CN103240136B

CN103240136B - Capillary dispenser

Info

Publication number: CN103240136B
Application number: CN201310042248.XA
Authority: CN
Inventors: 阿德里安·西默斯
Original assignee: Cybio AG
Current assignee: Analytik Jena AG
Priority date: 2012-02-01
Filing date: 2013-02-01
Publication date: 2016-06-22
Anticipated expiration: 2033-02-01
Also published as: EP2623203B1; DE102012100836B3; EP2623203A1; CN103240136A

Abstract

The present invention relates to a kind of Capillary dispenser, it has at least one piston cylinder units (17), this piston cylinder units is made up of the cylinder assembly (16) with inside cylinder (1.1) and the piston component (15) with piston (5), they are collectively arranged on axis (A), wherein, inside cylinder (1.1) is at least connected with pneumatic mode with capillary tube (8), and the piston (5) being embodied as the unilateral hollow piston closed of energy can move between upper end position and the lower end position of piston component (15) along axis (A) in inside cylinder (1.1)。

Description

Capillary dispenser

Technical field

The present invention relates to a kind of Capillary dispenser。

Background technology

Being disclosed a kind of dispenser for dispensing micro volume by patent documentation GB2368640B, it utilizes these capillary tubies to be absorbed the liquid wanting dispensing by capillary effect equipped with following capillary tube。It is stored in the liquid in capillary tube for dispensing, an end of capillary tube produces overvoltage。This can individually but jointly carry out preferably on all capillary tubies, its mode is to be connected with common pressure chamber by capillary tube。

Capillary tube that dispenser described herein is opened by multiple both sides, compression air feeder, constituting for switching compressed-air actuated valve and pressure chamber, this pressure chamber is by the upper-part with compressed air inlet and keeps the lower component of capillary tube to constitute。Upper-part and the lower component of pressure chamber seal relative to each other, thus applying make compression air be uniformly distributed on capillary tube in compressed-air actuated situation and discharged by the liquid in capillary tube at pressure chamber。

Major downside is that of this Capillary dispenser, capillary tube empties in parallel through common pressure supply unit。In order to reliably all capillary tubies are emptied completely, it is necessary at high pressure operation, act on and compress air can be gushed by these capillary tubies emptied with relatively low resistance because the capillary tube emptied plays " short circuit "。Typical in the case operating pressure is 15-25 pound per square inch (psi) or 1.0-1.7 bar。

The air of overflow flows out capillary tube with the speed of every number of seconds rice in the case, and the volume of air discharged depends on the size of pressure chamber, the size of pressure applied and the quantity of capillary tube and is probably several milliliters。Practical situation significantly limit the utilization of this technology。

It is extremely difficult or at all impossible for being released to by discrete drop on the such as slide glass of " smooth " surface, because the air of overflow makes droplet distribution and is distributed on this face。Therefore, only can also consider to release in the recess (groove) of so-called microtitration plate。But also having following restriction at this, i.e. so can only be released in dead slot, spill because can be formed when the air of overflow enters and has been filled with in the groove of liquid, this causes so-called crosstalk between each groove, also referred to as cross-contamination。

Therefore, this Capillary dispenser designs in releasing liquid to dead slot purely。But the problem not thus being fully solved cross-contamination。Capillary tube will not be emptied completely at once。When liquid is discharged, liquid film remains on inside capillary tube, and this liquid film moves slowly toward capillary end due to the air of gravity and overflow。At this, due to the high flowing velocity of air, this liquid film can be atomized into aerosol。This aerosol is due to the activating substance comprising high concentration or utilizes enough sensitive measurement system can also be detected as cross-contamination。

Known so-called air cushion pipettor (Luftpolsterpipetten) in the field of liquid processing system, it can absorb liquid in connected pipettor tip when using piston cylinder units and can release liquid from this pipettor tip。

Pipettor tip is attached on the opening in the side of inside cylinder in the case。Piston inside inside cylinder can arrange and seal relative to this inside cylinder in the way of moving on common axis。In inside cylinder Promotion From Within or reduce the free volume being connected with pipettor tip reducing or increasing inside cylinder by piston, thus can pass through pipettor tip and correspondingly absorb with stereomutation or release liquid。

If instead of pipettor tip, capillary tube is installed, then owing to the free volume of inside cylinder is relatively larger relative to the volume of capillary tube, so capillary tube is owing to capillary effect is independently filled when being immersed in liquid。Then need not promote to will pass through capillary attraction liquid in inside cylinder by piston。

