CA2383791A1 - Methods of modification of selected cells in a magnetic cell separation column - Google Patents

Methods of modification of selected cells in a magnetic cell separation column Download PDF

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Publication number
CA2383791A1
CA2383791A1 CA002383791A CA2383791A CA2383791A1 CA 2383791 A1 CA2383791 A1 CA 2383791A1 CA 002383791 A CA002383791 A CA 002383791A CA 2383791 A CA2383791 A CA 2383791A CA 2383791 A1 CA2383791 A1 CA 2383791A1
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CA
Canada
Prior art keywords
selected cells
column
modifying
cells
retained
Prior art date
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Granted
Application number
CA002383791A
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French (fr)
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CA2383791C (en
Inventor
Stefan Miltenyi
Mario Assenmacher
Jurgen Schmitz
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Miltenyi Biotec GmbH
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Individual
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Publication of CA2383791A1 publication Critical patent/CA2383791A1/en
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Publication of CA2383791C publication Critical patent/CA2383791C/en
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Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/002—High gradient magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/025—High gradient magnetic separators
    • B03C1/031—Component parts; Auxiliary operations
    • B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
    • B03C1/034—Component parts; Auxiliary operations characterised by the magnetic circuit characterised by the matrix elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/806—Electrical property or magnetic property

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  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

A method is provided for modifying cells retained in a high gradient magneti c cell separation column (HGMS). Selected cells within a heterogeneous mixture are labeled with a label comprising a magnetic particle and specific for the selected cells. The labeled, selected cells are applied to the magnetic cell separation column, which retains the selected cells. The selected cells are then modified while retained in the column and then removed to the original suspension or to a purified, homogeneous suspension.

Claims (23)

1. A method for modifying selected cells comprising modifying magnetically labeled selected cells retained on a high gradient magnetic separation column thereby producing modified, selected cells.
2. A method for modifying selected cells comprising the steps of (a) applying a population of cells to a high gradient magnetic separation column wherein said population of cells comprises magnetically labeled selected cells and wherein said magnetically labeled selected cells are retained by said column; and (b) modifying said selected cells retained by said column thereby producing modified, selected cells.
3. The method of claim 1 further comprising the step of removing the modified, selected cells from the column.
4. The method of claim 2 further comprising the step of removing the modified, selected cells from the column.
5. The method according to claim 1, wherein the high gradient magnetic cell separation column contains a high volume of a matrix relative to the total volume of the column.
6. The method of claim 5, wherein the volume of the matrix is more than 50% of the total volume of the column.
7. The method of claim 5, wherein the volume of the matrix is more than 60% of the total volume of the column.
8. The method of claim 5, wherein the matrix comprises ferromagnetic spheres.
9. The method of claim 3 wherein said removing is by removing said magnetic field.
10. The method of claim 1, wherein said modifying comprises intracellular staining of said selected cells.
11. The method of claim 1, wherein said modifying comprises permeabilizing said selected cells.
12. The method of claim 1, wherein said modifying comprises a second labeling of said selected cells.
13. The method of claim 1, wherein said modifying comprises binding a biologically reactive compound to the selected cells.
14. The method of claim 13 wherein said biologically reactive compound includes antibodies, ligands, proteins, peptides, nucleic acids, polynucleotides, oligonucleotides, lectins, lipids or enzymes.
15. The method of claim 1, wherein said modifying comprises transfecting the selected cells with an expression vector.
16. The method of claim 1 wherein said modifying comprises applying an enzyme to said selected cells.
17. The method of claim 1 wherein said modifying comprises applying a pharmacological agent to said selected cells.
18. The method of claim 1 wherein said modifying comprises applying a biological modifier to said selected cells.
19. The method of claim 1 wherein said modifying comprises applying a chemical agent to said selected cells.
20. The method of claim 1, wherein said modifying comprises applying a first and a second modifying agent to said selected cells.
21. The method of claim 3, further comprising applying the removed, selected cells to a second high gradient magnetic cell separation column such that the selected cells are retained by said second column; and modifying the selected cells retained by said second column.
22. The method of claim 21 further comprising the step of removing said selected cells from said second column.
23. The method of claim 8 wherein said ferromagnetic spheres are coated with a biocompatible polymer.
CA2383791A 1999-09-03 2000-09-01 Methods of modification of selected cells in a magnetic cell separation column Expired - Lifetime CA2383791C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15240899P 1999-09-03 1999-09-03
US60/152,408 1999-09-03
PCT/IB2000/001739 WO2001017687A2 (en) 1999-09-03 2000-09-01 Methods of modification of selected cells in a magnetic cell separation column

Publications (2)

Publication Number Publication Date
CA2383791A1 true CA2383791A1 (en) 2001-03-15
CA2383791C CA2383791C (en) 2012-11-06

Family

ID=22542799

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2383791A Expired - Lifetime CA2383791C (en) 1999-09-03 2000-09-01 Methods of modification of selected cells in a magnetic cell separation column

Country Status (9)

Country Link
US (1) US6468432B1 (en)
EP (1) EP1207961B1 (en)
JP (1) JP4612982B2 (en)
AT (1) ATE320314T1 (en)
AU (1) AU778569B2 (en)
CA (1) CA2383791C (en)
DE (1) DE60026729T2 (en)
ES (1) ES2260063T3 (en)
WO (1) WO2001017687A2 (en)

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US6649419B1 (en) * 2000-11-28 2003-11-18 Large Scale Proteomics Corp. Method and apparatus for protein manipulation
US20030064359A1 (en) * 2001-09-21 2003-04-03 Joel Lanoix Preparation of highly-purified plasma membranes
US20030099954A1 (en) * 2001-11-26 2003-05-29 Stefan Miltenyi Apparatus and method for modification of magnetically immobilized biomolecules
US20040142384A1 (en) * 2003-01-16 2004-07-22 Cohen Barb Ariel Magnetic separator
DE10362104B4 (en) * 2003-08-29 2008-02-14 Kist-Europe Forschungsgesellschaft Mbh Cell processor for disease treatment
WO2005065267A2 (en) * 2003-12-24 2005-07-21 Massachusetts Institute Of Technology Magnetophoretic cell clarification
GB2425498A (en) * 2005-04-25 2006-11-01 Dynal Biotech Asa A magnetic separation device
US20090208981A1 (en) * 2005-05-03 2009-08-20 Jia-Ming Chang Method for Analysis of Interaction Between Small Molecules and Cells and Apparatus thereof
US20090081750A1 (en) * 2007-02-14 2009-03-26 Bio-Rad Laboratories, Inc. Transfection in magnetically driven continuous flow
US8071395B2 (en) * 2007-12-12 2011-12-06 The Board Of Trustees Of The Leland Stanford Junior University Methods and apparatus for magnetic separation of cells
KR101384142B1 (en) * 2007-12-28 2014-04-14 삼성디스플레이 주식회사 Display substrate, method for manufacturing the display substrate and display apparatus having the display substrate
US8790916B2 (en) 2009-05-14 2014-07-29 Genestream, Inc. Microfluidic method and system for isolating particles from biological fluid
JP5678565B2 (en) 2009-12-08 2015-03-04 Jnc株式会社 Magnetic fine particles and method for producing the same
DE102010042723A1 (en) * 2010-10-20 2012-04-26 Miltenyi Biotec Gmbh Apparatus and method for separating Neél and Brown magnetic particles
US8695618B2 (en) 2010-12-22 2014-04-15 Carnegie Mellon University 3D chemical pattern control in 2D fluidics devices
EP2518504A1 (en) * 2011-04-29 2012-10-31 Miltenyi Biotec GmbH A method for the quantitative and qualitative characterization of antigen-specific T cells recognizing a specific antigen
EP2734307B1 (en) * 2011-07-21 2020-03-25 Menarini Silicon Biosystems S.p.A. Assay to capture and detect circulating multiple myeloma cells from blood
EP3037170A1 (en) * 2014-12-27 2016-06-29 Miltenyi Biotec GmbH Multisort cell separation method
EP3247808B1 (en) 2015-01-21 2021-05-05 Fred Hutchinson Cancer Research Center Point-of-care and/or portable platform for gene therapy
CN109459571A (en) * 2018-11-08 2019-03-12 浙江大学 Based on micro-fluidic and chemiluminescence immune assay superparamagnetic nanoparticle excretion body separation detecting system and its application

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DE68919715T2 (en) 1988-12-28 1995-04-06 Stefan Miltenyi METHOD AND MATERIALS FOR HIGHLY GRADUATED MAGNETIC SPLITTING OF BIOLOGICAL MATERIALS.
US5200084A (en) 1990-09-26 1993-04-06 Immunicon Corporation Apparatus and methods for magnetic separation
WO1994011078A1 (en) 1992-11-16 1994-05-26 Immunivest Corporation Magnetic immobilization and manipulation of biological entities
US5622831A (en) * 1990-09-26 1997-04-22 Immunivest Corporation Methods and devices for manipulation of magnetically collected material
ES2191677T3 (en) * 1992-10-21 2003-09-16 Stefan Miltenyi DIRECT SELECTION OF CELLS THROUGH A SECRETION PRODUCT.
US5514340A (en) * 1994-01-24 1996-05-07 Magnetix Biotechnology, Inc. Device for separating magnetically labelled cells
GB9413029D0 (en) 1994-06-29 1994-08-17 Common Services Agency Stem cell immobilisation
US5705059A (en) 1995-02-27 1998-01-06 Miltenyi; Stefan Magnetic separation apparatus
US6190870B1 (en) * 1995-08-28 2001-02-20 Amcell Corporation Efficient enrichment and detection of disseminated tumor cells
GB9523045D0 (en) * 1995-11-10 1996-01-10 Genera Tech Ltd Methods of capture and asssy procedures
US6228624B1 (en) * 1996-08-02 2001-05-08 Immunivest Corporation Method to select and transfect cell subpopulations
JPH10179165A (en) * 1996-12-27 1998-07-07 Norin Suisansyo Chikusan Shikenjo Separation and purification of reproductive stem cell using antibody magnetic beads and establishment of stem cell strain.

Also Published As

Publication number Publication date
ATE320314T1 (en) 2006-04-15
EP1207961B1 (en) 2006-03-15
AU778569B2 (en) 2004-12-09
US6468432B1 (en) 2002-10-22
CA2383791C (en) 2012-11-06
AU1294801A (en) 2001-04-10
JP4612982B2 (en) 2011-01-12
DE60026729T2 (en) 2006-12-14
EP1207961A2 (en) 2002-05-29
WO2001017687A2 (en) 2001-03-15
DE60026729D1 (en) 2006-05-11
JP2003508073A (en) 2003-03-04
WO2001017687A3 (en) 2002-01-17
ES2260063T3 (en) 2006-11-01

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