CN106290842A - Cholera vibrio O 139 colloidal gold method detection kit - Google Patents
Cholera vibrio O 139 colloidal gold method detection kit Download PDFInfo
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- CN106290842A CN106290842A CN201510242775.4A CN201510242775A CN106290842A CN 106290842 A CN106290842 A CN 106290842A CN 201510242775 A CN201510242775 A CN 201510242775A CN 106290842 A CN106290842 A CN 106290842A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/18—Togaviridae; Flaviviridae
- G01N2333/183—Flaviviridae, e.g. pestivirus, mucosal disease virus, bovine viral diarrhoea virus, classical swine fever virus (hog cholera virus) or border disease virus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The present invention relates to field of biological detection, particularly relate to a kind of cholera vibrio O 139 gold-immunochromatographyreagent reagent for assay box and its production and use.The present invention provides a kind of cholera vibrio O 139 gold-immunochromatographyreagent reagent for assay box, including test card, test card includes base plate and is positioned at the sample pad, gold mark pad, nitrocellulose filter and the adsorptive pads that start to be arranged in order from sample-adding end of backplate surface, anti-cholera vibrio O 139 type monoclonal antibody is comprised on described gold mark pad, being coated with detection line and nature controlling line on described nitrocellulose filter, the anti-cholera vibrio O 139 type monoclonal antibody on described gold mark pad uses colloid gold label.Cholera vibrio O 139 gold-immunochromatographyreagent reagent for assay box first passage colloidal gold immunochromatographimethod technology for detection cholera vibrio O 139 provided by the present invention, has susceptiveness and specificity concurrently, has the advantages such as operation is fast and convenient, result is accurate, economic and practical.
Description
Technical field
The present invention relates to field of biological detection, particularly relate to a kind of cholera vibrio O 139 gold-immunochromatographyreagent reagent for assay box and preparation thereof
Method and purposes.
Background technology
Vibrio cholera (Vibrio cholerae) is the pathogen of cholera, can be divided into more than 200 sero-group according to the difference of its O antigen,.
In more than 200 sero-group of vibrio cholera, only Ol group and O139 group cholera vibrio can cause cholera.Cholera is a kind of ancient
Old and popular one of deadly infectious disease widely.The mankind are unique susceptible persons of vibrio cholera.Vibrio cholera enters digestive tract and arrives
Small intestinal, in intestinal mucosal surface absorption and breeds rapidly.The cholera enterotoxin produced acts on mucous membrane of small intestine, causes intestinal fluid excessive
Secretion.Cause the most in the world and be repeatedly very popular, mainly show as vomiting and dirarrhea, cause dehydration and metabolic acidosis, follow
Ring exhaustion, mortality rate is the highest.Belong to international quarantine infectious disease.
Cholera is the category A infectious disease that China is legal, and national requirements quotes assay in the 24h that sees and treat patients.Therefore, identify early
Go out vibrio cholera and the popular and treatment controlling this disease is had extremely important meaning.The fast diagnosis method of vibrio cholera is as fast at present
The speed effect such as immobilization test and immunofluorescence fungus ball test is undesirable, few when being primarily due to many during the vibrio cholera in patient's feces,
Check time minimum that result is negative.
Summary of the invention
The shortcoming of prior art in view of the above, it is an object of the invention to provide a kind of cholera vibrio O 139 gold colloidal detection
Test kit and its production and use, is used for solving the problems of the prior art.
For achieving the above object and other relevant purposes, the present invention is by the following technical solutions:
A first aspect of the present invention, it is provided that a kind of cholera vibrio O 139 gold-immunochromatographyreagent reagent for assay box, including test card, described examination
Paper card includes base plate and is positioned at the sample pad starting to be arranged in order from sample-adding end of backplate surface, gold mark pad, nitrocellulose filter
And adsorptive pads, described gold mark pad comprises anti-cholera vibrio O 139 type monoclonal antibody, described nitrocellulose filter is coated with
Detection line and nature controlling line, the anti-cholera vibrio O 139 type monoclonal antibody on described gold mark pad uses colloid gold label.
Preferably, the anti-cholera vibrio O 139 type monoclonal antibody on described gold mark pad uses 150nm colloid gold label, has
Signal is little by ambient interferences, and detection sensitivity is high, the advantage that result is reproducible.
Preferably, described base plate is PVC base plate.
Preferably, on described nitrocellulose filter, detection line is positioned at side close to sample-adding end, and nature controlling line is positioned at from sample-adding end relatively
Remote side.
Preferably, described detection line is coated with anti-cholera vibrio O 139 type monoclonal antibody.Anti-cholera on described gold mark pad
Vibrio O139 type monoclonal antibody can be identical antibody with the anti-cholera vibrio O 139 type monoclonal antibody on detection line, also
Can be different antibody.
Preferably, nature controlling line is coated sheep anti-mouse antibody.
Preferably, described sample pad use buffer process, described buffer selected from PBS, Tris-HCl buffer,
The combination of one or more in glycine buffer, borate buffer solution and citrate-phosphate salt buffer, the concentration of buffer
For 50-100mM.
Preferably, gold mark pad of the present invention is also through pretreatment, and the pre-treatment buffer used during pretreatment is selected from sweet ammonia
Acid buffer, Tris-HCl buffer, the combination of one or more of borate buffer solution, the concentration of buffer is 10-40mM.
Preferably, described buffer also includes increased response agent, described increased response agent selected from PEG4000, PEG6000,
Any one in PEG8000 and PEG20000, the concentration of described increased response agent is 10～50g/L.
Preferably, described buffer solution also includes surfactant, and described surfactant is selected from S-19TWEEN 20, S-20
In TWEEN 80, S-13TRITON X-45, S-14TRITON X-100, S-15TRITON X305 any one or
Multiple combination, the concentration of described surfactant is 20～40g/L.
Preferably, so that test kit has more preferably sensitivity and specificity, gold mark pad of the present invention is when pretreatment
The pre-treatment buffer used includes following component: fructose, aluminium hydroxide, glucose, sodium chloride and glycine, and fructose,
The total concentration of aluminium hydroxide, glucose, potassium chloride and glycine is 4.5-6.5g/L, and the pH value of buffer is 7.3-7.5.
Preferably, each component concentration in buffer is:
Fructose 1.0-2.0g/L；Aluminium hydroxide 0.25-0.75g/L；Glucose 1.0-1.5g/L；Sodium chloride 0.25-0.5g/L；Sweet
Propylhomoserin 2.0-2.25g/L.
The solvent of described pre-treatment buffer is water.
Concretely comprising the following steps of described pretreatment: gold mark pad soaks in pretreatment fluid 1.5～2h, takes out and is put in 36～38 DEG C of drying.
Described pre-treatment buffer can use the various conventional pH adjusting agent in this area to carry out the regulation of pH value.
Preferably, described test kit also includes getting stuck, described in get stuck and include that back card and upper cover, described back card are provided with test card draw-in groove,
Described test card is embedded in described test card draw-in groove, described on be covered with testing window and well, the position of described testing window and institute
Matching in the position stating detection line and nature controlling line, matches with the position of described sample pad in the position of described well.
Get stuck described in it is furthermore preferred that and get stuck for plastics.
Preferably, described detection kit is used for detecting cholera vibrio O 139.
Cholera vibrio O 139 gold-immunochromatographyreagent reagent for assay box provided by the present invention, can supporting immune quantitative analytical tool use.Exempt from
Epidemic disease analytical tool passes through acquisition testing line (T) and nature controlling line (C) band fluorescence signal, calculates T/C signal value.Use front elder generation
Being added drop-wise on test card by various criterion product, analyzing and processing sets up calibration curve (T/C signal value and the relation of standard substance actual value),
The T/C value obtained time again by detection sample compares with standard curve, can obtain the cholera vibrio O 139 antigen in detection sample
Content.
Second aspect present invention provides the preparation method of described cholera vibrio O 139 gold-immunochromatographyreagent reagent for assay box, comprises the steps:
1) with the anti-cholera vibrio O 139 type monoclonal antibody solution spraying gold mark pad of colloid gold label, prepare and comprise anti-cholera arc
The gold mark pad of bacterium O139 type monoclonal antibody；
2) on the detection line and nature controlling line of nitrocellulose filter, anti-cholera vibrio O 139 type monoclonal antibody and goat-anti are sprayed respectively
Murine antibody, prepares the nitrocellulose filter after being coated；
3) by sample pad, step 1) preparation gold mark pad, step 2) nitrocellulose filter prepared, adsorptive pads be pasted onto successively
On base plate, cutting prepares Test paper card；Finally Test paper is snapped fits into the prepared detection kit that gets stuck.
Preferably, gold mark pad of the present invention is also through pretreatment, and the pre-treatment buffer used during pretreatment is selected from sweet ammonia
Acid buffer, Tris-HCl buffer, the combination of one or more of borate buffer solution, the concentration of buffer is 10-40mM.
Preferably, described buffer also includes increased response agent, described increased response agent selected from PEG4000, PEG6000,
Any one in PEG8000 and PEG20000, the concentration of described increased response agent is 10～50g/L.
Preferably, described buffer solution also includes surfactant, and described surfactant is selected from S-19TWEEN 20, S-20
In TWEEN 80, S-13TRITON X-45, S-14TRITON X-100, S-15TRITON X305 any one or
Multiple combination, the concentration of described surfactant is 20～40g/L.
Preferably, so that test kit has more preferably sensitivity and specificity, gold mark pad of the present invention is when pretreatment
The pre-treatment buffer used includes following component: fructose, aluminium hydroxide, glucose, sodium chloride and glycine, and fructose,
The total concentration of aluminium hydroxide, glucose, potassium chloride and glycine is 4.5-6.5g/L, and the pH value of buffer is 7.3-7.5.
Preferably, each component concentration in buffer is:
Fructose 1.0-2.0g/L；Aluminium hydroxide 0.25-0.75g/L；Glucose 1.0-1.5g/L；Sodium chloride 0.25-0.5g/L；Sweet
Propylhomoserin 2.0-2.25g/L.
The solvent of described pre-treatment buffer is water.
Concretely comprising the following steps of described pretreatment: gold mark pad soaks in pretreatment fluid 1.5～2h, takes out and is put in 36～38 DEG C of drying.
Described pre-treatment buffer can use the various conventional pH adjusting agent in this area to carry out the regulation of pH value.
Third aspect present invention provides described cholera vibrio O 139 gold-immunochromatographyreagent reagent for assay box at cholera vibrio O 139 detection field
Purposes.
The invention have the benefit that
Cholera vibrio O 139 is passed through colloid gold immune by cholera vibrio O 139 gold-immunochromatographyreagent reagent for assay box provided by the present invention first
Chromatographic technique detects, and has high sensitivity and high specific concurrently, it is possible to quickly detect cholera vibrio O 139.Additionally, described inspection
Test agent box has the advantages such as operation is fast and convenient, result is accurate, economic and practical.
Detailed description of the invention
Below by way of specific instantiation, embodiments of the present invention being described, those skilled in the art can be by disclosed by this specification
Content understand other advantages and effect of the present invention easily.The present invention can also be added by the most different detailed description of the invention
To implement or application, the every details in this specification can also be based on different viewpoints and application, in the essence without departing from the present invention
Various modification or change is carried out under god.
Before further describing the specific embodiment of the invention, it should be appreciated that protection scope of the present invention is not limited to following specific
Specific embodiments；It is also understood that the term used in the embodiment of the present invention is to describe specific specific embodiments,
Rather than in order to limit the scope of the invention；In description of the invention and claims, unless literary composition additionally clearly refers to
Going out, singulative " ", " one " and " this " include plural form.
When embodiment provides numerical range, it should be appreciated that unless the present invention is otherwise noted, two end points of each numerical range with
And any one numerical value all can be selected between two end points.Unless otherwise defined, all technology used in the present invention and section are academic
The same meaning that language and those skilled in the art of the present technique are generally understood that.In addition to the concrete grammar used in embodiment, equipment, material,
According to those skilled in the art's grasp to prior art and the record of the present invention, it is also possible to use and the embodiment of the present invention
Described in method, any method, equipment and the material of the similar or equivalent prior art of equipment, material realize the present invention.
Unless otherwise indicated, the experimental technique that disclosed in this invention, detection method, preparation method all use the art normal
Rule molecular biology, biochemistry, chromatin Structure and analysis, analytical chemistry, cell cultivate, recombinant DNA technology and
The routine techniques of association area.These technology have improved explanation in existing document, specifically can be found in Sambrook etc.
MOLECULAR CLONING:A LABORATORY MANUAL, Second edition, Cold Spring Harbor
Laboratory Press, 1989and Third edition, 2001；Ausubel etc., CURRENT PROTOCOLS IN
MOLECULAR BIOLOGY, John Wiley&Sons, New York, 1987and periodic updates；the series
METHODS IN ENZYMOLOGY, Academic Press, San Diego；Wolffe, CHROMATIN
STRUCTURE AND FUNCTION, Third edition, Academic Press, San Diego, 1998；METHODS
IN ENZYMOLOGY, Vol.304, Chromatin (P.M.Wassarman and A.P.Wolffe, eds.), Academic
Press, San Diego, 1999；With METHODS IN MOLECULAR BIOLOGY, Vol.119, Chromatin
Protocols (P.B.Becker, ed.) Humana Press, Totowa, 1999 etc..
The preparation of embodiment 1 test card of the present invention
1) using pre-treatment buffer that gold mark pad is carried out pretreatment, pre-treatment buffer is: fructose 1.5g/L, aluminium hydroxide
0.5g/L, glucose 1.25g/L, sodium chloride 0.3g/L, the aqueous solution of glycine 2.0g/L, pH=7.4, the concrete step of pretreatment
Suddenly it is: gold mark pad is soaked in pretreatment fluid 2h, takes out and be put in 37 DEG C of drying；Then with the anti-cholera arc of colloid gold label
Bacterium O139 type monoclonal antibody solution spraying pretreated gold mark pad, prepares and is coated anti-cholera vibrio O 139 type monoclonal antibody
Gold mark pad, in solution, the mass ratio of gold colloidal and antibody is 5:1, and the concentration of solution is 10mg/ml, and quantity for spray is 4ul/cm；
2) on the detection line and nature controlling line of nitrocellulose filter, spray the anti-cholera vibrio O 139 type monoclonal of 1mg/ml respectively
Antibody-solutions and sheep anti-mouse antibody solution, quantity for spray is 1ul/cm, prepares the nitrocellulose filter after being coated；
3) by sample pad, step 1) preparation gold mark pad, step 2) nitrocellulose filter prepared, adsorptive pads be pasted onto successively
On PVC base plate, cutting prepares the Test paper card of wide 3-5mm；Finally Test paper is snapped fits into the prepared detection kit that gets stuck.
The preparation of embodiment 2 contrast agent box
The preparation of comparative example test card: using 25mM glycine buffer pretreatment gold mark pad, other reagent and experimental technique are equal
With embodiment 1.
1) 25mM glycine buffer pretreatment gold mark pad is used, then with the anti-cholera vibrio O 139 type list of colloid gold label
Clonal antibody solution spraying pretreated gold mark pad, prepares the gold mark pad being coated anti-cholera vibrio O 139 type monoclonal antibody,
In solution, gold colloidal is 5:1 with the mass ratio of antibody, and the concentration of solution is 10mg/ml, and quantity for spray is 4ul/cm；
2) on the detection line and nature controlling line of nitrocellulose filter, spray the anti-cholera vibrio O 139 type monoclonal of 1mg/ml respectively
Antibody-solutions and sheep anti-mouse antibody solution, quantity for spray is 1ul/cm, prepares the nitrocellulose filter after being coated；
3) by sample pad, step 1) preparation gold mark pad, step 2) nitrocellulose filter prepared, adsorptive pads be pasted onto successively
On PVC base plate, cutting prepares the Test paper card of wide 3-5mm；Finally Test paper is snapped fits into the prepared detection kit that gets stuck.
The specificity experiments of embodiment 3 detection kit
Detection method:
1. Example 1 preparation test kit of the present invention and the contrast agent box of embodiment 2, test kit is placed on level table.
2. the preparation of testing sample: by four class experimental subjecies of table 1, take from the different parts of tester's feces at random with special-purpose taking lining bar
Sample, sampling amount is as the criterion with the small circle ring being stained with special-purpose taking lining bar front end；Prepare a sample processing tube, be vertically put in process pipe
On frame, and in sample cell, add 0.6ml distilled water；The sample taken on special-purpose taking lining bar is inserted the distilled water in sample cell
Stir as testing sample.
3. every class experimental subject is 100 people, testing result such as table 1:
Table 1 detection kit specific test result
As seen from the above table, the detection kit of present invention no cross reaction equal to escherichia coli and dysentery bacterium, specificity is good.
In sum, detection kit provided by the present invention has good anti-interference and specificity, effectively overcomes existing
Various shortcoming in technology and have high industrial utilization.
The principle of above-described embodiment only illustrative present invention and effect thereof, not for limiting the present invention.Any it is familiar with this skill
Above-described embodiment all can be modified under the spirit and the scope of the present invention or change by the personage of art.Therefore, such as
All that in art, tool usually intellectual is completed under without departing from disclosed spirit and technological thought etc.
Effect is modified or changes, and must be contained by the claim of the present invention.
Claims (10)
1. a cholera vibrio O 139 gold-immunochromatographyreagent reagent for assay box, including test card, test card includes base plate and is positioned at backplate surface
The sample pad, gold mark pad, nitrocellulose filter and the adsorptive pads that start to be arranged in order from sample-adding end, described gold mark pad comprises
Anti-cholera vibrio O 139 type monoclonal antibody, described nitrocellulose filter is coated with detection line and nature controlling line, described gold mark
Anti-cholera vibrio O 139 type monoclonal antibody on pad uses colloid gold label.
2. gold-immunochromatographyreagent reagent for assay box as claimed in claim 1, it is characterised in that on described nitrocellulose filter, detection line is positioned at
Side close to sample-adding end, nature controlling line is positioned at from sample-adding end side farther out.
3. gold-immunochromatographyreagent reagent for assay box as claimed in claim 1, it is characterised in that be coated with anti-vibrio cholera on described detection line
O139 type monoclonal antibody.
4. gold-immunochromatographyreagent reagent for assay box as claimed in claim 1, it is characterised in that be coated sheep anti-mouse antibody on nature controlling line.
5. gold-immunochromatographyreagent reagent for assay box as claimed in claim 1, it is characterised in that described sample pad uses buffer to process, described
Buffer is selected from PBS, Tris-HCl buffer, glycine buffer, borate buffer solution and citrate-phosphate
The combination of one or more in salt buffer, the concentration of buffer is 50～100mM.
6. gold-immunochromatographyreagent reagent for assay box as claimed in claim 1, it is characterised in that described gold mark pad uses buffer to process, described
Buffer is selected from glycine buffer, Tris-HCl buffer, the combination of one or more of borate buffer solution, buffer
Concentration be 10～40mM.
Gold-immunochromatographyreagent reagent for assay box the most according to claim 6, it is characterised in that also include increased response agent in described buffer,
Any one in PEG4000, PEG6000, PEG8000 and PEG20000 of described increased response agent, described instead
The concentration answering reinforcing agent is 20～40g/L.
8. gold-immunochromatographyreagent reagent for assay box as claimed in claim 1, it is characterised in that also include getting stuck, described in get stuck include back card and
Upper cover, described back card is provided with test card draw-in groove, and described test card is embedded in described test card draw-in groove, described on be covered with test
Window and well, the position of described testing window matches with the position of described detection line and nature controlling line, the position of described well
Match with the position of described sample pad.
9. gold-immunochromatographyreagent reagent for assay box as claimed in claim 1, it is characterised in that described detection kit is used for detecting vibrio cholera
O139。
10., according to the preparation method of the gold-immunochromatographyreagent reagent for assay box described in claim 1～9 any claim, specifically include as follows
Step:
1) with the anti-cholera vibrio O 139 type monoclonal antibody solution spraying pretreated gold mark pad of colloid gold label, bag is prepared
Gold mark pad containing anti-cholera vibrio O 139 type monoclonal antibody；
2) on the detection line and nature controlling line of nitrocellulose filter, anti-cholera vibrio O 139 type monoclonal antibody and goat-anti are sprayed respectively
Murine antibody, prepares the nitrocellulose filter after being coated；
3) by sample pad, step 1) preparation gold mark pad, step 2) nitrocellulose filter prepared, adsorptive pads be pasted onto successively
On base plate, cutting prepares Test paper card；Finally Test paper is snapped fits into the prepared detection kit that gets stuck.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510242775.4A CN106290842A (en) | 2015-05-13 | 2015-05-13 | Cholera vibrio O 139 colloidal gold method detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510242775.4A CN106290842A (en) | 2015-05-13 | 2015-05-13 | Cholera vibrio O 139 colloidal gold method detection kit |
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| Publication Number | Publication Date |
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| CN106290842A true CN106290842A (en) | 2017-01-04 |
Family
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Family Applications (1)
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|---|---|---|---|
| CN201510242775.4A Pending CN106290842A (en) | 2015-05-13 | 2015-05-13 | Cholera vibrio O 139 colloidal gold method detection kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771171A (en) * | 2017-02-17 | 2017-05-31 | 中山大学 | A kind of fresh-water fishes encysted metacercaria of clonorchis sinensis infection colloidal gold fast detecting test paper strip and preparation method thereof |
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| CN1224165A (en) * | 1998-12-15 | 1999-07-28 | 中国预防医学科学院流行病学微生物学研究所 | One-step fast cholera diagnosis reagent box |
| WO1999064864A1 (en) * | 1998-06-12 | 1999-12-16 | New Horizons Diagnostics Inc. | Optimizing sensitivity in colloidal colorimetric flow through and lateral flow tests |
| WO2001031337A2 (en) * | 1999-10-27 | 2001-05-03 | Genosis Limited | Lateral flow device utilising particulate carrier |
| CN1866021A (en) * | 2006-05-16 | 2006-11-22 | 郑州理利生物工程有限公司 | Test paper for quick detection of cholera vibrio 01 group, 0139 group and cholera toxin |
| CN1963520A (en) * | 2005-11-10 | 2007-05-16 | 北京庄笛浩禾生物医学科技有限公司 | Rapid test paper bar for testing colloidal gold of 0139 group cholera vibrio |
| CN104360085A (en) * | 2014-12-05 | 2015-02-18 | 重庆乾德生物技术有限公司 | AFP (alpha fetal protein) detection kit |
-
2015
- 2015-05-13 CN CN201510242775.4A patent/CN106290842A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999064864A1 (en) * | 1998-06-12 | 1999-12-16 | New Horizons Diagnostics Inc. | Optimizing sensitivity in colloidal colorimetric flow through and lateral flow tests |
| CN1224165A (en) * | 1998-12-15 | 1999-07-28 | 中国预防医学科学院流行病学微生物学研究所 | One-step fast cholera diagnosis reagent box |
| WO2001031337A2 (en) * | 1999-10-27 | 2001-05-03 | Genosis Limited | Lateral flow device utilising particulate carrier |
| CN1963520A (en) * | 2005-11-10 | 2007-05-16 | 北京庄笛浩禾生物医学科技有限公司 | Rapid test paper bar for testing colloidal gold of 0139 group cholera vibrio |
| CN1866021A (en) * | 2006-05-16 | 2006-11-22 | 郑州理利生物工程有限公司 | Test paper for quick detection of cholera vibrio 01 group, 0139 group and cholera toxin |
| CN104360085A (en) * | 2014-12-05 | 2015-02-18 | 重庆乾德生物技术有限公司 | AFP (alpha fetal protein) detection kit |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771171A (en) * | 2017-02-17 | 2017-05-31 | 中山大学 | A kind of fresh-water fishes encysted metacercaria of clonorchis sinensis infection colloidal gold fast detecting test paper strip and preparation method thereof |
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Application publication date: 20170104 |