DE69131124D1 - Polymermatrix hoher zähigkeit und deren anwendungsverfahren - Google Patents

Polymermatrix hoher zähigkeit und deren anwendungsverfahren

Info

Publication number
DE69131124D1
DE69131124D1 DE69131124T DE69131124T DE69131124D1 DE 69131124 D1 DE69131124 D1 DE 69131124D1 DE 69131124 T DE69131124 T DE 69131124T DE 69131124 T DE69131124 T DE 69131124T DE 69131124 D1 DE69131124 D1 DE 69131124D1
Authority
DE
Germany
Prior art keywords
matrix
polymer matrix
high strength
application method
strength polymer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69131124T
Other languages
English (en)
Other versions
DE69131124T2 (de
Inventor
Robert Dubrow
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of DE69131124D1 publication Critical patent/DE69131124D1/de
Publication of DE69131124T2 publication Critical patent/DE69131124T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
DE69131124T 1990-01-29 1991-01-25 Polymermatrix hoher zähigkeit und deren anwendungsverfahren Expired - Lifetime DE69131124T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/472,045 US5164055A (en) 1990-01-29 1990-01-29 High-viscosity polymer matrix and methods
PCT/US1991/000545 WO1991011709A1 (en) 1990-01-29 1991-01-25 High-viscosity polymer matrix and methods

Publications (2)

Publication Number Publication Date
DE69131124D1 true DE69131124D1 (de) 1999-05-20
DE69131124T2 DE69131124T2 (de) 1999-09-23

Family

ID=23873989

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69131124T Expired - Lifetime DE69131124T2 (de) 1990-01-29 1991-01-25 Polymermatrix hoher zähigkeit und deren anwendungsverfahren

Country Status (9)

Country Link
US (1) US5164055A (de)
EP (1) EP0513164B1 (de)
JP (1) JP2701091B2 (de)
AT (1) ATE178990T1 (de)
DE (1) DE69131124T2 (de)
DK (1) DK0513164T3 (de)
ES (1) ES2132084T3 (de)
GR (1) GR3030209T3 (de)
WO (1) WO1991011709A1 (de)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665216A (en) * 1986-10-21 1997-09-09 Northeastern University Capillary column for high performance electrophoretic separation and detection of SDS proteins and system and using the same
US5332481A (en) * 1991-01-29 1994-07-26 Beckman Instruments, Inc. Capillary electrophoresis using replaceable gels
US5126021A (en) * 1991-07-17 1992-06-30 Applied Biosystems Inc. Low-viscosity polymer solution for capillary electrophoresis
US5213669A (en) * 1992-01-31 1993-05-25 Beckman Instruments, Inc. Capillary column containing a dynamically cross-linked composition and method of use
DE69318008T2 (de) * 1992-01-31 1998-08-20 Beckman Instruments Inc Kapillarsaeule mit einer entfernbaren trenngelzusammensetzung und verfahren zum gebrauch
US5759369A (en) * 1992-09-24 1998-06-02 The Perkin-Elmer Corporation Viscous electrophoresis polymer medium and method
US5290418A (en) * 1992-09-24 1994-03-01 Applied Biosystems, Inc. Viscous electrophoresis polymer medium and method
US5569364A (en) * 1992-11-05 1996-10-29 Soane Biosciences, Inc. Separation media for electrophoresis
JP3426236B2 (ja) * 1992-11-05 2003-07-14 ソーン バイオサイエンシス インコーポレイテッド 電気泳動のための分離媒体
US5891313A (en) 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
AU1254295A (en) * 1993-12-17 1995-07-03 Perkin-Elmer Corporation, The Uncharged polymers for separation of biomolecules by capillary electrophoresis
US6355709B1 (en) * 1993-12-17 2002-03-12 The Perkin-Elmer Corporation Polymers for separation of biomolecules by capillary electrophoresis
US6358385B1 (en) * 1993-12-17 2002-03-19 The Perkin-Elmer Corporation Polymers for separation of biomolecules by capillary electrophoresis
US5423966A (en) * 1994-01-25 1995-06-13 Perkin-Elmer Corporation On line ion contaminant removal apparatus and method for capillary electrophoresis
EP0744024B1 (de) * 1994-02-07 2002-01-09 PE Corporation (NY) Elektroforetische fluoreszenzvorrichtung für die analyse von polynukleotiden
US6485625B1 (en) 1995-05-09 2002-11-26 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
US5534123A (en) * 1995-07-10 1996-07-09 Molecular Dynamics Denaturing separation matrix having hydroxyethyl cellulose for nucleic acid electrophoresis
US7244622B2 (en) 1996-04-03 2007-07-17 Applera Corporation Device and method for multiple analyte detection
US6001232A (en) * 1996-09-04 1999-12-14 The Research Foundation Of State University Of New York Separation medium for capillary electrophoresis
US5989399A (en) * 1996-09-04 1999-11-23 The Research Foundation Of State University Of New York Effective surface treatment for a new separation medium in electrophoresis
US5792331A (en) * 1996-12-19 1998-08-11 Dionex Corporation Preformed polymer coating process and product
WO1999017117A1 (en) * 1997-09-30 1999-04-08 Amira Medical Analytical device with capillary reagent carrier
US5958694A (en) * 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US6117326A (en) * 1998-06-26 2000-09-12 Rohm And Haas Company Capillary electrochromatography separation media
WO2000042423A1 (en) * 1999-01-12 2000-07-20 Spectrumedix Corporation Copolymers for capillary gel electrophoresis
US6372106B1 (en) * 1999-07-26 2002-04-16 Applera Corporation Capillary electrophoresis method and apparatus for reducing peak broadening associated with the establishment of an electric field
US20020012971A1 (en) * 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
US6770698B1 (en) * 2000-06-05 2004-08-03 The Research Foundation At State University Of New York Polymer solution for separation of charged macromolecules by electrophoresis
US6706162B1 (en) 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis
AUPR161400A0 (en) * 2000-11-22 2000-12-14 Life Therapeutics Limited Apparatus and method for isoelectric focusing separations
US7347922B2 (en) * 2001-03-28 2008-03-25 Akins Jr Robert E Multi-dimensional proteomic analysis method
US7014744B2 (en) * 2001-08-24 2006-03-21 Applera Corporation Method of purification and concentration using AC fields with a transfer tip
WO2003062810A1 (en) * 2002-01-22 2003-07-31 Board Of Regents, The University Of Texas System Electrophoretic ratchets and cyclic electrophoresis
EP1485712A2 (de) * 2002-02-26 2004-12-15 Pharmacia Corporation Sequenznachweissystem-rechner
US7001853B1 (en) 2002-08-30 2006-02-21 Caliper Life Sciences, Inc. Flow control of photo-polymerizable resin
US7083711B2 (en) * 2003-02-21 2006-08-01 Combisep, Inc. Capillary electrophoresis gel especially for separation made for single stranded nucleic acid separations
US20040167480A1 (en) * 2003-02-21 2004-08-26 Advanced Medical Optics, Inc. Administration of multiple viscoelastic solutions with a multi-compartment syringe
US20050233390A1 (en) * 2003-04-09 2005-10-20 Allen John W Device including a proteinaceous factor, a recombinant proteinaceous factor, and a nucleotide sequence encoding the proteinaceous factor
US8206974B2 (en) 2005-05-19 2012-06-26 Netbio, Inc. Ruggedized apparatus for analysis of nucleic acid and proteins
US20070014699A1 (en) 2005-06-23 2007-01-18 Beckman Coulter, Inc, Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
EP1979410B1 (de) * 2005-12-29 2012-08-22 Life Technologies Corporation Zusammensetzungen und verfahren zur verbesserung der auflösung von auf polyacrylamidgelen getrennten biomolekülen
US8500980B1 (en) * 2006-10-24 2013-08-06 Qiagen Sciences, Llc Method and apparatus for high speed genotyping
KR101530943B1 (ko) 2007-04-04 2015-06-23 네트바이오, 인코포레이티드 통합된 핵산 분석
EP2112196A1 (de) * 2008-04-25 2009-10-28 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Flüssige Zusammensetzung mit Polymerketten und Partikeln aus einem anorganischen Material in einer Flüssigkeit
WO2010147654A2 (en) 2009-06-15 2010-12-23 Netbio Inc. Improved methods for forensic dna quantitation
EP2671072B1 (de) 2011-01-31 2022-03-09 Biotype GmbH Kapillarelektrophorese von nukleinsäuren mit reduzierter artefaktdenaturierung
WO2013166297A2 (en) * 2012-05-03 2013-11-07 Medimmune, Llc Method for analyzing sample components
JP6992385B2 (ja) * 2017-10-02 2022-01-13 株式会社島津製作所 電気泳動用分離媒体、電気泳動用試薬キット、及び電気泳動方法

Also Published As

Publication number Publication date
JPH06504612A (ja) 1994-05-26
US5164055A (en) 1992-11-17
GR3030209T3 (en) 1999-08-31
DE69131124T2 (de) 1999-09-23
ATE178990T1 (de) 1999-04-15
DK0513164T3 (da) 1999-10-25
JP2701091B2 (ja) 1998-01-21
EP0513164A1 (de) 1992-11-19
WO1991011709A1 (en) 1991-08-08
ES2132084T3 (es) 1999-08-16
EP0513164A4 (de) 1994-04-20
EP0513164B1 (de) 1999-04-14

Similar Documents

Publication Publication Date Title
DE69131124T2 (de) Polymermatrix hoher zähigkeit und deren anwendungsverfahren
Clarke Simplified “disc”(polyacrylamide gel) electrophoresis
DE69103631D1 (de) Verbessertes verfahren und apparat, um biologische substanzen in lebenden zellen einzuführen.
CA2153991A1 (en) High resolution dna sequencing method using low viscosity medium
DE69220132T2 (de) Anionenaustauschtrennung von Nukleinsäuren
ES2071706T3 (es) Metodo para fraccionar proteinas plasmaticas.
EP0196790A3 (de) Verfahren und Apparat zum Extrahieren von DNS aus Proben
DK461781A (da) Derivatiseret agarose og fremgangsmaade til adskillelse af biolofiske stofblandinger ved gelelectrophorese under anvendelse af denne som gelmatrix
Shepherd et al. High-resolution disc electrophoresis of histones: I. An improved method
Sudor et al. Electromigration behavior of polysaccharides in capillary electrophoresis under pulsed-field conditions.
Van der Rest et al. Separation of collagen cyanogen bromide-derived peptides by reversed-phase high-performance liquid chromatography
Ketai et al. Separation and determination of alantolactone and isoalantolactone in traditional Chinese herbs by capillary electrophoresis
Al‐Hakim et al. Isolation and recovery of acidic oligosaccharides from polyacrylamide gels by semi‐dry electrotransfer
Meza et al. Chemical heterogeneity of protofilaments forming the outer doublets from sea urchin flagella
Edström Nucleotide analysis on the cyto-scale
DK421089D0 (da) Fremgangsmaade og anordning til elektroforetisk adskillelse, rensning og berigelse af ladede eller polariserbare makromolekyler
BRPI0406265B8 (pt) método para análise por eletroforese capilar, método para pesquisa e tipagem de proteínas monoclonais, método de preparação de anticorpos modificados sobrecarregados negativamente e kit para detecção e tipagem de proteínas monoclonais
ATE191568T1 (de) Spezifische bindungssubstanzen für antikörper und deren verwendung für immunoassays oder vakzine
DE60325976D1 (de) Abreicherung von plasmaproteinen
CA2448728C (en) Electrophoresis methods
CN1333294A (zh) 一种新的提取纯化羊胎素工艺和检测其生物活性的方法
Vilim et al. Electrophoretic separation of large proteoglycans in large-pore polyacrylamide gradient gels (1.32–10.0% T) and a one-step procedure for simultaneous staining of proteins and proteoglycans
ATE72756T1 (de) Verfahren zur herstellung eines biologischen stoffes.
Landers et al. Reproducibility of sample separation using liquid or forced air convection thermostatted high performance capillary electrophoresis systems
AT384032B (de) Verfahren zur herstellung von polydispersen nativen pseudomonas-flagella(h)-antigenen (fag)

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: APPLERA CORP., FOSTER CITY, CALIF., US

8327 Change in the person/name/address of the patent owner

Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

8380 Miscellaneous part iii

Free format text: PFANDRECHT

8380 Miscellaneous part iii

Free format text: PFANDRECHT AUFGEHOBEN