CN104964968A - Microbial time temperature indicator used for table grape quality perception - Google Patents
Microbial time temperature indicator used for table grape quality perception Download PDFInfo
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- CN104964968A CN104964968A CN201510243990.6A CN201510243990A CN104964968A CN 104964968 A CN104964968 A CN 104964968A CN 201510243990 A CN201510243990 A CN 201510243990A CN 104964968 A CN104964968 A CN 104964968A
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- time temperature
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- temperature indicator
- tti
- table grape
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Abstract
The present invention provides a microbial time temperature indicator used for table grape quality perception, which includes a container, the container contains an evenly-mixed microorganism microbial broth, a growth medium and a pH indicator; the microorganism is Lactobacillus paracasei bacilli; the growth medium is MRS bouillon culture-medium; and the pH indicator is bromocresol green. The activation energy of the time temperature indicator is close to the activation energy of fruits and vegetables, can be used to monitor quality changes of the fresh fruits and vegetables in cold chain logistics, and in particular, can be used for accurate quality perception and monitor of table grapes.
Description
Technical field
The present invention relates to Time-temperature-shelf life instruction and quality monitoring technical field, specifically, relate to a kind of microbiocidal time temperature indicator for Table Grape quality perception.
Background technology
Time temperature indicator (Time Temperature Indicator, TTI) be a kind of can record and the time-temperature course of following the tracks of in distributing fresh agricultural products process, and move mark and the cognition technology of all or part of shelf life of the directly monitored product of reflection by mechanical deformation, color change or color.Its monitoring result is more more reliable than marks such as traditional default shelf-life, at the latest selling periods, ensures consumption safety.According to principle of work, TTI can be divided into chemical type, enzyme type, microbiocidal, physical chemistry type, diffused, polymer-type, solid-state reaction type, photaesthesia type TTI.The research in past mainly concentrates on enzyme type, chemical type, diffused TTI, and little about the research of microbiocidal TTI.
Microbiocidal TTI is generally made up of microorganism, pH indicator, growth matrix.Relative to the TTI of other types, the maximum advantage of microorganism TTI is that its response process is a simulated foods feature spoilage organisms growth and metabolic process.This shows, the food that the fresh agricultural products quality that microorganism TTI causes due to spoilage organisms in monitoring reduces has higher accuracy.In addition, from economic point of view, what a large number of microorganism can be more cheap is obtained, and the cost of manufacture of microorganism TTI is low, and microorganism TTI can be impelled to be widely used in food quality monitoring.
The thin succulence of Table Grape skin, nutritious, very suitable micro-organisms growth, breeding, go mouldy, ash become and produce peculiar smell and toxicity, reduction edibility and economic worth greatly.China is the first in the world large Table Grape main product state, accounts for world's vintage 10.8%.The cold chain process of its specification is strict to temperature requirement: precooling requires-2 DEG C ~-1 DEG C, and refrigeration then requires 0 DEG C ~ 1 DEG C, and transport then requires to control below 5 DEG C; But also often apply the preservation techniques such as gas anti-corrosive fresh-keeping, Calcium treatment preservation technique and air conditioned storage.But because China's Table Grape technology of storage and transport falls behind, the loss that reduced by quality or go mouldy etc. causes is up to 25 ~ 30%.Therefore, improve Table Grape Cold Chain Logistics, reduce the garbage treatment quantity and unhelpful CO that rot to cause
2discharge, improves environmental protection and ecological benefits; Cut the waste and economic loss, promote the development of storage and transportation of grapes material flow industry, improve Industry Core Competitive, there is important social benefit.
Microorganism TTI, as a kind of intelligent packaging, can be applicable to Table Grape Cold Chain Logistics quality monitoring.Microorganism TTI is from Table Grape picks, whole transport, storage, dispensing, sale until consumer edible before, identical time-temperature history is experienced with Table Grape, the real-time remaining shelf life of Table Grape is characterized by color change, facilitate the sale of sellers to Table Grape to make decision-making timely and effectively, cut the waste and economic loss.
Its energy of activation scope of the microorganism TTI of current existence is 82.55 ~ 127.28kJ/mol, and its energy of activation difference compared with the energy of activation of Table Grape is comparatively large, can not the quality comparison of very accurate measurements Table Grape, and indicates its shelf life.When the energy of activation of microorganism TTI and Table Grape is more close, microorganism TTI indicates shelf life effect more accurate.The application improves the one of prior art on Table Grape quality monitoring, by improving formula, obtains the microorganism TTI prototype more close with Table Grape energy of activation.
Lactobacillus paraceasi is subordinate to lactobacillus, is a kind of probio.Decomposition glucose in growth metabolism process, produces lactic acid.Utilize this character can make microorganism TTI.Also do not utilize lactobacillus paraceasi to make microorganism TTI at present.
Summary of the invention
In order to solve problems of the prior art, the object of this invention is to provide a kind of microbiocidal time temperature indicator (TTI) for Table Grape quality perception.
In order to realize the object of the invention, first the present invention provides a kind of microbiocidal time temperature indicator for Table Grape quality perception, and it comprises a container, containing the microbial inoculum mixed, growth matrix and pH indicator in container; Described microorganism is lactobacillus paraceasi; Described growth matrix is MRS broth bouillon; Described pH indicator is bromcresol green.
Further, the preparation method of described time temperature indicator comprises the steps:
1) in growth matrix, add bromcresol green powder, obtain the MRS solution being mixed with pH indicator;
2) by microbial inoculum and step 1) the MRS solution that obtains mixes, to obtain final product.
As preferably, described step 1) be: in often liter of growth matrix, add 0.039 ~ 0.041g bromcresol green powder, obtain the MRS solution being mixed with pH indicator.
As preferably, described step 2) be: by microbial inoculum and step 1) the MRS solution that obtains with volume ratio for 10
-5: the ratio of 1 mixes.
Further, the preparation method of described microbial inoculum is specially: activated by lactobacillus paraceasi dry powder, 37 DEG C of constant temperature culture 24 hours, by centrifugal for bacterium liquid, wash twice by stroke-physiological saline solution, be precipitated, add stroke-physiological saline solution, being prepared into concentration is 10
-8~ 10
-9the microbial inoculum of CFU/ml; Wherein, when actication of culture and cultivation, nutrient culture media used is MRS broth bouillon.
Present invention also offers aforesaid time temperature indicator (TI) in Table Grape quality perception and the application in monitoring.
Table Grape is from picking, and namely stick this microorganism TTI label, Table Grape experiences identical time-temperature history with microorganism TTI.The whole transport of Table Grape, storage, dispensing, sale until consumer edible before in process, the color of microorganism TTI label constantly changes along with the accumulation of time-temperature, the quality of Table Grape is also along with the accumulation of time-temperature constantly reduces, when the color of microorganism TTI arrives the color of shelf life terminal, mean that Table Grape quality is reduced to not edible.
Beneficial effect of the present invention is:
The present invention utilizes lactobacillus paraceasi, growth matrix and bromcresol green indicator solution to prepare a kind of time temperature indicator.Lactobacillus paraceasi is a kind of probio, and growth matrix directly can adopt the MRS broth bouillon of standardized production on market, convenient and swift.This microbiocidal time temperature indicator cost of manufacture is cheap, and method is simple, is conducive to large-scale production.
The energy of activation of its energy of activation of time temperature indicator provided by the invention and fruits and vegetables is close, and can be used for monitoring the quality comparison in fresh fruits and vegetables Cold Chain Logistics, especially can accurately for Table Grape quality perception and monitoring.Relative to the microorganism TTI existed at present, this microorganism TTI energy of activation and Table Grape energy of activation more close, therefore indicating effect is more accurate.Relative to the temperature monitoring technique based on WSN existed at present, this technology has that cost is low, volume is little, without the need to the feature of Power supply.In addition, this microorganism TTI has the quality comparison of Real-Time Monitoring Table Grape and predicts the feature of shelf life, facilitates the sale of sellers to Table Grape to make decision-making timely and effectively, avoids waste and economic loss.
Accompanying drawing explanation
Fig. 1 is the pictorial diagram of time temperature indicator of the present invention.
Fig. 2 is the color change course of time temperature indicator of the present invention under 15 DEG C of constant temperatures.
Fig. 3 is titratable acid concentration (TA) changing trend diagram of time temperature indicator of the present invention under 15 DEG C of constant temperatures.
Fig. 4 is the total color difference Δ E changing trend diagram of time temperature indicator of the present invention under 15 DEG C of constant temperatures.
Fig. 5 is the pH changing trend diagram of time temperature indicator of the present invention under 15 DEG C of constant temperatures.
Fig. 6 is the pH changing trend diagram of time temperature indicator of the present invention under 25 DEG C of constant temperatures.
Fig. 7 is the pH changing trend diagram of time temperature indicator of the present invention under 37 DEG C of constant temperatures.
Fig. 8 is the Arrhenius figure of pH in time temperature indicator system of the present invention.
Fig. 9 is the Arrhenius figure of TA in time temperature indicator system of the present invention.
Figure 10 is the Arrhenius figure of Δ E in time temperature indicator system of the present invention.
Figure 11 is the Arrhenius figure of the hardness of rose fragrant Table Grape.
Figure 12 is the Arrhenius figure of the percentage of water loss of rose fragrant Table Grape.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
Prepare lactobacillus paraceasi bacterium liquid: lactobacillus paraceasi dry powder is carried out twice activation, and then cultivate 24 hours at 37 DEG C, by centrifugal for bacterium liquid, wash twice by stroke-physiological saline solution, be precipitated, add stroke-physiological saline solution, being prepared into concentration is 10
-8~ 10
-9the microbial inoculum of CFU/ml, for subsequent use.
Preparation growth matrix: preparation MRS cultured solution of broth.
Preparation is mixed with the MRS solution of pH indicator: often liter of MRS broth bouillon adds the bromcresol green powder of 0.04g.
The MRS solution of described microbial inoculum and the described pH of being mixed with indicator with volume ratio for 10
-5: 1 mixes, and is distributed into container, i.e. obtained microorganism TTI.
Practicality measures:
At this microorganism TTI is placed in 15 DEG C, measures response (titratable acid concentration, pH, total Se Cha ⊿ E) every about 4h, obtain TTI response parameter changing trend diagram.
The mensuration application Hunter Lab system of TTI color, Minolta CR410 colour difference meter is adopted to measure L, a, b value, wherein L represents brightness, a represents a axle value in Hunter (Hunter) scale, and b represents the b axle value in Hunter (Hunter) scale, and+a represents red,-a represents green, + b represents yellow, and-b represents that blue ， ⊿ E represents total color difference (aberration refer to represent that two kind colors to difference people color perception on numerical value).
E＝(⊿a
2+⊿b
2+⊿c
2)
1/2
The sterile distilled water dilution of the TTI solution 20ml of the measurement of TTI titratable acid concentration: 5g, makes its homogenize (10s) with the phenolphthalein of 1% of 0.5ml, is titrated to pH=8.2 afterwards with the NaOH of 0.1mol/L.
TA(％)＝a*b*0.009*100/w
A is the consumption of NaOH, and unit is ml; B is the volumetric molar concentration of NaOH; 0.009 is the equivalence factor of lactic acid; W is example weight (g).
Fig. 2 is under 15 DEG C of constant temperatures, and the color change course of this indicator TTI system, as can be seen from Figure 2, along with the migration of time.This indicator TTI creates the obvious color change from blackish green to yellow.
Fig. 3 is that in the reaction of this indicator TTI system under 15 DEG C of constant temperatures, titratable acid concentration change trend map, as can be seen from Figure 3, along with the increase of time, titratable acid concentration is in rising trend.
Fig. 4 is that in the reaction of this indicator TTI system under 15 DEG C of constant temperatures, total color difference Δ E changing trend diagram, as can be seen from Figure 4, along with the increase of time, total color difference is in rising trend.
Fig. 5 is that in the reaction of this indicator TTI system under 15 DEG C of constant temperatures, pH changing trend diagram, as can be seen from Figure 5, along with the increase of time, pH is on a declining curve.
As can be seen from Fig. 3 ~ 5, in the reaction of this indicator TTI system under 15 DEG C of constant temperatures, titratable acid concentration, total color difference Δ E, pH all present good linear relationship with time t.
Prepare microbiocidal time temperature indicator according to the method for embodiment, under 15 DEG C, 25 DEG C, 37 DEG C conditions, carry out constant temperature test respectively.The total color difference of periodic measurement time temperature indicator, pH, titratable acid concentration.
Fig. 5 ~ 7 are in the reaction of this indicator TTI system under 15 DEG C, 25 DEG C, 37 DEG C constant temperatures, the changing trend diagram of pH, as can be seen from the figure, this reaction system is at different temperatures, pH all in good linear relationship, can obtain the reaction rate under different temperatures over time.
According to Arrhenius equation: – lnk=E
a/ RT-lnk
0, get natural logarithm by obtaining reaction rate in Fig. 3 ~ 7, then carry out linear fit with the inverse of absolute temperature and gas law constant product, opening relationships curve lnk-1/RT, the Arrhenius figure of time temperature indicator pH, TA, ⊿ E can be drawn, as Fig. 8 ~ 10.The each index energy of activation of TTI is directly obtained by straight slope.PH, TA, ⊿ E energy of activation of TTI is followed successively by 76.052kJ/mol, 79.461kJ/mol, 73.926kJ/mol.
In like manner, the crucial index of quality hardness of rose fragrant Table Grape and percentage of water loss are measured, obtain its Arrhenius curve, as Figure 11 ~ 12, the hardness of rose fragrant Table Grape can be obtained by figure, the energy of activation of the percentage of water loss index of quality is 66.905kJ/mol, 71.955kJ/mol.Wherein the percentage of water loss of Table Grape and microorganism TTI ⊿ E energy of activation scope are all at 70 ~ 75kJ/mol, and difference 1.971kJ/mol, the most close.TTI differs 20kJ/mol with the energy of activation of product in theory, and TTI can accurately monitor for this product quality.Therefore, this microbiocidal time temperature indicator can be used for Table Grape quality perception and monitoring.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (6)
1. for a microbiocidal time temperature indicator for Table Grape quality perception, it is characterized in that, it comprises a container, containing the microbial inoculum mixed, growth matrix and pH indicator in container; Described microorganism is lactobacillus paraceasi; Described growth matrix is MRS broth bouillon; Described pH indicator is bromcresol green.
2. time temperature indicator according to claim 1, is characterized in that, its preparation method comprises the steps:
1) in growth matrix, add bromcresol green powder, obtain the MRS solution being mixed with pH indicator;
2) by microbial inoculum and step 1) the MRS solution that obtains mixes, to obtain final product.
3. time temperature indicator according to claim 2, is characterized in that, described step 1) be: in often liter of growth matrix, add 0.039 ~ 0.041g bromcresol green powder, obtain the MRS solution being mixed with pH indicator.
4. the time temperature indicator according to Claims 2 or 3, is characterized in that, described step 2) be: by microbial inoculum and step 1) the MRS solution that obtains with volume ratio for 10
-5: the ratio of 1 mixes.
5. the time temperature indicator according to any one of claims 1 to 3, it is characterized in that, the preparation method of described microbial inoculum is specially: activated by lactobacillus paraceasi dry powder, 37 DEG C of constant temperature culture 24 hours, by centrifugal for bacterium liquid, wash twice by stroke-physiological saline solution, be precipitated, add stroke-physiological saline solution, being prepared into concentration is 10
-8~ 10
-9the microbial inoculum of CFU/ml; Wherein, when actication of culture and cultivation, nutrient culture media used is MRS broth bouillon.
6. the time temperature indicator described in any one of Claims 1 to 5 is in Table Grape quality perception and the application in monitoring.
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| CN105651771A (en) * | 2016-01-11 | 2016-06-08 | 中国农业大学 | Agricultural-product quality monitoring method with integration of color sensing and time-temperature indication |
| CN108037116A (en) * | 2017-11-14 | 2018-05-15 | 中国农业大学 | A kind of microorganism type time-temperature indicator card and its preparation method and application |
| CN110777187A (en) * | 2019-10-18 | 2020-02-11 | 江南大学 | Microorganism type time-temperature indicator |
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| CN104964968B (en) | 2018-04-13 |
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