CN102346167A - Analysis apparatus - Google Patents
Analysis apparatus Download PDFInfo
- Publication number
- CN102346167A CN102346167A CN2011102193274A CN201110219327A CN102346167A CN 102346167 A CN102346167 A CN 102346167A CN 2011102193274 A CN2011102193274 A CN 2011102193274A CN 201110219327 A CN201110219327 A CN 201110219327A CN 102346167 A CN102346167 A CN 102346167A
- Authority
- CN
- China
- Prior art keywords
- electrode
- analytical equipment
- applying unit
- voltage applying
- mentioned
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44713—Particularly adapted electric power supply
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
Abstract
An analysis apparatus enables a plurality of analysis processes to be accurately and efficiently performed. The analysis apparatus includes a detention tank in which a specimen is stored, and a voltage applier. The voltage applier includes a power source and a contact tip to be brought into contact with the specimen for applying a voltage necessary for analyzing the specimen. The voltage applier renews the contact tip from a used state to an unused state after completing an analysis and before starting the subsequent analysis.
Description
Technical field
The present invention relates to and a kind of analytical equipment that for example uses electrophoresis.
Background technology
Be contained in the concentration or the quantitative analysis method of the special component in the sample as analysis package, for example extensively implement to use the analytical approach of capillary electrophoresis.Capillary electrophoresis is in the less separation stream of sectional area, to fill migration liquid, and then near an end of above-mentioned separation stream, imports said sample.When the two ends of above-mentioned separation stream apply voltage, because of electrophoresis produces the electro-osmosis stream that above-mentioned migration liquid moves to negative side from side of the positive electrode.Through applying above-mentioned voltage, above-mentioned special component moves according to electrophoretic mobility separately.Therefore, above-mentioned special component is according to the velocity of above-mentioned electro-osmosis stream and the synthetic velocity of the velocity that moves of above-mentioned electrophoresis are moved.Move through this, above-mentioned special component is separated from other compositions.For example adopt optical means to detect the special component that this is separated, so just can analyze the amount and the concentration of above-mentioned special component.
Figure 13 representes existing one routine analytical equipment (for example, with reference to JP2009-145245A1).Analytical equipment X shown in this figure comprises microchip 91 and voltage applying unit 92.Be formed with lead-in groove 911, drain tank 913 and be connected their separation stream 912 at microchip 91.Before analysis, lead-in groove 911 with separate stream 912 in be filled with migration liquid.Storage has the sample S as analytic target such as blood for example in sample receiver B.Sample S is directed in the lead-in groove 911 through distributing nozzle 93.Voltage applying unit 92 comprises power supply 921 and two electrodes 922,923.When analyzing, electrode 922 is submerged in the lead-in groove 911, and electrode 923 is submerged in the drain tank 913.When 922,923 at two electrodes apply the voltage of regulation, the special component of beginning separation electrophoresis.Dispose illuminating part 941 and light accepting part 942 to clip the mode of part midway of separating stream 912.Light from light source 943 is fed into illuminating part 941.Light accepting part 942 is connected with test section 944.Utilize test section 944 to measure the for example absorbance of sample S, so just can measure the concentration of the special component of sample S.
But when carrying out certain analysis, sample S can be attached on the electrode 922.When applying voltage and be used for electrophoresis, so, the composition of sample S and above-mentioned migration liquid will be separated out on electrode 922.If use the electrode 922 of this state to carry out next one analysis, so, different sample S just might mix each other, and above-mentioned composition of separating out might be sneaked into and is used for the next sample S that analyzes.For fear of this thing happens, for example, when each the analysis, the user changes electrode 922 through handwork, very inconvenience.
Summary of the invention
The present invention accomplishes according to above-mentioned situation, its purpose be to provide a kind of can be suitably and the analytical equipment of repeatedly analyzing efficiently.
Analytical equipment provided by the invention possesses: the delay tank that is detained liquid; And voltage applying unit, comprising: power supply; With apply aforesaid liquid and analyze the contact of required voltage and apply portion through contacting with aforesaid liquid; Above-mentioned voltage applying unit applies portion with above-mentioned contact and is updated to unused state from using (using) state after certain analysis of end and before beginning next analysis.
Preferred above-mentioned voltage applying unit has a plurality of electrodes, makes these electrodes impregnated in above-mentioned delay tank successively, and the part that impregnated in aforesaid liquid in the above-mentioned electrode is applied portion as above-mentioned contact.
Preferred above-mentioned voltage applying unit also possesses maintaining part, and above-mentioned a plurality of electrodes that this maintaining part will be the configuration of concentric circles ground keep, and rotate freely around above-mentioned concentrically ringed center.
Preferred above-mentioned maintaining part has the conductive part that is used to make above-mentioned power supply and above-mentioned each electrode conduction.
Preferred above-mentioned each electrode is the length direction of bar-shaped and above-mentioned each electrode and the rotating shaft parallel of above-mentioned maintaining part.
Preferred above-mentioned each electrode be bar-shaped and above-mentioned each electrode be configured to length direction along with vertical radially (length direction of above-mentioned each electrode with perpendicular to the turning axle of above-mentioned maintaining part radially consistent) of the turning axle of above-mentioned maintaining part.
Preferably be provided with the through hole that connects in the longitudinal direction at above-mentioned each electrode, above-mentioned voltage applying unit also possesses the distributing nozzle that imports aforesaid liquid through above-mentioned through hole.
Preferred above-mentioned distributing nozzle makes above-mentioned each electrode advance to above-mentioned delay tank.
Preferred above-mentioned voltage applying unit also possesses wiper mechanism, and this wiper mechanism cleans the electrode that above-mentioned contact applies portion that is not used as in above-mentioned a plurality of electrodes.
Preferred above-mentioned wiper mechanism carries out the injection and the discharge of cleaning fluid for the above-mentioned electrode as above-mentioned cleaning object.
Preferred above-mentioned wiper mechanism is to the above-mentioned electrode application voltage as above-mentioned cleaning object.
Preferred above-mentioned a plurality of electrodes are bar-shaped separately; Above-mentioned voltage applying unit also has maintaining part; This maintaining part, keeps above-mentioned a plurality of electrodes under the state of the length direction tandem configuration of above-mentioned a plurality of electrodes at above-mentioned a plurality of electrodes with the mode that can on this length direction, slide.
Preferred above-mentioned maintaining part has the conductive part that makes above-mentioned power supply and above-mentioned each electrode conduction.
Preferably be provided with the through hole that connects in the longitudinal direction at above-mentioned each electrode, above-mentioned voltage applying unit also has the distributing nozzle that imports aforesaid liquid through above-mentioned through hole.
Preferred above-mentioned voltage applying unit has: bar-shaped electrode; With the cutting unit that cuts off this electrode with the mode of cutting apart in the longitudinal direction.
Preferably be formed with at above-mentioned electrode: a plurality of tapered portion that configuration, and the sectional area in the cross section vertical with length direction is in the longitudinal direction partly dwindled with being separated from each other, above-mentioned cutting unit cuts off above-mentioned electrode in above-mentioned tapered portion.
Preferred above-mentioned voltage applying unit possesses: banded electrode; Accommodate the also delivery wheel of the above-mentioned electrode of feeding wrapper state; With the reel of the above-mentioned electrode that transfers out from above-mentioned delivery wheel of reeling, the part that impregnated in aforesaid liquid in the above-mentioned electrode is used as above-mentioned contact and applies portion.
Preferably also have highlight bar, this highlight bar makes the part between above-mentioned delivery wheel and above-mentioned reel in the above-mentioned electrode circuitous to above-mentioned delay tank.
Preferred above-mentioned highlight bar is made up of conductive material, and is connected with above-mentioned power supply.
Electrophoresis is used in preferred above-mentioned analysis.
Preferably import the sample that is used to above-mentioned analysis to above-mentioned delay tank.
According to this structure, when using above-mentioned analytical equipment to analyze, can use the above-mentioned contact of new state to apply portion.Therefore, even cause being attached with sample or separating out the composition that is included in the migration liquid in the above-mentioned contact portion of applying because of carrying out certain analysis, they can not sneak into migration liquid and the sample that is used for next analysis yet.Therefore, can suitably and efficiently analyze a plurality of samples.
With reference to the detailed description that accompanying drawing carried out, other features and advantages of the present invention will be clearer and more definite through following.
Description of drawings
Fig. 1 is the one-piece construction figure of an example of expression analytical equipment of the present invention.
Fig. 2 is the major part sectional view along the II-II line of Fig. 1.
Fig. 3 is the major part sectional view of the state after the expression distributing nozzle advances.
Fig. 4 is the major part sectional view that expression imports the state of sample.
Fig. 5 is the major part sectional view of variation that the voltage applying unit of wiper mechanism is disposed in expression.
Fig. 6 is the major part sectional view that the expression electrode cleans.
Fig. 7 is the major part sectional view of other variation of expression voltage applying unit.
Fig. 8 is the major part sectional view of other variation of expression voltage applying unit.
Fig. 9 is the major part sectional view that the electrode in the variation of presentation graphs 8 is changed.
Figure 10 is the major part sectional view of other variation of expression voltage applying unit.
Figure 11 is illustrated in the major part sectional view that cuts off the state behind the electrode in the variation of Figure 10.
Figure 12 is the major part sectional view of other variation of expression voltage applying unit.
Figure 13 is the one-piece construction figure of the existing routine analytical equipment of expression.
Embodiment
Below, with reference to accompanying drawing, preferred implementation of the present invention is carried out bright specifically.
Fig. 1 representes of the present invention one routine analytical equipment.The analytical equipment A of this embodiment comprises: microchip 1, voltage applying unit 2, analytical equipment 6 and control part 7.In this embodiment, analytical equipment A uses capillary electrophoresis to analyze.
Separate the place that stream 12 is to use capillary electrophoresis to analyze, generally form trickle stream.If enumerate a routine size of separating stream 12, its cross sectional shape preferably diameter is that the circle or the length of side of 25～100 μ m is the rectangle of 25～100 μ m, and length is preferably about 30mm, but is not to be defined in this.
A plurality of electrodes 3 are submerged in the lead-in groove 11, for example are made up of Cu.As shown in Figure 2, be formed with through hole 31 at each electrode 3.Like this, of the back, each electrode 3 is also annotated nozzle performance function as being used for the branch that branch annotates sample S.In this embodiment, each electrode 3 integral body are slightly cylindrical, and its lower portion divides formation towards the diminishing shape of lower end diameter.
Maintaining part 4 is used for keeping a plurality of electrodes 3, has main body 41, turning axle 42 and conductive part 43.Main body 41 for example is made up of resin, forms thick disc-shape.Turning axle 42 is installed in the central authorities of main body 41.In this embodiment, conductive materials such as the turning axle 42 whole Cu of employing constitute, and perhaps its part has current-carrying part, and is connected with the terminal of power supply unit 21.As shown in Figure 1, in the binding of the upper end of turning axle 42 motor 24 is arranged.Motor 24 for example is a servomotor, can make the angle of maintaining part 4 rotation expections.
Be formed with a plurality of retaining holes 411 in main body 41.In this embodiment, the number of retaining hole 411 is 8.A plurality of retaining holes 411 are that the center is the concentric circles configuration with turning axle 24.Each retaining hole 411 connects main body 41 abreast with turning axle 24, and electrode 3 can be kept slidably.Thus, in this embodiment, the posture of 8 electrodes 3 to be parallel to each other is that the center is the concentric circles configuration with turning axle 24.Conductive part 43 for example is made up of Cu, has: the part that contacts with turning axle 24; Part with the inside surface that is exposed to each retaining hole 411.So a plurality of electrodes 3 are through turning axle 24 and the terminal conducting of conductive part 43 with power supply unit 21.
Distributing nozzle 23 is used for importing sample S through electrode 3, and is as shown in Figure 1, has the sample receiver B of sample S to be connected through flexible pipe etc. with storage.In this embodiment, the external diameter of the below part of distributing nozzle 23 is more smaller than the internal diameter of the through hole 31 of electrode 3.When analyzing, will become as the hemodilution of sample S under the situation of normal concentration at needs, be connected with sample air mix facilities not shown in the figures at distributing nozzle 23.Actuator 25 is the drive sources that are used for making distributing nozzle 23 up-downs.
Below, the action when operational analysis device A is analyzed describes.
At first, as shown in Figure 2, the lead-in groove 11 of microchip 1 with separate stream 12 in fill migration liquid Lq as buffering agent.In addition, make any electrode of a plurality of electrodes 3 be positioned at lead-in groove 11 directly over.Below, as shown in Figure 3, according to instruction from above-mentioned control part 7, utilize actuator 25 make distributing nozzle 23 towards be positioned at lead-in groove 11 directly over electrode 3 descend.So the through hole 31 of electrode 3 is partly invaded in the below of distributing nozzle 23.Like this, distributing nozzle 23 is integrally formed with electrode 3.
When utilizing actuator 25 that distributing nozzle 23 is further descended, so as shown in Figure 4, the end portion of electrode 3 is immersed among the migration liquid Lq of lead-in groove 11.The part of this dipping is that contact applies portion 32.Below, utilize the conveying action of pump not shown in the figures, in lead-in groove 11, divide the sample S that annotates ormal weight through distributing nozzle 23 and electrode 3.That is, electrode 3 is also as dividing the branch of annotating sample S to annotate nozzle performance function.Thus, the contact that becomes electrode 3 applies portion 32 and is submerged in the state among the sample S that is stranded in the lead-in groove 11.Under this state,, apply voltage through electrode 3 and discharge lateral electrode 22 from power supply unit 21 according to the instruction of control part 7.Thus, in separating stream 12, produce electrophoresis.Through this electrophoresis, for example, the haemoglobin of Alc etc. moves in separating stream 12.Measure absorbance through operational analysis device 6, haemoglobin (Alc) concentration that can analytical sample S.
