CN103728280A - Surface plasma resonance coupling optical element - Google Patents
Surface plasma resonance coupling optical element Download PDFInfo
- Publication number
- CN103728280A CN103728280A CN201410018681.4A CN201410018681A CN103728280A CN 103728280 A CN103728280 A CN 103728280A CN 201410018681 A CN201410018681 A CN 201410018681A CN 103728280 A CN103728280 A CN 103728280A
- Authority
- CN
- China
- Prior art keywords
- optical element
- surface plasma
- optical
- resonant vibration
- plasma body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention relates to a surface plasma resonance coupling optical element. The optical element comprises a soft cavity, two optical windows, a sensing chip and a sample flowing cell, wherein the two optical windows are respectively installed at two opposite openings of the soft cavity in a sealing manner and are respectively used as an exciting light incident optical window and a reflected light emergent optical window of the surface plasma resonance coupling optical element, the sensing chip is installed at the center opening of the soft cavity in the sealing manner so as to form a sealing hollow cavity; the optical element also comprises optical transparent liquid filling the sealing hollow cavity, and the sample flowing cell is arranged on the sensing chip. The optical element has the advantages of keeping the exciting light to be always vertical to the incident optical window and keeping the reflected light to be always vertical to the emergent optical window, not needing to correct the angle, not using binding oil for fixing the sensing chip, and reducing interference of reflect of multiple interfaces, can be widely used by surface plasma resonance systems and surface plasma resonance imaging systems.
Description
Technical field
The present invention relates to a kind of surface plasma body resonant vibration and imaging analysis instrument, particularly about a kind of surface plasma body resonant vibration coupling optical element.
Background technology
Surface plasma body resonant vibration is a kind of mark, real-time interface analysis technology of original position exempted from.The occurrence condition of surface plasma body resonant vibration changes with the variation of sample refractive index on sensing membrane-medium interface.Therefore by surface plasma body resonant vibration, analyze, can obtain refractive index and the thickness information of sensing membrane surface sample, can the physics on sensing membrane surface and chemical reaction be monitored and be analyzed, if target molecule is bonded in to sensing membrane surface, can carry out specific molecular transactional analysis.Based on Applications of surface plasmon resonance, develop surface plasma body resonant vibration and surface plasma resonance image-forming two alanysis systems, be all widely used in the fields such as chemistry, biology, medical science, physics, material.Especially surface plasma resonance image-forming, combines with biochip technology, by sensing membrane finishing different probe molecular array, can realize high-throughout detection or screening to the object in testing sample.In recent years along with the further investigation of the various set of biomolecules such as genomics, proteomics, surface plasma resonance image-forming more and more comes into one's own, and is considered to the very potential high flux biochemical analysis method of one.
Current surface plasma body resonant vibration and surface plasma resonance image-forming system are to carry out excitating surface plasma resonance based on gram Lay Schumann (Kretschmann) structure mostly, and its general structure schematic diagram as shown in Figure 1.Gram Lay Schumann (Kretschmann) structure is generally based on first-class girdle prism 1, sensing membrane 2 is arranged on etc. on the bottom surface 3 of girdle prism 1, optical excitation element 4 such as is positioned at first central plane 5 one sides of girdle prism 1, from the exciting light 6 of optical excitation element 4 outgoing, from the first central plane 5 that waits girdle prism 1, inject prism 1, on bottom surface 3, there is total reflection, reflected light 7 waits girdle prisms 1 from the second central plane 8 ejaculations, by the photodetector 9 of the second central plane 8 one sides of girdle prisms 1 such as being positioned at, is detected.The optical axis intersection of optical excitation element 4 and photodetector 9 is in the central spot of the bottom surface 3 of girdle prism 1 such as grade.When carrying out optical excitation angle scanning, optical excitation element 4 and photodetector 9 simultaneously around the central point of bottom surface 3 that waits girdle prism 1 carry out relative to or contrary rotating.
There are some shortcomings in this surface plasma body resonant vibration coupling element based on prism:
1. the shape of prism is fixed, excitation line can not be vertical with the plane of incidence of prism all the time, same reflection ray can not be vertical with the exit facet of prism all the time, when optical excitation Angulation changes, due to photorefractive effect, excite facula position to be offset, cause that light distribution changes, thus impact analysis effect; But also can bring the Correction Problems of optical excitation angle.
In order to reduce costs, easy to use, sensing membrane is prepared on substrate more, then by the oil that bonds, is affixed on the bottom surface of prism, the processing ease that bonds oily produces bubble, thereby affects image quality and analytical effect.
3. the exit facet of the plane of incidence, reflection ray and the prism of excitation line and prism can not remain vertical, and the refractive index of prism, bonding oil and substrate is difficult to perfect match, will bring the reflection interference problem at many interfaces.
Above-mentioned all problems, except meeting brings the trouble of angularity correction, also can have a strong impact on the analysis quality of surface plasma body resonant vibration and surface plasma resonance image-forming system.
If therefore can build a kind of surface plasma body resonant vibration coupling optical element, overcome above-mentioned shortcoming, will be extremely important and significant.
Summary of the invention
The object of the invention is to propose a kind of deformation and be easy to the surface plasma body resonant vibration coupling optical element of controlling, have can keep excitation line and beam incident optical window, reflection ray and outgoing optical window all the time vertical, without angularity correction, sensing chip fixing without use bond oily, reduce the advantages such as many boundary reflections interference.The present invention can be widely used in surface plasma body resonant vibration system and surface plasma resonance image-forming system.
The present invention is achieved through the following technical solutions:
A kind of surface plasma body resonant vibration coupling optical element, is characterized in that: described optical element comprises a soft cavity, two optical windows, a sensing chip, a sample flow cell; Described two optical windows are seal-installed on respectively two relative openings of soft cavity, are used separately as exciting light beam incident optical window and the reflected light outgoing optical window of surface plasma body resonant vibration coupling optical element; Described sensing chip is seal-installed on the central opening of soft cavity; Described sample flow cell is positioned on sensing chip.
According to the present invention, it is characterized in that, described optical element also comprises optically transparent liquid.
According to the present invention, described sensing chip comprises an optical transparent substrate and preparation sensing membrane thereon.Described optical transparent substrate directly contacts with optically transparent liquid; Described sensing membrane directly contacts with sample flow cell.
According to the present invention, described sensing membrane is by conventional method, and the method for manufacturing thin film such as such as vacuum evaporation are directly prepared on substrate.
According to the present invention, described soft cavity has a central opening, is positioned at two relative openings of central opening both sides, and two apertures.
According to the present invention, described two apertures lay respectively at bottom and the top of soft cavity.It is used separately as input hole and the delivery outlet of described optically transparent liquid.
According to the present invention, described soft cavity can be that deformation is easy to the materials such as the rubber, dimethyl silicone polymer (PDMS) controlled and makes.
According to the present invention, described optical window can be that optically transparent glass, quartz, silicon, plastic or other material are made.
According to the present invention, described optically transparent liquid can be water, oil, ionic liquid etc. various pure or mix liquid.
According to the present invention, described optical transparent substrate can be glass, quartz, silicon, plastics etc.; Described sensing membrane is the material that surface plasma body resonant vibration can occur gold, silver etc., can to sensing membrane, carry out functionalized modification as required.
The present invention also provides a kind of application of surface plasma body resonant vibration coupling optical element, it is characterized in that, described element is used for surface plasma body resonant vibration system, the surface plasma resonance image-forming system of angle scanning, or in the surface plasma body resonant vibration system and surface plasma resonance image-forming system of length scanning.
A kind of surface plasma body resonant vibration coupling optical element of the present invention, during use, sensing chip position is fixed, and two optical window positions of both sides are adjustable, have the following advantages:
1. when surface plasma body resonant vibration optical excitation Angulation changes, excitation line can guarantee vertical with beam incident optical window all the time, same reflection ray also can guarantee vertical with outgoing optical window all the time, surface plasma body resonant vibration excites facula position constant all the time, has avoided the conventional prism light changes in distribution that excites spot displacement to cause of bringing that is coupled; And without carrying out exciting light angularity correction.
2. the substrate of sensing chip directly contacts with optically transparent liquid, without using bonding oil, has avoided the processing ease that bonds oily to produce air bubble problem.
3. reduced the reflection interference at many interfaces.
4. can freely change the optically transparent liquid that is positioned at soft cavity, select the liquid of appropriate index, can further improve detection sensitivity.
Therefore, a kind of surface plasma body resonant vibration coupling optical element proposed by the invention, can effectively improve analysis quality, the raising detection sensitivity of surface plasma body resonant vibration and surface plasma resonance image-forming system, and save the trouble of angularity correction and bonding oil operation.The present invention can directly apply in the surface plasma body resonant vibration and surface plasma resonance image-forming system of exciting light and reflected light angle linkage, only sensing membrane need be fixed on the rotating shaft extended line of exciting light motion and reflected light motion, two optical windows are separately fixed on corresponding exciting light and reflected light motion.
Accompanying drawing explanation
Fig. 1 is gram surface plasma body resonant vibration of Lay Schumann structure or the general structure schematic diagram of surface plasma resonance image-forming system.
Fig. 2 is the structural representation of surface plasma body resonant vibration coupling optical element of the present invention.
Fig. 3 A, B are the use view that adopts surface plasma body resonant vibration coupling optical element of the present invention.
Fig. 4 is the bovine serum albumin(BSA) (BSA) and the interactional surface plasma body resonant vibration curve map of anti-BSA that adopts surface plasma body resonant vibration coupling optical element of the present invention.
Wherein, the implication of each Reference numeral is as follows:
1: wait girdle prism; 2: sensing membrane; 3: bottom surface; 4: optical excitation element; 5: the first central planes; 6: exciting light; 7: reflected light; 8: the second central planes; 9: photodetector; 10: soft cavity; 11,12: optical window; 13: sensing chip; 14: sample flow cell; 15: optically transparent liquid; 16: central opening; 17,18: opening; 19,20: aperture; 21,22: fixture; 23: optical transparent substrate; 24: elastomeric pad; 25: sensing chip fixed mount; 26: glass sheet; 27: elastomeric pad; 28,29: aperture; 30: exciting light motion; 31: reflected light motion.
Embodiment
Below in conjunction with accompanying drawing and exemplary embodiments, the specific embodiment of the present invention is described in detail.But those skilled in the art understand, and following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.Those skilled in the art can modify and equivalent transformation according to instructions of the present invention, claims and accompanying drawing, and such modification all should not got rid of outside scope of the present invention with conversion.Any improvement of making on basis of the present invention and variation, all within protection scope of the present invention.
Structural representation of the present invention as shown in Figure 2.Described a kind of surface plasma body resonant vibration coupling optical element comprises 10, two optical windows of a soft cavity, 11,12, one sensing chip 13, one sample flow cells 14, and optically transparent liquid 15.
Described soft cavity 10 has a central opening 16, is positioned at two relative openings 17,18 of central opening both sides, and two apertures 19,20; Described soft cavity can be easy to the materials such as the rubber, dimethyl silicone polymer (PDMS) controlled and make by deformation, adopt in the present embodiment elastic silicone rubber pipe to be processed into.
Described two optical windows 11,12 are seal-installed on respectively two relative openings 17,18 of soft cavity 10, are used separately as the beam incident optical window of exciting light 6 and the outgoing optical window of reflected light 7 of surface plasma body resonant vibration coupling optical element; Described two optical windows 11,12 can be that optically transparent glass, quartz, silicon, plastic or other material are made, and in the present embodiment, are two blocks of circular K9 glass, and are separately fixed on aluminum fixture 21,22.
Described sensing chip 13 is installed on the central opening 16 of soft cavity 10, comprises an optical transparent substrate 23 and preparation sensing membrane 2 thereon.In the present embodiment, optical transparent substrate 23 is the K9 glass sheet of a thickness 0.15mm, and sensing membrane 2 is the golden film of thickness 50nm.Sensing chip 13 is fixed on sensing chip fixed mount 25 by pressure seal through PDMS elastomeric pad 24.Sensing chip fixed mount 25 is seal-installed on the central opening 16 of soft cavity 10.The optical transparent substrate 23 of sensing chip 13 directly contacts with optically transparent liquid 15, and sensing membrane 2 directly contacts with sample flow cell 14.
Described sample flow cell 14 is that a K9 glass sheet 26 is realized sealing by PDMS elastomeric pad 27 and sensing chip 13 by pressure; On K9 glass sheet 26, have two apertures 28,29, as the sample of sample flow cell, input and delivery outlet respectively.
Described two apertures 19,20 lay respectively at bottom and the top of soft cavity 10, are used separately as input and the delivery outlet of described optically transparent liquid 15.
Surface plasma body resonant vibration coupling optical element of the present invention, during use, fixing of sensing chip fixed mount 25 passed through in the position of sensing chip 13, and the position of two optical windows 11,12 of both sides is adjustable.Therefore, when surface plasma body resonant vibration optical excitation Angulation changes, excitation line 6 can guarantee vertical with beam incident optical window 11 all the time, same reflection ray 7 also can guarantee vertical with outgoing optical window 12 all the time, surface plasma body resonant vibration excites facula position constant all the time, has avoided the conventional prism light changes in distribution that excites spot displacement to cause of bringing that is coupled; And without carrying out exciting light angularity correction.The substrate 23 of sensing chip 13 directly contacts with optically transparent liquid 15, without using bonding oil, has avoided the processing ease that bonds oily to produce air bubble problem.The present invention has reduced the reflection interference at many interfaces.In addition, the optically transparent liquid 15 that is positioned at soft cavity 10 can freely be changed, if select the liquid of appropriate index, will further improve detection sensitivity.
The present invention can directly apply in the surface plasma body resonant vibration and surface plasma resonance image-forming system of exciting light and reflected light angle linkage.As shown in Fig. 3 A, B, only two optical window fixtures 21,22 need be separately fixed on corresponding exciting light motion 30 and reflected light motion 31, sensing membrane 2 be fixed on the rotating shaft extended line of exciting light motion 30 and reflected light motion 31.Optical excitation element 4 and beam incident optical window 11 are all fixed on exciting light motion 30, photodetector 9 and outgoing optical window 12 are all fixed on reflected light motion 31, by exciting light motion 30 and reflected light motion 31, link around the shaft and regulate Exciting incidence degree, it is vertical that excitation line 6 and beam incident optical window 11 can guarantee all the time, and same reflection ray 7 also can guarantee vertical with outgoing optical window 12 all the time.The present invention can be widely used in surface plasma resonance system and the surface plasma resonance image-forming system of angle scanning, also can be applied to surface plasma body resonant vibration system and the surface plasma resonance image-forming system of length scanning.
Fig. 4 is the bovine serum albumin(BSA) (BSA) and anti-BSA(anti-BSA that adopts surface plasma body resonant vibration coupling optical element of the present invention) interactional surface plasma body resonant vibration curve map.In sensing membrane 2, modified BSA probe molecule, optically transparent liquid 15 is 1-ethyl-3-methyl-iodonium imidazolide salts ionic liquid.In Fig. 4, first to passing into phosphoric acid buffer (PB) in sample flow cell, then pass into Anti-BSA solution 112 minutes time, reacting after a period of time with the BSA probe molecule in sensing membrane 2, in the time of 174 minutes, pass into again Anti-BSA solution, finally in the time of 192 minutes, pass into PB, wash away unconjugated Anti-BSA.The surface plasma body resonant vibration of having realized BSA and anti-BSA interphase interaction detects in real time.
Claims (9)
1. a surface plasma body resonant vibration coupling optical element, is characterized in that: described optical element comprises a soft cavity, two optical windows, a sensing chip, a sample flow cell; Described two optical windows are seal-installed on respectively two relative openings of soft cavity, are used separately as exciting light beam incident optical window and the reflected light outgoing optical window of surface plasma body resonant vibration coupling optical element; Described sensing chip is seal-installed on the central opening of soft cavity; Described sample flow cell is positioned on sensing chip.
2. according to the optical element of claim 1, it is characterized in that, described optical element also comprises optically transparent liquid.
3. according to the optical element of claim 1 or 2, it is characterized in that, described soft cavity has a central opening, is positioned at two relative openings of central opening both sides, and two apertures.
4. according to the optical element of claim 3, it is characterized in that, described two apertures lay respectively at bottom and the top of soft cavity, and described two apertures are used separately as input hole and the delivery outlet of described optically transparent liquid.
5. according to the optical element of claim 1-4 any one, it is characterized in that: described sensing chip comprises an optical transparent substrate and preparation sensing membrane thereon.Described optical transparent substrate directly contacts with optically transparent liquid; Described sensing membrane directly contacts with sample flow cell.
6. according to the optical element of claim 1-5 any one, it is characterized in that, described soft cavity is selected from rubber, dimethyl silicone polymer (PDMS).
7. according to the optical element of claim 1-6 any one, it is characterized in that, described optical window is selected from optically transparent glass, quartz, silicon or plastics.
Described optical transparent substrate is selected from glass, quartz, silicon or plastics.
Described sensing membrane is selected from gold or silver.
8. according to the optical element of claim 2, it is characterized in that: described optically transparent liquid is selected from water, oil, ionic liquid and their potpourri thereof.
9. the application of the surface plasma body resonant vibration coupling optical element of a claim 1-8 any one, it is characterized in that, described element is used for surface plasma body resonant vibration system, the surface plasma resonance image-forming system of angle scanning, or in the surface plasma body resonant vibration system and surface plasma resonance image-forming system of length scanning.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410018681.4A CN103728280B (en) | 2014-01-15 | 2014-01-15 | A kind of surface plasma body resonant vibration coupling optical element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410018681.4A CN103728280B (en) | 2014-01-15 | 2014-01-15 | A kind of surface plasma body resonant vibration coupling optical element |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103728280A true CN103728280A (en) | 2014-04-16 |
| CN103728280B CN103728280B (en) | 2016-01-20 |
Family
ID=50452449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410018681.4A Active CN103728280B (en) | 2014-01-15 | 2014-01-15 | A kind of surface plasma body resonant vibration coupling optical element |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103728280B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2042602U (en) * | 1988-12-22 | 1989-08-09 | 贾维波 | Angle-changing fluid-filled prism |
| US5164589A (en) * | 1988-11-10 | 1992-11-17 | Pharmacia Biosensor Ab | Reusable optical interface for non-permanent passive light coupling |
| US20060072113A1 (en) * | 2002-12-25 | 2006-04-06 | Boaz Ran | Surface plasmon resonance sensor |
| CN102590147A (en) * | 2012-02-01 | 2012-07-18 | 中国科学院化学研究所 | Surface plasma resonance system capable of adjusting sensitivity and dynamic range and detection method thereof |
-
2014
- 2014-01-15 CN CN201410018681.4A patent/CN103728280B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5164589A (en) * | 1988-11-10 | 1992-11-17 | Pharmacia Biosensor Ab | Reusable optical interface for non-permanent passive light coupling |
| CN2042602U (en) * | 1988-12-22 | 1989-08-09 | 贾维波 | Angle-changing fluid-filled prism |
| US20060072113A1 (en) * | 2002-12-25 | 2006-04-06 | Boaz Ran | Surface plasmon resonance sensor |
| CN102590147A (en) * | 2012-02-01 | 2012-07-18 | 中国科学院化学研究所 | Surface plasma resonance system capable of adjusting sensitivity and dynamic range and detection method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103728280B (en) | 2016-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6417925B1 (en) | Surface plasmon sensor for analyzing liquid sample or humid atmosphere | |
| US6862398B2 (en) | System for directed molecular interaction in surface plasmon resonance analysis | |
| CN102590147B (en) | Surface plasma resonance system capable of adjusting sensitivity and dynamic range and method thereof | |
| US9829434B2 (en) | Optical detection system for liquid samples | |
| CN106226278A (en) | A kind of multiplexing flow-through assay device for microlayer model fluoroscopic image and spectral scan | |
| JP2009162754A (en) | Measuring chip | |
| WO2004013616A1 (en) | Microchip, method for manufacturing microchip, and method for detecting component | |
| US20100061892A1 (en) | Microfluidic device having an array of spots | |
| WO2021185091A1 (en) | Detection chip and modification method therefor | |
| CN102519914B (en) | Wavelength modulation surface plasmon resonance detector based on laser confocal imaging | |
| US20090325315A1 (en) | Detection method of target substance, detection reagent used for the same, and the uses thereof | |
| US8106368B2 (en) | Fluorescence detecting method | |
| CN103868889B (en) | Micro cantilever beam array biochemical sensing device based on micro-mirror scanning and method | |
| CN103234961A (en) | Micro-cantilever beam array biochemical sensing apparatus and biochemical detection method thereof | |
| US20060127278A1 (en) | System and method of measuring molecular interactions | |
| TWI247886B (en) | Improved linear wave-guide type surface plasmon resonance micro sensor | |
| CN103698304B (en) | A kind of scissors liquid core coupled surface plasmons resonance imaging analyzer | |
| CN105548011A (en) | Micro-cantilever array biochemical sensing device and method based on optical fiber array | |
| US6870237B1 (en) | Repeated structure of nanometer thin films with symmetric or asymmetric configuration for SPR signal modulation | |
| CN100575927C (en) | Resonance imager of light-exciting color surface piasma | |
| US20210010920A1 (en) | Spectroscopic analysis device, spectroscopic analysis method, program, recording medium, and microscope | |
| IE20170055A1 (en) | Surface plasmon resonance sensor device using microfluidic channel and system comprising the same | |
| CN102466624A (en) | Microchip used for surface plasma resonance measurement and surface plasma resonance measuring device | |
| WO2021185087A1 (en) | Detection chip and modification method therefor | |
| Wei et al. | All-fiber biological detection microfluidic chip based on space division and wavelength division multiplexing technologies |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |