US20040045825A1 - Cartridge for electrophoresis detection device and manufacturing method thereof - Google Patents
Cartridge for electrophoresis detection device and manufacturing method thereof Download PDFInfo
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- US20040045825A1 US20040045825A1 US10/435,587 US43558703A US2004045825A1 US 20040045825 A1 US20040045825 A1 US 20040045825A1 US 43558703 A US43558703 A US 43558703A US 2004045825 A1 US2004045825 A1 US 2004045825A1
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- 238000001962 electrophoresis Methods 0.000 title claims abstract description 27
- 238000001514 detection method Methods 0.000 title claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 claims description 17
- 238000001746 injection moulding Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 description 9
- 238000005251 capillar electrophoresis Methods 0.000 description 7
- 238000000926 separation method Methods 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 4
- 239000000499 gel Substances 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
Definitions
- the invention relates to a cartridge and a manufacturing method thereof; in particular, to a cartridge for an electrophoresis detection device and a manufacturing method thereof.
- FIG. 1 shows a conventional gel electrophoresis apparatus 10 .
- the apparatus 10 comprises a separation tank 11 , and a power supply 12 electrically coupled thereto.
- a sample is applied near one edge of a layer of gelatin carried on a flexible sheet, usually called a “gel” 13 .
- the gel 13 is disposed in the separation tank 11 , undergoes electrophoresis by the power supply 12 , is stained and the density of the resulting pattern is measured to reveal the proteins contained in the sample.
- capillary electrophoresis is a more recent development and can be used to perform the type of electrophoresis separations presently performed with gels.
- FIG. 2 shows a conventional capillary electrophoresis analyzer 20 described in U.S. Pat. No. 5,560,811.
- An electrophoresis separation plate 21 has a plurality of sample wells 22 at one end and a common buffer reservoir 23 at the other end.
- a first master electrode 25 is electrically connected to a cell electrode 26 in the sample wells 22 .
- a second master electrode 27 is in the common buffer reservoir 23 .
- Capillary electrophoresis columns 28 are mounted in the plate 21 such that there is electrical communication between the first master electrode 25 by way of the capillary electrophoresis column 28 when the sample wells 22 and the reservoir 23 are filled with electrically conductive liquid. In operation, current between the master electrodes permits electrophoresis of the sample from the sample well 22 to the reservoir 23 .
- the apparatus 100 comprises a main portion 110 and a hand-held electrophoresis detection device 120 . Since the hand-held electrophoresis detection device 120 is connected to the main portion 110 in a separable manner, it can be operated conveniently without the limitation of the location of the main portion 110 . Thus, the detection can be performed more quickly and conveniently.
- the main portion 110 controls a detection of the sample.
- the hand-held electrophoresis detection device 120 comprises a support 121 , a cartridge 122 , and a plurality of detection columns 123 .
- the support 121 is used as a basic element of the device 120 , and is electrically connected to the main portion 110 .
- the cartridge 122 is disposed in the support 121 in a detachable manner, and is electrically connected to the support 122 . After detection, the cartridge 122 can be replaced quickly such that contamination between the samples is avoided.
- the detection columns 123 are disposed on the cartridge 122 , and the samples are attached thereto. Thus, the samples can be moved in the detection columns 123 to perform the detection.
- the invention provides a cartridge for an electrophoresis detection device and a manufacturing method thereof.
- the invention provides a cartridge for an electrophoresis detection device.
- the cartridge comprises a first member and a second member.
- the first member comprises a first concave portion.
- the second member comprises a second concave portion corresponding to the first concave portion.
- the second member is combined with the first member in a manner such that the second concave portion faces the first concave portion.
- the first member comprises a first protrusion and a third concave portion, formed on the first protrusion and communicating with the first concave portion.
- the second member comprises a second protrusion and a fourth concave portion, formed on the second protrusion and communicating with the second concave portion.
- the first protrusion comprises a first end at which the third concave portion communicates with the first concave portion and a second end opposite the first end, and the width of the first end exceeds that of the second end
- the second protrusion comprises a third end at which the fourth concave portion communicates with the second concave portion and a fourth end opposite the third end, the width of the third end exceeding that of the fourth end.
- the cartridge further comprises a capillary disposed in the first concave portion and the second concave portion in a manner such that the capillary protrudes from the first and second members.
- one end of the capillary, protruding from the first and second members, is tapered.
- the capillary comprises a plurality of through holes formed near an end protruding from the first and second members.
- the cartridge further comprises an electrode disposed between the first and second members in a manner such that the electrode is exposed by the first and second members.
- the first member comprises a fifth concave portion
- the second member comprises a sixth concave portion
- the electrode is disposed in the fifth concave portion and the sixth concave portion.
- the first member comprises a seventh concave portion communicating with the first concave portion and the fifth concave portion
- the second member comprises an eighth concave portion communicating with the second concave portion and the sixth concave portion.
- the invention provides a method for manufacturing a cartridge for an electrophoresis detection device.
- the method comprises the following steps. First, a first member, formed with an integral first concave portion, is formed. Then, a second member, formed with an integral second concave portion, is formed. Finally, the first and second members are combined in a manner such that the second concave portion faces the first concave portion.
- first and second members may be formed by several manufacturing methods, for example, by injection molding.
- the invention provides another method for manufacturing a cartridge for an electrophoresis detection device.
- the method comprises the following steps. First, a first member, formed with an integral first concave portion, is formed, and a second member, formed with an integral second concave portion, is formed. Then, a capillary is provided, and is disposed in the first concave portion. Finally, the first and second members are combined in a manner such that the second concave portion faces the first concave portion. Also, the capillary is disposed in the first concave portion and the second concave portion in a manner such that the capillary protrudes from the first and second members.
- FIG. 1 is a schematic view of a conventional gel electrophoresis apparatus
- FIG. 2 is a schematic view of a conventional capillary electrophoresis analyzer as described in U.S. Pat. No. 5,560,811;
- FIG. 3 is a schematic view of a conventional electrophoresis separation apparatus
- FIG. 4 a is a schematic view of a cartridge for an electrophoresis detection device as disclosed in a first embodiment of the invention
- FIG. 4 b is a combined cartridge in FIG. 4 a;
- FIG. 5 is a flowchart of a method for manufacturing the cartridge in FIG. 4 a;
- FIG. 6 a is a schematic view of a cartridge for an electrophoresis detection device as disclosed in a second embodiment of the invention.
- FIG. 6 b is a combined cartridge in FIG. 6 a;
- FIG. 7 a is a variant embodiment of a capillary in FIG. 6 a;
- FIG. 7 b is another variant embodiment of a capillary in FIG. 6 a ;
- FIG. 8 is a flowchart of a method for manufacturing the cartridge in FIG. 6 a.
- the cartridge 200 comprises a first member 210 , a second member 220 , and an electrode 230 .
- the first member 210 comprises a plurality of first concave portions 211 , a plurality of first protrusions 212 , a plurality of third concave portions 213 , a fifth concave portion 214 , and a seventh concave portion 215 .
- the first concave portions 211 are formed around the center portion of the first member 210 .
- Each of the first protrusions 212 is formed at the lower portion of the first member 210 , and corresponds to the first concave portions 211 respectively.
- Each of the third concave portions 213 is formed on the corresponding first protrusion 212 , and communicates with the corresponding first concave portion 211 respectively.
- the fifth concave portion 214 is formed at the upper portion of the first member 210 , and electrode 230 is disposed thereon.
- the seventh concave portion 215 communicates with the first concave portions 211 and the fifth concave portion 214 , with a predetermined liquid disposed therein such that the liquid is in contact with the electrode 230 .
- the second member 220 comprises a plurality of second concave portions 221 , a plurality of second protrusions 222 , a plurality of fourth concave portions 223 , a sixth concave portion 224 , and an eighth concave portion 225 .
- the second concave portions 221 are formed around the center portion of the second member 220 .
- Each of the second protrusions 222 is formed at the lower portion of the second member 220 , and corresponds to the second concave portions 221 respectively.
- Each of the fourth concave portions 223 is formed on the corresponding second protrusion 222 , and communicates with the corresponding second concave portion 221 respectively.
- the sixth concave portion 224 is formed at the upper portion of the second member 220 , and electrode 230 is disposed thereon.
- the eighth concave portion 225 communicates with the second concave portions 221 and the sixth concave portion 224 , with a predetermined liquid disposed therein such that the liquid is in contact with the electrode 230 .
- the first protrusion 212 comprises a first end 212 a at which the third concave portion 213 communicates with the first concave portion 211 and a second end 212 b opposite the first end 212 a .
- the width W 1 of the first end 212 a exceeds the width W 2 of the second end 212 b .
- the second protrusion 222 comprises a third end 222 a at which the fourth concave portion 223 communicates with the second concave portion 221 and a fourth end 222 b opposite the third end 222 a .
- the width W 3 of the third end 222 a exceeds the width W 4 of the fourth end 222 b .
- the electrode 230 is disposed between the first member 210 and the second member 220 in a manner such that the electrode 230 is exposed by the first member 210 and the second member 220 .
- the electrode 230 can be electrically connected with the support 121 .
- step S 11 a first member 210 as shown in FIG. 4 a is formed, and the first concave portions 211 are integrally formed thereon.
- step S 12 a second member 220 as shown in FIG. 4 a is formed, whereon the second concave portions 221 are integrally formed.
- step S 13 the first member 210 and the second member 220 are combined in a manner such that the second concave portions 221 face the first concave portions 211 .
- the electrode 230 is located between the first member 210 and the second member 220 as shown in FIG. 4 b.
- first member 210 and the second member 220 may be formed by injection molding.
- first member 210 and the second member 220 may be of polymer.
- the cartridge 300 comprises a first member 310 , a second member 320 , an electrode 330 , and a plurality of capillaries 340 .
- the first member 310 comprises a plurality of first concave portions 311 , a fifth concave portion 314 , and a seventh concave portion 315 .
- the first concave portions 311 are formed around the center portion of the first member 310 .
- the fifth concave portion 314 is formed at the upper portion of the first member 310 , and electrode 330 is disposed thereon.
- the seventh concave portion 315 communicates with the first concave portions 311 and the fifth concave portion 314 , with a predetermined liquid disposed therein such that the liquid is in contact with the electrode 330 .
- the second member 320 comprises a plurality of second concave portions 321 , a sixth concave portion 324 , and an eighth concave portion 325 .
- the second concave portions 321 are formed around the center portion of the second member 320 .
- the sixth concave portion 324 is formed at the upper portion of the second member 320 , and electrode 330 is disposed thereon.
- the eighth concave portion 325 communicates with the second concave portions 321 and the sixth concave portion 324 , with a predetermined liquid disposed therein such that the liquid is in contact with the electrode 330 .
- the electrode 330 is disposed between the first member 310 and the second member 320 in a manner such that the electrode 330 is exposed by the first member 310 and the second member 320 .
- the electrode 330 is electrically connected with the support 121 .
- the capillaries 340 are used as the channels of the cartridge 300 ; that is, the attached samples flow therethrough. Each of the capillaries 340 is disposed in the first concave portion 311 and the second concave portion 321 in a manner such that it protrudes from the first member 310 and the second member 320 .
- the structure of the capillary 340 can be changed to increase the volume of the attached sample.
- one end, protruding from the first member 310 and the second member 320 , of the capillary 340 a forms a tapered portion 341 .
- the area of the tip end of the capillary 340 a is increased such that the volume of the attached sample can be increased.
- the cartridge 340 b is formed with a plurality of through holes 342 near the end protruding from the first member 310 and the second member 320 .
- step S 21 a first member 310 as shown in FIG. 6 a is formed, with the first concave portions 311 integrally formed thereon.
- step S 22 a second member 320 as shown in FIG. 6 a is formed, with the second concave portions 321 integrally formed thereon.
- step 23 a plurality of capillaries 340 as shown in FIG. 6 a are provided in the first concave portions 321 .
- step S 24 the first member 310 and the second member 320 are combined in a manner such that the second concave portions 321 face the first concave portions 311 .
- the electrode 330 and the capillaries 340 are located between the first member 310 and the second member 320 as shown in FIG. 6 b.
Abstract
A cartridge for an electrophoresis detection device and a manufacturing method thereof. The cartridge includes a first member, a second member, and a capillary. The first member includes a first concave portion. The second member includes a second concave portion corresponding to the first concave portion. The second member is combined with the first member in a manner such that the second concave portion faces the first concave portion. The capillary is disposed in the first concave portion and the second concave portion in a manner such that it protrudes from the first and second members.
Description
- 1. Field of the Invention
- The invention relates to a cartridge and a manufacturing method thereof; in particular, to a cartridge for an electrophoresis detection device and a manufacturing method thereof.
- 2. Description of the Related Art
- The value of electrophoresis in biochemistry has been recognized for some time in the analysis, for example, of proteins in serum as well as other body fluids. FIG. 1 shows a conventional
gel electrophoresis apparatus 10. Theapparatus 10 comprises aseparation tank 11, and apower supply 12 electrically coupled thereto. During analysis, a sample is applied near one edge of a layer of gelatin carried on a flexible sheet, usually called a “gel” 13. Thegel 13 is disposed in theseparation tank 11, undergoes electrophoresis by thepower supply 12, is stained and the density of the resulting pattern is measured to reveal the proteins contained in the sample. - Although gel electrophoresis is relatively inexpensive in terms of the supplies and equipment required to perform sample analyses, the technique requires skilled technicians and is time consuming, effectively resulting in a high cost per test and limiting the number of tests that can be performed using the technique.
- Efforts have been made to automate electrophoresis in the clinical laboratories. For example, capillary electrophoresis is a more recent development and can be used to perform the type of electrophoresis separations presently performed with gels.
- Automated forms of capillary electrophoresis analyzers are known in the art. For example, FIG. 2 shows a conventional capillary electrophoresis analyzer20 described in U.S. Pat. No. 5,560,811. An
electrophoresis separation plate 21 has a plurality ofsample wells 22 at one end and acommon buffer reservoir 23 at the other end. Afirst master electrode 25 is electrically connected to acell electrode 26 in thesample wells 22. Asecond master electrode 27 is in thecommon buffer reservoir 23.Capillary electrophoresis columns 28 are mounted in theplate 21 such that there is electrical communication between thefirst master electrode 25 by way of thecapillary electrophoresis column 28 when thesample wells 22 and thereservoir 23 are filled with electrically conductive liquid. In operation, current between the master electrodes permits electrophoresis of the sample from the sample well 22 to thereservoir 23. - Other automated capillary electrophoresis apparatuses are described in U.S. Pat. No. 6,132,582, U.S. Pat. No. 5,885,430, U.S. Pat. No. 6,118,127, U.S. Pat. No. 5,413,686.
- None of the automated analyzers described, however, are suitable for routine clinical laboratory applications. Despite their automated nature, each requires considerable manual manipulation, such as preparation and placement of individual sample and buffer vials onto the analyzer, programming for the various analytical routines, and the like. Because only one sample can undergo electrophoresis and detection during each analysis cycle of the analyzers, the number of samples per unit time, or throughput, is severely restricted compared to the needs of most routine clinical laboratory work.
- In view of the disadvantages of the conventional analyzers, the inventor of this invention has provided a new
electrophoresis separation apparatus 100 as shown in FIG. 3. Theapparatus 100 comprises amain portion 110 and a hand-heldelectrophoresis detection device 120. Since the hand-heldelectrophoresis detection device 120 is connected to themain portion 110 in a separable manner, it can be operated conveniently without the limitation of the location of themain portion 110. Thus, the detection can be performed more quickly and conveniently. - The
main portion 110 controls a detection of the sample. The hand-heldelectrophoresis detection device 120 comprises asupport 121, acartridge 122, and a plurality ofdetection columns 123. Thesupport 121 is used as a basic element of thedevice 120, and is electrically connected to themain portion 110. Thecartridge 122 is disposed in thesupport 121 in a detachable manner, and is electrically connected to thesupport 122. After detection, thecartridge 122 can be replaced quickly such that contamination between the samples is avoided. Thedetection columns 123 are disposed on thecartridge 122, and the samples are attached thereto. Thus, the samples can be moved in thedetection columns 123 to perform the detection. - In the
electrophoresis separation apparatus 100, only the basic structure of the apparatus is described. However, the detailed structure of each element is not described, such as the structure of the cartridge and the manufacturing method thereof. - In view of this, the invention provides a cartridge for an electrophoresis detection device and a manufacturing method thereof.
- Accordingly, the invention provides a cartridge for an electrophoresis detection device. The cartridge comprises a first member and a second member. The first member comprises a first concave portion. The second member comprises a second concave portion corresponding to the first concave portion. The second member is combined with the first member in a manner such that the second concave portion faces the first concave portion.
- In a preferred embodiment, the first member comprises a first protrusion and a third concave portion, formed on the first protrusion and communicating with the first concave portion. The second member comprises a second protrusion and a fourth concave portion, formed on the second protrusion and communicating with the second concave portion.
