CA2356364A1 - Cholesterol sensor and method for determining cholesterol - Google Patents

Cholesterol sensor and method for determining cholesterol Download PDF

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Publication number
CA2356364A1
CA2356364A1 CA002356364A CA2356364A CA2356364A1 CA 2356364 A1 CA2356364 A1 CA 2356364A1 CA 002356364 A CA002356364 A CA 002356364A CA 2356364 A CA2356364 A CA 2356364A CA 2356364 A1 CA2356364 A1 CA 2356364A1
Authority
CA
Canada
Prior art keywords
cholesterol
reagent
sensor
low density
density lipoprotein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002356364A
Other languages
French (fr)
Other versions
CA2356364C (en
Inventor
Motokazu Watanabe
Keiko Yugawa
Shiro Nankai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panasonic Holdings Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2356364A1 publication Critical patent/CA2356364A1/en
Application granted granted Critical
Publication of CA2356364C publication Critical patent/CA2356364C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/817—Enzyme or microbe electrode

Abstract

The present invention provides a sensor that electrochemically determines cholesterol in low density lipoprotein by only one feed of a sample. The sensor has:
an electrode system that is mounted on an electrically insulating base plate and includes at least a working electrode and a counter electrode; an enzyme layer formed on the base plate with the electrode system; and a reagent layer that is arranged before the enzyme layer in a sample solution supply path to the electrode system. The enzyme layer includes at least an oxidoreductase and an electron mediator. The reagent layer includes a reagent that depresses reactivity of cholesterol in lipoproteins other than the low density lipoprotein with the oxidoreductase, for example, a reagent that attaches to lipoproteins other than the low density lipoprotein to form a water-soluble complex.
CA002356364A 1999-11-22 2000-11-16 Cholesterol sensor and method for determining cholesterol Expired - Fee Related CA2356364C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP33121999 1999-11-22
JP11/331219 1999-11-22
PCT/JP2000/008100 WO2001038862A1 (en) 1999-11-22 2000-11-16 Cholesterol sensor and method for determining cholesterol

Publications (2)

Publication Number Publication Date
CA2356364A1 true CA2356364A1 (en) 2001-05-31
CA2356364C CA2356364C (en) 2003-12-09

Family

ID=18241237

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002356364A Expired - Fee Related CA2356364C (en) 1999-11-22 2000-11-16 Cholesterol sensor and method for determining cholesterol

Country Status (7)

Country Link
US (1) US6342364B1 (en)
EP (1) EP1150118B1 (en)
JP (1) JP4646475B2 (en)
CN (1) CN1187609C (en)
CA (1) CA2356364C (en)
DE (1) DE60018039T2 (en)
WO (1) WO2001038862A1 (en)

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US7645421B2 (en) 2003-06-20 2010-01-12 Roche Diagnostics Operations, Inc. System and method for coding information on a biosensor test strip
US8206565B2 (en) 2003-06-20 2012-06-26 Roche Diagnostics Operation, Inc. System and method for coding information on a biosensor test strip
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US8058077B2 (en) 2003-06-20 2011-11-15 Roche Diagnostics Operations, Inc. Method for coding information on a biosensor test strip
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US7645373B2 (en) * 2003-06-20 2010-01-12 Roche Diagnostic Operations, Inc. System and method for coding information on a biosensor test strip
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CN101001946B (en) * 2004-02-03 2015-07-22 聚合物技术系统公司 Reagent combination and method for direct test strip measurement of cholesterol from low density lipoproteins at ambient temperatures
US7625721B2 (en) 2004-02-03 2009-12-01 Polymer Technology Systems, Inc. Non-precipitating bodily fluid analysis system
US20060062688A1 (en) * 2004-02-03 2006-03-23 Polymer Technology Systems, Inc. Bodily fluid analysis system
BRPI0507376A (en) 2004-02-06 2007-07-10 Bayer Healthcare Llc oxidizable species as an internal reference for biosensors and method of use
US7556723B2 (en) * 2004-06-18 2009-07-07 Roche Diagnostics Operations, Inc. Electrode design for biosensor
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CN101151526B (en) * 2005-03-29 2012-05-30 Cci株式会社 Biosensor
CN103558284B (en) 2005-07-20 2017-04-12 安晟信医疗科技控股公司 Gated amperometry
US8187298B2 (en) * 2005-08-04 2012-05-29 Cook Medical Technologies Llc Embolic protection device having inflatable frame
CN101273266B (en) 2005-09-30 2012-08-22 拜尔健康护理有限责任公司 Gated voltammetry
GB0526051D0 (en) 2005-12-21 2006-02-01 Oxford Biosensors Ltd Cholesterol sensor
CN100403018C (en) * 2006-01-20 2008-07-16 南京剑桥医疗器械有限公司 Siphon nano-biosensing chip and preparing method thereof
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US9632080B2 (en) 2009-01-23 2017-04-25 Polymer Technology Systems, Inc. Diagnostic multi-layer dry phase test strip with integrated biosensors (“electrostrip”)
JP5285000B2 (en) * 2010-02-23 2013-09-11 富士フイルム株式会社 Method for measuring low density lipoprotein cholesterol
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RU2014148800A (en) * 2012-05-04 2016-06-27 Полимер Текнолоджи Системз, Инк. SYSTEMS AND METHODS FOR DETERMINING LDL CHOLESTEROL IS NOT A NATO
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Also Published As

Publication number Publication date
EP1150118B1 (en) 2005-02-09
EP1150118A4 (en) 2003-01-08
CA2356364C (en) 2003-12-09
CN1187609C (en) 2005-02-02
DE60018039D1 (en) 2005-03-17
US6342364B1 (en) 2002-01-29
JP4646475B2 (en) 2011-03-09
EP1150118A1 (en) 2001-10-31
DE60018039T2 (en) 2005-09-29
WO2001038862A1 (en) 2001-05-31
CN1337005A (en) 2002-02-20

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EEER Examination request
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Effective date: 20121116