DE60335116D1 - Verfahren zum nachweis von sequenzunterschieden - Google Patents

Verfahren zum nachweis von sequenzunterschieden

Info

Publication number
DE60335116D1
DE60335116D1 DE60335116T DE60335116T DE60335116D1 DE 60335116 D1 DE60335116 D1 DE 60335116D1 DE 60335116 T DE60335116 T DE 60335116T DE 60335116 T DE60335116 T DE 60335116T DE 60335116 D1 DE60335116 D1 DE 60335116D1
Authority
DE
Germany
Prior art keywords
nucleotide
identity
primer extension
genotyping
polymorphic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60335116T
Other languages
English (en)
Inventor
Vladimir I Slepnev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen Mansfield Inc
Original Assignee
PrimeraDx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PrimeraDx Inc filed Critical PrimeraDx Inc
Application granted granted Critical
Publication of DE60335116D1 publication Critical patent/DE60335116D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/125—Allele specific primer extension
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/125—Electrophoretic separation
DE60335116T 2002-06-28 2003-06-20 Verfahren zum nachweis von sequenzunterschieden Expired - Lifetime DE60335116D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39233102P 2002-06-28 2002-06-28
PCT/US2003/019576 WO2004003136A2 (en) 2002-06-28 2003-06-20 Methods of detecting sequence differences

Publications (1)

Publication Number Publication Date
DE60335116D1 true DE60335116D1 (de) 2011-01-05

Family

ID=30000848

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60335116T Expired - Lifetime DE60335116D1 (de) 2002-06-28 2003-06-20 Verfahren zum nachweis von sequenzunterschieden

Country Status (10)

Country Link
US (2) US20040096870A1 (de)
EP (1) EP1534859B1 (de)
JP (1) JP4617403B2 (de)
KR (1) KR101038137B1 (de)
CN (1) CN1665938B (de)
AT (1) ATE489477T1 (de)
AU (2) AU2003243700B2 (de)
CA (1) CA2490864C (de)
DE (1) DE60335116D1 (de)
WO (1) WO2004003136A2 (de)

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CN100400673C (zh) * 2004-03-18 2008-07-09 华晶基因技术有限公司 单核苷酸多态性（SNPs）及点突变的检测方法
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
EP1947196A4 (de) * 2005-11-08 2009-09-09 Olympus Corp Verfahren zur amplifikation mehrerer nukleinsäuresequenzen zur differenzierung
US20080020386A1 (en) * 2006-03-29 2008-01-24 Medigen Biotechnology Corporation Methods and apparatus for genotyping
FI20075124A0 (fi) * 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
WO2010146339A1 (en) * 2009-06-15 2010-12-23 Bg Research Ltd Nucleic acid detection
IT1406054B1 (it) * 2010-10-08 2014-02-06 Bios Internat S R L Metodo fluorescente per lo screening di hla-b*57:01
JP6316185B2 (ja) * 2011-05-06 2018-04-25 キアゲン ゲーエムベーハー 内部標識プライマーを含む核酸の配列決定、増幅および検出のための方法
US8809238B2 (en) * 2011-05-09 2014-08-19 Fluidigm Corporation Probe based nucleic acid detection
RS61631B1 (sr) 2012-02-17 2021-04-29 Hutchinson Fred Cancer Res Kompozicije i postupci za preciznu identifikaciju mutacija
WO2013124738A2 (en) 2012-02-21 2013-08-29 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
DE112013004650T5 (de) * 2012-09-24 2015-06-18 Cb Biotechnologies, Inc. Multiplexpyrosequenzierung unter Verwendung nichtinterferierender, rauschbeendender Polynukleotididentifikationstags
US11091758B2 (en) 2013-12-11 2021-08-17 The Regents Of The University Of California Methods for labeling DNAa fragments to reconstruct physical linkage and phase
US10526641B2 (en) 2014-08-01 2020-01-07 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
GB201414745D0 (en) 2014-08-19 2014-10-01 Articzymes As Exonucleases
JP6777966B2 (ja) 2015-02-17 2020-10-28 ダブテイル ゲノミクス エルエルシー 核酸配列アセンブリ
GB2554572B (en) 2015-03-26 2021-06-23 Dovetail Genomics Llc Physical linkage preservation in DNA storage
JP7300831B2 (ja) 2015-10-19 2023-06-30 ダブテイル ゲノミクス エルエルシー ゲノムアセンブリ、ハプロタイプフェージング、および標的に依存しない核酸検出のための方法
JP7441003B2 (ja) 2016-02-23 2024-02-29 ダブテイル ゲノミクス エルエルシー ゲノムアセンブリのためのフェージングされたリードセットの生成とハプロタイプフェージング
SG11201810088SA (en) 2016-05-13 2018-12-28 Dovetail Genomics Llc Recovering long-range linkage information from preserved samples
WO2018183942A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification

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US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
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US6001230A (en) 1988-04-29 1999-12-14 Beckman Coulter, Inc. Automated capillary electrophoresis apparatus
US5639611A (en) * 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
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Also Published As

Publication number Publication date
CN1665938B (zh) 2012-10-10
US20110300537A1 (en) 2011-12-08
ATE489477T1 (de) 2010-12-15
CA2490864A1 (en) 2004-01-08
KR101038137B1 (ko) 2011-05-31
EP1534859B1 (de) 2010-11-24
EP1534859A2 (de) 2005-06-01
CN1665938A (zh) 2005-09-07
AU2009200987A1 (en) 2009-04-09
WO2004003136A2 (en) 2004-01-08
AU2003243700A1 (en) 2004-01-19
CA2490864C (en) 2012-09-11
WO2004003136A3 (en) 2004-08-19
EP1534859A4 (de) 2007-09-05
AU2003243700B2 (en) 2009-04-30
JP2005531314A (ja) 2005-10-20
JP4617403B2 (ja) 2011-01-26
US20040096870A1 (en) 2004-05-20
KR20050034651A (ko) 2005-04-14

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