DE69837913D1 - Verfahren zur vervielfältigung von nukleinsäure - Google Patents

Verfahren zur vervielfältigung von nukleinsäure

Info

Publication number
DE69837913D1
DE69837913D1 DE69837913T DE69837913T DE69837913D1 DE 69837913 D1 DE69837913 D1 DE 69837913D1 DE 69837913 T DE69837913 T DE 69837913T DE 69837913 T DE69837913 T DE 69837913T DE 69837913 D1 DE69837913 D1 DE 69837913D1
Authority
DE
Germany
Prior art keywords
nucleic acid
acid molecules
making
nucleotide
primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69837913T
Other languages
English (en)
Other versions
DE69837913T2 (de
Inventor
Eric Kawashima
Laurent Farinelli
Pascal Mayer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Solexa Ltd Great Britain
Solexa Inc
Original Assignee
Solexa Ltd Great Britain
Solexa Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27451621&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69837913(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB9706528.8A external-priority patent/GB9706528D0/en
Priority claimed from GBGB9706529.6A external-priority patent/GB9706529D0/en
Priority claimed from GBGB9713236.9A external-priority patent/GB9713236D0/en
Priority claimed from GBGB9713238.5A external-priority patent/GB9713238D0/en
Application filed by Solexa Ltd Great Britain, Solexa Inc filed Critical Solexa Ltd Great Britain
Application granted granted Critical
Publication of DE69837913D1 publication Critical patent/DE69837913D1/de
Publication of DE69837913T2 publication Critical patent/DE69837913T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
DE69837913T 1997-04-01 1998-04-01 Verfahren zur vervielfältigung von nukleinsäure Expired - Lifetime DE69837913T2 (de)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
GB9706529 1997-04-01
GBGB9706528.8A GB9706528D0 (en) 1997-04-01 1997-04-01 Invention
GB9706528 1997-04-01
GBGB9706529.6A GB9706529D0 (en) 1997-04-01 1997-04-01 Invention
GBGB9713236.9A GB9713236D0 (en) 1997-06-23 1997-06-23 Invention
GB9713236 1997-06-23
GB9713238 1997-06-23
GBGB9713238.5A GB9713238D0 (en) 1997-06-23 1997-06-23 Invention
PCT/GB1998/000961 WO1998044151A1 (en) 1997-04-01 1998-04-01 Method of nucleic acid amplification

Publications (2)

Publication Number Publication Date
DE69837913D1 true DE69837913D1 (de) 2007-07-26
DE69837913T2 DE69837913T2 (de) 2008-02-07

Family

ID=27451621

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69837913T Expired - Lifetime DE69837913T2 (de) 1997-04-01 1998-04-01 Verfahren zur vervielfältigung von nukleinsäure

Country Status (9)

Country Link
US (8) US7985565B2 (de)
EP (4) EP3034626A1 (de)
JP (1) JP2002503954A (de)
AT (2) ATE364718T1 (de)
AU (1) AU6846698A (de)
DE (1) DE69837913T2 (de)
ES (1) ES2563643T3 (de)
HK (1) HK1155784A1 (de)
WO (1) WO1998044151A1 (de)

Families Citing this family (633)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3034626A1 (de) 1997-04-01 2016-06-22 Illumina Cambridge Limited Verfahren zur vervielfältigung von nukleinsäuren
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
DE59905227D1 (de) * 1998-10-21 2003-05-28 Kiedrowski Guenter Von Verfahren zur exponentiellen amplifikation molekularer matrizen
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US20020119448A1 (en) * 1999-06-23 2002-08-29 Joseph A. Sorge Methods of enriching for and identifying polymorphisms
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
WO2001032935A2 (en) * 1999-11-02 2001-05-10 Celine Hu Molecular microarrays and methods for production and use thereof
EP1255860A2 (de) * 1999-12-29 2002-11-13 Mergen Ltd. Festphasen multiplex amplifizierung von polynukleotiden
DE10010282B4 (de) 2000-02-25 2006-11-16 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010280B4 (de) * 2000-02-25 2006-08-10 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
WO2001062982A2 (en) * 2000-02-25 2001-08-30 Mosaic Technologies, Inc. Methods for multi-stage solid phase amplification of nucleic acids
AU2001243640A1 (en) * 2000-03-14 2001-09-24 Investigen Compositions and methods for simultaneous detection of multiple biological entities
DE10027040A1 (de) * 2000-06-02 2001-12-06 Basf Lynx Bioscience Ag Verfahren zur Herstellung von DNA-Arrays
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP3034627B1 (de) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massives paralleles verfahren zum entschlüsseln von dna und rna
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US6858413B2 (en) 2000-12-13 2005-02-22 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
DE60222628T2 (de) * 2001-01-30 2008-07-17 Solexa Ltd., Saffron Walden Herstellung von matrizen aus polynukleotiden
DE10106320A1 (de) * 2001-02-09 2002-08-22 Axaron Bioscience Ag Erzeugung und Verwendung von Zufallsanordnungen klonaler Nukleinsäureinseln auf einer Oberfläche
MXPA02012739A (es) 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
ATE439454T1 (de) 2001-04-02 2009-08-15 Point 2 Point Genomics Ltd Analyse von polynukleotiden mittels kombinatorischer pcr
AU2002307371B2 (en) 2001-04-20 2008-04-03 Pennsylvania State University Methods for nucleic acid manipulation
EP1256632A3 (de) * 2001-05-07 2004-01-02 Smithkline Beecham Corporation Hochdurchsatz-Screening von Polymorphismen
JP4395133B2 (ja) * 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク． Ｄｎａの単一分子増幅および検出
GB0302058D0 (en) * 2003-01-29 2003-02-26 Univ Cranfield Replication of nucleic acid arrays
ES2342665T3 (es) * 2003-01-29 2010-07-12 454 Corporation Secuenciacion desde dos extremos.
CA2521084A1 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
EP2202322A1 (de) * 2003-10-31 2010-06-30 AB Advanced Genetic Analysis Corporation Verfahren zur Herstellung eines gepaarten Markers aus einer Nukleinsäuresequenz und Verwendungsverfahren dafür
EP3175914A1 (de) 2004-01-07 2017-06-07 Illumina Cambridge Limited Verbesserungen in oder im zusammenhang mit molekül-arrays
GB0400584D0 (en) * 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
GB0402895D0 (en) * 2004-02-10 2004-03-17 Solexa Ltd Arrayed polynucleotides
JP4541731B2 (ja) * 2004-03-12 2010-09-08 キヤノン株式会社 核酸検出方法
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
WO2006110855A2 (en) * 2005-04-12 2006-10-19 454 Life Sciences Corporation Methods for determining sequence variants using ultra-deep sequencing
CA2611671C (en) 2005-06-15 2013-10-08 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB0514909D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Methods of nucleic acid amplification and sequencing
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
US7932029B1 (en) * 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
EP1987159B2 (de) 2006-02-08 2020-08-12 Illumina Cambridge Limited Verfahren zur sequenzierung einer polynukleotidmatrize
EP2021503A1 (de) * 2006-03-17 2009-02-11 Solexa Ltd. Isothermische methoden zur entstehung von arrays von einzelnen molekülen
WO2007109749A2 (en) * 2006-03-21 2007-09-27 Wyeth Methods for preventing and treating amyloidogenic diseases
US20070231823A1 (en) * 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
CN101460953B (zh) 2006-03-31 2012-05-30 索雷克萨公司 用于合成分析的序列的系统和装置
EP2032686B1 (de) 2006-06-23 2022-01-12 Illumina, Inc. Vorrichtung und verfahren zur erzeugung von dna-cluster-anordnungen
US9328378B2 (en) * 2006-07-31 2016-05-03 Illumina Cambridge Limited Method of library preparation avoiding the formation of adaptor dimers
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
EP2076609A1 (de) 2006-10-10 2009-07-08 Illumina Inc. Zusammensetzungen und verfahren zur repräsentativen auswahl von nukleinsäuren aus komplexen gemischen mittels hybridisierung
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
JP2010521156A (ja) 2007-03-16 2010-06-24 ４５４ ライフ サイエンシーズ コーポレイション Ｈｉｖ薬物耐性バリアントの検出のためのシステムおよび方法
JP5020734B2 (ja) * 2007-07-31 2012-09-05 株式会社日立ハイテクノロジーズ 核酸解析方法及び装置
EP2201021A4 (de) * 2007-08-29 2012-01-25 Applied Biosystems Llc Alternative nukleinsäuresequenzierverfahren
WO2009032167A1 (en) * 2007-08-29 2009-03-12 Illumina Cambridge Method for sequencing a polynucleotide template
ITBO20070627A1 (it) * 2007-09-14 2009-03-15 Twof Inc Metodo per la preparazione di dna microarray con sonde ad alta densita' lineare
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US20110014611A1 (en) 2007-10-19 2011-01-20 Jingyue Ju Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequences by synthesis
US9540637B2 (en) 2008-01-09 2017-01-10 Life Technologies Corporation Nucleic acid adaptors and uses thereof
US8530197B2 (en) 2008-01-09 2013-09-10 Applied Biosystems, Llc Method of making a paired tag library for nucleic acid sequencing
US8202691B2 (en) * 2008-01-25 2012-06-19 Illumina, Inc. Uniform fragmentation of DNA using binding proteins
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US20090226975A1 (en) * 2008-03-10 2009-09-10 Illumina, Inc. Constant cluster seeding
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
US20110105356A1 (en) * 2008-05-07 2011-05-05 Derosier Chad F Compositions and methods for providing substances to and from an array
EP2318552B1 (de) 2008-09-05 2016-11-23 TOMA Biosciences, Inc. Verfahren zum stratifizieren und annotieren der behandlungsoptionen eines krebsmedikaments
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
FR2936246A1 (fr) * 2008-09-24 2010-03-26 Haploys Methode d'amplification d'acides nucleiques et ses applications.
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
US20100087325A1 (en) * 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
CA2742298C (en) 2008-11-03 2019-09-10 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
JP5798926B2 (ja) 2008-11-07 2015-10-21 シーケンタ インコーポレイテッド 配列解析によって病態をモニターする方法
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
GB0821981D0 (en) * 2008-12-02 2009-01-07 Genome Res Ltd Method for use in polynucleotide sequencing
US8993230B2 (en) * 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
US8236532B2 (en) 2008-12-23 2012-08-07 Illumina, Inc. Multibase delivery for long reads in sequencing by synthesis protocols
EP2387627B1 (de) 2009-01-15 2016-03-30 Adaptive Biotechnologies Corporation Adaptive immunitätsprofilierung und verfahren zur erzeugung monoklonaler antikörper
WO2010117817A2 (en) 2009-03-30 2010-10-14 Life Technologies Corporation Methods for generating target specific probes for solution based capture
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
AU2010242073C1 (en) 2009-04-30 2015-12-24 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
EP2427572B1 (de) * 2009-05-01 2013-08-28 Illumina, Inc. Sequenzierungsverfahren
EP2248914A1 (de) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Die Verwendung von IIB-Restriktionsendonukleasen in Sequenzierungsanwendungen der zweiten Generation
US9309557B2 (en) * 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
CA2765949C (en) 2009-06-25 2016-03-29 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
US10036063B2 (en) 2009-07-24 2018-07-31 Illumina, Inc. Method for sequencing a polynucleotide template
CN102597256B (zh) 2009-08-25 2014-12-03 伊鲁米那股份有限公司 选择和扩增多核苷酸的方法
EP2940153B1 (de) 2009-09-02 2020-05-13 Bio-Rad Laboratories, Inc. System zum Mischen von Flüssigkeiten durch Koaleszenz mehrerer Emulsionen
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
US20110104693A1 (en) * 2009-11-03 2011-05-05 High Throughput Genomics, Inc. QUANTITATIVE NUCLEASE PROTECTION SEQUENCING (qNPS)
SG10201407883PA (en) 2009-12-07 2015-01-29 Illumina Inc Multi-sample indexing for multiplex genotyping
ES2704701T3 (es) 2010-01-19 2019-03-19 Verinata Health Inc Nuevo protocolo de preparación de bibliotecas de secuenciación
US9260745B2 (en) 2010-01-19 2016-02-16 Verinata Health, Inc. Detecting and classifying copy number variation
US9323888B2 (en) 2010-01-19 2016-04-26 Verinata Health, Inc. Detecting and classifying copy number variation
WO2011091063A1 (en) 2010-01-19 2011-07-28 Verinata Health, Inc. Partition defined detection methods
WO2011091046A1 (en) 2010-01-19 2011-07-28 Verinata Health, Inc. Identification of polymorphic sequences in mixtures of genomic dna by whole genome sequencing
US10388403B2 (en) 2010-01-19 2019-08-20 Verinata Health, Inc. Analyzing copy number variation in the detection of cancer
US20120010085A1 (en) 2010-01-19 2012-01-12 Rava Richard P Methods for determining fraction of fetal nucleic acids in maternal samples
US20120100548A1 (en) 2010-10-26 2012-04-26 Verinata Health, Inc. Method for determining copy number variations
US20110312503A1 (en) 2010-01-23 2011-12-22 Artemis Health, Inc. Methods of fetal abnormality detection
EP2539464B1 (de) 2010-02-23 2016-11-16 Illumina, Inc. Verfahren zur minimierung sequenzabhängiger amplifikationsunterschiede
US8399198B2 (en) 2010-03-02 2013-03-19 Bio-Rad Laboratories, Inc. Assays with droplets transformed into capsules
EP2550528B1 (de) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Tropfenerzeugung für tropfenbasierte prüfungen
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
WO2011127099A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
CA2798431C (en) * 2010-05-06 2018-10-23 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
CA2798758C (en) 2010-05-18 2019-05-07 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
US8483969B2 (en) 2010-09-17 2013-07-09 Illuminia, Inc. Variation analysis for multiple templates on a solid support
KR20130113447A (ko) * 2010-09-24 2013-10-15 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 고정된 프라이머들을 이용하여 표적 dna의 직접적인 캡쳐, 증폭 및 서열화
US8759038B2 (en) 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
WO2012048341A1 (en) 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
EP2633069B1 (de) 2010-10-26 2015-07-01 Illumina, Inc. Sequenzierungsverfahren
