CA2568824A1 - Glucose biosensor and method - Google Patents
Glucose biosensor and method Download PDFInfo
- Publication number
- CA2568824A1 CA2568824A1 CA002568824A CA2568824A CA2568824A1 CA 2568824 A1 CA2568824 A1 CA 2568824A1 CA 002568824 A CA002568824 A CA 002568824A CA 2568824 A CA2568824 A CA 2568824A CA 2568824 A1 CA2568824 A1 CA 2568824A1
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- CA
- Canada
- Prior art keywords
- glucose
- electrode
- measurement
- selecting
- difference
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
- C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/004—Enzyme electrodes mediator-assisted
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
Abstract
A system for more accurately measuring glucose in a sample includes a first glucose-sensing electrode incorporating a quantity of glucose oxidase, a second glucose-sensing electrode incorporating a quantity of PQQ-glucose dehydrogenase, a reference electrode, and means for selecting between a first glucose measurement made with the first glucose-sensing electrode and a second glucose measurement made with the second glucose-sensing electrode.
Claims (16)
1. A glucose biosensor comprising:
a laminated body having a fluid sample inlet end and an electrical contact end;
a fluid sample inlet;
a substantially flat sample chamber in communication between said fluid sample inlet and a vent opening, said test chamber being adapted to collect a fluid sample through said fluid sample inlet;
a GOD-based glucose electrode;
a GDH-based glucose electrode; and a reference electrode wherein said GOD-based glucose electrode, said GDH-based glucose electrode and said reference electrode are within said sample chamber.
a laminated body having a fluid sample inlet end and an electrical contact end;
a fluid sample inlet;
a substantially flat sample chamber in communication between said fluid sample inlet and a vent opening, said test chamber being adapted to collect a fluid sample through said fluid sample inlet;
a GOD-based glucose electrode;
a GDH-based glucose electrode; and a reference electrode wherein said GOD-based glucose electrode, said GDH-based glucose electrode and said reference electrode are within said sample chamber.
2. The glucose biosensor of Claim 1 wherein said GOD-based glucose electrode further includes a redox mediator.
3. The glucose biosensor of Claim 2 wherein said GOD-based glucose electrode further includes one or more of a material selected from the group consisting of a binder, a buffer and a surfactant.
4. The glucose biosensor of Claim 1 wherein said GDH-based glucose electrode further includes a redox mediator.
5. The glucose biosensor of Claim 4 wherein said GDH-based glucose electrode further includes one or more of a material selected from the group consisting of a binder, a buffer and a surfactant.
6. A system for more accurately measuring glucose in a sample comprising:
a first glucose-sensing electrode incorporating a quantity of glucose oxidase;
a second glucose-sensing electrode incorporating a quantity of PQQ-glucose dehydrogenase;
a reference electrode; and means for selecting between a first glucose measurement made with said first glucose-sensing electrode and a second glucose measurement made with said second glucose-sensing electrode.
a first glucose-sensing electrode incorporating a quantity of glucose oxidase;
a second glucose-sensing electrode incorporating a quantity of PQQ-glucose dehydrogenase;
a reference electrode; and means for selecting between a first glucose measurement made with said first glucose-sensing electrode and a second glucose measurement made with said second glucose-sensing electrode.
7. The system of Claim 6 wherein said first glucose-sensing electrode further includes a redox mediator.
8. The system of Claim 7 wherein said first glucose-sensing electrode further includes one or more of a material selected from the group consisting of a binder, a buffer and a surfactant.
9. The system of Claim 6 wherein said second glucose-sensing electrode further includes a redox mediator.
10. The system of Claim 9 wherein said first glucose-sensing electrode further includes one or more of a material selected from the group consisting of a binder, a buffer and a surfactant.
11. The system of Claim 6 wherein said selecting means includes a system capable of determining the difference between said first glucose measurement and said second glucose measurement and selecting one of said first glucose measurement and said second glucose measurement based on said difference when said difference is compared to a predefined value.
12. A method for determining glucose concentration in a blood sample more accurately, said method comprising:
making a first measurement of said glucose concentration of said blood sample using a glucose electrode incorporating glucose oxidase;
making a second measurement of said glucose concentration of said blood sample using a glucose electrode incorporating PQQ-glucose dehydrogenase;
calculating the difference between said first measurement and said second measurement; and selecting one of said first measurement and said second measurement based on said calculated difference.
making a first measurement of said glucose concentration of said blood sample using a glucose electrode incorporating glucose oxidase;
making a second measurement of said glucose concentration of said blood sample using a glucose electrode incorporating PQQ-glucose dehydrogenase;
calculating the difference between said first measurement and said second measurement; and selecting one of said first measurement and said second measurement based on said calculated difference.
13. The method of Claim 12 wherein said selecting step further includes selecting one of said first measurement and said second measurement based on said difference when said difference is compared to a predefined value.
14. The method of Claim 13 wherein said selecting step further includes selecting said first measurement if said calculated difference is greater than about 24 mg/dL.
15. The method of Claim 13 wherein said selecting step further includes selecting said second measurement if said calculated difference is less than or equal to about 24 mg/dL.
16. A disposable glucose sensor comprising:
a sensor body having an open well forming a test chamber;
a GOD-based glucose electrode, a GDH-based glucose electrode and reference/counter electrode disposed within said open well; and electrical contacts on one end of said sensor body.
a sensor body having an open well forming a test chamber;
a GOD-based glucose electrode, a GDH-based glucose electrode and reference/counter electrode disposed within said open well; and electrical contacts on one end of said sensor body.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11/306,005 | 2005-12-14 | ||
US11/306,005 US7955484B2 (en) | 2005-12-14 | 2005-12-14 | Glucose biosensor and method |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2568824A1 true CA2568824A1 (en) | 2007-06-14 |
CA2568824C CA2568824C (en) | 2010-06-08 |
Family
ID=37907140
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2568824A Active CA2568824C (en) | 2005-12-14 | 2006-11-24 | Glucose biosensor and method |
Country Status (9)
Country | Link |
---|---|
US (1) | US7955484B2 (en) |
EP (1) | EP1798289B1 (en) |
JP (1) | JP4463266B2 (en) |
KR (1) | KR100790141B1 (en) |
CN (1) | CN1991368B (en) |
AT (1) | ATE414166T1 (en) |
CA (1) | CA2568824C (en) |
DE (1) | DE602006003615D1 (en) |
ES (1) | ES2317407T3 (en) |
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2006
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- 2006-11-24 ES ES06024459T patent/ES2317407T3/en active Active
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- 2006-11-24 DE DE602006003615T patent/DE602006003615D1/en active Active
- 2006-11-24 CA CA2568824A patent/CA2568824C/en active Active
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JP2007163499A (en) | 2007-06-28 |
KR100790141B1 (en) | 2008-01-02 |
ATE414166T1 (en) | 2008-11-15 |
CA2568824C (en) | 2010-06-08 |
EP1798289B1 (en) | 2008-11-12 |
JP4463266B2 (en) | 2010-05-19 |
EP1798289A1 (en) | 2007-06-20 |
US20070131549A1 (en) | 2007-06-14 |
US7955484B2 (en) | 2011-06-07 |
ES2317407T3 (en) | 2009-04-16 |
CN1991368B (en) | 2012-01-25 |
DE602006003615D1 (en) | 2008-12-24 |
CN1991368A (en) | 2007-07-04 |
KR20070063430A (en) | 2007-06-19 |
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