DE69020968T2 - Kontrolle der Anhäufung von abweichenden Expressionsvektoren während der Fermentationsverfahren. - Google Patents

Kontrolle der Anhäufung von abweichenden Expressionsvektoren während der Fermentationsverfahren.

Info

Publication number
DE69020968T2
DE69020968T2 DE69020968T DE69020968T DE69020968T2 DE 69020968 T2 DE69020968 T2 DE 69020968T2 DE 69020968 T DE69020968 T DE 69020968T DE 69020968 T DE69020968 T DE 69020968T DE 69020968 T2 DE69020968 T2 DE 69020968T2
Authority
DE
Germany
Prior art keywords
accumulation
expression vectors
control
fermentation process
vectors during
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69020968T
Other languages
English (en)
Other versions
DE69020968D1 (de
Inventor
William Lawrence Muth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of DE69020968D1 publication Critical patent/DE69020968D1/de
Publication of DE69020968T2 publication Critical patent/DE69020968T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormones [GH] (Somatotropin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
DE69020968T 1989-06-26 1990-06-22 Kontrolle der Anhäufung von abweichenden Expressionsvektoren während der Fermentationsverfahren. Expired - Fee Related DE69020968T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37157989A 1989-06-26 1989-06-26

Publications (2)

Publication Number Publication Date
DE69020968D1 DE69020968D1 (de) 1995-08-24
DE69020968T2 true DE69020968T2 (de) 1995-12-21

Family

ID=23464539

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69020968T Expired - Fee Related DE69020968T2 (de) 1989-06-26 1990-06-22 Kontrolle der Anhäufung von abweichenden Expressionsvektoren während der Fermentationsverfahren.

Country Status (13)

Country Link
US (1) US5824496A (de)
EP (1) EP0405858B1 (de)
JP (1) JPH0347081A (de)
AT (1) ATE125300T1 (de)
AU (1) AU617057B2 (de)
CA (1) CA2019696A1 (de)
DE (1) DE69020968T2 (de)
DK (1) DK0405858T3 (de)
ES (1) ES2076318T3 (de)
GR (1) GR3017703T3 (de)
HU (1) HUT54415A (de)
IE (1) IE67799B1 (de)
IL (1) IL94847A0 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5395761A (en) * 1991-11-27 1995-03-07 Eli Lilly And Company Plasmid pHKY334, an expression vector for EK-BGH and host cells transformed therewith
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS55127986A (en) * 1979-03-26 1980-10-03 Agency Of Ind Science & Technol Bacteria pretreatment with antibiotic inhibiting synthesis of cell wall
US4529695A (en) * 1981-06-18 1985-07-16 Wisconsin Alumni Research Foundation Recombinant cloning vector, host for the vector, and method for using same
JPS5998685A (ja) * 1982-08-06 1984-06-07 ザ・ボ−ド・オブ・トラステイ−ズ・オブ・ザ・リ−ランド・スタンフオ−ド・ジユニア・ユニバ−シテイ 休止細胞を使用する生物的生成物の製造
EP0178764A1 (de) * 1984-08-28 1986-04-23 Genex Corporation Verfahren zur Stabilisierung eines Wirtsmikroorganismus-Expressionsvektor-Systems für die Herstellung von Proteinen in grossem Umfang

Also Published As

Publication number Publication date
IE67799B1 (en) 1996-05-01
DE69020968D1 (de) 1995-08-24
IE902289A1 (en) 1991-01-16
HU903975D0 (en) 1990-11-28
JPH0347081A (ja) 1991-02-28
GR3017703T3 (en) 1996-01-31
AU617057B2 (en) 1991-11-14
ATE125300T1 (de) 1995-08-15
DK0405858T3 (da) 1995-09-04
ES2076318T3 (es) 1995-11-01
US5824496A (en) 1998-10-20
AU5780590A (en) 1991-01-03
IL94847A0 (en) 1991-04-15
EP0405858B1 (de) 1995-07-19
HUT54415A (en) 1991-02-28
EP0405858A3 (en) 1992-04-29
CA2019696A1 (en) 1990-12-26
IE902289L (en) 1990-12-26
EP0405858A2 (de) 1991-01-02

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee