CN102393450A - Magnetic tweezers probe based on optical fiber - Google Patents
Magnetic tweezers probe based on optical fiber Download PDFInfo
- Publication number
- CN102393450A CN102393450A CN2011102208890A CN201110220889A CN102393450A CN 102393450 A CN102393450 A CN 102393450A CN 2011102208890 A CN2011102208890 A CN 2011102208890A CN 201110220889 A CN201110220889 A CN 201110220889A CN 102393450 A CN102393450 A CN 102393450A
- Authority
- CN
- China
- Prior art keywords
- optical fiber
- magnetic
- layer
- probe based
- probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention discloses a magnetic tweezers probe based on an optical fiber. The magnetic tweezers probe is characterized in that: a probe core is constructed by an optical fiber of which the surface is provided with a magnetic core layer; light beams transmitted by the optical fiber can be emitted from the front end face of the probe core; and the periphery of the probe core is provided with an inner heat conducting insulating layer, a micro spiral coil, an outer heat conducting insulating layer and a shield layer from inside to outside in sequence. The magnetic tweezers probe has the advantages of small size, no need of any complex radiating structure, adjustable size and direction of a generated magnetic field, flexible movement and realization of the functions of capturing, controlling and optically operating biomolecules.
Description
Technical field
The present invention relates to a kind of magnetic tweezer probe, particularly a kind of magnetic tweezer probe based on optical fiber.
Background technology
The magnetic tweezer is to utilize externally-applied magnetic field control micron or nano-scale magnetic-particle to carry out little technology of controlling; Since 1949, be introduced into biology field [Crick F.H.C first by people such as Crick F.H.C; Hughes A.F.W; The physical properties of cytoplasm:a study by means of the magnetic particle method, Exp.Cell.Res.1:37-80,1949].Adopt the magnetic tweezer that biomacromolecule is carried out biology and control and to study the cell internal characteristic, confirm the individual cells mechanical property, separate the important application such as cell that adopt the magnetic ball mark in batches.Because magnetic tweezer pair cell and biomacromolecule operating process can not cause that damage forms to biology and control one of major technique.Control compared with techniques with light tweezer, AFM and microscopic capillary probe equimolecular, the magnetic tweezer has noncontact, little to biological sample damage, can apply advantage such as stable, sizeable power and be widely used in biology field.
The basic step that the magnetic tweezer is controlled be through the dna molecular of biochemical modification or other biomacromolecule (promptly dna molecular or other biomacromolecule two terminal modified on the different functions base) end be fixed on the magnetic ball or substrate that is modified with antibody; Can produce field pole or seizure of magnetic tweezer probe and control magnetic ball through adding, thereby the realization molecule is controlled.In this course, the design of the magnetic pole of magnetic tweezer or magnetic tweezer probe is the key that realizes that biomolecule is captured and controlled.Magnetic tweezer magnetic pole has the single magnetic pole of employing permanent magnet making or the magnetic field of many structure of magnetic pole to realize the control [S.B.Smith to biomolecule; L.Finzi; C.Bustamante, Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads, Science 258; 1122,1992].Abroad the someone adopts thick conductive coil after-combustion to realize the [F.Zienmann that controls to biomolecule at the magnetic pole of soft magnetic core or the making of iron cobalt core; J.Radler; E.Sackmann; Local measurements of viscoelastic moduli of entangled actin networks using an oscillating magnetic bead micro-rheometer, Biophys.J.66.2210,1994; Charbel Haber, Denis Wirtz, Magnetic tweezers for DNA micromanipulation, Review of Scientific Instruments, J.71,4561,2000; H.B.Anthony de Vries, Bea E.Krenn, Roel van Driel, Johhannes S.Kanger, Micro magnetic tweezers for nanomanipulation inside live cells, Biophys.J.88,2137,2005].But the magnetic pole that this type magnetic tweezer uses has been owing to adopted the permanent magnet or the thick line circle of bulky, has that volume is big, power consumption is big, and magnetic pole can't move flexibly, and hot-wire coil involves great expense for producing the radiator structure that enough big magnetic field needs complicacy simultaneously.In order to overcome these deficiencies; People such as Chi-Han Chiou have proposed to utilize dull and stereotyped little coil to make many magnetic poles and have realized [Chi-Han Chiou, Gwo-Bin Lee, the A micromachined DNA manipulation platform for the stretching and rotation of a single DNA molecule of controlling to biomolecule; J.Micromech.Microeng.; 15,109,2005; Chi-Han Chiou, Yu-Yen Huang, Meng-Han Chiang; Huei-Huang Lee and Gwo-Bin Lee; New magnetic tweezers for investigation of the mechanical properties of single DNA molecules, Nanotechnology, 17; 1217,2006].Though the magnetic tweezer probe that utilizes dull and stereotyped little coil to make has been realized microminiaturization, the coil after-combustion number of turns is few, and generation magnetic field is not strong, and probe can't move, and is not good enough to the dirigibility that biomolecule is captured and controlled.
Summary of the invention
The present invention is for avoiding above-mentioned existing in prior technology weak point, provide a kind of volume little, need not complicated radiator structure, can produce the magnetic field size and Orientation adjustable, can move flexibly and realize capturing, controlling and the magnetic tweezer probe based on optical fiber of optical manipulation function biomolecule.
The present invention adopts following technical scheme for the technical solution problem:
The design feature that the present invention is based on the magnetic tweezer probe of optical fiber is the optical fiber formation nook closing member that has core layer with the surface, can be from the front end face ejaculation of nook closing member through the light beam of Optical Fiber Transmission; Heat conductive insulating layer, little spiral winding in the periphery of said nook closing member sets gradually from inside to outside, outer heat conductive insulating layer and screen layer.
The design feature that the present invention is based on the magnetic tweezer probe of optical fiber also is:
Said optical fiber is single-core fiber or multi-core fiber.
The leading portion of said nook closing member is set to be the probe of pencil head, through the front end face outgoing from probe of the light beam of Optical Fiber Transmission.
The front end face of said probe is plane surface or is spherical crown surface.
The core layer of said optical fiber surface is to adopt the magnetic material thin layer of vacuum coating or electron beam plated film or magnetron sputtering film production.
The heat conductive insulating layer is the heat conducting insulating film layer that adopts heat-conducting insulation material to make in said.
Said little spiral winding is that the conductive membrane layer produced by micro processing that adopts vacuum coating or electron beam plated film or magnetron sputtering plating to form is formed.
Said outer heat conductive insulating layer is the heat conducting insulating film layer that adopts heat-conducting insulation material to make.
Said screen layer is the metal film layer that adopts vacuum coating or electron beam plated film or magnetron sputtering film production.
Said little spiral winding is a single layer coil or lattice coil overlaying structure from inside to outside.
Compared with present technology, beneficial effect of the present invention is embodied in:
1, the present invention is owing to adopt little spiral winding, and volume is little, can move flexibly, and probe has the size close with biomolecule, can realize capturing and controlling biomolecule.
2, magnetic tweezer probe of the present invention can produce enough strong magnetic field, and can realize the closely operation to biomolecule, also can change the size and Orientation of operating physical force through regulating the size and Orientation of little spiral winding electric current.
3, the present invention utilizes optical fiber that laser is conducted to the probe outgoing, can realize the optical manipulation to the biomolecule of capturing.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Fig. 2 is optical fiber synoptic diagram among the present invention.
Label among the figure: 1 optical fiber; 2 core layer; Heat conductive insulating layer in 3; 4 little spiral windings; 5 outer heat conductive insulating layers; 6 screen layers.
Embodiment
Referring to Fig. 1, Fig. 2, present embodiment is the optical fiber 1 formation nook closing member that has core layer 2 with the surface, can be from the front end face ejaculation of nook closing member through the light beam of Optical Fiber Transmission; Heat conductive insulating layer 3, little spiral winding 4 in the periphery of nook closing member sets gradually from inside to outside, outer heat conductive insulating layer 5 and screen layer 6.
In the practical implementation, the corresponding structure setting also comprises:
Optical fiber 1 is single-core fiber or multi-core fiber, and single-core fiber can realize that laser beam by the output of nook closing member front end face collimation, carries out optical manipulation to biomolecule; Also can adopt multi-core fiber,, laser through the output of two fiber cores, can be formed light tweezer acting force to biomolecule, constitute the compound bio molecule manipulation with the effect of magnetic tweezer like the symmetric double core fibre;
The leading portion of nook closing member is set to be the probe of pencil head; Because the magnetic material that probe covers; The tip of the probe of pencil head can strengthen the magnetic field intensity that spiral winding produces; Biomolecule is formed enough big magnetic force, through the front end face outgoing of the light beam of Optical Fiber Transmission from probe;
The front end face of probe is plane surface or is spherical crown surface that plane surface can directly be derived light beam, form the fluorescent illumination of quilt operation biomolecule, and spherical crown surface can form the effect of converging to output beam by similar lens, and biomolecule is formed light tweezer operation light field;
The core layer 2 of optical fiber surface is to adopt the magnetic material thin layer of vacuum coating or electron beam plated film or magnetron sputtering film production;
Interior heat conductive insulating layer 3 is the heat conducting insulating film layers that adopt heat-conducting insulation material to make;
Little spiral winding 4 is that the conductive membrane layer produced by micro processing that adopts vacuum coating or electron beam plated film or magnetron sputtering plating to form is formed;
Outer heat conductive insulating layer 5 is the heat conducting insulating film layers that adopt heat-conducting insulation material to make;
Screen layer 6 is the metal film layers that adopt vacuum coating or electron beam plated film or magnetron sputtering film production;
Little spiral winding 4 is single layer coil or lattice coil overlaying structures from inside to outside, and the increase of the coil number of plies can be strengthened the total magnetic field that hot-wire coil produces, and under the certain condition of magnetic operating physical force, electrical current can be lower, reduces coil heating and power consumption.
Manufacturing process:
1, the processing of optical fiber: get one section single core glass optical fiber, its surface coating layer is divested,, dry for use with alcohol sonic oscillation clean surface;
2, magnetic tweezer probe is handled: utilize the optical fiber end grinding system to carry out attrition process at optical fiber one end and become cone as shown in Figure 2; The cone top is the disk of diameter 10-20 micron; Semi-cone angle is about 15 °-20 °, and the optical fiber after grinding is carried out ultrasonic cleaning and oven dry;
3, core layer and interior heat conductive insulating layer are made: optical fiber is put into vacuum coating equipment, vacuumize and reach plated film requirement 10-3Pa, target is a pure iron; With the rotating machinery device optical fiber is at the uniform velocity rotated; The control plated film time obtains surface plating soft iron optical fiber, and thickness of coating is the 10-15 micron; Optical fiber is taken out, adopt epotek-930 heat conductive insulating to solidify the encapsulation of glue press mold on its surface, thickness is the 30-50 micron, in high temperature oven, is provided with 150 ℃ and solidifies 15 minutes, wait to solidify accomplish the back take out cool off for use;
4, little spiral winding is made: the optical fiber after step 3 is handled adopts the film plating process identical with step 3 to carry out plated film, and Coating Materials is a fine silver, and thickness is the 50-60 micron; Employing standard photoetching micro fabrication carries out little processing to silver layer; Finally produce the silver-colored coil of helical structure; Wire circle is 100, and coil width is the 50-55 micron, and coil pitch is the 30-45 micron; And be 120 microns enamel-cover copper conductors welding with coil two ends and diameter, produce the lead-in wire that can link to each other with extraneous power supply.
5, outer heat conductive insulating layer and screen layer are made: outside little helix ring layer, be coated with the heat conductive insulating layer, thickness is the 30-50 micron; Put it into vacuum coating equipment, plating layer of metal copper film, thickness is the 10-20 micron.
Claims (10)
1. the magnetic tweezer probe based on optical fiber is characterized in that the optical fiber (1) that has core layer (2) with the surface constitutes nook closing member, can be from the front end face ejaculation of nook closing member through the light beam of Optical Fiber Transmission; Heat conductive insulating layer (3), little spiral winding (4), outer heat conductive insulating layer (5) and screen layer (6) in the periphery of said nook closing member sets gradually from inside to outside.
2. the magnetic tweezer probe based on optical fiber according to claim 1 is characterized in that said optical fiber is single-core fiber or multi-core fiber.
3. the magnetic tweezer probe based on optical fiber according to claim 1 is characterized in that the leading portion of said nook closing member is set to be the probe of pencil head, through the front end face outgoing from probe of the light beam of Optical Fiber Transmission.
4. the magnetic tweezer probe based on optical fiber according to claim 3, the front end face that it is characterized in that said probe are plane surface or are spherical crown surface.
5. the magnetic tweezer probe based on optical fiber according to claim 1, the core layer (2) that it is characterized in that said optical fiber surface are the magnetic material thin layers that adopts vacuum coating or electron beam plated film or magnetron sputtering film production.
6. the magnetic tweezer probe based on optical fiber according to claim 1 is characterized in that said interior heat conductive insulating layer (3) is the heat conducting insulating film layer that adopts heat-conducting insulation material to make.
7. the magnetic tweezer probe based on optical fiber according to claim 1 is characterized in that said little spiral winding (4) is that the conductive membrane layer produced by micro processing that adopts vacuum coating or electron beam plated film or magnetron sputtering plating to form is formed.
8. the magnetic tweezer probe based on optical fiber according to claim 1 is characterized in that said outer heat conductive insulating layer (5) is the heat conducting insulating film layer that adopts heat-conducting insulation material to make.
9. the magnetic tweezer probe based on optical fiber according to claim 1 is characterized in that said screen layer (6) is the metal film layer that adopts vacuum coating or electron beam plated film or magnetron sputtering film production.
10. the magnetic tweezer probe based on optical fiber according to claim 1 is characterized in that said little spiral winding (4) is a single layer coil or lattice coil overlaying structure from inside to outside.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110220889.0A CN102393450B (en) | 2011-08-03 | 2011-08-03 | Magnetic tweezers probe based on optical fiber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110220889.0A CN102393450B (en) | 2011-08-03 | 2011-08-03 | Magnetic tweezers probe based on optical fiber |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102393450A true CN102393450A (en) | 2012-03-28 |
| CN102393450B CN102393450B (en) | 2014-01-29 |
Family
ID=45860809
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110220889.0A Expired - Fee Related CN102393450B (en) | 2011-08-03 | 2011-08-03 | Magnetic tweezers probe based on optical fiber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102393450B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113144426A (en) * | 2020-01-07 | 2021-07-23 | 天津工业大学 | Improved micro magnetic coil with cell-level F/C structure |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0649670A (en) * | 1992-08-04 | 1994-02-22 | Canon Inc | Production of spiral member |
| US6675033B1 (en) * | 1999-04-15 | 2004-01-06 | Johns Hopkins University School Of Medicine | Magnetic resonance imaging guidewire probe |
-
2011
- 2011-08-03 CN CN201110220889.0A patent/CN102393450B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0649670A (en) * | 1992-08-04 | 1994-02-22 | Canon Inc | Production of spiral member |
| US6675033B1 (en) * | 1999-04-15 | 2004-01-06 | Johns Hopkins University School Of Medicine | Magnetic resonance imaging guidewire probe |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113144426A (en) * | 2020-01-07 | 2021-07-23 | 天津工业大学 | Improved micro magnetic coil with cell-level F/C structure |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102393450B (en) | 2014-01-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100498394C (en) | Double core single optical fiber optical tweezers for capturing minute particle and its manufacture method | |
| CN104003353B (en) | The preparation method of metal not close arrangement spherical nanoparticle array | |
| CN105820796A (en) | Preparation method of magnetic-alloy-loaded porous carbon sphere composite wave-absorbing material | |
| CN106772990B (en) | A kind of light control techniques for realizing the adjustment of cell tandem using double optical fiber optical tweezers | |
| US20180120345A1 (en) | Metallic device for scanning near-field optical microscopy and spectroscopy and method for manufacturing same | |
| WO2006084123A2 (en) | Self-assembly method, opal, photonic band gap, and light source | |
| CN106622436B (en) | Material distributed AC servo system platform and control method based on light stream whirlpool array | |
| Wang et al. | Direct blow spinning of flexible and transparent Ag nanofiber heater | |
| Yuan et al. | Invited Review Article: Tip modification methods for tip-enhanced Raman spectroscopy (TERS) and colloidal probe technique: A 10 year update (2006-2016) review | |
| CN102393450B (en) | Magnetic tweezers probe based on optical fiber | |
| CN104009137A (en) | High-speed directional-transmission single-photon-source device | |
| CN102658076A (en) | Micro-nanometer material as well as preparation method, device and application thereof | |
| CN109813349A (en) | A kind of composite optical fiber device and preparation and application detecting light, electricity and chemical signal | |
| CN110057751B (en) | Apparatus and method for fabricating optical particle probe | |
| Huang et al. | Photon momentum dictates the shape of swarming gold nanoparticles in optical trapping at an interface | |
| CN106248999B (en) | A kind of preparation method for the golden disk time micron electrode that geometry is controllable | |
| CN104761154B (en) | A kind of method that utilization organic macromolecule material makees catalyst preparation ITO nano wires | |
| CN106950642A (en) | A kind of device for magnetic nano-particle self-assembling photonic crystal optical fiber | |
| CN107270554B (en) | Photo-thermal evaporation device based on plasma resonance and preparation method thereof | |
| CN103900612B (en) | A kind of cold light one solidification equipment and method for optical fibre gyro sensitive optical fibre ring | |
| CN103305884B (en) | Manufacture the method for micro-nano coaxial tube | |
| CN105116534B (en) | Method for capturing and screening particle above topological insulator substrate in tunable manner through linearly-polarized planar optical wave | |
| CN110996412B (en) | Carbon crystal electric heating film and preparation method and application thereof | |
| JP4778616B2 (en) | Optical fiber and unique micropipette with lens with subwavelength aperture | |
| CN106809800B (en) | A kind of preparation method of silicon nanowires/silver-tetracyanoquinodimethane nanowire composite structures |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140129 Termination date: 20180803 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |