DE60141087D1 - Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren - Google Patents
Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäurenInfo
- Publication number
- DE60141087D1 DE60141087D1 DE60141087T DE60141087T DE60141087D1 DE 60141087 D1 DE60141087 D1 DE 60141087D1 DE 60141087 T DE60141087 T DE 60141087T DE 60141087 T DE60141087 T DE 60141087T DE 60141087 D1 DE60141087 D1 DE 60141087D1
- Authority
- DE
- Germany
- Prior art keywords
- amplification
- methods
- transcription
- module
- rna transcripts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US21390800P | 2000-06-26 | 2000-06-26 | |
US27774801P | 2001-03-21 | 2001-03-21 | |
PCT/US2001/020660 WO2002000938A2 (en) | 2000-06-26 | 2001-06-26 | Methods and compositions for transcription-based nucleic acid amplification |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60141087D1 true DE60141087D1 (de) | 2010-03-04 |
Family
ID=26908503
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60141087T Expired - Lifetime DE60141087D1 (de) | 2000-06-26 | 2001-06-26 | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
Country Status (10)
Country | Link |
---|---|
US (3) | US6686156B2 (de) |
EP (1) | EP1356094B1 (de) |
JP (1) | JP2004513617A (de) |
CN (1) | CN1498274A (de) |
AT (1) | ATE455186T1 (de) |
AU (1) | AU2001271595A1 (de) |
CA (1) | CA2412721A1 (de) |
DE (1) | DE60141087D1 (de) |
MX (1) | MXPA02012735A (de) |
WO (1) | WO2002000938A2 (de) |
Families Citing this family (64)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
CA2412721A1 (en) * | 2000-06-26 | 2002-01-03 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
AU2002228974A1 (en) * | 2000-12-13 | 2002-06-24 | Nugen Technologies, Inc | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
WO2002064833A1 (fr) | 2001-02-15 | 2002-08-22 | Takara Bio Inc. | Procede de detection de polymorphisme de nucleotide |
EP1366197B1 (de) | 2001-03-09 | 2007-05-09 | Nugen Technologies, Inc. | Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen |
CA2439074A1 (en) * | 2001-03-09 | 2002-09-19 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
US8137911B2 (en) * | 2001-05-22 | 2012-03-20 | Cellscript, Inc. | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
ATE405676T1 (de) * | 2002-03-11 | 2008-09-15 | Nugen Technologies Inc | Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination |
US20030219729A1 (en) * | 2002-03-15 | 2003-11-27 | Tosoh Corporation | Unary avian myeloblastosis virus revers transcriptase and its use |
US20040005614A1 (en) * | 2002-05-17 | 2004-01-08 | Nurith Kurn | Methods for fragmentation, labeling and immobilization of nucleic acids |
JP3923917B2 (ja) * | 2003-03-26 | 2007-06-06 | 株式会社東芝 | ターゲットの製造方法、標的配列検出方法、ターゲットおよび標的配列検出用アッセイキット |
WO2004092418A2 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
WO2005065321A2 (en) * | 2003-12-29 | 2005-07-21 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
US7713697B2 (en) * | 2004-08-27 | 2010-05-11 | Gen-Probe Incorporated | Methods and kits for amplifying DNA |
EP1786916B1 (de) * | 2004-08-27 | 2013-05-08 | Gen-Probe Incorporated | Ein-primer-nukleinsäureamplifikationsverfahren |
KR100695134B1 (ko) * | 2004-11-25 | 2007-03-14 | 삼성전자주식회사 | 층류를 이용한 마이크로어레이 및 이의 제조 방법 |
DK1836302T3 (da) * | 2004-12-23 | 2010-08-02 | Ge Healthcare Bio Sciences | Ligeringsbaseret RNA-amplifikation |
EP1841879A4 (de) * | 2005-01-25 | 2009-05-27 | Population Genetics Technologi | Isothermische dna-amplifikation |
US20070020639A1 (en) * | 2005-07-20 | 2007-01-25 | Affymetrix, Inc. | Isothermal locus specific amplification |
CA2621267A1 (en) | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
US7368265B2 (en) * | 2006-01-23 | 2008-05-06 | Compass Genetics, Llc | Selective genome amplification |
EP2002020A4 (de) * | 2006-04-12 | 2010-07-14 | Siemens Healthcare Diagnostics | Erkennung von einzelnukleotid-polymorphismen aus unverstärkter genomischer dna |
US20090275486A1 (en) * | 2006-05-16 | 2009-11-05 | Nurith Kurn | Nucleic acid separation and purification method based on reversible charge interactions |
US7833716B2 (en) | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
CA2656315A1 (en) * | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
US7501254B2 (en) * | 2006-07-20 | 2009-03-10 | Ghc Technologies, Inc. | Methods and compositions for amplification and capture of nucleic acid sequences |
EP3121286B1 (de) | 2006-12-21 | 2019-11-20 | Gen-Probe Incorporated | Verfahren und zusammensetzungen zur erweiterung von nukleinsäuren |
US8183359B2 (en) * | 2007-03-01 | 2012-05-22 | Gen-Probe Incorporated | Kits for amplifying DNA |
US7794985B2 (en) * | 2007-04-04 | 2010-09-14 | Ghc Technologies, Inc. | Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins |
US9388457B2 (en) * | 2007-09-14 | 2016-07-12 | Affymetrix, Inc. | Locus specific amplification using array probes |
US8716190B2 (en) | 2007-09-14 | 2014-05-06 | Affymetrix, Inc. | Amplification and analysis of selected targets on solid supports |
PL2215265T3 (pl) | 2007-11-14 | 2014-04-30 | Grifols Therapeutics Inc | Sposób opracowania wyników urządzenia do łańcuchowej reakcji polimeryzacji (PCR) |
US8034568B2 (en) * | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
GB2470672B (en) | 2008-03-21 | 2012-09-12 | Nugen Technologies Inc | Methods of RNA amplification in the presence of DNA |
WO2010126913A1 (en) | 2009-04-27 | 2010-11-04 | Gen-Probe Incorporated | Methods and kits for use in the selective amplification of target sequences |
US8512955B2 (en) | 2009-07-01 | 2013-08-20 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
WO2011032053A1 (en) * | 2009-09-11 | 2011-03-17 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
GB2487341A (en) * | 2009-11-02 | 2012-07-18 | Nugen Technologies Inc | Compositions and methods for targeted nucleic acid sequence selection and amplification |
CN102725424B (zh) * | 2010-01-25 | 2014-07-09 | Rd生物科技公司 | 靶核酸的自折叠扩增 |
SG10201510189WA (en) | 2011-10-19 | 2016-01-28 | Nugen Technologies Inc | Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing |
EP2798089B1 (de) | 2011-12-30 | 2018-05-23 | Bio-rad Laboratories, Inc. | Verfahren und zusammensetzungen zur durchführung von nucleinsäureamplifikationsreaktionen |
SG11201404243WA (en) | 2012-01-26 | 2014-08-28 | Nugen Technologies Inc | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
US9487828B2 (en) | 2012-05-10 | 2016-11-08 | The General Hospital Corporation | Methods for determining a nucleotide sequence contiguous to a known target nucleotide sequence |
CA2877094A1 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
US9777319B2 (en) | 2012-06-29 | 2017-10-03 | General Electric Company | Method for isothermal DNA amplification starting from an RNA template |
US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
ES2761920T3 (es) * | 2012-08-30 | 2020-05-21 | Gen Probe Inc | Amplificación de ácido nucleico multifásica |
US9725774B2 (en) | 2012-10-04 | 2017-08-08 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and reagents for detection, quantitation, and serotyping of dengue viruses |
US9410173B2 (en) | 2012-10-24 | 2016-08-09 | Clontech Laboratories, Inc. | Template switch-based methods for producing a product nucleic acid |
US9822408B2 (en) | 2013-03-15 | 2017-11-21 | Nugen Technologies, Inc. | Sequential sequencing |
CA2923812C (en) | 2013-10-17 | 2023-10-17 | Clontech Laboratories, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
EP3068883B1 (de) | 2013-11-13 | 2020-04-29 | Nugen Technologies, Inc. | Zusammensetzungen und verfahren zur identifizierung einer duplikatsequenzierungslesung |
WO2015094861A1 (en) | 2013-12-17 | 2015-06-25 | Clontech Laboratories, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
US10450597B2 (en) | 2014-01-27 | 2019-10-22 | The General Hospital Corporation | Methods of preparing nucleic acids for sequencing |
WO2015131107A1 (en) | 2014-02-28 | 2015-09-03 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
EP3177740B1 (de) | 2014-08-06 | 2021-01-13 | Nugen Technologies, Inc. | Digitale messungen von gezielter sequenzierung |
AU2017328950B2 (en) | 2016-09-15 | 2023-09-14 | Archerdx, Llc | Methods of nucleic acid sample preparation |
WO2018053365A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation for analysis of cell-free dna |
AU2017371324A1 (en) * | 2016-12-09 | 2019-07-11 | Massachusetts Institute Of Technology | CRISPR effector system based diagnostics |
CN106529206B (zh) * | 2016-12-20 | 2019-02-22 | 大连海事大学 | 一种蛋白质二维结构图功能元件的自动布线方法 |
JP2020513815A (ja) * | 2017-03-15 | 2020-05-21 | ザ・ブロード・インスティテュート・インコーポレイテッド | クラスター化短鎖反復回文配列エフェクター系に基づくウイルス検出用診断法 |
US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
CN113528624A (zh) * | 2020-04-17 | 2021-10-22 | 青岛大学 | 扩增和检测核酸的方法及试剂盒 |
Family Cites Families (107)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1982001011A1 (en) | 1980-09-24 | 1982-04-01 | Corp Cetus | Diagnostic method and probe |
US4582788A (en) | 1982-01-22 | 1986-04-15 | Cetus Corporation | HLA typing method and cDNA probes used therein |
ATE53862T1 (de) | 1982-01-22 | 1990-06-15 | Cetus Corp | Verfahren zur charakterisierung von hla und die darin benutzten cdns-testmittel. |
US4786600A (en) | 1984-05-25 | 1988-11-22 | The Trustees Of Columbia University In The City Of New York | Autocatalytic replication of recombinant RNA |
US4683194A (en) | 1984-05-29 | 1987-07-28 | Cetus Corporation | Method for detection of polymorphic restriction sites and nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5011769A (en) | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
US4876187A (en) | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
CA1284931C (en) | 1986-03-13 | 1991-06-18 | Henry A. Erlich | Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids |
CA1338457C (en) | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
DE3634356A1 (de) | 1986-10-08 | 1988-04-21 | Epis Sa | Arzneimittel enthaltend alpha-halogenierte dicarbonsaeuren |
IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
JP2774121B2 (ja) | 1987-07-31 | 1998-07-09 | ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ | 標的ポリヌクレオチド配列の選択的増幅 |
US6090591A (en) | 1987-07-31 | 2000-07-18 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
US6004745A (en) | 1987-09-21 | 1999-12-21 | Gen-Probe Incorporated | Hybridization protection assay |
US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
JP2650159B2 (ja) | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | 核酸増幅方法 |
CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
JP2856804B2 (ja) | 1988-03-24 | 1999-02-10 | ユニバーシティ オブ アイオワ リサーチ ファウンデイション | 標識された相補的核酸プローブを開裂する触媒反応の補因子としての活性に基づく核酸配列検出のための触媒ハイブリッド形成系 |
US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
US5185243A (en) | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
US5508178A (en) | 1989-01-19 | 1996-04-16 | Rose; Samuel | Nucleic acid amplification using single primer |
US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
US6040138A (en) * | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5766849A (en) | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
WO1991001384A1 (en) | 1989-07-11 | 1991-02-07 | Gen-Probe, Incorporated | Nucleic acid sequence amplification methods utilizing a transcription complex |
CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
US5545522A (en) | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
US6013431A (en) | 1990-02-16 | 2000-01-11 | Molecular Tool, Inc. | Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators |
CA2037349C (en) | 1990-03-26 | 2008-06-17 | James G. Wetmur | Branch migration of nucleotides |
HU218095B (hu) | 1990-05-01 | 2000-05-28 | Amgen Inc. | Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban |
US5427911A (en) | 1990-05-01 | 1995-06-27 | Yale University | Coupled amplification and sequencing of DNA |
US5194370A (en) | 1990-05-16 | 1993-03-16 | Life Technologies, Inc. | Promoter ligation activated transcription amplification of nucleic acid sequences |
US5595891A (en) | 1990-07-19 | 1997-01-21 | Behringwerke Ag | Method for producing a polynucleotide for use in single primer amplification |
US5846710A (en) | 1990-11-02 | 1998-12-08 | St. Louis University | Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
AU8997991A (en) | 1991-01-31 | 1992-08-06 | Becton Dickinson & Company | Exonuclease mediated strand displacement amplification |
US6004744A (en) | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
US5888819A (en) | 1991-03-05 | 1999-03-30 | Molecular Tool, Inc. | Method for determining nucleotide identity through primer extension |
US5169766A (en) * | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
ATE231920T1 (de) | 1992-03-11 | 2003-02-15 | Dana Farber Cancer Inst Inc | Methode um mrna zu klonieren |
JP4137996B2 (ja) | 1992-05-06 | 2008-08-20 | ジェン−プローブ・インコーポレイテッド | 核酸配列増幅方法,組成物及びキット |
US5710028A (en) | 1992-07-02 | 1998-01-20 | Eyal; Nurit | Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor |
WO1994003637A1 (en) | 1992-07-31 | 1994-02-17 | Syntex (Usa) Inc. | Method for introducing defined sequences at the 3' end of polynucleotides |
ZA936016B (en) | 1992-08-24 | 1994-03-10 | Akzo Nv | Method for nucleic acid amplification |
US5591575A (en) | 1993-04-07 | 1997-01-07 | Amersham International Plc | Subtraction hybridization employing aziridinylbenoquinone cross-linking agents |
US5837832A (en) * | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
JPH0723799A (ja) | 1993-07-13 | 1995-01-27 | Hitachi Ltd | ポリヌクレオチドの検出方法 |
US6027923A (en) | 1993-07-23 | 2000-02-22 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
FR2708288B1 (fr) | 1993-07-26 | 1995-09-01 | Bio Merieux | Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé. |
WO1995005480A2 (en) | 1993-08-18 | 1995-02-23 | Id Biomedical Corporation | Compositions and methods for detecting target nucleic acid sequences utilizing flanking sequence enzyme molecules |
AU8102694A (en) | 1993-11-17 | 1995-06-06 | Id Biomedical Corporation | Cycling probe cleavage detection of nucleic acid sequences |
CA2140081C (en) | 1994-01-13 | 2008-04-01 | Dean L. Engelhardt | Process, construct and conjugate for producing multiple nucleic acid copies |
US5578832A (en) | 1994-09-02 | 1996-11-26 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
US6060288A (en) * | 1994-08-03 | 2000-05-09 | Mosaic Technologies | Method for performing amplification of nucleic acid on supports |
FR2724934B1 (fr) | 1994-09-26 | 1997-01-24 | Bio Merieux | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
US5556752A (en) | 1994-10-24 | 1996-09-17 | Affymetrix, Inc. | Surface-bound, unimolecular, double-stranded DNA |
US5665545A (en) | 1994-11-28 | 1997-09-09 | Akzo Nobel N.V. | Terminal repeat amplification method |
US5556771A (en) * | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
US5830655A (en) * | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
US5882867A (en) | 1995-06-07 | 1999-03-16 | Dade Behring Marburg Gmbh | Detection of nucleic acids by formation of template-dependent product |
US5763178A (en) | 1995-06-07 | 1998-06-09 | Trevigen, Inc. | Oscillating signal amplifier for nucleic acid detection |
FR2737223B1 (fr) * | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
US5871697A (en) | 1995-10-24 | 1999-02-16 | Curagen Corporation | Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing |
ATE199572T1 (de) | 1995-11-21 | 2001-03-15 | Univ Yale | Unimolekulare segmentamplifikation und bestimmung |
US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
EP0866868B1 (de) | 1995-12-15 | 2001-07-11 | Amersham Life Science Inc | Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität |
US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
GB9604267D0 (en) | 1996-02-29 | 1996-05-01 | Royal Infirmary Of Edinburgh N | Mutation assay |
EP0912761A4 (de) | 1996-05-29 | 2004-06-09 | Cornell Res Foundation Inc | Bestimmung von unterschieden in der nukleisäuresequenz mittels einer kombination von ligasebestimmung und polymerase-kettenreaktion |
US6218105B1 (en) * | 1996-07-19 | 2001-04-17 | Kathleen S. Hall | High throughput papilloma virus in vitro infectivity assay |
EP2264045B1 (de) | 1996-08-14 | 2015-10-21 | Life Technologies Corporation | Stabile Zusammensetzungen zur Amplifizierung und Sequenzierung von Nukleinsäuren |
DE19653439A1 (de) | 1996-12-20 | 1998-07-02 | Svante Dr Paeaebo | Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung |
ATE347615T1 (de) * | 1997-04-16 | 2006-12-15 | Applera Corp | Nukleinsäuresammlung |
US6140086A (en) * | 1997-08-15 | 2000-10-31 | Fox; Donna K. | Methods and compositions for cloning nucleic acid molecules |
JP4317953B2 (ja) * | 1998-01-22 | 2009-08-19 | 独立行政法人理化学研究所 | Dnaの塩基配列決定方法 |
US20010000077A1 (en) | 1998-02-03 | 2001-03-29 | Engelhardt Dean L. | Novel process, construct and conjugate for producing multiple nucleic acid copies |
US6743605B1 (en) | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
US6358712B1 (en) | 1999-01-05 | 2002-03-19 | Trustee Of Boston University | Ordered gene assembly |
US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
WO2000070095A2 (en) * | 1999-05-17 | 2000-11-23 | Dade Behring Inc. | Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide |
US6132997A (en) | 1999-05-28 | 2000-10-17 | Agilent Technologies | Method for linear mRNA amplification |
JP3929775B2 (ja) * | 1999-09-13 | 2007-06-13 | ニューゲン テクノロジーズ, インコーポレイテッド | ポリヌクレオチド配列の線形等温増幅のための方法および組成物 |
US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6300073B1 (en) * | 1999-10-01 | 2001-10-09 | Clontech Laboratories, Inc. | One step RT-PCR methods, enzyme mixes and kits for use in practicing the same |
AU2001251069A1 (en) * | 2000-03-28 | 2001-10-08 | The Government of the United States of America, as represented by the Secretary, Department of Health and Human Services, The NationalInstitutes Of Health | Gene profiling arrays |
CA2412721A1 (en) | 2000-06-26 | 2002-01-03 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US6815164B2 (en) | 2000-10-06 | 2004-11-09 | Nugen Technologies, Inc. | Methods and probes for detection and/or quantification of nucleic acid sequences |
US6673549B1 (en) * | 2000-10-12 | 2004-01-06 | Incyte Corporation | Genes expressed in C3A liver cell cultures treated with steroids |
AR031640A1 (es) * | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
EP1366197B1 (de) | 2001-03-09 | 2007-05-09 | Nugen Technologies, Inc. | Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen |
CA2439074A1 (en) | 2001-03-09 | 2002-09-19 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
EP1420069A4 (de) * | 2001-08-20 | 2005-11-02 | Takara Bio Inc | Nukleinsäureamplifikationsverfahren |
US6617137B2 (en) * | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
US20030204331A1 (en) * | 2001-11-20 | 2003-10-30 | Duncan Whitney | Automated sample preparation methods and devices |
CA2477670A1 (en) * | 2002-03-15 | 2003-09-25 | Arcturus Bioscience, Inc. | Improved nucleic acid amplification |
ES2380893T3 (es) * | 2003-01-29 | 2012-05-21 | 454 Life Sciences Corporation | Amplificación de ácidos nucleicos en emulsión en perlas |
US20040241732A1 (en) * | 2003-04-11 | 2004-12-02 | Chu-An Chang | Method of generating long nucleic acid molecules of defined sequence |
WO2005065321A2 (en) * | 2003-12-29 | 2005-07-21 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
CA2621146A1 (en) * | 2005-09-06 | 2007-03-15 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
US20090275486A1 (en) * | 2006-05-16 | 2009-11-05 | Nurith Kurn | Nucleic acid separation and purification method based on reversible charge interactions |
CA2656315A1 (en) * | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
-
2001
- 2001-06-26 CA CA002412721A patent/CA2412721A1/en not_active Abandoned
- 2001-06-26 US US09/893,191 patent/US6686156B2/en not_active Expired - Fee Related
- 2001-06-26 AT AT01950624T patent/ATE455186T1/de not_active IP Right Cessation
- 2001-06-26 CN CNA018132979A patent/CN1498274A/zh active Pending
- 2001-06-26 MX MXPA02012735A patent/MXPA02012735A/es not_active Application Discontinuation
- 2001-06-26 DE DE60141087T patent/DE60141087D1/de not_active Expired - Lifetime
- 2001-06-26 AU AU2001271595A patent/AU2001271595A1/en not_active Abandoned
- 2001-06-26 JP JP2002506251A patent/JP2004513617A/ja not_active Withdrawn
- 2001-06-26 EP EP01950624A patent/EP1356094B1/de not_active Expired - Lifetime
- 2001-06-26 WO PCT/US2001/020660 patent/WO2002000938A2/en active Application Filing
-
2003
- 2003-10-14 US US10/686,466 patent/US7294461B2/en not_active Expired - Fee Related
-
2007
- 2007-10-31 US US11/933,332 patent/US20080182300A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20020058270A1 (en) | 2002-05-16 |
WO2002000938A9 (en) | 2003-07-03 |
CN1498274A (zh) | 2004-05-19 |
EP1356094A2 (de) | 2003-10-29 |
US20080182300A1 (en) | 2008-07-31 |
US20040203019A1 (en) | 2004-10-14 |
US7294461B2 (en) | 2007-11-13 |
JP2004513617A (ja) | 2004-05-13 |
CA2412721A1 (en) | 2002-01-03 |
MXPA02012735A (es) | 2004-04-20 |
EP1356094B1 (de) | 2010-01-13 |
AU2001271595A1 (en) | 2002-01-08 |
US6686156B2 (en) | 2004-02-03 |
ATE455186T1 (de) | 2010-01-15 |
WO2002000938A2 (en) | 2002-01-03 |
WO2002000938A3 (en) | 2003-08-28 |
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