- Furthermore, the first protrusion comprises a first end at which the third concave portion communicates with the first concave portion and a second end opposite the first end, and the width of the first end exceeds that of the second end, and the second protrusion comprises a third end at which the fourth concave portion communicates with the second concave portion and a fourth end opposite the third end, the width of the third end exceeding that of the fourth end.
- In another preferred embodiment, the cartridge further comprises a capillary disposed in the first concave portion and the second concave portion in a manner such that the capillary protrudes from the first and second members.
- Furthermore, one end of the capillary, protruding from the first and second members, is tapered.
- Furthermore, the capillary comprises a plurality of through holes formed near an end protruding from the first and second members.
- In another preferred embodiment, the cartridge further comprises an electrode disposed between the first and second members in a manner such that the electrode is exposed by the first and second members.
- Furthermore, the first member comprises a fifth concave portion, and the second member comprises a sixth concave portion, and the electrode is disposed in the fifth concave portion and the sixth concave portion.
- Furthermore, the first member comprises a seventh concave portion communicating with the first concave portion and the fifth concave portion, and the second member comprises an eighth concave portion communicating with the second concave portion and the sixth concave portion.
- The invention provides a method for manufacturing a cartridge for an electrophoresis detection device. The method comprises the following steps. First, a first member, formed with an integral first concave portion, is formed. Then, a second member, formed with an integral second concave portion, is formed. Finally, the first and second members are combined in a manner such that the second concave portion faces the first concave portion.
- It is understood that the first and second members may be formed by several manufacturing methods, for example, by injection molding.
- The invention provides another method for manufacturing a cartridge for an electrophoresis detection device. The method comprises the following steps. First, a first member, formed with an integral first concave portion, is formed, and a second member, formed with an integral second concave portion, is formed. Then, a capillary is provided, and is disposed in the first concave portion. Finally, the first and second members are combined in a manner such that the second concave portion faces the first concave portion. Also, the capillary is disposed in the first concave portion and the second concave portion in a manner such that the capillary protrudes from the first and second members.
- The present invention can be more fully understood by reading the subsequent detailed description and examples with references made to the accompanying drawings, wherein:
- FIG. 1 is a schematic view of a conventional gel electrophoresis apparatus;
- FIG. 2 is a schematic view of a conventional capillary electrophoresis analyzer as described in U.S. Pat. No. 5,560,811;
- FIG. 3 is a schematic view of a conventional electrophoresis separation apparatus;
- FIG. 4a is a schematic view of a cartridge for an electrophoresis detection device as disclosed in a first embodiment of the invention;
- FIG. 4b is a combined cartridge in FIG. 4a;
- FIG. 5 is a flowchart of a method for manufacturing the cartridge in FIG. 4a;
- FIG. 6a is a schematic view of a cartridge for an electrophoresis detection device as disclosed in a second embodiment of the invention;
- FIG. 6b is a combined cartridge in FIG. 6a;
- FIG. 7a is a variant embodiment of a capillary in FIG. 6a;
- FIG. 7b is another variant embodiment of a capillary in FIG. 6a; and
- FIG. 8 is a flowchart of a method for manufacturing the cartridge in FIG. 6a.
- First Embodiment
- Referring to FIG. 4a and FIG. 4b, a cartridge for an electrophoresis detection device as disclosed in a first embodiment of the invention is shown. In this embodiment, the
cartridge 200 comprises afirst member 210, asecond member 220, and anelectrode 230. - The
first member 210 comprises a plurality of firstconcave portions 211, a plurality offirst protrusions 212, a plurality of thirdconcave portions 213, a fifthconcave portion 214, and a seventhconcave portion 215. The firstconcave portions 211 are formed around the center portion of thefirst member 210. Each of thefirst protrusions 212 is formed at the lower portion of thefirst member 210, and corresponds to the firstconcave portions 211 respectively. Each of the thirdconcave portions 213 is formed on the correspondingfirst protrusion 212, and communicates with the corresponding firstconcave portion 211 respectively. The fifthconcave portion 214 is formed at the upper portion of thefirst member 210, andelectrode 230 is disposed thereon. The seventhconcave portion 215 communicates with the firstconcave portions 211 and the fifthconcave portion 214, with a predetermined liquid disposed therein such that the liquid is in contact with theelectrode 230. - The
second member 220 comprises a plurality of secondconcave portions 221, a plurality ofsecond protrusions 222, a plurality of fourthconcave portions 223, a sixthconcave portion 224, and an eighthconcave portion 225. The secondconcave portions 221 are formed around the center portion of thesecond member 220. Each of thesecond protrusions 222 is formed at the lower portion of thesecond member 220, and corresponds to the secondconcave portions 221 respectively. Each of the fourthconcave portions 223 is formed on the correspondingsecond protrusion 222, and communicates with the corresponding secondconcave portion 221 respectively. The sixthconcave portion 224 is formed at the upper portion of thesecond member 220, andelectrode 230 is disposed thereon. The eighthconcave portion 225 communicates with the secondconcave portions 221 and the sixthconcave portion 224, with a predetermined liquid disposed therein such that the liquid is in contact with theelectrode 230. - It is understood that a plurality of channels, through which the samples flow, are formed between the first
concave portions 211, the thirdconcave portions 213 and the secondconcave portions 221, and the fourthconcave portions 223 when thesecond member 220 is combined with thefirst member 210. - It is noted that in FIG. 4a, the
first protrusion 212 comprises afirst end 212 a at which the thirdconcave portion 213 communicates with the firstconcave portion 211 and asecond end 212 b opposite thefirst end 212 a. The width W1 of thefirst end 212 a exceeds the width W2 of thesecond end 212 b. Thesecond protrusion 222 comprises athird end 222 a at which the fourthconcave portion 223 communicates with the secondconcave portion 221 and afourth end 222 b opposite thethird end 222 a. The width W3 of thethird end 222 a exceeds the width W4 of thefourth end 222 b. Thus, the tip end of the protrusion is narrower, such that thecartridge 220 is easily attached to the sample. - As shown in FIG. 4b, the
electrode 230 is disposed between thefirst member 210 and thesecond member 220 in a manner such that theelectrode 230 is exposed by thefirst member 210 and thesecond member 220. Thus, when thecartridge 200 is placed in thesupport 121 as shown in FIG. 3, theelectrode 230 can be electrically connected with thesupport 121. - While the structure of the
cartridge 200 is described as above, its manufacturing method is described hereinafter with reference to FIG. 5. - In step S11, a
first member 210 as shown in FIG. 4a is formed, and the firstconcave portions 211 are integrally formed thereon. Then, in step S12, asecond member 220 as shown in FIG. 4a is formed, whereon the secondconcave portions 221 are integrally formed. Finally, in step S13, thefirst member 210 and thesecond member 220 are combined in a manner such that the secondconcave portions 221 face the firstconcave portions 211. Also, theelectrode 230 is located between thefirst member 210 and thesecond member 220 as shown in FIG. 4b. - It is noted that the
first member 210 and thesecond member 220 may be formed by injection molding. - Furthermore, the
first member 210 and thesecond member 220 may be of polymer. - Second Embodiment
- Referring to FIG. 6a and FIG. 6b, a cartridge for an electrophoresis detection device as disclosed in a second embodiment of the invention is shown. In this embodiment, the
cartridge 300 comprises afirst member 310, asecond member 320, anelectrode 330, and a plurality ofcapillaries 340. - The
first member 310 comprises a plurality of firstconcave portions 311, a fifthconcave portion 314, and a seventhconcave portion 315. The firstconcave portions 311 are formed around the center portion of thefirst member 310. The fifthconcave portion 314 is formed at the upper portion of thefirst member 310, andelectrode 330 is disposed thereon. The seventhconcave portion 315 communicates with the firstconcave portions 311 and the fifthconcave portion 314, with a predetermined liquid disposed therein such that the liquid is in contact with theelectrode 330. - The
second member 320 comprises a plurality of secondconcave portions 321, a sixthconcave portion 324, and an eighthconcave portion 325. The secondconcave portions 321 are formed around the center portion of thesecond member 320. The sixthconcave portion 324 is formed at the upper portion of thesecond member 320, andelectrode 330 is disposed thereon. The eighthconcave portion 325 communicates with the secondconcave portions 321 and the sixthconcave portion 324, with a predetermined liquid disposed therein such that the liquid is in contact with theelectrode 330. - As shown in FIG. 6b, the
electrode 330 is disposed between thefirst member 310 and thesecond member 320 in a manner such that theelectrode 330 is exposed by thefirst member 310 and thesecond member 320. Thus, when thecartridge 300 is placed in thesupport 121 as shown in FIG. 3, theelectrode 330 is electrically connected with thesupport 121. - The
capillaries 340 are used as the channels of thecartridge 300; that is, the attached samples flow therethrough. Each of thecapillaries 340 is disposed in the firstconcave portion 311 and the secondconcave portion 321 in a manner such that it protrudes from thefirst member 310 and thesecond member 320. - In addition, the structure of the capillary340 can be changed to increase the volume of the attached sample. For example, as shown in FIG. 7a, one end, protruding from the
first member 310 and thesecond member 320, of the capillary 340 a forms a taperedportion 341. Thus, the area of the tip end of the capillary 340 a is increased such that the volume of the attached sample can be increased. Alternatively, as shown in FIG. 7b, thecartridge 340 b is formed with a plurality of throughholes 342 near the end protruding from thefirst member 310 and thesecond member 320. - While the structure of the
cartridge 300 is described above, its manufacturing method is described hereinafter with reference to FIG. 8. - In step S21, a
first member 310 as shown in FIG. 6a is formed, with the firstconcave portions 311 integrally formed thereon. Then, in step S22, asecond member 320 as shown in FIG. 6a is formed, with the secondconcave portions 321 integrally formed thereon. Instep 23, a plurality ofcapillaries 340 as shown in FIG. 6a are provided in the firstconcave portions 321. Finally, in step S24, thefirst member 310 and thesecond member 320 are combined in a manner such that the secondconcave portions 321 face the firstconcave portions 311. Also, theelectrode 330 and thecapillaries 340 are located between thefirst member 310 and thesecond member 320 as shown in FIG. 6b. - While the invention has been described by way of example and in terms of the preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiments. To the contrary, it is intended to cover various modifications and similar arrangements (as would be apparent to those skilled in the art). Therefore, the scope of the appended claims should be accorded the broadest interpretation so as to encompass all such modifications and similar arrangements.
Claims (18)
1. A cartridge for an electrophoresis detection device, comprising:
a first member comprising a first concave portion; and
a second member comprising a second concave portion corresponding to the first concave portion, wherein the second member is combined with the first member in a manner such that the second concave portion faces the first concave portion.
2. The cartridge as claimed in claim 1 , wherein the first member comprises a first protrusion and a third concave portion, formed on the first protrusion and communicating with the first concave portion, and the second member comprises a second protrusion and a fourth concave portion, formed on the second protrusion and communicating with the second concave portion.
3. The cartridge as claimed in claim 2 , wherein the first protrusion comprises a first end at which the third concave portion communicates with the first concave portion and a second end opposite the first end, and the width of the first end exceeds that of the second end, and the second protrusion comprises a third end at which the fourth concave portion communicates with the second concave portion and a fourth end opposite the third end, the width of the third end exceeding that of the fourth end.
4. The cartridge as claimed in claim 1 , further comprising:
a capillary disposed in the first concave portion and the second concave portion in a manner such that the capillary protrudes from the first and second members.
5. The cartridge as claimed in claim 4 , wherein one end of the capillary, protruding from the first and second members, is tapered.
6. The cartridge as claimed in claim 4 , wherein the capillary is formed with a plurality of through holes near an end protruding from the first and second members.
7. The cartridge as claimed in claim 1 , further comprising:
an electrode disposed between the first and second members in a manner such that the electrode is exposed by the first and second members.
8. The cartridge as claimed in claim 7 , wherein the first member comprises a fifth concave portion, and the second member comprises a sixth concave portion, and the electrode is disposed in the fifth concave portion and the sixth concave portion.
9. The cartridge as claimed in claim 8 , wherein the first member comprises a seventh concave portion communicating with the first concave portion and the fifth concave portion, and the second member comprises an eighth concave portion communicating with the second concave portion and the sixth concave portion.
10. A method for manufacturing a cartridge for an electrophoresis detection device, comprising:
forming a first member formed with an integral first concave portion;
forming a second member formed with an integral second concave portion; and
combining the first and second members in a manner such that the second concave portion faces the first concave portion.
11. The method as claimed in claim 10 , wherein the first member is formed with an integral first protrusion and a third concave portion, located on the first protrusion and communicating with the first concave portion, and the second member formed with an integral second protrusion and a fourth concave portion, located on the second protrusion and communicating with the second concave portion.
12. The method as claimed in claim 10 , further comprising providing an electrode located between the first and second members.
13. The method as claimed in claim 10 , wherein the first member is formed by injection molding.
14. The method as claimed in claim 10 , wherein the second member is formed by injection molding.
15. A method for manufacturing a cartridge for an electrophoresis detection device, comprising:
forming a first member formed with an integral first concave portion;
forming a second member formed with an integral second concave portion;
providing a capillary;
disposing the capillary in the first concave portion; and
combining the first and second members in a manner such that the second concave portion faces the first concave portion, wherein the capillary is disposed in the first concave portion and the second concave portion in a manner such that the capillary protrudes from the first and second members.
16. The method as claimed in claim 15 , further comprising:
providing an electrode between the first and second members.
17 The method as claimed in claim 15 , wherein the first member is formed by injection molding.
18. The method as claimed in claim 15 , wherein the second member is formed by injection molding.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW091120569 | 2002-09-10 | ||
TW091120569A TWI247109B (en) | 2002-09-10 | 2002-09-10 | Cartridge for electrophoresis detection device and manufacturing method thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
US20040045825A1 true US20040045825A1 (en) | 2004-03-11 |
Family
ID=31989710
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/435,587 Abandoned US20040045825A1 (en) | 2002-09-10 | 2003-05-12 | Cartridge for electrophoresis detection device and manufacturing method thereof |
Country Status (2)
Country | Link |
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US (1) | US20040045825A1 (en) |
TW (1) | TWI247109B (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105664721A (en) * | 2016-03-23 | 2016-06-15 | 内蒙古天一环境技术有限公司 | Flat-plate permeable membrane element with columnar dielectrophoresis electrodes |
JP2016535280A (en) * | 2013-09-27 | 2016-11-10 | プロテインシンプル | Apparatus, system and method for capillary electrophoresis |
Citations (4)
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US5968331A (en) * | 1994-11-11 | 1999-10-19 | Hitachi, Ltd. | Sample holding device for electrophoresis apparatus and sample injection method |
US20020061260A1 (en) * | 1999-07-15 | 2002-05-23 | Dieter Husar | Device for handling liquid samples and a process for the manufacture of the device, and a system for handling liquid samples |
US6440600B1 (en) * | 1997-05-08 | 2002-08-27 | Michael E. Starzak | Apparatus and method for generating electrical power from fluid flow through charged pores |
US20020123073A1 (en) * | 2001-01-26 | 2002-09-05 | Varouj Amirkhanian | Multi-channel bio-separation cartridge |
-
2002
- 2002-09-10 TW TW091120569A patent/TWI247109B/en not_active IP Right Cessation
-
2003
- 2003-05-12 US US10/435,587 patent/US20040045825A1/en not_active Abandoned
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5968331A (en) * | 1994-11-11 | 1999-10-19 | Hitachi, Ltd. | Sample holding device for electrophoresis apparatus and sample injection method |
US6440600B1 (en) * | 1997-05-08 | 2002-08-27 | Michael E. Starzak | Apparatus and method for generating electrical power from fluid flow through charged pores |
US20020061260A1 (en) * | 1999-07-15 | 2002-05-23 | Dieter Husar | Device for handling liquid samples and a process for the manufacture of the device, and a system for handling liquid samples |
US20020123073A1 (en) * | 2001-01-26 | 2002-09-05 | Varouj Amirkhanian | Multi-channel bio-separation cartridge |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016535280A (en) * | 2013-09-27 | 2016-11-10 | プロテインシンプル | Apparatus, system and method for capillary electrophoresis |
EP3049169A4 (en) * | 2013-09-27 | 2017-05-10 | ProteinSimple | Apparatus, systems, and methods for capillary electrophoresis |
US11933759B2 (en) | 2013-09-27 | 2024-03-19 | ProteinSimple | Apparatus, systems, and methods for capillary electrophoresis |
CN105664721A (en) * | 2016-03-23 | 2016-06-15 | 内蒙古天一环境技术有限公司 | Flat-plate permeable membrane element with columnar dielectrophoresis electrodes |
Also Published As
Publication number | Publication date |
---|---|
TWI247109B (en) | 2006-01-11 |
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