DE3667291D1 - Schnelle und spezifische immobilisierung von nukleinsaeuren am festen traeger. - Google Patents

Schnelle und spezifische immobilisierung von nukleinsaeuren am festen traeger.

Info

Publication number
DE3667291D1
DE3667291D1 DE8686101883T DE3667291T DE3667291D1 DE 3667291 D1 DE3667291 D1 DE 3667291D1 DE 8686101883 T DE8686101883 T DE 8686101883T DE 3667291 T DE3667291 T DE 3667291T DE 3667291 D1 DE3667291 D1 DE 3667291D1
Authority
DE
Germany
Prior art keywords
nucleoside
phosphate
oligonucleotide
fast
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8686101883T
Other languages
English (en)
Inventor
Nanibhushan Dattagupta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Molecular Diagnostics Inc
Original Assignee
Molecular Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Diagnostics Inc filed Critical Molecular Diagnostics Inc
Application granted granted Critical
Publication of DE3667291D1 publication Critical patent/DE3667291D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
DE8686101883T 1985-02-22 1986-02-14 Schnelle und spezifische immobilisierung von nukleinsaeuren am festen traeger. Expired - Fee Related DE3667291D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/704,129 US4818681A (en) 1985-02-22 1985-02-22 Fast and specific immobilization of nucleic acids to solid supports

Publications (1)

Publication Number Publication Date
DE3667291D1 true DE3667291D1 (de) 1990-01-11

Family

ID=24828201

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686101883T Expired - Fee Related DE3667291D1 (de) 1985-02-22 1986-02-14 Schnelle und spezifische immobilisierung von nukleinsaeuren am festen traeger.

Country Status (5)

Country Link
US (1) US4818681A (de)
EP (1) EP0192197B1 (de)
JP (1) JPS61246201A (de)
AT (1) ATE48431T1 (de)
DE (1) DE3667291D1 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4948882A (en) * 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
JPH084519B2 (ja) * 1987-12-25 1996-01-24 エフ・ホフマン―ラ ロシュ アーゲー ハイブリッド形成用担体およびその調整方法
US5352578A (en) * 1989-02-15 1994-10-04 Worcester Foundation For Experimental Biology Method of separating oligonucleotides from a mixture
US5559221A (en) * 1989-02-15 1996-09-24 Worcester Foundation For Experimental Biology Method of separating oligonucleotides from a mixture
WO1991000868A1 (en) * 1989-07-07 1991-01-24 National Research Development Corporation Immobilised polynucleotides
US5079169A (en) * 1990-05-22 1992-01-07 The Regents Of The Stanford Leland Junior University Method for optically manipulating polymer filaments
DK0604552T3 (da) * 1991-09-18 1997-08-04 Affymax Tech Nv Fremgangsmåde til syntese af forskellige samlinger af oligomerer
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
TW323284B (de) * 1992-03-23 1997-12-21 Novartis Ag
GB9207380D0 (en) * 1992-04-03 1992-05-13 Ici Plc Compounds
US5382512A (en) * 1993-08-23 1995-01-17 Chiron Corporation Assay device with captured particle reagent
US6165778A (en) * 1993-11-02 2000-12-26 Affymax Technologies N.V. Reaction vessel agitation apparatus
US5503805A (en) * 1993-11-02 1996-04-02 Affymax Technologies N.V. Apparatus and method for parallel coupling reactions
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
EP0963443B1 (de) 1996-12-10 2006-03-08 Sequenom, Inc. Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung
US5905024A (en) * 1996-12-17 1999-05-18 University Of Chicago Method for performing site-specific affinity fractionation for use in DNA sequencing
US6048695A (en) * 1998-05-04 2000-04-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
US6979728B2 (en) * 1998-05-04 2005-12-27 Baylor College Of Medicine Articles of manufacture and methods for array based analysis of biological molecules
JP3996307B2 (ja) * 1999-11-05 2007-10-24 富士フイルム株式会社 Ｄｎａ断片の固定方法、ｄｎａチップおよび核酸断片の検出方法
AU2001233043A1 (en) * 2000-01-28 2001-08-07 Linden Technologies, Inc. C-3' protected monomeric nucleotides and synthesis of oligonucleotides on solid support
AU2001255518A1 (en) * 2000-06-07 2001-12-17 Baylor College Of Medicine Compositions and methods for array-based nucleic acid hybridization
US6861214B1 (en) * 2000-10-23 2005-03-01 Beckman Coulter, Inc. Immobilization of biopolymers to aminated substrates by direct adsorption
US20030170675A1 (en) * 2001-04-11 2003-09-11 The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. Methods of manipulating nucleic acids
CA2443894A1 (en) * 2001-04-11 2002-10-24 Charlie Xiang Modified random primers for probe labeling
AU2002367886B8 (en) * 2001-10-12 2008-08-14 Perkinelmer Las, Inc. Compilations of nucleic acids and arrays and methods of using them
US7439346B2 (en) * 2001-10-12 2008-10-21 Perkinelmer Las Inc. Nucleic acids arrays and methods of use therefor
WO2003042697A1 (en) * 2001-11-14 2003-05-22 Genospectra, Inc. Biochemical analysis system with combinatorial chemistry applications
US20030124542A1 (en) * 2001-12-28 2003-07-03 Spectral Genomics, Inc. Methods for mapping the chromosomal loci of genes expressed by a cell
CN1720453A (zh) * 2002-12-02 2006-01-11 东洋钢钣株式会社 将多聚核苷酸固定于固体支持物的方法 ,由此获得的固体固定化支持物 ,从固体支持物上检测目的多聚核苷酸的方法以及用于固定化多聚核苷酸的溶液
WO2014134144A1 (en) 2013-02-28 2014-09-04 The General Hospital Corporation Mirna profiling compositions and methods of use

