EP2353717A1 - Microtiter plate - Google Patents
Microtiter plate Download PDFInfo
- Publication number
- EP2353717A1 EP2353717A1 EP11000810A EP11000810A EP2353717A1 EP 2353717 A1 EP2353717 A1 EP 2353717A1 EP 11000810 A EP11000810 A EP 11000810A EP 11000810 A EP11000810 A EP 11000810A EP 2353717 A1 EP2353717 A1 EP 2353717A1
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- EP
- European Patent Office
- Prior art keywords
- plate
- microtiter plate
- microtiter
- vessels
- plastic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50855—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using modular assemblies of strips or of individual wells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/023—Adapting objects or devices to another adapted for different sizes of tubes, tips or container
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/028—Modular arrangements
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
Definitions
- the invention relates to a microtiter plate having a plurality of wells in a plate having at least one line of weakness, which divides the plate into different segments.
- Microtiter plates are used for a variety of microbiological, molecular biological, cell biological and immunological operations.
- microtiter plates are used for the PCR or the cultivation of microorganisms or cells.
- Microplates are only partially exploited in many laboratories. This is based in particular on the fact that the reagents used are expensive and the smallest possible number of reactions is carried out. Common applications, including qualitative PCR, often only require up to a maximum of 24 reactions. As a result, in a 96-well microtiter plate, only a portion of the vessels are used and the microtiter plate is disposed of with a portion of unused vessels.
- a microtiter plate which has a frame-like base and a plurality of vascular strips.
- the vascular strips are removable from the base and individual vessels are separable from each strip.
- the vessels are connected to each other by cross-sectionally T-shaped connecting elements which hold the vessels in a plane, rectilinear row, when the vascular strips are either held in or removed from the base.
- the T-shaped fasteners are easily destructible to allow easy separation of individual vessels. This construction is expensive. In addition, it is also expansive when it is equipped with only a few vascular strips, since the base is not adaptable to the number of vessels required.
- Corning commercializes 96-well microtiter plates under the trade designation "Thermowell® 96 Well Polypropylene PCR Microplate".
- the wells are arranged in vessels that protrude beyond the bottom of the plate and slightly beyond the top of the plate.
- the skirt equally protrudes beyond the bottom and top of the plate.
- Plate, jars and apron are made in one piece from polypropylene.
- a microtiter plate for PCR which comprises a plurality of vessels held together by a plate in matrix arrangement.
- the plate is divided into segments of predetermined size by one or more slots extending substantially across the width of the plate.
- the slots extend through the entire wall of the panel, with adjacent segments interconnected by one or more connection areas associated with each of the slots.
- the connecting portions are formed by substantially circular portions extending across the slots, the circular portions being weakened at their periphery to facilitate detachment from the disc.
- the order Slots and connecting areas are suitable for facilitating the division of the microtiter plate into segments of a predetermined size, which then fit into a thermal cycler without interfering with each other.
- the plate can be broken by bending over the slots. Alternatively, it may be cut using a pair of scissors, a knife, a scalpel or other cutting tool. Remains of material remaining at the joint areas should be removed with a cutting tool.
- microtiter plates for the PCR are made of polypropylene, because polypropylene is neutral to DNA and has a sufficient temperature resistance.
- the known PCR plate can be dismantled only after multiple bending back and forth adjacent segments to the line of weakness and tearing apart of the segments.
- the user therefore preferably uses a pair of scissors or other cutting tools.
- sharp edges may remain at which the user can injure or cut into protective gloves of the user or can tear them, so that there is a risk of contamination for sample and / or user.
- the object of the invention is to provide a microtiter plate which facilitates targeted disassembly into different segments and does not break unintentionally during use or during transport.
- microtiter plate with the features of claim 1.
- the plastic microtiter plate according to the invention has a plurality of depressions in a plate having at least one line of weakness which divides the plate into different segments, the line of weakness comprising a weld line formed by spraying various segments of the plate together.
- the weakening line comprises a weld line.
- the weld line is created by injecting the various segments of the plate together during injection molding. If the adjacent segments of the plate are injection-molded simultaneously, separately plasticized plastic compounds are introduced into the cavities of the tool, which are assigned to the different segments. The flow fronts of the plasticized plastic masses meet and there forms the weld line.
- the injection mold When injection molding a microtiter plate having a reduced cross-section at the line of weakness, the injection mold has a reduced gap in the region of the line of weakness. In the gap, the flow fronts slow down, so that they meet very evenly and in a defined position.
- the line of weakness can be defined solely by the weld line. In the weld line, the strength of the plate is reduced so that it is well breakable along the line of weakness.
- the plate can be produced by the coinjection process, whereby different segments are injected simultaneously. Different segments of the plate can also be sprayed one after the other using transfer technology, turning or shifting technology or core retraction technique. In this case, at least one segment is first sprayed and at least one further segment is injection-molded onto the sprue produced in this way in a further injection molding step. During injection molding, the flow front of the plasticized plastic mass hits the edge of the already solidified Plastic compound and melts this. This also creates a weakened weld line, which favors a clean breakage of the plate.
- the different segments can be injected from the same plastic. Further, they may be molded from the same plastic, wherein the material of the different segments may have the same color or different colors. Furthermore, the different segments can be injected from different plastics. By using different plastics for the different segments of the plate, the fracture behavior can be favorably influenced.
- microtiter plate with the features of claim 2.
- the plastic microtiter plate according to the invention has a plurality of depressions in a plate which has at least one line of weakness which divides the plate into different segments, wherein the plate is made of an amorphous plastic or of a fiber-reinforced, semi-crystalline plastic.
- the polypropylene used for conventional PCR plates has a relatively tough behavior. It has namely a relatively low stiffness and thus a relatively low modulus of elasticity (Young's modulus) and a relatively high elongation at break, so that it can not be broken in principle. This also applies to other semi-crystalline materials.
- amorphous materials are used, which are basically glassy and highly transparent. Amorphous materials have a relatively high modulus of elasticity and a relatively low elongation at break. Therefore, amorphous materials are relatively stiff, making them difficult to bend. Also, they tend to a sudden, uncontrolled break. In itself, therefore, they do not seem suitable for the Production of a microtiter plate, which should be separable into defined segments.
- a plate made of an amorphous plastic along a line of weakness is good breakable, on which the plate has a reduced wall thickness and / or one or more holes and / or a weld line.
- This can also be achieved with a plate made of fiber-reinforced, semi-crystalline plastic.
- the fiber-reinforced, partially crystalline plastic has an increased modulus of elasticity and a reduced elongation at break compared to the semi-crystalline plastic. In combination with the weakening line, this also leads to a good fracture behavior.
- microtiter plate with the features of claim 3.
- the plastic microtiter plate according to the invention has a multiplicity of depressions in a plate which has at least one line of weakness which divides the plate into different segments, the line of weakness comprising a groove on the underside of the plate.
- a good breaking behavior is achieved by the line of weakness with the groove on the underside of the plate.
- the plate breaks due to the notch effect on the groove, without a multiple back and forth bending is required. This results in a clean break.
- the arrangement of the groove on the underside favors the formation of the wells in vessels, which protrude from the underside of the plate.
- the vessels adjacent to the line of weakness are swung apart and do not disturb the breaking of the plate.
- this arrangement avoids that the vessels adjacent to the line of weakness are damaged by colliding forces when the plate is broken.
- the groove expands at least in sections towards the underside of the plate. This is manufacturing technology advantageous because it favors the demolding of the molding.
- the groove is V-shaped, U-shaped or trapezoidal. Most preferably, it is V-shaped, because a line is defined by the converging, sloping walls on which the forces are concentrated when breaking and along which the break occurs.
- the good fracture behavior is characterized by the fact that the plate preferably breaks once along the line of weakness after selectively bending once around the line of weakness to an extent that significantly exceeds the amount of bending due to usual handling and transport stresses, avoiding sharp breaklines or occur only to a small extent.
- the bending angle, which leads to breakage is preferably at least 5 ° and can be up to 180 °. More preferably, the bending angle leading to breakage is at least 10 °, more preferably at least 15 °.
- the microtiter plate does not break unintentionally during handling and transport and can be easily dismantled into segments.
- a key application advantage is that the broken-apart segments are not sharp-edged.
- the weakening line comprises at least one groove in the underside and / or in the top of the plate and / or at least one hole in the plate.
- the weakening line may have a single groove or a plurality of grooves arranged one behind the other.
- the hole may be slit-shaped or circular.
- the line of weakness may have a single hole, preferably slit-shaped, or a series of holes which may be slit-shaped or circular.
- the groove or the hole have a notch effect, which promotes good fracture behavior.
- the groove is V-shaped.
- the plate next to the groove has a wall thickness of 1 to 3 mm and / or in the groove has a wall thickness of 0.1 to 1 mm. Further preferably, the plate next to the groove has a wall thickness of 1.5 to 2.5 mm and / or in the groove has a wall thickness of 0.2 to 0.4 mm. Further preferably, the plate in the groove has a wall thickness of 0.25 to 0.35 mm, preferably of about 0.3 mm.
- the weakening line extends parallel to the narrow sides and / or the longitudinal sides of the plate.
- the line of weakness extends only parallel to the narrow sides or exclusively parallel to the longitudinal sides of the plate.
- the weakening lines terminate in edges of the plate and / or in recesses in the edges of the plate. This facilitates the breaking of the plate into different segments.
- the plate is made of a plastic having an E-modulus of at least 1500 N / mm 2 . According to a further embodiment, the plate is made of a plastic having an E-modulus of at least 2000 N / mm 2 .
- the plate is made of polycarbonate (PC), cyclo-olefin copolymer (COC), cyclo-olefin polymer (COP), polystyrene (PS) or polymethylmethacrylate (PMMA) or fiber-reinforced polypropylene (PP) or polyethylene (PE ) or other polyolefin.
- PC polycarbonate
- COC cyclo-olefin copolymer
- COP cyclo-olefin polymer
- PS polystyrene
- PMMA polymethylmethacrylate
- PP polymethylmethacrylate
- PE polyethylene
- the plate is made of a polycarbonate having an elongation at break of at most 100 to 120%.
- the plate is made of a reinforced by long glass fibers semi-crystalline plastic.
- a semi-crystalline plastic reinforced by long glass fibers the fibers lay parallel to the weld line when spraying different segments together so that no reinforcement occurs in the weld line.
- the material in the weld line fails at the interface between the fiber reinforced area and the adjacent fiber free zone.
- a reinforcement of a partially crystalline plastic with short glass fibers leads to an increased rigidity of the plate.
- the short glass fibers also reinforced the board beyond the weld line, such that the board is only breakable under more severe deformation than when reinforcing the semi-crystalline material with long glass fibers.
- the depressions can have different shapes. According to one embodiment, they are pot-shaped (cylindrical) and / or cup-shaped (for example spherical shell-shaped) and / or conical (conical). You can also have different shapes in different sections, for example, in an upper area cylindrical and conical below and cup-shaped at the bottom.
- the microtiter plate in different positions wells of different shape and / or in different segments wells of different shapes or exclusively wells with the same shape.
- the variation of the shape of the wells can thus refer to wells on different positions of the same microtiter plate, on wells in different segments of the microtiter plate or on different microtiter plates. Preference is given to a microtiter plate in which the entirety of the wells have only a single matching shape.
- the invention includes possible embodiments in which the surface of the microtiter plate varies. For example, over the plate protruding edges of the wells may be different. This different expression can refer to individual positions of the same microtiter plate, to different segments of the same microtiter plate or to different microtiter plates. However, a microtiter plate is preferred in which the surface is uniform over all segments.
- the microtiter plate has 96 wells or a multiple of 96 wells (eg 384 or 1536 wells). According to another embodiment, the microtiter plate fulfills one or more of the above-mentioned standards ANSI / SBS.
- the microtiter plate has one, two or three lines of weakness, so that it can be broken into two, three or four segments. The resulting segments are easy to handle.
- the microtiter plate can also be provided with more lines of weakness. In particular, it can be provided with lines of weakness between all adjacent columns of depressions or between all adjacent rows of depressions, so that strips comprising one column or one row of juxtaposed depressions can be separated off. But it can also be present between all adjacent columns and all adjacent rows lines of weakness, so that segments with an arbitrary number of wells can be separated. In particular, segments are separable comprising only a single recess.
- the depressions may be formed in a solid plate.
- the recesses are arranged in vessels, which are connected to the plate. This favors wells with a relatively large volume. Furthermore, it can be ensured by a suitable choice of the materials of the plate and the vessels that on the one hand, the plate has the required stability and is breakable and on the other hand comes in the wells filled sample liquid with a suitable plastic in contact. In particular, this makes it possible to carry out microtiter plates for the PCR in such a way that the plate is relatively stiff and the vessels consist of a plastic suitable for the PCR. In principle, it is also possible that the plate and the vessels are made of the same material, wherein the vessels and the plate may have the same or different colors.
- the vessels may be designed so that they do not protrude beyond the underside or the top of the plate.
- the vessels protrude from the underside and / or the top of the plate. This favors vessels with a relatively large filling volume.
- microtiter plates in which the vessels project from the underside of the plate are particularly well suited for use in PCR thermal cyclers, since the heat exchange can take place directly between the plate of the thermocycler and the walls of the vessels. If the vessels project over the top of the plate, this is advantageous for sealingly attaching a cover sheet directly to the upper edges of the vessels.
- the plate and the vessels are integrally connected to each other.
- the plate and the vessels can be integrally connected to each other in particular by injection molding.
- the plate has a plurality of holes and the vessels are integrally connected by injection molding on the edges of the holes with the plate.
- the plate consists of a first plastic and the vessels consist of a different from the first plastic second plastic. According to another embodiment, plate and vessels consist of the same plastic.
- a microtiter plate in which the vessels are integrally connected to the plate by injection molding on the edges of the holes and the vessels and the plate have different plastics is in EP 1 161 994 B2 described.
- the plate is made of a relatively rigid plastic.
- plastic for the plate is eg PC and as plastic for the vessels for use in the PCR is eg PP and for use in the oxygen supply
- silicone can be used for samples.
- This microtiter plate can be made very dimensionally stable and with very thin-walled vessels for good heat transfer or oxygen supply of samples.
- a microtiter plate in which the plate has a plurality of holes and vessels of the same plastic as the plate are integrally connected by injection to the edges of the holes in the plate is shown in EP 1 346 772 A2 described.
- This microtiter plate has the advantage of high dimensional stability and stability.
- the plate and the vessels may be made of PP, for example. Plastics of the plate and the vessels may have the same color or different colors.
- the plate according to the invention can according to the plate according to EP 1 161 994 B2 / US 2001051112 A1 or EP 1 346 772 A2 / US 2003 180 192 A1 executed or manufactured.
- vessels are connected to the plate by clipping, screwing, gluing, welding, sealing or otherwise solvable or non-detachable.
- the plate has segments which consist of different plastics and / or have different colors.
- the invention relates to a method for manufacturing a microtiter plate made of plastic with a plurality of wells in a plate having at least one line of weakness, which divides the plate into different segments, wherein the different segments of the plate are sprayed together along the line of weakness.
- the method is a coinjection method, a conversion method, a rotation or displacement method or a core withdrawal method.
- top and bottom refer to an orientation of the microtiter plate, wherein the wells are arranged with the openings above and with its closed end at the bottom.
- matching elements have the same reference numerals.
- the description of the matching elements is valid for all embodiments having them.
- a microtiter plate 1.1 comprises a plate 2.1 and a plurality of vessels 3.1. In the eight columns and in twelve rows, a total of 96 vessels 3.1 are arranged like a matrix. In the vessels 3.1 depressions 4.1 are arranged.
- the plate 2.1 is substantially rectangular with two parallel longitudinal sides 5.1, 5.2 and two parallel narrow sides 6.1, 6.2.
- the corners between adjacent longitudinal sides 5 and narrow sides 6 may have chamfers 7.1 to 7.4.
- centering holes 8.1 to 8.4 are arranged.
- the plate has 2.1 printed on the top 9, lasered or raised numbers 1 to 12, which mark the columns of the vessels 3.1.
- the plate has 2.1 printed on the top 9, lasered or raised letters A to H, which identify the rows of vessels 3.1.
- the plate 2.1 has in the bottom 11 between the third and fourth column, the sixth and seventh column and the ninth and tenth column each have a V-shaped groove 12.1, 12.2, 12.3.
- the V-shaped grooves 12.1, 12.2, 12.3 have a to the bottom 11 of the plate 2.1 expanding towards cross section.
- the wall thickness of the plate 2.1 next to the grooves 12.1, 12.2, 12.3 is 1 mm to 3 mm, preferably about 2 mm.
- the residual wall thickness of the plate is 0.2 mm to 0.4 mm, preferably about 0.3 mm.
- the grooves 12.1, 12.2, 12.3 terminate in slot-shaped recesses 13.11, 13.12, 13.21, 13.22, 13.31, 13.32, extending in the direction of the grooves 12.1, 12.2, 12.3 from the longitudinal sides 5.1, 5.2 of the plate 2.1 to the outer rows A. and H of the vessels 3.1 extend.
- the vessels 3.1 are molded onto the edges of the holes 4. They have an over the top 9 of the plate 2.1 slightly projecting, annular upper edge 14 and a projecting beyond the bottom 11 of the plate 2.1, conical portion 15 and a cup-shaped portion 16 at the bottom. At the top 9 of the plate 2.1 and at the bottom 11 of the plate 2.1, the vessels 3.1 each have a projection 17.1, 17.2 or radial projection, which supports the vessels on the top 9 and the bottom 11 of the plate 2.1.
- the plate 2.1 is injection-molded, for example, made of polycarbonate.
- the vessels 3.1 are injection-molded, for example, from polypropylene or from a silicone.
- the microtiter plate 1.1 is preferably produced in a multi-component injection molding process. It can in particular the characteristics of the plate and the vessels according to EP 1 161 994 B2 or US2001051112 A1 and prepared according to the method described in the aforementioned publications. The relevant statements in the cited references are incorporated by reference in the present application. Alternatively, the microtiter plate 1.1, the characteristics of the plate and the vessels according to EP 1 346 772 A2 or US2003180192 A1 and prepared according to the method described therein. The relevant embodiments of the cited references are incorporated by reference in the present application.
- the plate 1.2 according to Fig. 2 differs from the above-described characterized in that the lines of weakness 20.12, 20.22, 20.32 instead of the V-grooves 12.1, 12.2, 12.3 each have a series of rectangular slots 21.1, 21.2, 21.3. Furthermore, the weakening lines 20.12, 20.22, 20.32 each comprise a weld line 19.12, 19.22, 19:32. The weld line 19.12, 19.22, 19.32 in combination with the plastic used for the plate 2.2 and the notch effect due to the slots 21.1, 22.2, 21.3 also leads to a haptic pleasing fracture behavior and a smooth, supple fracture surface, if individual segments 18.1 accordingly Fig. 1 f) with respect to the remainder 18.2, 18.3, 18.4 of the plate 2.2 are folded up.
- the microtiter plate 1.3 of Fig. 3 differs from the microtiter plate 1.1 in that in the various segments vessels 3.1, 3.2, 3.3, 3.4 different shape with corresponding recesses 4.1, 4.2, 4.3, 4.4 are arranged, the shape of the recesses 4.1, 4.2, 4.3, 4.4 in the respective segment 18.1, 18.2, 18.3, 18.4 are each uniform.
- the left segment 18.1 has vessels 3.1 whose shape corresponds to the shape of the vessels 3.1 of the microtiter plate 1.1.
- the vessels 3.2 have a circular cylindrical shape.
- the vessels 3.3 have a cup shape.
- the vessels 3.4 again have a cylindrical shape, these cylinders being less high than in the vessels of the segment 18.2.
- the height of these vessels 3.4 is only about one third of the height of the vessels 3.2.
- the vessels 3.1, 3.2, 3.3, 3.4 of different shapes can be used for various investigations, in which vessels with different volumes or with different vessel shapes are useful.
- microtiter plate 1.3 Due to the lines of weakness 20.11, 20.21, 20.31 and the microtiter plate 1.3 in the same manner as the microtiter plate 1.1 can be divided into segments.
Abstract
Description
Die Erfindung betrifft eine Mikrotiterplatte mit einer Vielzahl Vertiefungen in einer Platte, die mindestens eine Schwächungslinie aufweist, welche die Platte in verschiedene Segmente unterteilt.The invention relates to a microtiter plate having a plurality of wells in a plate having at least one line of weakness, which divides the plate into different segments.
Mikrotiterplatten werden für die unterschiedlichsten mikrobiologischen, molekularbiologischen, zellbiologischen und immunologischen Arbeitsgänge verwendet. Insbesondere finden Mikrotiterplatten Anwendung für die PCR oder die Züchtung von Mikroorganismen oder Zellen.Microtiter plates are used for a variety of microbiological, molecular biological, cell biological and immunological operations. In particular, microtiter plates are used for the PCR or the cultivation of microorganisms or cells.
Von der ANSI wurden auf Empfehlung der Society of Biomolecular Screening (SBS) Standards für Mikrotiterplatten veröffentlicht, die insbesondere die Abmessungen und Positionen der Vertiefungen bei Mikrotiterplatten mit 96, 384 und 1536 Vertiefungen betreffen. Hierbei handelt es sich um die Normen ANSI/SBS 1 bis 4 - 2004 und die in Vorbereitung befindliche Norm SBS-5.Standards for microtiter plates have been published by the ANSI on the recommendation of the Society of Biomolecular Screening (SBS), in particular concerning the dimensions and positions of the wells in 96, 384 and 1536 well microtiter plates. These are the ANSI / SBS 1 to 4 - 2004 standards and the upcoming SBS-5 standard.
Mikrotiterplatten werden in vielen Laboren nur teilweise ausgenutzt. Dies beruht insbesondere darauf, dass die eingesetzten Reagenzien teuer sind und eine möglichst kleine Anzahl an Reaktionen durchgeführt wird. Häufige Anwendungen, zu denen die qualitative PCR gehört, benötigen oftmals nur bis zu maximal 24 Reaktionen. Infolgedessen wird bei einer Mikrotiterplatte mit 96 Gefäßen nur ein Teil der Gefäße benutzt und die Mikrotiterplatte mit einem Anteil unbenutzter Gefäße entsorgt.Microplates are only partially exploited in many laboratories. This is based in particular on the fact that the reagents used are expensive and the smallest possible number of reactions is carried out. Common applications, including qualitative PCR, often only require up to a maximum of 24 reactions. As a result, in a 96-well microtiter plate, only a portion of the vessels are used and the microtiter plate is disposed of with a portion of unused vessels.
Aus der
Die Firma Corning kommerzialisiert unter der Handelsbezeichnung "Thermowell® 96 Well Polypropylene PCR Microplate" mit einer seitlichen Schürze versehene Mikrotiterplatten mit 96 Vertiefungen. Die Vertiefungen sind in Gefäßen angeordnet, die über die Unterseite der Platte und geringfügig über die Oberseite der Platte hinaus stehen. Die Schürze steht gleichermaßen über die Unterseite und die Oberseite der Platte hinaus. Platte, Gefäße und Schürze sind einteilig aus Polypropylen hergestellt. In der Oberseite der Platte und in dem von der Platte hochstehenden Teil der Schürze befindet sich jeweils nach der dritten, sechsten und neunten Reihe der Vertiefungen eine Einkerbung, die zum Ansetzen eines Schneidwerkzeuges dient, um die Platte zu teilen. Ein Abbrechen einzelner Segmente ist bei dieser Mikrotiterplatte nicht praktikabel.Corning commercializes 96-well microtiter plates under the trade designation "Thermowell® 96 Well Polypropylene PCR Microplate". The wells are arranged in vessels that protrude beyond the bottom of the plate and slightly beyond the top of the plate. The skirt equally protrudes beyond the bottom and top of the plate. Plate, jars and apron are made in one piece from polypropylene. In the top of the plate and in the part of the skirt which is upstanding from the plate, there is a notch after each of the third, sixth and ninth rows of depressions, which serves to apply a cutting tool to divide the plate. Canceling individual segments is not practical with this microtiter plate.
Aus der
Derartige Mikrotiterplatten für die PCR werden aus Polypropylen hergestellt, weil Polypropylen gegenüber DNA neutral ist und eine hinreichende Temperaturbeständigkeit aufweist.Such microtiter plates for the PCR are made of polypropylene, because polypropylene is neutral to DNA and has a sufficient temperature resistance.
Die bekannte PCR-Platte ist nur nach mehrfachem Hin- und Herbiegen benachbarter Segmente um die Schwächungslinie und Auseinanderreißen der Segmente zerlegbar. Der Anwender nimmt deshalb bevorzugt eine Schere oder ein anderes Schneidwerkzeug zur Hilfe. Außerdem können nach dem Zerlegen scharfe Trennkanten verbleiben, an denen sich der Anwender verletzen kann oder die in Schutzhandschuhe des Anwenders einschneiden oder diese einreißen können, so dass sich ein Kontaminationsrisiko für Probe und/oder Anwender ergibt.The known PCR plate can be dismantled only after multiple bending back and forth adjacent segments to the line of weakness and tearing apart of the segments. The user therefore preferably uses a pair of scissors or other cutting tools. In addition, after disassembly sharp edges may remain at which the user can injure or cut into protective gloves of the user or can tear them, so that there is a risk of contamination for sample and / or user.
Davon ausgehend liegt der Erfindung die Aufgabe zugrunde, eine Mikrotiterplatte zur Verfügung zu stellen, die ein gezieltes Zerlegen in verschiedene Segmente erleichtert und nicht ungewollt bei der Anwendung oder beim Transport zerbricht.Based on this, the object of the invention is to provide a microtiter plate which facilitates targeted disassembly into different segments and does not break unintentionally during use or during transport.
Die Aufgabe wird durch eine Mikrotiterplatte mit den Merkmalen des Anspruches 1 gelöst.The object is achieved by a microtiter plate with the features of claim 1.
Die erfindungsgemäße Mikrotiterplatte aus Kunststoff hat eine Vielzahl Vertiefungen in einer Platte, die mindestens eine Schwächungslinie aufweist, welche die Platte in verschiedene Segmente unterteilt, wobei die Schwächungslinie eine Bindenaht umfasst, die durch Aneinanderspritzen verschiedener Segmente der Platte gebildet ist.The plastic microtiter plate according to the invention has a plurality of depressions in a plate having at least one line of weakness which divides the plate into different segments, the line of weakness comprising a weld line formed by spraying various segments of the plate together.
Bei der erfindungsgemäßen Mikrotiterplatte wird ein gutes Bruchverhalten dadurch erreicht, dass die Schwächungslinie eine Bindenaht umfasst. Die Bindenaht wird dadurch erzeugt, dass die verschiedenen Segmente der Platte beim Spritzgießen aneinandergespritzt werden. Falls die benachbarten Segmente der Platte gleichzeitig spritzgegossen werden, werden in die Hohlräume des Werkzeuges, die den verschiedenen Segmenten zugeordnet sind, jeweils gesondert plastifizierte Kunststoffmassen eingeleitet. Die Fließfronten der plastifizierten Kunststoffmassen treffen aufeinander und dort bildet sich die Bindenaht. Beim Spritzgießen einer Mikrotiterplatte, die an der Schwächungslinie einen reduzierten Querschnitt aufweist, weist das Spritzgießwerkzeug im Bereich der Schwächungslinie einen verringerten Spalt auf. In dem Spalt verlangsamen sich die Fließfronten, so dass sie sehr gleichmäßig und in definierter Lage aufeinandertreffen. Die Schwächungslinie kann allein durch die Bindenaht definiert sein. In der Bindenaht ist die Festigkeit der Platte vermindert, so dass sie entlang der Schwächungslinie gut brechbar ist.In the microtiter plate according to the invention a good fracture behavior is achieved in that the weakening line comprises a weld line. The weld line is created by injecting the various segments of the plate together during injection molding. If the adjacent segments of the plate are injection-molded simultaneously, separately plasticized plastic compounds are introduced into the cavities of the tool, which are assigned to the different segments. The flow fronts of the plasticized plastic masses meet and there forms the weld line. When injection molding a microtiter plate having a reduced cross-section at the line of weakness, the injection mold has a reduced gap in the region of the line of weakness. In the gap, the flow fronts slow down, so that they meet very evenly and in a defined position. The line of weakness can be defined solely by the weld line. In the weld line, the strength of the plate is reduced so that it is well breakable along the line of weakness.
Die Platte kann im Koinjektionsverfahren hergestellt werden, wobei verschiedene Segmente gleichzeitig gespritzt werden. Verschiedene Segmente der Platte können auch unter Anwendung der Umsetztechnik, Dreh- oder Verschiebetechnik oder Kernrückzugstechnik nacheinander gespritzt werden. Hierbei wird zunächst mindestens ein Segment gespritzt und an den so hergestellten Spritzling in einem weiteren Spritzgießschritt mindestens ein weiteres Segment angespritzt. Beim Anspritzen trifft die Fließfront der plastifizierten Kunststoffmasse auf den Rand der bereits erstarrten Kunststoffmasse und schmilzt diese an. Hierbei entsteht ebenfalls eine geschwächte Bindenaht, die einen sauberen Bruch der Platte begünstigt.The plate can be produced by the coinjection process, whereby different segments are injected simultaneously. Different segments of the plate can also be sprayed one after the other using transfer technology, turning or shifting technology or core retraction technique. In this case, at least one segment is first sprayed and at least one further segment is injection-molded onto the sprue produced in this way in a further injection molding step. During injection molding, the flow front of the plasticized plastic mass hits the edge of the already solidified Plastic compound and melts this. This also creates a weakened weld line, which favors a clean breakage of the plate.
Die verschiedenen Segmente können aus demselben Kunststoff gespritzt werden. Ferner können sie aus demselben Kunststoff gespritzt werden, wobei das Material der verschiedenen Segmente dieselbe Farbe oder unterschiedliche Farben aufweisen kann. Ferner können die verschiedenen Segmente aus verschiedenen Kunststoffen gespritzt werden. Durch Einsatz verschiedener Kunststoffe für die verschiedenen Segmente der Platte kann das Bruchverhalten vorteilhaft beeinflusst werden.The different segments can be injected from the same plastic. Further, they may be molded from the same plastic, wherein the material of the different segments may have the same color or different colors. Furthermore, the different segments can be injected from different plastics. By using different plastics for the different segments of the plate, the fracture behavior can be favorably influenced.
Ferner wird die Aufgabe durch eine Mikrotiterplatte mit den Merkmalen von Anspruch 2 gelöst.Furthermore, the object is achieved by a microtiter plate with the features of claim 2.
Die erfindungsgemäße Mikrotiterplatte aus Kunststoff hat eine Vielzahl Vertiefungen in einer Platte, die mindestens eine Schwächungslinie aufweist, welche die Platte in verschiedene Segmente unterteilt, wobei die Platte aus einem amorphen Kunststoff oder aus einem faserverstärkten, teilkristallinen Kunststoff hergestellt ist.The plastic microtiter plate according to the invention has a plurality of depressions in a plate which has at least one line of weakness which divides the plate into different segments, wherein the plate is made of an amorphous plastic or of a fiber-reinforced, semi-crystalline plastic.
Das für herkömmliche PCR-Platten eingesetzte Polypropylen weist ein relativ zähes Verhalten auf. Es hat nämlich eine verhältnismäßig niedrige Steifigkeit und damit einen verhältnismäßig niedrigen Elastizitätsmodul (E-Modul) und eine verhältnismäßig hohe Bruchdehnung, so dass es sich grundsätzlich nicht brechen lässt. Dies gilt auch für andere teilkristalline Materialien. Erfindungsgemäß kommen hingegen amorphe Materialien zum Einsatz, die grundsätzlich eher glasartig und hoch transparent sind. Amorphe Materialien weisen einen verhältnismäßig hohen E-Modul und eine verhältnismäßig geringe Bruchdehnung auf. Deshalb sind amorphe Materialien relativ steif, so dass sie sich schwer biegen lassen. Auch neigen sie zu einem plötzlichen, unkontrollierten Bruch. An sich erscheinen sie deshalb nicht geeignet für die Herstellung einer Mikrotiterplatte, die gezielt in definierte Segmente zerlegbar sein soll. Überraschenderweise hat sich jedoch gezeigt, dass eine Platte aus einem amorphen Kunststoff entlang einer Schwächungslinie gut brechbar ist, an der die Platte eine reduzierte Wandstärke und/oder ein oder mehrere Löchern und/oder eine Bindenaht aufweist. Dies kann auch mit einer Platte aus faserverstärktem, teilkristallinem Kunststoff erreicht werden. Der faserverstärkte, teilkristalline Kunststoff weist nämlich gegenüber dem teilkristallinen Kunststoff einen erhöhten E-Modul und eine verringerte Bruchdehnung auf. In Kombination mit der Schwächungslinie führt dies ebenfalls zu einem guten Bruchverhalten.The polypropylene used for conventional PCR plates has a relatively tough behavior. It has namely a relatively low stiffness and thus a relatively low modulus of elasticity (Young's modulus) and a relatively high elongation at break, so that it can not be broken in principle. This also applies to other semi-crystalline materials. In contrast, according to the invention amorphous materials are used, which are basically glassy and highly transparent. Amorphous materials have a relatively high modulus of elasticity and a relatively low elongation at break. Therefore, amorphous materials are relatively stiff, making them difficult to bend. Also, they tend to a sudden, uncontrolled break. In itself, therefore, they do not seem suitable for the Production of a microtiter plate, which should be separable into defined segments. Surprisingly, however, it has been found that a plate made of an amorphous plastic along a line of weakness is good breakable, on which the plate has a reduced wall thickness and / or one or more holes and / or a weld line. This can also be achieved with a plate made of fiber-reinforced, semi-crystalline plastic. The fiber-reinforced, partially crystalline plastic has an increased modulus of elasticity and a reduced elongation at break compared to the semi-crystalline plastic. In combination with the weakening line, this also leads to a good fracture behavior.
Ferner wird die Aufgabe durch eine Mikrotiterplatte mit den Merkmalen von Anspruch 3 gelöst.Furthermore, the object is achieved by a microtiter plate with the features of
Die erfindungsgemäß Mikrotiterplatte aus Kunststoff hat eine Vielzahl Vertiefungen in einer Platte, die mindestens eine Schwächungslinie aufweist, welche die Platte in verschiedene Segmente unterteilt, wobei die Schwächungslinie eine Nut an der Unterseite der Platte umfasst.The plastic microtiter plate according to the invention has a multiplicity of depressions in a plate which has at least one line of weakness which divides the plate into different segments, the line of weakness comprising a groove on the underside of the plate.
Bei der erfindungsgemäßen Mikrotiterplatte wird ein gutes Bruchverhalten durch die Schwächungslinie mit der Nut an der Unterseite der Platte erreicht. Beim Hochbiegen eines Segmentes um die Schwächungslinie bricht die Platte infolge der Kerbwirkung an der Nut, ohne dass ein mehrfaches Hin- und Herbiegen erforderlich ist. Dabei ergibt sich eine saubere Bruchstelle. Zudem wird durch die Anordnung der Nut an der Unterseite die Ausbildung der Vertiefungen in Gefäßen begünstigt, die von der Unterseite der Platte vorstehen. Beim Zerbrechen der Mikrotiterplatte werden die der Schwächungslinie benachbarten Gefäße auseinandergeschwenkt und stören das Zerbrechen der Platte nicht. Weiterhin wird durch diese Anordnung vermieden, dass die der Schwächunglinie benachbarten Gefäße beim Zerbrechen der Platte durch aufeinander gerichtete Kräfte beschädigt werden.In the microtiter plate according to the invention a good breaking behavior is achieved by the line of weakness with the groove on the underside of the plate. When bending a segment around the line of weakness, the plate breaks due to the notch effect on the groove, without a multiple back and forth bending is required. This results in a clean break. In addition, the arrangement of the groove on the underside favors the formation of the wells in vessels, which protrude from the underside of the plate. When breaking the microtiter plate, the vessels adjacent to the line of weakness are swung apart and do not disturb the breaking of the plate. Furthermore, this arrangement avoids that the vessels adjacent to the line of weakness are damaged by colliding forces when the plate is broken.
Gemäß einer Ausgestaltung erweitert sich die Nut zumindest abschnittsweise zur Unterseite der Platte hin. Dies ist herstellungstechnisch vorteilhaft, weil es die Entformung des Spritzlings begünstigt. Gemäß einer bevorzugten Ausgestaltung ist die Nut V-förmig, U-förmig oder trapezförmig. Besonders bevorzugt ist sie V-förmig, weil durch die aufeinander zu laufenden, schrägen Wände eine Linie definiert wird, auf die die Kräfte beim Brechen konzentriert werden und entlang derer der Bruch erfolgt.According to one embodiment, the groove expands at least in sections towards the underside of the plate. This is manufacturing technology advantageous because it favors the demolding of the molding. According to a preferred embodiment, the groove is V-shaped, U-shaped or trapezoidal. Most preferably, it is V-shaped, because a line is defined by the converging, sloping walls on which the forces are concentrated when breaking and along which the break occurs.
Bei sämtlichen Erfindungsvarianten zeichnet sich das gute Bruchverhalten dadurch aus, dass die Platte nach bevorzugt einmaligem gezielten Biegen um die Schwächungslinie in einem Ausmaß, das das Ausmaß einer Biegung aufgrund üblicher Belastungen bei Handling und Transport deutlich übersteigt, entlang der Schwächungslinie zerbricht, wobei scharfe Bruchkanten vermieden werden oder nur in geringem Maße auftreten. Der Biegewinkel, der zum Bruch führt, beträgt bevorzugt mindestens 5° und kann bis zu 180° betragen. Bevorzugter beträgt der Biegewinkel, der zum Bruch führt, mindestens 10°, noch bevorzugter mindestens 15°. Somit zerbricht die Mikrotiterplatte nicht ungewollt bei Handling und Transport und ist gezielt einfach in Segmente zerlegbar. Ein entscheidender Anwendungsvorteil ist, dass die auseinandergebrochenen Segmente nicht scharfkantig sind. Einerseits wird hierdurch das unmittelbare Verletzungsrisiko beim Zerlegen der Platten stark vermindert und andererseits wird die Gefahr reduziert, dass Handschuhe des Anwenders einreißen oder eingeschnitten werden. Sämtliche Erfindungsvarianten bewirken ein haptisch gefälligeres Bruchverhalten und begünstigen eine glatte, geschmeidige Bruchfläche. Bei Kombinationen der Erfindungsvarianten sind diese vorteilhaften Eigenschaften besonders ausgeprägt.In all variants of the present invention, the good fracture behavior is characterized by the fact that the plate preferably breaks once along the line of weakness after selectively bending once around the line of weakness to an extent that significantly exceeds the amount of bending due to usual handling and transport stresses, avoiding sharp breaklines or occur only to a small extent. The bending angle, which leads to breakage, is preferably at least 5 ° and can be up to 180 °. More preferably, the bending angle leading to breakage is at least 10 °, more preferably at least 15 °. Thus, the microtiter plate does not break unintentionally during handling and transport and can be easily dismantled into segments. A key application advantage is that the broken-apart segments are not sharp-edged. On the one hand, this greatly reduces the immediate risk of injury when disassembling the plates and, on the other hand, reduces the risk of the user's gloves being torn or cut. All variants of the invention produce a haptically more pleasing fracture behavior and favor a smooth, smooth fracture surface. In combinations of the invention variants, these advantageous properties are particularly pronounced.
Gemäß einer Ausgestaltung umfasst die Schwächungslinie mindestens eine Nut in der Unterseite und/oder in der Oberseite der Platte und/oder mindestens ein Loch in der Platte. Die Schwächungslinie kann eine einzige Nut oder mehrere hintereinander angeordnete Nuten aufweisen. Das Loch kann schlitzförmig oder kreisrund sein. Die Schwächungslinie kann ein einziges Loch aufweisen, dass vorzugsweise schlitzförmig ist, oder eine Serie von Löchern, die schlitzförmig oder kreisrund sein können. Die Nut bzw. das Loch haben eine Kerbwirkung, die ein gutes Bruchverhalten fördert. Bevorzugt ist die Nut V-förmig.According to one embodiment, the weakening line comprises at least one groove in the underside and / or in the top of the plate and / or at least one hole in the plate. The weakening line may have a single groove or a plurality of grooves arranged one behind the other. The hole may be slit-shaped or circular. The line of weakness may have a single hole, preferably slit-shaped, or a series of holes which may be slit-shaped or circular. The groove or the hole have a notch effect, which promotes good fracture behavior. Preferably, the groove is V-shaped.
Gemäß einer bevorzugten Ausgestaltung hat die Platte neben der Nut eine Wanddicke von 1 bis 3 mm und/oder in der Nut eine Wanddicke von 0,1 bis 1 mm. Weiterhin bevorzugt weist die Platte neben der Nut eine Wanddicke von 1,5 bis 2,5 mm und/oder in der Nut eine Wanddicke von 0,2 bis 0,4 mm auf. Weiterhin bevorzugt hat die Platte in der Nut eine Wanddicke von 0,25 bis 0,35 mm, vorzugsweise von etwa 0,3 mm.According to a preferred embodiment, the plate next to the groove has a wall thickness of 1 to 3 mm and / or in the groove has a wall thickness of 0.1 to 1 mm. Further preferably, the plate next to the groove has a wall thickness of 1.5 to 2.5 mm and / or in the groove has a wall thickness of 0.2 to 0.4 mm. Further preferably, the plate in the groove has a wall thickness of 0.25 to 0.35 mm, preferably of about 0.3 mm.
Gemäß einer Ausgestaltung erstreckt sich die Schwächungslinie parallel zu den Schmalseiten und/oder den Längsseiten der Platte. Vorzugsweise erstreckt sich die Schwächungslinie ausschließlich parallel zu den Schmalseiten oder ausschließlich parallel zu den Längsseiten der Platte. Dies hat den Vorteil, dass von der Mikrotiterplatte Segmente abtrennbar sind, die mit den üblichen Mehrkanalpipetten befüllbar sind. Bei Ausführung der Mehrkanalpipetten mit acht Kanälen sind nämlich die zu den Schmalseiten parallelen Spalten und bei Ausführung der Mehrkanalpipetten mit zwölf Kanälen die zu den Längsseiten parallelen Reihen der Vertiefungen befüllbar.According to one embodiment, the weakening line extends parallel to the narrow sides and / or the longitudinal sides of the plate. Preferably, the line of weakness extends only parallel to the narrow sides or exclusively parallel to the longitudinal sides of the plate. This has the advantage that can be separated from the microtiter plate segments that can be filled with the usual multi-channel pipettes. In the case of the multichannel pipettes having eight channels, the columns parallel to the narrow sides and, when the multichannel pipettes are provided with twelve channels, the rows of depressions parallel to the longitudinal sides can be filled.
Gemäß einer weiteren Ausgestaltung enden die Schwächungslinien in Rändern der Platte und/oder in Aussparungen in den Rändern der Platte. Hierdurch wird das Zerbrechen der Platte in verschiedene Segmente erleichtert. [HI]According to a further embodiment, the weakening lines terminate in edges of the plate and / or in recesses in the edges of the plate. This facilitates the breaking of the plate into different segments. [HI]
Gemäß einer bevorzugten Ausgestaltung ist die Platte aus einem Kunststoff mit einem E-Modul von mindestens 1500 N/mm2 hergestellt. Gemäß einer weiteren Ausgestaltung ist die Platte aus einem Kunststoff mit einem E-Modul von mindestens 2000 N/mm2 hergestellt.According to a preferred embodiment, the plate is made of a plastic having an E-modulus of at least 1500 N / mm 2 . According to a further embodiment, the plate is made of a plastic having an E-modulus of at least 2000 N / mm 2 .
Gemäß einer Ausgestaltung ist die Platte aus Polycarbonat (PC), Cyclo-Olefin-Copolymer (COC), Cyclo-Olefin-Polymer (COP), Polystyrol (PS) oder Polymethylmethacrylat (PMMA) oder aus faserverstärktem Polypropylen (PP) oder Polyethylen (PE) oder anderem Polyolefin hergestellt.According to one embodiment, the plate is made of polycarbonate (PC), cyclo-olefin copolymer (COC), cyclo-olefin polymer (COP), polystyrene (PS) or polymethylmethacrylate (PMMA) or fiber-reinforced polypropylene (PP) or polyethylene (PE ) or other polyolefin.
