DE60026729D1 - Verfahren zur modifikation von ausgewählten zellen in einer magnetischen zelltrennungsäule - Google Patents

Verfahren zur modifikation von ausgewählten zellen in einer magnetischen zelltrennungsäule

Info

Publication number
DE60026729D1
DE60026729D1 DE60026729T DE60026729T DE60026729D1 DE 60026729 D1 DE60026729 D1 DE 60026729D1 DE 60026729 T DE60026729 T DE 60026729T DE 60026729 T DE60026729 T DE 60026729T DE 60026729 D1 DE60026729 D1 DE 60026729D1
Authority
DE
Germany
Prior art keywords
selected cells
cell separation
separation column
magnetic cell
modifying selected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60026729T
Other languages
English (en)
Other versions
DE60026729T2 (de
Inventor
Stefan Miltenyi
Mario Assenmacher
Juergen Schmitz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Miltenyi Biotec GmbH
Original Assignee
Miltenyi Biotec GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miltenyi Biotec GmbH filed Critical Miltenyi Biotec GmbH
Publication of DE60026729D1 publication Critical patent/DE60026729D1/de
Application granted granted Critical
Publication of DE60026729T2 publication Critical patent/DE60026729T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/002—High gradient magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/025—High gradient magnetic separators
    • B03C1/031—Component parts; Auxiliary operations
    • B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
    • B03C1/034—Component parts; Auxiliary operations characterised by the magnetic circuit characterised by the matrix elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/806—Electrical property or magnetic property
DE60026729T 1999-09-03 2000-09-01 Verfahren zur modifikation von ausgewählten zellen in einer magnetischen zelltrennungsäule Expired - Lifetime DE60026729T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15240899P 1999-09-03 1999-09-03
US152408P 1999-09-03
PCT/IB2000/001739 WO2001017687A2 (en) 1999-09-03 2000-09-01 Methods of modification of selected cells in a magnetic cell separation column

Publications (2)

Publication Number Publication Date
DE60026729D1 true DE60026729D1 (de) 2006-05-11
DE60026729T2 DE60026729T2 (de) 2006-12-14

Family

ID=22542799

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60026729T Expired - Lifetime DE60026729T2 (de) 1999-09-03 2000-09-01 Verfahren zur modifikation von ausgewählten zellen in einer magnetischen zelltrennungsäule

Country Status (9)

Country Link
US (1) US6468432B1 (de)
EP (1) EP1207961B1 (de)
JP (1) JP4612982B2 (de)
AT (1) ATE320314T1 (de)
AU (1) AU778569B2 (de)
CA (1) CA2383791C (de)
DE (1) DE60026729T2 (de)
ES (1) ES2260063T3 (de)
WO (1) WO2001017687A2 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6649419B1 (en) * 2000-11-28 2003-11-18 Large Scale Proteomics Corp. Method and apparatus for protein manipulation
JP2005502727A (ja) * 2001-09-21 2005-01-27 カプリオン ファーマシューティカルズ インコーポレーティッド 高度に精製された原形質膜の調製方法
US20030099954A1 (en) * 2001-11-26 2003-05-29 Stefan Miltenyi Apparatus and method for modification of magnetically immobilized biomolecules
US20040142384A1 (en) * 2003-01-16 2004-07-22 Cohen Barb Ariel Magnetic separator
DE10362104B4 (de) * 2003-08-29 2008-02-14 Kist-Europe Forschungsgesellschaft Mbh Zellprozessor zur Krankheitsbehandlung
WO2005065267A2 (en) * 2003-12-24 2005-07-21 Massachusetts Institute Of Technology Magnetophoretic cell clarification
GB2425498A (en) * 2005-04-25 2006-11-01 Dynal Biotech Asa A magnetic separation device
US20090208981A1 (en) * 2005-05-03 2009-08-20 Jia-Ming Chang Method for Analysis of Interaction Between Small Molecules and Cells and Apparatus thereof
US20090081750A1 (en) * 2007-02-14 2009-03-26 Bio-Rad Laboratories, Inc. Transfection in magnetically driven continuous flow
EP2229441B1 (de) * 2007-12-12 2014-10-01 The Board of Trustees of The Leland Stanford Junior University Verfahren und vorrichtung zur magnetischen trennung von zellen
KR101384142B1 (ko) * 2007-12-28 2014-04-14 삼성디스플레이 주식회사 표시기판, 이의 제조방법 및 이를 갖는 표시장치
US8790916B2 (en) 2009-05-14 2014-07-29 Genestream, Inc. Microfluidic method and system for isolating particles from biological fluid
JP5678565B2 (ja) 2009-12-08 2015-03-04 Jnc株式会社 磁性微粒子およびその製造方法
DE102010042723A1 (de) * 2010-10-20 2012-04-26 Miltenyi Biotec Gmbh Vorrichtung und Verfahren zur Separation von Neél- und Brown-magnetischen Partikeln
US8695618B2 (en) 2010-12-22 2014-04-15 Carnegie Mellon University 3D chemical pattern control in 2D fluidics devices
EP2518504A1 (de) * 2011-04-29 2012-10-31 Miltenyi Biotec GmbH Verfahren zur quantitativen und qualitativen Charakterisierung von antigenspezifischen T-Zellen, die ein spezifischen Antigen erkennen
LT2734307T (lt) * 2011-07-21 2020-07-10 Menarini Silicon Biosystems S.P.A. Kraujyje cirkuliuojančių daugybinės mielomos ląstelių fiksavimo ir nustatymo testas
EP3037170A1 (de) * 2014-12-27 2016-06-29 Miltenyi Biotec GmbH Multisort-Zelltrennverfahren
EP3247808B1 (de) 2015-01-21 2021-05-05 Fred Hutchinson Cancer Research Center Poc- und/oder tragbare plattform für gentherapie
CN109459571A (zh) * 2018-11-08 2019-03-12 浙江大学 基于微流控和化学发光免疫分析的超顺磁纳米微粒外泌体分离检测系统及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4746590A (en) 1988-12-28 1990-08-01 Stefan Miltenyi Methods and materials for high gradient magnetic separation of biological materials
US5200084A (en) 1990-09-26 1993-04-06 Immunicon Corporation Apparatus and methods for magnetic separation
US5622831A (en) 1990-09-26 1997-04-22 Immunivest Corporation Methods and devices for manipulation of magnetically collected material
WO1994011078A1 (en) 1992-11-16 1994-05-26 Immunivest Corporation Magnetic immobilization and manipulation of biological entities
AU679949B2 (en) * 1992-10-21 1997-07-17 Stefan Miltenyi Direct selection of cells by secretion product
US5514340A (en) * 1994-01-24 1996-05-07 Magnetix Biotechnology, Inc. Device for separating magnetically labelled cells
GB9413029D0 (en) 1994-06-29 1994-08-17 Common Services Agency Stem cell immobilisation
US5705059A (en) * 1995-02-27 1998-01-06 Miltenyi; Stefan Magnetic separation apparatus
US6190870B1 (en) * 1995-08-28 2001-02-20 Amcell Corporation Efficient enrichment and detection of disseminated tumor cells
GB9523045D0 (en) * 1995-11-10 1996-01-10 Genera Tech Ltd Methods of capture and asssy procedures
WO1998005791A1 (en) * 1996-08-02 1998-02-12 Immunivest Corporation Method to select and transfect cell subpopulations
JPH10179165A (ja) * 1996-12-27 1998-07-07 Norin Suisansyo Chikusan Shikenjo 抗体磁気ビーズを用いた生殖幹細胞の分離、精製と幹細胞株の樹立方法

Also Published As

Publication number Publication date
ES2260063T3 (es) 2006-11-01
EP1207961B1 (de) 2006-03-15
WO2001017687A3 (en) 2002-01-17
JP4612982B2 (ja) 2011-01-12
AU778569B2 (en) 2004-12-09
CA2383791C (en) 2012-11-06
JP2003508073A (ja) 2003-03-04
ATE320314T1 (de) 2006-04-15
WO2001017687A2 (en) 2001-03-15
CA2383791A1 (en) 2001-03-15
AU1294801A (en) 2001-04-10
DE60026729T2 (de) 2006-12-14
EP1207961A2 (de) 2002-05-29
US6468432B1 (en) 2002-10-22

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