But, the emptying of capillary tube carries out according to the principle of air pressure, and its mode is to reduce cylinder volume and thus being extruded by air therein freely by capillary tube by piston reduction in inside cylinder。

When arranging a piston for each single capillary tube, air pressure can very finely dosage。This is capable of on free capillary end to form drop, and this drop can be moved liquid by contacting with such as slide glass targetedly。

If the acceleration of piston and the speed that can reach are sufficiently large, then can also contactlessly release the drop/liquid beam of i.e. free flight。Pressure needed for single in the case capillary tube is less than the pressure when making all capillary tube common discharge, because this pressure need not as designed due to the such ultra-specification needed for possible " short circuit " (being set forth in the description of prior art such as this short circuit)。

By the volume freely of the inside cylinder of piston pressure and therefore from capillary tube, therefore the air of overflow, in an experiment less than 50 μ l, and is one of 1 to two/20th percentage during the Capillary dispenser utilizing all capillary tubies and pressure chamber to connect。

At less pressure with thus given less volume of air can not only realize being dispensed on smooth surface while less emitting rate, and can also be dispensed into contained liquid container, in the groove of microtitration plate。Aerosol is formed and thus also largely avoid。

Due to the advantage being previously mentioned, those skilled in the art seemingly can be promoted to go to use the piston cylinder units being connected respectively with capillary tube。But with capillary tube rather than be connected the shortcoming brought with pipettor and cause that those skilled in the art deviate this design。

After releasing, piston must be made again to reset in initial position before again releasing when avoiding less desirable liquid to absorb every time。

Summary of the invention

The task of the present invention is to find a kind of Capillary dispenser, and it has at least one piston cylinder units, and reliably avoids by mistake absorbing liquid by the measure in structure in this Capillary dispenser。

Advantageously, Capillary dispenser is wanted only to revise with the change in small structure, in order to even if alternatively for contactlessly sending in liquid or sending and optimize Capillary dispenser when contacting capillary tube。

Advantageously, capillary tube want can with high repetitive rate quick-replaceable。This task utilizes the feature of claim 1 to solve for Capillary dispenser。The enforcement having the advantage that is described in the dependent claims。

Accompanying drawing explanation

It is set forth in the present invention by accompanying drawing hereinafter with reference to embodiment。For this:

Fig. 1 a illustrates the three-dimensional view of the assembly of Capillary dispenser；

Fig. 1 b illustrates the detailed view of the assembly according to Fig. 1 a；

Fig. 2 illustrates the cross section diagram of the assembly according to Fig. 1 a；

Fig. 3 a illustrates the piston cylinder units 17 in the upper end position being in piston component 15 with the first form of implementation carrying out piston seal by piston seal plate 6；

Fig. 3 b illustrates the piston cylinder units according to Fig. 3 a in the lower end position being in piston component 15；

Fig. 4 a illustrates the Local map of the piston cylinder units 17 in the upper end position being in piston component 15 with the second form of implementation carrying out piston seal by spheroid 12；

Fig. 4 b illustrates the Local map of the piston cylinder units 17 in the lower end position being in piston component 15 with the second form of implementation carrying out piston seal by spheroid 12；

Fig. 5 illustrates the piston cylinder units 17 with valve 14。

Detailed description of the invention

Substantially, can equipped with only one capillary tube, multiple capillary tube or multiple capillary tube arranged in a matrix of arranging of being in line according to the Capillary dispenser of the present invention。Capillary tube can be associated with piston cylinder units respectively, or multiple capillary tube is connected with common cylinder and is thus associated with common piston cylinder units 17。

Because Capillary dispenser substantially disposes as follows, namely。Make its dispensing on gravitational direction, thus in ensuing elaboration as " on " or the statement of D score be clear and definite。These statements also should so be understood when Capillary dispenser does not act as land used operation in vertical direction, is applied especially to the Capillary dispenser with only one capillary tube for this。

Next when describing the structurally and functionally mode of Capillary dispenser by piston cylinder units 17, then this explanation is respectively suitable for the piston cylinder units of all of Capillary dispenser。

