DE60222307D1 - Methode zur Anreicherung von Nukleinsäuremolekülen - Google Patents

Methode zur Anreicherung von Nukleinsäuremolekülen

Info

Publication number
DE60222307D1
DE60222307D1 DE60222307T DE60222307T DE60222307D1 DE 60222307 D1 DE60222307 D1 DE 60222307D1 DE 60222307 T DE60222307 T DE 60222307T DE 60222307 T DE60222307 T DE 60222307T DE 60222307 D1 DE60222307 D1 DE 60222307D1
Authority
DE
Germany
Prior art keywords
nucleic acid
microcapsules
enrichment
acid molecules
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60222307T
Other languages
English (en)
Other versions
DE60222307T2 (de
Inventor
Philipp Holliger
Farid Ghadessy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Research Council filed Critical Medical Research Council
Publication of DE60222307D1 publication Critical patent/DE60222307D1/de
Application granted granted Critical
Publication of DE60222307T2 publication Critical patent/DE60222307T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/24—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/44—Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/167—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction with an outer layer or coating comprising drug; with chemically bound drugs or non-active substances on their surface
    • A61K9/1676—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction with an outer layer or coating comprising drug; with chemically bound drugs or non-active substances on their surface having a drug-free core with discrete complete coating layer containing drug
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
DE60222307T 2001-11-16 2002-11-18 Methode zur Anreicherung von Nukleinsäuremolekülen Expired - Lifetime DE60222307T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0127564 2001-11-16
GBGB0127564.3A GB0127564D0 (en) 2001-11-16 2001-11-16 Emulsion compositions

Publications (2)

Publication Number Publication Date
DE60222307D1 true DE60222307D1 (de) 2007-10-18
DE60222307T2 DE60222307T2 (de) 2008-05-29

Family

ID=9925928

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60228607T Expired - Lifetime DE60228607D1 (de) 2001-11-16 2002-11-18 Emulsionshaltige zusammensetzngen
DE60222307T Expired - Lifetime DE60222307T2 (de) 2001-11-16 2002-11-18 Methode zur Anreicherung von Nukleinsäuremolekülen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE60228607T Expired - Lifetime DE60228607D1 (de) 2001-11-16 2002-11-18 Emulsionshaltige zusammensetzngen

Country Status (11)

Country Link
US (6) US7429467B2 (de)
EP (2) EP1518937B1 (de)
JP (2) JP4503290B2 (de)
AT (2) ATE372391T1 (de)
AU (1) AU2002339193B2 (de)
CA (1) CA2467215C (de)
DE (2) DE60228607D1 (de)
DK (1) DK1446504T3 (de)
ES (2) ES2312630T3 (de)
GB (1) GB0127564D0 (de)
WO (1) WO2003044187A2 (de)

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US20100298552A1 (en) 2010-11-25
CA2467215C (en) 2012-10-02
US20050064460A1 (en) 2005-03-24
AU2002339193A1 (en) 2003-06-10
JP2005509436A (ja) 2005-04-14
US20100159592A1 (en) 2010-06-24
EP1446504A2 (de) 2004-08-18
ES2312630T3 (es) 2009-03-01
US7622280B2 (en) 2009-11-24
US20120184011A1 (en) 2012-07-19
AU2002339193B2 (en) 2008-01-31
US20080187968A1 (en) 2008-08-07
GB0127564D0 (en) 2002-01-09
EP1518937B1 (de) 2007-09-05
DK1446504T3 (da) 2008-12-15
JP2007181468A (ja) 2007-07-19
JP4323528B2 (ja) 2009-09-02
WO2003044187A2 (en) 2003-05-30
EP1518937A8 (de) 2005-07-06
CA2467215A1 (en) 2003-05-30
EP1518937A1 (de) 2005-03-30
US20040253731A1 (en) 2004-12-16
US8153402B2 (en) 2012-04-10
WO2003044187A3 (en) 2003-09-04
ES2293162T3 (es) 2008-03-16
EP1446504B1 (de) 2008-08-27
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US7429467B2 (en) 2008-09-30
US7655435B2 (en) 2010-02-02
ATE406460T1 (de) 2008-09-15
ATE372391T1 (de) 2007-09-15
JP4503290B2 (ja) 2010-07-14
US8153364B2 (en) 2012-04-10

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