CN103240136A - Capillary dispenser - Google Patents
Capillary dispenser Download PDFInfo
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- CN103240136A CN103240136A CN201310042248XA CN201310042248A CN103240136A CN 103240136 A CN103240136 A CN 103240136A CN 201310042248X A CN201310042248X A CN 201310042248XA CN 201310042248 A CN201310042248 A CN 201310042248A CN 103240136 A CN103240136 A CN 103240136A
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/022—Capillary pipettes, i.e. having very small bore
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0275—Interchangeable or disposable dispensing tips
- B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
- B01L3/0265—Drop counters; Drop formers using valves to interrupt or meter fluid flow, e.g. using solenoids or metering valves
Abstract
The capillary dispenser has a piston cylinder unit (17) that includes a cylinder assembly (16) with inner cylinder (1-1), and a piston assembly (15) with hollow piston (5). The inner cylinder is connected with a capillary (8). The piston is moved in inner cylinder along the axis (A) between the upper and lower end positions of piston assembly. The piston is closed on one side, during the movement of piston to the lower end position of piston assembly.
Description
Technical field
The present invention relates to a kind of capillary dispenser.
Background technology
Disclose a kind of dispenser for the small volume of dispensing by patent documentation GB23 686 40B, it is equipped with following capillary, utilizes these capillaries to absorb the liquid of wanting dispensing by capillary effect.For dispensing is stored in liquid in the capillary, produce overvoltage in an end capillaceous.This can be individually but preferably jointly carries out on all capillaries, and its mode is that capillary is connected with common balancing gate pit.
The capillary that dispenser described herein is opened by a plurality of both sides, compressed air feedway, be used for the compressed-air actuated valve of switch and the balancing gate pit constitutes, this balancing gate pit is by the upper-part with compressed air inlet and keep lower member capillaceous to constitute.Upper-part and the lower member of balancing gate pit seal relative to each other, compressed air are distributed on the capillary and with the liquid in capillary equably discharge thereby apply in the balancing gate pit.
The major defect of this capillary dispenser is that capillary comes emptying by common pressure supply unit concurrently.In order reliably all capillaries to be emptied completely, need under high pressure, work because the capillary of emptying play " short circuit " effect and compressed air can with relatively low resistance by these the capillary of emptying gush.Typical in the case operating pressure is 15-25 pound per square inch (psi) or 1.0-1.7 bar.
The air of overflow flows out capillary with the speed of per second number meter in the case, and the volume of air of discharging depends on the size of balancing gate pit, size and the quantity capillaceous of institute's applied pressure may be several milliliters.Actual conditions have greatly limited the utilization of this technology.
It is very difficult or impossible at all being released to discrete drop on " smooth " surperficial for example slide glass, because the air of overflow disperses drop and is distributed on this face.Therefore, only can also consider to emit in the recess (groove) at so-called microtiter plate.But at this following restriction is arranged also, that is, can only be released in the dead slot like this, spill because can form when the air of overflow enters in the groove of filling liquid, this causes so-called crosstalking between each groove, is also referred to as cross pollution.
Therefore, this capillary dispenser is purely at emitting liquid in dead slot and design.Yet do not solve the problem of cross pollution thus fully.Capillary can be emptied completely at once.When liquid was discharged, liquid film remained on the capillary inboard, and this liquid film moves towards capillary end lentamente owing to the air of gravity and overflow.At this, because the high flowing velocity of air, this liquid film can be atomized into aerosol.This aerosol is owing to comprise the activating substance of high concentration or utilize enough responsive measuring system also can be detected as cross pollution.
Known so-called air cushion pipettor (Luftpolsterpipetten) in the field of liquid processing system, it can absorb liquid and can emit liquid from this pipettor tip under the situation of using piston cylinder units in connected pipettor tip.
The pipettor tip is attached on the opening in inside cylinder distolateral in the case.Piston is arranged in the mode that can move at common axis in inside cylinder inside and is sealed with respect to this inside cylinder.By piston in the inside cylinder Promotion From Within or reduce reduce or increases inside cylinder with the most advanced and sophisticated free volume that is connected of pipettor, can correspondingly absorb or emit liquid by pipettor tip and stereomutation thus.
If replace the pipettor tip capillary is installed, so because the free volume of inside cylinder is bigger with respect to volume ratio capillaceous, so capillary is owing to independently fill under the situation of capillary effect in being immersed into liquid.So do not need piston in inside cylinder, to promote in order to absorb liquid by capillary.
Yet emptying capillaceous is carried out according to the principle of air pressure, and its mode is to dwindle cylinder volume freely and thus air is wherein extruded by capillary by the reduction of piston in inside cylinder.
When for each single capillary a piston being set, the air pressure can very fine ground dosage.This can be implemented on the free capillary end and form drop, and this drop can be moved liquid targetedly by contacting with for example slide glass.
If the acceleration of piston and the speed that can reach are enough big, so also can contactlessly emit is the drop/liquid beam of free flight.The required pressure of single in the case capillary is less than the pressure when making the common exhaust of all capillaries because this pressure needn't as since the required such ultra-specification of possible " short circuit " (being set forth like that in the description of prior art as this short circuit) design.
By the air of the volume freely of the inside cylinder of piston pressure and therefore overflow from capillary in experiment less than 50 μ l, and therefore be one of 1/20th to two percentage when utilizing all capillaries with capillary dispenser that a balancing gate pit is connected.
Less pressure and thus less emit speed in given less volume of air not only can realize dispensing to smooth surface, and can dispensing in the container that contains liquid, groove as microtiter plate.Aerosol forms and has also avoided to a great extent thus.
Because the advantage of mentioning can impel those skilled in the art to go to use the piston cylinder units that is connected with capillary respectively seemingly.Yet being connected the shortcoming of bringing with capillary rather than with pipettor causes those skilled in the art to depart from this design.
After emitting, under the situation that the liquid of avoiding not expecting absorbs, piston was reset in the initial position again before emitting again at every turn.
Summary of the invention
Task of the present invention is to find a kind of capillary dispenser, and it has at least one piston cylinder units, and avoids by mistake absorbing liquid reliably by structural measure in this capillary dispenser.
Have advantage ground, the capillary dispenser is wanted and can only be revised with small structural change, even so that alternatively at contactlessly sending in liquid or sending under contact situation capillaceous optimized the capillary dispenser.
Have advantage ground, capillary is wanted and can be changed fast with high repetitive rate.This task utilizes the feature of claim 1 to solve for the capillary dispenser.Enforcement with advantage is described in the dependent claims.
Description of drawings
At length set forth the present invention hereinafter with reference to embodiment by accompanying drawing.For this reason:
Fig. 1 a illustrates the three-dimensional view of the assembly of capillary dispenser;
Fig. 1 b illustrates the detailed view according to the assembly of Fig. 1 a;
Fig. 2 illustrates the cross section diagram according to the assembly of Fig. 1 a;
Fig. 3 a illustrates to have by piston seal plate 6 and carries out piston cylinder units 17 in the upper end position that is in piston component 15 of first form of implementation of piston seal;
Fig. 3 b illustrates the piston cylinder units according to Fig. 3 a in the lower end position that is in piston component 15;
Fig. 4 a illustrates has the part figure that carries out the piston cylinder units 17 in the upper end position that is in piston component 15 of second form of implementation of piston seal by spheroid 12;
Fig. 4 b illustrates has the part figure that carries out the piston cylinder units 17 in the lower end position that is in piston component 15 of second form of implementation of piston seal by spheroid 12;
Fig. 5 illustrates the piston cylinder units 17 with valve 14.
The specific embodiment
Basically, capillary dispenser according to the present invention only can be equipped with a capillary, a plurality of capillary or a plurality of capillary of arranging that is in line and arranges in matrix.Capillary can be attached troops to a unit in piston cylinder units respectively, or a plurality of capillary is connected with common cylinder and attaches troops to a unit thus in common piston cylinder units 17.
Because the capillary dispenser is settled basically as follows, namely.Make its dispensing on gravitational direction, thus in ensuing elaboration as " on " or the statement of D score be clear and definite.These statements also should so be understood when the capillary dispenser is not operated in vertical direction with working, especially are applied to have only capillary dispenser capillaceous for this reason.
When next describing the structure of capillary dispenser and the mode of action by piston cylinder units 17, so this explanation is applicable to the piston cylinder units of all capillary dispensers respectively.
Basically, capillary dispenser according to the present invention has at least one piston cylinder units 17, and it is made of the cylinder assembly 16 with inside cylinder 1.1 and the piston component 15 with piston 5.Inside cylinder 1.1 and piston 5 are arranged on the axis A coaxially with each other, and wherein, piston 5 is with respect to inside cylinder 1.1 sealing, can be along the axis A stroke that moves in this inside cylinder.Basically, can manually guide this motion, but especially when the capillary dispenser has the capillary 8 of larger amt, can control this motion in the Motor Drive mode, the capillary dispenser has drive unit and control device for this reason.
For the present invention importantly, piston 5 is following hollow pistons, and this hollow piston begins to be opened when the upper end position of piston component 15 moves and to open in the length maintenance both sides of stroke then at it.
Towards the lower end position of piston component 15 between moving period, piston 5 coverlet side seal when beginning closes and keeps one-sided sealing in the whole length of stroke.The upper end position of piston component 15 and lower end position restriction stroke.Inside cylinder 1.1 and at least one capillary 8 are connected with pneumatic mode.
Because piston 5 its upper end position towards piston component 15 between moving period be open and piston cylinder units 17 be open system thus, so piston 5 towards the upper end position of piston component 15 between moving period the pressure ratio at least one capillary 8 and end that inside cylinder 1.1 is connected constant.This motion is thus to not influence of the pressure ratio on capillary 8.That is to say that capillary 8 can be filled, or simultaneously as long as it contacts with liquid with its other end (next being called free end), just can only fill by capillary effect.
Towards the lower end position of piston component 15 between moving period, this piston is closed and piston cylinder units 17 is closed-system thus at piston 5.Air in piston cylinder units 17 is compressed, thus piston 5 towards the lower end position of piston component 15 between moving period the pressure at least one capillary 8 and end that inside cylinder 1.1 is connected improve constantly.Air gushes by at least one capillary 8, and the liquid that is positioned at thus wherein is blown.
Because piston 5 must be opened after capillary 8 each emptyings (exhaust), that is to say, piston component 15 must be on the direction of the upper end position of piston component 15 at least one fraction of stroke of motion, so what have advantage is, when piston 5 and inside cylinder 1.1 are determined specification as follows on its length and its cross section, that is, stroke is enough to just with capillary 8 emptyings.Like this, emptying and exhaust take full advantage of complete stroke and inside cylinder 1.1 and piston 5 and can determine specification as small as possible.
It will be apparent to those skilled in the art that and determine that specification also can carry out as follows, that is, a stroke is enough to more than 8 emptying of capillary.For with capillary 8 exhausts and recharge, then piston component 15 on the direction of the upper end position of piston component 15, only promote a bit, thereby capillary 8 is opened.
Because the pressure in inside cylinder 1.1 is not unexpected rising and only is smaller yet, so liquid also may be released in the container of existing liquid, and so-called crosstalking can not appear thus, as described in the explanation of prior art.When being provided with valve 14 in being connected between inside cylinder 1.1 and the capillary 8, it is possible that pulsed is emitted.
Basically, where closed piston 5 is unessential for the mode of action of piston cylinder units 17.This is can be on the bottom that piston 5 guides in inside cylinder 1.1 on the principle, any position in the upper end of piston 5 or preferably carry out in the upper end of piston 5.
Preferably seal by piston sealing device.Piston 5 or directly have end face that rounding ground, advantage ground makes up by putting on the piston sealing device with being structured on the upper end, otherwise the sealing of piston 5 directly by will around the end setting of piston 5, undertaken by the mode of putting piston seal and opening with respect to its peripheral piston head 5.1 that seals.The scheme of mentioning at last has following advantage,, can provide wideer by putting face by piston head 5.1 being determined sizes that is.Piston sealing device preferably may be embodied as piston seal plate 6, this piston seal plate by elastomeric material for example synthetic rubber constitute.The sealing of all pistons 5 utilizes identical piston seal plate 6 to carry out with having advantage then.Instead, also can use each cone or spheroid 12 as piston sealing device.These cones or spheroid 12 can for example be constituted or equally preferably can be made of elastomeric material by glass, pottery.By piston head 5.1, piston 5 is suspended in the piston component 15.
Next several embodiment that distinguish as the different structural form of implementation of a seal, piston head 5.1, valve 14 and capillary retainer 7 by various different parts are described.The different forms of implementation energy combinations with one another of different parts are not even mention each that may make up here in detail.
A kind of embodiment with advantage that meets the most the requirement of the precision of emitting minimum volume and rapidity is the capillary dispenser that has a large amount of capillaries 8, the groove of the microtiter plates of these capillaries and commercial standard (CS) correspondingly is arranged in the matrix each other, and has a piston cylinder units 17 for each capillary 8.
In first embodiment, to set forth such capillary dispenser with reference to Fig. 1-3.
The capillary dispenser that having shown in Fig. 1 a is the capillary 8 that rectangular arranges has loading plate 1, pressing plate 2 and piston holding plate 3 and rests on storehouse casket 9 on the loading plate 1 by sealing gasket 10 indirectly, in this storehouse casket capillary retainer 7 together with capillary 8 with hang, force closure rest on the sealing gasket 10.
Be provided with a piston cylinder units 17 for each capillary 8, piston cylinder units 17 is arranged on the axis A.In Fig. 1 a, exemplarily drawn an axis A among the axis A.In order to see piston holding plate 3, the piston seal plate 6 that hides piston holding plate 3 and the baffle plate 4 that is arranged on the piston seal plate 6 are not shown in Fig. 1 a, they are attached troops to a unit equally in piston cylinder units 17.
In the portion of leaving a blank shown in the loading plate 1 at (assembly that automatic operating is required such as Control Component) motor and transmission device and arrange.Loading plate 1 is formed on the downside of this unshowned housing simultaneously.
The different embodiment of piston cylinder units 17 can see as the cross section diagram in Fig. 2 to Fig. 5.
First form of implementation of piston cylinder units 17 has been shown in Fig. 3 a and Fig. 3 b, wherein, the piston component 15 that is in the upper end position has been shown in Fig. 3 a and the piston component 15 that is in the lower end position has been shown in Fig. 3 b.
The inside cylinder 1.1 of piston cylinder units 17 is formed by the reach through hole in loading plate 1.Reach through hole can be the hole of classification as shown, and it comprises: have the section of big interior diameter, piston 5 moves in this section; With the section that has than minor diameter, in order to form passage, the diameter of this passage is greater than the diameter of capillary 8, but less than the diameter that is structured in the tip bundle 7.2 on the capillary retainer 7.The length of the section that piston 5 moves therein depends on stroke and piston head 5.1 to be had end play and selects in the recess 3.1 of piston plate 3, the length of this section is combined with the diameter of piston 5 and is determined the maximum volume that sends.The grid that capillary 8 is arranged in wherein is more little, must determine the diameter of inside cylinder 1.1 more little inevitably and therefore the length of the section that moves therein of piston 5 must be more big.
For piston 5 is sealed with respect to inside cylinder 1.1, between pressing plate 2 and loading plate 1, be provided with the seal sleeve 11 that piston 5 sealings are surrounded.
Have to be rectangular arrange the loading plate 1 of the inside cylinder 1.1 that makes up, have with the common cylinder assembly 16 that forms with capillary 8 equal numbers that exist of the seal sleeve 11 of the pressing plate 2 of the reach through hole of inside cylinder 1.1 identical set and respective numbers.
The piston 5 of piston cylinder units 17 is following hollow pistons, installs or be built with piston head 5.1 in the upper end of this hollow piston.Piston head 5.1 surrounds the upper end of piston 5 as the axle collar, and has two perpendicular to end face 5.1.1 and the 5.1.2 of axis A distribution.Piston head 5.1 can lie in the recess 3.1 of piston holding plate 3 by lower surface 5.1.1, and this recess is darker than the height of piston head 5.1.Thus, piston head 5.1 has end play in the direction of axis A, and this gap equals poor between the degree of depth of the height of piston head 5.1 and recess 3.1.Upper surface 5.1.2 protrudes the upper end that surpasses piston 5, and upper surface 5.1.2 can be by putting on the piston sealing device that is embodied as piston seal plate 6 thus.
Basically, the upper end of piston 5 also can be protruded above upper surface 5.1.2, and the upper end of piston 5 can be by putting on the piston seal plate 6 thus.
As it is evident that from Fig. 1 a, the upside of piston holding plate 3 has in a large number the recess 3.1 with the grid form that be rectangular identical with capillary 8.The recess of arranging 3.1 that is in line respectively is connected to each other by groove 3.2, and these grooves form air duct jointly with the piston sealing device that keeps flat that is embodied as piston seal plate 6.On piston seal plate 6, be furnished with baffle plate 4, this baffle plate indirectly by piston seal plate 6 via being fastened on the piston holding plate 3 of can getting loose.
Piston holding plate 3 and baffle plate 4 can move with respect to fixed loading plate 1 along axis A jointly, and piston 5 can correspondingly promote with the length of stroke and reduce in inside cylinder 1.1 thus.
Piston holding plate 3, piston seal plate 6, baffle plate 4 and the piston component 15 with piston 5 common capillary 8 equal numbers that form and exist of the piston head 5.1 that is separately positioned on it.
Attach troops to a unit and be connected with pneumatic mode in the capillary 8 of piston cylinder units 17 and inside cylinder 1.1.Basically can consider the connection of following any kind at this, this connection is the connection with respect to the surrounding environment sealing.
Is connected with the capillary dispenser in order to be the capillary 8 that rectangular arranges, capillary 8 introducings have each other accordingly in the storehouse casket 9 of grid spacing, and this storehouse casket 9 rests on the loading plate 1 by sealing gasket 10 indirect force sealedly.Next, term " storehouse casket " 9 will represent following surface plate, is furnished with capillary 8 in this surface plate, does not rely on how these capillaries remain in the plate and whether they releasably can not adhere to ground or non-cohesive remaining in the plate in other words with getting loose.
In the embodiment that does not more have advantage, capillary 8 can for example enter in the storehouse casket 9 securely.In this shortcoming be, capillary 8 impaired, that break or that block can not be replaced, and this needs to change whole storehouse casket 9 in case of necessity.
Instead, capillary 8 also can be inlaid in the bar (for example being made of plastics) individually, and by be arranged on this bar get loose be threaded, be clamped and connected or socket connection is releasably secured in the storehouse casket 9.Apparent advantage is for above-mentioned solution, replaces separately and simply the capillary 8 with bar, is connected to and/or the force closure sealed with storehouse casket 9 shapes of this bar.Shortcoming is, this connection can not at random frequently get loose and not wear and tear, and the suction-combining force that is used for reinforcing this connection neither allow excessive do not allow yet too small, in order to do not damage bar and do not guarantee firm cooperation on the other hand on the one hand.
The solution with advantage that is used for setting-in capillary 8 proposes these capillaries are introduced capillary retainer 7 respectively, and this capillary retainer is implemented as the pipettor tip and shown in Fig. 3 a and the 3b.The capillary retainer is made of the tip portion 7.1 of tubulose and the tip bundle 7.2 that is structured on this tip portion 7.1 end after the dispensing direction is leaned on, and this tip bundle has rear end side and the front towards tip portion 7.1.
Capillary 8 preferably is made of glass, but also can by other materials for example plastics or pottery constitute, wherein, what must guarantee is to want the capillary rise height of the liquid of dispensing to be enough to capillary 8 is filled fully.Capillary rise height h calculates by following formula:
At this, γ is the surface tension of liquid, and θ is angle of wetting, and ρ is density of liquid, and g is that acceleration of gravity and R are the inside radius of capillary 8.
Capillary 8 preferably has hydrophobic external coating, in order to stop liquid to adhere to when mentioning from liquid as far as possible and suppress liquid and climb in the outside of capillary 8 when capillary end forms drop.What have advantage especially is the coating that fluoropolymer constitutes.
Have advantage ground, capillary 8 or its free end thin-walled ground are as far as possible implemented, and minimize and make thus the adhesive capacity of liquid on end face to minimize in order to make at the end face on the capillary end.
Next by the working method as setting forth according to capillary dispenser of the present invention at the capillary 8 shown in the function combination of the piston cylinder units 17 as shown in Fig. 3 a and Fig. 3 b.
When the dispensing process began, piston component 15 was in the upper end position, as shown in Fig. 3 a.Piston head 5.1 is on the bottom of the recess 3.1 in the piston holding plate 3.In piston 5, its lower end is in inside cylinder 1.1, and its upper end is connected with atmosphere by air duct, and therefore ambient pressure is in leading in inside cylinder 1.1.
Because capillary 8 can independently be filled by its free end of submergence by its capillarity, as long as the other end be open and apply and essentially identical ambient pressure on free end, so capillary 8 can be filled or be filled by being immersed in the liquid now.The container that for this reason preferably will be filled with liquid promotes towards capillary 8.
For dispensing, in inside cylinder 1.1, must set up pressure, in order to discharge liquid from capillary 8, precondition is that inside cylinder 1.1 is closed and the free volume in inside cylinder 1.1 then is reduced.The sealing of inside cylinder 1.1 is undertaken by the one-sided sealing of piston 5 indirectly, and its mode is that piston component 15 is reduced.After the path of the end play that equals piston head 5.1, piston head 5.1 is pressed against on the baffle plate 4 by piston seal plate 6 indirectly and is closed.Piston cylinder units 17 seals with respect to surrounding environment thus, and sets up for the pressure of liquid from capillary 8 dispensings at piston component 15 and under the situations that piston 5 further reduces thus, is enough to discharge liquid until this pressure.To this referring to Fig. 3 b.The lower end position of piston component 15 is given by piston holding plate 3 is rested on the pressing plate 2, but can limit by the additional backstop that exists too.
After emitting liquid fully, piston 5 is brought in its upper end position again by the lifting of piston component 15, and wherein, piston 5 is opened again, thereby makes occur ambient pressure again in inside cylinder 1.1.At this or then, can reuptake liquid by capillary 8, wherein, absorb not by suction but only cause by capillary effect.Described working method is carried out synchronously for all piston cylinder units 17 of capillary dispenser.
In a second embodiment, the capillary dispenser will have the capillary 8 for piston cylinder units 17 integral multiples, as them once being described among first embodiment.
In the case, be connected with quantity corresponding to the capillary 8 of piston cylinder units 17 several times with each inside cylinder 1.1.Along with the increase of quantity of wanting the capillary 8 of emptying by piston cylinder units 17, in inside cylinder 1.1, must set up bigger pressure.
Replace the piston seal plate 6 as the piston sealing device that is used for all piston cylinder units 17, can use also that for example spheroid 12 is with piston 5 sealings at each piston cylinder units 17, this spheroid is placed in the recess 13.1 in additional accessory plate 13.
In Fig. 4 a and 4b, illustrated and had as the intercept of piston sealing device at the piston component 15 of the upper end position of piston component 15 and the spheroid in the lower end position 12.
Identical with embodiment described above, in the lower end position of piston component 15, piston head 5.1 rests in recess 3.1 on its bottom, and piston 5 is opened.
In the upper end position of piston component 15, the upper end of piston 5 is directly by putting on the spheroid 12 and piston 15 seals.Spheroid 12 can be made by the synthetic rubber manufacturing or by the pottery with high-precision surface, glass or metal.Under the situation of stiff spheroid 12, the end face with the radius of curvature that equates with radius of sphericity is made in the upper end of piston 5.Spheroid 12 is placed in accessory plate 13 on each piston 5 respectively in the recess 13.1 that for this reason arranges.In piston holding plate 3, also be provided with groove 3.2 in the present embodiment.These grooves correspondingly also can be introduced in the side of adjacency of accessory plate 13.
Another embodiment will set forth with reference to Fig. 5.
For dispensing contactlessly, as having set forth in the description of prior art, need high piston acceleration/piston speed, so that the corresponding required pressure of dispensing of setting up rapidly.This can realize by big piston diameter.If yet each inside cylinder 1.1 is attached troops to a unit in capillary 8 only for this reason, structure space limits by the grid of capillary 8 so, the grid of the groove of this grid and Target Board (96 formula plate=9mm; 384 formula plate=4.5mm; 1536 formula plates=2.25mm) mate.
In order under the situation that does not have high acceleration/speed, to deal with, also valve 14 can be placed on the bottom of inside cylinder 1.1.Can in inside cylinder 1.1, set up the required pressure of dispensing by piston 5 by valve 14, and discharge as pressure pulse then.For this reason, can consider the small electrical magnet valve, and passive spheroid check-valves and so-called cross valve or the pressure bell (Druckbills) that is constituted by synthetic rubber.Problem is under the situation of passive valves, they usually with pressure not as pulse release, but when reaching threshold value, open lentamente.In order to utilize passive valves also can produce pressure pulse, propose to determine as follows the specification of valve 14, that is, it also is closed making under the situation of its maximum pressure that can produce in inside cylinder 1.1 always, and mechanically open by applying just of piston 5, thereby pressure can be released suddenly.
Reference numerals list
1 loading plate
1.1 inside cylinder
2 pressing plates
3 piston holding plates
3.1 recess
3.2 groove
4 baffle plates
5 pistons
5.1 piston head
5.1.1 the lower surface of piston head
5.1.2 the upper surface of piston head
6 piston seal plates
7 capillary retainers
7.1 tip portion
7.2 most advanced and sophisticated bundle
8 capillaries
9 storehouse caskets
10 sealing gaskets
11 seal sleeves
12 spheroids
13 accessory plates
13.1 recess
14 valves
15 piston components
16 cylinder assemblies
17 piston cylinder units
The A axis
Claims (9)
1. capillary dispenser, it has at least one piston cylinder units (17), described piston cylinder units (17) is by the cylinder assembly with inside cylinder (1.1) (16) and have piston component (15) formation of piston (5), they are arranged on the axis (A) jointly, wherein, described inside cylinder (1.1) is connected with pneumatic mode with capillary (8) at least, wherein, described piston (5) is embodied as the hollow piston opened both sides and can moves between the upper end position of described piston component (15) and lower end position along described axis (A) in described inside cylinder (1.1), and wherein, described piston (5) can one-sided sealing, so as towards the lower end position of described piston component (15) between moving period with the one-sided sealing of described piston (5).
2. capillary dispenser according to claim 1, it is characterized in that, piston sealing device belongs to described at least one piston component (15), force closure ground is by putting on the described piston sealing device in the upper end position of described piston component (15) in the upper surface of described piston (5), and described piston (5) sealing and described piston (5) have end play in the inner direction at described axis (A) of described piston component (15) thus.
3. capillary dispenser according to claim 1, it is characterized in that, piston sealing device belongs to described at least one piston component (15), and there is piston head (5.1) at described piston (5), described piston head (5.1) protrudes the upper surface that surpasses described piston (5) and force closure ground is by putting on the described piston sealing device in the upper end position of described piston component (15), and described piston (5) sealing and described piston (5) have end play in the inner direction at described axis (A) of described piston component (15) thus.
4. according to claim 2 or 3 described capillary dispensers, it is characterized in that described piston sealing device is spheroid (12), and the upper surface of described piston (5) is built with the radius of curvature that equates with the radius of described spheroid (12).
5. according to claim 2 or 3 described capillary dispensers, it is characterized in that described piston sealing device is piston seal plate (6).
6. capillary dispenser according to claim 1 is characterized in that, described inside cylinder (1.1) is connected by valve (14) with described capillary (8).
7. capillary dispenser according to claim 1 is characterized in that, at least one capillary (8) is pressed in the capillary retainer (7), and described capillary retainer has the form of pipette tip.
8. capillary dispenser according to claim 1, it is characterized in that, described capillary dispenser comprises a large amount of capillaries (8), and the capillary retainer (7) that has a pipette tip form for each capillary (8), the mode that described capillary (8) protrudes with free end, insert in the described capillary retainer (7) securely, and described capillary retainer (7) is arranged in the storehouse casket (9) in the mode that hangs.
9. capillary dispenser according to claim 1 is characterized in that, each inside cylinder (1.1) is connected with a plurality of capillaries (8).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102012100836.1 | 2012-02-01 | ||
| DE201210100836 DE102012100836B3 (en) | 2012-02-01 | 2012-02-01 | Kapillardispenser |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103240136A true CN103240136A (en) | 2013-08-14 |
| CN103240136B CN103240136B (en) | 2016-06-22 |
Family
ID=47594533
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310042248.XA Expired - Fee Related CN103240136B (en) | 2012-02-01 | 2013-02-01 | Capillary dispenser |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP2623203B1 (en) |
| CN (1) | CN103240136B (en) |
| DE (1) | DE102012100836B3 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105705241A (en) * | 2013-11-07 | 2016-06-22 | 吉尔松有限合伙公司 | Positive displacement pipetting system, having a design facilitating the gripping of the piston of the capillary-piston assembly |
| CN113135306A (en) * | 2021-04-30 | 2021-07-20 | 杭州博日科技股份有限公司 | Automatic filling device and method for magnetic beads and reagent |
| CN113135328A (en) * | 2021-04-30 | 2021-07-20 | 杭州博日科技股份有限公司 | Full-automatic liquid subpackaging production line |
| CN115135417A (en) * | 2019-10-25 | 2022-09-30 | 梅特勒-托利多瑞宁有限责任公司 | Syringe for electric external piston type pipettor |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109990860B (en) * | 2019-04-18 | 2024-03-19 | 山东省科学院海洋仪器仪表研究所 | Pneumatic capillary clamping device |
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|---|---|---|---|---|
| US4140488A (en) * | 1977-09-06 | 1979-02-20 | Hycel, Inc. | Sample liquid transfer means in an automatic chemical testing apparatus |
| EP1506814A1 (en) * | 2003-08-13 | 2005-02-16 | The Automation Partnership (Cambridge) Limited | Pipetting Head |
| CN1669657A (en) * | 1999-12-08 | 2005-09-21 | 吉尔森公司 | Pipet for extracting and dispensing liquid sample |
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|---|---|---|---|---|
| US5059398A (en) * | 1985-07-22 | 1991-10-22 | Drummond Scientific Company | Disposable preselected-volume capillary pipet device |
| US5508200A (en) * | 1992-10-19 | 1996-04-16 | Tiffany; Thomas | Method and apparatus for conducting multiple chemical assays |
| DE19742005A1 (en) * | 1997-09-24 | 1999-04-01 | Microdrop Ges Fuer Mikrodosier | Disposable-capillary micropipette forming part of multiple unit used in combinatorial investigations |
| GB2368640B (en) * | 1999-08-13 | 2003-09-17 | Cartesian Technologies Inc | Apparatus for liquid sample handling |
| CH695544A5 (en) * | 2000-11-17 | 2006-06-30 | Tecan Trading Ag | Apparatus for dispensing or aspirating / dispensing liquid samples. |
| FI20012167A (en) * | 2001-11-09 | 2003-05-10 | Antti Manninen | Pipette or syringe |
| US20040141885A1 (en) * | 2002-02-12 | 2004-07-22 | Molecular Devices Corp. | Pipettor systems and components |
| WO2005007289A2 (en) * | 2003-07-11 | 2005-01-27 | Genomic Solutions Inc. | Apparatus and methods for liquid sample handling based on capillary action |
-
2012
- 2012-02-01 DE DE201210100836 patent/DE102012100836B3/en not_active Expired - Fee Related
-
2013
- 2013-01-10 EP EP20130150872 patent/EP2623203B1/en active Active
- 2013-02-01 CN CN201310042248.XA patent/CN103240136B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4140488A (en) * | 1977-09-06 | 1979-02-20 | Hycel, Inc. | Sample liquid transfer means in an automatic chemical testing apparatus |
| CN1669657A (en) * | 1999-12-08 | 2005-09-21 | 吉尔森公司 | Pipet for extracting and dispensing liquid sample |
| EP1506814A1 (en) * | 2003-08-13 | 2005-02-16 | The Automation Partnership (Cambridge) Limited | Pipetting Head |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105705241A (en) * | 2013-11-07 | 2016-06-22 | 吉尔松有限合伙公司 | Positive displacement pipetting system, having a design facilitating the gripping of the piston of the capillary-piston assembly |
| CN115135417A (en) * | 2019-10-25 | 2022-09-30 | 梅特勒-托利多瑞宁有限责任公司 | Syringe for electric external piston type pipettor |
| CN113135306A (en) * | 2021-04-30 | 2021-07-20 | 杭州博日科技股份有限公司 | Automatic filling device and method for magnetic beads and reagent |
| CN113135328A (en) * | 2021-04-30 | 2021-07-20 | 杭州博日科技股份有限公司 | Full-automatic liquid subpackaging production line |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103240136B (en) | 2016-06-22 |
| EP2623203A1 (en) | 2013-08-07 |
| DE102012100836B3 (en) | 2013-04-11 |
| EP2623203B1 (en) | 2015-03-18 |
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