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1083508A (fr) * 1975-11-13 1980-08-12 Jacques Grange Supports porteurs de chaines laterales, procedes d'obtention de ces supports, procedes de fixation de composes organiques comportant un residu glucidique sur lesdits supports, produits et reactifs resultant de ladite fixation chimique
DE2841414C2 (de) * 1978-09-22 1980-04-03 Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf), 3300 Braunschweig Verfahren zur Herstellung von an Makromoleküle gebundenen, ein Adeninringsystem enthaltenden Koenzymen
JPS5564599A (en) * 1978-11-08 1980-05-15 Unitika Ltd Adenosine triphosphate derivative
AU550148B2 (en) * 1980-02-29 1986-03-06 University Patents Inc. Supports for use in preparation of polynucleotides
US4401796A (en) * 1981-04-30 1983-08-30 City Of Hope Research Institute Solid-phase synthesis of polynucleotides
CA1180647A (en) * 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
FR2519005B1 (fr) * 1981-12-29 1985-10-25 Pasteur Institut Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn
US4472572A (en) * 1982-02-01 1984-09-18 Innovax Laboratories, Ltd. Adenosine pyrophosphate polynucleotide compound
US4474947A (en) * 1982-04-08 1984-10-02 Biosearch Benzazolides and their employment in phosphate ester oligonucleotide synthesis processes
US4474948A (en) * 1982-04-08 1984-10-02 Biosearch Benzazolides and their employment in phosphite ester oligonucleotide synthesis processes
US4591564A (en) * 1982-11-10 1986-05-27 The University Of Montana Transferase enzymes which modify the 3'-termini of ribonucleic acid and methods
WO1984003285A1 (en) * 1983-02-22 1984-08-30 Molecular Biosystems Inc Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis
EP0155950B1 (de) * 1983-09-02 1990-01-17 Molecular Biosystems, Inc. Oligonukleotid-polymer-tragsystem
JPS61501746A (ja) * 1984-04-05 1986-08-21 ライフ　テクノロジ−ズ，インコ−ポレイテツド 核酸の固定化法
FR2567523B1 (fr) * 1984-07-12 1987-11-13 Pasteur Institut Polynucleotides lies de facon covalente a un support solide, et procede pour leur obtention
JPS61112093A (ja) * 1984-11-05 1986-05-30 Wakunaga Seiyaku Kk ヌクレオチド誘導体

Also Published As

Publication number Publication date
JPS61246201A (ja) 1986-11-01
ATE48431T1 (de) 1989-12-15
US4818681A (en) 1989-04-04
EP0192197A3 (en) 1987-12-23
EP0192197B1 (de) 1989-12-06
EP0192197A2 (de) 1986-08-27

Similar Documents

Publication Publication Date Title
DE3667291D1 (de) Schnelle und spezifische immobilisierung von nukleinsaeuren am festen traeger.
ES549428A0 (es) Un procedimiento para la produccion de un acido nucleico inmovilizado y derivados del mismo.
BR8602673A (pt) Processo para recuperacao de quitosano de biomassa contendo quitina
NL194922B (nl) Werkwijze voor de bepaling van nucleïnezuren, alsmede set daarvoor.
BR8703007A (pt) Sistema gerador de plasma e processo para gerar um plasma
PT82219B (pt) Processo para a extraccao e purificacao de proteinas a partir de meios de cultura que as produzem
DE3675684D1 (de) Diagnostisches reagenz, satz und verfahren unter verwendung von polynukleotid-versetzung, trennung, enzymatischer spaltung und nachweis von adenosinphosphat.
BR8702269A (pt) Processo e sistema de geracao de plasma
ATE358177T1 (de) Ein in vitro sortierverfahren
DK0513064T3 (da) Manipulering af faste, halvfaste eller flydende materialer
IT8821481A0 (it) Processo ed apparato per la devolatilizzazione di soluzioni di polimeri.
IT8821076A0 (it) Processo catalitico per laproduzione di ossime.
DE3851400T2 (de) Raffinierungsverfahren von Äthylenglycol.
BR8801487A (pt) Sistema de computador e processo
ES8705920A1 (es) Un metodo de preparacion de un oligonucleotido
BR8504752A (pt) Processo ciclico para a producao de uma solucao alcalina de carbamato de celulose,para a precipitacao do carbamato de celulose da solucao alcalina e para a recuperacao e reciclagem dos produtos quimicos
ATE53588T1 (de) Herstellung von cytosin-nukleosiden.
DE69308391T2 (de) Verfahren zur Trennung von 2-Deoxy-2,2-Difluor-D-Ribofuranosyl-Alkyl-Sulfonat-Anomeren
FR2557894B1 (fr) Procede de traitement de pates papetieres par une solution enzymatique favorisant la fibrillation et pates ainsi traitees.
IT8622449A0 (it) Procedimento di separazione di polvere di polimero e gas disupporto.
IT8721493A0 (it) Processo per la produzione di chetossime.
BR8806087A (pt) Processo,sistema e aparelho para trituracao fina
PT83682A (en) Process for the expression of an aids virus gene
DE3786978T2 (de) Verfahren für die Zuführung von Kulturmedium und Kultursystem.
IT8620655A0 (it) Processo per la sintesi di mono odi idrossifluoroalcani.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee