DE69016826D1 - Verfahren zur Synthese von Ribonukleinsäure (RNS). - Google Patents

Verfahren zur Synthese von Ribonukleinsäure (RNS).

Info

Publication number
DE69016826D1
DE69016826D1 DE69016826T DE69016826T DE69016826D1 DE 69016826 D1 DE69016826 D1 DE 69016826D1 DE 69016826 T DE69016826 T DE 69016826T DE 69016826 T DE69016826 T DE 69016826T DE 69016826 D1 DE69016826 D1 DE 69016826D1
Authority
DE
Germany
Prior art keywords
synthesis
ribonucleic acid
rna
target sequence
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69016826T
Other languages
English (en)
Other versions
DE69016826T2 (de
Inventor
Lawrence T Malek
Cheryl Davey
Peter Franklin Lens
Frits Wielaard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Akzo NV
Original Assignee
Akzo NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo NV filed Critical Akzo NV
Publication of DE69016826D1 publication Critical patent/DE69016826D1/de
Application granted granted Critical
Publication of DE69016826T2 publication Critical patent/DE69016826T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
DE69016826T 1989-05-10 1990-05-08 Verfahren zur Synthese von Ribonukleinsäure (RNS). Expired - Lifetime DE69016826T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL8901172 1989-05-10

Publications (2)

Publication Number Publication Date
DE69016826D1 true DE69016826D1 (de) 1995-03-23
DE69016826T2 DE69016826T2 (de) 1995-06-08

Family

ID=19854626

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69016826T Expired - Lifetime DE69016826T2 (de) 1989-05-10 1990-05-08 Verfahren zur Synthese von Ribonukleinsäure (RNS).

Country Status (13)

Country Link
US (1) US5466586A (de)
EP (1) EP0397269B1 (de)
JP (1) JP2839639B2 (de)
KR (1) KR0185376B1 (de)
AT (1) ATE118549T1 (de)
AU (1) AU641159B2 (de)
CA (1) CA2016358C (de)
DE (1) DE69016826T2 (de)
DK (1) DK0397269T3 (de)
ES (1) ES2070995T3 (de)
FI (1) FI102387B1 (de)
IE (1) IE66597B1 (de)
ZA (1) ZA903453B (de)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622820A (en) * 1988-03-10 1997-04-22 City Of Hope Method for amplification and detection of RNA and DNA sequences
GB8808892D0 (en) * 1988-04-15 1988-05-18 British Bio Technology Gene synthesis
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
CA2128891A1 (en) * 1992-01-29 1993-08-05 Cylia Keller Polynucleotide immobilized support
KR100249110B1 (ko) * 1992-05-06 2000-04-01 다니엘 엘. 캐시앙 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트
WO1994002636A1 (en) * 1992-07-28 1994-02-03 Hitachi Chemical Company Ltd. Gene detection system
US5712127A (en) 1996-04-29 1998-01-27 Genescape Inc. Subtractive amplification
DE19633427C2 (de) * 1996-08-20 2001-10-04 Hubert S Bernauer Verfahren zur Synthese von Nukleinsäuremolekülen mit zumindest teilweise vorbestimmter Nukleotidsequenz
DE19812103A1 (de) * 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
WO2002070735A2 (en) * 2001-03-07 2002-09-12 Biomerieux B.V. Method for the amplification and detection of dna using a transcription based amplification
EP1383915B1 (de) * 2001-03-07 2015-11-18 bioMerieux B.V Transkriptionsbasierendes verfahren zur amplifizierung und zum nachweis von hbv dna
WO2004059289A2 (en) * 2001-05-22 2004-07-15 Epicentre Technologies Target-dependent transcription using deletion mutants of n4 rna polymerase
US8137911B2 (en) * 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US7504215B2 (en) 2002-07-12 2009-03-17 Affymetrix, Inc. Nucleic acid labeling methods
US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
WO2004048596A2 (en) 2002-11-21 2004-06-10 Epicentre Technologies Methods for using primers that encode one strand of a double-stranded promoter
US7266295B2 (en) * 2003-04-17 2007-09-04 Intel Corporation Modular reconfigurable multi-server system and method for high-speed networking within photonic burst-switched network
US7713697B2 (en) * 2004-08-27 2010-05-11 Gen-Probe Incorporated Methods and kits for amplifying DNA
JP4861324B2 (ja) * 2004-08-27 2012-01-25 ジェン−プロウブ インコーポレイテッド 単一プライマー核酸増幅法
AU2006246975B2 (en) 2005-05-17 2011-08-11 Ozgene Pty Ltd Sequential cloning system
US8293684B2 (en) * 2006-11-29 2012-10-23 Exiqon Locked nucleic acid reagents for labelling nucleic acids
US8183359B2 (en) * 2007-03-01 2012-05-22 Gen-Probe Incorporated Kits for amplifying DNA
CA2707436C (en) * 2007-06-29 2014-01-28 Epicentre Technologies Corporation Copy dna and sense rna
WO2010126913A1 (en) 2009-04-27 2010-11-04 Gen-Probe Incorporated Methods and kits for use in the selective amplification of target sequences
WO2010148085A1 (en) 2009-06-16 2010-12-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Rna nanoparticles and methods of use
TWI518325B (zh) 2010-02-04 2016-01-21 自治醫科大學 對ａｌｋ抑制劑具有先抗性或後抗性癌症的識別、判斷和治療
CA2807552A1 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
ES2862955T3 (es) 2010-10-01 2021-10-08 Modernatx Inc Acidos nucleicos manipulados y métodos de uso de los mismos
US8710200B2 (en) 2011-03-31 2014-04-29 Moderna Therapeutics, Inc. Engineered nucleic acids encoding a modified erythropoietin and their expression
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
WO2013052523A1 (en) 2011-10-03 2013-04-11 modeRNA Therapeutics Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
CN104114572A (zh) 2011-12-16 2014-10-22 现代治疗公司 经修饰的核苷、核苷酸和核酸组合物
FR2984357B1 (fr) 2011-12-16 2016-11-18 Biomerieux Sa Procede d'amplification transcriptionnelle d'acides nucleiques reunissant des etapes de temperatures differentes
US9878056B2 (en) 2012-04-02 2018-01-30 Modernatx, Inc. Modified polynucleotides for the production of cosmetic proteins and peptides
EP2833920A2 (de) 2012-04-02 2015-02-11 Moderna Therapeutics, Inc. Modifizierte polynukleotide zur herstellung von biologischen stoffen und proteinen in zusammenhang mit erkrankungen beim menschen
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
PL2922554T3 (pl) 2012-11-26 2022-06-20 Modernatx, Inc. Na zmodyfikowany na końcach
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
WO2015006344A1 (en) * 2013-07-08 2015-01-15 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method for synthesizing selectively labeled rna
KR102536833B1 (ko) 2013-08-28 2023-05-26 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
JP6669655B2 (ja) 2013-09-17 2020-03-18 ザ ユナイテッド ステイツ オブ アメリカ， アズ リプレゼンテッド バイ ザ セクレタリー， デパートメント オブ ヘルス アンド ヒューマン サービシーズ 多機能ｒｎａナノ粒子及び使用方法
WO2015048744A2 (en) 2013-09-30 2015-04-02 Moderna Therapeutics, Inc. Polynucleotides encoding immune modulating polypeptides
EP3052521A1 (de) 2013-10-03 2016-08-10 Moderna Therapeutics, Inc. Polynukleotide zur codierung von low-density-lipoprotein-rezeptor
EP3068882A2 (de) 2013-11-13 2016-09-21 INSERM (Institut National de la Santé et de la Recherche Médicale) Kits von teilen mit nukleinsäuren mit fähigkeit zur bildung eines kissing-komplexes und verwendungen davon
US10517890B2 (en) 2014-05-06 2019-12-31 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Triggering RNA interference with RNA-DNA and DNA-RNA nanoparticles
WO2018187373A1 (en) 2017-04-03 2018-10-11 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Functionally-interdependent shape switching nucleic acid nanoparticles

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4766072A (en) * 1985-07-17 1988-08-23 Promega Corporation Vectors for in vitro production of RNA copies of either strand of a cloned DNA sequence
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
EP0359789B1 (de) * 1988-01-21 1993-08-04 Genentech, Inc. Verstärkung und nachweis von nukleinsäuresequenzen
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process

Also Published As

Publication number Publication date
ATE118549T1 (de) 1995-03-15
FI902318A0 (fi) 1990-05-09
AU641159B2 (en) 1993-09-16
US5466586A (en) 1995-11-14
ZA903453B (en) 1991-02-27
AU5487790A (en) 1990-11-15
JPH03180184A (ja) 1991-08-06
DE69016826T2 (de) 1995-06-08
EP0397269A1 (de) 1990-11-14
CA2016358C (en) 2000-11-28
EP0397269B1 (de) 1995-02-15
DK0397269T3 (da) 1995-05-29
JP2839639B2 (ja) 1998-12-16
KR0185376B1 (ko) 1999-04-01
FI102387B (fi) 1998-11-30
IE901624L (en) 1990-11-10
FI102387B1 (fi) 1998-11-30
IE66597B1 (en) 1996-01-24
CA2016358A1 (en) 1990-11-10
ES2070995T3 (es) 1995-06-16
KR900018376A (ko) 1990-12-21

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