EP0137292B1 - Test tube for immunological analyses - Google Patents
Test tube for immunological analyses Download PDFInfo
- Publication number
- EP0137292B1 EP0137292B1 EP84110414A EP84110414A EP0137292B1 EP 0137292 B1 EP0137292 B1 EP 0137292B1 EP 84110414 A EP84110414 A EP 84110414A EP 84110414 A EP84110414 A EP 84110414A EP 0137292 B1 EP0137292 B1 EP 0137292B1
- Authority
- EP
- European Patent Office
- Prior art keywords
- container
- reaction container
- wall
- reaction
- ribs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
Definitions
- the invention relates to a tubular reaction container for immunological analyzes, which has at least at and next to its closed end an inner wall on which an antibody is fixed, to which the component to be analyzed (antigen) and a labeled component (labeled antigen) of a solution bind in the reaction vessel, the surface of the inner wall of the reaction vessel being enlarged at and near its closed end by the fact that inwardly directed formations are formed in the inner wall which are uniformly distributed over the circumference.
- the formations are designed as flanges which are rectangular in cross section and rounded in side view and which practically only extend over the height of the round bottom of the reaction vessel.
- the the reaction rate i.e. H. the diffusion distance from the liquid space to the wall, which determines the binding rate of the molecules on the wall, is relatively large.
- the inner wall area is too small for some antibodies to be attached. Quite apart from this, it is disadvantageous that gussets are formed between the inner wall of the container and the flanges, which at least make emptying the reaction container more difficult and in which air bubbles can get caught.
- the object of the invention is to improve the function of the generic reaction vessel.
- the solution to this problem is that the formations consist of a maximum of eight round-flank ribs, which extend radially over a length of 1/4 to 1/2 the length of the container by 1/8 to slightly less than 1/2 of the inner diameter of the container extend inward into the container.
- the diffusion distance is short and the inner surface available is more than sufficient in any case. Because the ribs are rounded, i.e. H. between rounded inner wall surfaces, complete emptying of liquid is guaranteed. That is e.g. B. when fixing the antibody on the inner wall of importance, with various chemical treatment and washing steps are required. In the case of immunological experiments, the reaction containers no longer need to be washed if there is complete emptying. This saves time and leads to more uniform test results. Due to the increased inner wall area, the outer diameter of the containers can be reduced, so that conventional analysis devices, which generally place limits on the outer diameter of the reaction containers, can be used. In any case, the reaction containers according to the invention deliver optimal results in practice.
- the container is provided with four to six ribs arranged symmetrically.
- Each rib can protrude from the wall into the reaction container by a piece, which corresponds to 1/5 to 1/3 of the inside diameter of the cross section of the container.
- a particularly advantageous reaction container is a test tube, the lower or closed end of which is provided with five symmetrically arranged ribs on the tube wall, which extend from the wall into the tube by a piece corresponding to approximately 1/3 of the inner diameter of the tube cross section.
- the reaction container according to the invention is preferably made of a plastic or another material containing hydrophilic functional groups, such as -COOH, -OH, -CHO, -NH 2 etc.
- Suitable plastics for the reaction container are, for example, polyacrylate-ethylene copolymers; Polystyrene derivatives, such as polyaminostyrene, polystyrene acrylonitrile or polyvinylbenzyl chloride; Polyhydroxyethyl methacrylate.
- reaction containers can perform immunological analyzes quickly and the possibility of errors decreases because the binding reaction can proceed almost to the end before the solution is removed from the container and the amount of labeled component bound to the wall is measured means a clear benefit to the user when hundreds of analyzes generally need to be performed daily.
- the antibody is fixed to the reaction container in a simple and reliable manner, since the material of the reaction container contains functional groups.
- the surface of the wall of the tubular reaction container is enlarged in that in the wall four ribs S are provided which extend over part of the length of the container.
- the liquid-filled space V in the tube also becomes somewhat narrower.
- the distance between two opposite ribs is about half the pipe diameter.
- the liquid space V is thus divided into four compartments by the ribs S, which protrude into the container by about 1/3 of the diameter corresponding piece.
- the reaction container according to the invention is used as follows: The test sample and the marked constituent are introduced into the reaction container. Thereupon the antigen in the sample and the labeled antigen, which functions as the labeled component, bind to the antibody on the wall of the reaction container. When the binding reaction is sufficiently advanced, the solution is poured from the reaction vessel and the amount of the labeled component adhered to the wall of the reaction vessel is measured. The amount of labeled component can also be measured from the solution poured out of the reaction container. The method of analysis depends on the labeled component used.
- the antibody is fixed to the wall of the reaction container by chemical binding of the antibody to the functional groups of the material from which the reaction container is made.
- reaction vessels are obtained, even when mass-produced, while also ensuring perfect storage.
- the binding of the antigen and the labeled constituent to the antibody fixed to the wall of the reaction container takes place very quickly.
- the reaction container according to the invention it is possible to analyze all such substances and combinations of substances which permit an immunological analysis.
- the antibody fixed to the wall of the reaction container can also be an antigen.
Description
Die Erfindung betrifft einen rohrförmigen Reaktionsbehälter für immunologische Analysen, welcher zumindest an und neben seinem geschlossenen Ende eine Innenwandung auf weist, an welcher ein Antikörper fixiert ist, an welchen sich der zu analysierende Bestandteil (Antigen) und ein markierter Bestandteil (markiertes Antigen) einer Lösung im Reaktionsbehälter binden, wobei die Oberfläche der Innenwandung des Reaktionsbehälters an und nahe seinem geschlossenen Ende dadurch vergrößert ist, daß in der Innenwandung gleichmäßig über den Umfang verteilte, nach innen gerichtete Ausformungen ausgebildet sind.The invention relates to a tubular reaction container for immunological analyzes, which has at least at and next to its closed end an inner wall on which an antibody is fixed, to which the component to be analyzed (antigen) and a labeled component (labeled antigen) of a solution bind in the reaction vessel, the surface of the inner wall of the reaction vessel being enlarged at and near its closed end by the fact that inwardly directed formations are formed in the inner wall which are uniformly distributed over the circumference.
Bei einem bekannten Reaktionsbehälter dieser Art (DE-U1-8331 431) sind die Ausformungen als im Querschnitt rechteckige und in Seitenansicht gerundete Flansche ausgebildet, die sich praktisch nur über die Höhe des Rundbodens des Reaktionsbehälters erstrecken. Die die Reaktionsrate, d. h. die Bindungsrate der Moleküle an der Wandung bestimmende Diffusionsentfemung vom Flüssigkeitsraum zur Wandung ist hierbei verhältnismäßig groß. Trotz der Wandungsausformungen ist die innere Wandungsfläche für manche zu befestigende Antikörper zu klein. Ganz abgesehen davon ist nachteilig, daß zwischen Behälterinnenwandung und Flanschen Zwickel gebildet sind, die das Entleeren des Reaktionsbehälters zumindest erschweren und in denen Luftblasen hängenbleiben können.In a known reaction vessel of this type (DE-U1-8331 431), the formations are designed as flanges which are rectangular in cross section and rounded in side view and which practically only extend over the height of the round bottom of the reaction vessel. The the reaction rate, i.e. H. the diffusion distance from the liquid space to the wall, which determines the binding rate of the molecules on the wall, is relatively large. Despite the wall shapes, the inner wall area is too small for some antibodies to be attached. Quite apart from this, it is disadvantageous that gussets are formed between the inner wall of the container and the flanges, which at least make emptying the reaction container more difficult and in which air bubbles can get caught.
Aufgabe der Erfindung ist es, den gattungsgemäßen Reaktionsbehälter funktionsmäßig zu verbessern.The object of the invention is to improve the function of the generic reaction vessel.
Die Lösung dieser Aufgabe besteht darin, daß die Ausformungen aus höchstens acht rundflankigen Rippen bestehen, die sich über eine Länge von 1/4 bis 1/2 der Länge des Behälters um 1/8 bis etwas weniger als 1/2 des Innendurchmessers des Behälters radial nach innen in den Behälter erstrecken.The solution to this problem is that the formations consist of a maximum of eight round-flank ribs, which extend radially over a length of 1/4 to 1/2 the length of the container by 1/8 to slightly less than 1/2 of the inner diameter of the container extend inward into the container.
Beim erfindungsgemäßen Reaktionsbehälter ist die Diffusionsentfernung kurz und die zur Verfügung stehende Innenfläche in jedem Fall mehr als ausreichend. Weil die Rippen rundflankig sind, d. h. zwischen sich gerundete Innenwandflächen aufweisen, ist vollständiges Entleeren von Flüssigkeit gewährleistet. Das ist z. B. beim Fixieren des Antikörpers an der Innenwandung von Bedeutung, wobei verschiedene chemische Behandlungs- und Waschschritte erforderlich sind. Bei immunologischen Versuchen brauchen die Reaktionsbehälter nicht mehr gewaschen zu werden, wenn vollständiges Entleeren gegeben ist. Das spart Zeit und führt zu gleichmäßigeren Versuchsergebnissen. Aufgrund der vergrößerten Innenwandungsfläche kann der Außendurchmesser der Behälter verkleinert werden, so daß herkömmliche Analysengeräte, die im allgemeinen Grenzen bezüglich des Außendurchmessers der Reaktionsbehälter setzen, eingesetzt werden können. Jedenfalls liefern die erfindungsgemäßen Reaktionsbehälter in der Praxis optimale Ergebnisse.In the reaction container according to the invention, the diffusion distance is short and the inner surface available is more than sufficient in any case. Because the ribs are rounded, i.e. H. between rounded inner wall surfaces, complete emptying of liquid is guaranteed. That is e.g. B. when fixing the antibody on the inner wall of importance, with various chemical treatment and washing steps are required. In the case of immunological experiments, the reaction containers no longer need to be washed if there is complete emptying. This saves time and leads to more uniform test results. Due to the increased inner wall area, the outer diameter of the containers can be reduced, so that conventional analysis devices, which generally place limits on the outer diameter of the reaction containers, can be used. In any case, the reaction containers according to the invention deliver optimal results in practice.
Gemäß einer besonders vorteilhaften Ausbildung ist der Behälter mit vier bis sechs symmetrisch angeordneten Rippen versehen. Jede Rippe kann von der Wandung in den Reaktionsbehälter um ein Stück hineinragen, welches 1/5 bis 1/3 des Innendurchmessers des Behälterquerschnittes entspricht.According to a particularly advantageous embodiment, the container is provided with four to six ribs arranged symmetrically. Each rib can protrude from the wall into the reaction container by a piece, which corresponds to 1/5 to 1/3 of the inside diameter of the cross section of the container.
Ein besonders vorteilhafter Reaktionsbehälter ist ein Testrohr, dessen unteres oder geschlossenes Ende mit fünf symmetrisch angeordneten Rippen an der Rohrwandung versehen ist, welche sich von der Wandung aus in das Rohr um ein etwa 1/3 des Innendurchmessers des Rohrquerschnittes entsprechendes Stück erstrecken.A particularly advantageous reaction container is a test tube, the lower or closed end of which is provided with five symmetrically arranged ribs on the tube wall, which extend from the wall into the tube by a piece corresponding to approximately 1/3 of the inner diameter of the tube cross section.
Der erfindungsgemäße Reaktionsbehälter ist vorzugsweise aus einem Kunststoff oder einem anderen, hydrophile funktionelle Gruppen enthaltenden Material hergestellt, wie beispielsweise -COOH, -OH, -CHO, -NH2 usw. Geeignete Kunststoffe für den Reaktionsbehälter sind beispielsweise Polyacrylat-Äthylen-Copolymere; Polystyrolderivate, wie beispielsweise Polyaminostyrol, Polystyrolacrylnitril oder Polyvinylbenzylchlorid ; Polyhydroxyäthylmethacrylat.The reaction container according to the invention is preferably made of a plastic or another material containing hydrophilic functional groups, such as -COOH, -OH, -CHO, -NH 2 etc. Suitable plastics for the reaction container are, for example, polyacrylate-ethylene copolymers; Polystyrene derivatives, such as polyaminostyrene, polystyrene acrylonitrile or polyvinylbenzyl chloride; Polyhydroxyethyl methacrylate.
Versuche haben gezeigt, daß in dem erfindungsgemäßen Reaktionsbehälter der an der Wandung des Behälters fixierte Antikörper sehr schnell den zu analysierenden Bestandteil (Antigen) und den markierten Bestandteil (markiertes (Antigen) aus der Lösung an sich bindet (Fig. 2). Mit dem erfindungsgemäßen Reaktionsbehälter können die immunologischen Analysen schnell durchgeführt werden und die Möglichkeit von Fehlern nimmt ab, da die Bindungsreaktion fast bis zum Ende vor sich gehen kann, bevor die Lösung aus dem Behälter entfernt wird und die Menge des an der Wandung gebundenen markierten Bestandteils gemessen wird. Dies bedeutet einen eindeutigen Vorteil für den Benutzer, wenn im allgemeinen täglich Hunderte von Analysen durchgeführt werden müssen.Experiments have shown that in the reaction container according to the invention the antibody fixed to the wall of the container very quickly binds the component (antigen) to be analyzed and the labeled component (labeled (antigen)) from the solution (FIG. 2) Reaction containers can perform immunological analyzes quickly and the possibility of errors decreases because the binding reaction can proceed almost to the end before the solution is removed from the container and the amount of labeled component bound to the wall is measured means a clear benefit to the user when hundreds of analyzes generally need to be performed daily.
Der Antikörper wird erfindungsgemäß am Reaktionsbehälter auf einfache und zuverlässige Weise fixiert, da das Material des Reaktionsbehälters funktionelle Gruppen enthält.According to the invention, the antibody is fixed to the reaction container in a simple and reliable manner, since the material of the reaction container contains functional groups.
Die Erfindung wird nachstehend anhand der beiliegenden Zeichnung im einzelnen erläutert; es zeigen
- Fig. 1 einen Längs- und einen Querschnitt durch einen Reaktionsbehälter, welcher durch Trennwände in einzelne Abteile unterteilt ist, und
- Fig. 2 das Bindevermögen des zu analysierenden Bestandteils (Antigen) und des markierten Bestandteils (markiertes Antigen) als eine Funktion der Zeit bei einer Messung mit einem erfindungsgemäßen Reaktionsbehälter (Kurve A) bzw. mit einem herkömmlichen Restrohr (Kurve B).
- Fig. 1 shows a longitudinal and a cross section through a reaction vessel, which is divided into individual compartments by partitions, and
- Fig. 2 shows the binding capacity of the component to be analyzed (antigen) and the labeled component (labeled antigen) as a function of time in a measurement with a reaction container according to the invention (curve A) or with a conventional residual tube (curve B).
Bei der Ausführung gemäß Fig. 1 ist die Oberfläche der Wandung des rohrförmigen Reaktionsbehälters dadurch vergrößert, daß in der Wandung vier Rippen S vorgesehen sind, welche sich über einen Teil der Behälterlänge erstrecken. Auf diese Weise wird auch der flüssigkeitsgefüllte Raum V im Rohr etwas enger. Bei dieser Ausführung beträgt der Abstand zwischen zwei einander gegenüberliegenden Rippen etwa die Hälfte des Rohrdurchmessers. Der Flüssigkeitsraum V ist somit durch die Rippen S in vier Abteile unterteilt, welche in den Behälter um ein etwa 1/3 des Durchmessers entsprechendes Stück hineinragen.In the embodiment according to FIG. 1, the surface of the wall of the tubular reaction container is enlarged in that in the wall four ribs S are provided which extend over part of the length of the container. In this way, the liquid-filled space V in the tube also becomes somewhat narrower. In this embodiment, the distance between two opposite ribs is about half the pipe diameter. The liquid space V is thus divided into four compartments by the ribs S, which protrude into the container by about 1/3 of the diameter corresponding piece.
Der erfindungsgemäße Reaktionsbehälter wird wie folgt verwendet: Die Testprobe und der markierte Bestandteil werden in den Reaktionsbehälter eingebracht. Daraufhin bindet sich das Antigen in der Probe und das markierte Antigen, welches als der markierte Bestandteil fungiert, an den Antikörper an der Wandung des Reaktionsbehälters. Wenn die Bindungsreaktion weit genug fortgeschritten ist, wird dis Lösung aus dem Reaktionsbehälter abgegossen und die Menge des an der Wandung des Reaktionsbehälters anhaftenden markierten Bestandteils gemessen. Die Menge an markiertem Bestandteil kann auch aus der aus dem Reaktionsbehälter ausgegossenen Lösung gemessen werden. Das Analyseverfahren hängt von dem verwendeten markierten Bestandteil ab. Die Fixierung des Antikörpers an der Wandung des Reaktionsbehälters erfolgt durch chemische Bindung des Antikörpers an die funktionellen Gruppen des Materials, aus welchem der Reaktionsbehälter besteht.The reaction container according to the invention is used as follows: The test sample and the marked constituent are introduced into the reaction container. Thereupon the antigen in the sample and the labeled antigen, which functions as the labeled component, bind to the antibody on the wall of the reaction container. When the binding reaction is sufficiently advanced, the solution is poured from the reaction vessel and the amount of the labeled component adhered to the wall of the reaction vessel is measured. The amount of labeled component can also be measured from the solution poured out of the reaction container. The method of analysis depends on the labeled component used. The antibody is fixed to the wall of the reaction container by chemical binding of the antibody to the functional groups of the material from which the reaction container is made.
Auf diese Weise erhält man gleichmäßige Reaktionsbehälter, und zwar selbst bei Herstellung in großen Serien, während außerdem eine einwandfreie Lagerung ermöglicht wird. Aufgrund der Form des erfindungsgemäßen Reaktionsbehälters geht die Bindung des Antigen und des markierten Bestandteils an dem an der Wandung des Reaktionsbehälters fixierten Antikörpers sehr schnell vor sich. Mit dem erfindungsgemäßen Reaktionsbehälter lassen sich alle derartigen Substanzen und Substanzkombinationen analysieren, welche eine immunologische Analyse erlauben. Bei dem an der Wandung des Reaktionsbehälters fixierten « Antikörper kann es sich auch um ein Antigen handeln.In this way, even reaction vessels are obtained, even when mass-produced, while also ensuring perfect storage. Because of the shape of the reaction container according to the invention, the binding of the antigen and the labeled constituent to the antibody fixed to the wall of the reaction container takes place very quickly. With the reaction container according to the invention it is possible to analyze all such substances and combinations of substances which permit an immunological analysis. The antibody fixed to the wall of the reaction container can also be an antigen.
Claims (4)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI833207A FI833207A0 (en) | 1983-09-08 | 1983-09-08 | REAKTIONSKAERL FOER IMMUNOLOGISKA BESTAEMNINGAR |
| FI833207 | 1983-09-08 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| EP0137292A2 EP0137292A2 (en) | 1985-04-17 |
| EP0137292A3 EP0137292A3 (en) | 1986-11-12 |
| EP0137292B1 true EP0137292B1 (en) | 1990-03-28 |
Family
ID=8517705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP84110414A Expired - Lifetime EP0137292B1 (en) | 1983-09-08 | 1984-09-01 | Test tube for immunological analyses |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0137292B1 (en) |
| JP (1) | JPS60155972A (en) |
| DE (1) | DE3481760D1 (en) |
| DK (1) | DK157715C (en) |
| ES (1) | ES292536Y (en) |
| FI (1) | FI833207A0 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8394342B2 (en) | 2008-07-21 | 2013-03-12 | Becton, Dickinson And Company | Density phase separation device |
| US8747781B2 (en) | 2008-07-21 | 2014-06-10 | Becton, Dickinson And Company | Density phase separation device |
| US8794452B2 (en) | 2009-05-15 | 2014-08-05 | Becton, Dickinson And Company | Density phase separation device |
| US9333445B2 (en) | 2008-07-21 | 2016-05-10 | Becton, Dickinson And Company | Density phase separation device |
| US9682373B2 (en) | 1999-12-03 | 2017-06-20 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
| US9694359B2 (en) | 2014-11-13 | 2017-07-04 | Becton, Dickinson And Company | Mechanical separator for a biological fluid |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4665035A (en) * | 1986-05-27 | 1987-05-12 | Josephino Tunac | Fermentation apparatus and systems for the cultivation of microorganisms and other biological entities |
| DE4419971A1 (en) * | 1994-06-08 | 1995-12-14 | Eppendorf Geraetebau Netheler | Polypropylene vessel for heating small quantities of liq. |
| ES2210724T3 (en) | 1997-02-14 | 2004-07-01 | Dendreon Corporation | DEVICE FOR WASHING CELLS AND ASSOCIATED PROCEDURE. |
| AU6366799A (en) * | 1998-12-24 | 2000-07-31 | Sumitomo Bakelite Company Limited | Container for immunologic assay |
| EP1234614A1 (en) * | 2001-02-27 | 2002-08-28 | Pentapharm Gmbh | Metering vessel subdivided by ribs for receiving reagents, its fabrication and use |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE8331431U1 (en) * | 1983-11-02 | 1984-02-23 | Aktieselskabet NUNC, 4000 Roskilde | ROEHRE FOR IMMUNOLOGICAL ADSORPTION ANALYSIS |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1573224A (en) * | 1968-04-26 | 1969-07-04 | ||
| US4111754A (en) * | 1976-11-29 | 1978-09-05 | Hydow Park | Immunological testing devices and methods |
| US4146365A (en) * | 1977-11-07 | 1979-03-27 | Litton Bionetics, Inc. | Affinity detection apparatus |
| FI58839C (en) * | 1979-04-30 | 1981-04-10 | Orion Yhtymae Oy | SAETT ATT UTFOERA ENZYM-IMMUNBESTAEMNING OCH FOER UTFOERANDE AV SAETTET AVSETT REAGENSROER |
| JPS5629168A (en) * | 1979-08-16 | 1981-03-23 | Toyobo Co Ltd | Measuring method of body fluid component by immunity chemical reaction |
-
1983
- 1983-09-08 FI FI833207A patent/FI833207A0/en not_active Application Discontinuation
-
1984
- 1984-09-01 EP EP84110414A patent/EP0137292B1/en not_active Expired - Lifetime
- 1984-09-01 DE DE8484110414T patent/DE3481760D1/en not_active Expired - Fee Related
- 1984-09-07 JP JP18663384A patent/JPS60155972A/en active Pending
- 1984-09-07 DK DK429284A patent/DK157715C/en not_active IP Right Cessation
- 1984-09-07 ES ES1984292536U patent/ES292536Y/en not_active Expired
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE8331431U1 (en) * | 1983-11-02 | 1984-02-23 | Aktieselskabet NUNC, 4000 Roskilde | ROEHRE FOR IMMUNOLOGICAL ADSORPTION ANALYSIS |
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9682373B2 (en) | 1999-12-03 | 2017-06-20 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
| US8747781B2 (en) | 2008-07-21 | 2014-06-10 | Becton, Dickinson And Company | Density phase separation device |
| US9933344B2 (en) | 2008-07-21 | 2018-04-03 | Becton, Dickinson And Company | Density phase separation device |
| US8394342B2 (en) | 2008-07-21 | 2013-03-12 | Becton, Dickinson And Company | Density phase separation device |
| US9714890B2 (en) | 2008-07-21 | 2017-07-25 | Becton, Dickinson And Company | Density phase separation device |
| US9333445B2 (en) | 2008-07-21 | 2016-05-10 | Becton, Dickinson And Company | Density phase separation device |
| US9339741B2 (en) | 2008-07-21 | 2016-05-17 | Becton, Dickinson And Company | Density phase separation device |
| US9700886B2 (en) | 2008-07-21 | 2017-07-11 | Becton, Dickinson And Company | Density phase separation device |
| US9452427B2 (en) | 2008-07-21 | 2016-09-27 | Becton, Dickinson And Company | Density phase separation device |
| US9919309B2 (en) | 2009-05-15 | 2018-03-20 | Becton, Dickinson And Company | Density phase separation device |
| US9364828B2 (en) | 2009-05-15 | 2016-06-14 | Becton, Dickinson And Company | Density phase separation device |
| US9079123B2 (en) | 2009-05-15 | 2015-07-14 | Becton, Dickinson And Company | Density phase separation device |
| US9731290B2 (en) | 2009-05-15 | 2017-08-15 | Becton, Dickinson And Company | Density phase separation device |
| US9802189B2 (en) | 2009-05-15 | 2017-10-31 | Becton, Dickinson And Company | Density phase separation device |
| US8998000B2 (en) | 2009-05-15 | 2015-04-07 | Becton, Dickinson And Company | Density phase separation device |
| US9919308B2 (en) | 2009-05-15 | 2018-03-20 | Becton, Dickinson And Company | Density phase separation device |
| US9919307B2 (en) | 2009-05-15 | 2018-03-20 | Becton, Dickinson And Company | Density phase separation device |
| US8794452B2 (en) | 2009-05-15 | 2014-08-05 | Becton, Dickinson And Company | Density phase separation device |
| US10807088B2 (en) | 2009-05-15 | 2020-10-20 | Becton, Dickinson And Company | Density phase separation device |
| US11351535B2 (en) | 2009-05-15 | 2022-06-07 | Becton, Dickinson And Company | Density phase separation device |
| US11786895B2 (en) | 2009-05-15 | 2023-10-17 | Becton, Dickinson And Company | Density phase separation device |
| US9694359B2 (en) | 2014-11-13 | 2017-07-04 | Becton, Dickinson And Company | Mechanical separator for a biological fluid |
Also Published As
| Publication number | Publication date |
|---|---|
| ES292536Y (en) | 1987-08-16 |
| DK157715C (en) | 1990-07-09 |
| JPS60155972A (en) | 1985-08-16 |
| DE3481760D1 (en) | 1990-05-03 |
| EP0137292A2 (en) | 1985-04-17 |
| DK429284A (en) | 1985-03-09 |
| FI833207A0 (en) | 1983-09-08 |
| DK157715B (en) | 1990-02-05 |
| DK429284D0 (en) | 1984-09-07 |
| EP0137292A3 (en) | 1986-11-12 |
| ES292536U (en) | 1987-01-01 |
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