CA2337490A1 - Identification of compound-protein interactions using libraries of protein-nucleic acid fusion molecules - Google Patents
Identification of compound-protein interactions using libraries of protein-nucleic acid fusion molecules Download PDFInfo
- Publication number
- CA2337490A1 CA2337490A1 CA002337490A CA2337490A CA2337490A1 CA 2337490 A1 CA2337490 A1 CA 2337490A1 CA 002337490 A CA002337490 A CA 002337490A CA 2337490 A CA2337490 A CA 2337490A CA 2337490 A1 CA2337490 A1 CA 2337490A1
- Authority
- CA
- Canada
- Prior art keywords
- protein
- compound
- fusion
- nucleic acid
- solid support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000004927 fusion Effects 0.000 title claims abstract 24
- 230000003993 interaction Effects 0.000 title claims abstract 5
- 150000001875 compounds Chemical class 0.000 claims abstract 18
- 239000007787 solid Substances 0.000 claims abstract 12
- 108090000623 proteins and genes Proteins 0.000 claims abstract 10
- 102000004169 proteins and genes Human genes 0.000 claims abstract 10
- 108020004707 nucleic acids Proteins 0.000 claims abstract 4
- 102000039446 nucleic acids Human genes 0.000 claims abstract 4
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 4
- 230000015572 biosynthetic process Effects 0.000 claims abstract 2
- 239000011324 bead Substances 0.000 claims 4
- 150000003384 small molecules Chemical class 0.000 claims 2
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 239000011521 glass Substances 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
- C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
Abstract
Disclosed herein is a method for detecting a compound-protein interaction, involving: (a) providing a compound library in which each member of the compound library is immobilized on a solid support; (b) contacting each member of the immobilized compound library in a single reaction chamber with each member of a protein-nucleic acid fusion library under conditions which allow the formation of compound-fusion complexes; (c) isolating the immobilized compound-fusion complexes; and (d) detecting a compound-fusion complex as an indication that the protein of the fusion interacts with the compound. In preferred embodiments, the protein is identified by reading the nucleic acid portion of the fusion, and the compound is identified by reading a detectable tag bound to either the compound or the solid support.
Claims (20)
1. A method for detecting a compound-protein interaction, said method comprising:
(a) providing a compound library in which each member of said compound library is immobilized on a solid support;
(b) contacting each member of said immobilized compound library in a single reaction chamber with each member of a protein-nucleic acid fusion library under conditions which allow the formation of compound-fusion complexes;
(c) isolating said immobilized compound-fusion complexes; and (d) detecting said compound-fusion complex as an indication that the protein of said fusion interacts with said compound.
(a) providing a compound library in which each member of said compound library is immobilized on a solid support;
(b) contacting each member of said immobilized compound library in a single reaction chamber with each member of a protein-nucleic acid fusion library under conditions which allow the formation of compound-fusion complexes;
(c) isolating said immobilized compound-fusion complexes; and (d) detecting said compound-fusion complex as an indication that the protein of said fusion interacts with said compound.
2. The method of claim 1, wherein said protein-nucleic acid fusion is a protein-RNA fusion, a protein-DNA fusion, or a protein fused to a DNA-RNA hybrid molecule.
3. The method of claim 1, wherein said solid support is a bead.
4. The method of claim 3, wherein each said bead is coded with a unique detectable label.
5. The method of claim 4, wherein the compound of said complexed protein-nucleic acid fusion is identified by said unique detectable label associated with said bead.
6. The method of claim 4, wherein said detectable label is a peptide label, a nucleic acid label, a chemical label, a fluorescent label, or a radio frequency tag.
7. The method of claim 1, wherein said solid support is a chip and said compound library is immobilized on said chip in an addressable array.
8. The method of claim 7, wherein each member of said protein-nucleic acid fusion library is detectably labeled.
9. The method of claim 1, wherein said compound-fusion complex, or the components thereof, are recovered by release from said solid support.
10. The method of claim 1, wherein said method further comprises recovering said protein-nucleic acid fusion from said solid support and identifying said protein.
11. The method of claim 10, wherein the identity of said protein is determined from the sequence of the nucleic acid portion of said protein-nucleic acid fusion.
12. The method of claim 1, wherein said compound is a small molecule.
13. A method for detecting a compound-protein interaction, said method comprising:
(a) providing a compound immobilized on a solid support;
(b) contacting said immobilized compound with a protein-nucleic acid fusion library under conditions which allow said fusion to bind to said compound; and (c) detecting a bound protein-nucleic acid fusion as an indication that the protein of said protein-nucleic acid fusion interacts with said compound.
(a) providing a compound immobilized on a solid support;
(b) contacting said immobilized compound with a protein-nucleic acid fusion library under conditions which allow said fusion to bind to said compound; and (c) detecting a bound protein-nucleic acid fusion as an indication that the protein of said protein-nucleic acid fusion interacts with said compound.
14. The method of claim 13, wherein said protein-nucleic acid fusion is a protein-RNA fusion, a protein-DNA fusion, or a protein fused to a DNA-RNA hybrid molecule.
15. The method of claim 13, wherein said protein-nucleic acid fusion is detectably labeled and said interaction is indicated by the association of said detectable label with said solid support.
16. The method of claim 13, wherein said bound protein-nucleic acid fusion is recovered by release from said solid support.
17. The method of claim 13, wherein said method further comprises recovering said protein-nucleic acid fusion from said solid support and identifying said protein.
18. The method of claim 17, wherein the identity of said protein is determined from the sequence of the nucleic acid portion of said protein-nucleic acid fusion.
19. The method of claim 13, wherein said solid support is a column, glass slide, chip, or bead.
20. The method of claim 13, wherein said compound is a small molecule.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US9682098P | 1998-08-17 | 1998-08-17 | |
US60/096,820 | 1998-08-17 | ||
PCT/US1999/018600 WO2000009464A1 (en) | 1998-08-17 | 1999-08-16 | Identification of compound-protein interactions using libraries of protein-nucleic acid fusion molecules |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2337490A1 true CA2337490A1 (en) | 2000-02-24 |
CA2337490C CA2337490C (en) | 2012-01-03 |
Family
ID=22259237
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2337490A Expired - Lifetime CA2337490C (en) | 1998-08-17 | 1999-08-16 | Identification of compound-protein interactions using libraries of protein-nucleic acid fusion molecules |
Country Status (12)
Country | Link |
---|---|
US (1) | US6602685B1 (en) |
EP (1) | EP1105360B1 (en) |
JP (1) | JP4278872B2 (en) |
AT (1) | ATE437844T1 (en) |
AU (1) | AU757955B2 (en) |
CA (1) | CA2337490C (en) |
CY (1) | CY1110515T1 (en) |
DE (1) | DE69941193D1 (en) |
DK (1) | DK1105360T3 (en) |
ES (1) | ES2329205T3 (en) |
PT (1) | PT1105360E (en) |
WO (1) | WO2000009464A1 (en) |
Families Citing this family (44)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6517781B1 (en) * | 1997-06-02 | 2003-02-11 | Aurora Biosciences Corporation | Low fluorescence assay platforms and related methods for drug discovery |
CA2382545C (en) * | 1999-08-27 | 2013-08-13 | Phylos, Inc. | Methods for encoding and sorting in vitro translated proteins |
US7022479B2 (en) | 2000-01-24 | 2006-04-04 | Compound Therapeutics, Inc. | Sensitive, multiplexed diagnostic assays for protein analysis |
JP2003520050A (en) | 2000-01-24 | 2003-07-02 | フィロス インク. | Sensitive multiplexed diagnostic assays for protein analysis |
GB2360282A (en) * | 2000-03-17 | 2001-09-19 | Bioinvent Int Ab | Making and using micro-arrays of biological materials |
US6962781B1 (en) | 2000-05-19 | 2005-11-08 | Proteonova, Inc. | In vitro evolution of nucleic acids and encoded polypeptide |
US7410761B2 (en) * | 2000-05-19 | 2008-08-12 | Proteonova, Inc. | System for rapid identification and selection of nucleic acids and polypeptides, and method thereof |
US20040229271A1 (en) | 2000-05-19 | 2004-11-18 | Williams Richard B. | Compositions and methods for the identification and selection of nucleic acids and polypeptides |
US6539102B1 (en) | 2000-09-01 | 2003-03-25 | Large Scale Proteomics | Reference database |
US6980674B2 (en) | 2000-09-01 | 2005-12-27 | Large Scale Proteomics Corp. | Reference database |
WO2003029490A1 (en) * | 2001-10-01 | 2003-04-10 | Yingjian Wang | Methods and arrays for detecting biological molecules |
US7125669B2 (en) * | 2001-11-27 | 2006-10-24 | Compound Therapeutics, Inc. | Solid-phase immobilization of proteins and peptides |
AU2003267951A1 (en) * | 2002-04-26 | 2003-12-19 | Genencor International, Inc. | Methods and articles for high throughput analysis of biomolecular interactions |
JPWO2003097862A1 (en) * | 2002-05-17 | 2005-09-15 | 協和醗酵工業株式会社 | Method for searching for substance having antidiabetic action |
US7927791B2 (en) | 2002-07-24 | 2011-04-19 | Ptc Therapeutics, Inc. | Methods for identifying small molecules that modulate premature translation termination and nonsense mediated mRNA decay |
US20040142379A1 (en) * | 2003-01-16 | 2004-07-22 | Carlsberg Research Laboratory | Affinity fishing for ligands and proteins receptors |
CA2546343A1 (en) * | 2003-11-20 | 2005-06-09 | Sanofi Pasteur, Inc. | Methods for purifying pertussis toxin and peptides useful therefor |
WO2007037706A2 (en) * | 2005-09-27 | 2007-04-05 | Bernd Helmut Adam Rehm | Polymer particles and uses thereof |
CA2626522A1 (en) | 2005-11-16 | 2007-05-24 | Ambrx, Inc. | Methods and compositions comprising non-natural amino acids |
US7749957B2 (en) | 2006-04-06 | 2010-07-06 | E.I. Du Pont De Nemours And Company | Clay-binding peptides and methods of use |
WO2009009186A2 (en) * | 2007-04-13 | 2009-01-15 | The Regents Of The University Of California | Receptors useful for gas phase chemical sensing |
US7951559B2 (en) * | 2007-07-25 | 2011-05-31 | E.I. Du Pont De Nemours And Company | Recombinant peptide production using a cross-linkable solubility tag |
US7829311B2 (en) | 2007-07-25 | 2010-11-09 | E.I. Du Pont De Nemours And Company | Ketosteroid isomerase inclusion body tag engineered to be acid-resistant by replacing aspartates with glutamate |
US7678883B2 (en) * | 2007-07-25 | 2010-03-16 | E.I. Du Pont De Nemours And Company | Solubility tags for the expression and purification of bioactive peptides |
US7794963B2 (en) | 2007-11-02 | 2010-09-14 | E.I. Du Pont De Nemours And Company | Use of tetracysteine tags in fluorescence-activated cell sorting analysis of prokaryotic cells producing peptides or proteins |
US8883146B2 (en) | 2007-11-30 | 2014-11-11 | Abbvie Inc. | Protein formulations and methods of making same |
AU2008334099B2 (en) | 2007-11-30 | 2014-07-24 | Abbvie Biotechnology Ltd. | Protein formulations and methods of making same |
EP3629022A1 (en) | 2008-07-25 | 2020-04-01 | Richard W. Wagner | Protein screening methods |
US20100158822A1 (en) * | 2008-12-18 | 2010-06-24 | E .I. Du Pont De Nemours And Company | Peptides that bind to silica-coated particles |
US20100158837A1 (en) | 2008-12-18 | 2010-06-24 | E. I. Du Pont De Nemours And Company | Iron oxide-binding peptides |
US20100158846A1 (en) * | 2008-12-18 | 2010-06-24 | E. I. Du Pont De Nemours And Company | Hair-binding peptides |
US8287845B2 (en) * | 2008-12-18 | 2012-10-16 | E I Du Pont De Nemours And Company | Hair-binding peptides |
US8481678B2 (en) | 2009-03-30 | 2013-07-09 | E I Du Pont De Nemours And Company | Peptide-based tooth whitening reagents |
US20100247590A1 (en) * | 2009-03-30 | 2010-09-30 | Johnson & Johnson | Peptide-Based Systems For Delivery Of Cosmetic Agents |
MX2012009318A (en) | 2010-02-10 | 2012-09-07 | Novartis Ag | Methods and compounds for muscle growth. |
DE102010056289A1 (en) | 2010-12-24 | 2012-06-28 | Geneart Ag | Process for the preparation of reading frame correct fragment libraries |
ES2873375T3 (en) | 2011-03-15 | 2021-11-03 | X Body Inc | Antibody screening methods |
JP6153921B2 (en) | 2011-03-24 | 2017-06-28 | オプコ ファーマシューティカルズ、エルエルシー | Biomarker discovery in liquid biological samples using bead or particle-based libraries, and diagnostic kits and treatments |
KR102151656B1 (en) | 2011-04-28 | 2020-09-03 | 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 | Identification of polynucleotides associated with a sample |
EP2788379B1 (en) | 2011-12-05 | 2020-02-05 | X-Body, Inc. | Pdgf receptor beta binding polypeptides |
US9938523B2 (en) * | 2013-03-15 | 2018-04-10 | Arizona Board Of Regents On Behalf Of Arizona State University | Nucleic acid-tagged compositions and methods for multiplexed protein-protein interaction profiling |
RU2678421C2 (en) | 2013-06-28 | 2019-01-28 | Экс-Боди, Инк. | Target antigen discovery, phenotypic screens and use thereof for identification of target cell specific target epitopes |
EP3049811B1 (en) | 2013-09-23 | 2020-09-09 | X-Body, Inc. | Methods and compositions for generation of binding agents against cell surface antigens |
WO2015120058A2 (en) | 2014-02-05 | 2015-08-13 | Molecular Templates, Inc. | Methods of screening, selecting, and identifying cytotoxic recombinant polypeptides based on an interim diminution of ribotoxicity |
Family Cites Families (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4537861A (en) * | 1983-02-03 | 1985-08-27 | Elings Virgil B | Apparatus and method for homogeneous immunoassay |
US4587044A (en) | 1983-09-01 | 1986-05-06 | The Johns Hopkins University | Linkage of proteins to nucleic acids |
CH0229046H1 (en) | 1985-03-30 | 1998-07-15 | Stuart Alan Kauffman | METHOD FOR OBTAINING DNA, RNA, PEPTIDES, POLYPEPTINIQUE. DES OR PROTEINS BY MEANS OF A DNA RECOMBINANT TECH |
US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
US5424186A (en) | 1989-06-07 | 1995-06-13 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis |
EP0494955B1 (en) | 1989-10-05 | 1998-07-15 | Optein, Inc. | Cell-free synthesis and isolation of novel genes and polypeptides |
US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
US5723286A (en) | 1990-06-20 | 1998-03-03 | Affymax Technologies N.V. | Peptide library and screening systems |
US5650489A (en) * | 1990-07-02 | 1997-07-22 | The Arizona Board Of Regents | Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof |
US5843701A (en) * | 1990-08-02 | 1998-12-01 | Nexstar Pharmaceticals, Inc. | Systematic polypeptide evolution by reverse translation |
AU8498091A (en) | 1990-08-02 | 1992-03-02 | Regents Of The University Of Colorado, The | Systematic polypeptide evolution by reverse translation |
US5264563A (en) | 1990-08-24 | 1993-11-23 | Ixsys Inc. | Process for synthesizing oligonucleotides with random codons |
DE4112440C1 (en) | 1991-04-16 | 1992-10-22 | Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De | |
US5795747A (en) | 1991-04-16 | 1998-08-18 | Evotec Biosystems Gmbh | Process for manufacturing new biopolymers |
AU2313392A (en) | 1991-08-01 | 1993-03-02 | University Research Corporation | Systematic polypeptide evolution by reverse translation |
US5639603A (en) | 1991-09-18 | 1997-06-17 | Affymax Technologies N.V. | Synthesizing and screening molecular diversity |
US5270170A (en) | 1991-10-16 | 1993-12-14 | Affymax Technologies N.V. | Peptide library and screening method |
US5733731A (en) | 1991-10-16 | 1998-03-31 | Affymax Technologies N.V. | Peptide library and screening method |
US5541061A (en) | 1992-04-29 | 1996-07-30 | Affymax Technologies N.V. | Methods for screening factorial chemical libraries |
US5565324A (en) | 1992-10-01 | 1996-10-15 | The Trustees Of Columbia University In The City Of New York | Complex combinatorial chemical libraries encoded with tags |
US6087186A (en) | 1993-07-16 | 2000-07-11 | Irori | Methods and apparatus for synthesizing labeled combinatorial chemistry libraries |
US5635602A (en) | 1993-08-13 | 1997-06-03 | The Regents Of The University Of California | Design and synthesis of bispecific DNA-antibody conjugates |
AU8124694A (en) | 1993-10-29 | 1995-05-22 | Affymax Technologies N.V. | In vitro peptide and antibody display libraries |
US5561043A (en) | 1994-01-31 | 1996-10-01 | Trustees Of Boston University | Self-assembling multimeric nucleic acid constructs |
DK0744958T3 (en) | 1994-01-31 | 2003-10-20 | Univ Boston | Polyclonal antibody libraries |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
WO1995032425A1 (en) | 1994-05-23 | 1995-11-30 | Smithkline Beecham Corporation | Encoded combinatorial libraries |
US5627024A (en) | 1994-08-05 | 1997-05-06 | The Scripps Research Institute | Lambdoid bacteriophage vectors for expression and display of foreign proteins |
US5559000A (en) | 1995-01-18 | 1996-09-24 | The Scripps Research Institute | Encoded reaction cassette |
US6100026A (en) * | 1995-04-25 | 2000-08-08 | Irori | Matrices with memories and uses thereof |
US5751629A (en) | 1995-04-25 | 1998-05-12 | Irori | Remotely programmable matrices with memories |
US5763263A (en) * | 1995-11-27 | 1998-06-09 | Dehlinger; Peter J. | Method and apparatus for producing position addressable combinatorial libraries |
US5942443A (en) * | 1996-06-28 | 1999-08-24 | Caliper Technologies Corporation | High throughput screening assay systems in microscale fluidic devices |
US5872015A (en) * | 1996-05-10 | 1999-02-16 | Board Of Trustees Of The University Of Illinois | Molecular diversity screening method |
US5962645A (en) * | 1996-10-04 | 1999-10-05 | University Of Maryland | Antiproliferative factor from patients with interstitial cystitis |
JP3683282B2 (en) | 1996-10-17 | 2005-08-17 | 三菱化学株式会社 | Genotype-phenotype mapping molecule and use thereof |
CN1238366C (en) * | 1997-01-21 | 2006-01-25 | 综合医院公司 | Selection of proteins using RNA-protein fusions |
GB9703369D0 (en) | 1997-02-18 | 1997-04-09 | Lindqvist Bjorn H | Process |
GB9712512D0 (en) | 1997-06-16 | 1997-08-20 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
US5985575A (en) | 1998-05-20 | 1999-11-16 | Wisconsin Alumni Research Foundation | Tethered function assay for protein function |
-
1999
- 1999-08-16 US US09/374,964 patent/US6602685B1/en not_active Expired - Lifetime
- 1999-08-16 AU AU61296/99A patent/AU757955B2/en not_active Expired
- 1999-08-16 DK DK99948042T patent/DK1105360T3/en active
- 1999-08-16 JP JP2000564919A patent/JP4278872B2/en not_active Expired - Lifetime
- 1999-08-16 AT AT99948042T patent/ATE437844T1/en active
- 1999-08-16 EP EP99948042A patent/EP1105360B1/en not_active Expired - Lifetime
- 1999-08-16 DE DE69941193T patent/DE69941193D1/en not_active Expired - Lifetime
- 1999-08-16 WO PCT/US1999/018600 patent/WO2000009464A1/en active IP Right Grant
- 1999-08-16 ES ES99948042T patent/ES2329205T3/en not_active Expired - Lifetime
- 1999-08-16 PT PT99948042T patent/PT1105360E/en unknown
- 1999-08-16 CA CA2337490A patent/CA2337490C/en not_active Expired - Lifetime
-
2009
- 2009-10-13 CY CY20091101052T patent/CY1110515T1/en unknown
Also Published As
Publication number | Publication date |
---|---|
US6602685B1 (en) | 2003-08-05 |
WO2000009464A1 (en) | 2000-02-24 |
PT1105360E (en) | 2009-09-30 |
ATE437844T1 (en) | 2009-08-15 |
JP2002522057A (en) | 2002-07-23 |
JP4278872B2 (en) | 2009-06-17 |
EP1105360A4 (en) | 2005-06-22 |
AU6129699A (en) | 2000-03-06 |
DK1105360T3 (en) | 2009-11-02 |
WO2000009464A9 (en) | 2000-09-28 |
ES2329205T3 (en) | 2009-11-23 |
CA2337490C (en) | 2012-01-03 |
AU757955B2 (en) | 2003-03-13 |
DE69941193D1 (en) | 2009-09-10 |
EP1105360A1 (en) | 2001-06-13 |
EP1105360B1 (en) | 2009-07-29 |
CY1110515T1 (en) | 2015-04-29 |
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