 1990-11-08 | Delta Biotechnology Limited | Fibronectins |
US4997440A (en) * | 1985-04-25 | 1991-03-05 | American Cyanamid Company | Vascular graft with absorbable and nonabsorbable components |
US5037377A (en) * | 1984-11-28 | 1991-08-06 | Medtronic, Inc. | Means for improving biocompatibility of implants, particularly of vascular grafts |
US5108424A (en) * | 1984-01-30 | 1992-04-28 | Meadox Medicals, Inc. | Collagen-impregnated dacron graft |
US5110852A (en) * | 1982-07-16 | 1992-05-05 | Rijksuniversiteit Te Groningen | Filament material polylactide mixtures |
US5192311A (en) * | 1988-04-25 | 1993-03-09 | Angeion Corporation | Medical implant and method of making |
US5197977A (en) * | 1984-01-30 | 1993-03-30 | Meadox Medicals, Inc. | Drug delivery collagen-impregnated synthetic vascular graft |
US5256418A (en) * | 1990-04-06 | 1993-10-26 | Organogenesis, Inc. | Collagen constructs |
WO1993021860A1 (en) * | 1992-05-07 | 1993-11-11 | Intervascular, Inc. | Non-thrombogenic vascular prosthesis |
US5413597A (en) * | 1990-12-29 | 1995-05-09 | Krajicek; Milan | Three-layer vascular prostheses |
US5480423A (en) * | 1993-05-20 | 1996-01-02 | Boston Scientific Corporation | Prosthesis delivery |
DE3546875C2 (en) * | 1984-01-30 | 1996-05-09 | Meadox Medicals Inc | Synthetic vascular grafts |
US5716660A (en) * | 1994-08-12 | 1998-02-10 | Meadox Medicals, Inc. | Tubular polytetrafluoroethylene implantable prostheses |
US5800519A (en) * | 1994-04-29 | 1998-09-01 | Kopin Corporation | Tubular medical prosthesis for use in a body lumen |
WO1998040112A1 (en) * | 1997-03-10 | 1998-09-17 | Meadox Medicals, Inc. | Self-aggregating protein compositions and use as sealants |
US5851230A (en) * | 1994-08-12 | 1998-12-22 | Meadox Medicals, Inc. | Vascular graft with a heparin-containing collagen sealant |
US5928279A (en) * | 1996-07-03 | 1999-07-27 | Baxter International Inc. | Stented, radially expandable, tubular PTFE grafts |
US5986168A (en) * | 1995-04-25 | 1999-11-16 | Nicem, Ltd. | Prosthesis containing bioabsorbable materials insolubilized without chemical reagents and method of making the same |
US6013099A (en) * | 1998-04-29 | 2000-01-11 | Medtronic, Inc. | Medical device for delivering a water-insoluble therapeutic salt or substance |
US6106454A (en) * | 1997-06-17 | 2000-08-22 | Medtronic, Inc. | Medical device for delivering localized radiation |
US6203536B1 (en) | 1997-06-17 | 2001-03-20 | Medtronic, Inc. | Medical device for delivering a therapeutic substance and method therefor |
US6391052B2 (en) | 1994-04-29 | 2002-05-21 | Scimed Life Systems, Inc. | Stent with collagen |
US6436135B1 (en) | 1974-10-24 | 2002-08-20 | David Goldfarb | Prosthetic vascular graft |
US20040030377A1 (en) * | 2001-10-19 | 2004-02-12 | Alexander Dubson | Medicated polymer-coated stent assembly |
US20040054406A1 (en) * | 2000-12-19 | 2004-03-18 | Alexander Dubson | Vascular prosthesis and method for production thereof |
US20040094873A1 (en) * | 2001-03-20 | 2004-05-20 | Alexander Dubson | Portable electrospinning device |
US20050085901A1 (en) * | 1997-03-10 | 2005-04-21 | Dan Castro | Self-aggregating protein compostions and use as sealants |
US20050283224A1 (en) * | 2004-06-22 | 2005-12-22 | Scimed Life Systems, Inc. | Implantable medical devices with antimicrobial and biodegradable matrices |
US6997949B2 (en) | 1993-04-26 | 2006-02-14 | Medtronic, Inc. | Medical device for delivering a therapeutic agent and method of preparation |
US20060118236A1 (en) * | 2004-11-29 | 2006-06-08 | House Wayne D | Implantable devices with reduced needle puncture site leakage |
US20070031607A1 (en) * | 2000-12-19 | 2007-02-08 | Alexander Dubson | Method and apparatus for coating medical implants |
US7244272B2 (en) | 2000-12-19 | 2007-07-17 | Nicast Ltd. | Vascular prosthesis and method for production thereof |
US20080200975A1 (en) * | 2004-01-06 | 2008-08-21 | Nicast Ltd. | Vascular Prosthesis with Anastomotic Member |
US20100331947A1 (en) * | 2005-02-17 | 2010-12-30 | Alon Shalev | Inflatable Medical Device |
CN104027844A (en) * | 2014-04-04 | 2014-09-10 | 张志辉 | Novel method for coating artificial blood vessel with Astragalus polysaccharide |
WO2014197768A1 (en) * | 2013-06-07 | 2014-12-11 | Baxter International Inc. | Immobilization of an active agent on a substrate using compounds including trihydroxyphenyl groups |
US9642949B2 (en) | 2013-03-15 | 2017-05-09 | Baxter International Inc. | Immobilization of an active agent on a substrate |
US9814560B2 (en) | 2013-12-05 | 2017-11-14 | W. L. Gore & Associates, Inc. | Tapered implantable device and methods for making such devices |
US10357385B2 (en) | 2015-06-05 | 2019-07-23 | W. L. Gore & Associates, Inc. | Low bleed implantable prosthesis with a taper |
CN110141681A (en) * | 2019-05-24 | 2019-08-20 | 深圳齐康医疗器械有限公司 | Cell suspension transplanting wound repair material and preparation method thereof |
CN111148483A (en) * | 2017-07-28 | 2020-05-12 | 斯特拉塔西斯公司 | Method and system for manufacturing an object with vascular properties |
US10687934B2 (en) | 2016-07-05 | 2020-06-23 | Carlos A. Alvarado | Serous membrane for ocular surface disorders |
CZ308556B6 (en) * | 2017-07-26 | 2020-11-25 | Vseobecna Fakultni Nemocnice V Praze | Composite vascular replacement and manufacturing method |
US11549012B2 (en) | 2017-07-28 | 2023-01-10 | Stratasys Ltd. | Formulations usable in additive manufacturing of a three-dimensional object made of a soft material |
US11559936B2 (en) | 2017-07-28 | 2023-01-24 | Stratasys Ltd. | Additive manufacturing processes employing a material featuring properties of a soft bodily tissue |
US11696832B2 (en) | 2017-07-28 | 2023-07-11 | Stratasys Ltd. | Method and system for fabricating object featuring properties of a hard tissue |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4280954A (en) | 1975-07-15 | 1981-07-28 | Massachusetts Institute Of Technology | Crosslinked collagen-mucopolysaccharide composite materials |
GB1515963A (en) * | 1975-07-15 | 1978-06-28 | Massachusetts Inst Technology | Crosslinked collagen-mucopolysaccharide composite materials |
DE2853614A1 (en) * | 1978-01-25 | 1979-07-26 | Bentley Lab | IMPLANT |
SE424401B (en) * | 1979-06-06 | 1982-07-19 | Bowald S | BLODKERLSPROTES |
EP0069156B1 (en) * | 1981-07-02 | 1985-05-29 | Intermedicat GmbH | Method for the preparation of transplants with higher biological stability |
IT1154510B (en) * | 1981-08-14 | 1987-01-21 | Bentley Lab | CONNECTOR DEVICE IMPLANTABLE IN THE BODY AND DEVICE OF VASCULAR IMPLANTATION ASSOCIATED WITH IT |
GB2156677A (en) * | 1984-03-31 | 1985-10-16 | Wessex Medical Group Ltd | Bioprosthetic conduits |
DE169259T1 (en) * | 1984-07-25 | 1986-04-30 | Surgical Patent Products Inc. Ltd., Panama | VESSEL PROSTHESES FOR DRY STORAGE, METHOD FOR TREATMENT AND THEIR USE IN SURGERY. |
GB8430265D0 (en) * | 1984-11-30 | 1985-01-09 | Vascutek Ltd | Vascular graft |
DE3608158A1 (en) * | 1986-03-12 | 1987-09-17 | Braun Melsungen Ag | VESSELED PROSTHESIS IMPREGNATED WITH CROSSLINED GELATINE AND METHOD FOR THE PRODUCTION THEREOF |
EP0323144A3 (en) * | 1987-12-28 | 1990-05-16 | Vyzkumny Ustav Potravinarskeho Prumyslu | Method of manufacturing at least single-layer tubular blood vessel endoprosthesis, especially of a small internal diameter, and extruding nozzle for carrying out this method |
US6129757A (en) | 1998-05-18 | 2000-10-10 | Scimed Life Systems | Implantable members for receiving therapeutically useful compositions |
DE102009037134A1 (en) | 2009-07-31 | 2011-02-03 | Aesculap Ag | Tubular implant for replacement of natural blood vessels |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB591509A (en) * | 1945-03-26 | 1947-08-20 | Raymond Nigel Roy | A soluble lumen suture support |
US3106483A (en) * | 1961-07-27 | 1963-10-08 | Us Catheter & Instr Corp | Synthetic blood vessel grafts |
US3108357A (en) * | 1962-06-20 | 1963-10-29 | William J Liebig | Compound absorbable prosthetic implants, fabrics and yarns therefor |
US3272204A (en) * | 1965-09-22 | 1966-09-13 | Ethicon Inc | Absorbable collagen prosthetic implant with non-absorbable reinforcing strands |
-
1963
- 1963-12-12 DE DE1491218A patent/DE1491218C3/en not_active Expired
- 1963-12-12 CH CH1526263A patent/CH472219A/en not_active IP Right Cessation
- 1963-12-13 BR BR155428/63A patent/BR6355428D0/en unknown
- 1963-12-16 GB GB49509/63A patent/GB1018288A/en not_active Expired
-
1964
- 1964-04-15 US US359973A patent/US3425418A/en not_active Expired - Lifetime
- 1964-06-12 BE BE649183A patent/BE649183A/xx unknown
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB591509A (en) * | 1945-03-26 | 1947-08-20 | Raymond Nigel Roy | A soluble lumen suture support |
US3106483A (en) * | 1961-07-27 | 1963-10-08 | Us Catheter & Instr Corp | Synthetic blood vessel grafts |
US3108357A (en) * | 1962-06-20 | 1963-10-29 | William J Liebig | Compound absorbable prosthetic implants, fabrics and yarns therefor |
US3272204A (en) * | 1965-09-22 | 1966-09-13 | Ethicon Inc | Absorbable collagen prosthetic implant with non-absorbable reinforcing strands |
Cited By (118)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3526005A (en) * | 1967-06-29 | 1970-09-01 | Gulf General Atomic Inc | Method of preparing an intravascular defect by implanting a pyrolytic carbon coated prosthesis |
US3688317A (en) * | 1970-08-25 | 1972-09-05 | Sutures Inc | Vascular prosthetic |
US3710777A (en) * | 1970-12-23 | 1973-01-16 | C Sparks | Method and apparatus for growing graft tubes in place |
US3710400A (en) * | 1971-08-30 | 1973-01-16 | C Sparks | Graft member grown in a living body |
US4008717A (en) * | 1973-01-15 | 1977-02-22 | The Johns Hopkins University | System for continuous withdrawal and analysis of blood |
US3908657A (en) * | 1973-01-15 | 1975-09-30 | Univ Johns Hopkins | System for continuous withdrawal of blood |
US3974526A (en) * | 1973-07-06 | 1976-08-17 | Dardik Irving I | Vascular prostheses and process for producing the same |
US6436135B1 (en) | 1974-10-24 | 2002-08-20 | David Goldfarb | Prosthetic vascular graft |
FR2310122A1 (en) * | 1975-05-09 | 1976-12-03 | Mac Gregor David | CARDIOVASCULAR PROSTHESES AND IMPLANTS, PRESENTING POROUS SYSTEMS |
US4229838A (en) * | 1977-07-01 | 1980-10-28 | Sumitomo Electric Industries, Ltd. | Vascular prosthesis having a composite structure |
US4319363A (en) * | 1978-05-23 | 1982-03-16 | Vettivetpillai Ketharanathan | Vascular prostheses |
US4321711A (en) * | 1978-10-18 | 1982-03-30 | Sumitomo Electric Industries, Ltd. | Vascular prosthesis |
US4416028A (en) * | 1981-01-22 | 1983-11-22 | Ingvar Eriksson | Blood vessel prosthesis |
US4539716A (en) * | 1981-03-19 | 1985-09-10 | Massachusetts Institute Of Technology | Fabrication of living blood vessels and glandular tissues |
WO1982003764A1 (en) * | 1981-05-08 | 1982-11-11 | Massachusetts Inst Technology | Fabrication of living blood vessels and glandular tissues |
US4546500A (en) * | 1981-05-08 | 1985-10-15 | Massachusetts Institute Of Technology | Fabrication of living blood vessels and glandular tissues |
US4442133A (en) * | 1982-02-22 | 1984-04-10 | Greco Ralph S | Antibiotic bonding of vascular prostheses and other implants |
US5110852A (en) * | 1982-07-16 | 1992-05-05 | Rijksuniversiteit Te Groningen | Filament material polylactide mixtures |
US4915893A (en) * | 1982-07-16 | 1990-04-10 | Medtronic, Inc. | Method of preparing polyester filament material |
WO1984000540A1 (en) * | 1982-08-04 | 1984-02-16 | Jolla Cancer Res Found | Polypeptide |
US4695281A (en) * | 1983-03-25 | 1987-09-22 | Koken Co., Ltd. | Medical material |
US4834746A (en) * | 1983-06-06 | 1989-05-30 | Kanegafuchi Kagaku Kogyo Kabushiki Kaisha | Artificial vessel and process for preparing the same |
US4670286A (en) * | 1983-09-20 | 1987-06-02 | Allied Corporation | Method of forming prosthetic devices |
US4743251A (en) * | 1983-12-08 | 1988-05-10 | Henry Bocquee | Vein prothesis and method for producing same |
US5197977A (en) * | 1984-01-30 | 1993-03-30 | Meadox Medicals, Inc. | Drug delivery collagen-impregnated synthetic vascular graft |
US5108424A (en) * | 1984-01-30 | 1992-04-28 | Meadox Medicals, Inc. | Collagen-impregnated dacron graft |
JPS6192672A (en) * | 1984-01-30 | 1986-05-10 | メドツクス・メデイカルズ・インコ−ポレ−テツド | Drug compounded collagen coated synthetic blood vessel implant tissue |
US4842575A (en) * | 1984-01-30 | 1989-06-27 | Meadox Medicals, Inc. | Method for forming impregnated synthetic vascular grafts |
JPS60203264A (en) * | 1984-01-30 | 1985-10-14 | メドツクス・メデイカルズ・インコ−ポレ−テツド | Synthetic vessel transplantation tissue coated with collagen |
DE3503126A1 (en) * | 1984-01-30 | 1985-08-01 | Meadox Medicals Inc | MEDICINAL RELEASING COLLAGEN-COVERED SYNTHETIC VESSEL REPLACEMENT |
JPH0636818B2 (en) | 1984-01-30 | 1994-05-18 | メドツクス・メデイカルズ・インコ−ポレ−テツド | Collagen synthetic vascular graft tissue |
DE3546875C2 (en) * | 1984-01-30 | 1996-05-09 | Meadox Medicals Inc | Synthetic vascular grafts |
JPH0636817B2 (en) | 1984-01-30 | 1994-05-18 | メドツクス・メデイカルズ・インコ−ポレ−テツド | Distributed collagen synthetic vascular graft tissue |
DE3503127A1 (en) * | 1984-01-30 | 1985-08-01 | Meadox Medicals, Inc., Oakland, N.J. | COLLAGEN-COVERED SYNTHETIC VESSEL REPLACEMENT |
US5037377A (en) * | 1984-11-28 | 1991-08-06 | Medtronic, Inc. | Means for improving biocompatibility of implants, particularly of vascular grafts |
US4744792A (en) * | 1985-01-22 | 1988-05-17 | Richards Medical Company | Middle ear ventilating tube |
US4629458A (en) * | 1985-02-26 | 1986-12-16 | Cordis Corporation | Reinforcing structure for cardiovascular graft |
US4997440A (en) * | 1985-04-25 | 1991-03-05 | American Cyanamid Company | Vascular graft with absorbable and nonabsorbable components |
AU603059B2 (en) * | 1985-06-28 | 1990-11-08 | Delta Biotechnology Limited | Fibronectins |
US4822361A (en) * | 1985-12-24 | 1989-04-18 | Sumitomo Electric Industries, Ltd. | Tubular prosthesis having a composite structure |
US5192311A (en) * | 1988-04-25 | 1993-03-09 | Angeion Corporation | Medical implant and method of making |
US4927410A (en) * | 1988-11-18 | 1990-05-22 | University Of South Florida | Method for fabricating prosthesis material |
WO1990005501A1 (en) * | 1988-11-18 | 1990-05-31 | University Of South Florida | Method for fabricating prosthesis material |
US5256418A (en) * | 1990-04-06 | 1993-10-26 | Organogenesis, Inc. | Collagen constructs |
US5413597A (en) * | 1990-12-29 | 1995-05-09 | Krajicek; Milan | Three-layer vascular prostheses |
WO1993021860A1 (en) * | 1992-05-07 | 1993-11-11 | Intervascular, Inc. | Non-thrombogenic vascular prosthesis |
US20080275544A1 (en) * | 1993-04-26 | 2008-11-06 | Medtronic, Inc. | Medical devices for delivering a therapeutic agent and method of preparation |
US7811317B2 (en) | 1993-04-26 | 2010-10-12 | Medtronic, Inc. | Medical devices for delivering a therapeutic agent and method of preparation |
US7419696B2 (en) | 1993-04-26 | 2008-09-02 | Medtronic, Inc. | Medical devices for delivering a therapeutic agent and method of preparation |
US20060085064A1 (en) * | 1993-04-26 | 2006-04-20 | Medtronic, Inc. | Medical devices for delivering a therapeutic agent and method of preparation |
US6997949B2 (en) | 1993-04-26 | 2006-02-14 | Medtronic, Inc. | Medical device for delivering a therapeutic agent and method of preparation |
US6251132B1 (en) | 1993-05-20 | 2001-06-26 | Boston Scientific Corporation | Prosthesis delivery |
US5480423A (en) * | 1993-05-20 | 1996-01-02 | Boston Scientific Corporation | Prosthesis delivery |
US5824058A (en) * | 1993-05-20 | 1998-10-20 | Boston Scientific Corporation | Prosthesis delivery |
US6656212B2 (en) | 1993-05-20 | 2003-12-02 | Scimed Life Systems, Inc. | Prosthesis delivery |
US6419694B1 (en) | 1994-04-29 | 2002-07-16 | Scimed Life Systems, Inc. | Medical prosthesis |
US5800519A (en) * | 1994-04-29 | 1998-09-01 | Kopin Corporation | Tubular medical prosthesis for use in a body lumen |
US6391052B2 (en) | 1994-04-29 | 2002-05-21 | Scimed Life Systems, Inc. | Stent with collagen |
US5716660A (en) * | 1994-08-12 | 1998-02-10 | Meadox Medicals, Inc. | Tubular polytetrafluoroethylene implantable prostheses |
US6162247A (en) * | 1994-08-12 | 2000-12-19 | Meadox Medicals, Inc. | Vascular graft impregnated with a heparin-containing collagen sealant |
US5851230A (en) * | 1994-08-12 | 1998-12-22 | Meadox Medicals, Inc. | Vascular graft with a heparin-containing collagen sealant |
US5986168A (en) * | 1995-04-25 | 1999-11-16 | Nicem, Ltd. | Prosthesis containing bioabsorbable materials insolubilized without chemical reagents and method of making the same |
US5928279A (en) * | 1996-07-03 | 1999-07-27 | Baxter International Inc. | Stented, radially expandable, tubular PTFE grafts |
US6786920B2 (en) | 1996-07-03 | 2004-09-07 | Edwards Lifesciences Corporation | Radially expandable stented tubular PTFE grafts |
US6177609B1 (en) | 1997-03-10 | 2001-01-23 | Meadox Medicals, Inc. | Self-aggregating protein compositions and use as sealants |
US20050085901A1 (en) * | 1997-03-10 | 2005-04-21 | Dan Castro | Self-aggregating protein compostions and use as sealants |
US6299639B1 (en) | 1997-03-10 | 2001-10-09 | Meadox Medicals, Inc. | Self-aggregating protein compositions and use as sealants |
US7585319B2 (en) | 1997-03-10 | 2009-09-08 | Maquet Cardiovascular, Llc | Self-aggregating protein compositions and use as sealants |
WO1998040112A1 (en) * | 1997-03-10 | 1998-09-17 | Meadox Medicals, Inc. | Self-aggregating protein compositions and use as sealants |
US6106454A (en) * | 1997-06-17 | 2000-08-22 | Medtronic, Inc. | Medical device for delivering localized radiation |
US20040111150A1 (en) * | 1997-06-17 | 2004-06-10 | Medtronic Vascular, Inc. | Medical device for delivering a therapeutic substance and method therefor |
US6730120B2 (en) | 1997-06-17 | 2004-05-04 | Medtronic, Inc. | Medical device for delivering a therapeutic substance and method therefor |
US6203536B1 (en) | 1997-06-17 | 2001-03-20 | Medtronic, Inc. | Medical device for delivering a therapeutic substance and method therefor |
US6399144B2 (en) | 1998-04-29 | 2002-06-04 | Medtronic Inc. | Medical device for delivering a therapeutic substance and method therefor |
US6013099A (en) * | 1998-04-29 | 2000-01-11 | Medtronic, Inc. | Medical device for delivering a water-insoluble therapeutic salt or substance |
US7241309B2 (en) | 1999-04-15 | 2007-07-10 | Scimed Life Systems, Inc. | Self-aggregating protein compositions and use as sealants |
US7115220B2 (en) | 2000-12-19 | 2006-10-03 | Nicast Ltd. | Vascular prosthesis and method for production thereof |
US7276271B2 (en) | 2000-12-19 | 2007-10-02 | Nicast Ltd. | Polymer fiber tubular structure having kinking resistance |
US20040054406A1 (en) * | 2000-12-19 | 2004-03-18 | Alexander Dubson | Vascular prosthesis and method for production thereof |
US7112293B2 (en) | 2000-12-19 | 2006-09-26 | Nicast Ltd. | Method and apparatus for manufacturing polymer fiber shells via electrospinning |
US20040096532A1 (en) * | 2000-12-19 | 2004-05-20 | Alexander Dubson | Polymer fiber tubular structure having kinking resistance |
US20070031607A1 (en) * | 2000-12-19 | 2007-02-08 | Alexander Dubson | Method and apparatus for coating medical implants |
US20040096533A1 (en) * | 2000-12-19 | 2004-05-20 | Alexander Dubson | Method and apparatus of improving mechanical characteristics of nonwoven materials |
US7244272B2 (en) | 2000-12-19 | 2007-07-17 | Nicast Ltd. | Vascular prosthesis and method for production thereof |
US7244116B2 (en) | 2000-12-19 | 2007-07-17 | Nicast Ltd. | Apparatus for improving mechanical characteristics of nonwoven materials |
US20040094873A1 (en) * | 2001-03-20 | 2004-05-20 | Alexander Dubson | Portable electrospinning device |
US7794219B2 (en) | 2001-03-20 | 2010-09-14 | Nicast Ltd. | Portable electrospinning device |
US20040030377A1 (en) * | 2001-10-19 | 2004-02-12 | Alexander Dubson | Medicated polymer-coated stent assembly |
US20080200975A1 (en) * | 2004-01-06 | 2008-08-21 | Nicast Ltd. | Vascular Prosthesis with Anastomotic Member |
US8192481B2 (en) | 2004-06-22 | 2012-06-05 | Boston Scientific Scimed, Inc. | Implantable medical devices with anti-microbial and biodegradable matrices |
WO2006007214A1 (en) * | 2004-06-22 | 2006-01-19 | Boston Scientific Scimed, Inc. | Composite vascular graft with antimicrobial and biodegradable matrices and an outer fabric layer |
US20050283224A1 (en) * | 2004-06-22 | 2005-12-22 | Scimed Life Systems, Inc. | Implantable medical devices with antimicrobial and biodegradable matrices |
US7794490B2 (en) | 2004-06-22 | 2010-09-14 | Boston Scientific Scimed, Inc. | Implantable medical devices with antimicrobial and biodegradable matrices |
US20100324667A1 (en) * | 2004-06-22 | 2010-12-23 | Boston Scientific Scimed, Inc. | Implantable medical devices with anti-microbial and biodegradable matrices |
US20060118236A1 (en) * | 2004-11-29 | 2006-06-08 | House Wayne D | Implantable devices with reduced needle puncture site leakage |
US8029563B2 (en) | 2004-11-29 | 2011-10-04 | Gore Enterprise Holdings, Inc. | Implantable devices with reduced needle puncture site leakage |
US8906087B2 (en) | 2004-11-29 | 2014-12-09 | W. L. Gore & Associates, Inc. | Method of making implantable devices with reduced needle puncture site leakage |
US20100331947A1 (en) * | 2005-02-17 | 2010-12-30 | Alon Shalev | Inflatable Medical Device |
US9642949B2 (en) | 2013-03-15 | 2017-05-09 | Baxter International Inc. | Immobilization of an active agent on a substrate |
US9895468B2 (en) | 2013-03-15 | 2018-02-20 | Baxter International Inc. | Immobilization of an active agent on a substrate |
US10428200B2 (en) | 2013-06-07 | 2019-10-01 | Baxter International Inc. | Immobilization of an active agent on a substrate using compounds including trihydroxyphenyl groups |
US9655917B2 (en) | 2013-06-07 | 2017-05-23 | Baxter International Inc. | Immobilization of an active agent on a substrate using compounds including trihydroxyphenyl groups |
WO2014197768A1 (en) * | 2013-06-07 | 2014-12-11 | Baxter International Inc. | Immobilization of an active agent on a substrate using compounds including trihydroxyphenyl groups |
CN105377315B (en) * | 2013-06-07 | 2018-11-09 | 巴克斯特国际公司 | Activating agent is fixed on base material using the compound including trihydroxy phenyl |
US9814560B2 (en) | 2013-12-05 | 2017-11-14 | W. L. Gore & Associates, Inc. | Tapered implantable device and methods for making such devices |
US11259910B2 (en) | 2013-12-05 | 2022-03-01 | W. L. Gore & Associates, Inc. | Tapered implantable device and methods for making such devices |
CN104027844A (en) * | 2014-04-04 | 2014-09-10 | 张志辉 | Novel method for coating artificial blood vessel with Astragalus polysaccharide |
US10357385B2 (en) | 2015-06-05 | 2019-07-23 | W. L. Gore & Associates, Inc. | Low bleed implantable prosthesis with a taper |
US11622871B2 (en) | 2015-06-05 | 2023-04-11 | W. L. Gore & Associates, Inc. | Low bleed implantable prosthesis with a taper |
US10687934B2 (en) | 2016-07-05 | 2020-06-23 | Carlos A. Alvarado | Serous membrane for ocular surface disorders |
CZ308556B6 (en) * | 2017-07-26 | 2020-11-25 | Vseobecna Fakultni Nemocnice V Praze | Composite vascular replacement and manufacturing method |
CN111148483A (en) * | 2017-07-28 | 2020-05-12 | 斯特拉塔西斯公司 | Method and system for manufacturing an object with vascular properties |
US11549012B2 (en) | 2017-07-28 | 2023-01-10 | Stratasys Ltd. | Formulations usable in additive manufacturing of a three-dimensional object made of a soft material |
US11559936B2 (en) | 2017-07-28 | 2023-01-24 | Stratasys Ltd. | Additive manufacturing processes employing a material featuring properties of a soft bodily tissue |
US11696832B2 (en) | 2017-07-28 | 2023-07-11 | Stratasys Ltd. | Method and system for fabricating object featuring properties of a hard tissue |
US11801630B2 (en) | 2017-07-28 | 2023-10-31 | Stratasys Ltd. | Method and system for fabricating object featuring properties of a blood vessel |
US11939468B2 (en) | 2017-07-28 | 2024-03-26 | Stratasys Ltd. | Formulations usable in additive manufacturing of a three-dimensional object made of a soft material |
CN110141681A (en) * | 2019-05-24 | 2019-08-20 | 深圳齐康医疗器械有限公司 | Cell suspension transplanting wound repair material and preparation method thereof |
Also Published As
Publication number | Publication date |
---|---|
CH472219A (en) | 1969-05-15 |
BE649183A (en) | 1964-10-01 |
DE1491218B2 (en) | 1972-06-08 |
DE1491218C3 (en) | 1973-01-04 |
BR6355428D0 (en) | 1973-07-19 |
DE1491218A1 (en) | 1969-04-03 |
GB1018288A (en) | 1966-01-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US3425418A (en) | Artificial blood vessels and method of preparing the same | |
US3272204A (en) | Absorbable collagen prosthetic implant with non-absorbable reinforcing strands | |
US5108424A (en) | Collagen-impregnated dacron graft | |
US4822361A (en) | Tubular prosthesis having a composite structure | |
US6696074B2 (en) | Processing fetal or neo-natal tissue to produce a scaffold for tissue engineering | |
US5037377A (en) | Means for improving biocompatibility of implants, particularly of vascular grafts | |
CN1105795C (en) | Collagen material and process for producing the same | |
JP3504389B2 (en) | Tubular graft impregnated with collagen sealant containing heparin | |
JP3799626B2 (en) | Cardiovascular repair material and method for producing the same | |
US5028597A (en) | Antithrombogenic materials | |
CA1234653A (en) | Biomaterial | |
CA1264207A (en) | Collagen synthetic vascular graft composite | |
JPH0636817B2 (en) | Distributed collagen synthetic vascular graft tissue | |
CN1237889A (en) | Artificial blood vessel | |
AU2002309898A1 (en) | EB matrix production from fetal tissues and its use for tissue repair | |
JP4968976B2 (en) | Collagen material and production method thereof | |
EP0246638A2 (en) | Biologically modified synthetic grafts | |
US3918099A (en) | Artificial organ or part or section thereof for implanation into the human body and method of producing the same | |
JPH0240341B2 (en) | ||
EP0282091A2 (en) | Medical device with heparin slow-release | |
JPS5861744A (en) | Implant material for repairing living body | |
JPH0732798B2 (en) | Composite structure tubular organ prosthesis | |
CA2448436C (en) | Eb matrix production from fetal tissues and its use for tissue repair | |
JP2010029684A (en) | Collagen material and process for producing the same | |
JP2607266B2 (en) | Medical materials |