EP1904653A2 - Microfluidic methods and apparatuses for sample preparation and analysis - Google Patents
Microfluidic methods and apparatuses for sample preparation and analysisInfo
- Publication number
- EP1904653A2 EP1904653A2 EP06787065A EP06787065A EP1904653A2 EP 1904653 A2 EP1904653 A2 EP 1904653A2 EP 06787065 A EP06787065 A EP 06787065A EP 06787065 A EP06787065 A EP 06787065A EP 1904653 A2 EP1904653 A2 EP 1904653A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- fluid
- microchannel
- sheathing
- carrier fluid
- targets
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0636—Focussing flows, e.g. to laminate flows
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0663—Stretching or orienting elongated molecules or particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
Abstract
Description
Claims
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PCT/US2006/027111 WO2007011622A2 (en) | 2005-07-18 | 2006-07-13 | Microfluidic methods and apparatuses for sample preparation and analysis |
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EP1904653A2 true EP1904653A2 (en) | 2008-04-02 |
EP1904653A4 EP1904653A4 (en) | 2010-04-14 |
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EP06787065A Withdrawn EP1904653A4 (en) | 2005-07-18 | 2006-07-13 | Microfluidic methods and apparatuses for sample preparation and analysis |
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---|---|---|---|---|
CA2580589C (en) * | 2006-12-19 | 2016-08-09 | Fio Corporation | Microfluidic detection system |
US20090036324A1 (en) * | 2007-07-16 | 2009-02-05 | Rong Fan | Arrays, substrates, devices, methods and systems for detecting target molecules |
EP2209549A4 (en) | 2007-10-12 | 2014-03-05 | Fio Corp | Flow focusing method and system for forming concentrated volumes of microbeads, and microbeads formed further thereto |
WO2009155704A1 (en) | 2008-06-25 | 2009-12-30 | Fio Corporation | Bio-threat alert system |
CN105759021A (en) | 2008-08-29 | 2016-07-13 | Fio公司 | Single-use Handheld Diagnostic Test Device And Associated System And Method For Testing Biological And Environmental Test Samples |
RU2578023C2 (en) | 2009-01-13 | 2016-03-20 | Эф-Ай-Оу Корпорейшн | Portable diagnostic unit and method for using it with electronic device and diagnostic cartridge in instant diagnostic tests |
US8202486B2 (en) * | 2009-08-12 | 2012-06-19 | Caliper Life Sciences, Inc. | Pinching channels for fractionation of fragmented samples |
US9139426B2 (en) | 2010-09-21 | 2015-09-22 | The University Of North Carolina At Chapel Hill | Methods, systems and devices for forming nanochannels |
US11053535B2 (en) | 2011-09-12 | 2021-07-06 | The University Of North Carolina At Chapel Hill | Devices with a fluid transport nanochannel intersected by a fluid sensing nanochannel and related methods |
EP3591408A1 (en) * | 2012-02-10 | 2020-01-08 | The University of North Carolina at Chapel Hill | Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems |
JP6510984B2 (en) | 2013-02-28 | 2019-05-08 | ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒルThe University Of North Carolina At Chapel Hill | Nanofluidic device with integrated components for controlled capture, capture and transport of macromolecules, and related analysis methods |
CA2903481A1 (en) | 2013-03-13 | 2014-10-09 | The University Of North Carolina At Chapel Hill | Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis |
US10471428B2 (en) | 2015-05-11 | 2019-11-12 | The University Of North Carolina At Chapel Hill | Fluidic devices with nanoscale manifolds for molecular transport, related systems and methods of analysis |
WO2019006190A1 (en) | 2017-06-28 | 2019-01-03 | Bio-Rad Laboratories, Inc. | System and method for droplet detection |
US11045805B2 (en) | 2017-11-01 | 2021-06-29 | Bio-Rad Laboratories, Inc. | Microfluidic system and method for arranging objects |
US10898895B2 (en) | 2018-09-13 | 2021-01-26 | Talis Biomedical Corporation | Vented converging capillary biological sample port and reservoir |
WO2020175381A1 (en) * | 2019-02-27 | 2020-09-03 | 京セラ株式会社 | Particle separation and measurement device, and particle separation and measurement apparatus |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003000416A2 (en) * | 2001-06-20 | 2003-01-03 | Teragenics, Inc. | Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system |
US6506609B1 (en) * | 1999-05-17 | 2003-01-14 | Caliper Technologies Corp. | Focusing of microparticles in microfluidic systems |
US20030159999A1 (en) * | 2002-02-04 | 2003-08-28 | John Oakey | Laminar Flow-Based Separations of Colloidal and Cellular Particles |
EP1380337A2 (en) * | 2002-07-12 | 2004-01-14 | Tosoh Corporation | Fine channel device and a chemically operating method for fluid using the device |
US6770182B1 (en) * | 2000-11-14 | 2004-08-03 | Sandia National Laboratories | Method for producing a thin sample band in a microchannel device |
US20050112606A1 (en) * | 2003-04-10 | 2005-05-26 | Martin Fuchs | Advanced microfluidics |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4793705A (en) * | 1987-10-07 | 1988-12-27 | The United States Of America As Represented By The United States Department Of Energy | Single molecule tracking |
US5274240A (en) * | 1990-01-12 | 1993-12-28 | The Regents Of The University Of California | Capillary array confocal fluorescence scanner and method |
JP3123249B2 (en) * | 1991-09-10 | 2001-01-09 | 株式会社日立製作所 | DNA molecule length measuring method and measuring device |
IL108497A0 (en) * | 1993-02-01 | 1994-05-30 | Seq Ltd | Methods and apparatus for dna sequencing |
DE69825601T2 (en) * | 1997-02-12 | 2005-04-28 | Chan, Eugene Y, Brookline | METHOD FOR THE ANALYSIS OF POLYMERS |
US6790671B1 (en) * | 1998-08-13 | 2004-09-14 | Princeton University | Optically characterizing polymers |
US6818395B1 (en) * | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
EP1463796B1 (en) * | 2001-11-30 | 2013-01-09 | Fluidigm Corporation | Microfluidic device and methods of using same |
-
2006
- 2006-07-13 EP EP06787065A patent/EP1904653A4/en not_active Withdrawn
- 2006-07-13 WO PCT/US2006/027111 patent/WO2007011622A2/en active Application Filing
- 2006-07-13 JP JP2008522826A patent/JP2009501938A/en not_active Withdrawn
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6506609B1 (en) * | 1999-05-17 | 2003-01-14 | Caliper Technologies Corp. | Focusing of microparticles in microfluidic systems |
US6770182B1 (en) * | 2000-11-14 | 2004-08-03 | Sandia National Laboratories | Method for producing a thin sample band in a microchannel device |
WO2003000416A2 (en) * | 2001-06-20 | 2003-01-03 | Teragenics, Inc. | Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system |
US20030159999A1 (en) * | 2002-02-04 | 2003-08-28 | John Oakey | Laminar Flow-Based Separations of Colloidal and Cellular Particles |
EP1380337A2 (en) * | 2002-07-12 | 2004-01-14 | Tosoh Corporation | Fine channel device and a chemically operating method for fluid using the device |
US20050112606A1 (en) * | 2003-04-10 | 2005-05-26 | Martin Fuchs | Advanced microfluidics |
Non-Patent Citations (1)
Title |
---|
See also references of WO2007011622A2 * |
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WO2007011622A3 (en) | 2007-05-24 |
JP2009501938A (en) | 2009-01-22 |
EP1904653A4 (en) | 2010-04-14 |
WO2007011622A2 (en) | 2007-01-25 |
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