Substantially, having at least one piston cylinder units 17 according to the Capillary dispenser of the present invention, it is made up of the cylinder assembly 16 with inside cylinder 1.1 and the piston component 15 with piston 5。Inside cylinder 1.1 and piston 5 are arranged on the axis a coaxially with each other, and wherein, piston 5 seals relative to inside cylinder 1.1, can move a stroke along axis A in this inside cylinder。Substantially, it is possible to manually guide this motion, but especially when Capillary dispenser has large number of capillary tube 8, it is possible to control this motion with electric motor-driving way, for this Capillary dispenser, there is driving device and control device。

For the present invention it is essential that piston 5 is following hollow piston, this hollow piston is opened when it is initially towards the upper end position motion of piston component 15 and then keeps both sides to open in the length of stroke。

During moving towards the lower end position of piston component 15, piston 5 coverlet side seal when starting closes and keeps one side to close in the whole length of stroke。The upper end position of piston component 15 and lower end position restriction stroke。Inside cylinder 1.1 is connected with pneumatic mode with at least one capillary tube 8。

Because piston 5 during it moves towards the upper end position of piston component 15 be open and piston cylinder units 17 be thus open systems, so pressure ratio on the end being connected with inside cylinder 1.1 of at least one capillary tube 8 is constant during piston 5 moves towards the upper end position of piston component 15。Pressure ratio on capillary tube 8 is not thus affected by this motion。It is to say, capillary tube 8 can have been filled with, as long as or simultaneously it contacts with liquid with its other end (being next called free end), it is possible to only filled by capillary effect。

Piston 5 towards piston component 15 lower end position move during, this piston be Guan Bi and piston cylinder units 17 be thus closed-system。Air in piston cylinder units 17 is compressed, and thus during piston 5 moves towards the lower end position of piston component 15, pressure on the end being connected with inside cylinder 1.1 of at least one capillary tube 8 improves constantly。Air is gushed by least one capillary tube 8, and the liquid being thus located therein is blown。

Because piston 5 must be opened (aerofluxus) after capillary tube 8 empties every time, that is, piston component 15 must on the direction of the upper end position of piston component 15 at least one fraction of stroke of motion, so what have the advantage that is, when piston 5 and inside cylinder 1.1 determine specification as follows in its length and its cross section, that is, stroke is made just to be enough to empty capillary tube 8。So, empty and aerofluxus makes full use of complete stroke and inside cylinder 1.1 and piston 5 can determine specification as small as possible。

It will be apparent to those skilled in the art that and determine that specification can also carry out as follows, i.e. make a stroke be enough to more than 8 emptying of capillary tube。In order to by capillary tube 8 aerofluxus and refill, then piston component 15 only promotes a bit on the direction of the upper end position of piston component 15, so that capillary tube 8 is opened。

Because the pressure in inside cylinder 1.1 not raises suddenly and is also only smaller, so liquid is likely in the container being released to existing liquid, without thus there is so-called crosstalk, as described in the explanation to prior art。When being provided with valve 14 in the connection between inside cylinder 1.1 and capillary tube 8, it is possible that pulsed is released。

Substantially, where closed piston 5 is unessential for the model of action of piston cylinder units 17。This can on the bottom that piston 5 guides in inside cylinder 1.1 in principle, any position in the upper end of piston 5 or carry out preferably on the upper end of piston 5。

Close preferably by piston sealing device。The piston 5 or direct end face built with building advantageously rounding on upper end rests against on piston sealing device, otherwise the mode that arrange either directly through the end that will be around piston 5, that rest against piston seal relative to the piston head 5.1 of its peripheral sealing and open that seals of piston 5 carries out。The scheme finally mentioned has the advantage that, i.e. by piston head 5.1 being determined, size can provide broader contact surface。Piston sealing device preferably may be embodied as piston seal plate 6, and this piston seal plate is made up of elastomeric material such as synthetic rubber。Then the sealing of all pistons 5 advantageously utilizes identical piston seal plate 6 to carry out。Instead, it is possible to use each cone or spheroid 12 are as piston sealing device。These cones or spheroid 12 such as can be made up of glass, pottery or also, it is preferred that can be made up of elastomeric material。By piston head 5.1, piston 5 is suspended in piston component 15。

Next several embodiment distinguished by the form of implementation in the different structure of various different parts such as head sealing member, piston head 5.1, valve 14 and capillary tube keepers 7 is described。The different form of implementation energy combinations with one another of different parts, even if here not mentioning each that be likely to combination in detail。

A kind of embodiment having the advantage that meeting the requirement to the precision and rapidity releasing minimum volume the most is the capillary dispenser with a large amount of capillary tubies 8, the groove of the microtiter plates of these capillary tubies and commercial standard (CS) is respectively disposed in matrix each other, and has a piston cylinder units 17 for each capillary tube 8。

Such capillary dispenser is set forth in the first embodiment with reference to Fig. 1-3。

The capillary dispenser of capillary tube 8 arranged in rectangular that has shown in Fig. 1 a has loading plate 1, pressing plate 2 and piston holding plate 3 and the storehouse casket 9 resting on loading plate 1 indirectly by sealing gasket 10, in this storehouse casket capillary tube keeper 7 together with capillary tube 8 with hang, rest on sealing gasket 10 to force closure。

Each capillary tube 8 is provided with a piston cylinder units 17, and piston cylinder units 17 is respectively arranged on the axis a。Exemplarily depict an axis A in axis A in fig 1 a。In order to it can be seen that piston holding plate 3, be shown without the piston seal plate 6 hiding piston holding plate 3 and the baffle plate 4 being arranged on piston seal plate 6 in fig 1 a, they are associated with piston cylinder units 17 equally。

Portion of leaving a blank shown in loading plate 1 is arranged for (assembly needed for automatic operating is as controlled assembly) motor and actuating device。Loading plate 1 is concurrently formed at the downside of this unshowned housing。

The different embodiments of piston cylinder units 17 can be seen as cross section diagram in Fig. 2 to Fig. 5。

Fig. 3 a and Fig. 3 b illustrate the first form of implementation of piston cylinder units 17, wherein, has illustrated the piston component 15 being in upper end position in fig. 3 a and illustrate the piston component 15 that is in lower end position in fig 3b。

Piston cylinder units 17 mainly includes inside cylinder 1.1 and piston 5, and this piston 5 is concentrically arranged in this inside cylinder on common axis A with inside cylinder 1.1。Being provided with capillary tube 8 equally on the axis a, it is connected with pneumatic mode with inside cylinder 1.1。Piston 5 is the hollow piston that both sides are opened, and this hollow piston can unilateral be closed。

The inside cylinder 1.1 of piston cylinder units 17 is formed by the reach through hole in loading plate 1。Reach through hole can be the hole of classification as shown, comprising: have the section of larger internal diameter, piston 5 runs in this section；With the section with small diameter, in order to form passage, the diameter of this passage is more than the diameter of capillary tube 8, but restraints the diameter of 7.2 less than the tip built on capillary tube keeper 7。The length of the section that piston 5 runs wherein depends on stroke and piston head 5.1 had axial gap in the recess 3.1 of piston plate 3 and selects, and the length of this section is combined with the diameter of piston 5 to be determined and maximum send volume。The grid that capillary tube 8 is arranged therein is more little, inevitably must determine more little by the diameter of inside cylinder 1.1 and the length of therefore piston 5 runs wherein section must be more big。

In order to be sealed relative to inside cylinder 1.1 by piston 5, between pressing plate 2 and loading plate 1, it is provided with the seal sleeve 11 that piston 5 is sealed encirclement。

Have and be collectively forming and the cylinder assembly 16 of capillary tube 8 equal number existed with the seal sleeve 11 with the pressing plate 2 and respective numbers of the reach through hole of inside cylinder 1.1 identical set to arrange the loading plate 1 of the inside cylinder 1.1 built in rectangular, to have。

The piston 5 of piston cylinder units 17 is following hollow piston, installs or be built with piston head 5.1 on the upper end of this hollow piston。Piston head 5.1 surrounds the upper end of piston 5 as the axle collar, and has two end face 5.1.1 and 5.1.2 being perpendicular to axis A distribution。Piston head 5.1 can be lain in by lower surface 5.1.1 in the recess 3.1 of piston holding plate 3, and this recess is more deeper than the height of piston head 5.1。Thus, piston head 5.1 has axial gap on the direction of axis A, and this gap is equal to the difference between the height and the degree of depth of recess 3.1 of piston head 5.1。Upper surface 5.1.2 protrudes beyond the upper end of piston 5, and thus upper surface 5.1.2 can rest against on the piston sealing device being embodied as piston seal plate 6。

Substantially, the upper end of piston 5 can also protrude beyond upper surface 5.1.2, and thus the upper end of piston 5 can rest against on piston seal plate 6。

As from Fig. 1 a it is readily apparent that the upside of piston holding plate 3 has a large amount of recess 3.1 with the grid form in rectangular identical with capillary tube 8。The recess 3.1 arranged that is in line respectively is connected to each other by groove 3.2, and these grooves and the piston sealing device kept flat being embodied as piston seal plate 6 are collectively forming air duct。Being provided with baffle plate 4 on piston seal plate 6, this baffle plate is fastened on piston holding plate 3 via releasable indirectly by piston seal plate 6。

Piston holding plate 3 and baffle plate 4 can move relative to fixed loading plate 1 together along axis A, and thus piston 5 can correspondingly promote with the length of stroke and reduce in inside cylinder 1.1。

Piston holding plate 3, piston seal plate 6, baffle plate 4 and there is the piston 5 being separately positioned on piston head 5.1 thereon be collectively forming and the piston component 15 of capillary tube 8 equal number existed。

The capillary tube 8 being associated with piston cylinder units 17 is connected with pneumatic mode with inside cylinder 1.1。Substantially at this it is contemplated that following any kind of connection, this connection is the connection sealed relative to surrounding。

In order to be connected with Capillary dispenser by the capillary tube arranged in rectangular 8, capillary tube 8 introduces in the storehouse casket 9 with corresponding Grill space each other, and this storehouse casket 9 is rested on loading plate 1 in locking manner by sealing gasket 10 indirect force。It follows that term " storehouse casket " 9 to represent following surface plate, this surface plate is provided with capillary tube 8, does not rely on how these capillary tubies are maintained in plate and they whether releasably or not attachment ground or non-cohesive be maintained in plate in other words。

At ratio in the embodiment less having the advantage that, capillary tube 8 can such as enter in storehouse casket 9 securely。It is that capillary tube 8 that is impaired, that break or that block can not be replaced, and this needs to change whole storehouse casket 9 if desired in this shortcoming。

Instead, capillary tube 8 can also be inlaid in bar (being such as made up of plastics) individually, and by be arranged on this bar releasable threaded, be clamped and connected or socket connection is releasably secured in storehouse casket 9。Have the obvious benefit that for above-mentioned solution, replace individually and simply the capillary tube 8 with bar, be connected to this bar and storehouse casket 9 positive and/or force closure。Shortcoming is, this connection at random can not frequently get loose and not wear and tear, and the suction-combining force for reinforcing this connection neither allow excessive do not allow yet too small, in order to do not damage bar on the one hand and ensure firmly to coordinate on the other hand。

The solution having the advantage that for being embedded in capillary tube 8 proposes to introduce in capillary tube keeper 7 respectively by these capillary tubies, and this capillary tube keeper is implemented and shown in Fig. 3 a and 3b as pipettor tip。Capillary tube keeper is made up of at this tip portion 7.1 most advanced and sophisticated bundle 7.2 on end rearward on dispensing direction the tip portion 7.1 of tubulose and building, and the rear end side and front that have towards tip portion 7.1 are restrainted in this tip。

Capillary tube keeper 7 can be inserted in the hole for this setting in storehouse casket 9 with its tip portion 7.1, until the rear end side of most advanced and sophisticated bundle 7.2 rests on storehouse casket 9 and capillary tube keeper 7 is suspended in storehouse casket 9 with smallest radial gap。In order to change capillary tube keeper 7 and thus change capillary tube 8, need not be got loose connection, but only by promoting without resistance, capillary tube keeper 7 is pulled out from storehouse casket 9, therefore change the mechanical load of the capillary tube keeper 7 that will not cause and thus without the abrasion caused owing to replacing causes。

Capillary tube 8 is attached on the leading section of capillary tube keeper 7, and at least slightly protruding exceedes this leading section。Capillary tube can such as be pasted or be pressed into。Capillary tube keeper 7 can also advantageously manufacture the overall moulding with the capillary tube 8 as insert。

Storehouse casket 9 can position and be attached on loading plate 1, more precisely, make most advanced and sophisticated bundle 7.2 front force closure rest against on sealing gasket 10。

Capillary tube 8 is preferably made up of glass but it also may be made up of other materials such as plastics or pottery, wherein, it is necessary to assure, the capillary rise height of the liquid of dispensing to be enough to be filled up completely with capillary tube 8。Capillary rise height h is calculated by equation below:

h = \frac{2 γ c o s Θ}{ρ g R}

At this, γ is the surface tension of liquid, and θ is angle of wetting, and ρ is the density of liquid, the inside radius that g is acceleration of gravity and R is capillary tube 8。

Capillary tube 8 preferably has hydrophobic external coating, in order to suppress liquid to climb on the outside of capillary tube 8 when stoping liquid adhesive when mentioning from liquid as far as possible and form drop on capillary end。What have the advantage that especially is the coating of fluoropolymer composition。

Advantageously, capillary tube 8 or its free end thin-walled as far as possible ground are implemented, in order to make the end face on capillary end minimize and thus make liquid adhesive capacity on end face minimize。

Next combine shown capillary tube 8 by the function such as the piston cylinder units 17 as shown in Fig. 3 a and Fig. 3 b and set forth the working method of the Capillary dispenser according to the present invention。

When dispensing process starts, piston component 15 is in upper end position, as shown in fig. 3 a。Piston head 5.1 is on the bottom of the recess 3.1 in piston holding plate 3。In piston 5, its lower end is in inside cylinder 1.1, and its upper end is connected with air by air duct, and therefore in inside cylinder 1.1, ambient pressure is in leading。

Because capillary tube 8 independently can be filled by its free end of submergence by its capillarity, as long as the other end is opened and applies and ambient pressure essentially identical on free end, so capillary tube 8 has been filled with or is filled now by being immersed in liquid。Preferably will be filled with the container of liquid to promote towards capillary tube 8 for this。

For dispensing, having must be set up pressure in inside cylinder 1.1, in order to drain from capillary tube 8, precondition is, inside cylinder 1.1 is closed and free volume in inside cylinder 1.1 is then reduced。The unilateral closing indirectly by piston 5 of closing of inside cylinder 1.1 carries out, and its mode is to make piston component 15 reduce。After the path of the axial gap equal to piston head 5.1, piston head 5.1 is pressed against on baffle plate 4 indirectly by piston seal plate 6 and is closed。Piston cylinder units 17 thus relative to surrounding seal, and when piston component 15 and thus piston 5 set up for by liquid pressure of dispensing from capillary tube 8 when reducing further, until this pressure is enough to drain。To this referring to Fig. 3 b。The lower end position of piston component 15 gives on pressing plate 2 by being rested on by piston holding plate 3, but can be limited by the backstop of additional existence too。

After releasing liquid completely, piston 5 is brought in its upper end position by the lifting of piston component 15 again, and wherein, piston 5 is again turned on, so that again occur ambient pressure in inside cylinder 1.1。At this or then, liquid can be reuptaked by capillary tube 8, wherein, absorb not by suction but only caused by capillary effect。Described working method is synchronously performed for all piston cylinder units 17 of Capillary dispenser。

In a second embodiment, Capillary dispenser to have the capillary tube 8 for piston cylinder units 17 integral multiple, being once described in the first embodiment such as them。

In the case, the quantity capillary tube 8 corresponding to piston cylinder units 17 several times it is connected to each inside cylinder 1.1。Along with the increase of the quantity of the capillary tube 8 emptied by piston cylinder units 17, inside cylinder 1.1 must be set up bigger pressure。

Replace the piston seal plate 6 as the piston sealing device for all piston cylinder units 17, it is also possible to using such as spheroid 12 to be sealed by piston 5 for each piston cylinder units 17, this spheroid is placed in recess 13.1 in additional accessory plate 13。

Illustrate the intercept with the piston component 15 as the spheroid 12 in the piston sealing device upper end position at piston component 15 and lower end position in figs 4 a and 4b。

Identical with embodiment described above, in the upper end position of piston component 15, piston head 5.1 rests against on its bottom in recess 3.1, and piston 5 is opened。

In the lower end position of piston component 15, the upper end of piston 5 directly rests against on spheroid 12 and piston 15 is to close。Spheroid 12 can be manufactured by synthetic rubber or be made by having the pottery of high-precision surface, glass or metal。When stiff spheroid 12, the upper end of piston 5 is formed with the end face with the radius of curvature equal with radius of sphericity。Spheroid 12 is placed on as in the recess 13.1 of this setting respectively on each piston 5 in accessory plate 13。Groove 3.2 it is also equipped with in the present embodiment in piston holding plate 3。These grooves correspondingly can also introduce in the adjacent side of accessory plate 13。

Another embodiment to be set forth with reference to Fig. 5。

For contactlessly dispensing, as set forth in the description of prior art, it is necessary to high piston acceleration/piston speed, in order to set up the pressure needed for dispensing rapidly accordingly。This can be realized by big piston diameter。If but be associated with only one capillary tube 8 for this each inside cylinder 1.1, then structure space is limited by the grid of capillary tube 8, grid (the 96 formula plates=9mm of the groove of this grid and Target Board；384 formula plates=4.5mm；1536 formula plates=2.25mm) coupling。

In order to deal with when there is no high acceleration/speed, it is also possible to valve 14 is placed on the bottom of inside cylinder 1.1。In inside cylinder 1.1, the pressure needed for dispensing can be set up by piston 5 by valve 14, and then discharge as pressure pulse。For this, it may be considered that Minitype electromagnetic valve, and passive spheres check-valves and the so-called cross valve being made up of synthetic rubber or pressure bell (Druckbills)。When passive valves, problem is, pressure is generally not intended as pulse release by them, but opens lentamente when reaching threshold value。In order to utilize passive valves also can produce pressure pulse, propose to determine as follows the specification of valve 14, i.e. make also to be always up Guan Bi when its pressure that maximum energy produces in inside cylinder 1.1, and just mechanically opened by the applying of piston 5, so that pressure can release suddenly。

Reference numerals list

1 loading plate

1.1 inside cylinders

2 pressing plates

3 piston holding plates

3.1 recesses

3.2 grooves

4 baffle plates

5 pistons

5.1 piston heads

5.1.1 the lower surface of piston head

5.1.2 the upper surface of piston head

6 piston seal plates

7 capillary tube keepers

7.1 tip portions

7.2 most advanced and sophisticated bundles

8 capillary tubies

9 storehouse caskets

10 sealing gaskets

11 seal sleeves

12 spheroids

13 accessory plates

13.1 recess

14 valves

15 piston components

16 cylinder assemblies

17 piston cylinder units

A axis

Claims

1. Capillary dispenser, it has at least one piston cylinder units (17), described piston cylinder units (17) is made up of the cylinder assembly (16) with inside cylinder (1.1) and the piston component (15) with piston (5), they are collectively arranged on axis (A), wherein, described inside cylinder (1.1) is at least connected with pneumatic mode with capillary tube (8), wherein, described piston (5) is embodied as the hollow piston opened both sides and can move between upper end position and the lower end position of described piston component (15) along described axis (A) in described inside cylinder (1.1), and wherein, described piston (5) can unilateral be closed, to close unilateral for described piston (5) during moving towards the lower end position of described piston component (15)。

2. Capillary dispenser according to claim 1, it is characterized in that, piston sealing device belongs to described piston component (15), in the lower end position of described piston component (15), force closure ground setting is to described piston sealing device in the upper surface of described piston (5), and thus described piston (5) closing and described piston (5) have axial gap in described piston component (15) inside on the direction of described axis (A)。

3. Capillary dispenser according to claim 1, it is characterized in that, piston sealing device belongs to described piston component (15), and on described piston (5), there is piston head (5.1), described piston head (5.1) protrudes beyond the upper surface of described piston (5) and rests against to described piston sealing device to force closure in the lower end position of described piston component (15), thus described piston (5) closing and described piston (5) have axial gap in described piston component (15) inside on the direction of described axis (A)。

4. the Capillary dispenser according to Claims 2 or 3, it is characterized in that, described piston sealing device is spheroid (12), and the upper surface of described piston (5) is built with the radius of curvature equal with the radius of described spheroid (12)。

5. the Capillary dispenser according to Claims 2 or 3, it is characterised in that described piston sealing device is piston seal plate (6)。

6. Capillary dispenser according to claim 1, it is characterised in that described inside cylinder (1.1) is connected by valve (14) with described capillary tube (8)。

7. Capillary dispenser according to claim 1, it is characterised in that at least one capillary tube (8) press-in capillary tube keeper (7), described capillary tube keeper has the form of pipette tip。

8. Capillary dispenser according to claim 1, it is characterized in that, described Capillary dispenser includes a large amount of capillary tube (8), and each capillary tube (8) is existed to the capillary tube keeper (7) of a pipette tip format, described capillary tube (8) in the way of free end protrusion, be tightly inserted in described capillary tube keeper (7), and described capillary tube keeper (7) is arranged in storehouse casket (9) in a hanging manner。

9. Capillary dispenser according to claim 1, it is characterised in that each inside cylinder (1.1) is connected with multiple capillary tubies (8)。