CN100520394C - Single-channel multi-parameter surface plasms resonance tester - Google Patents
Single-channel multi-parameter surface plasms resonance tester Download PDFInfo
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- CN100520394C CN100520394C CNB2005100535106A CN200510053510A CN100520394C CN 100520394 C CN100520394 C CN 100520394C CN B2005100535106 A CNB2005100535106 A CN B2005100535106A CN 200510053510 A CN200510053510 A CN 200510053510A CN 100520394 C CN100520394 C CN 100520394C
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- surface plasma
- plasma resonance
- chip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
Abstract
A resonant ester of single channel and multiple parameter surface plasma consists of control unit, optical unit, mechanical scanning unit, circulation unit, surface plasma resonant chip, signal collection and process software, operation and control software. It is featured as realizing angular modulation by mechanical scanning unit for carrying out signal detection of angle and light intensity, applying two units or multiple units of surface plasma resonant chips to provide corresponding detection results of double parameter or multiple parameter when sample to be tested flows through chip via single channel.
Description
Technical field
The invention belongs to testing tool technical field, particularly single-channel multi-parameter surface plasms resonance (SPR) tester.
Background technology
At present, single channel surface plasma resonance (SPR) tester is only measured a parameter at every turn, and each passage of multiple channel surface plasma body resonance (SPR) tester is also only measured a parameter, causes the test process complexity, data volume is big, and is consuming time and make mistakes easily.
Summary of the invention
The objective of the invention is to propose a kind of novel single-channel multi-parameter surface plasms resonance (SPR) tester, this instrument adopts two unit chips, cooperates corresponding optical system, detection system and information handling system to realize two-parameter detection; Or adopt multiple-unit chip and corresponding signal acquiring processing system, also can realize the single channel multi-parameters test.
For achieving the above object, technical solution of the present invention provides a kind of single-channel multi-parameter surface plasms resonance tester, comprise parts such as control system, optical system, mechanical scanning system, the system for the distribution of commodities and surface plasma resonance chip, signal acquisition process software and system operation Control Software, constitute complete surface plasma resonance test macro, wherein, realize angular modulation with the mechanical motion Scan Architecture, carry out angle and light intensity signal single channel and detect; It adopts multiple-unit surface plasma resonance chip, when testing sample flows through chip through single channel, can provide corresponding multiparameter testing result.
Described surface plasma resonance test instrument, its described system for the distribution of commodities comprises test pool, pipeline, feed liquor valve, peristaltic pump and sensitive chip; Wherein, the optical prism below closely is connected with the golden film of surface plasma resonance multiple-unit sensitive chip, and golden film below closely is connected with test pool; The test pool two ends are provided with feed tube and drain pipe, and feed tube is connected with the feed liquor valve, and the feed liquor valve is provided with two imports, and an import is communicated with sample hose, and another import is communicated with the carrier fluid pipe, on the carrier fluid pipe peristaltic pump is housed; Sample contacts with system for the distribution of commodities surface plasma resonance multiple-unit sensitive chip in the measuring cell, provides corresponding multiparameter testing result.
Described surface plasma resonance test instrument, its described surface plasma resonance chip is the naked gold surface plasma resonance chip of block form; Multiple-unit surface plasma resonance chip is the naked gold surface plasma resonance chip of two unit block forms at least.
Described surface plasma resonance test instrument, its described mechanical scanning system, comprise optical table, connection rod set, slide block, guide rail, screw rod, motor, angular displacement sensor and pull bar, wherein, connection rod set is made up of four connecting rods, and four connecting rods surround rhombus, four connecting rods can be that rotate in the axle center to connect point, one connects some B and is installed on the optical table, and another relative company's point A is connected with pull bar, and the pull bar other end connects slide block; One guide rail is arranged under slide block, and slide block can move on guide rail; The slide block other end is connected to screw rod, and the screw rod other end and motor transmission shaft are dynamically connected, and screw rod other end end is connected with angular displacement sensor;
Light source and array photodiode are placed on respectively on the connecting rod of company's point B both sides of connection rod set, are positioned in the incident and reflected light path of optical prism; Optical prism, surface plasma resonance chip and measuring cell are combined on the optical table together.
Described surface plasma resonance test instrument, its described mechanical scanning system can carry out angle measurement or the light intensity test under special angle.
Described surface plasma resonance test instrument, its described light source is a semiconductor laser; The array photodiode is at least two unit photodiode array or CCD.
Described surface plasma resonance test instrument, its described light source forms the light beam of certain area by the parallel light path system; During double parameter test, the optical receiver of employing is two unit light electric diodes, and during multi-parameters test, the optical receiver of employing is multiple-unit photodiode array or CCD.
Described surface plasma resonance test instrument, its each measuring multiple parameters result who provides that measures, one of them parameter is a reference value, with the sample measurement value difference of comparing, can eliminate temperature and The noise, improves testing reliability and accuracy.
Described surface plasma resonance test instrument, its control system to regulate beam incident angle accurately, are realized angle scanning according to the travelling speed and the direction of running status decision mechanical scanning system; Acquired signal is divided into two kinds of angle and light intensity, and when mechanical movement during to extreme position, control system is proofreaied and correct angle, and according to operation result mechanical speed is adjusted automatically; At each distinguishable incident angle, system all detects light intensity, and the sampling rate and the sample number of detection are controlled, the reference frame the when result of each run measures as decision is next.
Principal feature of the present invention is in inventor's granted patent: on the basis of the ZL patent No. 98102366.5, except that keeping original surface plasma resonance test instrument basic structure, further expand and sophisticated systems.Usually adopt pointolite to get final product, the present invention has adopted the parallel beam that certain area is arranged, with topped Unit two or multiple-unit chip, so system of the present invention adopts parallel beam light source and Unit two or the multiple-unit photodiode corresponding with chip and the injected system that flows accordingly, and disposes Unit two or collection of multiple-unit chip information and disposal system.Tester of the present invention is simple in structure, and is easy and simple to handle, can do the multiparameter automatic test simultaneously, and test data is reliable, accurate.
Description of drawings
Fig. 1 is the system architecture diagram of single-channel multi-parameter surface plasms resonance of the present invention (SPR) tester;
Fig. 2 is the optics and the mechanical scanning system structural representation of single-channel multi-parameter surface plasms resonance of the present invention (SPR) tester;
Fig. 3 is the system light path and the system for the distribution of commodities structural representation of single-channel multi-parameter surface plasms resonance of the present invention (SPR) tester;
Fig. 4 is the application example test curve figure of the two-parameter surface plasma resonance of single channel of the present invention (SPR) tester.
Embodiment
It is distinctive that apparatus of the present invention not only have surface plasma resonance (SPR) biochemical analysis technology: sample need not mark and does not need purification process, can detect muddy or opaque sample, can Real-time and Dynamic Detection, ranges of incidence angles is wide, angle changes the resolution height, advantages such as adaptability is strong, and also has the detection sensitivity height with respect to one-parameter surface plasma resonance (SPR) tester, need sample size still less, process is convenient and swift, accurately and reliably, applied range and can carry out advantage such as high throughput testing, and, this device has supporting mobile injected system, can realize the two-parameter or multiparameter robotization detection of single channel, in life science, chemical analysis, clinical diagnosis, drug screening, food security, but all widespread uses in environmental monitoring and the relevant scientific research field.
Fig. 1 is a system architecture diagram of the present invention, the composition of tester comprises: four parts such as optical system, mechanical scanning system, the system for the distribution of commodities and data acquisition system (DAS), four parts connect by the standard normal mode, and link with instrument control system and computing machine, constitute complete light, mechanical, electrical integral system.
Fig. 2 is a mechanical scanning system structural principle synoptic diagram.Among the figure, tester pedestal 1, optical table 2, connection rod set 3, slide block 4, guide rail 5, screw rod 6, motor 7, angular displacement sensor 8, pull bar 9, light source 10, two (or many) cell array photodiode 11, optical prism 12 and measuring cell 13.
It is characterized in that, adopt the mechanical scanning structure according to surface plasma resonance (SPR) angle modulation principle.Here said mechanical scanning motion structure comprises optical table 2, connection rod set 3, slide block 4, guide rail 5, screw rod 6, motor 7, angular displacement sensor 8 and pull bar 9, wherein, connection rod set 3 is made up of four connecting rods, four connecting rods surround rhombus, four connecting rods can even be put to rotating in the axle center, one connects some B and is installed on the optical table 2, and another relative company's point A is connected with pull bar 9, and pull bar 9 other ends connect slide block 4.At slide block one guide rail 5 is arranged for 4 times, slide block 4 can move on guide rail 5.Slide block 4 other ends are connected to screw rod 6, and screw rod 6 other ends and motor 7 are dynamically connected, and screw rod 6 other end ends and angular displacement sensor 8 are affixed.
Fig. 3 is system light path, two (or many) cell surface plasma resonance (SPR) chip and system for the distribution of commodities structural representation.Wherein, optical prism 12 belows closely are connected with the golden film 14 of surface plasma resonance two unit sensitive chips 21 or multiple-unit sensitive chip 22, and golden film 14 belows closely are connected with test pool 13.Test pool 13 two ends are provided with feed tube 15 and drain pipe 16, and feed tube 15 is connected with feed liquor valve 17, and feed liquor valve 17 is provided with two imports, and one is communicated with sample hose 18, and another is communicated with carrier fluid pipe 19, and peristaltic pump 20 is housed on the carrier fluid pipe 19.Sample contacts with system for the distribution of commodities surface plasma resonance (SPR) two unit sensitive chips 21 or multiple-unit sensitive chip 22 in the measuring cell 13.
Below in conjunction with accompanying drawing narration the present invention.
As shown in Figure 1, control system to regulate beam incident angle accurately, realizes angle scanning according to the travelling speed and the direction of running status decision mechanical scanning motion structure; Acquired signal is divided into two kinds of angle and light intensity, and when mechanical movement during to extreme position, control system is proofreaied and correct angle, and according to operation result mechanical speed is adjusted automatically.At each distinguishable incident angle, system all detects light intensity, and the sampling rate and the sample number of detection are controlled.Reference frame when the result of each run measures as decision is next.
Fig. 2 as can be known, leverage can accurately be realized micro angular displacement, and simple in structure.The advantage of this structure is to realize that small incident angle changes and variation range greatly, and simultaneously reflected light is followed the tracks of; The sensitivity of system is decided by motor 7 and angular displacement sensor 8 minimum distinguishable angular displacements; The degree of accuracy of system is mainly determined by the manufacturing accuracy of screw rod 6, and the size of the brachium of the length of stroke of slide block 4 and connecting rod decision measurement range; Use when of the present invention, determinand is to be driven and valve control by peristaltic pump, and by being placed on the measuring cell 13 and system for the distribution of commodities surface plasma resonance (SPR) Unit two or multiple-unit sensitive chip contact reaction on the optical table 2, realization is to the detection of determinand.
Figure 4 shows that actual measurement sample curve map when the two-parameter surface plasma resonance of single channel (SPR) tester is used.Among the figure in two curves one (green line) be that reference curve, one are sample curve (red line), with reference value and the sample measurement value difference of comparing, the interference and the influence of extraneous factors such as temperature and noise be can eliminate, testing reliability and accuracy further improved.
Claims (5)
1, a kind of single-channel multi-parameter surface plasms resonance tester, comprise control system, optical system, mechanical scanning system, the system for the distribution of commodities and surface plasma resonance chip, signal acquisition process software and system operation Control Software part, constitute complete surface plasma resonance test macro, wherein, realize angular modulation with the mechanical motion Scan Architecture, carry out angle and light intensity signal single channel and detect; It is characterized in that adopt multiple-unit surface plasma resonance chip, the optical prism below closely is connected with the golden film of multiple-unit surface plasma resonance chip, golden film below closely connects test pool; Described multiple-unit surface plasma resonance chip is the naked gold surface plasma resonance chip of two unit block forms at least; Adopting parallel beam is the naked gold surface plasma resonance chip of two unit block forms to cover at least; Light source is a semiconductor laser; Optical receiving set is at least two cellular array photodiode or CCD; When testing sample flows through chip through single channel, can provide corresponding multiparameter testing result; Each measuring multiple parameters result who provides that measures, one of them parameter is a reference value, with the sample measurement value difference of comparing, can eliminate temperature and The noise, improves testing reliability and accuracy.
2, surface plasma resonance test instrument as claimed in claim 1 is characterized in that, the described system for the distribution of commodities comprises test pool, pipeline, feed liquor valve, peristaltic pump and sensitive chip; Wherein, the test pool two ends are provided with feed tube and drain pipe, and feed tube is connected with the feed liquor valve, and the feed liquor valve is provided with two imports, and an import is communicated with sample hose, and another import is communicated with the carrier fluid pipe, on the carrier fluid pipe peristaltic pump is housed; Sample contacts with system for the distribution of commodities surface plasma resonance multiple-unit sensitive chip in the test pool, provides corresponding multiparameter testing result.
3. surface plasma resonance test instrument as claimed in claim 1, it is characterized in that, described mechanical scanning system comprises optical table, connection rod set, slide block, guide rail, screw rod, motor, angular displacement sensor and pull bar, wherein, connection rod set is made up of four connecting rods, four connecting rods surround rhombus, and four connecting rods can be that rotate in the axle center to connect point, and one connects some B and is installed on the optical table, another relative company's point A is connected with pull bar, and the pull bar other end connects slide block; One guide rail is arranged under slide block, and slide block can move on guide rail; The slide block other end is connected to screw rod, and the screw rod other end and motor transmission shaft are dynamically connected, and screw rod other end end is connected with angular displacement sensor;
Light source and array photodiode are placed on respectively on the connecting rod of company's point B both sides of connection rod set, are positioned in the incident and reflected light path of optical prism; Optical prism, surface plasma resonance chip and test pool are combined on the optical table together.
4. surface plasma resonance test instrument as claimed in claim 3 is characterized in that, described mechanical scanning system can carry out angle measurement or the light intensity test under special angle.
5. as claim 1,2, the described surface plasma resonance test instrument of 3 or 4 arbitrary claims, it is characterized in that, control system to regulate beam incident angle accurately, realizes angle scanning according to the travelling speed and the direction of running status decision mechanical scanning system; Acquired signal is divided into two kinds of angle and light intensity, and when mechanical movement during to extreme position, control system is proofreaied and correct angle, and according to operation result mechanical speed is adjusted automatically; At each distinguishable incident angle, system all detects light intensity, and the sampling rate and the sample number of detection are controlled, the reference frame the when result of each run measures as decision is next.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100535106A CN100520394C (en) | 2005-03-08 | 2005-03-08 | Single-channel multi-parameter surface plasms resonance tester |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100535106A CN100520394C (en) | 2005-03-08 | 2005-03-08 | Single-channel multi-parameter surface plasms resonance tester |
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| CN1831527A CN1831527A (en) | 2006-09-13 |
| CN100520394C true CN100520394C (en) | 2009-07-29 |
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| CNB2005100535106A Expired - Fee Related CN100520394C (en) | 2005-03-08 | 2005-03-08 | Single-channel multi-parameter surface plasms resonance tester |
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| CN102297852A (en) * | 2011-05-24 | 2011-12-28 | 北京理工大学 | Single-shaft driven four-bar mechanism portable SPR detector |
Citations (8)
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|---|---|---|---|---|
| CN1238448A (en) * | 1998-06-08 | 1999-12-15 | 中国科学院电子学研究所 | Surface plasma resonance tester |
| CN1344366A (en) * | 1999-02-01 | 2002-04-10 | 维尔有限公司 | Surface plasmon resonance sensor |
| US6373577B1 (en) * | 1998-05-20 | 2002-04-16 | Graffinity Pharmaceutical Design Gmbh | Surface plasmon resonance sensor for the simultaneous measurement of a plurality of samples in fluid form |
| US6441906B2 (en) * | 1998-11-20 | 2002-08-27 | Graffinity Pharmaceutical Design Gmbh | Set-up of measuring instruments for the parallel readout of SPR sensors |
| US6480282B1 (en) * | 1999-05-06 | 2002-11-12 | University Of Washington | Capillary surface plasmon resonance sensors and multisensors |
| CN1421699A (en) * | 2001-11-23 | 2003-06-04 | 上海数康生物科技有限公司 | Surface plasma resonance biosensor for detecting several biological signals parallelly |
| US20030128364A1 (en) * | 2000-02-22 | 2003-07-10 | Stefan Dickopf | SPR sensor and SPR sensor array |
| JP2004150829A (en) * | 2002-10-28 | 2004-05-27 | Toyobo Co Ltd | Flow cell for surface plasmon resonance measurement |
-
2005
- 2005-03-08 CN CNB2005100535106A patent/CN100520394C/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6373577B1 (en) * | 1998-05-20 | 2002-04-16 | Graffinity Pharmaceutical Design Gmbh | Surface plasmon resonance sensor for the simultaneous measurement of a plurality of samples in fluid form |
| CN1238448A (en) * | 1998-06-08 | 1999-12-15 | 中国科学院电子学研究所 | Surface plasma resonance tester |
| US6441906B2 (en) * | 1998-11-20 | 2002-08-27 | Graffinity Pharmaceutical Design Gmbh | Set-up of measuring instruments for the parallel readout of SPR sensors |
| CN1344366A (en) * | 1999-02-01 | 2002-04-10 | 维尔有限公司 | Surface plasmon resonance sensor |
| US6480282B1 (en) * | 1999-05-06 | 2002-11-12 | University Of Washington | Capillary surface plasmon resonance sensors and multisensors |
| US20030128364A1 (en) * | 2000-02-22 | 2003-07-10 | Stefan Dickopf | SPR sensor and SPR sensor array |
| CN1421699A (en) * | 2001-11-23 | 2003-06-04 | 上海数康生物科技有限公司 | Surface plasma resonance biosensor for detecting several biological signals parallelly |
| JP2004150829A (en) * | 2002-10-28 | 2004-05-27 | Toyobo Co Ltd | Flow cell for surface plasmon resonance measurement |
Non-Patent Citations (2)
| Title |
|---|
| 一种新型表面等离子体谐振（SPR）生化分析系统的研究. 蔡浩原.博士学位论文. 2003 * |
| 表面等离子体谐振（SPR）生化分析仪的研制. 崔大付等.现代科学仪器，第6期. 2001 * |
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| CN1831527A (en) | 2006-09-13 |
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Granted publication date: 20090729 Termination date: 20170308 |