CA2499049A1 - Method of making sensor electrodes - Google Patents

Method of making sensor electrodes Download PDF

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Publication number
CA2499049A1
CA2499049A1 CA002499049A CA2499049A CA2499049A1 CA 2499049 A1 CA2499049 A1 CA 2499049A1 CA 002499049 A CA002499049 A CA 002499049A CA 2499049 A CA2499049 A CA 2499049A CA 2499049 A1 CA2499049 A1 CA 2499049A1
Authority
CA
Canada
Prior art keywords
conducting material
electrically conducting
substrate
affixed
case
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002499049A
Other languages
French (fr)
Other versions
CA2499049C (en
Inventor
Eric R. Diebold
Richard J. Kordal
Nigel A. Surridge
Christopher D. Wilsey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics Operations Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2499049A1 publication Critical patent/CA2499049A1/en
Application granted granted Critical
Publication of CA2499049C publication Critical patent/CA2499049C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/817—Enzyme or microbe electrode

Abstract

A method for fabricating high-resolution, biocompatible electrodes is disclosed, allowing production of an electrochemical sensor which is capable of precise analyte concentration determination on a very small sample size. Electrically conducting material is affixed to a first insulating substrate. A second insulating substrate is then affixed to the electrically conducting material and patterned using photolithography to define an electrode area.
Alternatively, the electrically conducting material may be screen printed directly onto a standard printed circuit board substrate in the case of a counter or reference electrode. In either case, the substrate may be rigid or flexible. When the electrodes produced in accordance with the present invention are then used in an electrochemical sensor which includes a reagent, the small and highly-defined electrode areas permit highly-accurate electrochemical analyte measurements to be performed on very small sample sizes.
CA002499049A 1994-02-22 1995-02-21 Method of making sensor electrodes Expired - Fee Related CA2499049C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/200,174 1994-02-22
US08/200,174 US5437999A (en) 1994-02-22 1994-02-22 Electrochemical sensor
CA002183865A CA2183865C (en) 1994-02-22 1995-02-21 Method of making sensor electrodes

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA002183865A Division CA2183865C (en) 1994-02-22 1995-02-21 Method of making sensor electrodes

Publications (2)

Publication Number Publication Date
CA2499049A1 true CA2499049A1 (en) 1995-08-24
CA2499049C CA2499049C (en) 2006-07-11

Family

ID=22740645

Family Applications (3)

Application Number Title Priority Date Filing Date
CA002499867A Abandoned CA2499867A1 (en) 1994-02-22 1995-02-21 Method of making sensor electrodes
CA002499049A Expired - Fee Related CA2499049C (en) 1994-02-22 1995-02-21 Method of making sensor electrodes
CA002183865A Expired - Fee Related CA2183865C (en) 1994-02-22 1995-02-21 Method of making sensor electrodes

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA002499867A Abandoned CA2499867A1 (en) 1994-02-22 1995-02-21 Method of making sensor electrodes

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA002183865A Expired - Fee Related CA2183865C (en) 1994-02-22 1995-02-21 Method of making sensor electrodes

Country Status (8)

Country Link
US (1) US5437999A (en)
EP (1) EP0753051B1 (en)
JP (1) JP3193721B2 (en)
CA (3) CA2499867A1 (en)
DE (1) DE69519725T2 (en)
ES (1) ES2154330T3 (en)
MX (1) MX9603543A (en)
WO (1) WO1995022597A1 (en)

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