CN102698321A - Preparation method of bone repairing body with drug loading function - Google Patents
Preparation method of bone repairing body with drug loading function Download PDFInfo
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- CN102698321A CN102698321A CN2012102205469A CN201210220546A CN102698321A CN 102698321 A CN102698321 A CN 102698321A CN 2012102205469 A CN2012102205469 A CN 2012102205469A CN 201210220546 A CN201210220546 A CN 201210220546A CN 102698321 A CN102698321 A CN 102698321A
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Abstract
The invention discloses a preparation method of a bone repairing body with drug loading function. The preparation method comprises the following steps: firstly, dissolving acetone in poly lactic acid (PLA), adding de-ionized water in tertiary calcium phosphate (TCP) to obtain a slurry; then mixing the de-ionized water and the TCP and adding drugs into the mixture; subsequently, preparing the bone repairing body by adopting a low-temperature shaping technique, placing the bone repairing body in a freeze-drying machine to pre-freeze; and finally, drying the bone repairing body through the freeze-drying technique. The bone repairing body prepared by the method provided by the invention can satisfy various performance requirements of planting brackets as well as load drugs, so that the problem of effectively loading the bone bracket materials and the growth factors in the bone organization engineering is solved; the degradation speeds of different components in the obtained bone repairing body are different, the bone repairing body is reserved with ducts in the process of degrading so as to release drugs gradually, better induce and accelerate the bone repairing.
Description
Technical field
The present invention relates to a kind of method for preparing of bone repair, particularly a kind of method for preparing of the drug release body based on low-temperature extrusion forming is in the bone tissue engineer field extensive use of repairing bone defect.
Background technology
Treating bone at present clinically damaged is main mode with autologous bone transplanting still, but still exists some problems.Application bone tissue engineer technology prepares artificial dummy and comes the research of repairing bone defect very extensive, is considered to one of most effectual way of carrying out future the bone reparation.
Bone tissue engineering stent material is one of key issue of bone tissue engineer research.Ideal bone holder material need have excellent biological compatibility, degradability, and three-dimensional inline loose structure, certain mechanical strength, it is moulding to be prone to processing.The stock that is applied in the bone tissue engineer mainly comprises ceramic material, natural and synthetic macromolecular material and composite.The single type material generally is difficult to satisfy the requirement of bone tissue engineer with the extracellular timbering material; Through certain method that several kinds of homogenous materials are compound; Comprehensive various material pluses and minuses; Form compound timbering material, improve degradation property, biocompatibility and the mechanical strength of material, more and more general.PLA is the earliest as bone and cartilage tissue engineered timbering material; Be to use engineering material of bone tissue widely at present, degradation in vivo generates carbohydrate metabolism product lactic acid polylactic acid and has excellent biological compatibility, biological degradability and bioresorbable and mechanical performance.TCP is a kind of of Ca-P ceramic; With people's osseous tissue inorganic structure and form similar; Have excellent synosteosis property and bone conduction performance, its calcium-phosphorus ratio is 1.50, and two kinds of crystal formations of α and β are arranged; Medically using more mainly is low temperature phase bata-tricalcium phosphate (β-tricalciumphosphate, β-TCP).The biodegradation of this material is very fast, can be substituted by area of new bone after the absorption, generates osteoblast.
Somatomedin is one type and is present in intravital bioactie agent, also is one of key issue of bone tissue engineer research, can regulate cell growth, tissue regeneration and wound healing, only by suitable carriers somatomedin competence exertion optimum utility.General medicine load mode is that support is immersed in the drug solvent; The prominent of medicine appears in the bone repair of this method preparation easily releases; And because medicine is mixed uniformly; The bone repair of this method preparation is prone to cause in good time or too early not running off of medicine, is difficult to satisfy bone and repairs the different demands of different times to somatomedin.
Summary of the invention
In order to solve the prior art problem, the objective of the invention is to overcome the defective that prior art exists, a kind of bone repair method for preparing with medicine load function is provided; Adopt each component of first mixing to carry out stent forming method again, can obtain bone repair, can not only satisfy each item performance requirement of plantation support; Can also drug loading; Solved the efficient loading problem of bone holder material and somatomedin in the bone tissue engineer, the different component degradation speed in the bone repair that is obtained is different, and degradation process makes the bone repair reserving hole channel; Discharge medicine gradually, better induce and promote the skeletonization reparation.
For reaching the foregoing invention purpose, the present invention adopts following technical proposals:
A kind of bone repair method for preparing with medicine load function may further comprise the steps:
(1) acetone soln of PLA preparation: adopt the solvent of acetone, make PLA be dissolved in acetone solvent fully, obtain the acetone soln of PLA as PLA;
(2) TCP pulp preparation: with adding deionized water in the TCP powder, full and uniform mixing obtains the TCP slurry;
(3) drug release composite material slurry preparation: the acetone soln of the PLA that will in above-mentioned steps (1), obtain, TCP slurry and the medicine that in above-mentioned steps (2), obtains fully mix, and obtain the drug release composite material serosity of compounding ingredients;
(4) bone repair cryogenic forming: the drug release composite material serosity that will in above-mentioned steps (3), obtain is injected into the bone repair that is shaped in the low temperature moulding machine;
(5) bone repair lyophilization: selection is inserted precooling in the freezer dryer through the good dummy of shaping that above-mentioned steps (4) obtains with it, and evacuation is dry then, finally obtains the bone repair finished product.
In above-mentioned steps of the present invention (2), at first preferably the TCP material is carried out physical grinding, and then, obtain being used to prepare the TCP powder of TCP slurry through sieving greater than 150 purpose sieve apertures.
In above-mentioned steps of the present invention (3), the solids content of drug release composite material serosity is preferably 40%.
The present invention has following conspicuous outstanding substantive distinguishing features and remarkable advantage compared with prior art:
1. the different component degradation speed in the bone repair of the inventive method preparation is different, and the PLA degraded is fast than TCP, and degradation process makes the bone repair reserving hole channel, discharges medicine gradually, better induces and promotes the skeletonization reparation;
2. the present invention adopts the combined process of lyophilization and quick shaping, can satisfy the intensity and the shape requirement of the plantation support that in the bone tissue engineer of repairing bone defect, uses, and can avoid the pyroprocess of medicine again;
3. the present invention adopts the solvent of acetone as PLA, and acetone is fully volatilized, and avoids toxic and side effects, improves the safety in the bone repair use;
4. the invention solves the efficient loading problem of bone holder material and somatomedin in the bone tissue engineer; Make somatomedin repair different times at bone and all can bring into play optimum utility, the bone repair for preparing through the inventive method can satisfy the different demands of bone reparation different times to somatomedin.
The specific embodiment
Details are as follows to the preferred embodiments of the present invention:
A kind of bone repair method for preparing with medicine load function may further comprise the steps:
(1) acetone soln of PLA preparation: adopt the solvent of acetone as PLA; Make PLA be dissolved in acetone solvent fully; Obtain the acetone soln of PLA, adopt the solvent of acetone, can acetone fully volatilized as PLA; Avoid toxic and side effects, improve the safety in the bone repair use;
(2) TCP pulp preparation: the TCP material is put into grinder carry out physical grinding; Through sieving greater than 150 purpose sieve apertures, obtain being used to prepare the TCP powder of TCP slurry then, this TCP powder has the powder size effect of TCP micropowder; Be easy in liquid, realize disperseing; With adding deionized water in the TCP powder, full and uniform mixing obtains the TCP slurry then;
(3) drug release composite material slurry preparation: the acetone soln of the PLA that will in above-mentioned steps (1), obtain, TCP slurry and the medicine that in above-mentioned steps (2), obtains fully mix; Obtain solids content and be the drug release composite material serosity of 40% compounding ingredients; Can obtain having the bone repair that the composite component is optimized proportioning more, and satisfy the requirement to material of follow-up stent forming technology.
(4) bone repair cryogenic forming: the drug release composite material serosity that will in above-mentioned steps (3), obtain is injected into the bone repair that is shaped in the low temperature moulding machine; Adopt the low temperature RP technique; Can prepare bone repair support with medicine carrying function; The intensity and the shape requirement of the plantation support that in the bone tissue engineer of repairing bone defect, uses can be satisfied, the pyroprocess of medicine can be avoided again;
(5) bone repair lyophilization: selection is inserted precooling in the freezer dryer through the good dummy of shaping that above-mentioned steps (4) obtains with it, and evacuation is dry then; Finally obtain the bone repair finished product, the PLA degraded in the bone repair of present embodiment preparation is fast than TCP, and degradation process makes the bone repair reserving hole channel; Discharge medicine gradually; The prominent of medicine can not taken place release, can not cause in good time or too early not running off of medicine yet, can induce and promote the skeletonization reparation better.
Above the embodiment of the invention is illustrated; But the invention is not restricted to the foregoing description; Can also make multiple variation according to the purpose of innovation and creation of the present invention; Change, the modification of making under all spirit and principles according to technical scheme of the present invention, substitute, combination, simplify, all should be the substitute mode of equivalence, as long as goal of the invention according to the invention; Only otherwise deviate from know-why and the inventive concept that the present invention has the bone repair method for preparing of medicine load function, all belong to protection scope of the present invention.
Claims (3)
1. the bone repair method for preparing with medicine load function is characterized in that, may further comprise the steps:
(1) acetone soln of PLA preparation: adopt the solvent of acetone, make PLA be dissolved in acetone solvent fully, obtain the acetone soln of PLA as PLA;
(2) TCP pulp preparation: with adding deionized water in the TCP powder, full and uniform mixing obtains the TCP slurry;
(3) drug release composite material slurry preparation: the acetone soln of the PLA that will in above-mentioned steps (1), obtain, TCP slurry and the medicine that in above-mentioned steps (2), obtains fully mix, and obtain the drug release composite material serosity of compounding ingredients;
(4) bone repair cryogenic forming: the drug release composite material serosity that will in above-mentioned steps (3), obtain is injected into the bone repair that is shaped in the low temperature moulding machine;
(5) bone repair lyophilization: selection is inserted precooling in the freezer dryer through the good dummy of shaping that above-mentioned steps (4) obtains with it, and evacuation is dry then, finally obtains the bone repair finished product.
2. the bone repair method for preparing with medicine load function according to claim 1; It is characterized in that: in above-mentioned steps (2); At first the TCP material is carried out physical grinding, through sieving, obtain being used to prepare the TCP powder of TCP slurry then greater than 150 purpose sieve apertures.
3. the bone repair method for preparing with medicine load function according to claim 1 and 2 is characterized in that: in above-mentioned steps (3), the solids content of drug release composite material serosity is 40%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103638559A (en) * | 2013-12-16 | 2014-03-19 | 武汉纺织大学 | Water-insoluble ultrafine fibroin powder/polylactic acid composite porous scaffold material and preparation method thereof |
| CN103908702A (en) * | 2014-03-10 | 2014-07-09 | 上海大学 | Preparation method of nano material-based bionic drug-loaded gradient slow release bone scaffold |
| CN103908697A (en) * | 2014-03-10 | 2014-07-09 | 上海大学 | Preparation method of nano material-based bionic bone scaffold |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070098756A1 (en) * | 2005-11-01 | 2007-05-03 | Keyvan Behnam | Bone Matrix Compositions and Methods |
| CN101293116A (en) * | 2008-06-12 | 2008-10-29 | 郑隆泗 | Absorbent bone interface screw nail and preparation method thereof |
| CN101721747A (en) * | 2008-10-27 | 2010-06-09 | 香港中文大学 | Biocomposite material, preparing method and use thereof |
-
2012
- 2012-06-29 CN CN2012102205469A patent/CN102698321A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070098756A1 (en) * | 2005-11-01 | 2007-05-03 | Keyvan Behnam | Bone Matrix Compositions and Methods |
| CN101293116A (en) * | 2008-06-12 | 2008-10-29 | 郑隆泗 | Absorbent bone interface screw nail and preparation method thereof |
| CN101721747A (en) * | 2008-10-27 | 2010-06-09 | 香港中文大学 | Biocomposite material, preparing method and use thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103638559A (en) * | 2013-12-16 | 2014-03-19 | 武汉纺织大学 | Water-insoluble ultrafine fibroin powder/polylactic acid composite porous scaffold material and preparation method thereof |
| CN103908702A (en) * | 2014-03-10 | 2014-07-09 | 上海大学 | Preparation method of nano material-based bionic drug-loaded gradient slow release bone scaffold |
| CN103908697A (en) * | 2014-03-10 | 2014-07-09 | 上海大学 | Preparation method of nano material-based bionic bone scaffold |
| CN103908702B (en) * | 2014-03-10 | 2015-08-12 | 上海大学 | A kind of preparation method based on nano material bionical medicine carrying gradient slow release bone support |
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Application publication date: 20121003 |