DE3700122A1 - Chamber for separating a particle mixture according to the countercurrent centrifugation method - Google Patents

Chamber for separating a particle mixture according to the countercurrent centrifugation method

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Publication number
DE3700122A1
DE3700122A1 DE19873700122 DE3700122A DE3700122A1 DE 3700122 A1 DE3700122 A1 DE 3700122A1 DE 19873700122 DE19873700122 DE 19873700122 DE 3700122 A DE3700122 A DE 3700122A DE 3700122 A1 DE3700122 A1 DE 3700122A1
Authority
DE
Germany
Prior art keywords
chamber
particles
separating
separation
flow
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19873700122
Other languages
German (de)
Inventor
Manfred Lutz
Wolfgang Dr Rer Nat Hartmann
Gerhard Dr Med Gaedicke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to DE19873700122 priority Critical patent/DE3700122A1/en
Publication of DE3700122A1 publication Critical patent/DE3700122A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/06—Centrifugal counter-current apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B04B2005/0471—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation with additional elutriation separation of different particles

Abstract

In counterstream centrifugation technology, in addition to the centrifugal force, forces of a centripetally directed flow of liquid act on a mixture of particles. An equilibrium of forces is established as a function of the size and density of the particles. By changing the force ratios, particles of different sizes and density can be isolated from the liquid flowing off in centripetal fashion. The geometric profile of the separating area, the so-called separating chamber, is decisive for the selectivity of separation. By virtue of eddy effects of the liquid flow and by virtue of non-uniform distribution of flow, the separation selectivity is unfavourably influenced in previously known separating chambers. In the described separating chamber, a uniform flow behaviour and a uniform distribution of the particles in the separating chamber are achieved by means of a central inlet opening which is aligned in the longitudinal direction of the chamber and by means of a constantly increasing cross-section of the chamber. As a result, the precise, non-damaging separation of large amounts of a mixture of living cells is made possible.

Description

Aus einem Gemisch von Teilchen sollen einzelne Fraktionen in möglichst großer Reinheit gewonnen werden.Individuals are said to consist of a mixture of particles Fractions obtained in the greatest possible purity will.

P. E. Lindahl hat (Nature 161: 648-649; 1948) eine Technik beschrieben, bei der in einem Zentrifugen­ rotor zusätzlich zu der auf Teilchen wirkenden Zentrifugalkraft eine zentripetale Flüssigkeits­ strömung wirkt. Abhängig von Größe und Dichte der Teilchen bildet sich ein Gleichgewicht beider Kräfte. Aus der nach zentral abfließenden Flüs­ sigkeit lassen sich durch Änderung von Rotorge­ schwindigkeit beziehungsweise Flüssigkeitsströmung Fraktionen unterschiedlicher Größe und Dichte ge­ winnen. Maßgeblich für die Schärfe der Trennung ist das geometrische Profil des Trennbereichs, die sogenannte Trennkammer.P.E. Lindahl (Nature 161: 648-649; 1948) has one Technique described in a centrifuge rotor in addition to that acting on particles Centrifugal force is a centripetal fluid flow works. Depending on the size and density of the Particles form an equilibrium between the two Powers. From the centrally flowing rivers liquid can be changed by changing the rotor speed or fluid flow Fractions of different sizes and densities win. Decisive for the sharpness of the separation is the geometric profile of the separation area, the so-called separation chamber.

In Patent 43 50 283 wird von BECKMAN Instru­ ments ein Rotor mit Trennkammer beschrieben.In patent 43 50 283 BECKMAN Instru ment described a rotor with separation chamber.

In dieser Trennkammer treten unregelmäßige Strö­ mungsverteilungen und Verwirbelungen der Flüssig­ keitsströme auf. Dies führt zu ungleichmäßiger Teilchenverteilung, zu unscharfer Trennung und bei lebenden Zellen zu Verklumpung und Zellzerstörung.Irregular flows occur in this separation chamber Distribution of distribution and turbulence of the liquid currents. This leads to more uneven Particle distribution, blurred separation and at living cells for clumping and cell destruction.

Die Erfindung soll durch ein geeignetes Kammerpro­ fil den Strömungsverlauf gleichmäßig gestalten, Verwirbelungen vermindern und die effektive Trenn­ strecke vergrößern. Es wird dadurch eine schonen­ dere und präzisere Trennung ermöglicht. Gleichzei­ tig steigt die maximal mögliche Beladbarkeit mit Teilchen.The invention is intended by a suitable Kammerpro fil make the flow even, Reduce turbulence and the effective separation enlarge distance. It will be gentle on it enables more precise separation. Simultaneously The maximum possible loading capacity also increases Particles.

Die Aufgabe wird erfindungsgemäß dadurch gelöst, daß eine großvolumige, zentrale, in der Längs­ achse ausgerichtete Einlaßöffnung (1) und ein sich konstant vergrößernder Querschnitt (2) sich dergestalt ergänzen, daß ein gleichmäßiger Strö­ mungsverlauf und eine gleichmäßige Verteilung der Teilchen erreicht wird. The object is achieved in that a large-volume, central, in the longitudinal axis aligned inlet opening ( 1 ) and a constantly increasing cross-section ( 2 ) complement each other in such a way that a uniform current flow and a uniform distribution of the particles is achieved.

Die Trennschärfe wird erhöht, die Teilchen schonen­ der getrennt und die maximal mögliche Beladung mit Teilchen deutlich gesteigert.The selectivity is increased, the particles protect the separated and the maximum possible load with Particles increased significantly.

Ein Ausführungsbeispiel der Erfindung ist in Zeichnungen dargestellt. Es zeigtAn embodiment of the invention is in Drawings shown. It shows

Zeichnung 1 eine Aufsicht mit Einlaßöffnung (1),1 shows a top view with an inlet opening ( 1 ),

Zeichnung 2 eine Seitenansicht mit Einlaßöffnung (1).Drawing 2 is a side view with inlet opening ( 1 ).

Claims (1)

Kammer zur Trennung eines Teilchengemisches nach dem Gegenstromzentrifugationsverfahren, gekennzeichnet durch eine großvolumige, zentrale, in der Längsachse ausgerichtete Einlaßöffnung (1) und einen sich konstant vergrößernden Querschnitt (2), dergestalt, daß ein gleichmäßiger Strömungs­ verlauf und eine gleichmäßige Verteilung der Teilchen erreicht wird.Chamber for separating a particle mixture according to the countercurrent centrifugation process, characterized by a large-volume, central inlet opening ( 1 ) aligned in the longitudinal axis and a constantly increasing cross-section ( 2 ) such that a uniform flow and a uniform distribution of the particles is achieved.
DE19873700122 1987-01-03 1987-01-03 Chamber for separating a particle mixture according to the countercurrent centrifugation method Withdrawn DE3700122A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE19873700122 DE3700122A1 (en) 1987-01-03 1987-01-03 Chamber for separating a particle mixture according to the countercurrent centrifugation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19873700122 DE3700122A1 (en) 1987-01-03 1987-01-03 Chamber for separating a particle mixture according to the countercurrent centrifugation method

Publications (1)

Publication Number Publication Date
DE3700122A1 true DE3700122A1 (en) 1988-07-14

Family

ID=6318444

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19873700122 Withdrawn DE3700122A1 (en) 1987-01-03 1987-01-03 Chamber for separating a particle mixture according to the countercurrent centrifugation method

Country Status (1)

Country Link
DE (1) DE3700122A1 (en)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996033023A1 (en) * 1995-04-18 1996-10-24 Cobe Laboratories, Inc. Particle separation apparatus and method
NL1002569C2 (en) * 1996-03-11 1997-09-12 Univ Delft Tech Method for carrying out a treatment in the presence of a centrifugal force and device therefor.
US5674173A (en) * 1995-04-18 1997-10-07 Cobe Laboratories, Inc. Apparatus for separating particles
US5906570A (en) * 1995-04-18 1999-05-25 Cobe Laboratories, Inc. Particle filter apparatus
US6022306A (en) * 1995-04-18 2000-02-08 Cobe Laboratories, Inc. Method and apparatus for collecting hyperconcentrated platelets
US6051146A (en) * 1998-01-20 2000-04-18 Cobe Laboratories, Inc. Methods for separation of particles
US6053856A (en) * 1995-04-18 2000-04-25 Cobe Laboratories Tubing set apparatus and method for separation of fluid components
US6153113A (en) 1999-02-22 2000-11-28 Cobe Laboratories, Inc. Method for using ligands in particle separation
US6334842B1 (en) 1999-03-16 2002-01-01 Gambro, Inc. Centrifugal separation apparatus and method for separating fluid components
US6354986B1 (en) 2000-02-16 2002-03-12 Gambro, Inc. Reverse-flow chamber purging during centrifugal separation
WO2008156906A1 (en) * 2007-06-19 2008-12-24 Caridianbct, Inc. Blood processing apparatus with flared cell capture chamber
US7708889B2 (en) 2002-04-16 2010-05-04 Caridianbct, Inc. Blood component processing system method
US7963901B2 (en) 2008-09-12 2011-06-21 Caridianbct, Inc. Blood processing apparatus with cell capture chamber with protruding inlet
US9248446B2 (en) 2013-02-18 2016-02-02 Terumo Bct, Inc. System for blood separation with a separation chamber having an internal gravity valve
US10765971B2 (en) 2014-08-14 2020-09-08 Terumo Bct, Inc. Three-port chamber for processing particles
US11964286B2 (en) 2019-04-12 2024-04-23 Terumo Bct, Inc. Cell washing chamber for blood processing centrifuge

Cited By (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5913768A (en) * 1995-04-18 1999-06-22 Cobe Laboratories, Inc. Particle filter apparatus
US5674173A (en) * 1995-04-18 1997-10-07 Cobe Laboratories, Inc. Apparatus for separating particles
US5722926A (en) * 1995-04-18 1998-03-03 Cobe Laboratories, Inc. Method for separating particles
US5906570A (en) * 1995-04-18 1999-05-25 Cobe Laboratories, Inc. Particle filter apparatus
US5951877A (en) * 1995-04-18 1999-09-14 Cobe Laboratories, Inc. Particle filter method
US6022306A (en) * 1995-04-18 2000-02-08 Cobe Laboratories, Inc. Method and apparatus for collecting hyperconcentrated platelets
US6053856A (en) * 1995-04-18 2000-04-25 Cobe Laboratories Tubing set apparatus and method for separation of fluid components
US6071422A (en) * 1995-04-18 2000-06-06 Cobe Laboratories, Inc. Particle separation method and apparatus
WO1996033023A1 (en) * 1995-04-18 1996-10-24 Cobe Laboratories, Inc. Particle separation apparatus and method
NL1002569C2 (en) * 1996-03-11 1997-09-12 Univ Delft Tech Method for carrying out a treatment in the presence of a centrifugal force and device therefor.
WO1997033687A1 (en) * 1996-03-11 1997-09-18 Bird Engineering B.V. Method of carrying out a treatment in the presence of a centrifugal force and an apparatus therefor
US6180394B1 (en) 1996-03-11 2001-01-30 Bird Engineering B.V. Method of carrying out a treatment in the presence of a centrifugal force and an apparatus therefor
US6051146A (en) * 1998-01-20 2000-04-18 Cobe Laboratories, Inc. Methods for separation of particles
US6280622B1 (en) 1999-02-22 2001-08-28 Gambro, Inc. System for using ligands in particle separation
US6153113A (en) 1999-02-22 2000-11-28 Cobe Laboratories, Inc. Method for using ligands in particle separation
US6334842B1 (en) 1999-03-16 2002-01-01 Gambro, Inc. Centrifugal separation apparatus and method for separating fluid components
US6514189B1 (en) 1999-03-16 2003-02-04 Gambro, Inc. Centrifugal separation method for separating fluid components
US6354986B1 (en) 2000-02-16 2002-03-12 Gambro, Inc. Reverse-flow chamber purging during centrifugal separation
US7708889B2 (en) 2002-04-16 2010-05-04 Caridianbct, Inc. Blood component processing system method
US7857744B2 (en) 2007-06-19 2010-12-28 Caridianbct, Inc. Blood processing apparatus with flared cell capture chamber and method
WO2008156906A1 (en) * 2007-06-19 2008-12-24 Caridianbct, Inc. Blood processing apparatus with flared cell capture chamber
US7963901B2 (en) 2008-09-12 2011-06-21 Caridianbct, Inc. Blood processing apparatus with cell capture chamber with protruding inlet
US8226537B2 (en) 2008-09-12 2012-07-24 Terumo Bct, Inc. Blood processing apparatus with cell separation chamber with baffles
US9248446B2 (en) 2013-02-18 2016-02-02 Terumo Bct, Inc. System for blood separation with a separation chamber having an internal gravity valve
US10765971B2 (en) 2014-08-14 2020-09-08 Terumo Bct, Inc. Three-port chamber for processing particles
US11376525B2 (en) 2014-08-14 2022-07-05 Terumo Bct, Inc. Three-port chamber for processing particles
US11446589B2 (en) 2014-08-14 2022-09-20 Terumo Bct, Inc. Three-port chamber for processing particles
US11813553B2 (en) 2014-08-14 2023-11-14 Terumo Bct, Inc. Three-port chamber for processing particles
US11964286B2 (en) 2019-04-12 2024-04-23 Terumo Bct, Inc. Cell washing chamber for blood processing centrifuge

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