EP2632593B1 (de) 2010-10-27 2021-09-29 Illumina, Inc. Durchflusszellen zur biologischen oder chemischen analyse
CA3140602C (en) 2010-11-01 2023-10-03 Bio-Rad Laboratories, Inc. System for forming emulsions
US8575071B2 (en) 2010-11-03 2013-11-05 Illumina, Inc. Reducing adapter dimer formation
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2635679B1 (de) 2010-11-05 2017-04-19 Illumina, Inc. Verknüpfung von sequenzstücken mit gepaarten code-tags
EP2643484A4 (de) 2010-11-22 2014-04-16 Univ California Verfahren zur identifizierung eines rna-transkripts zellulären ursprungs
CN103370425B (zh) 2010-12-17 2019-03-19 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
US20130344540A1 (en) * 2011-02-03 2013-12-26 Mark T. Reed Methods for minimizing sequence specific bias
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
ES2907069T3 (es) 2011-04-12 2022-04-21 Verinata Health Inc Resolución de fracciones genómicas usando recuentos de polimorfismos
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
GB2484764B (en) 2011-04-14 2012-09-05 Verinata Health Inc Normalizing chromosomes for the determination and verification of common and rare chromosomal aneuploidies
US9411937B2 (en) 2011-04-15 2016-08-09 Verinata Health, Inc. Detecting and classifying copy number variation
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
EP2702175B1 (de) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzungen zur nukleinsäureanalyse
CA2834976C (en) 2011-05-04 2016-03-15 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection assay (qnpa) and sequencing (qnps) improvements
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US10559048B2 (en) 2011-07-13 2020-02-11 The Multiple Myeloma Research Foundation, Inc. Methods for data collection and distribution
WO2013012440A2 (en) * 2011-07-21 2013-01-24 Cornell University Methods and devices for dna sequencing and molecular diagnostics
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
SI3623481T1 (sl) 2011-09-23 2022-01-31 Illumina, Inc. Sestavki za sekvenciranje nukleinske kisline
WO2013049135A1 (en) 2011-09-26 2013-04-04 Gen-Probe Incorporated Algorithms for sequence determinations
CA2852665A1 (en) 2011-10-17 2013-04-25 Good Start Genetics, Inc. Analysis methods
WO2013059746A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
GB2495909A (en) * 2011-10-19 2013-05-01 Genome Analysis Ct Arrays comprising immobilized primer pair spots for amplifying target nucleic acids
CA2853088C (en) 2011-10-21 2018-03-13 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
CA2854023A1 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
CN104105797B (zh) 2011-12-01 2016-08-31 吉纳普赛斯股份有限公司 用于高效电子测序与检测的系统和方法
DK2788499T3 (en) 2011-12-09 2016-03-21 Illumina Inc Enhanced root for polymer tags
EP3904536A1 (de) 2011-12-09 2021-11-03 Adaptive Biotechnologies Corporation Diagnose von lymphoid-malignität und nachweis minimaler resterkrankungen
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
US9823246B2 (en) 2011-12-28 2017-11-21 The Board Of Trustees Of The Leland Stanford Junior University Fluorescence enhancing plasmonic nanoscopic gold films and assays based thereon
CN104334739A (zh) 2012-01-13 2015-02-04 Data生物有限公司 通过新一代测序进行基因分型
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
WO2013117595A2 (en) 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
US10011871B2 (en) 2012-02-17 2018-07-03 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
EP3372694A1 (de) 2012-03-05 2018-09-12 Adaptive Biotechnologies Corporation Festlegung von gepaarten immunrezeptorketten aus frequenzabgestimmten untereinheiten
NO2694769T3 (de) 2012-03-06 2018-03-03
ES2828661T3 (es) 2012-03-20 2021-05-27 Univ Washington Through Its Center For Commercialization Métodos para reducir la tasa de error de la secuenciación de ADN masiva en paralelo mediante el uso de la secuenciación de secuencia consenso bicatenaria
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US9444880B2 (en) 2012-04-11 2016-09-13 Illumina, Inc. Cloud computing environment for biological data
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
CN107586832B (zh) 2012-05-08 2021-03-30 适应生物技术公司 用于测量和校准多重pcr反应中的扩增偏倚的组合物和方法
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
GB2518078B (en) 2012-06-18 2015-04-29 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
EP2870264A4 (de) 2012-07-03 2016-03-02 Sloan Kettering Inst Cancer Quantitative beurteilung der wiederherstellung eines menschlichen t-zellen-repertoires nach der transplantation allogener blutbildender stammzellen
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
ES2637538T3 (es) 2012-07-17 2017-10-13 Counsyl, Inc. Sistema y métodos para la detección de una variación genética
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
ES2660027T3 (es) 2012-10-01 2018-03-20 Adaptive Biotechnologies Corporation Evaluación de la inmunocompetencia por la diversidad de los receptores de inmunidad adaptativa y caracterización de la clonalidad
US9365896B2 (en) 2012-10-19 2016-06-14 Agilent Technologies, Inc. Addition of an adaptor by invasive cleavage
ES2886507T3 (es) 2012-10-29 2021-12-20 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
US9116139B2 (en) 2012-11-05 2015-08-25 Illumina, Inc. Sequence scheduling and sample distribution techniques
EP2746405B1 (de) 2012-12-23 2015-11-04 HS Diagnomics GmbH Verfahren und Primersätze zur PCR-Hochdurchsatzsequenzierung
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014108850A2 (en) 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
US9805407B2 (en) 2013-01-25 2017-10-31 Illumina, Inc. Methods and systems for using a cloud computing environment to configure and sell a biological sample preparation cartridge and share related data
DK2964624T3 (en) 2013-03-08 2017-04-03 Illumina Cambridge Ltd RHODAMINE COMPOUNDS AND USE AS FLUORESCING LABELS
AU2013380645B2 (en) 2013-03-08 2018-03-15 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
EP3919617A1 (de) 2013-03-13 2021-12-08 Illumina, Inc. Verfahren und zusammensetzungen zur nukleinsäuresequenzierung
US8778609B1 (en) 2013-03-14 2014-07-15 Good Start Genetics, Inc. Methods for analyzing nucleic acids
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
EP2971130A4 (de) 2013-03-15 2016-10-05 Nugen Technologies Inc Sequentielle sequenzierung
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
EP2986762B1 (de) 2013-04-19 2019-11-06 Bio-Rad Laboratories, Inc. Digitale analyse von analyten
US8847799B1 (en) 2013-06-03 2014-09-30 Good Start Genetics, Inc. Methods and systems for storing sequence read data
WO2014210225A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
MX2016000997A (es) 2013-07-25 2016-04-19 Dch Molecular Diagnostics Inc Metodos y composiciones para detectar contaminacion bacteriana.
US9352315B2 (en) 2013-09-27 2016-05-31 Taiwan Semiconductor Manufacturing Company, Ltd. Method to produce chemical pattern in micro-fluidic structure
EP3058096A1 (de) 2013-10-18 2016-08-24 Good Start Genetics, Inc. Verfahren zur beurteilung eines genomischen bereichs einer person
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US10415083B2 (en) 2013-10-28 2019-09-17 The Translational Genomics Research Institute Long insert-based whole genome sequencing
US10540783B2 (en) * 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
EP3715467A1 (de) 2013-12-03 2020-09-30 Illumina, Inc. Verfahren und systeme zur analyse von bilddaten
CN106460032B (zh) 2013-12-05 2019-12-24 生捷科技控股公司 图案化阵列的制备
CN105940024B (zh) * 2013-12-05 2019-03-15 生捷科技控股公司 修饰的表面
US10597715B2 (en) 2013-12-05 2020-03-24 Centrillion Technology Holdings Methods for sequencing nucleic acids
EP3080585B1 (de) 2013-12-10 2023-12-06 Illumina, Inc. Biosensoren zur biologischen oder chemischen analyse und verfahren zur herstellung davon
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3792921A1 (de) 2013-12-11 2021-03-17 Genapsys, Inc. Systeme und verfahren für biologische analysen und berechnungen
EP3080298B1 (de) 2013-12-11 2018-10-31 AccuraGen Holdings Limited Verfahren für den nachweis seltener sequenzvarianten
EP3083994B1 (de) 2013-12-20 2021-08-18 Illumina, Inc. Konservierung von genom-verbindungsinformationen in fragmentierten genomischen dna-proben
WO2015103225A1 (en) 2013-12-31 2015-07-09 Illumina, Inc. Addressable flow cell using patterned electrodes
US9926598B2 (en) 2014-01-16 2018-03-27 Illumina, Inc. Amplicon preparation and sequencing on solid supports
US9944924B2 (en) 2014-01-16 2018-04-17 Illumina, Inc. Polynucleotide modification on solid support
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
US10438244B2 (en) 2014-02-13 2019-10-08 Illumina, Inc. Integrated consumer genomic services
NZ723399A (en) 2014-02-18 2020-08-28 Illumina Inc Methods and compositions for dna profiling
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US20170292149A1 (en) 2014-03-05 2017-10-12 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
PL3116900T3 (pl) 2014-03-09 2021-03-08 The Trustees Of The University Of Pennsylvania Kompozycje użyteczne w leczeniu niedoboru transkarbamylazy ornitynowej (otc)
CN106459869B (zh) 2014-03-11 2020-01-21 伊鲁米那股份有限公司 一次性的集成微流体盒及其制备和使用的方法
EP2921556A1 (de) 2014-03-21 2015-09-23 Lexogen GmbH Anzahlerhaltendes RNA-Analyseverfahren
US11060139B2 (en) 2014-03-28 2021-07-13 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
ES2777529T3 (es) 2014-04-17 2020-08-05 Adaptive Biotechnologies Corp Cuantificación de genomas de células inmunitarias adaptativas en una mezcla compleja de células
EP3132060B1 (de) 2014-04-18 2019-03-13 Genapsys Inc. Verfahren und systeme zur nukleinsäureamplifikation
AU2015249846B2 (en) 2014-04-21 2021-07-22 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
WO2015166768A1 (ja) * 2014-05-02 2015-11-05 国立大学法人金沢大学 単一細胞由来核酸の解析方法
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
EP3140428B1 (de) * 2014-05-08 2021-07-28 Fluidigm Corporation Verfahren zur markierung von nukleinsäuresequenzen
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
SG11201610168YA (en) 2014-05-16 2017-01-27 Illumina Inc Nucleic acid synthesis techniques
CA3127071A1 (en) 2014-05-27 2015-12-03 Alex Aravanis Systems and methods for biochemical analysis including a base instrument and a removable cartridge
GB201409777D0 (en) 2014-06-02 2014-07-16 Illumina Cambridge Ltd Methods of reducing density-dependent GC bias in isothermal amplification
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
GB201410420D0 (en) 2014-06-11 2014-07-23 Illumina Cambridge Ltd Methods for estimating cluster numbers
GB201410646D0 (en) * 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
CA2957633A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
CA2956723C (en) 2014-08-12 2023-04-11 Nextgen Jane, Inc. System and method for monitoring health based on collected bodily fluid
US10174383B2 (en) 2014-08-13 2019-01-08 Vanadis Diagnostics Method of estimating the amount of a methylated locus in a sample
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
ES2727656T3 (es) 2014-09-15 2019-10-17 Abvitro Llc Secuenciación de alto rendimiento de banco de nucleótidos
WO2016048829A1 (en) 2014-09-24 2016-03-31 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
US9574991B2 (en) 2014-10-14 2017-02-21 Omnivision Technologies, Inc. High-throughput fluorescence imaging system and device with sample heating capability, and associated methods
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
AU2015331739B2 (en) 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition
EP3212790B1 (de) 2014-10-29 2020-03-25 Adaptive Biotechnologies Corp. Hochmultiplexierter gleichzeitiger nachweis von für adaptive gepaarte immunrezeptorheterodimere codierenden nukleinsäuren aus einer vielzahl von proben
DK3215616T3 (da) 2014-11-05 2020-03-02 Illumina Cambridge Ltd Reducering af DNA-skade under prøvepræparering og sekventering ved anvendelse af siderophor-chelatorer
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
CN111024936A (zh) 2014-11-05 2020-04-17 纳迈达斯生物科技中心 用于增强成像的金属复合物
SG11201703693UA (en) 2014-11-11 2017-06-29 Illumina Cambridge Ltd Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
DK3218513T3 (en) 2014-11-11 2019-02-04 Illumina Inc Polynucleotide amplification using CRISPR-CAS systems
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
US11789906B2 (en) 2014-11-19 2023-10-17 Arc Bio, Llc Systems and methods for genomic manipulations and analysis
US11066705B2 (en) 2014-11-25 2021-07-20 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
WO2016090266A1 (en) 2014-12-05 2016-06-09 Amyris, Inc. High-throughput sequencing of polynucleotides
EP3271480B8 (de) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Abtastung auf strukturelle varianten
PT3256604T (pt) 2015-02-10 2020-05-18 Illumina Inc Métodos e composições para analisar componentes celulares
CN107250383B (zh) 2015-02-17 2022-09-06 深圳华大智造科技股份有限公司 使用受控的链置换的dna序列测定
AU2016222788B2 (en) 2015-02-24 2022-03-31 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
CN107847930B (zh) 2015-03-20 2020-06-30 亿明达股份有限公司 在竖直或大致竖直的位置中使用的流体盒
EP3277839B1 (de) * 2015-03-31 2020-12-02 Illumina Cambridge Limited Oberfläche für conkatemerization von matrizen
EP3277294A4 (de) 2015-04-01 2018-11-14 Adaptive Biotechnologies Corp. Verfahren zur identifizierung von menschlichen kompatiblen t-zell-rezeptoren spezifisch für ein antigen-ziel
EP3530752B1 (de) 2015-04-10 2021-03-24 Spatial Transcriptomics AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
WO2016183106A1 (en) 2015-05-11 2016-11-17 Natera, Inc. Methods and compositions for determining ploidy
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
WO2016196358A1 (en) 2015-05-29 2016-12-08 Epicentre Technologies Corporation Methods of analyzing nucleic acids
CA2985545C (en) 2015-05-29 2021-02-09 Illumina Cambridge Limited Enhanced utilization of surface primers in clusters
US11274333B2 (en) 2015-05-29 2022-03-15 Molecular Cloning Laboratories (MCLAB) LLC Compositions and methods for preparing sequencing libraries
EP3653728B1 (de) 2015-06-09 2023-02-01 Life Technologies Corporation Verfahren, systeme, zusammensetzungen, kits, vorrichtung und computerlesbare medien zur molekularen markierung
US10941423B2 (en) * 2015-06-26 2021-03-09 Global Bioenergies Method for the production of isoamyl alcohol
ES2877205T3 (es) 2015-07-06 2021-11-16 Illumina Cambridge Ltd Preparación de muestras para la amplificación de ácido nucleico
ES2888626T3 (es) 2015-07-27 2022-01-05 Illumina Inc Cartografiado espacial de información de secuencia de ácidos nucleicos
JP6946292B2 (ja) 2015-08-06 2021-10-06 エイアールシー バイオ リミテッド ライアビリティ カンパニー ゲノム分析のためのシステムおよび方法
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
ES2868195T3 (es) 2015-08-14 2021-10-21 Illumina Inc Sistemas y métodos que utilizan sensores magnéticamente sensibles para determinar una característica genética
CN116338219A (zh) 2015-08-24 2023-06-27 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制系统
US20200231947A1 (en) 2015-09-09 2020-07-23 Qiagen Gmbh Polymerase enzyme
DE102015011970B4 (de) 2015-09-09 2017-04-06 Scienion Ag Dispensiergerät und entsprechendes Dispensierverfahren
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
US20180247015A1 (en) * 2015-09-24 2018-08-30 Seegene, Inc. Multiple dataset analysis for determining the presence or absence of target analyte
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
WO2017062343A1 (en) 2015-10-06 2017-04-13 Pierce Biotechnology, Inc. Devices and methods for producing nucleic acids and proteins
US10577643B2 (en) 2015-10-07 2020-03-03 Illumina, Inc. Off-target capture reduction in sequencing techniques
EP3359693A4 (de) 2015-10-09 2019-03-06 Accuragen Holdings Limited Verfahren und zusammensetzungen zur anreicherung von amplifikationsprodukten
US10253352B2 (en) 2015-11-17 2019-04-09 Omniome, Inc. Methods for determining sequence profiles
WO2017096322A1 (en) 2015-12-03 2017-06-08 Accuragen Holdings Limited Methods and compositions for forming ligation products
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
EP3858996B1 (de) 2015-12-07 2022-08-03 Zymergen Inc. Verbesserung eines mikrobiellen stammes durch eine htp-genom-engineering-plattform
CA3007635A1 (en) 2015-12-07 2017-06-15 Zymergen Inc. Promoters from corynebacterium glutamicum
PL3387152T3 (pl) * 2015-12-08 2022-05-09 Twinstrand Biosciences, Inc. Ulepszone adaptory, sposoby i kompozycje do sekwencjonowania dupleksowego
EP3433373B1 (de) 2016-03-22 2022-01-12 Myriad Women's Health, Inc. Kombinatorisches dna-screening
WO2017180909A1 (en) 2016-04-13 2017-10-19 Nextgen Jane, Inc. Sample collection and preservation devices, systems and methods
EP3235905A1 (de) 2016-04-20 2017-10-25 QIAGEN GmbH Verfahren zur erzeugung einer strang-rna-bibliothek
CN116397007A (zh) 2016-05-11 2023-07-07 伊鲁米那股份有限公司 使用argonaute系统的多核苷酸富集和扩增
CA3024630A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
EP3478833A4 (de) 2016-06-30 2019-10-02 Zymergen, Inc. Verfahren zum erzeugen einer bakteriellen hämoglobin-bibliothek und deren verwendung
JP2019519241A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド グルコース透過酵素ライブラリーを生成するための方法およびその使用
CN109790575A (zh) * 2016-07-20 2019-05-21 吉纳普赛斯股份有限公司 用于核酸测序的系统和方法
JP7155021B2 (ja) 2016-07-22 2022-10-18 オレゴン ヘルス アンド サイエンス ユニヴァーシティ 単細胞全ゲノムライブラリおよびそれを作成する組み合わせインデックス付加方法
WO2018035170A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
EP3500082A1 (de) 2016-08-22 2019-06-26 Biolumic Limited System, vorrichtung und verfahren zur behandlung von saatgut
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
MX2019003344A (es) 2016-09-22 2019-09-04 Illumina Inc Deteccion de variacion de numero de copias somaticas.
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
IL290157B2 (en) 2016-10-14 2023-09-01 Illumina Inc cartridge kit
DE102016120124B8 (de) 2016-10-21 2018-08-23 Gna Biosolutions Gmbh Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens
KR102534408B1 (ko) 2016-11-16 2023-05-18 카탈로그 테크놀로지스, 인크. 핵산-기반 데이터 저장
US10650312B2 (en) 2016-11-16 2020-05-12 Catalog Technologies, Inc. Nucleic acid-based data storage
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
CA3025880C (en) 2016-12-22 2020-01-28 Illumina Cambridge Limited Coumarin compounds and their uses as fluorescent labels
US11150179B2 (en) 2017-01-06 2021-10-19 Illumina, Inc. Phasing correction
KR102326612B1 (ko) 2017-01-17 2021-11-15 일루미나, 인코포레이티드 종양원성 스플라이스 변이체 결정
WO2018136248A1 (en) 2017-01-18 2018-07-26 Illuminia, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
EP3565900B1 (de) 2017-01-20 2021-04-07 Omniome, Inc. Genotypisierung durch polymerasebindung
SG11201906567YA (en) 2017-01-20 2019-08-27 Omniome Inc Allele-specific capture of nucleic acids
WO2018144216A1 (en) 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
US10752946B2 (en) 2017-01-31 2020-08-25 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
EP3580351A1 (de) 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymeraseenzym aus 9°n
WO2018148727A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from 9°n
WO2018148723A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus abyssi
WO2018148724A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus furiosus
WO2018148726A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from phage t4
SG10201912969XA (en) 2017-02-28 2020-02-27 Univ Pennsylvania Adeno-associated virus (aav) clade f vector and uses therefor
JOP20190200A1 (ar) 2017-02-28 2019-08-27 Univ Pennsylvania تركيبات نافعة في معالجة ضمور العضل النخاعي
US20200377935A1 (en) 2017-03-24 2020-12-03 Life Technologies Corporation Polynucleotide adapters and methods of use thereof
US11584958B2 (en) 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
EP3615692A1 (de) 2017-04-23 2020-03-04 Illumina Cambridge Limited Zusammensetzungen und verfahren zur verbesserung der probenidentifikation in indizierten nukleinsäurebibliotheken
EP3913053A1 (de) 2017-04-23 2021-11-24 Illumina Cambridge Limited Zusammensetzungen und verfahren zur verbesserung der probenidentifikation in indizierten nukleinsäurebibliotheken
ES2929837T3 (es) 2017-04-23 2022-12-02 Illumina Cambridge Ltd Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácidos nucleicos indexados
WO2018200386A1 (en) 2017-04-23 2018-11-01 Illumina, Inc. Compositions and methods for improving sample identification in indexed nucleic acid libraries
US11028435B2 (en) 2017-05-01 2021-06-08 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
AU2018266377A1 (en) 2017-05-08 2019-11-14 Illumina, Inc. Universal short adapters for indexing of polynucleotide samples
CA3061655A1 (en) 2017-05-11 2018-11-15 The Trustees Of The University Of Pennsylvania Gene therapy for neuronal ceroid lipofuscinoses
WO2018213803A1 (en) 2017-05-19 2018-11-22 Neon Therapeutics, Inc. Immunogenic neoantigen identification
CN110741091A (zh) 2017-05-19 2020-01-31 齐默尔根公司 增加nadph的生物合成途径的基因组工程化
CN110719956A (zh) 2017-06-06 2020-01-21 齐默尔根公司 用于改良真菌菌株的高通量基因组工程改造平台
WO2018226893A2 (en) 2017-06-06 2018-12-13 Zymergen Inc. A high-throughput (htp) genomic engineering platform for improving saccharopolyspora spinosa
JP2020524490A (ja) 2017-06-06 2020-08-20 ザイマージェン インコーポレイテッド Ｅｓｃｈｅｒｉｃｈｉａ Ｃｏｌｉを改良するためのＨＴＰゲノム操作プラットフォーム
EP3635111A1 (de) 2017-06-06 2020-04-15 Zymergen, Inc. Transposonmutagenese mit hohem durchsatz
EP4293122A3 (de) 2017-06-07 2024-01-24 Oregon Health & Science University Einzelzellen-ganzgenombibliotheken zur methylierungssequenzierung
WO2018236631A1 (en) 2017-06-20 2018-12-27 Illumina, Inc. METHODS AND COMPOSITIONS FOR RESOLVING INEFFICIENCY IN AMPLIFICATION REACTIONS
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
WO2019027767A1 (en) 2017-07-31 2019-02-07 Illumina Inc. SEQUENCING SYSTEM COMPRISING AGGREGATION OF MULTIPLEXED BIOLOGICAL SAMPLES
CA3067048C (en) 2017-08-01 2024-03-05 Illumina, Inc. Hydrogel beads for nucleotide sequencing
US11352668B2 (en) 2017-08-01 2022-06-07 Illumina, Inc. Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
JP6998404B2 (ja) 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 標的ヌクレオチド配列の富化及び決定方法
WO2019038594A2 (en) 2017-08-21 2019-02-28 Biolumic Limited TRANSGENIC PLANTS WITH HIGH GROWTH AND HIGH RUSTICITY
SK862017A3 (sk) 2017-08-24 2020-05-04 Grendar Marian Doc Mgr Phd Spôsob použitia fetálnej frakcie a chromozómovej reprezentácie pri určovaní aneuploidného stavu v neinvazívnom prenatálnom testovaní
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
CA3076378A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
GB201716931D0 (en) 2017-10-16 2017-11-29 Illumina Cambridge Ltd New fluorescent compounds and their use as biomarkers
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
KR20230028569A (ko) 2017-11-06 2023-02-28 일루미나, 인코포레이티드 핵산 색인 기술
US11739367B2 (en) 2017-11-08 2023-08-29 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
CN114023403A (zh) 2017-11-13 2022-02-08 多发性骨髓瘤研究基金会公司 综合、分子、组学、免疫疗法、代谢、表观遗传学和临床数据库
CA3067422C (en) 2017-11-16 2023-08-29 Illumina, Inc. Systems and methods for determining microsatellite instability
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019108972A1 (en) 2017-11-30 2019-06-06 Illumina, Inc. Validation methods and systems for sequence variant calls
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
NZ759656A (en) 2018-01-08 2022-07-01 Illumina Inc Systems and devices for high-throughput sequencing with semiconductor-based detection
EP4083225A1 (de) 2018-02-13 2022-11-02 Illumina, Inc. Dna-sequenzierung unter verwendung von hydrogelperlen
KR20200132921A (ko) 2018-03-16 2020-11-25 카탈로그 테크놀로지스, 인크. 핵산-기반 데이터를 저장하기 위한 화학적 방법들
KR20200132870A (ko) 2018-03-20 2020-11-25 지머젠 인코포레이티드 중국 햄스터 난소 세포의 유전자 조작을 위한 htp 플랫폼
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
US20190367909A1 (en) 2018-04-02 2019-12-05 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
CA3095292A1 (en) 2018-04-02 2019-10-10 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
EP3553182A1 (de) 2018-04-11 2019-10-16 Université de Bourgogne Verfahren zum nachweis von somatischen genetischen anomalien, kombination von fangsonden und nachweiskit
KR20200026250A (ko) 2018-04-20 2020-03-10 일루미나, 인코포레이티드 단일 세포를 캡슐화하는 방법, 캡슐화된 세포 및 이의 용도
JP7100069B2 (ja) 2018-05-15 2022-07-12 イルミナ インコーポレイテッド 表面結合オリゴヌクレオチドを化学開裂および脱保護するための組成物および方法
KR20210029147A (ko) 2018-05-16 2021-03-15 카탈로그 테크놀로지스, 인크. 핵산-기반 데이터를 저장하기 위한 조성물 및 방법
DK3810774T3 (da) 2018-06-04 2023-12-11 Illumina Inc Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse
US11525159B2 (en) 2018-07-03 2022-12-13 Natera, Inc. Methods for detection of donor-derived cell-free DNA
EP3821004A4 (de) 2018-07-12 2022-04-20 Twinstrand Biosciences, Inc. Verfahren und reagenzien zur charakterisierung genomischer editierung, klonale expansion und zugehörige anwendungen
JP7431802B2 (ja) 2018-08-15 2024-02-15 イルミナ インコーポレイテッド ライブラリー富化を改善するための組成物および方法
US20210317524A1 (en) 2018-08-28 2021-10-14 10X Genomics, Inc. Resolving spatial arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3844300A1 (de) 2018-08-28 2021-07-07 Sophia Genetics S.A. Verfahren zur asymmetrischen dna-bibliothekserstellung und optional integrierten duplex-sequenzierung
SG11202102029TA (en) 2018-08-28 2021-03-30 10X Genomics Inc Methods for generating spatially barcoded arrays
US11242512B2 (en) 2018-09-17 2022-02-08 Omniome, Inc. Engineered polymerases for improved sequencing
CN112840023B (zh) 2018-10-25 2024-03-01 Illumina公司 使用索引和条形码识别阵列上的配体的方法和组合物
SG11202102703VA (en) 2018-10-26 2021-04-29 Illumina Inc Modulating polymer beads for dna processing
NL2022043B1 (en) 2018-11-21 2020-06-03 Akershus Univ Hf Tagmentation-Associated Multiplex PCR Enrichment Sequencing
FI3887540T3 (fi) 2018-11-30 2023-09-14 Illumina Inc Useiden analyyttien analyysi käyttäen yhtä määritystä
SG11202012762WA (en) 2018-12-05 2021-01-28 Illumina Cambridge Ltd Methods and compositions for cluster generation by bridge amplification
EP3894587A1 (de) 2018-12-10 2021-10-20 10X Genomics, Inc. Auflösung von räumlichen anordnungen durch nähebasierte dekonvolution
CN112639126A (zh) 2018-12-14 2021-04-09 伊卢米纳剑桥有限公司 降低测序期间用未标记的核苷酸的定相
AU2019411266A1 (en) 2018-12-17 2021-01-07 Illumina Cambridge Limited Compositions for use in polyunucleotide sequencing
SG11202012758WA (en) 2018-12-17 2021-01-28 Illumina Cambridge Ltd Primer oligonucleotide for sequencing
CN112739830A (zh) 2018-12-18 2021-04-30 伊卢米纳剑桥有限公司 使用单一表面引物进行配对末端测序的方法和组合物
WO2020132103A1 (en) 2018-12-19 2020-06-25 Illumina, Inc. Methods for improving polynucleotide cluster clonality priority
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020146740A1 (en) 2019-01-10 2020-07-16 Iovance Biotherapeutics, Inc. System and methods for monitoring adoptive cell therapy clonality and persistence
WO2020169635A1 (en) 2019-02-20 2020-08-27 Grendar Marian Method for determining the uncertainty of the degree of placental mosaicism of a sample in non-invasive prenatal screening
EP3931354A1 (de) 2019-02-28 2022-01-05 10X Genomics, Inc. Profilierung von biologischen analyten mit räumlich barcodierten oligonukleotid-arrays
CA3103900A1 (en) 2019-03-01 2020-09-10 Illumina Cambridge Limited Exocyclic amine substituted coumarin compounds and their uses as fluorescent labels
AU2020232618A1 (en) 2019-03-01 2021-04-08 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
BR112020026669A2 (pt) 2019-03-01 2021-09-08 Illumina Cambridge Limited Composto de cumarina substituído com amina terciária, nucleotídeos ou oligonucleotídeos, kit, uso dos mesmos, método de sequenciamento e método para sintetizar um composto
EP3938538A1 (de) 2019-03-15 2022-01-19 10X Genomics, Inc. Verfahren zur verwendung von räumlichen anordnungen für einzelzellsequenzierung
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
EP3947718A4 (de) 2019-04-02 2022-12-21 Enumera Molecular, Inc. Verfahren, systeme und zusammensetzungen zum zählen von nukleinsäuremolekülen
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
WO2020227143A1 (en) * 2019-05-03 2020-11-12 Ultima Genomics, Inc. Fast-forward sequencing by synthesis methods
WO2020227718A1 (en) 2019-05-09 2020-11-12 Catalog Technologies, Inc. Data structures and operations for searching, computing, and indexing in dna-based data storage
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US20220154173A1 (en) 2019-07-12 2022-05-19 Iiiumina Cambridge Limited Compositions and Methods for Preparing Nucleic Acid Sequencing Libraries Using CRISPR/CAS9 Immobilized on a Solid Support
BR112021026562A2 (pt) 2019-09-20 2022-11-29 Illumina Inc Métodos e composições para identificar ligantes em arranjos com o uso de índices e códigos de barras
US11111507B2 (en) 2019-09-23 2021-09-07 Zymergen Inc. Method for counterselection in microorganisms
EP4038546A1 (de) 2019-10-01 2022-08-10 10X Genomics, Inc. Systeme und verfahren zur identifizierung von morphologischen mustern in gewebeproben
US11535842B2 (en) 2019-10-11 2022-12-27 Catalog Technologies, Inc. Nucleic acid security and authentication
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
EP4055155A1 (de) 2019-11-08 2022-09-14 Pacific Biosciences of California, Inc. Modifizierte polymerasen zur verbesserten sequenzierung durch bindung
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
US20210155982A1 (en) 2019-11-21 2021-05-27 10X Genomics, Inc. Pipeline for spatial analysis of analytes
CN114829369A (zh) 2019-11-27 2022-07-29 伊鲁米纳剑桥有限公司 含环辛四烯的染料和组合物
BR112021019640A2 (pt) 2019-12-19 2022-06-21 Illumina Inc Bibliotecas de células únicas de alto rendimento e métodos de preparo e uso
EP3891300B1 (de) 2019-12-23 2023-03-29 10X Genomics, Inc. Verfahren zur räumlichen analyse mittels rna-template-ligation
US20210189483A1 (en) 2019-12-23 2021-06-24 Mgi Tech Co. Ltd. Controlled strand-displacement for paired end sequencing
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
IL294909A (en) 2020-02-13 2022-09-01 Zymergen Inc A metagenomic library and natural product discovery platform
CN115485391A (zh) 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
AU2021225020A1 (en) 2020-02-21 2022-08-18 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
WO2021180724A1 (en) 2020-03-09 2021-09-16 Illumina, Inc. Improvements in nucleic acid sequencing
KR20220151101A (ko) 2020-03-09 2022-11-14 일루미나, 인코포레이티드 폴리뉴클레오티드를 서열분석하는 방법
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US20230207059A1 (en) 2020-05-08 2023-06-29 Illumina, Inc. Genome sequencing and detection techniques
WO2021231493A1 (en) 2020-05-11 2021-11-18 Catalog Technologies, Inc. Programs and functions in dna-based data storage
KR20230035237A (ko) 2020-05-12 2023-03-13 일루미나, 인코포레이티드 재조합 말단 데옥시뉴클레오티딜 트랜스퍼라제를 사용하여 변형된 염기를 갖는 핵산의 생성
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
WO2021247568A1 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Spatial trancriptomics for antigen-receptors
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
EP4162074B1 (de) 2020-06-08 2024-04-24 10X Genomics, Inc. Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
CN115867560A (zh) 2020-06-22 2023-03-28 伊鲁米纳剑桥有限公司 具有3’缩醛封端基团的核苷与核苷酸
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
AU2021299216A1 (en) 2020-06-30 2022-12-08 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless DNA
IL299052A (en) 2020-07-02 2023-02-01 Illumina Inc A method for calibrating the seeding efficiency of a nucleic acid library in flow cells
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
MX2023000345A (es) 2020-07-08 2023-02-13 Illumina Inc Globulos como portadores de transposomas.
US20220033900A1 (en) 2020-07-28 2022-02-03 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
EP4192951A1 (de) 2020-08-06 2023-06-14 Illumina, Inc. Herstellung von rna- und dna-sequenzierungsbibliotheken mit perlgebundenen transposomen
JP2023537850A (ja) 2020-08-18 2023-09-06 イルミナ インコーポレイテッド 核酸の配列特異的標的化転位並びに選択及び選別
JP2023540177A (ja) 2020-09-11 2023-09-22 イルミナ ケンブリッジ リミテッド ヘアピンアダプターを使用して配列決定ライブラリーから標的配列を濃縮する方法
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
CN116438319A (zh) 2020-10-21 2023-07-14 Illumina公司 包含多个插入物的测序模板以及用于提高测序通量的组合物和方法
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US20220170010A1 (en) 2020-12-02 2022-06-02 Illumina Software, Inc. System and method for detection of genetic alterations
EP4012046A1 (de) 2020-12-11 2022-06-15 10X Genomics, Inc. Verfahren und zusammensetzungen zur multimodalen in-situ-analyse
US20220195196A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Alkylpyridinium coumarin dyes and uses in sequencing applications
US20220195517A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Long stokes shift chromenoquinoline dyes and uses in sequencing applications
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
US20220228200A1 (en) 2021-01-19 2022-07-21 10X Genomics, Inc. Methods and compositions for internally controlled in situ assays
US20220235403A1 (en) 2021-01-26 2022-07-28 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
WO2022165188A1 (en) 2021-01-29 2022-08-04 Illumina, Inc. Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides
EP4284335A1 (de) 2021-02-01 2023-12-06 RegenxBio Inc. Gentherapie für neuronale ceroid-lipofuscinosen
JP2024506304A (ja) 2021-02-04 2024-02-13 イルミナ インコーポレイテッド トランスポソーム結合ビーズ上でのロングインデックス付き連結リード生成
US20220282319A1 (en) 2021-03-03 2022-09-08 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
EP4301870A1 (de) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
CN117222747A (zh) 2021-03-22 2023-12-12 伊鲁米纳剑桥有限公司 用于改善核酸簇克隆性的方法
JP2024514430A (ja) 2021-03-24 2024-04-02 カタログ テクノロジーズ， インコーポレイテッド 固定小数点数表現及び計算回路
IL307177A (en) 2021-03-29 2023-11-01 Illumina Inc Compounds and methods for assessing DNA damage in the library and normalizing amplicon size bias
CA3214278A1 (en) 2021-03-29 2022-10-06 Illumina, Inc Improved methods of library preparation
IL307172A (en) 2021-03-30 2023-11-01 Illumina Inc Improved methods of isothermal complementary DNA and library preparation
KR20230164668A (ko) 2021-03-31 2023-12-04 일루미나, 인코포레이티드 오류 수정을 위한 고유 분자 식별자를 이용한 트랜스포존-기반 기술을 사용하는 방향성 태그먼트화 시퀀싱 라이브러리 제조 방법
KR20230165273A (ko) 2021-03-31 2023-12-05 일루미나 케임브리지 리미티드 어댑터 이량체 검출을 갖는 핵산 라이브러리 서열분석 기술
BR112023019465A2 (pt) 2021-04-02 2023-12-05 Illumina Software Inc Modelo de aprendizado de máquina para detectar uma bolha dentro de uma lâmina de amostra de nucleotídeo para sequenciamento
AU2022264021A1 (en) 2021-04-29 2023-11-09 Illumina Cambridge Limited Amplification techniques for nucleic acid characterization
AU2022269804A1 (en) 2021-05-05 2023-11-02 Illumina Cambridge Limited Fluorescent dyes containing bis-boron fused heterocycles and uses in sequencing
US20220396832A1 (en) 2021-05-20 2022-12-15 Illumina Cambridge Limited Compositions and methods for sequencing by synthesis
CN113293205A (zh) 2021-05-24 2021-08-24 深圳市真迈生物科技有限公司 测序方法
WO2022256324A1 (en) 2021-06-01 2022-12-08 10X Genomics, Inc. Methods and compositions for analyte detection and probe resolution
WO2022256422A1 (en) 2021-06-02 2022-12-08 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
US20220403450A1 (en) 2021-06-03 2022-12-22 Illumina Software, Inc. Systems and methods for sequencing nucleotides using two optical channels
US11236388B1 (en) 2021-06-17 2022-02-01 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
EP4359555A1 (de) 2021-06-23 2024-05-01 Illumina, Inc. Zusammensetzungen, verfahren, kits, kartuschen und systeme zur sequenzierung von reagenzien
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
US20220415442A1 (en) 2021-06-29 2022-12-29 Illumina Software, Inc. Signal-to-noise-ratio metric for determining nucleotide-base calls and base-call quality
KR20240027599A (ko) 2021-06-29 2024-03-04 일루미나, 인코포레이티드 올리고 서열을 사용하여 훈련된 자체-학습 염기 호출자
US20220415443A1 (en) 2021-06-29 2022-12-29 Illumina, Inc. Machine-learning model for generating confidence classifications for genomic coordinates
US20230005253A1 (en) 2021-07-01 2023-01-05 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
WO2023287617A1 (en) 2021-07-13 2023-01-19 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
US20230026886A1 (en) 2021-07-13 2023-01-26 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
KR20240031968A (ko) 2021-07-19 2024-03-08 일루미나, 인코포레이티드 염기 호출에 대한 보간 및 적응을 갖는 강도 추출
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
IL309786A (en) 2021-07-28 2024-02-01 Illumina Inc Quality score calibration of BASECALLING systems
US20230057571A1 (en) 2021-08-03 2023-02-23 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
KR20240035413A (ko) 2021-08-03 2024-03-15 일루미나, 인코포레이티드 다중 염기 호출자 모델을 사용한 염기 호출
CN117858958A (zh) 2021-08-16 2024-04-09 10X基因组学有限公司 包含分割条形码区的探针和使用方法
EP4196605A1 (de) 2021-09-01 2023-06-21 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur blockierung einer erfassungssonde auf einer räumlichen anordnung
WO2023034079A1 (en) 2021-09-01 2023-03-09 Illumina Software, Inc. Amplitude modulation for accelerated base calling
WO2023044229A1 (en) 2021-09-17 2023-03-23 Illumina, Inc. Automatically identifying failure sources in nucleotide sequencing from base-call-error patterns
US20230095961A1 (en) 2021-09-21 2023-03-30 Illumina, Inc. Graph reference genome and base-calling approach using imputed haplotypes
WO2023056328A2 (en) 2021-09-30 2023-04-06 Illumina, Inc. Solid supports and methods for depleting and/or enriching library fragments prepared from biosamples
US20230096386A1 (en) 2021-09-30 2023-03-30 Illumina Cambridge Limited Polynucleotide sequencing
WO2023091683A1 (en) 2021-11-19 2023-05-25 Catalog Technologies, Inc. Nucleic acid storage for blockchain and non-fungible tokens
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
CN117581303A (zh) 2021-12-02 2024-02-20 因美纳有限公司 产生用于确定核苷酸碱基检出的簇特异性信号校正
US20230183799A1 (en) 2021-12-10 2023-06-15 Illumina, Inc. Parallel sample and index sequencing
WO2023114397A1 (en) 2021-12-16 2023-06-22 Illumina, Inc. Hybrid clustering
CN117813390A (zh) 2021-12-16 2024-04-02 因美纳有限公司 用于表面结合的多核苷酸的金属定向裂解的方法
AU2022413263A1 (en) 2021-12-17 2024-01-18 Illumina, Inc. Orthogonal hybridization
CN117813399A (zh) 2021-12-20 2024-04-02 因美纳有限公司 用于化学裂解表面结合的多核苷酸的高碘酸盐组合物和方法
WO2023122491A1 (en) 2021-12-20 2023-06-29 Illumina Cambridge Limited Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
CN117813397A (zh) 2021-12-21 2024-04-02 因美纳有限公司 蜡-微球基质组合物及其制备和使用方法
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
AU2022424380A1 (en) 2021-12-29 2024-01-18 Illumina, Inc. Methods of nucleic acid sequencing using surface-bound primers
WO2023129764A1 (en) 2021-12-29 2023-07-06 Illumina Software, Inc. Automatically switching variant analysis model versions for genomic analysis applications
US20230279475A1 (en) 2022-01-21 2023-09-07 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023164570A1 (en) 2022-02-23 2023-08-31 Insitro, Inc. Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
WO2023164660A1 (en) 2022-02-25 2023-08-31 Illumina, Inc. Calibration sequences for nucelotide sequencing
AU2023225949A1 (en) 2022-02-25 2024-01-18 Illumina, Inc. Machine-learning models for detecting and adjusting values for nucleotide methylation levels
WO2023168085A1 (en) 2022-03-04 2023-09-07 Catalog Technologies, Inc. Dna microarrays and component level sequencing for nucleic acid-based data storage and processing
EP4341435A1 (de) 2022-03-15 2024-03-27 Illumina, Inc. Verfahren für basenrufende nukleobasen
AU2023234670A1 (en) 2022-03-15 2024-01-18 Illumina, Inc. Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection
CA3223115A1 (en) 2022-03-28 2023-10-05 Xiaolin Wu Labeled avidin and methods for sequencing
US20230314322A1 (en) 2022-03-29 2023-10-05 Illumina, Inc. Systems and methods of sequencing polynucleotides
US20230313294A1 (en) 2022-03-30 2023-10-05 Illumina Cambridge Limited Methods for chemical cleavage of surface-bound polynucleotides
US20230332197A1 (en) 2022-03-31 2023-10-19 Illumina Singapore Pte. Ltd. Nucleosides and nucleotides with 3' vinyl blocking group
US20230357845A1 (en) 2022-03-31 2023-11-09 Illumina, Inc. Compositions and methods for improving sequencing signals
WO2023187061A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Paired-end re-synthesis using blocked p5 primers
WO2023192616A1 (en) 2022-04-01 2023-10-05 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
US20230323427A1 (en) 2022-04-06 2023-10-12 10X Genomics, Inc. Methods and compositions for multiplex cell analysis
CA3223722A1 (en) 2022-04-07 2023-10-12 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2023196528A1 (en) 2022-04-08 2023-10-12 Illumina, Inc. Aptamer dynamic range compression and detection techniques
WO2023212601A1 (en) 2022-04-26 2023-11-02 Illumina, Inc. Machine-learning models for selecting oligonucleotide probes for array technologies
WO2023209606A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
WO2023215612A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
WO2023220627A1 (en) 2022-05-10 2023-11-16 Illumina Software, Inc. Adaptive neural network for nucelotide sequencing
WO2023225095A1 (en) 2022-05-18 2023-11-23 Illumina Cambridge Limited Preparation of size-controlled nucleic acid fragments
US20230383342A1 (en) 2022-05-31 2023-11-30 Illumina Cambridge Limited Compositions and methods for nucleic acid sequencing
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2023250504A1 (en) 2022-06-24 2023-12-28 Illumina Software, Inc. Improving split-read alignment by intelligently identifying and scoring candidate split groups
US20230420075A1 (en) 2022-06-27 2023-12-28 Illumina Software, Inc. Accelerators for a genotype imputation model
US20230420082A1 (en) 2022-06-27 2023-12-28 Illumina Software, Inc. Generating and implementing a structural variation graph genome
WO2024006705A1 (en) 2022-06-27 2024-01-04 Illumina Software, Inc. Improved human leukocyte antigen (hla) genotyping
US20230416279A1 (en) 2022-06-28 2023-12-28 Illumina Cambridge Limited Fluorescent dyes containing fused tetracyclic bis-boron heterocycle and uses in sequencing
WO2024026356A1 (en) 2022-07-26 2024-02-01 Illumina, Inc. Rapid single-cell multiomics processing using an executable file
WO2024036304A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Puma1 polymerases and uses thereof
US20240084373A1 (en) 2022-08-16 2024-03-14 10X Genomics, Inc. Ap50 polymerases and uses thereof
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024059852A1 (en) 2022-09-16 2024-03-21 Illumina, Inc. Cluster segmentation and conditional base calling
WO2024061799A1 (en) 2022-09-19 2024-03-28 Illumina, Inc. Deformable polymers comprising immobilised primers
US20240102067A1 (en) 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods
WO2024073516A1 (en) 2022-09-29 2024-04-04 Illumina, Inc. A target-variant-reference panel for imputing target variants
US20240124914A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Thermophilic compositions for nucleic acid amplification
US20240120027A1 (en) 2022-09-30 2024-04-11 Illumina, Inc. Machine-learning model for refining structural variant calls
US20240110221A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
US20240110234A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Amplification Compositions and Methods
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
US20240124929A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Mesophilic compositions for nucleic acid amplification
WO2024068889A2 (en) 2022-09-30 2024-04-04 Illumina, Inc. Compositions and methods for reducing photo damage during sequencing
US20240127905A1 (en) 2022-10-05 2024-04-18 Illumina, Inc. Integrating variant calls from multiple sequencing pipelines utilizing a machine learning architecture
WO2024081649A1 (en) 2022-10-11 2024-04-18 Illumina, Inc. Detecting and correcting methylation values from methylation sequencing assays
WO2024081869A1 (en) 2022-10-14 2024-04-18 10X Genomics, Inc. Methods for analysis of biological samples

Family Cites Families (107)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4957858A (en) 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
EP0224126A3 (de) 1985-11-25 1989-02-01 The University of Calgary Kovalent gekoppelte komplementäre Oligodeoxynukleotide zur Verwendung als "Primer Linkers" bei Sequenzierung von Nukleinsäuren
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1340843C (en) 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
SE8801070D0 (sv) 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US6107023A (en) 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
GB8818020D0 (en) 1988-07-28 1988-09-01 Ici Plc Method for amplification of nucleotide sequences
WO1990002205A1 (en) 1988-08-25 1990-03-08 Angenics, Inc. Detection of nucleic acid sequences using particle agglutination
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
GB8827160D0 (en) 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
CA2004326A1 (en) 1988-12-23 1990-06-23 Nanibushan Dattagupta Assay of sequences using amplified genes
IE66572B1 (en) 1989-02-13 1996-01-24 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
KR920700360A (ko) 1989-03-22 1992-02-19 하리크 프리드리히 미끄럼 베어링
US5629158A (en) 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
GB2233654A (en) 1989-07-07 1991-01-16 Nat Res Dev Immobilised polynucleotides
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1991006678A1 (en) * 1989-10-26 1991-05-16 Sri International Dna sequencing
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5326692B1 (en) * 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
US5645994A (en) 1990-07-05 1997-07-08 University Of Utah Research Foundation Method and compositions for identification of species in a sample using type II topoisomerase sequences
GB9019512D0 (en) 1990-09-06 1990-10-24 Ici Plc Assay method
CA2055755A1 (en) 1990-11-22 1992-05-23 Toshihiko Kishimoto Method of immobilizing single-stranded dna on carrier at terminal
JPH06509473A (ja) 1991-08-10 1994-10-27 メディカル・リサーチ・カウンシル 細胞個体群の処理
WO1993004199A2 (en) * 1991-08-20 1993-03-04 Scientific Generics Limited Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
EP0543484B1 (de) 1991-08-30 2001-01-31 Research Development Corporation of Japan Verfahren zur Amplifikation von DNA
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
EP0969102B1 (de) 1991-09-24 2007-12-26 Keygene N.V. Primer, Sätze und Restriktions Fragmente und dessen Benutzung in selektive Restriktionsfragmentenamplifikation
WO1993009245A1 (en) 1991-10-31 1993-05-13 University Of Pittsburgh Reverse dot blot hybridization using tandem head-to-tail monomers containing probes synthesized by staggered complementary primers
WO1993009250A1 (en) 1991-11-01 1993-05-13 Adelaide Children's Hospital Solid phase amplification process
DE4141178A1 (de) 1991-12-13 1993-06-17 Europ Lab Molekularbiolog Verfahren zur sequenzierung von nukleinsaeuren
EP1382386A3 (de) 1992-02-19 2004-12-01 The Public Health Research Institute Of The City Of New York, Inc. Neue Ordnungen für Oligonukleotide und ihr Nutzen zum Sortieren, Isolieren, Sequenzierung und Manipulieren von Nukleinsäuren
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
GB9214873D0 (en) 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
WO1994002634A1 (en) 1992-07-24 1994-02-03 University Of South Australia Amplification and detection process
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
RU2048522C1 (ru) 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
US5795714A (en) * 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
ES2176233T3 (es) * 1992-12-04 2002-12-01 Univ Yale Deteccion diagnostica amplificada con ribozimas.
US5432065A (en) 1993-03-30 1995-07-11 United States Biochemical Corporation Cycle sequencing with non-thermostable DNA polymerases
US5462854A (en) 1993-04-19 1995-10-31 Beckman Instruments, Inc. Inverse linkage oligonucleotides for chemical and enzymatic processes
WO1994025603A1 (de) 1993-05-05 1994-11-10 Boehringer Mannheim Gmbh Cholesterinoxidase aus brevibacterium sterolicum
US5514539A (en) * 1993-06-29 1996-05-07 The United States Of America As Represented By The Department Of Health And Human Services Nucleotide and deduced amino acid sequences of the envelope 1 gene of 51 isolates of hepatitis C virus and the use of reagents derived from these sequences in diagnostic methods and vaccines
WO1995009174A1 (en) 1993-09-29 1995-04-06 Miami Children's Hospital Research Institute, Inc. Aspartoacylase gene, protein, and methods of screening for mutations associated with canavan disease
US5645801A (en) 1993-10-21 1997-07-08 Abbott Laboratories Device and method for amplifying and detecting target nucleic acids
CZ122696A3 (en) 1993-11-02 1996-12-11 Neurobiological Technologies The use of crf corticotropin releasing factor or analogs thereof for preparing a therapeutical preparation
JPH07203998A (ja) * 1994-01-26 1995-08-08 Hamamatsu Photonics Kk 核酸塩基配列決定方法
AU2187295A (en) * 1994-03-18 1995-10-09 General Hospital Corporation, The Cleaved amplified rflp detection methods
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US6689561B1 (en) 1994-04-14 2004-02-10 The Regents Of The University Of California Tumor suppressor gene and methods for detection of cancer, monitoring of tumor progression and cancer treatment
CN1152946A (zh) 1994-05-28 1997-06-25 特普尼尔医学有限公司 生产核酸的拷贝
US5589330A (en) 1994-07-28 1996-12-31 Genzyme Corporation High-throughput screening method for sequence or genetic alterations in nucleic acids using elution and sequencing of complementary oligonucleotides
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US6090592A (en) 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
US6060288A (en) * 1994-08-03 2000-05-09 Mosaic Technologies Method for performing amplification of nucleic acid on supports
US6468751B1 (en) 1994-08-03 2002-10-22 Mosaic Technologies, Inc. Method and apparatus for performing amplification of nucleic acid on supports
JP3612092B2 (ja) 1994-09-07 2005-01-19 株式会社日立製作所 Ｄｎａの分離・分取法及びその解析法
US5843660A (en) 1994-09-30 1998-12-01 Promega Corporation Multiplex amplification of short tandem repeat loci
FR2726286B1 (fr) 1994-10-28 1997-01-17 Genset Sa Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede
US5512441A (en) * 1994-11-15 1996-04-30 American Health Foundation Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method
EP0804618B1 (de) 1994-11-28 1999-01-27 E.I. Du Pont De Nemours And Company Mikrosatelliteverbindung für detektion genetisches polymorphismen
AU4701396A (en) 1995-02-07 1996-08-27 Mosaic Technologies Diagnostics based on dna duplex repair
WO1996027025A1 (en) * 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US5707807A (en) 1995-03-28 1998-01-13 Research Development Corporation Of Japan Molecular indexing for expressed gene analysis
JPH11503611A (ja) * 1995-04-11 1999-03-30 トラスティーズ・オブ・ボストン・ユニバーシティ 生体高分子の固相配列決定法
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
DE19515552A1 (de) * 1995-04-27 1996-10-31 Europ Lab Molekularbiolog Simultane Sequenzierung von Nukleinsäuren
AU5797696A (en) * 1995-05-19 1996-11-29 Ely Michael Rabani Parallel multiplex polynucleotide sequencing
US5753439A (en) 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods
US5690894A (en) 1995-05-23 1997-11-25 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
SE9504099D0 (sv) 1995-11-16 1995-11-16 Pharmacia Biotech Ab A method of sequencing
ATE199572T1 (de) 1995-11-21 2001-03-15 Univ Yale Unimolekulare segmentamplifikation und bestimmung
WO1997029211A1 (en) 1996-02-09 1997-08-14 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services RESTRICTION DISPLAY (RD-PCR) OF DIFFERENTIALLY EXPRESSED mRNAs
US5759780A (en) 1996-03-29 1998-06-02 Pathogenesis Corporation Methods for enriching target nucleic acid sequences
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
US5955268A (en) 1996-04-26 1999-09-21 Abbott Laboratories Method and reagent for detecting multiple nucleic acid sequences in a test sample
EP0912761A4 (de) 1996-05-29 2004-06-09 Cornell Res Foundation Inc Bestimmung von unterschieden in der nukleisäuresequenz mittels einer kombination von ligasebestimmung und polymerase-kettenreaktion
US6203978B1 (en) 1996-05-31 2001-03-20 Du Vergier Ltd. Capture of single stranded nucleic acids
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5837466A (en) * 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
US5876936A (en) 1997-01-15 1999-03-02 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators
US6309824B1 (en) 1997-01-16 2001-10-30 Hyseq, Inc. Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes
AU6846798A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid sequencing
EP3034626A1 (de) 1997-04-01 2016-06-22 Illumina Cambridge Limited Verfahren zur vervielfältigung von nukleinsäuren
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
CA2256563A1 (en) 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
ATE358177T1 (de) 1997-07-07 2007-04-15 Medical Res Council Ein in vitro sortierverfahren
US6326489B1 (en) 1997-08-05 2001-12-04 Howard Hughes Medical Institute Surface-bound, bimolecular, double-stranded DNA arrays
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
US6248558B1 (en) 1998-03-31 2001-06-19 Vanderbilt University Sequence and method for genetic engineering of proteins with cell membrane translocating activity
US5928875A (en) 1998-05-27 1999-07-27 Betzdearborn Inc. Primers for the detection of spore forming bacteria
EP1098996A1 (de) 1998-07-20 2001-05-16 Yale University Verfahren zum nachweis von nukleinsäuren unter verwendung zielgerichteter ligation von zweiteiligen primern
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
WO2000041524A2 (en) 1999-01-11 2000-07-20 President And Fellows Of Harvard College Isothermal amplification of dna
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US20020119448A1 (en) 1999-06-23 2002-08-29 Joseph A. Sorge Methods of enriching for and identifying polymorphisms
US6107061A (en) 1999-09-18 2000-08-22 The Perkin-Elmer Corporation Modified primer extension reactions for polynucleotide sequence detection
US7078225B2 (en) 2000-06-02 2006-07-18 Nippon Shokubai Co., Ltd. Method for enzymatically producing an optically active cyanohydrin
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
CA2669728C (en) 2006-11-15 2017-04-11 Biospherex Llc Multitag sequencing and ecogenomics analysis
US7792820B2 (en) 2007-09-25 2010-09-07 International Business Machines Corporation System for intelligent consumer earcons

Also Published As

Publication number Publication date
HK1155784A1 (zh) 2012-05-25
EP1591541A3 (de) 2006-07-05
US8143008B2 (en) 2012-03-27
US20150133320A1 (en) 2015-05-14
EP1591541A2 (de) 2005-11-02
EP0972081A1 (de) 2000-01-19
EP2327797A1 (de) 2011-06-01
DE69837913T2 (de) 2008-02-07
US20110045541A1 (en) 2011-02-24
US20130231254A1 (en) 2013-09-05
US20080286795A1 (en) 2008-11-20
AU6846698A (en) 1998-10-22
ES2563643T3 (es) 2016-03-15
EP3034626A1 (de) 2016-06-22
ATE545710T1 (de) 2012-03-15
WO1998044151A1 (en) 1998-10-08
ATE364718T1 (de) 2007-07-15
US9593328B2 (en) 2017-03-14
EP2327797B1 (de) 2015-11-25
US8993271B2 (en) 2015-03-31
JP2002503954A (ja) 2002-02-05
EP1591541B1 (de) 2012-02-15
US8476044B2 (en) 2013-07-02
US9902951B2 (en) 2018-02-27
US20050100900A1 (en) 2005-05-12
US20130217586A1 (en) 2013-08-22
US20140371100A1 (en) 2014-12-18
EP0972081B1 (de) 2007-06-13
US7985565B2 (en) 2011-07-26
US20150087531A1 (en) 2015-03-26

Similar Documents

Publication Publication Date Title
DE69837913D1 (de) Verfahren zur vervielfältigung von nukleinsäure
ATE269908T1 (de) Methode zur sequenzierung von nukleinsäuren
ATE397092T1 (de) Methode zur direkten sequenzierung von nukleinsäuren
ATE356222T1 (de) Massives parallelverfahren zur dekodierung von dna und rna
DE69433487D1 (de) Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung
ATE255168T1 (de) Massenspektroskopische verfahren zur sequenzierung von nukleinsäuren
DE3687287T2 (de) Verfahren zur amplifikation von nukleinsaeuresequenzen.
ATE262595T1 (de) Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen
WO2001071042A3 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
DE69736667D1 (de) Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm)
DE69839235D1 (de) Fluorometrisches Verfahren zur Überwachung und zum Nachweis von Nukleinsäure- Amplifizierung
ATE386819T1 (de) Verfahren zur analyse von aflp reaktionsmischungen unter verwendung von primer verlängerungstechniken
DE59410068D1 (de) Verfahren zur spezifischen Klonierung von Nukleinsäuren
DE60125054D1 (de) Verfahren zur amplifikation von nukleinsäuren, das mittel zur strangtrennung und universelle sequenzmarker bereitstellt
ATE197481T1 (de) Verfahren und reagenzien zur typisierung von hla- genen der klasse i
DE60015210D1 (de) Oligonukleotid Primer zur effizienten reversen Transkription von Hepatitis C Virus (HCV) RNS und Verfahren zu deren Verwendung
DE60331132D1 (de) Verfahren zur Quantifizierung von Nukleinsäuren

Legal Events

Date Code Title Description
8364 No opposition during term of opposition