CN103691154B - A kind of chromatographic column for protein purification - Google Patents
A kind of chromatographic column for protein purification Download PDFInfo
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- CN103691154B CN103691154B CN201310644051.3A CN201310644051A CN103691154B CN 103691154 B CN103691154 B CN 103691154B CN 201310644051 A CN201310644051 A CN 201310644051A CN 103691154 B CN103691154 B CN 103691154B
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Abstract
The invention provides a kind of chromatographic column for protein purification, belong to biologically pure instrument field. Chromatographic column provided by the invention, arranges scale on cylinder, the grasp of handled easily personnel to packed height and sample size; With the setting of three silica gel hoses of inboard wall of cylinder, be convenient to the liquid such as buffer solution, sample liquid, eluent and flow down from the wall of cylinder, avoid causing and splash. The present invention is simple in structure, easy to operate, and the purification effect of albumen is good.
Description
Technical field
The invention belongs to biologically pure apparatus, be specifically related to a kind of chromatographic column for protein purification.
Background technology
At present, the separation and purification of the composition of multiple compounds, natural products and Chinese medicine, is mainly to adopt column chromatography, uses chromatographic column, realizes by column chromatography process. traditional column chromatography is the top that mixing cpd is placed on to glass column post bed, make the eluant, eluent (mobile phase) of mixed solvent composition under Action of Gravity Field, by the vertical glass column of absorbent media material (fixing phase) is housed, separation obtains each purer product (sterling), its principle is: because each component in mixing cpd is to fixing different with the affinity of mobile phase mutually in chromatographic column, therefore select suitable fixing phase and mobile phase according to the character of mixing cpd, can reach the object that wherein each component separates as far as possible, the product separating is collected into respectively in each receiving flask in pillar outlet, then, identify the chromatographic solution of collecting in each receiving flask, merge and concentrated each identical chromatographic solution according to qualification result, obtain each sterling.
Common chromatographic column can be divided into normal pressure post, middle compression leg and high-pressure column according to pressure difference. Normal pressure post is with the most use in practice, is usually the chromatographic column that oneself adopts as required different materials, the separation and purification of loading different fillers and come realize target material. Glass chromatography column is especially conventional again, often comprises cylinder, piston, is positioned at also comprising of having the barb for fixing liquid feeding ball of cylinder upper end etc. At present common glass chromatography column exists following not enough: on the one hand, in the filler filling and the filling of separated object quality sample of glass chromatography column, usually adopt different fillers according to different fillers and different material, sample ratio, be beneficial to save filler in reaching optimal separation effect, and filler. the ratio of sample is conventionally with reference to its volume ratio, but at present conventional glass column does not have scale, usually need operating personnel to estimate by rule of thumb or measure with other measurement facilities, bothering very much; On the other hand, in chromatography process, usually needing to be grasped sample is the migration velocity in filler at separating medium, then carry out the speed adjusting of efflux according to migration velocity, be beneficial to save the chromatography time in reaching optimal separation effect, but current chromatographic column does not have scale, usually to the position of certain chromatography colour band marking pen mark in different time points, such way is not accurate enough, the leakage that simultaneously mark of marker pen institute mark may be when adding eluant, eluent and being washed, need mark again, very inconvenient.
In addition, current chromatographic column is in the time of sample introduction, and sample solution directly flows to cylinder from the middle part on chromatographic column top, easily causes filler in cylinder to splash on the inwall of chromatographic column. The filler of using due to chromatography is usually more expensive, and the filler splashing on inwall is not fully utilized in chromatography, affects chromatography effect.
Summary of the invention
For addressing the above problem, the invention provides a kind of chromatographic column for protein purification, adopt following technical scheme:
For a chromatographic column for protein purification, comprise column cap and cylinder, described column cap, is characterized in that for being threaded with cylinder, the top of described column cap is provided with the through hole passing through for plastic tube; Described cylinder adopts transparent plastic material, on described cylinder along cylinder axis to being provided with uniform scale; The bottom that described plastic tube stretches in cylinder connects a four-way pipe, and other three ends of described four-way pipe connect respectively a silica gel hose, and the other end of described silica gel hose contacts with the inwall of cylinder; The bottom of described cylinder is conical outlet; The top of described conical outlet arranges sieve plate, and the aperture of described sieve plate is 0.1 ~ 0.45 μ m.
Preferably, the junction of described column cap and cylinder is provided with sealing ring, and described sealing ring is silica gel material.
The present invention has following beneficial effect:
1. chromatographic column provided by the invention, its cylinder adopts transparent plastic material, and uniform scale is set on cylinder, so arrange, be conducive to easily and accurately hold packing samples ratio in the time of the filler filling of chromatographic column and sample loading, by observing the scale position of certain colour band at different time points place, thus the migration rate of the separated material of convenient grasp in chromatographic column, make whole chromatography process more convenient, improve separative efficiency and whole operating efficiency.
2. chromatographic column provided by the invention, bottom in plastic tube stretches into cylinder connects a four-way pipe, other three ends of described four-way pipe connect respectively a silica gel hose, the other end of described silica gel hose contacts with the inwall of cylinder, so arrange, when being conducive to buffer solution, sample liquid, eluent and entering from cylinder top, to flow down along the sidewall of cylinder, can not cause splashing of the interior liquid of cylinder, cause that filler splashes on the sidewall of cylinder, affect packed height, thereby affect the purification effect of albumen.
3. chromatographic column provided by the invention, top at conical outlet arranges sieve plate, and the aperture that limits sieve plate is 0.1 ~ 0.45 μ m, filler so so just can reach protein purification time not can along with buffer solution, sample liquid, eluent together, run off from conical outlet, but can not affect buffer solution, sample liquid and the eluent outflow effect from conical outlet.
4. chromatographic column provided by the invention, is provided with sealing ring in the junction of column cap and cylinder, is convenient to the airtight of column cap and cylinder, avoids buffer solution, sample liquid, eluent to flow out from both gaps, causes waste.
5. chromatographic column provided by the invention, simple in structure, easy to operate, and the purification effect of albumen is good.
Brief description of the drawings
Fig. 1 is structural representation of the present invention; Wherein, 1 represents column cap; 2 represent plastic tube; 3 represent conical outlet; 4 represent scale; 5 represent sieve plate; 6 represent silica gel hose; 7 represent cylinder.
Detailed description of the invention
Below in conjunction with drawings and Examples, the invention will be further described. Except following embodiment, the present invention can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of requirement of the present invention.
As shown in Figure 1, a kind of chromatographic column for protein purification, comprises column cap 1 and cylinder 7, and described column cap 1, is characterized in that for being threaded with cylinder 7, and the top of described column cap 1 is provided with the through hole passing through for plastic tube 2; Described cylinder 7 adopts transparent plastic material, on described cylinder 7 along cylinder axis to being provided with uniform scale 4; The bottom that described plastic tube 2 stretches in cylinder 7 connects a four-way pipe, and other three ends of described four-way pipe connect respectively a silica gel hose 6, and the other end of described silica gel hose 6 contacts with the inwall of cylinder 7; The bottom of described cylinder 7 is conical outlet 3; The top of described conical outlet 3 arranges sieve plate 5, and the aperture of described sieve plate 5 is 0.1 ~ 0.45 μ m.
As preferably, be provided with sealing ring in the junction of column cap 1 and cylinder 7 and do not mark out in the drawings, for sealing function, described sealing ring is silica gel material, sealing effectiveness is better.
Claims (1)
1. for a chromatographic column for protein purification, comprise column cap (1) and cylinder (7), described column cap (1), is characterized in that for being threaded with cylinder (7), and the top of described column cap (1) is provided with the through hole passing through for plastic tube (2); Described cylinder (7) adopts transparent plastic material, and described cylinder (7) is upper along cylinder axis to being provided with uniform scale (4); The bottom that described plastic tube (2) stretches in cylinder (7) connects a four-way pipe, and other three ends of described four-way pipe connect respectively a silica gel hose (6), and the other end of described silica gel hose (6) contacts with the inwall of cylinder (7); The bottom of described cylinder (7) is conical outlet (3); The top of described conical outlet (3) arranges sieve plate (5), and the aperture of described sieve plate (5) is 0.1 ~ 0.45 μ m;
The junction of described column cap (1) and cylinder (7) is provided with sealing ring, and described sealing ring is silica gel material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310644051.3A CN103691154B (en) | 2013-12-05 | 2013-12-05 | A kind of chromatographic column for protein purification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310644051.3A CN103691154B (en) | 2013-12-05 | 2013-12-05 | A kind of chromatographic column for protein purification |
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| Publication Number | Publication Date |
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| CN103691154A CN103691154A (en) | 2014-04-02 |
| CN103691154B true CN103691154B (en) | 2016-05-11 |
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| CN201310644051.3A Active CN103691154B (en) | 2013-12-05 | 2013-12-05 | A kind of chromatographic column for protein purification |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6280616B1 (en) * | 1995-02-21 | 2001-08-28 | Pharmacia Biotech Ab | Column for chromatography |
| CN201223741Y (en) * | 2008-07-07 | 2009-04-22 | 江苏汉邦科技有限公司 | Small-sized simulated moving bed chromatography device |
| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN202237384U (en) * | 2011-09-19 | 2012-05-30 | 何慧 | Chromatographic column for laboratory |
| CN202410236U (en) * | 2011-12-28 | 2012-09-05 | 上海师范大学 | Detachable chromatographic column with scales |
| CN203678008U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Chromatographic column used for protein purification |
-
2013
- 2013-12-05 CN CN201310644051.3A patent/CN103691154B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6280616B1 (en) * | 1995-02-21 | 2001-08-28 | Pharmacia Biotech Ab | Column for chromatography |
| CN201223741Y (en) * | 2008-07-07 | 2009-04-22 | 江苏汉邦科技有限公司 | Small-sized simulated moving bed chromatography device |
| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN202237384U (en) * | 2011-09-19 | 2012-05-30 | 何慧 | Chromatographic column for laboratory |
| CN202410236U (en) * | 2011-12-28 | 2012-09-05 | 上海师范大学 | Detachable chromatographic column with scales |
| CN203678008U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Chromatographic column used for protein purification |
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| CN103691154A (en) | 2014-04-02 |
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