EP1013342A3 - Thermal cycling apparatus - Google Patents

Thermal cycling apparatus Download PDF

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Publication number
EP1013342A3
EP1013342A3 EP99125152A EP99125152A EP1013342A3 EP 1013342 A3 EP1013342 A3 EP 1013342A3 EP 99125152 A EP99125152 A EP 99125152A EP 99125152 A EP99125152 A EP 99125152A EP 1013342 A3 EP1013342 A3 EP 1013342A3
Authority
EP
European Patent Office
Prior art keywords
thermal cycler
cover
reaction vessels
lid
base body
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP99125152A
Other languages
German (de)
French (fr)
Other versions
EP1013342B1 (en
EP1013342A2 (en
Inventor
Wolfgang Dr. Heimberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
MWG Biotech AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=7892332&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP1013342(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by MWG Biotech AG filed Critical MWG Biotech AG
Publication of EP1013342A2 publication Critical patent/EP1013342A2/en
Publication of EP1013342A3 publication Critical patent/EP1013342A3/en
Application granted granted Critical
Publication of EP1013342B1 publication Critical patent/EP1013342B1/en
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Abstract

Die Erfindung betrifft eine Thermocyclervorrichtung zur Durchführung chemischer und biologischer Reaktionen und ein Verfahren zum Ansteuern der Thermocyclervorrichtung. Die erfindungsgemäße Thermocyclervorrichtung weist einen Basiskörper auf, an dem zum Aufnehmen eines oder mehrerer nach oben offener Reaktionsgefäße ein Aufnahmebereich ausgebildet ist. Zum Schließen des Aufnahmebereichs des Basiskörpers ist ein Deckel vorgesehen. Federelemente sind derart an der Thermocyclervorrichtung angeordnet, daß der Deckel und das bzw. die Reaktionsgefäße aneinander gedrückt werden können, und die Reaktionsgefäße unmittelbar vom Deckel oder mittels einer Zwischenlage abgeschlossen werden.The invention relates to a thermal cycler device for carrying out chemical and biological reactions and a method for driving the thermal cycler device. The thermal cycler device according to the invention has a base body on which to hold one or more reaction vessels open at the top a recording area is formed. To close the recording area of the A cover is provided for the base body. Spring elements are such on the thermal cycler arranged that the lid and the one or more reaction vessels can be pressed together, and the reaction vessels directly from Cover or be completed with an intermediate layer.

Die Erfindung zeichnet sich durch einen elektrochemischen Linearmotor aus, der derart angeordnet ist, daß der Deckel und das bzw. die Reaktionsgefäße mit einem gegenüber dem durch die Feder erzeugten erhöhten Druck aneinander gedrückt werden können.The invention is characterized by an electrochemical linear motor which is arranged in such a way that the lid and the reaction vessel (s) with a compared to the increased pressure generated by the spring can be.

Das Vorsehen des Linearmotors erlaubt, daß die zusätzliche Druckkraft unabhängig vom Schließvorgang des Deckels ausgeübt wird und ermöglicht, die Druckkraft frei zu variieren und exakt zu dosieren.

Figure 00000001
The provision of the linear motor allows the additional pressure force to be exerted independently of the closing process of the cover and enables the pressure force to be varied freely and to be metered precisely.
Figure 00000001
EP99125152A 1998-12-22 1999-12-16 Thermal cycling apparatus Revoked EP1013342B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19859586 1998-12-22
DE19859586A DE19859586C1 (en) 1998-12-22 1998-12-22 Thermal cycler device

Publications (3)

Publication Number Publication Date
EP1013342A2 EP1013342A2 (en) 2000-06-28
EP1013342A3 true EP1013342A3 (en) 2001-01-10
EP1013342B1 EP1013342B1 (en) 2004-04-07

Family

ID=7892332

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99125152A Revoked EP1013342B1 (en) 1998-12-22 1999-12-16 Thermal cycling apparatus

Country Status (7)

Country Link
US (1) US6153426A (en)
EP (1) EP1013342B1 (en)
JP (1) JP4578604B2 (en)
AT (1) ATE263628T1 (en)
AU (1) AU730946B2 (en)
DE (2) DE19859586C1 (en)
NO (1) NO996187L (en)

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US6640891B1 (en) 2000-09-05 2003-11-04 Kevin R. Oldenburg Rapid thermal cycling device
US7025120B2 (en) * 2000-09-05 2006-04-11 Oldenburg Kevin R Rapid thermal cycling device
US6660232B1 (en) * 2000-09-29 2003-12-09 Promega Corporation Multi-well assay plate and plate holder and method of assembling the same
US7459302B2 (en) * 2001-10-02 2008-12-02 Stratagene California Side-wall heater for thermocycler device
DE20117661U1 (en) * 2001-10-29 2003-03-13 Mwg Biotech Ag Apparatus for heating reaction vessel wells in micro-titration plate has base body to hold them, containing temperature control block which is moved up and down through movements of swing lid
US7614444B2 (en) 2002-01-08 2009-11-10 Oldenburg Kevin R Rapid thermal cycling device
US7373968B2 (en) * 2002-01-08 2008-05-20 Kevin R. Oldenburg Method and apparatus for manipulating an organic liquid sample
US6893613B2 (en) * 2002-01-25 2005-05-17 Bristol-Myers Squibb Company Parallel chemistry reactor with interchangeable vessel carrying inserts
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
US6730883B2 (en) * 2002-10-02 2004-05-04 Stratagene Flexible heating cover assembly for thermal cycling of samples of biological material
US7507376B2 (en) * 2002-12-19 2009-03-24 3M Innovative Properties Company Integrated sample processing devices
US20040191831A1 (en) * 2003-03-25 2004-09-30 Council Of Scientific And Industrial Research Rapid heat - mediated method for enzyme - linked immunosorbent assay procedure
US20050237528A1 (en) * 2003-09-19 2005-10-27 Oldham Mark F Transparent heater for thermocycling
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
US20080118955A1 (en) * 2004-04-28 2008-05-22 International Business Machines Corporation Method for precise temperature cycling in chemical / biochemical processes
US20050244933A1 (en) * 2004-04-28 2005-11-03 International Business Machines Corporation Method and apparatus for precise temperature cycling in chemical/biochemical processes
US20060030037A1 (en) 2004-05-28 2006-02-09 Victor Joseph Thermo-controllable high-density chips for multiplex analyses
US20050282270A1 (en) * 2004-06-21 2005-12-22 Applera Corporation System for thermally cycling biological samples with heated lid and pneumatic actuator
US20060024204A1 (en) * 2004-08-02 2006-02-02 Oldenburg Kevin R Well plate sealing apparatus and method
WO2006012893A1 (en) * 2004-08-05 2006-02-09 Jpk Instruments Ag Device for receiving a test sample
US8252581B2 (en) 2007-01-22 2012-08-28 Wafergen, Inc. Apparatus for high throughput chemical reactions
EP2359933B1 (en) 2007-02-13 2017-11-08 Eppendorf AG Cover for sample with sample-size independent height adjustment
EP1961484B1 (en) * 2007-02-13 2016-07-20 Eppendorf Ag Cover for sample with homogeneous pressure application
US20080318280A1 (en) * 2007-02-13 2008-12-25 Eppendorf Ag Cover for an array of reaction vessels for one-step operation modus
US8865457B2 (en) 2007-03-15 2014-10-21 Siemens Healthcare Diagnostics Inc. Active, micro-well thermal control subsystem
WO2008143959A1 (en) * 2007-05-15 2008-11-27 Wako Pure Chemical Industries, Ltd. Pressure manifold to equalize pressure in integration pcr-ce microfluidic devices
CA2715292C (en) * 2008-02-15 2015-11-24 Bio-Rad Laboratories, Inc. Thermal cycler with self-adjusting lid
US20110156090A1 (en) * 2008-03-25 2011-06-30 Lin Charles W C Semiconductor chip assembly with post/base/post heat spreader and asymmetric posts
DE202008009556U1 (en) 2008-07-16 2009-12-03 Eppendorf Ag Device for tempering at least one sample
CN202830041U (en) * 2009-04-03 2013-03-27 Illumina公司 Device for heating biological sample
US8987685B2 (en) 2009-09-09 2015-03-24 Pcr Max Limited Optical system for multiple reactions
DE102010019232B4 (en) * 2010-05-03 2013-06-27 Eppendorf Ag Avoid condensation hood
GB201018624D0 (en) 2010-11-04 2010-12-22 Epistem Ltd Reaction vessel
DE102011011912B4 (en) * 2011-02-21 2020-09-03 Eppendorf Ag Laboratory device with lid and press arrangement and method for pressing on
CA2908930C (en) 2012-06-28 2021-08-03 Fluoresentric, Inc. A chemical indicator device
CN103725602B (en) * 2012-10-16 2015-05-27 常州福生生物技术有限公司 Thermal cover plate used in nucleic acid amplification detector
EP2976156B1 (en) 2013-03-19 2021-04-07 Life Technologies Corporation Thermal cycler cover
US9829696B2 (en) 2013-05-01 2017-11-28 Bio-Rad Laboratories, Inc. Adjustable digital microscope display
US20140327349A1 (en) * 2013-05-02 2014-11-06 Bio-Rad Laboratories, Inc. Drawer Handle Mechanism
GB2526520B (en) * 2014-04-04 2021-08-18 It Is Int Ltd Biochemical reaction system
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DK3259602T3 (en) 2015-02-20 2021-02-15 Takara Bio Usa Inc PROCEDURE FOR FAST ACCURATE DISPENSATION, VISUALIZATION AND ANALYSIS OF SINGLE CELLS
EP3349903A4 (en) * 2015-09-16 2019-03-13 Fluoresentric, Inc. Apparatus, systems and methods for dynamic flux amplification of samples
EP3387107B1 (en) * 2015-12-11 2020-08-12 Spartan Bioscience Inc. Tube sealing system and methods for nucleic acid amplification
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DE102018124408A1 (en) * 2018-10-02 2020-04-02 Biometra GmbH Device for the thermal treatment of samples
DE102018124412A1 (en) * 2018-10-02 2020-04-02 Biometra GmbH Temperature control block module and device for the thermal treatment of samples
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JP7327360B2 (en) * 2020-11-19 2023-08-16 横河電機株式会社 Heat treatment system, nucleic acid extraction system, nucleic acid analysis system

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US5795547A (en) * 1993-09-10 1998-08-18 Roche Diagnostic Systems, Inc. Thermal cycler
EP0955097A1 (en) * 1998-05-04 1999-11-10 F. Hoffmann-La Roche Ag Thermal cycler having an automatically positionable cover

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US5795547A (en) * 1993-09-10 1998-08-18 Roche Diagnostic Systems, Inc. Thermal cycler
DE4331764C1 (en) * 1993-09-18 1994-11-03 Friwo Silberkraft Ges Fuer Bat Electrochemical actuator
DE4331763C1 (en) * 1993-09-18 1994-11-10 Friwo Silberkraft Ges Fuer Bat Electrochemical actuator
DE19646116A1 (en) * 1996-11-08 1998-05-14 Eppendorf Geraetebau Netheler Temperature control block with recordings
EP0955097A1 (en) * 1998-05-04 1999-11-10 F. Hoffmann-La Roche Ag Thermal cycler having an automatically positionable cover

Also Published As

Publication number Publication date
AU730946B2 (en) 2001-03-22
JP4578604B2 (en) 2010-11-10
EP1013342B1 (en) 2004-04-07
DE59909106D1 (en) 2004-05-13
AU6542799A (en) 2000-06-29
NO996187L (en) 2000-06-23
EP1013342A2 (en) 2000-06-28
ATE263628T1 (en) 2004-04-15
JP2000189152A (en) 2000-07-11
NO996187D0 (en) 1999-12-14
DE19859586C1 (en) 2000-07-13
US6153426A (en) 2000-11-28

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