Gemäß einer bevorzugten Ausgestaltung ist die Platte aus einem Polycarbonat mit einer Bruchdehnung von höchstens 100 bis 120 % hergestellt.According to a preferred embodiment, the plate is made of a polycarbonate having an elongation at break of at most 100 to 120%.
Gemäß einer weiteren Ausgestaltung ist die Platte aus einem durch Langglasfasern verstärkten teilkristallinen Kunststoff hergestellt. Bei einem durch Langglasfasern verstärkten teilkristallinen Kunststoff legen sich die Fasern beim Aneinanderspritzen verschiedener Segmente parallel zur Bindenaht, so dass in der Bindenaht keine Verstärkung eintritt. Infolgedessen versagt das Material in der Bindenaht an der Grenzfläche zwischen dem faserverstärkten Bereich und der angrenzenden faserfreien Zone. Im Unterschied dazu führt eine Verstärkung eines teilkristallinen Kunststoffes mit Kurzglasfasern zwar zu einer erhöhten Steifigkeit der Platte. Die Kurzglasfasern verstärkten die Platte jedoch auch über die Bindenaht hinweg, so dass die Platte nur unter stärkerer Verformung brechbar ist als bei einer Verstärkung des teilkristallinen Materials durch Langglasfasern.According to a further embodiment, the plate is made of a reinforced by long glass fibers semi-crystalline plastic. In a semi-crystalline plastic reinforced by long glass fibers, the fibers lay parallel to the weld line when spraying different segments together so that no reinforcement occurs in the weld line. As a result, the material in the weld line fails at the interface between the fiber reinforced area and the adjacent fiber free zone. In contrast, although a reinforcement of a partially crystalline plastic with short glass fibers leads to an increased rigidity of the plate. However, the short glass fibers also reinforced the board beyond the weld line, such that the board is only breakable under more severe deformation than when reinforcing the semi-crystalline material with long glass fibers.
Die Vertiefungen können verschiedene Formen aufweisen. Gemäß einer Ausgestaltung sind sie topfförmig (zylindrisch) und/oder napfförmig (z.B. kugelschalenförmig) und/oder konisch (kegelförmig). Sie können auch in verschiedenen Abschnitten verschiedene Formen aufweisen, beispielsweise in einem oberen Bereich zylindrisch und darunter konisch und am unteren Ende napfförmig.The depressions can have different shapes. According to one embodiment, they are pot-shaped (cylindrical) and / or cup-shaped (for example spherical shell-shaped) and / or conical (conical). You can also have different shapes in different sections, for example, in an upper area cylindrical and conical below and cup-shaped at the bottom.
Gemäß einer weiteren Ausgestaltung weist die Mikrotiterplatte in verschiedenen Positionen Vertiefungen verschiedener Form und/oder in verschiedenen Segmenten Vertiefungen verschiedener Form oder ausschließlich Vertiefungen mit derselben Form auf. Die Variation der Form der Vertiefungen kann sich also auf Vertiefungen auf verschiedenen Positionen derselben Mikrotiterplatte, auf Vertiefungen in verschiedenen Segmenten der Mikrotiterplatte oder auf verschiedene Mikrotiterplatten beziehen. Bevorzugt wird eine Mikrotiterplatte, bei der die Gesamtheit der Vertiefungen nur eine einzige übereinstimmende Form aufweist.According to a further embodiment, the microtiter plate in different positions wells of different shape and / or in different segments wells of different shapes or exclusively wells with the same shape. The variation of the shape of the wells can thus refer to wells on different positions of the same microtiter plate, on wells in different segments of the microtiter plate or on different microtiter plates. Preference is given to a microtiter plate in which the entirety of the wells have only a single matching shape.
Ferner bezieht die Erfindung mögliche Ausgestaltungen ein, bei denen die Oberfläche der Mikrotiterplatte variiert. Beispielsweise können über die Platte herausragende Ränder der Vertiefungen unterschiedlich ausgeprägt sein. Diese unterschiedliche Ausprägung kann sich auf einzelne Positionen derselben Mikrotiterplatte, auf verschiedene Segmente derselben Mikrotiterplatte oder auf verschiedene Mikrotiterplatten beziehen. Bevorzugt wird jedoch eine Mikrotiterplatte, bei der über alle Segmente hinweg die Oberfläche einheitlich ausgeprägt ist.Furthermore, the invention includes possible embodiments in which the surface of the microtiter plate varies. For example, over the plate protruding edges of the wells may be different. This different expression can refer to individual positions of the same microtiter plate, to different segments of the same microtiter plate or to different microtiter plates. However, a microtiter plate is preferred in which the surface is uniform over all segments.
Gemäß einer weiteren Ausgestaltung weist die Mikrotiterplatte 96 Vertiefungen oder ein Vielfaches von 96 Vertiefungen (z.B. 384 oder 1536 Vertiefungen) auf. Gemäß einer weiteren Ausgestaltung erfüllt die Mikrotiterplatte eine oder mehrere der o.g. Normen ANSI/SBS.According to another embodiment, the microtiter plate has 96 wells or a multiple of 96 wells (eg 384 or 1536 wells). According to another embodiment, the microtiter plate fulfills one or more of the above-mentioned standards ANSI / SBS.
Gemäß einer weiteren Ausgestaltung weist die Mikrotiterplatte eine, zwei oder drei Schwächungslinien auf, so dass sie in zwei, drei oder vier Segmente zerbrechbar ist. Die resultierenden Segmente sind gut handhabbar. Die Mikrotiterplatte kann aber auch mit mehr Schwächungslinien versehen werden. Sie kann insbesondere zwischen sämtlichen benachbarten Spalten aus Vertiefungen oder zwischen sämtlichen benachbarten Reihen aus Vertiefungen mit Schwächungslinien versehen sein, so dass Streifen umfassend jeweils eine Spalte oder eine Reihe nebeneinander angeordneter Vertiefungen abtrennbar sind. Es können aber auch zwischen sämtlichen benachbarten Spalten und sämtlichen benachbarten Reihen Schwächungslinien vorhanden sein, so dass Segmente mit einer beliebigen Anzahl Vertiefungen abtrennbar sind. Insbesondere sind Segmente abtrennbar, die nur eine einzige Vertiefung umfassen.According to a further embodiment, the microtiter plate has one, two or three lines of weakness, so that it can be broken into two, three or four segments. The resulting segments are easy to handle. The microtiter plate can also be provided with more lines of weakness. In particular, it can be provided with lines of weakness between all adjacent columns of depressions or between all adjacent rows of depressions, so that strips comprising one column or one row of juxtaposed depressions can be separated off. But it can also be present between all adjacent columns and all adjacent rows lines of weakness, so that segments with an arbitrary number of wells can be separated. In particular, segments are separable comprising only a single recess.
Die Vertiefungen können in einer massiven Platte ausgebildet sein. Gemäß einer bevorzugten Ausgestaltung sind die Vertiefungen in Gefäßen angeordnet, die mit der Platte verbunden sind. Dies begünstigt Vertiefungen mit einem verhältnismäßig großen Volumen. Ferner kann durch geeignete Wahl der Materialien der Platte und der Gefäße sichergestellt werden, dass einerseits die Platte die erforderliche Stabilität aufweist und brechbar ist und andererseits in die Vertiefungen eingefüllte Probenflüssigkeit mit einem geeigneten Kunststoff in Kontakt kommt. Insbesondere ermöglicht dies, Mikrotiterplatten für die PCR so auszuführen, dass die Platte verhältnismäßig steif ist und die Gefäße aus einem für die PCR geeigneten Kunststoff bestehen. Grundsätzlich ist es aber auch möglich, dass die Platte und die Gefäße aus demselben Material bestehen, wobei die Gefäße und die Platte die gleiche oder unterschiedliche Farben aufweisen können.The depressions may be formed in a solid plate. According to a preferred embodiment, the recesses are arranged in vessels, which are connected to the plate. This favors wells with a relatively large volume. Furthermore, it can be ensured by a suitable choice of the materials of the plate and the vessels that on the one hand, the plate has the required stability and is breakable and on the other hand comes in the wells filled sample liquid with a suitable plastic in contact. In particular, this makes it possible to carry out microtiter plates for the PCR in such a way that the plate is relatively stiff and the vessels consist of a plastic suitable for the PCR. In principle, it is also possible that the plate and the vessels are made of the same material, wherein the vessels and the plate may have the same or different colors.
Grundsätzlich können die Gefäße so ausgeführt sein, dass sie nicht über die Unterseite bzw. die Oberseite der Platte hinaus stehen. Gemäß einer bevorzugten Ausgestaltung stehen die Gefäße von der Unterseite und/oder der Oberseite der Platte vor. Dies begünstigt Gefäße mit verhältnismäßig großem Füllvolumen. Außerdem sind Mikrotiterplatten, bei denen die Gefäße von der Unterseite der Platte vorstehen, besonders gut für den Einsatz in Thermocyclern für die PCR geeignet, da der Wärmeaustausch direkt zwischen der Platte des Thermocyclers und den Wänden der Gefäße erfolgen kann. Falls die Gefäße über die Oberseite der Platte vorstehen, ist dies vorteilhaft für die abdichtende Anbringung einer Abdeckfolie direkt an den oberen Rändern der Gefäße.In principle, the vessels may be designed so that they do not protrude beyond the underside or the top of the plate. According to a preferred embodiment, the vessels protrude from the underside and / or the top of the plate. This favors vessels with a relatively large filling volume. In addition, microtiter plates in which the vessels project from the underside of the plate are particularly well suited for use in PCR thermal cyclers, since the heat exchange can take place directly between the plate of the thermocycler and the walls of the vessels. If the vessels project over the top of the plate, this is advantageous for sealingly attaching a cover sheet directly to the upper edges of the vessels.
Gemäß einer bevorzugten Ausgestaltung sind die Platte und die Gefäße einteilig miteinander verbunden. Die Platte und die Gefäße können insbesondere durch Spritzgießen einteilig miteinander verbunden sein. Gemäß einer Ausgestaltung weist die Platte eine Vielzahl Löcher auf und sind die Gefäße durch Anspritzen an die Ränder der Löcher einteilig mit der Platte verbunden.According to a preferred embodiment, the plate and the vessels are integrally connected to each other. The plate and the vessels can be integrally connected to each other in particular by injection molding. According to one embodiment, the plate has a plurality of holes and the vessels are integrally connected by injection molding on the edges of the holes with the plate.
Gemäß einer Ausgestaltung besteht die Platte aus einem ersten Kunststoff und bestehen die Gefäße aus einem vom ersten Kunststoff verschiedenen zweiten Kunststoff. Gemäß einer anderen Ausgestaltung bestehen Platte und Gefäße aus demselben Kunststoff.According to one embodiment, the plate consists of a first plastic and the vessels consist of a different from the first plastic second plastic. According to another embodiment, plate and vessels consist of the same plastic.
Eine Mikrotiterplatte, bei der die Gefäße durch Anspritzen an die Ränder der Löcher einteilig mit der Platte verbunden sind und die Gefäße und die Platte unterschiedliche Kunststoffe aufweisen, ist in der
Eine Mikrotiterplatte, bei der die Platte eine Vielzahl Löcher aufweist und Gefäße aus demselben Kunststoff wie die Platte durch Anspritzen an die Ränder der Löcher einteilig mit der Platte verbunden sind, ist in der
Die erfindungsgemäße Platte kann entsprechend der Platte gemäß
Grundsätzlich ist es aber auch möglich, dass die Gefäße mit der Platte durch Einclipsen, Einschrauben, Kleben, Schweißen, Siegeln oder in anderer Weise lösbar oder unlösbar verbunden sind.In principle, it is also possible that the vessels are connected to the plate by clipping, screwing, gluing, welding, sealing or otherwise solvable or non-detachable.
Gemäß einer weiteren Ausgestaltung weist die Platte Segmente auf, die aus verschiedenem Kunststoff bestehen und/oder verschiedene Farben aufweisen.According to a further embodiment, the plate has segments which consist of different plastics and / or have different colors.
Die Bereitstellung verschiedener Formen der Vertiefungen und/oder Oberflächen der Mikrotiterplatte und/oder Farben der Segmente und/oder Gefäße bietet die Möglichkeit einer Kennung/Markierung von Proben. Dies ermöglicht beispielsweise für verschiedene Schritte eines diagnostischen Arbeitsablaufs optisch klare bzw. durchsichtige Gefäße für die Vorbereitung von Proben, weiße Gefäße für die Real-Time PCR und schwarze, optisch undurchlässige Gefäße für eine lichtgeschützte Lagerung zu verwenden.The provision of various shapes of the wells and / or surfaces of the microtiter plate and / or colors of the segments and / or vessels offers the possibility of identification / marking of samples. This allows, for example, for different steps of a diagnostic workflow optically clear or transparent vessels for the preparation of samples, white vessels for the real-time Use PCR and black optically opaque tubes for light protected storage.
Ferner betrifft die Erfindung ein Verfahren zum Herstellen einer Mikrotiterplatte aus Kunststoff mit einer Vielzahl Vertiefungen in einer Platte, die mindestens eine Schwächungslinie aufweist, welche die Platte in verschiedene Segmente unterteilt, wobei die verschiedenen Segmente der Platte entlang der Schwächungslinie aneinandergespritzt werden.Furthermore, the invention relates to a method for manufacturing a microtiter plate made of plastic with a plurality of wells in a plate having at least one line of weakness, which divides the plate into different segments, wherein the different segments of the plate are sprayed together along the line of weakness.
Gemäß einer Ausgestaltung ist das Verfahren ein Koinjektionsverfahren, ein Umsetzverfahren, ein Dreh- oder Verschiebeverfahren oder ein Kernrückzugsverfahren.According to one embodiment, the method is a coinjection method, a conversion method, a rotation or displacement method or a core withdrawal method.
Die Erfindung wird nachfolgend anhand der anliegenden Zeichnungen eines Ausführungsbeispiels näher erläutert. In den Zeichnungen zeigen:
- Fig. 1a bis f
- eine Mikrotiterplatte mit 96 Vertiefungen in einer Perspektivansicht schräg von oben und von der Seite (
Fig. 1a ), in einer Perspektivansicht schräg von unten und von der Seite (Fig. 1b ), in einer vergrößerten Detailansicht schräg von unten und von der Seite (Fig. 1c ), in einem vergrößerten Vertikalschnitt durch zwei benachbarte Gefäße (Fig. 1d ), in einer vergrößerten Draufsicht auf den Randbereich und zwei benachbarte Gefäße (Fig. 1e ) und beim Abbrechen eines Segments in einer Perspektivansicht schräg von oben und von der Seite (Fig. 1f ); - Fig. 2
- eine andere Mikrotiterplatte mit unterbrochene Schlitze aufweisenden Schwächungslinien in der Draufsicht;
- Fig. 3a bis c
- eine andere Mikrotiterplatte mit Vertiefungen verschiedener Form in einer Perspektivansicht schräg von unten und von der Seite (
Fig. 3a ), in einem Längsschnitt entlang der Linie A-A von Fig. 6c (Fig. 3b ) und in einer Draufsicht (Fig. 3c ).
- Fig. 1a to f
- a 96-well microtiter plate in a perspective view obliquely from above and from the side (
Fig. 1a ), in a perspective view obliquely from below and from the side (Fig. 1b ), in an enlarged detail view obliquely from below and from the side (Fig. 1c ), in an enlarged vertical section through two adjacent vessels (Fig. 1d ), in an enlarged plan view of the edge region and two adjacent vessels (Fig. 1e ) and when breaking a segment in a perspective view obliquely from above and from the side (Fig. 1f ); - Fig. 2
- another microtiter plate with broken slits having weakening lines in plan view;
- Fig. 3a to c
- another microtiter plate with wells of different shape in a perspective view obliquely from below and from the side (
Fig. 3a ), in a longitudinal section along the line AA of Fig. 6c (Fig. 3b ) and in a plan view (Fig. 3c ).
In der vorliegenden Anmeldung beziehen sich die Angaben "oben" und "unten" auf eine Ausrichtung der Mikrotiterplatte, bei der die Vertiefungen mit den Öffnungen oben und mit ihrem geschlossenen Ende unten angeordnet sind.In the present application, the terms "top" and "bottom" refer to an orientation of the microtiter plate, wherein the wells are arranged with the openings above and with its closed end at the bottom.
Bei der nachfolgenden Beschreibung verschiedener Ausführungsbeispiele haben übereinstimmende Elemente dieselben Bezugsziffern. Die Beschreibung der übereinstimmenden Elemente hat für sämtliche Ausführungsbeispiele Gültigkeit, die diese aufweisen.In the following description of various embodiments, matching elements have the same reference numerals. The description of the matching elements is valid for all embodiments having them.
Gemäß
Die Platte 2.1 ist im Wesentlichen rechteckig mit zwei parallelen Längsseiten 5.1, 5.2 und zwei parallelen Schmalseiten 6.1, 6.2. Die Ecken zwischen benachbarten Längsseiten 5 und Schmalseiten 6 können Anschrägungen 7.1 bis 7.4 aufweisen. Neben den Ecken der Platte 2.1 sind Zentrierlöcher 8.1 bis 8.4 angeordnet.The plate 2.1 is substantially rectangular with two parallel longitudinal sides 5.1, 5.2 and two parallel narrow sides 6.1, 6.2. The corners between adjacent longitudinal sides 5 and narrow sides 6 may have chamfers 7.1 to 7.4. In addition to the corners of the plate 2.1 centering holes 8.1 to 8.4 are arranged.
Neben der Längsseite 5.1 hat die Platte 2.1 auf der Oberseite 9 aufgedruckte, gelaserte oder erhabene Ziffern 1 bis 12, die die Spalten der Gefäße 3.1 kennzeichnen. Neben der Schmalseite 6.1 hat die Platte 2.1 auf der Oberseite 9 aufgedruckte, gelaserte oder erhabene Buchstaben A bis H, die die Reihen der Gefäße 3.1 kennzeichnen.In addition to the long side 5.1, the plate has 2.1 printed on the top 9, lasered or raised numbers 1 to 12, which mark the columns of the vessels 3.1. In addition to the narrow side 6.1, the plate has 2.1 printed on the top 9, lasered or raised letters A to H, which identify the rows of vessels 3.1.
In den oberen Rändern der Schmalseiten können Stufen 10.1, 10.2 angeordnet sein.In the upper edges of the narrow sides stages 10.1, 10.2 may be arranged.
Die Platte 2.1 hat in der Unterseite 11 zwischen der dritten und der vierten Spalte, der sechsten und siebten Spalte und der neunten und zehnten Spalte jeweils eine V-förmige Nut 12.1, 12.2, 12.3. Die V-förmigen Nuten 12.1, 12.2, 12.3 haben einen sich zur Unterseite 11 der Platte 2.1 hin erweiternden Querschnitt. Die Wanddicke der Platte 2.1 neben den Nuten 12.1, 12.2, 12.3 beträgt 1 mm bis 3 mm, vorzugsweise etwa 2 mm. In den Nuten 12.1, 12.2, 12.3 beträgt die Restwanddicke der Platte 0,2 mm bis 0,4 mm, vorzugsweise etwa 0,3 mm.The plate 2.1 has in the bottom 11 between the third and fourth column, the sixth and seventh column and the ninth and tenth column each have a V-shaped groove 12.1, 12.2, 12.3. The V-shaped grooves 12.1, 12.2, 12.3 have a to the bottom 11 of the plate 2.1 expanding towards cross section. The wall thickness of the plate 2.1 next to the grooves 12.1, 12.2, 12.3 is 1 mm to 3 mm, preferably about 2 mm. In the grooves 12.1, 12.2, 12.3, the residual wall thickness of the plate is 0.2 mm to 0.4 mm, preferably about 0.3 mm.
Die Nuten 12.1, 12.2, 12.3 enden in schlitzförmigen Aussparungen 13.11, 13.12, 13.21, 13.22, 13.31, 13.32, die sich in Richtung der Nuten 12.1, 12.2, 12.3 von den Längsseiten 5.1, 5.2 der Platte 2.1 aus bis zu den äußeren Reihen A und H der Gefäße 3.1 erstrecken.The grooves 12.1, 12.2, 12.3 terminate in slot-shaped recesses 13.11, 13.12, 13.21, 13.22, 13.31, 13.32, extending in the direction of the grooves 12.1, 12.2, 12.3 from the longitudinal sides 5.1, 5.2 of the plate 2.1 to the outer rows A. and H of the vessels 3.1 extend.
Die Gefäße 3.1 sind an die Ränder der Löcher 4 angespritzt. Sie haben einen über die Oberseite 9 der Platte 2.1 etwas hinausstehenden, kreisringförmigen oberen Rand 14 und einen über die Unterseite 11 der Platte 2.1 hinausstehenden, konischen Abschnitt 15 sowie einen napfförmigen Abschnitt 16 am unteren Ende. An der Oberseite 9 der Platte 2.1 und an der Unterseite 11 der Platte 2.1 haben die Gefäße 3.1 jeweils einen Überstand 17.1, 17.2 bzw. radialen Vorsprung, der die Gefäße an der Oberseite 9 und der Unterseite 11 der Platte 2.1 abstützt.The vessels 3.1 are molded onto the edges of the
Die Platte 2.1 ist beispielsweise aus Polycarbonat spritzgegossen. Die Gefäße 3.1 sind beispielsweise aus Polypropylen oder aus einem Silikon spritzgegossen. Die Mikrotiterplatte 1.1 ist vorzugsweise im Mehrkomponenten-Spritzgießverfahren hergestellt. Sie kann insbesondere die Merkmale der Platte und der Gefäße gemäß
Bei der Herstellung der Mikrotiterplatte 1.1 werden zudem die durch die V-Nuten 12.1, 12.2, 12.3 voneinander getrennten Segmente 18.1, 18.2, 18.3, 18.4 der Platte 2.1 einzeln gespritzt. Infolgedessen ergeben sich an der Basis der Nuten 12.1, 12.2, 12.3 Bindenähte 19.11, 19.21, 19.31, an denen sich die Schmelzfronten der plastifizierten Materialien treffen, die die verschiedenen Segmente 18.1, 18.2, 18.3, 18.4 der Platte 2.1 bilden. Die Bindenähte 19.11, 19.21, 19.31 bilden gemeinsam mit den V-Nuten 12.1, 12.2, 12.3 Schwächungslinien 20.11, 20.21, 20.31.In the production of the microtiter plate 1.1 also by the V-grooves 12.1, 12.2, 12.3 separated segments 18.1, 18.2, 18.3, 18.4 of the plate 2.1 are sprayed individually. As a result, at the base of the grooves 12.1, 12.2, 12.3 tie lines 19.11, 19.21, 19.31, where meet the enamel fronts of the plasticized materials that form the various segments 18.1, 18.2, 18.3, 18.4 of the plate 2.1. The weld lines 19.11, 19.21, 19.31 together with the V-grooves 12.1, 12.2, 12.3 weakening lines 20.11, 20.21, 20.31.
Aufgrund der Bindenähte 19.1, 19.2, 19.3, der Verwendung von Polycarbonat für die Platte 2.1 und der Kerbwirkung aufgrund der V-Nuten 12.1, 12.2, 12.3 kommt es beim Hochbiegen einzelner Segmente 18.1, 18.2, 18.3, 18.4 zu einem haptisch gefälligen Bruchverhalten und zu einer glatten, geschmeidigen Bruchfläche. Der Bruch tritt ein, wenn ein Segment 18.1 der Platte 2.1 entsprechend
Die Platte 1.2 gemäß
Die Mikrotiterplatte 1.3 von
Die Gefäße 3.1, 3.2, 3.3, 3.4 verschiedener Formen sind für verschiedene Untersuchungen nutzbar, in denen Gefäße mit verschiedenen Volumina bzw. mit unterschiedlichen Gefäßformen nützlich sind.The vessels 3.1, 3.2, 3.3, 3.4 of different shapes can be used for various investigations, in which vessels with different volumes or with different vessel shapes are useful.
Aufgrund der Schwächungslinien 20.11, 20.21, 20.31 ist auch die Mikrotiterplatte 1.3 in derselben Weise wie die Mikrotiterplatte 1.1 in Segmente zerlegbar.Due to the lines of weakness 20.11, 20.21, 20.31 and the microtiter plate 1.3 in the same manner as the microtiter plate 1.1 can be divided into segments.
Claims (25)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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DE201010008036 DE102010008036A1 (en) | 2010-02-05 | 2010-02-05 | microtiter plate |
Publications (2)
Publication Number | Publication Date |
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EP2353717A1 true EP2353717A1 (en) | 2011-08-10 |
EP2353717B1 EP2353717B1 (en) | 2012-12-26 |
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EP20110000810 Active EP2353717B1 (en) | 2010-02-05 | 2011-02-02 | Microtiter plate |
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CN (1) | CN102151590A (en) |
DE (1) | DE102010008036A1 (en) |
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EP2539073B1 (en) * | 2010-02-22 | 2014-12-17 | 4titude Ltd. | Multiwell strips |
WO2019178703A1 (en) | 2018-03-23 | 2019-09-26 | Ambartsoumian Gourgen | Pcr tube holder |
WO2020043687A1 (en) * | 2018-08-27 | 2020-03-05 | Sabic Global Technologies B.V. | Multi-cup arrangement for foodstuff packaging. |
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CN102943031A (en) * | 2012-10-30 | 2013-02-27 | 无锡耐思生物科技有限公司 | 96-well polymerase chain reaction tandem alignment tube structure |
CN104015306B (en) * | 2014-06-09 | 2016-08-31 | 东莞市积健生物科技有限公司 | PCR plate injection forming mold and method thereof |
US10137453B2 (en) * | 2014-12-10 | 2018-11-27 | Biotix, Inc. | Static-defeating apparatus for pipette tips |
JP2019508222A (en) * | 2015-12-22 | 2019-03-28 | スリーエム イノベイティブ プロパティズ カンパニー | Stem-well film for sample distribution |
JP6635532B1 (en) * | 2019-03-25 | 2020-01-29 | グローバルサイエンス株式会社 | Sample cup assembly |
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Also Published As
Publication number | Publication date |
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EP2353717B1 (en) | 2012-12-26 |
CN102151590A (en) | 2011-08-17 |
DE102010008036A1 (en) | 2011-08-11 |
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