DE60014365D1 - Fluoreszenzpolarizationsbestimmungen unter verwendung von polyionen - Google Patents

Fluoreszenzpolarizationsbestimmungen unter verwendung von polyionen

Info

Publication number
DE60014365D1
DE60014365D1 DE60014365T DE60014365T DE60014365D1 DE 60014365 D1 DE60014365 D1 DE 60014365D1 DE 60014365 T DE60014365 T DE 60014365T DE 60014365 T DE60014365 T DE 60014365T DE 60014365 D1 DE60014365 D1 DE 60014365D1
Authority
DE
Germany
Prior art keywords
reagent
mixture
polyions
fluorescence polarization
reagent mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60014365T
Other languages
English (en)
Other versions
DE60014365T2 (de
Inventor
T Nikiforov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caliper Life Sciences Inc
Original Assignee
Caliper Life Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/316,447 external-priority patent/US6287774B1/en
Application filed by Caliper Life Sciences Inc filed Critical Caliper Life Sciences Inc
Application granted granted Critical
Publication of DE60014365D1 publication Critical patent/DE60014365D1/de
Publication of DE60014365T2 publication Critical patent/DE60014365T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
DE60014365T 1999-05-21 2000-05-11 Fluoreszenzpolarizationsbestimmungen unter verwendung von polyionen Expired - Lifetime DE60014365T2 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US09/316,447 US6287774B1 (en) 1999-05-21 1999-05-21 Assay methods and system
US316447 1999-05-21
US13956299P 1999-06-16 1999-06-16
US139562P 1999-06-16
US15636699P 1999-09-28 1999-09-28
US156366P 1999-09-28
PCT/US2000/013293 WO2000072016A1 (en) 1999-05-21 2000-05-11 Fluorescence polarization assays involving polyions

Publications (2)

Publication Number Publication Date
DE60014365D1 true DE60014365D1 (de) 2004-11-04
DE60014365T2 DE60014365T2 (de) 2005-10-13

Family

ID=27385362

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60029363T Expired - Lifetime DE60029363T2 (de) 1999-05-21 2000-05-11 Fluoreszenzverfahren zum Messen von Enzymaktivität unter Verwendung von Polyionen
DE60014365T Expired - Lifetime DE60014365T2 (de) 1999-05-21 2000-05-11 Fluoreszenzpolarizationsbestimmungen unter verwendung von polyionen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE60029363T Expired - Lifetime DE60029363T2 (de) 1999-05-21 2000-05-11 Fluoreszenzverfahren zum Messen von Enzymaktivität unter Verwendung von Polyionen

Country Status (10)

Country Link
EP (1) EP1183536B1 (de)
JP (1) JP4472879B2 (de)
CN (1) CN1130564C (de)
AT (2) ATE332978T1 (de)
AU (1) AU762155B2 (de)
CA (1) CA2373747A1 (de)
DE (2) DE60029363T2 (de)
IL (1) IL146375A0 (de)
MX (1) MXPA01011831A (de)
NZ (1) NZ515384A (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2819747B1 (de) * 2012-02-28 2016-02-17 Koninklijke Philips N.V. Vorrichtung für energetische hautbehandlung
CN104048922A (zh) * 2014-06-26 2014-09-17 北京理工大学 一种荧光光谱偏振度和偏振角的测量方法
CN113091808A (zh) * 2021-03-29 2021-07-09 江苏利宏科技发展有限公司 一种具有综合信息管理系统的化工工控仪表及其系统

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5445935A (en) * 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
WO1998018956A1 (en) * 1996-10-28 1998-05-07 Panvera Corporation Kinase activity measurement using fluorescence polarization

Also Published As

Publication number Publication date
JP4472879B2 (ja) 2010-06-02
JP2003502620A (ja) 2003-01-21
NZ515384A (en) 2003-08-29
ATE332978T1 (de) 2006-08-15
DE60029363T2 (de) 2007-08-02
MXPA01011831A (es) 2003-09-04
AU762155B2 (en) 2003-06-19
EP1183536A1 (de) 2002-03-06
ATE278190T1 (de) 2004-10-15
CN1355884A (zh) 2002-06-26
CA2373747A1 (en) 2000-11-30
DE60014365T2 (de) 2005-10-13
AU5134300A (en) 2000-12-12
DE60029363D1 (de) 2006-08-24
CN1130564C (zh) 2003-12-10
IL146375A0 (en) 2002-07-25
EP1183536B1 (de) 2004-09-29

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