CN103691150A - Detachable chromatographic column - Google Patents
Detachable chromatographic column Download PDFInfo
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- CN103691150A CN103691150A CN201310643984.0A CN201310643984A CN103691150A CN 103691150 A CN103691150 A CN 103691150A CN 201310643984 A CN201310643984 A CN 201310643984A CN 103691150 A CN103691150 A CN 103691150A
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Abstract
The invention provides a detachable chromatographic column, belonging to the field of biological purification instruments. According to the chromatographic column provided by the invention, the column body is provided with scales, so that the operating personnel can conveniently control the filler height and feed amount; and the top end of the column body is provided with a U-shaped screen, so that a buffer solution, a sample solution, an eluate and other liquids can conveniently flow down along the wall of the column body, thereby avoiding splashing. The column head and column tail are detachable from the column body; and the chromatographic column can be assembled before chromatography, and can be detached after chromatography, so that the column head, column tail and column body can be respectively cleaned, sterilized and the like. The chromatographic column has the advantages of simple structure and favorable protein purification effect, and is convenient to operate.
Description
Technical field
The invention belongs to biologically pure apparatus, be specifically related to a kind of detachable chromatographic column.
Background technology
At present, the separation and purification of the composition of multiple compounds, natural products and Chinese medicine, is mainly to adopt column chromatography, uses chromatographic column, by column chromatography process, realizes.Traditional column chromatography is mixing cpd to be placed on to the top of glass column post bed, make the eluant, eluent (mobile phase) of mixed solvent composition under Action of Gravity Field, by the vertical glass column of absorbent media material (fixedly phase) is housed, separation obtains each purer product (sterling), its principle is: because each component in mixing cpd is to fixing different with the affinity of mobile phase mutually in chromatographic column, therefore select suitable fixedly phase and mobile phase according to the character of mixing cpd, can reach the separated as far as possible object of each component wherein, separated product is collected into respectively in each receiving flask in pillar outlet, then, identify the chromatographic solution of collecting in each receiving flask, according to qualification result, merge and concentrated each identical chromatographic solution, obtain each sterling.
Common chromatographic column can be divided into normal pressure post, middle compression leg and high-pressure column according to pressure difference.Normal pressure post is with the most use in practice, is usually the chromatographic column that oneself adopts as required different materials, loads the separation and purification that different fillers carrys out realize target material.Glass chromatography column is especially conventional again, often comprises cylinder, piston, is positioned at also comprising of having barb for fixing liquid feeding ball of cylinder upper end etc.At present common glass chromatography column exists following not enough: on the one hand, in the filler filling and the filling of separated object quality sample of glass chromatography column, usually according to different fillers and different material, adopt different fillers, sample ratio, be beneficial to save filler when reaching optimal separation effect, and filler. the ratio of sample is conventionally with reference to its volume ratio, but at present conventional glass column does not have scale, usually need operating personnel to estimate by rule of thumb or measure with other measurement facilities, very trouble; On the other hand, in chromatography process, usually needing to be grasped sample is the migration velocity in filler at separating medium, then according to migration velocity, carry out the speed adjusting of efflux, be beneficial to save the chromatography time when reaching optimal separation effect, but current chromatographic column does not have scale, usually to the position of certain chromatography colour band marking pen mark in different time points, such way is not accurate enough, the leakage that simultaneously mark of marker pen institute mark may be when adding eluant, eluent and being washed, need mark again, very inconvenient.
In addition, current chromatographic column is when sample introduction, and sample solution directly flows to cylinder from the middle part on chromatographic column top, easily causes filler in cylinder to splash on the inwall of chromatographic column.The filler of using due to chromatography is usually more expensive, and the filler splashing on inwall is not fully utilized in chromatography, affects chromatography effect.
Summary of the invention
For addressing the above problem, the invention provides a kind of detachable chromatographic column, adopt following technical scheme:
A detachable chromatographic column, comprises column cap and cylinder, and described column cap, is characterized in that for being threaded with cylinder, and the top of described column cap is provided with the through hole passing through for silica gel hose; Described cylinder adopts transparent plastic material, on described cylinder along cylinder axis to being provided with uniform scale; The top of described cylinder arranges dismountable U-shaped screen cloth, and described U-shaped screen cloth is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth; The bottom of described cylinder is threaded connection with post tail and is connected, and the bottom of described post tail is provided with liquid outlet, and the top of described liquid outlet arranges sieve plate, and the aperture of described sieve plate is 0.2 ~ 0.4 μ m.
Preferably, the junction of the junction of described column cap and cylinder, cylinder and post tail is provided with sealing ring, and described sealing ring is silica gel material.
Preferably, the top of described sieve plate arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder.
The present invention has following beneficial effect:
1. chromatographic column provided by the invention, its cylinder adopts transparent plastic material, and uniform scale is set on cylinder, so arrange, be conducive to easily and accurately hold packing samples ratio when the filler filling of chromatographic column and sample loading, by observing certain colour band in the scale position at different time points place, thus the migration rate of the separated material of convenient grasp in chromatographic column, make whole chromatography process more convenient, improve separative efficiency and whole operating efficiency.
2. chromatographic column provided by the invention, dismountable U-shaped screen cloth is set at the top of cylinder, described U-shaped screen cloth is the horn shape for upper end open, lower end closed, and the sidewall of U-shaped screen cloth contains through hole, so setting, when being conducive to buffer solution, sample liquid, eluent and entering from cylinder top, be that wall along cylinder flows down, can not cause splashing of liquid in cylinder, cause that filler splashes on the wall of cylinder, affect packed height, thereby affect the purification effect of albumen.
3. chromatographic column provided by the invention, in the bottom of cylinder, post tail is set, and in the inside of post tail, the top of liquid outlet arranges sieve plate, and the aperture that limits sieve plate is 0.2 ~ 0.4 μ m, and cellulose acetate film is set on sieve plate as optimal way, filler in the time of so so just can reaching protein purification not can along with buffer solution, sample liquid, eluent together, from conical outlet, run off, but can not affect buffer solution, sample liquid and eluent from the outflow effect of conical outlet; In addition, sieve plate is arranged on post tail, after purifying completes, can opens post tail, take out easily the filler in cylinder.
4. chromatographic column provided by the invention, middling speed qualitative filter paper filter membrane is placed on the top of U-shaped screen cloth, can carry out initial gross separation to sample liquid to be separated, and the larger impurity such as untreated thalline completely of interception size ratio, can also avoid chromatography sample to stop up U-shaped screen cloth.
5. chromatographic column provided by the invention, junction at junction, cylinder and the post tail of column cap and cylinder, is all provided with sealing ring, is convenient to the airtight of column cap and cylinder, cylinder and post tail, avoid buffer solution, sample liquid, eluent to flow out from both gaps, cause waste.
6. chromatographic column provided by the invention, column cap and cylinder, cylinder and post tail all can split, and before chromatography, it are assembled, and after chromatography, can mutually take apart, clean respectively, the processing such as sterilizing.The present invention is simple in structure, easy to operate, and the purification effect of albumen is good.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention; Wherein, 1 represents column cap; 2 represent silica gel hose; 3 represent liquid outlet; 4 represent scale; 5 represent sieve plate; 6 represent U-shaped screen cloth; 7 represent cylinder; 8 represent post tail.
The specific embodiment
Below in conjunction with drawings and Examples, the invention will be further described.Except following embodiment, the present invention can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of requirement of the present invention.
As shown in Figure 1,
A detachable chromatographic column, a kind of detachable chromatographic column, comprises column cap 1 and cylinder 7, and described column cap 1, is characterized in that for being threaded with cylinder 7, and the top of described column cap 1 is provided with the through hole passing through for silica gel hose 2; Described cylinder 7 adopts transparent plastic material, on described cylinder 7 along cylinder axis to being provided with uniform scale 4; The top of described cylinder 7 arranges dismountable U-shaped screen cloth 6, and described U-shaped screen cloth 6 is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth 6 contains through hole; Middling speed qualitative filter paper filter membrane (do not mark in the drawings, it is arranged on the top of screen cloth, but is no more than the horizontal level at cylinder top) is placed on the top of described U-shaped screen cloth 6, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth 6; The bottom of described cylinder 7 is threaded connection with post tail 8 and is connected, and the bottom of described post tail 8 is provided with liquid outlet 3, and the top of described liquid outlet 3 arranges sieve plate 5, and the aperture of described sieve plate 5 is 0.2 ~ 0.4 μ m.
Preferably, the junction of the junction of described column cap 1 and cylinder 7, cylinder 7 and post tail 8 is provided with sealing ring, and (not marking out in the drawings), for sealing function, described sealing ring is silica gel material, sealing effectiveness is better.
Preferably, the top of described sieve plate 5 arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder 7.
Claims (3)
1. a detachable chromatographic column, comprises column cap (1) and cylinder (7), and described column cap (1), is characterized in that for being threaded with cylinder (7), and the top of described column cap (1) is provided with the through hole passing through for silica gel hose (2); Described cylinder (7) adopts transparent plastic material, and described cylinder (7) is upper along cylinder axis to being provided with uniform scale (4); The top of described cylinder (7) arranges dismountable U-shaped screen cloth (6), and described U-shaped screen cloth (6) is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth (6) contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth (6), and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth (6); The bottom of described cylinder (7) is threaded connection with post tail (8) and is connected, and the bottom of described post tail (8) is provided with liquid outlet (3), and the top of described liquid outlet (3) arranges sieve plate (5), and the aperture of described sieve plate (5) is 0.2 ~ 0.4 μ m.
2. detachable chromatographic column according to claim 1, is characterized in that, the junction of the junction of described column cap (1) and cylinder (7), cylinder (7) and post tail (8) is provided with sealing ring, and described sealing ring is silica gel material.
3. detachable chromatographic column according to claim 1, is characterized in that, the top of described sieve plate (5) arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310643984.0A CN103691150B (en) | 2013-12-05 | 2013-12-05 | A kind of detachable chromatographic column |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310643984.0A CN103691150B (en) | 2013-12-05 | 2013-12-05 | A kind of detachable chromatographic column |
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| Publication Number | Publication Date |
|---|---|
| CN103691150A true CN103691150A (en) | 2014-04-02 |
| CN103691150B CN103691150B (en) | 2016-04-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310643984.0A Active CN103691150B (en) | 2013-12-05 | 2013-12-05 | A kind of detachable chromatographic column |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105181922A (en) * | 2015-09-18 | 2015-12-23 | 上海昂林科学仪器有限公司 | Device for measuring animal and plant oil |
| CN106267896A (en) * | 2015-06-03 | 2017-01-04 | 中美华世通生物医药科技（武汉）有限公司 | Isolated and purified device and isolation and purification method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN201410310Y (en) * | 2009-03-17 | 2010-02-24 | 温超 | Chromatographic column with scales |
| CN202700138U (en) * | 2012-06-19 | 2013-01-30 | 石家庄诚志永华显示材料有限公司 | Split-type chromatographic column device |
| US8506804B1 (en) * | 2004-05-10 | 2013-08-13 | Scientific Plastic Products, Inc. | Flash chromatography cartridge |
| CN203678013U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Splittable chromatographic column |
-
2013
- 2013-12-05 CN CN201310643984.0A patent/CN103691150B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8506804B1 (en) * | 2004-05-10 | 2013-08-13 | Scientific Plastic Products, Inc. | Flash chromatography cartridge |
| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN201410310Y (en) * | 2009-03-17 | 2010-02-24 | 温超 | Chromatographic column with scales |
| CN202700138U (en) * | 2012-06-19 | 2013-01-30 | 石家庄诚志永华显示材料有限公司 | Split-type chromatographic column device |
| CN203678013U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Splittable chromatographic column |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106267896A (en) * | 2015-06-03 | 2017-01-04 | 中美华世通生物医药科技（武汉）有限公司 | Isolated and purified device and isolation and purification method |
| CN105181922A (en) * | 2015-09-18 | 2015-12-23 | 上海昂林科学仪器有限公司 | Device for measuring animal and plant oil |
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| Publication number | Publication date |
|---|---|
| CN103691150B (en) | 2016-04-06 |
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Denomination of invention: Detachable chromatographic column Effective date of registration: 20190425 Granted publication date: 20160406 Pledgee: Chengdu technical transformation incubator management Co., Ltd. Pledgor: Chengdu Yatu Biotechnology Co., Ltd. Registration number: 2019510000049 |
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Date of cancellation: 20200708 Granted publication date: 20160406 Pledgee: Chengdu technical transformation incubator management Co.,Ltd. Pledgor: YACHT BIOTECHNOLOGY Co. Registration number: 2019510000049 |
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