DE60312507D1 - Eine methode zur in vitro molekularen evolution der proteinfunktion - Google Patents

Eine methode zur in vitro molekularen evolution der proteinfunktion

Info

Publication number
DE60312507D1
DE60312507D1 DE60312507T DE60312507T DE60312507D1 DE 60312507 D1 DE60312507 D1 DE 60312507D1 DE 60312507 T DE60312507 T DE 60312507T DE 60312507 T DE60312507 T DE 60312507T DE 60312507 D1 DE60312507 D1 DE 60312507D1
Authority
DE
Germany
Prior art keywords
stranded polynucleotide
reaction
population
parent
polynucleotide molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60312507T
Other languages
English (en)
Other versions
DE60312507T3 (de
DE60312507T2 (de
Inventor
Christina Furebring
Roland Carlsson
Carl Arne Borrebaeck
Hager Ann-Christin Malmborg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alligator Bioscience AB
Original Assignee
Alligator Bioscience AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=29551433&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60312507(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GB0211369A external-priority patent/GB2388604B/en
Priority claimed from US10/321,195 external-priority patent/US7153655B2/en
Application filed by Alligator Bioscience AB filed Critical Alligator Bioscience AB
Application granted granted Critical
Publication of DE60312507D1 publication Critical patent/DE60312507D1/de
Publication of DE60312507T2 publication Critical patent/DE60312507T2/de
Publication of DE60312507T3 publication Critical patent/DE60312507T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
DE60312507T 2002-05-17 2003-05-16 Eine methode zur in vitro molekularen evolution der proteinfunktion Expired - Lifetime DE60312507T3 (de)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB0211369A GB2388604B (en) 2002-05-17 2002-05-17 A method for in vitro molecular evolution of protein function
GB0211369 2002-05-17
US321195 2002-12-17
US10/321,195 US7153655B2 (en) 1998-06-16 2002-12-17 Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
PCT/GB2003/002102 WO2003097834A2 (en) 2002-05-17 2003-05-16 A method for in vitro molecular evolution of protein function
EP03722867A EP1504098B2 (de) 2002-05-17 2003-05-16 Eine methode zur in vitro molekularen evolution der proteinfunktion

Publications (3)

Publication Number Publication Date
DE60312507D1 true DE60312507D1 (de) 2007-04-26
DE60312507T2 DE60312507T2 (de) 2007-12-06
DE60312507T3 DE60312507T3 (de) 2011-08-18

Family

ID=29551433

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60312507T Expired - Lifetime DE60312507T3 (de) 2002-05-17 2003-05-16 Eine methode zur in vitro molekularen evolution der proteinfunktion

Country Status (10)

Country Link
US (1) US7262012B2 (de)
EP (1) EP1504098B2 (de)
JP (1) JP4372679B2 (de)
AT (1) ATE356871T1 (de)
AU (1) AU2003230027A1 (de)
CA (1) CA2485506C (de)
DE (1) DE60312507T3 (de)
DK (2) DK1504098T4 (de)
ES (1) ES2285118T5 (de)
WO (1) WO2003097834A2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000002913A1 (en) * 1998-07-10 2000-01-20 Jari Pharmaceuticals B.V. Chemotaxis-inhibiting protein of staphylococcus (chips) and its use
EP1118663A1 (de) * 2000-01-07 2001-07-25 Universiteit Utrecht Nukleinsäuren kodierend für Chemotaxis-hemmende Polypeptide
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
EP1586583A3 (de) * 2004-04-16 2005-11-16 Alligator Bioscience AB (publ) Substanzen die der C5a Komplementrezeptor blockieren und deren Verwendung in der Therapie
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
GB0607798D0 (en) 2006-04-20 2006-05-31 Alligator Bioscience Ab Novel polypeptides and use thereof
GB0708376D0 (en) 2007-05-01 2007-06-06 Alligator Bioscience Ab Novel polypeptides and uses thereof
GB0905790D0 (en) 2009-04-03 2009-05-20 Alligator Bioscience Ab Novel polypeptides and use thereof
GB0905503D0 (en) * 2009-03-31 2009-05-13 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
GB0920258D0 (en) 2009-11-19 2010-01-06 Alligator Bioscience Ab New medical agents and use thereof
GB201019086D0 (en) 2010-11-11 2010-12-29 Imp Innovations Ltd Bacterial methods
WO2012122135A2 (en) 2011-03-04 2012-09-13 Evolvemol, Inc. Apparatus and process for isolating specific physical items within a set of physical items
GB201115280D0 (en) 2011-09-05 2011-10-19 Alligator Bioscience Ab Antibodies, uses and methods
GB201311475D0 (en) 2013-06-27 2013-08-14 Alligator Bioscience Ab Polypeptides
CR20170096A (es) 2014-08-12 2017-06-15 Alligator Bioscience Ab Tratamientos conjuntos con anticuerpos anti cd40
KR20180054837A (ko) 2015-09-30 2018-05-24 얀센 바이오테크 인코포레이티드 인간 cd40에 특이적으로 결합하는 작용성 항체 및 사용 방법
CN113892654A (zh) * 2021-10-21 2022-01-07 长春大学 一种以绿豆蛋白粉为原料制备免消化绿豆蛋白质的方法

Family Cites Families (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4888286A (en) 1984-02-06 1989-12-19 Creative Biomolecules, Inc. Production of gene and protein analogs through synthetic gene design using double stranded synthetic oligonucleotides
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DE3588239T3 (de) 1985-03-30 2007-03-08 Kauffman, Stuart A., Santa Fe Verfahren zum Erhalten von DNS, RNS, Peptiden, Polypeptiden oder Proteinen durch DMS-Rekombinant-Verfahren
US6492107B1 (en) * 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5573907A (en) * 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
CA2079802C (en) * 1990-04-05 2001-09-18 Roberto Crea Walk-through mutagenesis
GB9015198D0 (en) * 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US5858725A (en) * 1990-10-10 1999-01-12 Glaxo Wellcome Inc. Preparation of chimaeric antibodies using the recombinant PCR strategy
US5512463A (en) * 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5514568A (en) * 1991-04-26 1996-05-07 Eli Lilly And Company Enzymatic inverse polymerase chain reaction
US5858657A (en) * 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
US5871907A (en) * 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
US5521291A (en) * 1991-09-30 1996-05-28 Boehringer Ingelheim International, Gmbh Conjugates for introducing nucleic acid into higher eucaryotic cells
US5270170A (en) * 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5733731A (en) * 1991-10-16 1998-03-31 Affymax Technologies N.V. Peptide library and screening method
US5252479A (en) * 1991-11-08 1993-10-12 Research Corporation Technologies, Inc. Safe vector for gene therapy
US5733753A (en) * 1992-12-22 1998-03-31 Novo Nordisk A/S Amplification of genomic DNA by site specific integration of a selectable marker construct
US5491074A (en) * 1993-04-01 1996-02-13 Affymax Technologies Nv Association peptides
SE9304060D0 (sv) * 1993-12-06 1993-12-06 Bioinvent Int Ab Sätt att selektera specifika bakteriofager
US6395547B1 (en) * 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6406855B1 (en) * 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6309883B1 (en) * 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5928905A (en) * 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6238884B1 (en) * 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6358709B1 (en) * 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6352842B1 (en) * 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US6171820B1 (en) * 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5939250A (en) * 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US6361974B1 (en) * 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6368798B1 (en) * 1995-12-07 2002-04-09 Diversa Corporation Screening for novel bioactivities
US6096548A (en) * 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US5851804A (en) 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
US5877001A (en) * 1996-06-17 1999-03-02 Diverso Corporation Amidase
US5763239A (en) * 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US5925544A (en) * 1996-11-18 1999-07-20 Novo Nordisk A/S Method of homologous recombination followed by in vivo selection of DNA amplification
WO1998028416A1 (en) 1996-12-20 1998-07-02 Novo Nordisk A/S In vivo recombination
US6326204B1 (en) * 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
EP1007732B1 (de) * 1997-01-17 2006-07-26 Maxygen, Inc. EVOLUTION Prokaryotischer GANZER ZELLEN DURCH REKURSIVE SEQUENZREKOMBINATION
WO1998031816A1 (en) * 1997-01-17 1998-07-23 Regents Of The University Of Minnesota Dna molecules and protein displaying improved triazine compound degrading ability
US6159687A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
US6291165B1 (en) * 1997-03-18 2001-09-18 Novo Nordisk A/S Shuffling of heterologous DNA sequences
US6159688A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods of producing polynucleotide variants
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
GB9712512D0 (en) 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
AU1124499A (en) * 1997-10-28 1999-05-17 Maxygen, Inc. Human papillomavirus vectors
US20010006950A1 (en) * 1998-02-11 2001-07-05 Juha Punnonen Genetic vaccine vector engineering
AU4441699A (en) * 1998-06-17 2000-01-05 Maxygen, Inc. Method for producing polynucleotides with desired properties
US6365408B1 (en) * 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
US20020059659A1 (en) * 1998-08-12 2002-05-16 Maxygen, Inc. DNA shuffling to produce herbicide selective crops
BR9911681A (pt) * 1998-08-12 2001-10-02 Maxygen Inc Embaralhamento de dna para produzir culturas seletivas para herbicidas
WO2000028008A1 (en) * 1998-11-10 2000-05-18 Maxygen, Inc. Modified ribulose 1,5-bisphosphate carboxylase/oxygenase
US6358712B1 (en) * 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
US6368861B1 (en) * 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) * 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6376246B1 (en) * 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
EP1165757A1 (de) * 1999-03-05 2002-01-02 Maxygen, Inc. Erzeugung eines merkmals durch rekombination von split-gen sequenzen
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
JP3777158B2 (ja) * 2000-11-10 2006-05-24 アミコージェン・インコーポレイテッド 一方向性の一本鎖ｄｎａ切片を用いた組換えｄｎａライブラリーの製造方法
CA2430582C (en) * 2000-12-12 2012-12-04 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
US6958213B2 (en) * 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function

Also Published As

Publication number Publication date
CA2485506A1 (en) 2003-11-27
EP1504098B1 (de) 2007-03-14
AU2003230027A1 (en) 2003-12-02
AU2003230027A8 (en) 2003-12-02
ES2285118T3 (es) 2007-11-16
JP2005525820A (ja) 2005-09-02
ATE356871T1 (de) 2007-04-15
US20060166198A1 (en) 2006-07-27
EP1504098B2 (de) 2011-02-09
ES2285118T5 (es) 2012-09-21
DE60312507T3 (de) 2011-08-18
US7262012B2 (en) 2007-08-28
CA2485506C (en) 2012-02-28
DE60312507T2 (de) 2007-12-06
WO2003097834A2 (en) 2003-11-27
DK1504098T4 (da) 2011-05-23
DK1504098T3 (da) 2007-06-11
JP4372679B2 (ja) 2009-11-25
WO2003097834A3 (en) 2004-02-19
EP1504098A2 (de) 2005-02-09
DK200401821A (da) 2004-11-23

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Legal Events

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8363 Opposition against the patent
8366 Restricted maintained after opposition proceedings