EP0985453A1 - Zentrifugenkammer für einen Zellseparator - Google Patents
Zentrifugenkammer für einen Zellseparator Download PDFInfo
- Publication number
- EP0985453A1 EP0985453A1 EP99117393A EP99117393A EP0985453A1 EP 0985453 A1 EP0985453 A1 EP 0985453A1 EP 99117393 A EP99117393 A EP 99117393A EP 99117393 A EP99117393 A EP 99117393A EP 0985453 A1 EP0985453 A1 EP 0985453A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- channel
- centrifuge chamber
- fraction
- point
- chamber according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
- B04B2005/045—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation having annular separation channels
Definitions
- the invention relates to a centrifuge chamber for a cell separator, especially for separating blood into several fractions.
- the centrifuge chamber of the known cell separators has one Separation channel into which the cell suspension to be separated is passed. Under the influence of the centrifugal force, a takes place in the separation channel Separation into different fractions, for example platelets (PLT), Erythrocytes (RBC), platelet-rich plasma (PRP) and platelet-poor plasma (PPP), which is withdrawn from the chamber.
- PLT platelets
- RBC Erythrocytes
- PRP platelet-rich plasma
- PPP platelet-poor plasma
- the centrifuge chamber of the known cell separators for separating blood in multiple fractions is generally for single use certainly.
- One and two-part centrifuge chambers are known. Both two-part centrifuge chambers, the separation channel from one flexible film part formed, which is inserted into a rigid receiving unit becomes.
- the separation channel of the known one or two part Centrifuge chambers are designed in one or more stages.
- Centrifuge chambers with a multi-stage separation channel have the disadvantage that that due to turbulence in the transition area between the individual Channel sections already separated cells carried into another fraction can be. For example, there is a risk that already separated Platelets partially or completely mixed with the plasma or Leukocytes are whirled up as a union.
- Single-stage separation chambers have so far been characterized by unclean or inadequate separation, particularly of platelets, since here the Platelets are obtained from the so-called buffy coat, the is naturally heavily contaminated with leukocytes.
- DE-A-28 21 055 describes a multi-stage centrifuge chamber for Separate blood into several fractions, the separation channel of several there are circular sections with different radii are clearly separated from each other by transition areas or dams.
- the Sections of the canal differ significantly in their slope, with the Slope of the channel section at the transition point to which it subsequent section has a break point.
- a centrifuge chamber, the separation channel of several sections is also known from US-A-4,342,420.
- the Separation channel has an entrance area running outwards, one a circular path around the central axis and an end region tapering to the axis of rotation.
- US-A-4 356 958 discloses a single stage separation chamber with one spiral separation channel.
- the separation channel is designed such that it does not run towards the axis of rotation of the chamber, but in Edge area of the chamber runs out.
- the invention has for its object a centrifuge chamber for To create cell separator in which the separation of the cell suspension is very is even and contamination-free.
- the separation channel can consist of one or more channel sections be composed, being between the individual channel sections Areas where liquid is supplied to the separation chamber or Liquid is discharged. In these areas, the interior and The outer wall of the separation channel naturally does not have a continuous course.
- the centrifuge chamber according to the invention is used in particular for separation of whole blood into several fractions, namely erythrocytes and / or Platelets and / or plasma use.
- the separation channel extends up to close to the center of the axis of rotation of the centrifuge chamber.
- the Outlet for the erythrocyte fraction at the radially outer end of the Channel arranged while the outlet for the plasma fraction on the radial inner end of the channel is arranged.
- the entrance for the too separating cell suspension is preferably between the outlet for the Erythrocyte fraction and the outlet for the plasma fraction.
- the platelet fraction outlet is preferably between the inlet for blood and the outlet for the plasma fraction.
- the advantages of Centrifuge chamber, the separation channel of a progressive slope has, especially to wear.
- the progressive slope of the canal is achieved that erythrocytes in the radially outer areas of the Not be packed too compact.
- the hematocrit value of the Erythrocytes can therefore unite in the radially outer areas Do not exceed a maximum value of 80 to 90% hct.
- it is from Advantage as a high hematocrit values in the outer areas of the channel radially inward flow of platelets into the plasma with special needs. It also ensures that plasma is unimpeded across the entire length of the channel can flow radially inward to the plasma outlet.
- the outlet for the Platelets spread over the entire height of the separation channel extending recess on the radially outer side wall of the channel arranged, from which the platelets withdraw with great effectiveness to let.
- the platelets that pass through the Plasma flow from the buffy coat layer on the erythrocytes to the Plasma outlet are entrained as well as the platelets that are caused by the progressive increase of the channel from radially inner areas fall back and get into the recess.
- the outlet for the platelets is advantageously in the lower half of the recess, preferably in the radially outer part of the Deepening.
- the separation channel with the erythrocyte outlet on the radially outside lying and the plasma outlet at the radially inner end can When priming with solutions or blood, vent slightly because the air bubbles under the influence of the centrifugal force to the radially inner end driven where they are removed through the plasma outlet without residue can be.
- the cross section of the separation channel is over the entire length preferably constant. But it is also possible to use a separation channel to provide a cross-section that changes continuously in the longitudinal direction.
- the centrifuge chamber can be designed as a one-piece chamber, wherein the centrifuge channel is part of the (housing body. But it is also possible to form the centrifuge chamber in two parts, the Separation channel as a flexible channel made of hose or film material in the Housing body is used.
- the centrifuge chamber comprises a circular housing body 1, which in the cell separator is inserted.
- the housing body 1 carries a separation channel 3, which is about the axis of rotation 2 Centrifuge chamber extends.
- the separation channel has a first at its outer end 4 Outlet 5 for erythrocytes (RWC) and at its inner end 6 one second outlet 7 for plasma (PLS). Between the erythrocyte outlet 5 and the plasma outlet 7, the separation channel 3 has an inlet 8 for the whole blood to be separated (WB), while between the whole blood inlet 8 and the plasma outlet 7, a third outlet 9 for platelets (PLT) is arranged.
- the inlet and the outlets are in the length of the channel arranged essentially at constant intervals.
- the separation channel 3 has the same cross section along its length. He is by an inner side wall 10 and an outer Side wall 11 and a lower wall 12 and an upper wall 13 limited ( Figure 3).
- the course of the separation channel 3 is shown in the middle between the Side walls 10, 11 extending line described, which is in the form of a Spiral 5 winds around the axis of rotation 2 of the centrifuge chamber and onto the Rotation axis approaches.
- the slope of the spiral 5 describing the course of the rotation channel takes from the outside channel end 4 to the inside channel end 6 steadily, the slope being defined at a point on the spiral than the angle between the tangent of a circle around the axis of rotation in this point and the tangent of the spiral at this point.
- FIG 3 is a point on the course of the separation channel descriptive spiral 5 designated A.
- the circle on which point A and the axis of rotation 2 of the centrifuge chamber is designated by K.
- the slope at point A is now defined as the angle ⁇ between the Tangent T1 of circle K at point A and tangent T2 of the spiral S, which describes the course of the channel, in point A.
- the spiral S describing the course of the channel points from that outer channel end 4 over substantially the first half of its length a slope that is less than 5 ° and greater than 5 ° in the second half is.
- the slope parameter y is less than 1500.
- the whole blood inlet 8 is preferably located on a part of the channel the slope is less than 1 °, while the platelet outlet 9 is preferably on a steep part of the channel on which the slope is greater than Is 5 °.
- Figure 4 shows a section through the separation channel 3 at the level of Platelet outlet 9.
- the outer side wall 11 runs underneath Formation of a recess 15 radially outwards, and then radially again to run inside.
- the bottom of the depression is in the outside Sidewall of the platelet outlet 9 is arranged.
- the recess 15 is formed over the entire channel height so that it the channel cross section does not change significantly in terms of flow and the Laminar flow over the fume cupboard.
- the outside wall of the outside Section of the separation channel goes into an obliquely outward Over the wall, which is followed by a sloping inward wall, which then merges into the radially inner channel section.
- the removal port for the platelets is located at the point of the Separation channel where the two walls meet.
- both the platelets fall through Plasma flow from the buffy coat layer on the erythrocytes to the Plasma outlet 7 are entrained, as well as the platelets by the progressive slope of the channel from radially inner areas fall behind.
- the slope parameter y1 is less than 1500 and the slope parameter y2 is less than 10, where phi1 / phi0 is greater than 0.3.
- the channel can have an angular circumference> 360 °.
- Figure 7 shows the course of the separation channel 3, which is a very over 270 ° asked for a slight slope, which then grows progressively up to 540 °.
- a Separation chamber with such a channel is very suitable platelet-rich plasma. This is at the radially inside deducted lying point.
Abstract
Description
- Figur 1
- eine Zentrifugenkammer in schematischer Darstellung,
- Figur 2
- den Verlauf des Separationskanals der Zentrifugenkammer von Figur 1,
- Figur 3
- einen Schnitt durch den Separationskanal von Figur 1 entlang der Linie III-III von Figur 1 in vergrößerter Darstellung,
- Figur 4
- einen Schnitt durch den Separationskanal von Figur 1 entlang der Linie IV-IV in vergrößerter Darstellung und
- Figuren 5 bis 7
- den Verlauf des Separationskanals weiterer Ausführungsbeispiele der Zentrifugenkammer.
- R =
- radialer Abstand der den Verlauf des Kanals beschreibenden Spirale S an der Steile phi
- R0 =
- radial größter Abstand der den Verlauf des Kanals beschreibenden Spirale S am außenliegenden Kanalanfang
- phi =
- Winkel des betrachteten Kanalpunktes
- phi0 =
- Gesamtwinkel des Kanals
- y =
- Steigungsparameter
- R =
- radialer Abstand der den Verlauf des Separationskanals beschreibenden Spirale an der Stelle phi
- R0 =
- radial größter Kanalabstand am außen liegenden Kanalanfang
- phi =
- Winkel des betrachteten Kanalpunktes
- phi0 =
- Gesamtwinkel des Kanals
- phi1 =
- Winkelparameter
- y1 =
- Steigungsparameter 1
- y2 =
- Steigungsparameter 2
- R0 =
- radial größter Kanalbastand
- phi1 =
- Winkelparameter 1
- y2 =
- Steigungsparmeter 2
- y1 =
- Kreisabweichung bei phi1
- phi0 =
- Gesamtwinkel
- y3 =
- Steilheit
- phi3 =
- Progressivabschnitt
- phi =
- Winkel des betrachteten Kanalpunktes
Claims (11)
- Zentrifugenkammer für einen Zellseparator mit einem Separationskanal (3), der sich aus mindestens einem Kanalabschnitt zusammensetzt, der von einer radial innen liegenden und einer radial außen liegenden Seitenwand (10, 11) begrenzt wird und der einen Einlaß (8) für die zu separierende Zellsuspension, insbesondere Blut, und mindestens einen Auslaß (5) für eine Fraktion der Zellsuspension aufweist, wobei der Verlauf des bzw. jedes Kanalabschnitts durch eine in der Mitte zwischen den Seitenwänden verlaufende Linie beschrieben wird, die sich um die Rotationsachse (2) der Zentrifugenkammer windet,
dadurch gekennzeichnet,
daß die den Verlauf des bzw. jedes Kanalabschnitts des Separationskanals (3) beschreibende Linie eine Spirale (5) ist, die sich von dem radial außen liegenden Kanalende (4) mit zunehmender Steigung zu dem radial innen liegenden Kanalende (6) erstreckt, wobei die Steigung an einem Punkt (A) auf der Spirale definiert ist als der Winkel α zwischen der Tangente (T1) eines Kreises (K) um die Rotationsachse (2) in diesem Punkt und der Tangente (T2) der Spirale in diesem Punkt. - Zentrifugenkammer nach Anspruch 1, dadurch gekennzeichnet, daß die den Verlauf eines radial außen liegenden Kanalabschnitts eines sich aus mehreren Kanalabschnitten zusammensetzenden Separationskanals (3) beschreibende Linie (S) an jedem Punkt der Linie eine Steigung hat, die kleiner als die Steigung an jedem Punkt eines sich an den radial außen liegenden Kanalabschnitt anschließenden Kanalabschnitts ist, der radial innen liegt.
- Zentrifugenkammer nach Anspruch 1 oder 2, dadurch gekennzeichnet, daß sich der Separationskanal bis nahe an die Rotationsachse (2) der Zentrifugenkammer erstreckt.
- Zentrifugenkammer nach einem der Ansprüche 1 bis 3, dadurch gekennzeichnet, daß der Auslaß (5) für eine Fraktion der Zellsuspension, insbesondere die Erythrozytenfraktion, an dem radial außen liegenden Ende (4) des Kanals (3) angeordnet ist.
- Zentrifugenkammer nach einem der Ansprüche 1 bis 4, dadurch gekennzeichnet, daß ein zweiter Auslaß (7) für eine zweite Fraktion der Zellsuspension, insbesondere die Plasmafraktion, an dem radial innen liegenden Ende (6) des Kanals (3) angeordnet ist.
- Zentrifugenkammer nach einem der Ansprüche 1 bis 5, dadurch gekennzeichnet, daß der Einlaß (8) für die zu separierende Zellsuspension, insbesondere Blut, zwischen dem radial innnen liegenden und radial außen liegenden Ende (6, 4) des Kanals (3) angeordnet ist.
- Zentrifugenkammer nach Anspruch 6, dadurch gekennzeichnet, daß ein dritter Auslaß (9) für eine dritte Fraktion der Zellsuspension, insbesondere die Thrombozytenfraktion, zwischen dem Einlaß (8) und dem radial innen liegenden Ende (6) des Kanals (3) angeordnet ist.
- Zentrifugenkammer nach Anspruch 7, dadurch gekennzeichnet, daß der dritte Auslaß (9) für die dritte Zellsuspension, insbesondere die Thrombozytenfraktion, in einer sich im wesentlichen über die gesamte Höhe des Kanals erstreckenden Vertiefung (15) an der radial außen liegenden Seitenwand (11) des Separationskanals (3) angeordnet ist.
- Zentrifugenkammer nach einem der Ansprüche 1 bis 8, dadurch gekennzeichnet, daß der Einlaß (8) und die Auslässe (5, 7, 9) über die Länge des Separationskanals (3) im wesentlichen in gleichbleibenden Abständen verteilt angeordnet sind.
- Zentrifugenkammer nach einem der Ansprüche 1 bis 9, dadurch gekennzeichnet, daß die den Verlauf des Separationskanals (3) beschreibende Spirale (S) ausgehend von dem außen liegenden Ende (4) des Kanals (3) über im wesentlichen die erste Hälfte ihrer Länge eine Steigung aufweist, die kleiner als 5° und in der zweiten Hälfte größer als 5° ist.
- Zentrifugenkammer nach einem der Ansprüche 1 bis 10, dadurch gekennzeichnet, daß der Querschnitt des Separationskanals (3) über die gesamte Länge konstant ist.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19841835 | 1998-09-12 | ||
DE19841835A DE19841835C2 (de) | 1998-09-12 | 1998-09-12 | Zentrifugenkammer für einen Zellseparator |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0985453A1 true EP0985453A1 (de) | 2000-03-15 |
EP0985453B1 EP0985453B1 (de) | 2005-11-23 |
Family
ID=7880779
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP99117393A Expired - Lifetime EP0985453B1 (de) | 1998-09-12 | 1999-09-04 | Zentrifugenkammer für einen Zellseparator |
Country Status (5)
Country | Link |
---|---|
US (1) | US6277060B1 (de) |
EP (1) | EP0985453B1 (de) |
JP (1) | JP4027540B2 (de) |
DE (2) | DE19841835C2 (de) |
ES (1) | ES2248948T3 (de) |
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US4708712A (en) * | 1986-03-28 | 1987-11-24 | Cobe Laboratories, Inc. | Continuous-loop centrifugal separator |
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DE3632500A1 (de) * | 1986-09-24 | 1988-04-07 | Fresenius Ag | Zentrifugenanordnung |
US5904645A (en) * | 1996-05-15 | 1999-05-18 | Cobe Laboratories | Apparatus for reducing turbulence in fluid flow |
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1998
- 1998-09-12 DE DE19841835A patent/DE19841835C2/de not_active Expired - Fee Related
-
1999
- 1999-09-04 DE DE59912818T patent/DE59912818D1/de not_active Expired - Lifetime
- 1999-09-04 ES ES99117393T patent/ES2248948T3/es not_active Expired - Lifetime
- 1999-09-04 EP EP99117393A patent/EP0985453B1/de not_active Expired - Lifetime
- 1999-09-10 JP JP25674799A patent/JP4027540B2/ja not_active Expired - Fee Related
- 1999-09-10 US US09/394,574 patent/US6277060B1/en not_active Expired - Lifetime
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US3698626A (en) * | 1971-05-17 | 1972-10-17 | Atomic Energy Commission | Centrifuge separator |
US4342420A (en) * | 1979-09-28 | 1982-08-03 | Gambro Dialysatoren Kg | Device for separating liquids, especially whole blood |
US4479790A (en) * | 1983-04-22 | 1984-10-30 | Texasgulf, Inc. | Centrifugal separator and method of operating same |
DE4226974A1 (de) * | 1992-08-14 | 1994-02-17 | Fresenius Ag | Verfahren und Vorrichtung zur kontinuierlichen Aufbereitung einer Zellsuspension |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1186346A1 (de) * | 2000-09-05 | 2002-03-13 | Jean-Denis Rochat | Rotorkammer zur Trennung von Blut- oder Plasmakomponenten |
WO2018046268A1 (en) | 2016-09-06 | 2018-03-15 | Fresenius Kabi Deutschland Gmbh | Automated method for leukocyte collection from whole blood |
Also Published As
Publication number | Publication date |
---|---|
DE19841835C2 (de) | 2003-05-28 |
US6277060B1 (en) | 2001-08-21 |
DE59912818D1 (de) | 2005-12-29 |
ES2248948T3 (es) | 2006-03-16 |
DE19841835A1 (de) | 2000-03-23 |
JP2000093506A (ja) | 2000-04-04 |
JP4027540B2 (ja) | 2007-12-26 |
EP0985453B1 (de) | 2005-11-23 |
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