CN103191479B - Optimization method for continuous centrifugal blood separation in curved-surface container - Google Patents
Optimization method for continuous centrifugal blood separation in curved-surface container Download PDFInfo
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Abstract
The invention relates to an optimization method for continuous centrifugal blood separation in a curved-surface container. The method comprises the following steps: a separation cavity gap is formed in a container, and adopts the structure of a curved surface; a separation soft bag is arranged inside the separation cavity gap; blood is fed into the separation soft bag, and is led to rotate at high speed around an rotation axle along with the curved-surface container; different blood ingredients are separated from the blood as per density under the action of a centrifugal force; the continuous centrifugal blood separation is performed in the separation soft bag; and the rotation direction of the curved-surface container is the same as the unfolding direction of the separation cavity gap. According to the method provided by the invention, the separation soft bag and the curved-surface container are used together, and the direction of centrifugal rotation is controlled, so that the continuous centrifugal separation of the blood ingredients is realized; and the method has the optimal advantage of capability of increasing the separation quality without increasing separation cost.
Description
Technical field
The present invention relates to blood constituent to be separated, particularly relate to one and continuous centrifugal separation is carried out to blood, thus isolate the optimization method of the visible component such as blood plasma and blood cell.
Background technology
No matter scientific research or medical clinical practice or commercial production, and more occasion, all need blood separation, as gone out single component from separation of whole blood, usual use centrifuging isolates various single component from blood, for occasions such as clinical treatment, scientific research or raw materials.Modal is that whole blood is isolated erythrocyte, granulocyte, mononuclear cell, platelet and blood plasma by centrifugal separation system, maybe erythrocyte and cleaning mixture is isolated in the FRC washing after thawing.
The operation principle of continuous centrifugal separation system is: the primary structure of said system includes centrifuge, infusion pump and control device; Blood introduced in the soft bag on centrifuge in knock-out drum by the infusion pipeline that is connected with infusion pump, high speed rotating knock-out drum, and drive this soft bag synchronous high-speed to rotate, the not same-action that blood in it is subject to centrifugal force field causes the visible component such as blood plasma and blood cell to do centrifugal sedimentation campaign, and presses the size layering of respective density or proportion or sedimentation coefficient; When reaching sedimentation equilibrium, being arranged each single component layer forming concentric circles from the periphery of radial direction to axle center by the high to Low enrichment of density, then utilizing infusion pump to be extracted by the single component layer of separation again.
It is the effect providing rotary power by airtight flexible pipe and play input continuously and extract out that blood single component realizes continuous acquisition within the system, one end of flexible pipe is communicated with the soft bag that is separated in knock-out drum, with knock-out drum high-speed rotation, the other end of flexible pipe is fixed on support, therefore, rotation one end, flexible pipe one end is fixed, coil arrangement is had in the middle of it to twine to realize flexible pipe solution of untwisting, make whole blood to be inputed in the knock-out drum of rotation under rotation status, and extract the single component such as blood plasma and blood cell out in the knock-out drum rotated.Knock-out drum, i.e. separator disk, combine with coil arrangement and achieve blood continuous centrifugal and be separated.
Relate in prior art be applied to separator disk on blood continuous centrifugal separation equipment and coil arrangement mainly contain US Patent No. 5360542.In that patent, separator disk be one columnar structured, be called knock-out drum, have a columnar lacuna in it, soft bag is placed on the centrifugalize realizing blood in circular lacuna; Coil arrangement includes underframe, rotatable top-support, top-support suspends knock-out drum in midair, extend into bottom knock-out drum a flexible pipe is secured across two bearings of top-support side from cabinet place after, the end of its flexible pipe is a square spigot, extend in the square groove of knock-out drum central axis.Flexible pipe is hollow structure, has several transfer pipeline in it, and realize power provides and Liquid transfer function simultaneously.Based on above-mentioned structure, power makes top-support rotate, and drives flexible pipe untwist and produce torque forces, and is made it by this power transmission to knock-out drum to produce rotating in same direction, and then realizes the separation process of blood continuous centrifugal.
The separator disk of another version is disclosed in Chinese patent application 200710046991.7.Point out in this patent: the separator disk in many cells composition mixing material piece-rate system, comprise durable hard-caked sediment dish and disposable soft bag, the circular discs that hard-caked sediment dish is made up of inner core and base, formed between inner core and base one section around this hard-caked sediment dish circle core shaft one week and the continuous lacuna do not closed of stem and afterbody, soft bag is for being provided with the single cavity structure of liquid inlet and outlet pipe, and this soft bag can be inserted in described lacuna.Because centrifugal force everywhere in described continuous lacuna is inconsistent, therefore under centrifugal force continuous action, each composition of mixing material in segmentation distribution, can extract corresponding single component in soft bag in above-mentioned segmentation.Above-mentioned inner core is equivalent to described interior cylinder, base is equivalent to described outer cylinder.Above-mentioned hard-caked sediment dish that is to say separator disk.
No matter be foreign patent or domestic patent, all have employed the mode that separator disk adds soft bag, soft bag is disposable consumable component part.The separator disk of these the two kinds of forms mentality of designing in actual use due to blood separation is different, and the former be concentric design, and the latter designs for non-concentric, and the separation efficiency of the latter improves a lot compared with the former.Though the latter is comparatively ingenious with the design being separated soft bag at hard-caked sediment dish, and has certain effect in practice, but still has the leeway of Curve guide impeller.
Summary of the invention
The object of the invention is to the deficiency overcoming the existence of above-mentioned prior art, the optimization method that a kind of new blood continuous centrifugal is separated is provided.Method of the present invention is intended to realize, in the separation of efficient blood continuous centrifugal, by controlling centrifugal rotation direction, not increasing separation costs, and improving the quality of separated component.
In order to reach foregoing invention object, technical scheme provided by the invention is as follows:
A kind of blood optimization method that continuous centrifugal is separated in curved body container, it is characterized in that, be provided with separation lacuna in described curved body container, this curved body container, around rotating shaft high speed rotating, utilizes centrifugal force that the blood be injected in described separation lacuna is gone out heterogeneity by density size separation; Described separation lacuna is the curved body including outer wall and inwall, and the outer wall curved surface of this separation lacuna is as follows with polar coordinate representation perpendicular to the curve that the plane of rotating shaft projects:
Wherein, polar limit O is the intersection point of rotating shaft and described plane, and polar pole axis L is the ray that limit arrives curve initiating terminal direction, the positive direction of polar angle is clockwise, r is the footpath, pole of any point on curve, and R is the footpath, pole of curve distalmost end, θ
1for the polar angle of curve initiating terminal, its value is 0 degree of this polar coordinate system, θ
2for curve and blood plasma separating factor critical radius F
rthe polar angle of intersection point, blood plasma separating factor critical radius F
rfor blood plasma in unit interval when rotating speed is determined is separated required minimum centrifugal radius, θ
3be a polar angle, its value is θ
21.5 ~ 3.5 times, θ
4for the polar angle of curve distalmost end; In described separation lacuna, [θ
1, θ
2) be blood plasma district, [θ
2, θ
3) be chaotic region, [θ
3, θ
4] be blood cell district, b
1for the curve coefficients in blood plasma district, b
2for the curve coefficients of chaotic region, b
3for the curve coefficients in blood cell district; The direction of rotation of described separation container is identical with the expansion direction being separated lacuna.
In the blood of the present invention optimization method that continuous centrifugal is separated in curved body container, described curve coefficients refers to the rate of change of footpath, pole relative to polar angle of any point on curve.
In the blood of the present invention optimization method that continuous centrifugal is separated in curved body container, the expansion direction of described separation lacuna refers to: in the blood plasma district of described curve, have any two points p and q, and its polar angle is respectively θ p and θ q, corresponding footpath, pole is respectively rp and rq, and θ
1≤ θ p< θ q≤θ
2, rp<rq simultaneously, some p are described curve expansion direction along curved trajectory to the direction of some q.
In the blood of the present invention optimization method that continuous centrifugal is separated in curved body container, the continuous centrifugal separation process of described blood specifically comprises the steps:
The first step, curved body container described in high speed rotating, be placed in the soft bag of the separation high speed rotating be thereupon separated in lacuna, the front portion of the soft bag of this separation, middle part, rear portion are respectively equipped with the flexible pipe that is carried liquid, the front portion being separated soft bag is positioned at the nearer near-end of disengagement chamber gap distance rotating shaft, the middle part being separated soft bag is positioned at the middle part being separated lacuna, and the rear portion being separated soft bag is positioned at disengagement chamber gap distance rotating shaft far-end far away;
Second step, blood is injected into the soft bag of separation by being positioned at the flexible pipe be separated in the middle part of soft bag, be full of behind the soft bag space of separation until whole blood, be separated soft bag and be separated lacuna and fit tightly the inner separation space formed with being separated lacuna and being close, blood in the soft bag of separation by various composition segmentation and regionalization gradually under centrifugal force continuous action, blood plasma is gathered in gradually and is separated soft bag front portion and forms a blood plasma district, the visible component comprising blood cell is gathered in gradually and is separated soft bag rear portion and forms a blood cell district, and be separated lasting input formation chaotic region due to whole blood, middle part of soft bag,
3rd step, blood plasma is extracted out in blood plasma district from being arranged on the flexible pipe being separated soft bag front portion, the visible component comprising blood cell is extracted out in blood cell district from being arranged on the flexible pipe being separated soft bag rear portion, continue input whole blood from being arranged on the flexible pipe be separated in the middle part of soft bag simultaneously, reach with extraction the dynamic equilibrium being separated soft bag inner capacities by control inputs, and then the continuous centrifugal realizing blood is separated.
Based on foregoing invention content, the present invention carries out having following technique effect in the method for continuous centrifugal separation compared with method of the prior art to blood:
1. the present invention is in order to realize the continuous high-efficient centrifugalize of blood, propose the concept that curved surface continuous centrifugal is separated, and the curved body container of an applicable continuous centrifugal separation is devised based on this thought, this curved body container is embodied in the separation lacuna inside a separator disk, be separated in lacuna arrange one fit with the inside and outside wall being separated lacuna be separated soft bag, and be separated the flexible pipe soft bag being arranged input and extracted liquid, the flexible pipe extracting heterogeneity is arranged in different subregions, a total input pipe is set again, blood is placed in this soft bag and carries out high speed rotating, under the influence of centrifugal force, segmentation and regionalization is carried out according to density difference in the separated space that blood can be formed at curved surface, so namely, by the dynamic equilibrium inputted and extract out, realize the continued operation of blood separation, improve the separation efficiency of blood.
2. the present invention is in order to reach the object of the optimization continuous high-efficient centrifugalize of blood, design and pass through to control the direction of rotation of continuous curve surface body container, namely the direction of rotation of this container is identical with the expansion direction being separated lacuna, and realizing does not increase separation costs, and improves the quality of separated product.
Accompanying drawing explanation
Fig. 1 is separated the projection of lacuna curved surface and the schematic diagram of direction of rotation in the optimization method of blood continuous centrifugal of the present invention separation.
Fig. 2 is the structural representation of centrifugal separation system.
Fig. 3 is the structural representation of separator disk in the present invention.
Fig. 4 is the deployed configuration schematic diagram being separated soft bag in the present invention.
Fig. 5 be the present invention be separated that blood in soft bag just entered separated space flow to schematic diagram.
Fig. 6 be the present invention be separated blood in soft bag be full of separated space before flow to schematic diagram.
Fig. 7 be the present invention be separated blood in soft bag be full of separated space after flow to schematic diagram.
Fig. 8 be direction of rotation of the present invention contrary with expansion direction time, the three-dimensional stressing conditions schematic diagram of the visible component such as blood cell in separated space.
Fig. 9 be direction of rotation of the present invention contrary with expansion direction time, the plane stressing conditions schematic diagram of the visible component such as blood cell in separated space.
Figure 10 be direction of rotation of the present invention identical with expansion direction time, the three-dimensional stressing conditions schematic diagram of the visible component such as blood cell in separated space.
Figure 11 be direction of rotation of the present invention identical with expansion direction time, the plane stressing conditions schematic diagram of the visible component such as blood cell in separated space.
Detailed description of the invention
With specific embodiment, the method that blood continuous centrifugal of the present invention is separated being described in further detail below in conjunction with accompanying drawing, in the hope of understanding operation principle of the present invention and workflow more lucidly, but can not limiting the scope of the invention with this.
The invention belongs to a kind of method that blood continuous centrifugal is separated, for convenience of explanation, be separated into example with the continuous centrifugal of blood plasma in whole blood here and set forth.The party's ratio juris blood is placed in one to have the separation container being separated lacuna, by this separation container of high speed rotating, thus blood plasma in blood and other visible components separated.Certainly, except erythrocyte is separated with blood plasma, the separation of other visible components in blood can also be used for.Blood Midst density difference is erythrocyte and blood plasma to the maximum, and wherein erythrocytic density is maximum, and the density of blood plasma is minimum, and the blood plasma of single component and erythrocyte are the blood constituents that in medical science, expense is maximum.Therefore, carrying out continuous centrifugal separation to the blood plasma in blood is the most basic, the simplest component separating.
If realize isolating blood high-density composition and low-density composition continuously and efficiently, first innovative design to be carried out to centrifugalize container.The present invention first have devised one and is separated lacuna in separation container, the shape of this lacuna is the curved body including outer wall and inwall, separation container containing this curved body lacuna is called curved body container, can be reached the object of blood constituent high-efficiency and continuous centrifugalize by the application of this container.The outer wall curved surface of above-mentioned separation lacuna is as follows with polar coordinate representation perpendicular to the curve that the plane of rotating shaft projects:
Wherein, polar limit O is the intersection point of rotating shaft and described plane, and polar pole axis L is the ray that limit arrives curve initiating terminal direction, the positive direction of polar angle is clockwise, r is the footpath, pole of any point on curve, and R is the footpath, pole of curve distalmost end, θ
1for the polar angle of curve initiating terminal, its value is 0 degree of this polar coordinate system, θ
2for curve and blood plasma separating factor critical radius F
rthe polar angle of intersection point, blood plasma separating factor critical radius F
rfor blood plasma in unit interval when rotating speed is determined is separated required minimum centrifugal radius, θ
3be a polar angle, its value is θ
21.5 ~ 3.5 times, θ
4for the polar angle of curve distalmost end; In described separation lacuna, [θ
1, θ
2) be blood plasma district, [θ
2, θ
3) be chaotic region, [θ
3, θ
4] be blood cell district, b
1for the curve coefficients in blood plasma district, b
2for the curve coefficients of chaotic region, b
3for the curve coefficients in blood cell district.The form of expression due to drop shadow curve is a helix, therefore defines key point wherein with footpath, pole, polar angle.Curve coefficients refers to the rate of change of footpath, pole relative to polar angle of any point on curve.As shown in Figure 1, Fig. 1 is the perspective view being separated lacuna curved surface in the method for blood continuous centrifugal of the present invention separation to the general shape of above-mentioned curve.Extract desired blood component out in the separation lacuna of curved body container while, constantly supplement whole blood again, make separation lacuna inner capacities reach a balance dynamically, and then realize the blood continuous centrifugal separation process in the object of the invention.
The structure of curved body container includes the separation lacuna of curved body form, and this Vessel Design is a disc-shaped structure, is referred to as separator disk.This separator disk includes interior cylinder 1 and outer cylinder 2 two parts.Interior cylinder 1 is removably fixed in the inner chamber of outer cylinder 2, leaves certain space between the internal chamber wall of outer cylinder 2 and the lateral wall of interior cylinder, and this space is exactly the separation lacuna 3 on separator disk, as shown in Figure 3.Receive the soft bag of separation that once property uses being separated lacuna content, the process that blood continuous centrifugal is separated is carried out in the soft bag of separation.
The internal chamber wall of above-mentioned outer cylinder is the near-end and the far-end that include distance central rotation axis different distance, and be a curved surface between described near-end and far-end, this curved surface is being expressed as follows by polar form perpendicular to the drop shadow curve in the plane of rotating shaft:
Wherein, polar limit O is the intersection point of rotating shaft and described plane, and polar pole axis L is the ray that limit arrives curve initiating terminal direction, the positive direction of polar angle is clockwise, r is the footpath, pole of any point on curve, and R is the footpath, pole of curve distalmost end, θ
1for the polar angle of curve initiating terminal, its value is 0 degree of this polar coordinate system, θ
2for curve and blood plasma separating factor critical radius F
rthe polar angle of intersection point, blood plasma separating factor critical radius F
rfor blood plasma in unit interval when rotating speed is determined is separated required minimum centrifugal radius, θ
3be a polar angle, its value is θ
21.5 ~ 3.5 times, θ
4for the polar angle of curve distalmost end; In described separation lacuna, [θ
1, θ
2) be blood plasma district, [θ
2, θ
3) be chaotic region, [θ
3, θ
4] be blood cell district, b
1for the curve coefficients in blood plasma district, b
2for the curve coefficients of chaotic region, b
3for the curve coefficients in blood cell district.Above-mentioned curved surface is exactly the specific implementation form as being separated lacuna lateral wall.
Above-mentioned interior cylinder 1 and outer cylinder 2 coaxial combination form entirety separator disk as shown in Figure 3.Generally, lateral wall and the internal chamber wall of outer cylinder of interior cylinder can be designed as has different curved form or identical curved form, if the latter, form a thickness within it between cylinder lateral wall and outer cylinder internal chamber wall and be separated lacuna 3 uniformly.
Place one in separation lacuna in described continuous curve surface separator disk and be separated soft bag.When blood engorgement is separated soft bag, the lateral wall of interior cylinder can be close in the medial wall of the soft bag of described separation, the internal chamber wall of outer cylinder can be close in the lateral wall being separated soft bag, namely be separated in soft bag formed one with the separated space being separated lacuna and being close, the continuous centrifugal separation process of blood completes in this separated space.In separator disk, arrange the object being separated soft bag be repeatedly to use separator disk and single use to be separated soft bag, to avoid the pollution between different blood, and be convenient to improve separation efficiency and reduce separation costs.
The described soft bag of disposable separation is a kind of flat bag shaped structure with multiple liquid transmission pipeline, and as shown in Figure 4, Fig. 4 is the deployed configuration schematic diagram being separated soft bag in the present invention to its structure.The soft bag of described separation is by being separated bag and three pipelines being connected with bag form; Wherein, being separated bag is rectangular software plastic bag, and it makes the standard that material will meet medical plastic; The seamed edge on this long limit of separation bag is provided with three pipelines be communicated with inside and outside bag, lay respectively at be separated soft bag front portion, middle part, rear portion; Wherein, being arranged in front portion and stretching into the region at pipeline place being separated bag is the blood plasma district being separated lacuna, because the density of blood plasma is less, is in the nearest position of distance rotating shaft being separated in lacuna, therefore claim this pipeline to be plasma tube 4, for extracting isolated blood plasma from the soft bag of separation; Being arranged in middle part and stretching into the region at pipeline place being separated bag is the chaotic region being separated lacuna, for carrying whole blood in centrifugalize bag, therefore is called whole blood pipeline 5; Be positioned at the pipeline connection at rear portion and extend into separation bag, because the density of blood cell is larger, distance rotating shaft position is farthest in separation lacuna, the region at this pipeline place is the blood cell district be separated in lacuna, therefore claim this pipeline to be blood cell pipeline 6, for extracting isolated blood cell from the soft bag of separation.
When applying continuous centrifugal separation method of the present invention, blood enters into from the flexible pipe and blood inlet that are arranged in the middle part connection being separated soft bag and is separated soft bag, and the whole blood containing various blood constituent is full whole separated space gradually.In full process, be subject to the continuous action of centrifugal force, blood plasma is separated gradually with visible components such as blood cells, and in separated space, low centrifugal force district is proximally to the high centrifugal force field of far-end, is rendered as the distribution of the visible component segmentation and regionalizations such as blood plasma, whole blood and blood cell successively.Assemble the non-separating whole blood of major part in the middle part of separated space and be called chaotic region, the low centrifugal force region of separated space near-end is called blood plasma district, the high centrifugal force region of separated space far-end is called blood cell district.
As separator disk high speed rotating under the drive of flexible axle of centrifugalize container, the structure of its whole centrifugal separation system and operation logic are at the differential centrifugal separation system of a Chinese patent application cn201020293871.4(mixing material) in illustrate, as shown in Figure 2, repeat no more herein.Separator disk needs around the rotating shaft high speed rotating being positioned at center under the drive of flexible axle, for the centrifugalize of blood provides power.Flexible axle stretches into the rotary shaft position being connected and fixed on and being positioned at separator disk center, drive separation container rotates, described blood cell pipeline, whole blood pipeline are communicated with the tube for transfusion designed in flexible axle with plasma tube, thus require that flexible axle possesses the dual-use function of power transmission and liquid transfer.
On centrifugalize container, there is an intersection point central shaft of separator disk and separator disk bottom surface, and this intersection point is the junction point of flexible axle and separator disk, are also the power resources points that separator disk rotates.When the whole blood containing whole blood composition to enter from whole blood pipeline be separated in bag time, because the outboard sidewalls of this separation bag and the lacuna of separator disk fit formation separated space, namely can think that blood has entered in the separation lacuna of centrifugalize container.In the separated space of high speed rotating, owing to being subject to the continuous action of centrifugal force, enter into from whole blood pipeline the whole blood being separated bag to flow and accumulation along the medial wall being separated bag to the separated space far-end that centrifugal force is higher, near-end gradually to separated space after the far-end of this separated space is full of develops, as shown in Figure 5, in figure, the whole blood that is oriented to of arrow 7 enters the flow direction be separated in lacuna.
In the process that whole blood is piled up, be deposited in the whole blood of separated space distalmost end owing to being subject to the effect of centrifugal force comparatively greatly, the various compositions in whole blood start to be separated.Along with the continuous action of centrifugal force, blood plasma strengthens gradually with visible components such as blood cells and is separated, the visible components such as blood cell are piled up from separated space far-end to the blood inlet of centre after being separated, blood plasma is then piled up from separated space near-end to the blood inlet of centre, when these two kinds of deposits meet near blood inlet, there will be mixed interface, the whole blood simultaneously owing to constantly injecting impacts impact, the single component be separated herein mixes with whole blood, forms chaotic region.Now the state of blood in the soft bag of separation as shown in Figure 6, in figure, arrow 7 is separated lacuna later to the flow direction being separated lacuna far-end for whole blood enters, arrow 8 is after whole blood is accumulated to blood inlet, and the whole blood fraction entered is to being separated the dynamic direction of lacuna proximal flow.
When the input of whole blood is full of soft bag and the visible components such as blood plasma and blood cell realize being separated, there is the blood plasma meeting Plasma Pheresis/Apheresis Plasma standard in separated space near-end, can blood plasma be extracted out from separated space near-end respectively and extract the visible components such as blood cell out from separated space far-end, inject whole blood at blood inlet simultaneously, whole blood input quantity is the summation of blood cell and blood plasma extracted amount, thus the cumulative volume maintaining liquid in whole separated space is constant, realizes dynamic equilibrium.Blood and the various compositions separated thereof the state now in separated space as shown in Figure 7, in figure, arrow 7 and arrow 8 are respectively whole blood and enter to the direction being separated the far and near two ends flowings of lacuna when being separated soft bag, and arrow 9 is positioned at the flow direction when blood plasma being separated lacuna near-end is extracted out by plasma tube 4.
Method of the present invention be a kind of blood continuous centrifugal be separated optimization method, whole blood input and blood plasma and blood cell output procedure dynamic equilibrium in, the blood in separated space receives many-sided active force.One, blood itself is non-newtonian liquid, blood plasma in blood and visible component have significant viscosity, the negative pressure that the malleation of whole blood input and blood plasma blood cell extract has impelled the whole blood flow in separated space, and the lateral wall of separated space, medial wall produce frictional force to the flowing of whole blood, stop whole blood flow, the closer to sidewall, flow velocity is slower, produces current gradient lamination; They are two years old, by the continuous action of centrifugal force, the visible component such as the blood cell that whole blood Midst density is larger trend is distributed in far-end and the lateral wall of separated space, thus is rendered as the segmentation distribution of the visible components such as blood plasma, whole blood and blood cell successively to the high centrifugal force field of far-end by the low centrifugal force district of near-end in separated space; Its three, the impact of gravitate, the bottom that the visible components such as the blood cell that the density in separated space is larger trend towards being deposited in separated space is formed assembles line, makes these visible components closer to blood plasma mouth.
For this reason, when blood plasma is extracted out by plasma tube fast in blood plasma district, blood plasma axial flow closes on the visible components such as the blood cell of separating interface and produces drag force to lateral wall and bottom, impel the bottom of these visible components against the component effect of centrifugal force along separated space to spread along blood plasma axial flow direction, diffuse into even blood plasma mouth and affect the plasma purity of collection.The principle of optimality of the present invention is as shown in Figure 8,9: when curved body container is at high speed rotating, be separated lacuna outer wall 20 and produce two power by visible components such as the blood cells 19 in soft bag sidewall 21 pairs of separated spaces, one is centripetal force 15, limit is pointed in direction, another is frictional force 13, and direction is the tangential direction of direction of rotation.When the direction of rotation 10 of curved body container is contrary with the expansion direction of described curve, drag force 14 action direction of the visible components such as the 11 pairs of blood cells in frictional force 13 direction and blood plasma axial flow is identical, the visible components such as blood cell can be accelerated spread to blood plasma mouth, adding gravity 12 factor can cause blood cell to separated space flows, make blood cell be easy to be mixed into plasma flow closer to blood plasma mouth, and then have influence on the disintegrate-quality of blood plasma.But, as shown in Figure 10 and Figure 11, when the direction of rotation 16 of curved body container is identical with the expansion direction of described curve, drag force 14 action direction of the visible components such as the 11 pairs of blood cells 19 in frictional force 17 direction and blood plasma axial flow is contrary, can suppress to spread to blood plasma mouth because gravity 12 is deposited in the visible components such as blood cell bottom separated space.When not increasing separation costs by controlling direction of rotation like this, can improve disintegrate-quality, therefore the direction of rotation of described curved body container is identical with the expansion direction of described curve has more optimization.
Embodiment 1
In the present embodiment, centrifugalize container constitutes separation lacuna by continuous curve surface body, the lateral wall curved surface of this separation lacuna perpendicular to the curve that the plane of rotating shaft projects is being:
Wherein, polar limit O is the intersection point of rotating shaft and described plane, and polar pole axis L is the ray that limit arrives curve initiating terminal direction, and the positive direction of polar angle is that r is the footpath, pole of any point on curve, R=199mm, θ clockwise
1=0 degree, θ
2=90 degree, θ
3=255 degree, θ
4=380 degree; In described separation lacuna, [0
o, 90
o) be blood plasma district, [90
o, 255
o) be chaotic region, [255
o, 380
o] be blood cell district, b
1=0.8, b
2=0.4, b
3=0.2.The direction of rotation of this separation container is identical with the expansion direction being separated lacuna.
After whole blood is full of whole separation lacuna, whole blood continues input from blood inlet, and blood plasma continues the visible components such as extraction and blood cell and continues to extract out from blood cell mouth from blood plasma mouth, input quantity is equal with discharge, make total liquid volume in separation lacuna keep balance, thus reach the object being continuously separated blood.
Certainly, the method that the present invention is separated blood continuous centrifugal, except the structure composition enumerated in above-described embodiment and curve form, can be made up of and curve form other similar structures in addition.Generally speaking, protection scope of the present invention also comprises other conversion that it will be apparent to those skilled in the art that and substitutes.
Claims (2)
1. the blood optimization method that continuous centrifugal is separated in curved body container, it is characterized in that, separation lacuna is provided with in described curved body container, this curved body container, around rotating shaft high speed rotating, utilizes centrifugal force that the blood be injected in described separation lacuna is gone out heterogeneity by density size separation; Described separation lacuna is the curved body including outer wall and inwall, and the outer wall curved surface of this separation lacuna is as follows with polar coordinate representation perpendicular to the curve that the plane of rotating shaft projects:
Wherein, polar limit O is the intersection point of rotating shaft and described plane, and polar pole axis L is the ray that limit arrives curve initiating terminal direction, the positive direction of polar angle is clockwise, r is the footpath, pole of any point on curve, and R is the footpath, pole of curve distalmost end, θ
1for the polar angle of curve initiating terminal, its value is 0 degree of polar coordinate system, θ
2for curve and blood plasma separating factor critical radius F
rthe polar angle of intersection point, blood plasma separating factor critical radius F
rfor blood plasma in unit interval when rotating speed is determined is separated required minimum centrifugal radius, θ
3be a polar angle, its value is θ
21.5 ~ 3.5 times, θ
4for the polar angle of curve distalmost end; In described separation lacuna, [θ
1, θ
2) be blood plasma district, [θ
2, θ
3) be chaotic region, [θ
3, θ
4] be blood cell district, curve coefficients refers to the rate of change of footpath, pole relative to polar angle of any point on curve, b
1for the described curve coefficients in blood plasma district, b
2for the described curve coefficients of chaotic region, b
3for the described curve coefficients in blood cell district; The direction of rotation of described separation container is identical with the expansion direction being separated lacuna; The expansion direction of described separation lacuna refers to: in the blood plasma district of described curve, have any two points p and q, and its polar angle is respectively θ p and θ q, and corresponding footpath, pole is respectively rp and rq, and θ
1≤ θ p < θ q < θ
2, rp<rq simultaneously, some p are curve expansion direction along curved trajectory to the direction of some q.
2. a kind of blood according to claim 1 optimization method that continuous centrifugal is separated in curved body container, it is characterized in that, the separation process of described blood continuous centrifugal specifically comprises the steps:
The first step, the curved body container described in high speed rotating, is placed in the soft bag of the separation high speed rotating be thereupon separated in lacuna, and the front portion of the soft bag of this separation, middle part, rear portion are respectively equipped with the flexible pipe that is carried liquid;
Second step, blood is injected into the soft bag of separation by being positioned at the flexible pipe be separated in the middle part of soft bag, be full of behind the soft bag space of separation until whole blood, be separated soft bag and be separated lacuna and fit tightly the inner separation space formed with being separated lacuna and being close, blood in the soft bag of separation by various composition segmentation and regionalization gradually under centrifugal force continuous action, blood plasma is gathered in gradually and is separated soft bag front portion and forms a blood plasma district, the visible component comprising blood cell is gathered in gradually and is separated soft bag rear portion and forms a blood cell district, and be separated lasting input formation chaotic region due to whole blood, middle part of soft bag,
3rd step, blood plasma is extracted out in blood plasma district from being arranged on the flexible pipe being separated soft bag front portion, the visible component comprising blood cell is extracted out in blood cell district from being arranged on the flexible pipe being separated soft bag rear portion, continue input whole blood from being arranged on the flexible pipe be separated in the middle part of soft bag simultaneously, reach with extraction the dynamic equilibrium being separated soft bag inner capacities by control inputs, and then the continuous centrifugal realizing blood is separated.
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| DE4226974C2 (en) * | 1992-08-14 | 1994-08-11 | Fresenius Ag | Method and device for the continuous preparation of a cell suspension |
| WO2002062482A2 (en) * | 2000-11-02 | 2002-08-15 | Gambro, Inc. | Fluid separation devices, systems and methods |
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| US4419089A (en) * | 1977-07-19 | 1983-12-06 | The United States Of America As Represented By The Department Of Health And Human Services | Blood cell separator |
| US4387848A (en) * | 1977-10-03 | 1983-06-14 | International Business Machines Corporation | Centrifuge assembly |
| US6277060B1 (en) * | 1998-09-12 | 2001-08-21 | Fresenius Ag | Centrifuge chamber for a cell separator having a spiral separation chamber |
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