After analyzing end,, utilize actuator 25 that distributing nozzle 23 is risen according to the instruction of control part 7.In this rising, electrode 3 is risen.As the mechanism that is used to realize this rising, for example can be set forth in the spring (diagram is omitted) that maintaining part 4 setting applies elastic force, be used for each electrode 3 of jack-up to make it become state shown in Figure 2 from state shown in Figure 4.Perhaps, also can be provided with and be used for making electrode 3 rises other actuator (diagram is omitted).Before implementing next the analysis,, utilize motor 24 to make maintaining part 4 rotation predetermined angulars (being 45 degree) in this embodiment according to the instruction of control part 7.Thus, the electrode 3 that is maintained at electrode 3 next doors that are used for above-mentioned analysis be positioned at lead-in groove 11 directly over.The result is that contact is applied portion 32 is updated to unused state from the state of finishing using EO.Contact is implemented above-mentioned analysis operation after applying the renewal end of portion 32 once more.Through carrying out these analyses and the renewal that contacts the portion of applying 32 repeatedly, embodiment is as with the analysis of a plurality of different sample S as object continuously.
Below, the effect of analytical equipment A is described.
According to this embodiment, when operational analysis device A analyzes, can use the electrode 3 under the new state.Therefore, analyze through carrying out certain, even be attached with sample S or the one-tenth that is included among the migration liquid Lq analyzes at electrode 3, they can not sneaked into yet and be supplied among the next migration liquid Lq and sample S that analyzes.Therefore, can suitably analyze a plurality of sample S.In addition, the replacing of electrode 3 is that instruction according to control part 7 utilizes motor 24 to make maintaining part 4 rotations and carries out automatically.Therefore, manually change the situation of electrode 3 with for example user and compare, can carry out a plurality of analyses at short notice efficiently.
Keep a plurality of electrodes 3 through be concentric circles ground in maintaining part 4, like this,, very convenient as long as rotation maintaining part 4 just can be configured in new electrode 3 on the position that can use.Through being provided with conductive part 43, like this,, just can make each electrode 3 and power supply unit 21 conductings as long as make maintaining part 4 keep a plurality of electrodes 3 in maintaining part 4.
As stated, electrode 3 performances are as dividing the function of annotating nozzle.Thus, when making electrode 3 be positioned at the position under the state that can apply voltage, can carry out the branch of sample S apace and annotate.This helps shortening analysis time.The structure of using distributing nozzle 23 that electrode 3 is descended is suitable for carrying out applying voltage in the lump and dividing the operation of annotating two processing.
Fig. 5～Figure 12 representes the variation of the voltage applying unit 2 of analytical equipment A.In these accompanying drawings, to identical with above-mentioned embodiment or the similar parts element annotation Reference numeral identical with above-mentioned embodiment.
Fig. 5 representes a variation of the voltage applying unit of analytical equipment A.In this variation, voltage applying unit 2 also comprises wiper mechanism 5.Wiper mechanism 5 has rinse bath 51 and injects nozzle 52.Rinse bath 51 for example is configured in respect to the electrode directly over the lead-in groove that is positioned at microchip 1 11 3, be positioned at across turning axle 42 opposition side electrode 3 under.Inject nozzle 52 be configured in be positioned at rinse bath 51 directly over the top of electrode 3, and have the cleaning liquid bath (diagram is omitted) of cleaning fluids such as migration liquid Lq to be connected with for example accumulating.
Fig. 6 representes to use the cleaning action of wiper mechanism 5.As shown in the drawing, when cleaning, for example utilize with actuator 25 same actuators (diagram is omitted) injection nozzle 52 is descended towards electrode 3.Then, make electrode 3 get into rinse bath 51.Then, will inject rinse bath 51 from injecting nozzle 52 as the for example migration liquid Lq of cleaning fluid through electrode 3.In addition, after the migration liquid Lq with ormal weight imports rinse bath 51, through electrode 3 and the migration liquid Lq that injects nozzle 52 discharge rinse baths 51.This injection and discharge for example are to carry out through pump not shown in the figures.
According to this structure,, can clean sample S that adheres at electrode 3 and the composition of separating out through should injecting and discharge repeatedly.Therefore, can the electrode that be used to analyze 3 be used for analyzing once more, and can further improve the efficient of analysis.
As said wiper mechanism among the present invention, except above-mentioned wiper mechanism 5, also can apply the structure that forms by applications exploiting voltage.For example, the composition of migration liquid Lq or sample S is separated out because of the voltage in repeatedly analyzing applies on the surface of electrode 3 sometimes.In this case, under the state in electrode 3 being got into be full of the rinse bath 51 of migration liquid Lq etc., the voltage of antipole property when electrode 3 is applied and analyzes.Like this, can remove the material of separating out through the electricity decomposition at electrode 3.As other method, for example can be applied in the air etc. when electrode 3 applied than analyzed obviously high voltage method.According to this structure, can remove material through decomposing or burning attached to electrode 3.
Fig. 7 representes other variation of voltage applying unit.In this variation, the configuration of a plurality of electrodes 3 is different with above-mentioned voltage applying unit 2.As shown in the drawing, be in maintaining part 4 and form a plurality of retaining holes 411 radially.Through being kept by these retaining holes, a plurality of electrodes 3 are configuration radially.In this variation,, also can new electrode 3 be configured in the use location, and can carry out suitable analysis efficiently through making maintaining part 4 rotation predetermined angulars.
In this variation, wiper mechanism 5 is made up of washer jet 53.Washer jet 53 is according to the instruction of control part 7, and any electrode 3 beyond the electrode that is positioned at the use location 3 sprays cleaning fluids such as migration liquid Lq.Use this wiper mechanism 5 also can the electrode that use 3 be cleaned and become the state that can use.
Fig. 8 representes other variation of voltage applying unit 2.The structure of the maintaining part 4 of the voltage applying unit 2 of this variation is different with above-mentioned voltage applying unit with the hold mode of a plurality of electrodes 3.As shown in the drawing, maintaining part 4 forms long drum.Main body 41 is drums, and side is provided with conductive part 43 within it.A plurality of electrodes 3 are held portion 4 and keep under the state that the posture tandem consistent with the length direction of maintaining part 4 according to its length direction arranged.In addition, a plurality of electrodes 3 are held portion's 4 maintenances with the mode that can slide.In this variation, the part in the retaining hole 411 of the entering maintaining part 4 in the distributing nozzle 23 is the length with maintaining part 4 same degree.In addition, utilize above-mentioned actuator 25 that distributing nozzle 23 is freely descended with interim ground, each predetermined distance ground.
In the analysis of the voltage applying unit 2 that uses this variation, the end portion that is arranged in the electrode 3 of lower side applies the migration liquid Lq that portion 32 is submerged in lead-in groove 11 as contact.After analyzing end, as shown in Figure 9, make maintaining part 4 from keeping out of the way position directly over the lead-in groove 11 to other.Then, make distributing nozzle 23 decline predetermined distances.Like this, the electrode 3 that is positioned at the lower side is from maintaining part 4 disengagings.Then, the new electrode that is positioned at the lower side 3 is used for next the analysis.Also can be suitably and carry out a plurality of analyses efficiently according to this variation.
Figure 10 representes other variation of voltage applying unit 2.The voltage applying unit 2 of this variation mainly is that the structure of its electrode 3 is different with above-mentioned voltage applying unit.Voltage applying unit 2 shown in this figure comprises an electrode 3.This electrode 3 is longer, has a plurality of cut-out portion 33.A plurality of cut-out portion 33 separates separate configuration equally spacedly on the length direction of electrode 3.The part of each cut-out portion 33 attenuates (centre attenuates), compares with its peripheral part, and its sectional area diminishes.In addition, maintaining part 4 integral body constitute conductive part 43, but are used for keeping electrode 3 and free lifting.In this variation, voltage applying unit 2 disposes a pair of cutoff tool 34.A pair of cutoff tool 34 is equivalent to an example of said cutting unit among the present invention, and the cut-out portion that is positioned at the lower side in a plurality of cut-out portion 33 can cut off (cutting) electrode 3.Apply portion 32 through the contact that makes electrode 3 and get into and carry out in the lead-in grooves 11 that branch is annotated and voltage applies, this point is identical with above-mentioned voltage applying unit 2.
After finishing certain and analyzing, shown in figure 11, make maintaining part 4 and electrode 3 move to other position directly over the lead-in groove 11.Then, utilize a pair of cutoff tool 34 to cut off electrode 3 in cut-out portion 33.Like this, can be with being attached with sample S because of analysis or moving the part that the one-tenth of liquid Lq analyzes and separate from electrode 3.Then, the part that becomes new lower end in the electrode 3 is used as contact and applies portion 32 in next one analysis.According to this variation, also can be suitably and carry out a plurality of analyses efficiently.
Figure 12 representes other variation of voltage applying unit 2.Voltage applying unit shown in this figure comprises: delivery wheel 35, reel 36, highlight bar 37 and pair of rolls 38.In this variation, electrode 3 is long ribbon shapes, and various piece can be crooked.Delivery wheel 35 is taken in the electrode 3 of reeling condition, and can delivery electrodes 3.Reel 36 is used for the electrode 3 of reeling and transporting from delivery wheel 35, for example disposes motor not shown in the figures.This motor is according to the instruction of control part 7 realize the reeling operation of electrode 3 of ormal weight.
After finishing certain and analyzing,, be utilized in the above-mentioned motor that disposed in the reel 36 with the reel length of regulation of electrode 3 according to the instruction of control part 7.Like this, the part on the top of the covering highlight bar 37 in the electrode 3 is a side shifting downstream, and the top of highlight bar 37 is covered by other parts of electrode 3.The result is that contact applies portion 32 and is updated to untapped state from the state that uses.According to this variation, also can be suitably and carry out a plurality of analyses efficiently.In addition,, need not discarded certain electrode 3 or cut off electrode 3, just can successfully contact the renewal of the portion of applying 32 according to this variation.
Analytical equipment of the present invention is not to be defined in above-mentioned embodiment.The concrete structure of the various piece of analytical equipment of the present invention can freely carry out various design alterations.
Claims (21)
1. analytical equipment is characterized in that possessing:
The delay tank that is detained liquid; With
Voltage applying unit comprises: power supply; With apply portion through contact the contact that applies the required voltage of said fluid analysis with said liquid,
Said voltage applying unit applies portion with said contact and is updated to unused state from the state of using after certain analysis of end and before beginning next analysis.
2. analytical equipment as claimed in claim 1 is characterized in that:
Said voltage applying unit has a plurality of electrodes,
Make these electrodes impregnated in said delay tank successively, the part that impregnated in said liquid in the said electrode is applied portion as said contact.
3. analytical equipment as claimed in claim 2 is characterized in that:
Said voltage applying unit also possesses maintaining part, and said a plurality of electrodes that this maintaining part will be the configuration of concentric circles ground keep, and rotate freely around said concentrically ringed center.
4. analytical equipment as claimed in claim 3 is characterized in that:
Said maintaining part has the conductive part that is used to make said power supply and said each electrode conduction.
5. analytical equipment as claimed in claim 3 is characterized in that:
Said each electrode is the length direction of bar-shaped and said each electrode and the rotating shaft parallel of said maintaining part.
6. analytical equipment as claimed in claim 3 is characterized in that:
Said each electrode is bar-shaped and said each electrode and is configured to length direction along vertical radially with the turning axle of said maintaining part.
7. analytical equipment as claimed in claim 5 is characterized in that:
Be provided with the through hole that connects in the longitudinal direction at said each electrode,
Said voltage applying unit also possesses the distributing nozzle that imports said liquid through said through hole.
8. analytical equipment as claimed in claim 7 is characterized in that:
Said distributing nozzle makes said each electrode advance to said delay tank.
9. analytical equipment as claimed in claim 7 is characterized in that:
Said voltage applying unit also possesses wiper mechanism, and this wiper mechanism cleans the electrode that said contact applies portion that is not used as in said a plurality of electrodes.
10. analytical equipment as claimed in claim 9 is characterized in that:
Said wiper mechanism carries out the injection and the discharge of cleaning fluid for the said electrode as said cleaning object.
11. analytical equipment as claimed in claim 9 is characterized in that:
Said wiper mechanism is to the said electrode application voltage as said cleaning object.
12. analytical equipment as claimed in claim 2 is characterized in that:
Said a plurality of electrode is bar-shaped separately,
Said voltage applying unit also has maintaining part, and this maintaining part, keeps said a plurality of electrodes under the state of the length direction tandem configuration of said a plurality of electrodes at said a plurality of electrodes with the mode that can on this length direction, slide.
13. analytical equipment as claimed in claim 12 is characterized in that:
Said maintaining part has the conductive part that makes said power supply and said each electrode conduction.
14. analytical equipment as claimed in claim 13 is characterized in that:
Be provided with the through hole that connects in the longitudinal direction at said each electrode,
Said voltage applying unit also has the distributing nozzle that imports said liquid through said through hole.
15. analytical equipment as claimed in claim 1 is characterized in that:
Said voltage applying unit has: bar-shaped electrode; With the cutting unit that cuts off this electrode with the mode of cutting apart in the longitudinal direction.
16. analytical equipment as claimed in claim 15 is characterized in that:
Be formed with at said electrode: a plurality of tapered portion that configuration, and the sectional area in the cross section vertical with length direction is in the longitudinal direction partly dwindled with being separated from each other,
Said cutting unit cuts off said electrode in said tapered portion.
17. analytical equipment as claimed in claim 1 is characterized in that:
Said voltage applying unit possesses: banded electrode; Accommodate the also delivery wheel of the said electrode of feeding wrapper state; With the reel of the said electrode that transfers out from said delivery wheel of reeling,
The part that impregnated in said liquid in the said electrode is used as said contact and applies portion.
18. analytical equipment as claimed in claim 17 is characterized in that:
Also have highlight bar, this highlight bar makes the part between said delivery wheel and said reel in the said electrode circuitous to said delay tank.
19. analytical equipment as claimed in claim 18 is characterized in that:
Said highlight bar is made up of conductive material, and is connected with said power supply.
20. analytical equipment as claimed in claim 1 is characterized in that:
Electrophoresis is used in said analysis.
21. analytical equipment as claimed in claim 20 is characterized in that:
Import the sample that is used to said analysis to said delay tank.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010-174133 | 2010-08-03 | ||
| JP2010174133A JP5462738B2 (en) | 2010-08-03 | 2010-08-03 | Analysis equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102346167A true CN102346167A (en) | 2012-02-08 |
| CN102346167B CN102346167B (en) | 2014-03-05 |
Family
ID=44674204
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110219327.4A Active CN102346167B (en) | 2010-08-03 | 2011-08-02 | Analysis apparatus |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US8506785B2 (en) |
| EP (1) | EP2416148B1 (en) |
| JP (1) | JP5462738B2 (en) |
| CN (1) | CN102346167B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103245704A (en) * | 2013-05-02 | 2013-08-14 | 中国科学院合肥物质科学研究院 | Automatic electrode replacing device of online heavy metal analyzer |
| CN113366607A (en) * | 2019-02-19 | 2021-09-07 | 株式会社岛津制作所 | Mass spectrometer |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101424758B1 (en) * | 2012-12-27 | 2014-08-01 | 한국광해관리공단 | Portable heavy metal detector of ground water with mine drainage and method for measuring pollution level of groudwater with mine drainage thereof |
| EP3093656A1 (en) * | 2015-05-13 | 2016-11-16 | ARKRAY, Inc. | Analytical tool and analytical system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6207031B1 (en) * | 1997-09-15 | 2001-03-27 | Whitehead Institute For Biomedical Research | Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device |
| US20010005489A1 (en) * | 1998-07-02 | 2001-06-28 | Roach David J. | Apparatus and method for filling and cleaning channels and inlet ports in microchips used for biological analysis |
| WO2008078781A1 (en) * | 2006-12-26 | 2008-07-03 | Sekisui Chemical Co., Ltd. | Hemoglobin measurement method and electrophoresis apparatus |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0635069B2 (en) * | 1986-02-25 | 1994-05-11 | 小池酸素工業株式会社 | Plasma cutting torch tip changer |
| JPS62250464A (en) * | 1986-04-23 | 1987-10-31 | Matsushita Electric Ind Co Ltd | Electrostatic charger |
| JPS632573A (en) * | 1986-06-23 | 1988-01-07 | Toshiba Corp | Electrode changing device |
| JPH0533158U (en) * | 1991-10-07 | 1993-04-30 | 株式会社リコー | Corona charging device |
| JP2662682B2 (en) * | 1992-01-23 | 1997-10-15 | 三菱電機株式会社 | Wire electrode automatic feeder |
| JP2566910Y2 (en) * | 1992-03-18 | 1998-03-30 | 株式会社アマダ | Torch changer for plasma processing machine |
| JPH07104314B2 (en) * | 1992-09-09 | 1995-11-13 | 工業技術院長 | Carbon sensor electrode and method of manufacturing the same |
| JP3420311B2 (en) * | 1993-12-24 | 2003-06-23 | 理化学研究所 | Electrophoresis device |
| JP2002131282A (en) * | 2000-10-24 | 2002-05-09 | Hitachi Ltd | Capillary array |
| US6878256B2 (en) * | 2001-04-02 | 2005-04-12 | Hitachi, Ltd. | Capillary array device |
| JP2007506092A (en) * | 2003-09-22 | 2007-03-15 | 株式会社島津製作所 | Electrophoresis apparatus and method, and electrophoresis member and sample dispensing probe |
| JP4422658B2 (en) * | 2005-08-10 | 2010-02-24 | 株式会社日立ハイテクノロジーズ | Liquid dispensing device |
| JP4441653B2 (en) * | 2005-08-31 | 2010-03-31 | シャープ株式会社 | Automated two-dimensional electrophoresis apparatus and apparatus components |
| JP2009145245A (en) | 2007-12-14 | 2009-07-02 | Sekisui Chem Co Ltd | Hemoglobin measurement method |
| DE102007061806A1 (en) * | 2007-12-19 | 2009-06-25 | Mettler-Toledo Ag | Process for the regeneration of amperometric sensors |
-
2010
- 2010-08-03 JP JP2010174133A patent/JP5462738B2/en active Active
-
2011
- 2011-08-02 CN CN201110219327.4A patent/CN102346167B/en active Active
- 2011-08-02 US US13/196,578 patent/US8506785B2/en active Active
- 2011-08-03 EP EP11176429.6A patent/EP2416148B1/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6207031B1 (en) * | 1997-09-15 | 2001-03-27 | Whitehead Institute For Biomedical Research | Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device |
| US20010005489A1 (en) * | 1998-07-02 | 2001-06-28 | Roach David J. | Apparatus and method for filling and cleaning channels and inlet ports in microchips used for biological analysis |
| WO2008078781A1 (en) * | 2006-12-26 | 2008-07-03 | Sekisui Chemical Co., Ltd. | Hemoglobin measurement method and electrophoresis apparatus |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103245704A (en) * | 2013-05-02 | 2013-08-14 | 中国科学院合肥物质科学研究院 | Automatic electrode replacing device of online heavy metal analyzer |
| CN113366607A (en) * | 2019-02-19 | 2021-09-07 | 株式会社岛津制作所 | Mass spectrometer |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2416148B1 (en) | 2016-05-11 |
| US8506785B2 (en) | 2013-08-13 |
| JP5462738B2 (en) | 2014-04-02 |
| CN102346167B (en) | 2014-03-05 |
| US20120031761A1 (en) | 2012-02-09 |
| EP2416148A3 (en) | 2013-09-04 |
| EP2416148A2 (en) | 2012-02-08 |
| JP2012032350A (en) | 2012-02-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102346167A (en) | Analysis apparatus | |
| Zhu et al. | On-line concentration of acidic compounds by anion-selective exhaustive injection-sweeping-micellar electrokinetic chromatography | |
| NO20056072L (en) | Sample set up / assembly device method and apparatus utilizing the sample assembly | |
| ATE401245T1 (en) | DOSING DEVICE FOR RADIOACTIVE FLUID | |
| Fotouhi et al. | Evaluation of pulsed electromembrane extraction for the analysis of diclofenac and mefenamic acid in biological fluids | |
| Kubáň et al. | Preconcentration in micro-electromembrane extraction across free liquid membranes | |
| WO2021099610A1 (en) | Wearable systems for measuring sweat rate and methods of using the same | |
| Wei et al. | On-line concentration of proteins and peptides in capillary zone electrophoresis with an etched porous joint | |
| DE69832488D1 (en) | Sample injection device for a high performance liquid chromatograph | |
| US10866212B2 (en) | Analytical tool and analytical system | |
| RU2002129927A (en) | METHOD AND DEVICE FOR MICRODOSING MINIMUM QUANTITIES OF LIQUID FOR BIOPOLYMERIC MATRICES | |
| GB2264783A (en) | Electrophoretic analysis method utilising wave effect | |
| KR20030003718A (en) | Method and Device for Microdosing the Smallest Amounts of Liquid for Biopolymer Arrays | |
| WO1994026396A1 (en) | Application specific capillary electrophoresis | |
| US2868020A (en) | Apparatus for applying a liquid sample | |
| US3495446A (en) | Applicator for chromatography and electrophoresis | |
| KR101855608B1 (en) | Biomolecular analysis apparatus | |
| US9645108B2 (en) | Scanning drop sensor | |
| CN105572207A (en) | Convenient capillary three-dimensional sample injection device and use method | |
| Liu et al. | A falling drop for sample injection in capillary zone electrophoresis | |
| CN104215680B (en) | Capillary Electrophoresis speed difference pattern measures the device of compound Enantiomer excess value | |
| Wu et al. | In-capillary preconcentration of proteins for capillary electrophoresis using a cellulose acetate-coated porous joint | |
| CZ257898A3 (en) | Process and apparatus for feeding a sample into a capillary electrophoretic apparatus | |
| FR2069452A5 (en) | Continuous electrophoresis of a series of - samples | |
| EP2913664A1 (en) | Chip for plasma generation, plasma generator, and plasma spectrometry method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |